Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| dinotefuran: a potential neonicotinoid insecticide against resistant mosquitoes. | because pyrethroid, organophosphate, and carbamate resistance is more and more developed in mosquitoes of medical importance, there is an urgent need for alternative insecticides for vector control. dinotefuran, a new neonicotinoid insecticide commercialized by mitsui chemicals (tokyo, japan), could be a useful candidate in public health because it shows low mammalian toxicity and great insecticidal activity against a broad range of pests. in this study, the intrinsic toxicity of dinotefuran was ... | 2004 | 15311465 |
| mutations in the bacillus thuringiensis cry1ca toxin demonstrate the role of domains ii and iii in specificity towards spodoptera exigua larvae. | several mutants of the bacillus thuringiensis cry1ca toxin affected with regard to specific activity towards spodoptera exigua were studied. alanine was used to replace single residues in loops 2 and 3 of domain ii (mutant ppb19) and to replace residues 541-544 in domain iii (mutant ppb20). additionally, a cry1ca mutant combining all mutations was constructed (mutant ppb21). toxicity assays showed a marked decrease in toxicity against s. exigua for all mutants, while they retained their activity ... | 2004 | 15320864 |
| seasonal pattern of abundance of aedes aegypti (diptera: culicidae) in buenos aires city, argentina. | in buenos aires, the most crowded city of argentina, there is a potential risk of dengue virus transmission by the mosquito aedes aegypti during late summer. the temporal patterns of oviposition activity and abundance of breeding sites of this vector were studied in two cemeteries of the city. between september 1998 and august 1999, we examined 142 ovitraps weekly and a total of 18,010 water-filled containers. both study areas showed remarkable differences in the percentages of positive ovitraps ... | 2004 | 15322622 |
| a conserved domain of alkaline phosphatase expression in the malpighian tubules of dipteran insects. | malpighian (renal) tubules are key components of the insect osmoregulatory system and show correspondingly great diversity in both number and length. recently, the organisation of the drosophila melanogaster tubule has been elucidated by enhancer trapping, and an array for functional properties has been shown to align with the functional domains. in drosophila, there is a lower tubule domain, which coincides with expression of alkaline phosphatase and delineates the absorptive region of the tubu ... | 2004 | 15326206 |
| mosquito larvicidal properties of essential oil of an indigenous plant, ipomoea cairica linn. | laboratory bioassay of the essential oil extracted from an indigenous plant, ipomoea cairica, commonly known as 'railway creeper', was carried out against the larvae of four vector species of mosquitoes in order to evaluate its mosquito larvicidal effect. bioassay test revealed that the essential oil of the plant possess remarkable larvicidal properties as it could induce 100% mortality in the larvae of culex tritaeniorhynchus (100 ppm), aedes aegypti (120 ppm), anopheles stephensi (120 ppm), an ... | 2004 | 15329451 |
| genetic relationships and evolution of genotypes of yellow fever virus and other members of the yellow fever virus group within the flavivirus genus based on the 3' noncoding region. | genetic relationships among flaviviruses within the yellow fever (yf) virus genetic group were investigated by comparing nucleotide sequences of the 3' noncoding region (3'ncr). size heterogeneity was observed between members and even among strains of the same viral species. size variation between yf strains was due to duplications and/or deletions of repeated nucleotide sequence elements (ryf). west african genotypes had three copies of the ryf (ryf1, ryf2, and ryf3); the angola and the east an ... | 2004 | 15331698 |
| a single amino acid substitution in the envelope protein of chimeric yellow fever-dengue 1 vaccine virus reduces neurovirulence for suckling mice and viremia/viscerotropism for monkeys. | a chimeric yellow fever-dengue 1 (chimerivax-den1) virus was produced by the transfection of vero cells with chimeric in vitro rna transcripts. the cell culture supernatant was subjected to plaque purification for the identification of a vaccine candidate without mutations. of 10 plaque-purified clones, 1 containing no mutation (clone j) was selected for production of the vaccine virus. during subsequent cell culture passaging of this clone for vaccine production, a single amino acid substitutio ... | 2004 | 15331733 |
| intriguing olfactory proteins from the yellow fever mosquito, aedes aegypti. | four antennae-specific proteins (aaegobp1, aaegobp2, aaegobp3, and aaegasp1) were isolated from the yellow fever mosquito, aedes aegypti and their full-length cdnas were cloned. rt-pcr indicated that they are expressed in female and, to a lesser extent, in male antennae, but not in control tissues (legs). aaegobp1 and aaegobp3 showed significant similarity to previously identified mosquito odorant-binding proteins (obps) in cysteine spacing pattern and sequence. two of the isolated proteins have ... | 2004 | 15338030 |
| function-informed transcriptome analysis of drosophila renal tubule. | comprehensive, tissue-specific, microarray analysis is a potent tool for the identification of tightly defined expression patterns that might be missed in whole-organism scans. we applied such an analysis to drosophila melanogaster malpighian (renal) tubule, a defined differentiated tissue. | 2004 | 15345053 |
| critical review of the vector status of aedes albopictus. | the mosquito aedes (stegomyia) albopictus (skuse) (diptera: culicidae), originally indigenous to south-east asia, islands of the western pacific and indian ocean, has spread during recent decades to africa, the mid-east, europe and the americas (north and south) after extending its range eastwards across pacific islands during the early 20th century. the majority of introductions are apparently due to transportation of dormant eggs in tyres. among public health authorities in the newly infested ... | 2004 | 15347388 |
| utilization of pre-existing energy stores of female aedes aegypti mosquitoes during the first gonotrophic cycle. | pre-existing energy reserves may play an important role in regulating the utilization of blood meal proteins in female anautogenous mosquitoes. determining the fate of reserves derived from the sugar meal and larval food during the first gonotrophic cycle would help to elucidate the relative contributions of larval and adult nutrition to survival and reproduction. we measured the allocation of pre-blood-meal reserves to egg production or energy production during the first gonotrophic cycle by us ... | 2004 | 15350611 |
