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[impact of implementation of three gorges project on schistosomiasis endemic situation and control in nanjing city].to understand the changes of hydrological regime in the yangtze river and schistosomiasis endemic situation in nanjing city after the implementation of three gorges project.201527097474
screening and identification of dna aptamers toward schistosoma japonicum eggs via selex.schistosomiasis is a major parasitic disease caused by blood flukes of the genus schistosoma. several million people all over the world are estimated to suffer from severe morbidity as a consequence of schistosomiasis. the worm's eggs, which cause the symptoms of schistosomiasis, are generally used to diagnose the disease. in this study, we employed egg-based systematic evolution of ligands by exponential enrichment (egg-selex) and identified a panel of ssdna aptamers specifically binding to egg ...201627121794
comprehensive transcriptome analysis of sex-biased expressed genes reveals discrete biological and physiological features of male and female schistosoma japonicum.schistosomiasis is a chronic and debilitating disease caused by blood flukes (digenetic trematodes) of the genus schistosoma. schistosomes are sexually dimorphic and exhibit dramatic morphological changes during a complex lifecycle which requires subtle gene regulatory mechanisms to fulfil these complex biological processes. in the current study, a 41,982 features custom dna microarray, which represents the most comprehensive probe coverage for any schistosome transcriptome study, was designed b ...201627128440
evolution of the national schistosomiasis control programmes in the people's republic of china.schistosomiasis japonica is caused by the parasitic trematode schistosoma japonicum. it is endemic in the people's republic of china and has significant impact on human health and socioeconomic development in certain regions. over the last six decades, the national control programmes evolved in remarkable ways and brought schistosomiasis japonica largely under control. we describe the history and evolution of schistosomiasis control in the people's republic of china, with an emphasis on shifts i ...201627137441
the establishment and function of schistosomiasis surveillance system towards elimination in the people's republic of china.schistosoma japonicum is the main schistosome species in the people's republic of china, causing intestinal schistosomiasis, a debilitating disease of public health importance. the people's republic of china used to be heavily endemic with schistosomiasis, but great progress has been made through the vigorous efforts of the national control programmes in the last six decades. presently, efforts are geared towards eliminating schistosomiasis from the people's republic of china by the end of 2025 ...201627137445
biology and control of snail intermediate host of schistosoma japonicum in the people's republic of china.schistosomiasis caused by schistosoma japonicum is a severe parasitic disease in the people's republic of china and imposed considerable burden on human and domestic animal health and socioeconomic development. the significant achievement in schistosomiasis control has been made in last 60years. oncomelania hupensis as the only intermediate host of s. japonicum plays a key role in disease transmission. the habitat complexity of the snails challenges to effective control. in this review we share ...201627137448
integrated control strategy of schistosomiasis in the people's republic of china: projects involving agriculture, water conservancy, forestry, sanitation and environmental modification.among the three major schistosome species infecting human beings, schistosoma japonicum is the only endemic species in the people's republic of china. schistosomiasis is endemic in 78 countries and regions and poses a severe threat to public health and socioeconomic development. through more than 60years of hard work and endeavour, the people's republic of china has made considerable achievements and reduced the morbidity and prevalence of this disease to the lowest level ever recorded, especial ...201627137449
towards the elimination of schistosomiasis japonica through control of the disease in domestic animals in the people's republic of china: a tale of over 60years.schistosomiasis japonica, an endemic, zoonotic tropical parasitic disease caused by schistosoma japonicum, remains an important public health concern in the people's republic of china. unlike other species of schistosoma, over 40 species of wild and domestic animals can act as reservoir hosts of s. japonicum, which increases the difficulty for the control of this tropical disease. it is widely recognized that domestic animals, particularly water buffaloes and cattle, play an important role in th ...201627137450
health education as an important component in the national schistosomiasis control programme in the people's republic of china.schistosomiasis control programme in the people's republic of china had promoted the mass mobilization of health education in various forms, such as films, drama, traditional opera, poems, slogans, posters, exhibits. this paper is trying to review the impacts of those forms on different endemic settings and targeted populations. in the future, health education and health promotion will still be the effective strategy and one of the interventions in the national control programme for schistosomia ...201627137451
development and application of diagnostics in the national schistosomiasis control programme in the people's republic of china.schistosomiasis, caused by schistosoma japonicum infection to human, has a documented history of more than 2100years in the people's republic of china. in spite of great progress in controlling the disease, it is still one of the most serious parasitic diseases in the country. the study and use of diagnostic techniques play an important role in the targeting of chemotherapy that has been continuously applied in the national schistosomiasis control programme for several decades. this paper review ...201627137454
toxoplasma gondii gra15ii effector-induced m1 cells ameliorate liver fibrosis in mice infected with schistosomiasis japonica.recent studies indicated that type ii toxoplasma gondii (tg) gra15ii favored the generation of classically activated macrophages (m1), whereas type i/iii tgrop16i/iii promoted the polarization of alternatively activated macrophages (m2). a number of studies have demonstrated that m2 cells are involved in the pathogenesis of the liver fibrogenesis caused by schistosoma japonicum. the purpose of the present study was to explore the inhibitory effect of toxoplasma-derived tggra15ii on mouse hepatic ...201627157496
ultrasonography of gallbladder abnormalities due to schistosomiasis.after malaria, schistosomiasis remains the most important tropical parasitic disease in large parts of the world. schistosomiasis has recently re-emerged in southern europe. intestinal schistosomiasis is caused by most schistosoma (s.) spp. pathogenic to humans and leads to chronic inflammation and fibrosis of the colon as well as to liver fibrosis. gallbladder abnormalities usually occur in patients with advanced hepatic portal fibrosis due to schistosoma mansoni infection. occasionally, gallbl ...201627169865
