Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| enteropathogenic escherichia coli infection induces expression of the early growth response factor by activating mitogen-activated protein kinase cascades in epithelial cells. | enteropathogenic escherichia coli (epec) is an extracellular bacterial pathogen that infects the human intestinal epithelium and is a major cause of infantile diarrhea in developing countries. epec belongs to the group of attaching and effacing (a/e) pathogens. it uses a type iii secretion system to deliver proteins into the host cell that mediate signal transduction events in host cells. we used gene array technology to study epithelial cell responses to epec infection at the level of gene expr ... | 2001 | 11553563 |
| use of operon fusions in mannheimia haemolytica to identify environmental and cis-acting regulators of leukotoxin transcription. | the leukotoxin of mannheimia haemolytica is an important virulence factor that contributes to much of the pathology observed in the lungs of animals with bovine shipping fever pneumonia. we believe that identification of factors that regulate leukotoxin expression may provide insight into m. haemolytica pathogenicity. the dna sequence upstream of the leukotoxin operon is divergently shared by p(lapt), which transcribes an arginine permease gene. the intergenic region contains several elements th ... | 2001 | 11553565 |
| mimicry of a g protein mutation by pertussis toxin expression in transgenic caenorhabditis elegans. | pathogens produce virulence factors that interact directly with host molecules, but in many cases the host targets are unknown. the genetic and molecular identification of these orphan targets is often not feasible with mammalian experimental models. however, a substantial number of known targets are molecules and pathways that are conserved among eukaryotes, and therefore the use of nonmammalian model hosts to identify orphan targets may prove useful. to demonstrate the feasibility of this appr ... | 2001 | 11553570 |
| epidemic population structure of pseudomonas aeruginosa: evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors. | the genetic structure of a population of pseudomonas aeruginosa, isolated from patients with keratitis, endophthalmitis, and contact lens-associated red eye, contact lens storage cases, urine, ear, blood, lungs, wounds, feces, and the environment was determined by multilocus enzyme electrophoresis. the presence and characteristics of virulence factors were determined by restriction fragment length polymorphism analysis with dna probes for lasa, lasb, apra, exos, exot, exou, and ctx and by zymogr ... | 2001 | 11553572 |
| gonococcal genes encoding transferrin-binding proteins a and b are arranged in a bicistronic operon but are subject to differential expression. | neisseria gonorrhoeae is capable of utilizing host iron-binding proteins, such as transferrin, lactoferrin, and hemoglobin, as the sole source of iron. the receptor involved in transferrin iron acquisition is composed of two distinct transferrin-binding proteins, tbpa and tbpb. the genes that encode these proteins are linked on the chromosome in the order tbpb-tbpa but are separated by an inverted repeat of unknown function. in this study, we sought to understand the transcriptional organization ... | 2001 | 11553578 |
| intracellular induction of the bartonella henselae virb operon by human endothelial cells. | one of the more recently identified bacterial exportation systems is the type iv secretion mechanism, which is characterized by a multiprotein complex that spans the inner and outer bacterial membranes and contains a pilin component. the most thoroughly studied type iv secretion system is encoded by the virb operon of agrobacterium tumefaciens. in bartonella henselae, 8 of the 10 virb operon genes share extensive homology and arrangement with the virb operon of a. tumefaciens. sequencing of the ... | 2001 | 11553594 |
| purification of anthrax edema factor from escherichia coli and identification of residues required for binding to anthrax protective antigen. | the structural gene for anthrax edema factor (ef) was expressed in escherichia coli under the control of a powerful t5 promoter to yield the 89-kda recombinant protein that reacted with anti-ef antibodies. recombinant ef was purified to homogeneity by a two-step procedure involving metal chelate affinity chromatography and cation-exchange chromatography. from 1 liter of culture, 2.5 mg of biologically active ef was easily purified. this is the first report of purification of anthrax ef from e. c ... | 2001 | 11553601 |