| laboratory and field evaluation of teknar hp-d, a biolarvicidal formulation of bacillus thuringiensis ssp. israelensis, against mosquito vectors. | larvicidal efficacy of teknar hp-d, an improved biolarvicidal formulation of bacillus thuringiensis ssp. israelensis (bti), against anopheles stephensi, culex quinquefasciatus and aedes aegypti was determined in the laboratory, and in field the efficacy of the formulation was tested against cx. quinquefasciatus breeding in cesspits, unused wells and drains. the toxicity of the formulation to gambusia affinis (larvivorous fish), notonecta sp. and diplonychus indicus (water bugs) was also evaluate ... | 2004 | 15350862 |
| experimental evaluation of the relationship between lethal or non-lethal virulence and transmission success in malaria parasite infections. | evolutionary theory suggests that the selection pressure on parasites to maximize their transmission determines their optimal host exploitation strategies and thus their virulence. establishing the adaptive basis to parasite life history traits has important consequences for predicting parasite responses to public health interventions. in this study we examine the extent to which malaria parasites conform to the predicted adaptive trade-off between transmission and virulence, as defined by morta ... | 2004 | 15355551 |
| [evaluation of an educational module on dengue and aedes aegypti for schoolchildren in honduras]. | to evaluate the effectiveness of a special course on environmental health and dengue, given to primary school students and intended to promote behavioral changes in the mothers of those students that would lead to the safe handling of water, adequate disposal of trash, and control of household breeding sites of aedes aegypti, the mosquito vector for dengue. | 2004 | 15357933 |
| [larvicidal activity of tannins isolated of magonia pubescens st. hil. (sapindaceae) against aedes aegypti (diptera, culicidae)]. | phytochemical study of the larvicidal fractions which were carried out for the first time, isolated of the magonia pubescens, monitored by the study of efficacy against the 3rd larval instar of aedes aegypti, in the search of alternatives for the control of that mosquito and to obtain structures susceptible to chemical improving of the activity for the synthetic via of other derived. the fractions with biological activity were monitored chemically through chromatography of thin layer, using as r ... | 2006 | 15361956 |
| [evaluation of the impact on aedes aegypti infestation in cement tanks of the municipal district of canindé, ceará, brazil after using the betta splendens fish as an alternative biological control]. | canindé has a population of 71,235 inhabitants. in april 2001, the city started using larvivorous fish in cement water tanks, as a means of biological control of aedes aegypti larvae. during house-to-house visits by health agents, instead of treating the water tank with larvicide, a betta splendens fish was introduced into each tank. the number of houses and the number of tanks was estimated by monthly surveys. then, the number of tanks per house was determined. taking into account this estimate ... | 2004 | 15361957 |
| stereodivergent construction of cyclic ethers by a regioselective and enantiospecific rhodium-catalyzed allylic etherification: total synthesis of gaur acid. | 0 | 15366088 | |
| the logistic model for predicting the non-gonoactive aedes aegypti females. | to estimate, using logistic regression, the likelihood of occurrence of a non-gonoactive aedes aegypti female, previously fed human blood, with relation to body size and collection method. | 2004 | 15368866 |
| the anterior stomach of larval mosquitoes (aedes aegypti): effects of neuropeptides on transepithelial ion transport and muscular motility. | the present investigation studied the influence of a number of neuropeptides on semi-open preparations of the isolated and perfused anterior stomach of larval aedes aegypti. effects of peptides were observed on the lumen negative transepithelial voltage (vte) that is present with serotonin in the bath; this voltage most likely reflects active hco3- secretion involved in alkalization of the larval anterior stomach. the five different a. aegypti allatostatins (allatostatin a 1-5) all affected vte ... | 2004 | 15371480 |
| a potentially functional mariner transposable element in the protist trichomonas vaginalis. | mariner transposable elements encoding a d,d34d motif-bearing transposase are characterized by their pervasiveness among, and exclusivity to, animal phyla. to date, several hundred sequences have been obtained from taxa ranging from cnidarians to humans, only two of which are known to be functional. related transposons have been identified in plants and fungi, but their absence among protists is noticeable. here, we identify and characterize tvmar1, the first representative of the mariner family ... | 2005 | 15371525 |
| expression of a sterol carrier protein-x gene in the yellow fever mosquito, aedes aegypti. | the sterol carrier protein-x (scp-x), a peroxisomal thiolase/nonspecific lipid binding protein, was characterized in the yellow fever mosquito, aedes aegypti. the aedes aegypti scp-x (aescp-x) has 83% and 75% similarities to drosophila and mammalian scp-x, respectively. however, the aescp-x gene did not produce multiple transcripts, which is characteristic of the vertebrate scp-x gene. levels of aescp-x transcription were higher in larvae and pupae. gut tissue showed the highest level of aescp-x ... | 2004 | 15373808 |
| the aeact-4 gene is expressed in the developing flight muscles of female aedes aegypti. | population reduction of mosquitoes is an effective method for controlling dengue fever and malaria transmission. recent developments in control techniques include proposals to construct transgenic strains of mosquitoes carrying dominant, conditional-lethal genes under the control of sex- and stage-specific promoters. in order to identify such promoters, subtractive cdna libraries derived from male and female pupal mrna of the yellow fever mosquito, aedes aegypti, were constructed and screened. a ... | 2004 | 15373813 |
| some basic biological characteristics of the vector, aedes aegypti, responsible for dengue virus transmission. | 2004 | 15379084 | |
| densonucleosis virus purification by ion exchange membranes. | preparative chromatography is widely used in the downstream purification of biopharmaceutical products. replacement of resins by membranes as chromatographic supports, overcomes many of the limitations associated with resin-based chromatography such as high-pressure drops, slow processing rates due to pore diffusion and channeling of the feed through the bed. in particular, adsorptive membranes may be ideally suited for virus capture. virus capture is critical in a number of applications. in gen ... | 2004 | 15384051 |
| methods to find out the expression of activated genes. | this review deals with the methods of identifying genes that have been activated by inner or outer impulses. the activation and subsequent expression of a gene can be detected by its transcription into a corresponding messenger ribonucleic acid (mrna). principles of the methods for identification of individual activated genes, as well as groups of activated genes are described, the former methods being mostly based on subtractive hybridization and serial analysis of gene expression (sage), the l ... | 2004 | 15385048 |