molecular characterization of s. japonicum exosome-like vesicles reveals their regulatory roles in parasite-host interactions.secreted extracellular vesicles play an important role in pathogen-host interactions. increased knowledge of schistosome extracellular vesicles could provide insights into schistosome-host interactions and enable the development of novel intervention strategies to inhibit parasitic processes and lessen disease transmission. here, we describe biochemical characterization of schistosoma japonicum exosome-like vesicles (s. japonicum evs). a total of 403 proteins were identified in s. japonicum evs, ...201627172881
protection and immunological study on two tetraspanin-derived vaccine candidates against schistosomiasis japonicum.tetraspanins (tsps) are proteins found on the surface of helminth parasites of the genus schistosoma and are regarded as potentially protective antigens. the large extracellular loop of schistosoma mansoni tetraspanin-2, sm-tsp-2, when fused to a thioredoxin partner and formulated with freund's adjuvants, has been shown to be an efficacious vaccine against murine schistosomiasis. it is well recognized that cd4(+) t-cell-dependent immunity might play an important role against schistosomes; howeve ...201627189226
saposin-like proteins, a multigene family of schistosoma species, are biomarkers for the immunodiagnosis of schistosomiasis japonica.one major obstacle to schistosomiasis prevention and control is the lack of accurate and sensitive diagnostic approaches, which are essential for planning, targeting, and evaluating disease control efforts.201627190177
genetic variation between schistosoma japonicum lineages from lake and mountainous regions in china revealed by resequencing whole genomes.schistosoma infection is a major cause of morbidity and mortality worldwide. schistosomiasis japonica is endemic in mainland china along the yangtze river, typically distributed in two geographical categories of lake and mountainous regions. study on schistosome genetic diversity is of interest in respect of understanding parasite biology and transmission, and formulating control strategy. certain genetic variations may be associated with adaptations to different ecological habitats. the aim of ...201627207135
characteristics of schistosoma japonicum infection induced ifn-γ and il-4 co-expressing plasticity th cells.schistosoma japonicum infection can induce granulomatous inflammation and cause tissue damage in the mouse liver. the cytokine secretion profile of t helper (th) cells depends on both the nature of the activating stimulus and the local microenvironment (e.g. cytokines and other soluble factors). in the present study, we found an accumulation of large numbers of ifn-γ(+)  il-4(+)  cd4(+) t cells in mouse livers. this ifn-γ(+)  il-4(+) cell population increased from 0·68 ± 0·57% in uninfected mice ...201627242265
t follicular helper cells in patients with acute schistosomiasis.the role of t follicular helper (tfh) cells in schistosome infection is not fully defined. in a previous study, a higher frequency of circulating pd-1(+)cxcr5(+)cd4(+) tfh cells was observed in patients with chronic schistosomiasis relative to healthy controls (hcs) and it correlated positively with the level of soluble egg antigen (sea) specific antibodies in serum. however, the function of tfh cells in patients with acute schistosomiasis remains elusive; this was investigated in the present st ...201627266984
functional characterisation of schistosoma japonicum acetylcholinesterase.acetylcholinesterase (ache) is an important metabolic enzyme of schistosomes present in the musculature and on the surface of the blood stage where it has been implicated in the modulation of glucose scavenging from mammalian host blood. as both a target for the antischistosomal drug metrifonate and as a potential vaccine candidate, ache has been characterised in the schistosome species schistosoma mansoni, s. haematobium and s. bovis, but not in s. japonicum. recently, using a schistosome prote ...201627283196
acute appendicitis caused by schistosoma japonicum. 201627283925
[study on immune status of patients with schistosomiasis japonica in poyang lake region iii humoral and cellular immune characteristics between schistosoma japonicum high and low antibody responders].to explore the immune mechanism of negative results of immune tests of schistosomiasis japonica patients.201627356398
[establishment and evaluation of loop-mediated isothermal amplification based on plasmodium falciparum unique genes coding phist proteins].to establish a novel convenient loop-mediated isothermal amplification (lamp) method with the unique genes coding plasmodium helical interspersed sub-telomeric superfamily (phist) for the rapid molecular diagnosis of p. falciparum.201627356403
[studies on immunogenicity and immunoprotection induced by heat shock protein 60 kda of schistosoma japonicum in mice].to evaluate the immunogenicity and immunoprotective effect of heat shock protein 60 kda (sjhsp60) of schistosoma japonicum in mice after immunization and challenge infection, and explore the mechanism.201627356404
[analysis of schistosomiasis endemic situation in yizhou city from 2011 to 2014].to evaluate the effect of schistosomiasis control in yizhou city, guangxi zhuang autonomous region, from 2011 to 2014, so as to provide the evidence for formulating the further prevention and control strategy.201627356418
pentoxifylline inhibits liver fibrosis via hedgehog signaling pathway.infection of schistosomiasis japonica may eventually lead to liver fibrosis, and no effective antifibrotic therapies are available but liver transplantation. hedgehog (hh) signaling pathway has been involved in the process and is a promising target for treating liver fibrosis. this study aimed to explore the effects of pentoxifylline (ptx) on liver fibrosis induced by schistosoma japonicum infection by inhibiting the hh signaling pathway. phorbol12-myristate13-acetate (pma) was used to induce hu ...201627376806
the three gorges dam: does it accelerate or delay the progress towards eliminating transmission of schistosomiasis in china?the three gorges dam, located in the largest endemic area of schistosomiasis in china, is one of the world's largest hydroelectric projects to date. some large-scale hydro projects have resulted in schistosomiasis emergence or re-emergence. therefore, the dam's potential impact on the transmission of schistosoma japonicum has raised concerns from medical researchers worldwide. a systematic literature review, coupled with an analysis of data on the water level and snail density in the yangtze riv ...201627377962