| novel domains of the prokaryotic two-component signal transduction systems. | the archetypal two-component signal transduction systems include a sensor histidine kinase and a response regulator, which consists of a receiver chey-like domain and a dna-binding domain. sequence analysis of the sensor kinases and response regulators encoded in complete bacterial and archaeal genomes revealed complex domain architectures for many of them and allowed the identification of several novel conserved domains, such as pas, gaf, hamp, ggdef, eal, and hd-gyp. all of these domains are w ... | 2001 | 11557134 |
| [integration and intramolecular transposition of the tnbp3 bordetella pertussis transposon in the escherichia coli k-12 cells -- mutant for the phosphoenolpyruvate-dependent phosphotransferase system hpr protein ]. | integration of a plasmid carrying the tnbp3 transposon of bordetella pertussis into the chromosome of escherichia coli and transpositions of the integrated structure within a chromosome in the wild-type and mutant cells ptsh devoid of the major hpr protein of the phosphoenolpyruvate-dependent phosphotransferase system were studied. when transposed to a new chromosome site, the integrated structure was precisely (or almost precisely) excised from the mety gene sequence, which resulted in restorat ... | 2001 | 11558229 |
| monocyte selectivity and tissue localization suggests a role for breast and kidney-expressed chemokine (brak) in macrophage development. | although numerous chemokines act on monocytes, none of them is specific for these cells. here, we show that breast and kidney-expressed chemokine (brak) is a highly selective monocyte chemoattractant. migration efficacy and bordetella pertussis toxin-sensitive ca(2+) mobilization responses to brak were strongly enhanced after treatment of monocytes with the cyclic amp-elevating agents prostaglandin e(2) and forskolin. brak is the first monocyte-selective chemokine, as other types of blood leukoc ... | 2001 | 11561000 |
| laboratory diagnosis of respiratory diseases: pcr versus serology. | we present a comparison between serology and genetic detection of three bacterial pathogens causing lower respiratory tract infection (lri). we evaluated serology and pcr for the detection of mycoplasma pneumoniae, bordetella pertussis and chlamydia pneumoniae from 1712 nasopharyngeal and serum samples. for 856 nasopharyngeal samples, average pcr time was 7.2 days, the parallel serum samples was 13 days. automated extraction of nucleic acids reduces average pcr time to 6.7 days. | 2001 | 11562997 |
| subtilisin-like autotransporter serves as maturation protease in a bacterial secretion pathway. | proteins of gram-negative bacteria destined to the extracellular milieu must cross the two cellular membranes and then fold at the appropriate time and place. the synthesis of a precursor may be a strategy to maintain secretion competence while preventing aggregation or premature folding (especially for large proteins). the secretion of 230 kda filamentous haemagglutinin (fha) of bordetella pertussis requires the synthesis and the maturation of a 367 kda precursor that undergoes the proteolytic ... | 2001 | 11566869 |
| translocation-specific conformation of adenylate cyclase toxin from bordetella pertussis inhibits toxin-mediated hemolysis. | bordetella pertussis adenylate cyclase (ac) toxin belongs to the rtx family of toxins but is the only member with a known catalytic domain. the principal pathophysiologic function of ac toxin appears to be rapid production of intracellular cyclic amp (camp) by insertion of its catalytic domain into target cells (referred to as intoxication). relative to other rtx toxins, ac toxin is weakly hemolytic via a process thought to involve oligomerization of toxin molecules. monoclonal antibody (mab) 3d ... | 2001 | 11566989 |
| nonspecific adherence and fibril biogenesis by actinobacillus actinomycetemcomitans: tada protein is an atpase. | cells of actinobacillus actinomycetemcomitans, a gram-negative pathogen responsible for an aggressive form of juvenile periodontitis, form tenaciously adherent biofilms on solid surfaces. the bacteria produce long fibrils of bundled pili, which are required for adherence. mutations in flp-1, which encodes the major subunit of the pili, or any of seven downstream tad genes (tadabcdefg) cause defects in fibril production, autoaggregation, and tenacious adherence. we proposed that the tad genes spe ... | 2001 | 11566992 |