| rearing of anopheles quadrimaculatus say and aedes aegypti (l.) in the laboratory. | 1949 | 15406584 | |
| aedes aegypti and other mosquito control measures in port sudan. | 1949 | 15408260 | |
| [control of aedes aegypti in chile]. | 1950 | 15411454 | |
| arrest of development of plasmodium gallinaceum in mosquitoes (aedes aegypti) by radiation effect of p32 1,2. | 1950 | 15424666 | |
| effects of juvenile hormone and ecdysone on the timing of vitellogenin appearance in hemolymph of queen and worker pupae of apis mellifera. | the caste-specific regulation of vitellogenin synthesis in the honeybee represents a problem with many yet unresolved details. we carried out experiments to determine when levels of vitellogenin are first detected in hemolymph of female castes of apis mellifera, and whether juvenile hormone and ecdysteroids modulate this process. vitellogenin levels were measured in hemolymph using immunological techniques. we show that in both castes the appearance of vitellogenin in the hemolymph occurs during ... | 2002 | 15455035 |
| aedes aegypti ferritin heavy chain homologue: feeding of iron or blood influences message levels, lengths and subunit abundance. | secreted ferritin in the mosquito, aedes aegypti, has several subunits that are the products of at least two genes, one encoding a homologue of the vertebrate heavy chain (hch) and the other the light chain homologue (lch). here we report the developmental and organ specific pattern of expression of the ferritin hch messages and of both subunit types in control sugar-fed mosquitoes, in those exposed to high levels of dietary iron, and after blood feeding. when northern blots were probed with a h ... | 2002 | 15455041 |
| triacylglycerol-rich lipophorins are found in the dipteran infraorder culicomorpha, not just in mosquitoes. | lipophorin is the major hemolymph protein responsible for lipid transport between tissues of insects. lipophorins from several insect species in order diptera (the fruit fly drosophila melanogaster from the suborder brachycera, the mosquito aedes aegypti; the phantom midges chaoborus maximus and minimus; the black fly simulium vittatum; the crane fly nephrotoma abbreviata, all from the suborder nematocera) were isolated and characterized. all lipophorins consisted of two protein subunits of appr ... | 2002 | 15455049 |
| abstracts of the fourth international symposium on molecular insect science. may 28-june 2, 2002. tucson, arizona, usa. | 2002 | 15455051 | |
| decreased survival of mosquito cells after stable transfection with a drosophila ecdysteroid response element: possible involvement of a 40 kda dna binding protein. | homologous transfection systems provide a useful tool for characterizing promoters and other regulatory elements from cloned genes. we have used cultured aedes albopictus c7-10 mosquito cells to evaluate expression of 20-hydroxyecdysone-inducible genes. although this cell line has previously been shown to synthesize components of the ecdysteroid receptor and ecdysone-inducible proteins, the well-characterized ecdysteroid response element (ecre) from the drosophilahsp27 promoter failed to confer ... | 2002 | 15455055 |
| structure-based analysis of the ultraspiracle protein and docking studies of putative ligands. | the ultraspiracle protein (usp) is the insect ortholog of the mammalian retinoid x receptor (rxr). fundamental questions concern the functional role of usp as the heterodimerization partner of insect nuclear receptors such as the ecdysone receptor. the crystallographic structures of the ligand binding domain of usps of heliothis virescens and drosophila melanogaster solved recently show that helix 12 is locked in an antagonist conformation raising the question whether usps could adopt an agonist ... | 2002 | 15455059 |
| a single gene (yes) controls pigmentation of eyes and scales in heliothis virescens. | a yellow-eyed mutant was discovered in a strain of heliothis virescens, the tobacco budworm, that already exhibited a mutation for yellow scale, y. we investigated the inheritance of these visible mutations as candidate markers for transgenesis. yellow eye was controlled by a single, recessive, autosomal factor, the same type of inheritance previously known for y. presence of the recombinant mutants with yellow scales and wild type eyes in test crosses indicated independent segregation of genes ... | 2001 | 15455061 |
| distribution of ovary ecdysteroidogenic hormone i in the nervous system and gut of mosquitoes. | ovary ecdysteroidogenic hormone i (oeh i) is a gonadotropin in the female mosquito, aedes aegypti. whole-mount immunocytochemistry using oeh i antisera revealed an extensive distribution of immunostained cells in larvae and adults of this mosquito comparable to that observed in the african malaria mosquito, anopheles gambiae. medial neurosecretory cells were stained in brains of larvae and adult ae. aegypti. in an gambiae the lateral neurosecretory cells were stained more often. in both species, ... | 2001 | 15455063 |
| individual and population variation in invertebrates revealed by inter-simple sequence repeats (issrs). | pcr-based molecular markers are well suited for questions requiring large scale surveys of plant and animal populations. inter-simple sequence repeats or issrs are analyzed by a recently developed technique based on the amplification of the regions between inverse-oriented microsatellite loci with oligonucleotides anchored in microsatellites themselves. issrs have shown much promise for the study of the population biology of plants, but have not yet been explored for similar studies of animals. ... | 2001 | 15455068 |
| characterization of an endogenous gene expressed in aedes aegypti using an orally infectious recombinant sindbis virus. | sindbis virus expression vectors have been used successfully to express and silence genes of interest in vivo in several mosquito species, including aedes aegypti, ae. albopictus, ae. triseriatus,culex pipiens, armigeres subalbatus and anopheles gambiae. here we describe the expression of an endogenous gene, defensin, in ae. aegypti using the orally infectious sindbis virus, mre/3'2j expression vector. we optimized conditions to infect mosquito larvae per os using c6/36ae. albopictus cells infec ... | 2001 | 15455070 |
| vaccine candidates derived from a novel infectious cdna clone of an american genotype dengue virus type 2. | a dengue virus type 2 (den-2 tonga/74) isolated from a 1974 epidemic was characterized by mild illness and belongs to the american genotype of den-2 viruses. to prepare a vaccine candidate, a previously described 30 nucleotide deletion (delta30) in the 3' untranslated region of den-4 has been engineered into the den-2 isolate. | 2004 | 15461822 |