recombinant t2 rnase protein of schistosoma japonicum inhibits expression of α-sma in lx-2 cells.recombinant t2 rnase glycoprotein, which showed a certain degree of homology to omega-1 from schistosoma mansoni eggs, was expressed in adult worms of schistosoma japonicum, but not in eggs of s. japonicum. the direct biological role of the recombinant t2 rnase protein in activation of hepatic stellate cells (hscs) remains unknown. in the present study, the immortalized human hsc line (lx-2 cells) was treated with the recombinant t2 rnase protein at indicated concentrations for various time poin ...201627384176
schistosoma japonicum cystatin attenuates murine collagen-induced arthritis.recombinant sjcystatin (rsjcystatin), a recombinant protein of schistosoma japonicum cystatin, has been reported to have an effect on immunoregulation mediated by il-10 induction. rheumatoid arthritis (ra) is a common autoimmune inflammatory arthropathy, and recombinant immune-modulating drugs for ra treatment are under development. we aimed to study the putative immune regulation of rsjcystatin and its prophylactic/therapeutic effects on murine collagen-induced arthritis (cia). cia was induced ...201627393379
mitochondrial gene sequences among different geographical isolates of schistosoma japonicum in yunnan province, china.in order to evaluate differentiate genetic differences among schistosoma japonicum isolates from dali ancient city, xizhou and yongsheng county, yunnan province, china, mitochondrial col, cytb, nd1, nd6, and nd4l were pcr amplified and sequenced, revealing nucleotide difference(s) among these strains of 8, 1, 5, 4, and 0, respectively. phylogenetic analysis showed that s. japonicum from the three different geographical locations of yunnan province were clustered genetically together and were mor ...201627405120
corilagin ameliorates schistosomiasis hepatic fibrosis through regulating il-13 associated signal pathway in vitro and in vivo.interleukin (il)-13-associated signal pathway plays an important role in schistosomiasis hepatic fibrosis. in this study we tried to investigate the effects of corilagin to ameliorate schistosomiasis hepatic fibrosis through regulating il-13-associated signal pathway in vitro and in vivo. cellular model was set up with hepatic stellate cells-t6 cells stimulated by ril-13 and male balb/c mice were infected with schistosoma japonicum cercariaeas as animal model. liver histological changes were obs ...201627439782
identification of host insulin binding sites on schistosoma japonicum insulin receptors.schistosoma japonicum insulin receptors (sjirs) have been identified as encouraging vaccine candidates. interrupting or blocking the binding between host insulin and the schistosome insulin receptors (irs) may result in reduced glucose uptake leading to starvation and stunting of worms with a reduction in egg output. to further understand how schistosomes are able to exploit host insulin for development and growth, and whether these parasites and their mammalian hosts compete for the same insuli ...201627441998
il-33 contributes to schistosoma japonicum-induced hepatic pathology through induction of m2 macrophages.interleukin (il)-33 is involved in t helper (th)2-biased immune responses in mice infected with schistosoma, but the precise mechanism remains to be elucidated. herein, we investigated the role of il-33 and its receptor st2l in hepatic granuloma pathology induced by schistosoma japonicum infection. we found that il-33 induced the increased production of il-5 and il-13 from splenocytes and liver mononuclear cells (mncs) of infected mice. the infected mice developed significantly higher number of ...201627445267
antischistosomal activity of hederacochiside c against schistosoma japonicum harbored in experimentally infected animals.the present study was undertaken to investigate whether hederacochiside c (hsc) possesses antischistosomal effects and anti-inflammatory response activities in schistosoma japonicum-infected mice. different concentrations of hsc were administrated to the mice infected by schistosomula or adult worm by intravenous injection twice a day for five consecutive days. the total worm burden, female worm burden, and the egg burden in liver of mice treated with 400 mg/kg hsc were fewer than those in non-t ...201727454107
assessment of the effect of treatment and assistance program on advanced patients with schistosomiasis japonica in china from 2009 to 2014.schistosomiasis is one of the most important zoonoses, threatening approximately 800 million people in 78 countries with a loss of 70 million disability-adjusted life years. over the past six decades, china has made remarkable achievements in morbidity control, but disability and mortality control remains much to desire; thus, advanced schistosomiasis is a growing problem when on the road to schistosomiasis elimination. since 2005, china has initiated a national treatment and assistance program ...201627461114
comparison of apoptosis between adult worms of schistosoma japonicum from susceptible (balb/c mice) and less-susceptible (wistar rats) hosts.schistosomiasis remains a serious public health concern in china. balb/c mice are susceptible to schistosoma japonicum infection, whereas the wistar rats are less susceptible. apoptosis phenomenon was observed in 42d adult worms of s. japonicum from both rats and mice at the morphologic, dna, cellular, and gene levels by transmission electron microscopy (tem), fluorometric terminal deoxynucleotidyl transferase-mediated dutp nick-end labeling (tunel) analysis, fluorescein isothiocyanate-annexin-v ...201627461946
egg antigen p40 of schistosoma japonicum promotes senescence in activated hepatic stellate cells by activation of the stat3/p53/p21 pathway.liver fibrosis is a serious disease that is characterized by the excess deposition of extracellular matrix (ecm) components. activated hepatic stellate cells (hscs) are a major source of ecm and serve as a key regulator in liver fibrogenesis. inactivation of hscs is essential for liver fibrotic regression. the present study explores the underlying mechanisms of schistosoma japonicum egg antigen p40 (sjp40) promoting senescence in hscs and antifibrosis. for the first time we report that sjp40 inh ...201627468691
new detection method in experimental mice for schistosomiasis: clinprotool and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.oncomelania hupensis snails along the yangtze river and the low positive rate and infectiosity of human and livestock schistosomiasis still pose a threat to public health in china. adult blood flukes were recognized as schistosoma japonicum, which are found in the portal system of the sentinel mice bred in the laboratory for 35 days after contact with the water. however, 35 days was too long from the field test to dissection, and the dissection in the laboratory was also time-consuming and labor ...201627469535
soluble egg antigens of schistosoma japonicum induce senescence in activated hepatic stellate cells by activation of the stat3/p53/p21 pathway.liver fibrosis is characterized by the activation of hepatic stellate cells (hscs). recent findings suggest that senescence of activated hscs might limit the development of liver fibrosis. based on previously observed anti-fibrotic effects of soluble egg antigens from schistosoma japonicum in vitro, we hypothesized that sea might play a crucial role in alleviating liver fibrosis through promoting senescence of activated hscs. we show here that sea inhibited expression of α-sma and pro-collagen i ...201627489164