| antibodies to brka augment killing of bordetella pertussis. | brka is a bvg-regulated bordetella pertussis protein that mediates serum resistance and adherence. it shares sequence identity with another b. pertussis virulence factor called pertactin, and it is a member of the diverse group of proteins found in gram-negative bacteria that are secreted by an autotransporter mechanism. sera, either from individuals who have been vaccinated with acellular pertussis vaccines, or from individuals who have no re-collection of recent infection with b. pertussis fai ... | 2001 | 11567769 |
| functional analysis of novel multidrug transporters from human pathogens. | proteins of the smr family are the smallest multidrug transporters, about 110 amino acids long, that extrude various drugs in exchange with protons, thereby rendering bacteria resistant to these compounds. one of these proteins, emre, is an escherichia coli protein, which has been cloned based on its ability to confer resistance to ethidium and methyl viologen and which has been extensively characterized. more than 60 genes coding for smr proteins have been identified in several bacteria based o ... | 2001 | 11574548 |
| a quantitative analysis for the adp-ribosylation activity of pertussis toxin: an enzymatic-hplc coupled assay applicable to formulated whole cell and acellular pertussis vaccine products. | the majority of the biological effects of pertussis toxin (pt) are the result of a toxin-catalyzed transfer of an adenosine diphosphate-ribose (adp-ribose) moiety from nad(+)to the alpha-subunits of a subset of signal-transducing guanine-nucleotide-binding proteins (g-proteins). this generally leads to an uncoupling of the modified g-protein from the corresponding receptor and the loss of effector regulation. this assay is based on the pt s1 subunit enzymatic transfer of adp-ribose from nad to t ... | 2001 | 11580213 |
| third international standard for pertussis vaccine: international confirmation study of activity of british standard for pertussis vaccine, coded 66/303. | the first british standard (bs) (code 66/303) for pertussis whole cell vaccine was prepared from the same suspension of bordetella pertussis as the now exhausted second international standard for pertussis vaccine (2ndis). the bs and the 2ndis were compared and calibrated in a previous international study. this report describes a small international study, which included the bs, the 1stis and the 2ndis so that the present relationship of these preparations to one another could be assessed. the r ... | 2001 | 11580217 |
| [pediatric deaths due to community-acquired bacterial infection. survey of french pediatric intensive care units]. | a retrospective survey has been conducted in the pediatric intensive care units (picus) affiliated to the groupe francophone d'urgence et de réanimation pédiatrique over two years (1999 and 2000). the purpose was to determine the number of children aged from 10 days to 18 years who died from community acquired bacterial infections and to compare the data to those obtained from official surveys (statistics of death from the institut national de la santé et de la recherche médicale) and from the i ... | 2001 | 11582916 |
| [severe bacterial infections in children. survey by the pediatric mobile intensive care unit ap/hp in the ile-de-france area]. | this study involves 106 infants (neonatal period ruled out), victims of severe bacterial infections managed from 1st january 1998 to 30 april 2001 by the four paediatric mobile intensive care unit (p.m.i.c.u.) teams ap-hp in ile-de-france area. 46.2% of the whole infants are primary interventions (home, medical room, airport) and primary-secondary interventions (hospital emergencies) whereas 53.8% are related to secondary transports of infants who have been hospitalized and suffered from severe ... | 2001 | 11582917 |
| current pharmacotherapy of pertussis. | human infection with bordetella pertussis and bordetella parapertussis causes significant morbidity and mortality. while universal immunisation represents the mainstay of prevention, the purpose of this review is to summarise the current options for antimicrobial chemotherapy of pertussis. several chemotherapeutic approaches have an important place in therapy and in infection control. supportive treatment including nasopharyngeal suction, oxygen and parenteral fluids, is essential for infants < ... | 2001 | 11584995 |
| active immunization in the united states: developments over the past decade. | the centers for disease control and prevention has identified immunization as the most important public health advance of the 20th century. the purpose of this article is to review the changes that have taken place in active immunization in the united states over the past decade. since 1990, new vaccines have become available to prevent five infectious diseases: varicella, rotavirus, hepatitis a, lyme disease, and japanese encephalitis virus infection. improved vaccines have been developed to pr ... | 2001 | 11585789 |