| the role of hemagglutinins in the midgut extracts of two lines of aedes aegypti in their susceptibility to dengue-2 virus. | hemagglutinin activity (ha) was studied in the midgut extracts from highly (h) and lowly susceptible strains of aedes aegypti mosquitoes to dengue-2 virus (den-2). ha in the midgut extracts from these two isofemale strains of mosquitoes was high in as compared to (h) mosquitoes. ha was found to be higher with chicken red blood cells (rbcs) than with rabbit and human rbcs of o group. larval midgut extracts showed higher activity than those from adult female mosquitoes. exposure of midgut extracts ... | 2004 | 15462286 |
| saint lucia revisited. | the eastern caribbean island of saint lucia is now famous in parasitological history as the setting for a major programme of schistosomiasis control'. perhaps less well-known are the island's effective control of many intestinal parasites, and elimination of malaria, such that the current patterns of mortality and other demographic indicators now resemble those of industrialized countries. more recently, the island has become the focus for another community-based health programme as the caribbea ... | 1988 | 15463009 |
| satellite dna from the y chromosome of the malaria vector anopheles gambiae. | satellite dna is an enigmatic component of genomic dna with unclear function that has been regarded as "junk." yet, persistence of these tandem highly repetitive sequences in heterochromatic regions of most eukaryotic chromosomes attests to their importance in the genome. we explored the anopheles gambiae genome for the presence of satellite repeats and identified 12 novel satellite dna families. certain families were found in close juxtaposition within the genome. six satellites, falling into t ... | 2005 | 15466420 |
| aromaticity of tyr-202 in the alpha4-alpha5 loop is essential for toxicity of the bacillus thuringiensis cry4a toxin. | the current model for the mechanism of action of the bacillus thuringiensis cry delta-endotoxins involves the penetration of the alpha4-alpha5 hairpin into the target midgut epithelial cell membranes, followed by pore formation. in this study, pcr-based mutagenesis was employed to identify a critical residue within the alpha4-alpha5 loop of the 130kda cry4a mosquito-larvicidal protein. alanine-substitutions of two charged (asp-198 and asp-200) and four polar (asn-190, asn-195, tyr-201 and tyr-20 ... | 2004 | 15469709 |
| comparative larvicidal toxicities of three ecdysone agonists on the mosquitoes aedes aegypti, culex quinquefasciatus, and anopheles gambiae. | ecdysone agonists are hormonally active insect growth regulators that disrupt development of pest insects and have potential for development as insecticides. their effects have been particularly well-studied in lepidoptera and coleoptera, but significantly less is known about their effects on dipterans, particularly aquatic species. the potency of three ecdysone agonists on larvae of 3 mosquito species, aedes aegypti, anopheles gambiae, and culex quinquefasciatus, was examined. anopheles gambiae ... | 2004 | 15484259 |
| [epidemiological characteristics of dengue in the municipality of são luís, maranhão, brazil, 1997-2002]. | a total of 12,008 cases of dengue were reported from 1997 to 2002, with peaks in 1997 (35.8%) and 1998 (45.8%). the disease predominated in the 15-49-year age group (72.2%) and during the rainy season (83.3%), showing a positive correlation with the amount of rainfall (r = 0.84) and relative humidity (r = 0.76) and a negative correlation with temperature (r = -0.78). there was no significant difference (chi2 = 2.319; p > 0.05) in the number of cases in males (49%) and females (51%). dengue was d ... | 2004 | 15486688 |
| yellow fever: the recurring plague. | despite the availability of a safe and efficacious vaccine, yellow fever (yf) remains a disease of significant public health importance, with an estimated 200,000 cases and 30,000 deaths annually. the disease is endemic in tropical regions of africa and south america; nearly 90% of yf cases and deaths occur in africa. it is a significant hazard to unvaccinated travelers to these endemic areas. virus transmission occurs between humans, mosquitoes, and monkeys. the mosquito, the true reservoir of ... | 2004 | 15487593 |
| a preliminary study of multilevel geographic distribution & prevalence of aedes aegypti (diptera: culicidae) in the state of goa, india. | dengue virus activity has never been reported in the state of goa. the present study was carried out to document a multilevel geographic distribution, prevalence and preliminary analysis of risk factors for the invasions of aedes aegypti in goa. | 2004 | 15489555 |
| incomplete surveillance of a dengue-2 epidemic in ibagué, colombia, 1995-1997. | from april 1995 through november 1997 a dengue epidemic occurred in ibagué (400,000 population), colombia, where 3,419 cases were reported and den 2 virus was isolated from seven patients. a sero-survey conducted in 1996 found evidence of previous dengue infection in 9.6% of the population, indicating that many infections had not been reported. the dengue infections occurred in all age groups, but children under five years of age were most frequently infected. forty-five percent of this ibagué p ... | 2004 | 15495597 |
| genetic divergence and dispersal of yellow fever virus, brazil. | an analysis of 79 yellow fever virus (yfv) isolates collected from 1935 to 2001 in brazil showed a single genotype (south america i) circulating in the country, with the exception of a single strain from rondonia, which represented south america genotype ii. brazilian yfv strains have diverged into two clades; an older clade appears to have become extinct and another has become the dominant lineage in recent years. pairwise nucleotide diversity between strains ranged from 0% to 7.4%, while amino ... | 2004 | 15498159 |
| yellow fever virus infectivity for bolivian aedes aegypti mosquitoes. | the absence of urban yellow fever virus (yfv) in bolivian cities has been attributed to the lack of competent urban mosquito vectors. experiments with aedes aegypti from santa cruz, bolivia, demonstrated infection (100%), dissemination (20%), and transmission of a bolivian yfv strain (cenetrop-322). | 2004 | 15498171 |
| yellow fever outbreak, southern sudan, 2003. | in may 2003, an outbreak of fatal hemorrhagic fever, caused by yellow fever virus, occurred in southern sudan. phylogenetic analysis showed that the virus belonged to the east african genotype, which supports the contention that yellow fever is endemic in east africa with the potential to cause large outbreaks in humans. | 2004 | 15498174 |
| acinetobacter baumannii in human body louse. | while we were isolating bartonella quintana from body lice, 40 acinetobacter baumannii strains were also isolated and genotyped. one clone was unique and the other was ampicillin susceptible. a. baumannii dna was later detected in 21% of 622 lice collected worldwide. these findings show an a. baumannii epidemic in human body lice. | 2004 | 15498175 |