proteome-wide analysis of lysine acetylation in adult schistosoma japonicum worm.lysine acetylation, a ubiquitous and conserved posttranslational modification, has recently been shown to participate in many diverse non-chromatin-associated biological processes in prokaryotes and eukaryotes. however, the full extent and functional significance of acetylation in schistosoma japonicum is still unknown. to investigate the nature, extent, and biological functions of lysine acetylation in schistosomes, immunoaffinity-based acetyl-lysine peptide enrichment, integrated with mass spe ...201627535354
recombinase polymerase amplification combined with a lateral flow dipstick for rapid and visual detection of schistosoma japonicum.with the continuous decline in prevalence and intensity of schistosoma japonicum infection in china, more accurate and sensitive methods suitable for field detection become much needed for schistosomiasis control. here, a novel rapid and visual detection method based on the combination of recombinase polymerase amplification (rpa) and lateral flow dipstick (lfd) was developed to detect s. japonicum dna in fecal samples.201627577576
recombinant sj16 from schistosoma japonicum contains a functional n-terminal nuclear localization signal necessary for nuclear translocation in dendritic cells and interleukin-10 production.sj16 is a schistosoma japonicum-derived protein (16 kda in molecular weight) that has been identified as an immune modulation molecule, but the mechanisms of modulation of immune responses are not known. in this report, we aimed to investigate the host immune regulation mechanism by recombinant sj16 (rsj16) and thus illuminate the molecular mechanism of immune evasion by s. japonicum. the effect of rsj16 and rsj16 mutants on the biology of dendritic cells (dcs) was assessed by examining dc matur ...201627640151
inhibition of cytokine response to tlr stimulation and alleviation of collagen-induced arthritis in mice by schistosoma japonicum peptide sjmhe1.helminth-derived products have recently been shown to prevent the development of inflammatory diseases in mouse models. however, most identified immunomodulators from helminthes are mixtures or macromolecules with potentially immunogenic side effects. we previously identified an immunomodulatory peptide called sjmhe1 from the hsp60 protein of schistosoma japonicum. in this study, we assessed the ability of sjmhe1 to affect murine splenocytes and human peripheral blood mononuclear cells (pbmcs) s ...201727677654
history of schistosomiasis epidemiology, current status, and challenges in china: on the road to schistosomiasis elimination.schistosomiasis is a snail-borne disease caused by worms of the genus schistosoma. worldwide, human schistosomiasis remains a serious public health problem, threatening ∼800 million people in 78 countries with a loss of 70 million disability-adjusted life years. schistosoma japonicum is the only human blood fluke that occurs in china. as one of the countries suffering greatly from schistosomiasis, over the past 65 years, china has made great strides in controlling schistosomiasis, blocking the t ...201627679451
ascending colon cancer associated with deposited ova of schistosoma japonicum in non-endemic area.some reports suggest the positive correlation between schistosoma japonicum infection and colorectal cancer, however the sufficient evidence that supports a causal relationship between them has not been established. japan used be an endemic area of s. japonicum infection for 40 years ago. but now all of japan is a non-endemic area of s. japonicum infection. we report a case of ascending colon cancer associated with deposited ova of s. japonicum in non-endemic area.201627713858
schistosomiasis vaccines: where do we stand?schistosomiasis, caused mainly by s. mansoni, s. haematobium and s. japonicum, continues to be a serious tropical disease and public health problem resulting in an unacceptably high level of morbidity in countries where it is endemic. praziquantel, the only drug currently available for treatment, is unable to kill developing schistosomes, it does not prevent re-infection and its continued extensive use may result in the future emergence of drug-resistant parasites. this scenario provides impetus ...201627716365
a spatial analysis of human schistosoma japonicum infections in hubei, china, during 2009-2014.the province of hubei is located in the middle of china, near the middle and lower reaches of the river yangtze, and is an area where schistosomiasis is endemic. it is challenging to control this disease in this environment, and it would be useful to identify clusters of infection and transmission, as well as their distributions during recent years. therefore, this study aimed to analyze the spatial distribution of schistosomiasis in hubei, in order to facilitate the effective control and elimin ...201627716421
risk factors for human helminthiases in rural philippines.a cross-sectional survey was performed in 2012 among 18 rural barangays in northern samar, the philippines in order to determine the prevalence of single and multiple species helminth infections and the underlying risk factors of acquiring one or more parasites.201727717859
cloning and in vitro characterization of a schistosoma japonicum aquaglyceroporin that functions in osmoregulation.as one of the three major human pathogens that cause schistosomiasis, schistosoma japonicum is the only one that is endemic in china. despite great progress on schistosomiasis control over the past 50 years in china, s. japonicum transmission still occurs in certain endemic regions, which causes significant public health problems and enormous economic losses. during different life stages, parasites are able to survive dramatic osmolality changes between its vector, fresh water, and mammal host. ...201627733755
non-equilibrium plasma prevention of schistosoma japonicum transmission.schistosoma japonicum is a widespread human and animal parasite that causes intestinal and hepatosplenic schistosomiasis linked to colon, liver and bladder cancers, and anemia. estimated 230 million people are currently infected with schistosoma spp, with 779 million people at risk of contracting the parasite. infection occurs when a host comes into contact with cercariae, a planktonic larval stage of the parasite, and can be prevented by inactivating the larvae, commonly by chemical treatment. ...201627739459
biennial versus annual treatment for schistosomiasis and its impact on liver morbidity.this study assessed the impact of annual versus biennial praziquantel treatment regimens on the prevalence, intensity of infection, and liver fibrosis dynamics of asiatic schistosomiasis (caused by schistosoma japonicum) among individuals residing in 18 endemic barangays in northern samar, philippines.201727743969
micror-146 blocks the activation of m1 macrophage by targeting signal transducer and activator of transcription 1 in hepatic schistosomiasis.schistosomiasis is a chronic disease caused by the parasite of the schistosoma genus and is characterized by egg-induced hepatic granulomas and fibrosis. macrophages play a central role in schistosomiasis with several studies highlighting their differentiation into m2 cells involved in the survival of infected mice through limitation of immunopathology. however, little is known regarding the mechanisms of regulating macrophage differentiation. here, we showed that the early stage of infection by ...201627780686
blockade of pd-1 signaling enhances th2 cell responses and aggravates liver immunopathology in mice with schistosomiasis japonica.more than 220 million people worldwide are chronically infected with schistosomes, causing severe disease or even death. the major pathological damage occurring in schistosomiasis is attributable to the granulomatous inflammatory response and liver fibrosis induced by schistosome eggs. the inflammatory response is tightly controlled and parallels immunosuppressive regulation, constantly maintaining immune homeostasis and limiting excessive immunopathologic damage in important host organs. it is ...201627792733
development of an immunodiagnosis method using recombinant pscp for detection of paragonimus skrjabini infection in human.paragonimiasis skrjabini is a kind of zoonosis and prevalent in 16 provinces in china, such as chongqing, fujian, sichuan, and yunnan. however, sensitive and efficient diagnostic methods for the infection are limited. in order to provide a more convenient and simple method for serologic diagnosis, the recombinant p. skrjabini cysteine protease (pscp) was expressed, purified, and then used to develop an indirect enzyme-linked immunosorbent assay (elisa) for detecting anti-pscp antibodies in human ...201727796563
vascular endothelial growth factor promotes the activation of hepatic stellate cells in chronic schistosomiasis.the activation of hepatic stellate cells (hscs) is a key event in fibrotic pathogenesis. however, the mechanism involving activation of hscs in chronic schistosomiasis is not entirely clear. human hsc lx-2 and human umbilical vein endothelial cells (ecs) were cultured with schistosoma japonicum antigens (sa) in vitro. fibrosis-associated genes and cell proliferation were analyzed. hscs were isolated from mice of chronic schistosomiasis with or without praziquantel (pzq) treatment, followed by th ...201727808086
long-term effectiveness of the integrated schistosomiasis control strategy with emphasis on infectious source control in china: a 10-year evaluation from 2005 to 2014.schistosomiasis is a neglected tropical parasitic disease of great public health significance worldwide. currently, mass drug administration with praziquantel remains the major strategy for global schistosomiasis control programs. since 2005, an integrated strategy with emphasis on infectious source control was implemented for the control of schistosomiasis japonica, a major public health concern in china, and pilot studies have demonstrated that such a strategy is effective to reduce the preval ...201727812902
release of extracellular vesicles containing small rnas from the eggs of schistosoma japonicum.schistosoma japonicum is a parasitic flatworm that causes human schistosomiasis. secreted extracellular vesicles (evs) play a key role in pathogen-host interfaces. previous studies have shown that s. japonicum adult worms can release microrna (mirna)-containing evs, which can transfer their cargo to mammalian cells and regulate gene expression in recipient cells. tissue-trapped eggs are generally considered the major contributor to the severe pathology of schistosomiasis; however, the interactio ...201627825390
genetic diversity and structure of schistosoma japonicum within two marshland villages of anhui, china, prior to schistosome transmission control and elimination.schistosomiasis is caused by the genus schistosoma and affected more than 250 million people worldwide. schistosoma japonicum was once seriously endemic in china and nearly 60 years of efforts has seen great success in disease control. however, due to its zoonotic nature and complex life cycle, the schistosomiasis transmission control and final elimination would require, besides an intersectoral approach, deep understanding of population genetics of the parasite. we therefore performed a snail s ...201727838835
mechanism of corilagin interference with il-13/stat6 signaling pathways in hepatic alternative activation macrophages in schistosomiasis-induced liver fibrosis in mouse model.this study tried to find the mechanism of corilagin interference with interleukin (il)-13/signal transducer and activator of transcription (stat) 6 signaling pathways in il-13-activated liver alternative activation macrophages in schistosomiasis-induced liver fibrosis in balb/c mice. as a result, il-13 in serum and the mrna expression of il-13 receptor α1, il-4 receptor α and downstream mediators supressor of cytokine signaling (socs) 1, kruppel-like factor (klf) 4, peroxisome proliferator-activ ...201627845069
ancient human parasites in ethnic chinese populations.whilst archaeological evidence for many aspects of life in ancient china is well studied, there has been much less interest in ancient infectious diseases, such as intestinal parasites in past chinese populations. here, we bring together evidence from mummies, ancient latrines, and pelvic soil from burials, dating from the neolithic period to the qing dynasty, in order to better understand the health of the past inhabitants of china and the diseases endemic in the region. seven species of intest ...201627853113
sm-p80-based schistosomiasis vaccine: preparation for human clinical trials.mass antiparasitic drug administration programs and other control strategies have made important contributions in reducing the global prevalence of helminths. schistosomiasis, however, continues to spread to new geographic areas. the advent of a viable vaccine and its deployment, coupled with existing control efforts, is expected to make significant headway towards sustained schistosomiasis control. in 2016, science ranked the schistosomiasis vaccine as one of the top 10 vaccines that needs to b ...201727865740
the spatio-temporal distribution of oncomelania hupensis along yangtze river in jiangsu province, china after implementation of a new, integrated schistosomiasis control strategy.this study concerns an integrated strategy of schistosomiasis control, focusing on the management and elimination of the main transmission cycles and reservoirs along yangtze river, jiangsu province (china), instituted in 2004. our analysis, including mapping, spatial autocorrelation and spatio-temporal scanning, was implemented between 2001 and 2013 to explore the changes in the distribution of oncomelania hupensis, the intermediate host of schistosoma japonicum. two high-density snail location ...201627903066