| conservation of capa peptide-induced nitric oxide signalling in diptera. | in d. melanogaster malpighian (renal) tubules, the capa peptides stimulate production of nitric oxide (no) and guanosine 3', 5'-cyclic monophosphate (cgmp), resulting in increased fluid transport. the roles of no synthase (nos), no and cgmp in capa peptide signalling were tested in several other insect species of medical relevance within the diptera (aedes aegypti, anopheles stephensi and glossina morsitans) and in one orthopteran out-group, schistocerca gregaria. nos immunoreactivity was detect ... | 2004 | 15498959 |
| [susceptibility of aedes aegypti larvae to the insecticide temephos in the federal district, brazil]. | to study the susceptibility status of aedes aegypti to the organophosphate insecticide temephos. | 2004 | 15499431 |
| [tyrophagus putrescentiae predating adult insects of aedes aegypti and aedes albopictus in laboratory]. | the present study aimed at identifying a mite infesting a colony of aedes aegypti and ae. albopictus as well as investigating the source of infestation. the mite species was identified after it was mounted on slides in hoyer's medium and examined under an optical microscope. it showed to be tyrophagus putrescentiae. fish and rabbit food samples were kept in an environmental chamber at 27 degrees c and 90% humidity for 21 days and were weekly examined. the presence of t. putrescentiae was only ob ... | 2004 | 15499448 |
| control of aedes aegypti with temephos in a buenos aires cemetery, argentina. | the efficacy of a larvicide, temephos, for controlling ae. aegypti was evaluated in a cemetery in buenos aires, argentina. breeding sites decreased from 18.4% in the first study period (nov 1998 to may 1999, without temephos) to 2.2% in the second period (nov 1999 to may 2000, two applications), and to 0.05% in the third one (nov 2000 to may 2001, five applications). ovitraps with eggs decreased from 17% in the first period to 5.8% in the second period, and to 2.9% in the third one. results sugg ... | 2004 | 15499449 |
| dengue emergence and adaptation to peridomestic mosquitoes. | phylogenetic evidence suggests that endemic and epidemic dengue viruses (denv), transmitted among humans by the anthropophilic mosquitoes aedes aegypti and ae. albopictus, emerged when ancestral, sylvatic denv transmitted among nonhuman primates by sylvatic aedes mosquitoes adapted to these peridomestic vectors. we tested this hypothesis by retrospectively examining evidence for adaptation of epidemic and endemic versus sylvatic strains of denv-2 to ae. albopictus and ae. aegypti. first and seco ... | 2004 | 15504265 |
| chemical composition and larvicidal activity of the essential oils from eupatorium betonicaeforme (d.c.) baker (asteraceae). | the volatile composition of the essential oils from leaves and roots of eupatorium betonicaeforme (d.c.) baker was analyzed by gc-ms. a total of 12 compounds were identified. beta-caryophyllene (12.4-41.7%), alpha-humulene (11.7-14.6%), gamma-muurolene (10.4-19.0%), bicyclogermacrene (15.0-17.5%), 2,2-dimethyl-6-vinylchroman-4-one (10.3-25.5%), and 2-senecioyl-4-vinylphenol (8.5-41.0%) were the most prominent constituents. the former two compounds were isolated and characterized by spectroscopic ... | 2004 | 15506805 |
| serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor. | dengue virus, the causative agent of dengue fever, dengue shock syndrome, and dengue hemorrhagic fever, infects susceptible cells by initially binding to a receptor(s) located on the host cell surface. evidence to date suggests that receptor usage may be cell and serotype specific, and this study sought to identify dengue virus serotype 1 binding proteins on the surface of liver cells, a known target organ. by using a virus overlay protein binding assay (vopba), in both nondenaturing and denatur ... | 2004 | 15507651 |
| study of sequence variation of dengue type 3 virus in naturally infected mosquitoes and human hosts: implications for transmission and evolution. | dengue virus is an arbovirus that replicates alternately in the mosquito vector and human host. we investigated sequences of dengue type 3 virus in naturally infected aedes aegypti mosquitoes and in eight patients from the same outbreak and reported that the extent of sequence variation seen with the mosquitoes was generally lower than that seen with the patients (mean diversity, 0.21 versus 0.38% and 0.09 versus 0.23% for the envelope [e] and capsid [c] genes, respectively). this was further ve ... | 2004 | 15507664 |
| nosocomial transmission of dengue. | 2004 | 15515246 | |
| population dynamics of aedes aegypti from a dengue hyperendemic urban setting in colombia. | this study evaluated if the aedes aegypti population in the city of cali, colombia was composed of genetically distinct local populations with different levels of insecticide resistance and dengue vector competence. insecticide resistance was assayed biochemically and was associated with varying levels of mixed-function oxidases and non-specific esterases. the genes encoding those enzymes were under selective pressure from insecticides used to suppress ae. aegypti populations. vector competence ... | 2004 | 15516650 |
| molecular differentiation of colonized human malaria vectors by 28s ribosomal dna polymorphisms. | anopheles gambiae s.s. giles, an. stephensi liston, an. freeborni aitken, and an. quadrimaculatus say are cultured and studied in molecular genetic and transgenic laboratories with increasing frequency. with limited research space, these mosquitoes are often maintained in the same insectary. under these conditions, cross-contamination of colonies can occur and have devastating consequences to affected research programs. we have developed a polymerase chain reaction-based assay targeting the 28s ... | 2004 | 15516651 |
| new insecticidal amides from petroleum ether extract of dried piper nigrum l. whole fruits. | the petroleum ether extract of dried ground whole fruits of piper nigrum l. afforded 20 compounds (1-20) including two new insecticidal amides named as pipnoohine (1), and pipyahyine (2), seven reported for the first time from this plant (12, 13, 15-17, 19, 20), and eleven known compounds (3-11, 14, 18). the structure of 1 has been elucidated as (2e,4e,12z)-n-(4-methylpentyl)octadeca-2,4,12-trienamide and that of 2 as (2e,4e,11e)-12-(benzo[1,3]dioxol-5-yl)-n-(3-methylbutyl)dodeca-2,4,11-trien-am ... | 2004 | 15516761 |
| essential role of membrane-attack protein in malarial transmission to mosquito host. | after ingestion of infected blood by a mosquito, malarial parasites are fertilized in the mosquito midgut and develop into motile ookinetes. these ookinetes invade epithelial cells by rupturing the cell membrane and migrate through the cytoplasm toward the basal lamina, on which they develop to oocysts. here we report that a microneme protein with a membrane-attack complex and perforin (macpf)-related domain, which we name membrane-attack ookinete protein (maop), is produced in the ookinete stag ... | 2004 | 15520375 |