differential pulmonic nk and nkt cell responses in schistosoma japonicum-infected mice.natural killer cells (nk cells) and natural killer t cells (nkt cells) play a role in anti-infection, anti-tumor, transplantation immunity, and autoimmune regulation. however, the role of nk and nkt cells during schistosoma japonicum (s. japonicum) infection has not been widely reported, especially regarding lung infections. the aim of this study was to research the nk and nkt cell response to s. japonicum infection in the lungs of mice. using immunofluorescent histological analysis, nk and nkt ...201727904959
genome-wide mirnas expression profiles of schistosoma japonicum schistosomula in response to artesunate.mirnas play a significant role in pharmacogenomics and are likely to be important in the molecular mechanism of atesunate (art) effects on schistosoma japonicum.201627918238
evaluation of lethal giant larvae as a schistosomiasis vaccine candidate.schistosomiasis is a neglected tropical disease of humans, and it is considered to be the second most devastating parasitic disease after malaria. eggs produced by normally developed female worms are important in the transmission of the parasite, and they responsible for the pathogenesis of schistosomiasis. the tumor suppressor gene lethal giant larvae (lgl) has an essential function in establishing apical-basal cell polarity, cell proliferation, differentiation, and tissue organization. in our ...201627957496
genetic evidence of contemporary dispersal of the intermediate snail host of schistosoma japonicum: movement of an ntd host is facilitated by land use and landscape connectivity.while the dispersal of hosts and vectors-through active or passive movement-is known to facilitate the spread and re-emergence of certain infectious diseases, little is known about the movement ecology of oncomelania spp., intermediate snail host of the parasite schistosoma japonicum, and its consequences for the spread of schistosomiasis in east and southeast asia. in china, despite intense control programs aimed at preventing schistosomiasis transmission, there is evidence in recent years of r ...201627977674
soluble egg antigens of schistosoma japonicum induce senescence of activated hepatic stellate cells by activation of the foxo3a/skp2/p27 pathway.liver fibrosis was viewed as a reversible process. the activation of hepatic stellate cells (hscs) is a key event in the process of liver fibrosis. the induction of senescence of hscs would accelerate the clearance of the activated hscs. previously, we demonstrated that soluble egg antigens (sea) of schistosoma japonicum promoted the senescence of hscs via stat3/p53/p21 pathway. in this paper, our study was aimed to explore whether there are other signaling pathways in the process of sea-induced ...201628036393
erratum: il-33 contributes to schistosoma japonicum-induced hepatic pathology through induction of m2 macrophages. 201728051120
cysteine protease inhibitor of schistosoma japonicum - a parasite-derived negative immunoregulatory factor.studies have shown that cysteine protease inhibitors from some parasites have immunosuppressive effects on the host. we previously have cloned a novel cysteine protease inhibitor from schistosoma japonicum and purified its recombinant version (protein named rsj-c). its possible inhibitory effect on the host immune response has not been described.this study shows that rsj-c inhibits lysosomal cysteine protease of murine dendritic cells (dcs). after dcs were incubated with rsj-c and then with solu ...201728066871
a next-generation microarray further reveals stage-enriched gene expression pattern in the blood fluke schistosoma japonicum.schistosomiasis is caused by infection with blood flukes of the genus schistosoma, and ranks, in terms of disability-adjusted life years (dalys), as the third most important neglected tropical disease. schistosomes have several discrete life stages involving dramatic morphological changes during their development, which require subtle gene expression modulations to complete the complex life-cycle.201728069074
whole genome amplification and reduced-representation genome sequencing of schistosoma japonicum miracidia.in areas where schistosomiasis control programs have been implemented, morbidity and prevalence have been greatly reduced. however, to sustain these reductions and move towards interruption of transmission, new tools for disease surveillance are needed. genomic methods have the potential to help trace the sources of new infections, and allow us to monitor drug resistance. large-scale genotyping efforts for schistosome species have been hindered by cost, limited numbers of established target loci ...201728107347
gender-based differences in water, sanitation and hygiene-related diarrheal disease and helminthic infections: a systematic review and meta-analysis.qualitative evidence suggests that inadequate water, sanitation and hygiene (wash) may affect diarrheal and helminthic infection in women disproportionately. we systematically searched pubmed in june 2014 (updated 2016) and the who website, for relevant articles.201728115686
a loop-mediated isothermal amplification (lamp) assay targeting 16s rrna gene for rapid detection of anaplasma phagocytophilum infection in sheep and goats.anaplasma phagocytophilum is a zoonotic pathogen and the causative agent of human granulocytic anaplasmosis (hga) in humans and tick-borne fever in various kinds of animals. in the present study, a loop-mediated isothermal amplification (lamp) assay for rapid detection of a. phagocytophilum was developed using primers specific to 16s rrna gene of this organism. the lamp assay was performed at 65 c for 60 min and terminated at 80 c for 10 min. the optimal reaction conditions, under which no cross ...201728118097
specific anti-glycan antibodies are sustained during and after parasite clearance in schistosoma japonicum-infected rhesus macaques.human immunity to schistosoma infection requires many years of exposure, and multiple infections and treatments to develop. unlike humans, rhesus macaques clear an established schistosome infection naturally at the same time acquiring immunity towards re-infection. in macaques, schistosome egg production decreases after 8 weeks post-infection and by week 22, physiological impairment of the worm caused by unclarified antibody-mediated processes is observed. since strong antibody responses have be ...201728151933
a field trial of recombinant schistosoma japonicum paramyosin as a potential vaccine in naturally-infected water buffaloesthe overall aims of this project are to assess the safety and immunogenicity of the schistosoma japonicum vaccine paramyosin among water buffaloes residing in endemic areas. the study was conducted in four villages in leyte, the philippines, an area highly endemic for schistosomiasis japonica. one hundred and fifteen (n=115) animals provided baseline stool samples for coprologic examination, with preliminary results using flotac showing a 10% prevalence of schistosomiasis. forty-nine (n=49) anim ...201628158940
genistein improves schistosomiasis liver granuloma and fibrosis via dampening nf-kb signaling in mice.in schistosomiasis, egg deposition in the liver contributes to the formation of hepatic granuloma and fibrosis, which are the most serious clinical pathological features. it has been proposed that activation of the nuclear factor kappa b (nf-κb) signaling pathways is closely associated with the development of hepatic granuloma and fibrosis. genistein has been shown to inhibit the activity of nf-κb signaling pathways, which might be a potential agent to protect against schistosoma japonicum egg-i ...201728160073
changes in microrna expression in response to schistosoma japonicum infection.schistosomiasis japonicum is one of the most serious zoonotic diseases in the world. there is increasing evidence to show that host mirnas are modulated following schistosoma japonicum infection, and some of these mirnas may play important regulatory roles in response to schistosome infection. several host mirnas have been identified and shown to be potential diagnostic biomarkers or novel therapeutic targets for schistosomiasis. these studies have paved the way to a better understanding of the ...201728160510
the dynamic changes of cd3e(-)cd11c(+) dendritic cells in spleens and bone marrow of mice infected with schistosoma japonicum.schistosoma japonicum as a pathogeny requires dendritic cells to activate immune response. so, the research is to study the dynamic changes of cd3e(-)cd11c(+) dendritic cells in mice infected with s. japonicum. zero, 7, 28, 35, and 63 days were selected to study the variation of dendritic cells, and the proportions of cd3e(-)cd11c(+) dendritic cells and cd86(+) mature dendritic cells in spleens and bone marrow were tested by flow cytometry. as a result, the variation trends of dendritic cells in ...201728185057
high-throughput dynamic analysis of differentially expressed genes in splenic dendritic cells from mice infected with schistosoma japonicum.dendritic cells are the initiation and key point of immune response and play a role in immune regulation. so we explored the mechanisms involved in immune regulation of dendritic cells (dcs) against schistosomiasis using mice infected with schistosoma japonicum. splenic dcs from normal mice and mice with acute and chronic s. japonicum infection were sorted by flow cytometry. the numbers and functions of differentially expressed genes (degs) in dcs were determined by high-throughput analysis. all ...201728185924
the role of st2 and st2 genetic variants in schistosomiasis.chronic schistosomiasis and its severe complication, periportal fibrosis, are characterized by a predominant th2 response. to date, specific single nucleotide polymorphisms in st2 have been some of the most consistently associated genetic variants for asthma.201728189770
characterization of schistosoma japonicum cp1412 protein as a novel member of the ribonuclease t2 molecule family with immune regulatory function.schistosome infection typically induces a polarized th2 type host immune response. as egg antigen molecules play key roles in this immunoregulatory process, clarifying their functions in schistosomiasis would facilitate the development of vaccine and immunotherapeutic methods. schistosoma japonicum (sj) cp1412 (genbank: ay57074.1) has been identified as a new member of the rnase t2 family with immune regulatory functions.201728212670
genetic diversity and selection of three nuclear genes in schistosoma japonicum populations.the blood fluke, schistosoma japonicum still causes severe disease in china, the philippines and indonesia. although there have been some studies the molecular epidemiology of this persistent and harmful parasite, few have explored the possibility and implications of selection in s. japonicum populations.201728212676
acute appendicitis caused by schistosoma japonicum. 201728216022
field evaluation of a recombinase polymerase amplification assay for the diagnosis of schistosoma japonicum infection in hunan province of china.current diagnostic methods for schistosoma japonicum infection are insensitive for low-density infections. therefore, a new diagnostic assay based on recombinase polymerase amplification (rpa) technology was established and assessed for field applification.201728222680
evaluation of protective efficacy induced by different heterologous prime-boost strategies encoding triosephosphate isomerase against schistosoma japonicum in mice.in china, schistosomiasis japonica is a predominant zoonotic disease, and animal reservoir hosts in the environment largely sustain infections. the development of transmission-blocking veterinary vaccines is urgently needed for the prevention and efficient control of schistosomiasis. heterologous prime-boost strategy is more effective than traditional vaccination and homologous prime-boost strategies against multiple pathogens infection. in the present study, to further improve protective effica ...201728241779
early detection of circulating dna of schistosoma japonicum in sentinel mice models.currently in china, the schistosomiasis control program has shifted its focus from transmission control to the elimination of the disease. effective forecast and surveillance systems of schistiosomiasis are of great importance for issuing timely and early warnings on risk of infection, and therefore implementing preventive measures to avoid infection. there is great demand in more sensitive and specific methods to improve the surveillance system for early detection of s. japonicum infection in s ...201728268185
apropos: critical analysis of molluscicide application in schistosomiasis control programs in brazil.schistosomiasis is a snail-transmitted infectious disease affecting over 200 million people worldwide. snail control has been recognized as an effective approach to interrupt the transmission of schistosomiasis, since the geographic distribution of this neglected tropical disease is determined by the presence of the intermediate host snails. in a recent scoping review published in infectious diseases of poverty, coelho and caldeira performed a critical review of using molluscicides in the nation ...201728270227
a novel duplex ddpcr assay for the diagnosis of schistosomiasis japonica: proof of concept in an experimental mouse model.the current world health organization strategic plan targets the elimination of schistosomiasis as a public health problem by 2025 and accurate diagnostics will play a pivotal role in achieving this goal. dna-based detection methods provide a viable alternative to some of the commonly used tests, notably microscopy and serology, for the diagnosis of schistosomiasis. the detection of parasite cell-free dna in different clinical samples is a recent valuable advance, which provides significant bene ...201728274280
biochemical properties and vaccine effect of recombinant tpx-3 from schistosoma japonicum.thioredoxin peroxidases (tpxs) play an important role in maintaining redox homeostasis and in protecting organisms from the accumulation of toxic reactive oxygen species (ros). in this study, we isolated the thioredoxin peroxidase-3 gene of schistosoma japonicum, sjtpx-3. the open reading frame (orf) of sjtpx-3 was 663 bp encoding 220 amino acids with a molecular weight of 24.99 kda and an isoelectric point of 6.20. quantitative real-time reverse transcription-polymerase chain reaction indicated ...201728285327