| natively unfolded domains in endocytosis: hooks, lines and linkers. | it is commonly assumed that a protein must adopt a tertiary structure to achieve its active native state and that regions of a protein that are devoid of alpha-helix or beta-sheet structures are functionally inert. although extended proline-rich regions are recognized as presenting binding motifs to, for example, src homology 2 (sh2) and sh3 domains, the idea persists that natively unfolded regions in functional proteins are simply 'spacers' between the folded domains. such a view has been chall ... | 0 | 15520805 |
| molecular characterization of a novel peroxidase involved in aedes aegypti chorion protein crosslinking. | peroxidase-catalyzed chorion or eggshell protein crosslinking is an important biochemical event contributing to the formation of a protective chorion or eggshell in insects. although the survival of the developing or developed embryo in the environment before hatching relies on the protection of the chorion, the identity of the peroxidative enzyme responsible for mediating chorion protein crosslinking has never been identified in any insect species. in this report, we describe the determination ... | 2004 | 15522615 |
| field- and laboratory-based active dengue surveillance in chennai, tamil nadu, india: observations before and during the 2001 dengue epidemic. | dengue cases are reported every year in the city of chennai, tamil nadu, india. since april 2001, longitudinal field- and laboratory-based active dengue surveillance has been carried out in chennai to study dengue trends. | 2004 | 15525913 |
| molecular characterization of brevibacillus laterosporus and its potential use in biological control. | thirty-three strains of brevibacillus laterosporus, including three novel strains isolated from brazilian soil samples, were examined for genetic variability by the use of different pcr-based methods. molecular markers that could characterize bacterial strains with regards to their pathogenic potential were investigated. in addition, toxicity was assessed by the use of insects belonging to the orders lepidoptera and coleoptera and the mollusk biomphalaria glabrata. among the targets tested, biom ... | 2004 | 15528531 |
| entomological observations on dengue vector mosquitoes following a suspected outbreak of dengue in certain parts of nagaland with a note on their susceptibility to insecticides. | three species of aedes viz., aedes albopictus, aedes aegypti and aedes annandalei were detected from different breeding sources in and around human habitats during entomological study conducted following an outbreak suspected to be of dengue (which occurred during, 1994) in parts of medziphema phc area of nagaland in two different points of time ie., in the year, 1994 and, 2000. the potential dengue vector, aedes albopictus showed high preponderance by breeding in all types of containers searche ... | 2004 | 15529881 |
| larvicidal action of ethanolic extracts from fruit endocarps of melia azedarach and azadirachta indica against the dengue mosquito aedes aegypti. | ethanolic extracts from the kernels of ripe fruits from the indian lilac melia azedarach and from the well-known neem tree, azadirachta indica were assayed against larvae of aedes aegypti, the mosquito vector of dengue fever. the lethality bioassays were carried out according to the recommendations of the world health organization. extracts were tested at doses ranging from 0.0033 to 0.05 g% in an aqueous medium for 24 and 48 h, at 25 or 30 degrees c, with or without feeding of the larvae. lc50, ... | 2004 | 15530964 |
| repellency of aerosol and cream products containing fennel oil to mosquitoes under laboratory and field conditions. | the repellency of fennel (foeniculum vulgare miller)-containing products (5% aerosol and 8% cream) against mosquitoes was compared with those of citronella oil, geranium oil and deet, as well as three commercial repellents, baby keeper cream containing ir3535, meimei cream containing citronella and geranium oils, and repellan s aerosol containing 19% n,n-diethyl-m-toluamide (deet) under laboratory and field conditions. in a laboratory study with female aedes aegypti (l), fennel oil exhibited goo ... | 2004 | 15532688 |
| efficacy of methyl bromide fumigation and insecticidal dips against aedes eggs on lucky bamboo. | importation of lucky bamboo (dracaena sp.) stalk clusters has led to the accidental importation of aedes albopictus in some countries. current methods of fumigation using methyl bromide are harmful to live plants and thus are not acceptable. we exposed aedes aegypti eggs to methyl bromide at rates of 32 g/m3 for 2 and 3 h and 48 g/m3 for 3 h, achieving complete control in 0/5, 1/5, and 5/5 replicates, respectively. submersion for 1 min in aqueous solutions of 0.04% active ingredient (ai) lambda- ... | 2004 | 15532928 |
| comparative efficacy of ir3535 and deet as repellents against adult aedes aegypti and culex quinquefasciatus. | arm-in-cage laboratory evaluations of 2 proprietary formulations of the mosquito repellents ir3535 and n,n-diethyl-3-methylbenzamide (deet; aqueous cream, hydroalcoholic spray) were made with 10 and 20% concentrations of each repellent. also, 4 commercially available products containing ir3535 (expedition insect repellent 20.07% active ingredient [ai], bug guard plus with spf30 sunscreen 7.5% ai, bug guard plus with spf15 sunscreen 7.5% ai, and bug guard plus 7.5% ai) were tested. all comparison ... | 2004 | 15532931 |
| larvicidal persistence of formulations of bacillus thuringiensis var. israelensis to control larval aedes aegypti. | after detection of resistance to the organophosphate temephos in populations of aedes aegypti in brazil, corncob granule (cg) and water-dispersible granule (wdg) formulations of bacillus thuringiensis var. israelensis (bti) were introduced in routine focal treatments. larvicidal persistence and the influence of exposure to sunlight on vectobac formulations of bti were compared in 250-liter fiberglass water containers. production of pupal ae. aegypti in containers was used to indicate control. in ... | 2004 | 15532933 |
| invertebrate carcasses as a resource for competing aedes albopictus and aedes aegypti (diptera: culicidae). | terrestrial invertebrate carcasses are an important resource for insects developing in pitcher plants. however, little is known of the role of these carcasses in other containers, which also receive leaf fall and stemflow inputs. this experiment investigated effects of accumulated invertebrate carcasses as a resource for two competing mosquitoes, aedes albopictus (skuse) and aedes aegypti (l.), whether either species differentially benefited from accumulated carcasses, and if such a benefit affe ... | 0 | 15535579 |