dynamic transcriptomes identify biogenic amines and insect-like hormonal regulation for mediating reproduction in schistosoma japonicum.eggs produced by the mature female parasite are responsible for the pathogenesis and transmission of schistosomiasis. female schistosomes rely on a unique male-induced strategy to accomplish reproductive development, a process that is incompletely understood. here we map detailed transcriptomic profiles of male and female schistosoma japonicum across eight time points throughout the sexual developmental process from pairing to maturation. the dynamic gene expression pattern data reveal clear sex ...201728287085
a multidisciplinary, integrated approach for the elimination of schistosomiasis: a longitudinal study in a historically hyper-endemic region in the lower reaches of the yangtze river, china from 2005 to 2014.although great success has been achieved, schistosomiasis remains a major public health concern in china, and the remaining core endemic regions are concentrated along the middle and lower reaches of the yangtze river. in this longitudinal study, we evaluated the effectiveness of a multidisciplinary, integrated approach for schistosomiasis elimination in a historically hyper-endemic region in the lower reaches of the yangtze river, china over the 10-year period from 2005 through 2014.201728288689
approaches being used in the national schistosomiasis elimination programme in china: a review.schistosomiasis japonica, caused by the human blood fluke schistosoma japonicum, remains a major public health problem in china, although great success has been achieved. the control efforts during the past half-decade, notably the wide implementation of the new integrated strategy with emphasis on control of the source of s. japonicum infection across the country since 2004, has greatly reduced s. japonicum in humans, livestock, and intermediate host oncomelania hupensis snails, and transmissio ...201728292327
identification and functional characterization of oncomelania hupensis macrophage migration inhibitory factor involved in the snail host innate immune response to the parasite schistosoma japonicum.schistosomiasis, caused by parasitic trematodes of the genus schistosoma, remains a devastating public health problem, with over 200 million people infected and 779 million people at risk worldwide, especially in developing countries. the freshwater amphibious snail oncomelania hupensis is the obligate intermediate host of schistosoma japonicum. this unique and long-standing host-parasite interaction highlights the biomedical importance of the molecular and cellular mechanisms involved in the sn ...201728322847
egg antigen p40 of schistosoma japonicum promotes senescence in activated hepatic stellate cells via skp2/p27 signaling pathway.schistosomiasis is characterized by egg deposition, granulomatous inflammatory reaction and then subsequent hepatic fibrosis formation. activated hscs are regarded as the main effector cells in the progression of liver fibrosis and induction of senescence in hepatic stellate cells (hscs) is vital to the reversion of hepatic fibrosis. our previous work has showed that s. japonicum egg antigen p40 (sjp40) could promote hscs senescence via a stat3/p53/p21 mechanism. in this paper, the major aim was ...201728325896
in vivo and in vitro activity of oil extract of garlic (allium sativum linnaeus) against schistosoma japonicum cercariae.the activity of garlic oil extract against schistosoma japonicum cercariae was evaluated.201728327815
an integrated environmental improvement of marshlands: impact on control and elimination of schistosomiasis in marshland regions along the yangtze river, china.schistosomiasis is a global snail-transmitted infectious disease of poverty. transmission control had been achieved in china in 2015 after the control efforts for over 60 years. currently, the remaining core regions endemic for schistosoma japonicum are mainly located in the marshland and lake regions along the yangtze river basin.201728330510
enhanced wnt signalling in hepatocytes is associated with schistosoma japonicum infection and contributes to liver fibrosis.liver fibrosis is the most serious pathology caused by schistosoma japonicum infection, which arises when schistosome eggs are deposited in the liver. eosinophils, macrophages and hepatic stellate cells (hscs) have been identified as major cellular contributors to the development of granulomas and fibrosis, but little is known about the effects of hepatocytes on granuloma formation. here, we found that the levels of wnt signalling-related molecules, transforming growth factor β (tgf-β) and conne ...201728331224
formation and controlled drug release using a three-component supramolecular hydrogel for anti-schistosoma japonicum cercariae.a novel three-component supramolecular hydrogel based on riboflavin, melamine and amino acid derivatives were constructed for controlled release of pesticides, niclosamide derivatives. the formation of hydrogel may be attributed to self-assemble via hydrogen bonding and π-π interaction, which have been researched via scanning electron microscopy (sem) and fourier transform infrared (ft-ir) spectra. the rheological experiments showed that the hydrogel materials and drug-loaded hydrogel all demons ...201628344302
a rapid, simple and sensitive loop-mediated isothermal amplification method to detect anaplasma bovis in sheep and goats samples.a loop-mediated isothermal amplification (lamp) technique has been widely used in detecting the nucleic acid of various pathogenic bacteria. in this study, a set of four lamp primers was designed to specifically test anaplasma bovis. the lamp assay was performed at 62°c for 60min in a water bath. the specificity was confirmed by amplifying a. bovis isolate, while no cross reaction was observed with other five pathogens (anaplasma bovis, anaplasma phagocytophilum, theileria luwenshuni, babesia mo ...201728351721
function of nanos1 gene in the development of reproductive organs of schistosoma japonicum.nanos is a necessary factor in the differentiation and migration of primordial germ cells. it is closely associated with the development of genitalia in a wide range of species. we questioned whether nanos was involved in the reproductive organ development of schistosoma japonicum. firstly, by in situ hybridization, s. japonicum nanos1 (sjnanos1) gene was expressed mainly in reproductive organs of s. japonicum. then, the paired schistosome of 28 days post-infection (dpi) was transfected with ...201728352943
clinical diagnostic value of viable schistosoma japonicum eggs detected in host tissues.schistosomiasis, one of the neglected tropical diseases, is endemic in more than 70 countries. however, the clinical diagnosis of patients with a low degree of infection is an unsolved technical problem. in areas endemic for schistosomiasis japonica, proctoscopy detection of eggs has been one method used for clinical diagnosis. however, it is often a challenge to find typical live eggs and it is difficult to distinguish live eggs from large numbers of partially degraded and/or completely degrade ...201728376858
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