| experimental detection of rift valley fever virus by reverse transcription-polymerase chain reaction assay in large samples of mosquitoes. | a reverse transcription-polymerase chain reaction (rt-pcr) was assessed in laboratory tests to detect the presence of single aedes aegypti (l.) or eretmapodites quinquevittatus theobald mosquitoes infected with rift valley fever virus in pools of mosquitoes, 50-600 in size, from laboratory colonies or mixed field collections. the viral rna was detected in all pools containing infected mosquitoes and was shown to be as sensitive as infant mice but more sensitive than vero cell cultures for virus ... | 2000 | 15535594 |
| photoelectric sensing device for recording mosquito host-seeking behavior in the laboratory. | a new automatic device for recording the host-seeking behavior of mosquitoes was designed using photoelectric sensors. the host-seeking rhythms of several species of mosquitoes were recorded under laboratory conditions. use of co2, in addition to heating and black color to activate mosquito flight, proved to be important for evaluation of the present recording device. the diel rhythms of nonblood-fed aedes albopictus (skuse), aedes aegypti (l.), culex quinquefasciatus say, culex tritaeniorhynchu ... | 2004 | 15535615 |
| synergy between toxins of bacillus thuringiensis subsp. israelensis and bacillus sphaericus. | synergistic interactions among the multiple endotoxins of bacillus thuringiensis subsp. israelensis de barjac play an important role in its high toxicity to mosquito larvae and the absence of insecticide resistance in populations treated with this bacterium. a lack of toxin complexity and synergism are the apparent causes of resistance to bacillus sphaericus neide in particular culex field populations. to identify endotoxin combinations of the two bacillus species that might improve insecticidal ... | 2004 | 15535624 |
| susceptibility of urban and rural populations of aedes albopictus from são paulo state, brazil, to infection by dengue-1 and -2 viruses. | in brazil, dengue viruses (denv) are transmitted by aedes aegypti (l.). however, the recent introduction of aedes albopictus (skuse) has caused concern, because some populations of this species also are capable of denv transmission. ae. albopictus from rural and urban localities of são paulo state, brazil, were artificially exposed to infection with denv-1 and -2 to evaluate their susceptibility. the rates of infection (6.2 and 5.7% for denv-1; 5.3 and 12% for denv-2) and dissemination (22.2 and ... | 2004 | 15535628 |
| immune responses to mosquito saliva in 14 individuals with acute systemic allergic reactions to mosquito bites. | mosquito bite-induced acute systemic allergic reactions are an increasing clinical concern and have not been optimally characterized immunologically. | 2004 | 15536430 |
| saliva of the yellow fever mosquito, aedes aegypti, modulates murine lymphocyte function. | saliva of many vector arthropods contains factors that inhibit haemostatic responses in their vertebrate hosts. less is known about the effect of vector saliva on host immune responses. we investigated the effect of aedes aegypti salivary gland extracts on antigen-stimulated responses of transgenic ova-tcr do11 mouse splenocytes in vitro. t-cell proliferation was inhibited in a dose-dependent manner, with greater than 50% inhibition at 0.3 salivary gland pair (sgp) equivalents/ml. lps-stimulated ... | 2004 | 15541033 |
| weather-based prediction of plasmodium falciparum malaria in epidemic-prone regions of ethiopia i. patterns of lagged weather effects reflect biological mechanisms. | malaria epidemics due to plasmodium falciparum are reported frequently in the east african highlands with high case fatality rates. there have been formal attempts to predict epidemics by the use of climatic variables that are predictors of transmission potential. however, little consensus has emerged about the relative importance and predictive value of different factors. understanding the reasons for variation is crucial to determining specific and important indicators for epidemic prediction. ... | 2004 | 15541174 |
| chimpanzee fab fragments and a derived humanized immunoglobulin g1 antibody that efficiently cross-neutralize dengue type 1 and type 2 viruses. | passive immunization with monoclonal antibodies from humans or nonhuman primates represents an attractive alternative to vaccines for prevention of illness caused by dengue viruses (denv) and other flaviviruses, including the west nile virus. in a previous study, repertoire cloning to recover fab fragments from bone marrow mrna of chimpanzees infected with all four denv serotypes (dengue virus serotype 1 [denv-1] to denv-4) was described. in that study, a humanized immunoglobulin g1 (igg1) antib ... | 2004 | 15542643 |
| bracoviruses contain a large multigene family coding for protein tyrosine phosphatases. | the relationship between parasitic wasps and bracoviruses constitutes one of the few known mutualisms between viruses and eukaryotes. the virions produced in the wasp ovaries are injected into host lepidopteran larvae, where virus genes are expressed, allowing successful development of the parasite by inducing host immune suppression and developmental arrest. bracovirus-bearing wasps have a common phylogenetic origin, and contemporary bracoviruses are hypothesized to have been inherited by chrom ... | 0 | 15542661 |
| evaluation of mesocyclops annulatus (copepoda: cyclopoidea) as a control agent of aedes aegypti (diptera: culicidae) in argentina. | we evaluated the potential of mesocyclops annulatus as a control agent of aedes aegypti in la plata city (argentina). mosquito larval survivorship due to predation by these copepods was estimated at weekly intervals during the oviposition period of a. aegypti. mean weekly a. aegypti larval survivorship in cylindrical plastic containers (12 cm height and 11 cm diameter) with copepods was significantly lower than in control containers. furthermore, weekly larval survival was negatively correlated ... | 2004 | 15543420 |
| larvicidal activity of essential oils from brazilian plants against aedes aegypti l. | aedes aegypti l. is the major vector of dengue fever, an endemic disease in brazil. in an effort to find effective and affordable ways to control this mosquito, the larvicidal activities of essential oils from nine plants widely found in the northeast of brazil were analyzed by measurement of their lc50. the essential oils were extracted by steam distillation and their chemical composition determined by gl-chromatography coupled to mass spectroscopy. the essential oils from cymbopogon citratus a ... | 2004 | 15543421 |
| the role of phenylalanine hydroxylase in melanotic encapsulation of filarial worms in two species of mosquitoes. | melanin formation has a significant influence on mosquito vector competence by limiting the development of metazoan parasites. tyrosine, the rate-limiting substrate of melanin production, can be obtained exogenously or derived from phenylalanine by phenylalanine hydroxylase (pah). the characteristics of this defense mechanism, such as temporal expression of constituent enzymes involved in the biosynthetic pathway, can vary considerably between mosquito species. we investigated the functional rol ... | 2004 | 15544946 |
| an active transposable element, herves, from the african malaria mosquito anopheles gambiae. | transposable elements have proven to be invaluable tools for genetically manipulating a wide variety of plants, animals, and microbes. some have suggested that they could be used to spread desirable genes, such as refractoriness to plasmodium infection, through target populations of anopheles gambiae, thereby disabling the mosquito's ability to transmit malaria. to achieve this, a transposon must remain mobile and intact after the initial introduction into the genome. endogenous, active class ii ... | 2005 | 15545643 |
| weather-based prediction of plasmodium falciparum malaria in epidemic-prone regions of ethiopia ii. weather-based prediction systems perform comparably to early detection systems in identifying times for interventions. | timely and accurate information about the onset of malaria epidemics is essential for effective control activities in epidemic-prone regions. early warning methods that provide earlier alerts (usually by the use of weather variables) may permit control measures to interrupt transmission earlier in the epidemic, perhaps at the expense of some level of accuracy. | 2004 | 15555061 |
| cysteine and keto acids modulate mosquito kynurenine aminotransferase catalyzed kynurenic acid production. | kynurenine aminotransferase (kat) catalyzes the formation of kynurenic acid (kyna), the natural antagonist of ionotropic glutamate receptors. this study tests potential substrates and assesses the effects of amino acids and keto acids on the activity of mosquito kat. various keto acids, when simultaneously present in the same reaction mixture, display a combined effect on kat catalyzed kyna production. moreover, methionine and glutamine show inhibitory effects on kat activity, while cysteine fun ... | 0 | 15556614 |
| characterization of a defensin from the sand fly phlebotomus duboscqi induced by challenge with bacteria or the protozoan parasite leishmania major. | antimicrobial peptides are major components of the innate immune response of epithelial cells. in insect vectors, these peptides may play a role in the control of gut pathogens. we have analyzed antimicrobial peptides produced by the sand fly phlebotomus duboscqi, after challenge by injected bacteria or feeding with bacteria or the protozoan parasite leishmania major. a new hemolymph peptide with antimicrobial activity was identified and shown to be a member of the insect defensin family. intere ... | 2004 | 15557638 |
| association of human leukocyte antigen dq1 and dengue fever in a white southern brazilian population. | dengue is an infectious disease of viral etiology transmitted by the mosquitoes aedes aegypti, a. albopictus, and a. scutellaris. it can develop either as a benign form or as a severe hemorrhagic form. previous work showed an association of the hemorrhagic form with human leukocyte antigens (hla), suggesting a role of genetic factors in disease susceptibility. nevertheless, data on hla association with the classical form of the disease is scarce in literature. sixty-four patients and 667 normal ... | 2004 | 15558162 |
| freeze-fracture and immunogold analysis of aquaporin-4 (aqp4) square arrays, with models of aqp4 lattice assembly. | each day, approximately 0.5-0.9 l of water diffuses through (primarily) aquaporin-1 (aqp1) channels in the human choroid plexus, into the cerebrospinal fluid of the brain ventricles and spinal cord central canal, through the ependymal cell lining, and into the parenchyma of the cns. additional water is also derived from metabolism of glucose within the cns parenchyma. to maintain osmotic homeostasis, an equivalent amount of water exits the cns parenchyma by diffusion into interstitial capillarie ... | 2004 | 15561408 |
| laboratory studies on the predatory potential of dragon-fly nymphs on mosquito larvae. | biocontrol potential of dragonfly nymph, brachythemis contaminata fabricius against the larvae of anopheles stephensi, culex. quinquefasciatus and aedes aegypti was studied under laboratory conditions. it was found that dragonfly nymph had highest predation efficacy against an. stephensi followed by cx. quinquefasciatus and ae. aegypti. feeding rate increased with decrease in prey size/stage. analysis of data indicated that dragonfly nymphs have good predatory potential and can be used as a biol ... | 2003 | 15562955 |
| susceptibility of two different strains of aedes aegypti (diptera: culicidae) to plant oils. | the toxicity of 11 oils extracted from plants commonly grown in the himalayan region was studied using larvae of two aedes aegypti (l.) strains. a strain from liverpool, england, was highly susceptible to these oils. the lc50 values were much higher in a local laboratory strain. daucus carota l. oil was highly toxic in both strains. differences in the susceptibility of these strains to the action of the test oils and their potential use in integrated pest management are discussed. | 2004 | 15568366 |
| a gpi-linked carbonic anhydrase expressed in the larval mosquito midgut. | we have previously described the first cloning and partial characterization of carbonic anhydrase from larval aedes aegypti mosquitoes. larval mosquitoes utilize an alkaline digestive environment in the lumen of their anterior midgut, and we have also demonstrated a critical link between alkalization of the gut and carbonic anhydrase(s). in this report we further examine the nature of the previously described carbonic anhydrase and test the hypothesis that its pattern of expression is consistent ... | 2004 | 15579552 |
| functional characterisation of the anopheles leucokinins and their cognate g-protein coupled receptor. | identification of the anopheles gambiae leucokinin gene from the completed a. gambiae genome revealed that this insect species contains three leucokinin peptides, named anopheles leucokinin i-iii. these peptides are similar to those identified in two other mosquito species, aedes aegypti and culex salinarius. additionally, anopheles leucokinin i displays sequence similarity to drosophila melanogaster leucokinin. using a combination of computational and molecular approaches, a full-length cdna fo ... | 2004 | 15579553 |
| population genetic structure of anopheles gambiae mosquitoes on lake victoria islands, west kenya. | understanding the genetic structure of island anopheles gambiae populations is important for the current tactics in mosquito control and for the proposed strategy using genetically-modified mosquitoes (gmm). genetically-isolated mosquito populations on islands are a potential site for testing gmm. the objective of this study was to determine the genetic structure of a. gambiae populations on the islands in lake victoria, western kenya. | 2004 | 15581429 |