| pseudomonas aeruginosa pao1 kills caenorhabditis elegans by cyanide poisoning. | in this report we describe experiments to investigate a simple virulence model in which pseudomonas aeruginosa pao1 rapidly paralyzes and kills the nematode caenorhabditis elegans. our results imply that hydrogen cyanide is the sole or primary toxic factor produced by p. aeruginosa that is responsible for killing of the nematode. four lines of evidence support this conclusion. first, a transposon insertion mutation in a gene encoding a subunit of hydrogen cyanide synthase (hcnc) eliminated nemat ... | 2001 | 11591663 |
| functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from pseudomonas aeruginosa pao1. | two seven-gene phenazine biosynthetic loci were cloned from pseudomonas aeruginosa pao1. the operons, designated phza1b1c1d1e1f1g1 and phza2b2c2d2e2f2g2, are homologous to previously studied phenazine biosynthetic operons from pseudomonas fluorescens and pseudomonas aureofaciens. functional studies of phenazine-nonproducing strains of fluorescent pseudomonads indicated that each of the biosynthetic operons from p. aeruginosa is sufficient for production of a single compound, phenazine-1-carboxyl ... | 2001 | 11591691 |
| the esat-6 gene cluster of mycobacterium tuberculosis and other high g+c gram-positive bacteria. | the genome of mycobacterium tuberculosis h37rv has five copies of a cluster of genes known as the esat-6 loci. these clusters contain members of the cfp-10 (lhp) and esat-6 (esat-6) gene families (encoding secreted t-cell antigens that lack detectable secretion signals) as well as genes encoding secreted, cell-wall-associated subtilisin-like serine proteases, putative abc transporters, atp-binding proteins and other membrane-associated proteins. these membrane-associated and energy-providing pro ... | 2001 | 11597336 |
| effect of attenuated salmonella enterica serovar typhimurium expressing a streptococcus mutans antigen on secondary responses to the cloned protein. | attenuated salmonella enterica serovar typhimurium has been used for targeted delivery of recombinant antigens to gut- and nose-associated lymphoid tissues. contradictory reports have described the effect of preexisting immunity to the antigen delivery vehicle. we decided to examine this discrepancy by studying the effect of immunizing mice by the intranasal (i.n.) route with salmonella expressing an insoluble protein and to study the ability to augment recall responses by boosting with either s ... | 2001 | 11598028 |
| bordetella pertussis autoregulates pertussis toxin production through the metabolism of cysteine. | pertussis toxin (ptx) expression and secretion in bordetella pertussis are regulated by a two-component signal transduction system encoded by the bvg regulatory locus. however, it is not known whether the metabolic pathways and growth state of the bacterium influence synthesis and secretion of ptx and other virulence factors. we have observed a reduction in the concentration of ptx per optical density unit midway in fermentation. studies were conducted to identify possible factors causing this r ... | 2001 | 11598055 |
| heme utilization in bordetella avium is regulated by rhui, a heme-responsive extracytoplasmic function sigma factor. | efficient utilization of heme as an iron (fe) source by bordetella avium requires bhur, an fe-regulated gene which encodes an outer membrane heme receptor. upstream of bhur is a 507-bp open reading frame, hereby designated rhui (for regulator of heme uptake), which codes for a 19-kda polypeptide. whereas the 19-kda polypeptide had homology to a subfamily of alternative sigma factors known as the extracytoplasmic function (ecf) sigma factors, it was hypothesized that rhui encoded a potential in-t ... | 2001 | 11598070 |
| reciprocal protective immunity against bordetella pertussis and bordetella parapertussis in a murine model of respiratory infection. | the protective immunity induced by infection with bordetella pertussis and with bordetella parapertussis was examined in a murine model of respiratory infection. convalescent mice that had been infected by aerosol with b. pertussis or with b. parapertussis exhibited a protective immune response against b. pertussis and also against b. parapertussis. anti-filamentous hemagglutinin (anti-fha) serum immunoglobulin g (igg) and anti-fha lung iga antibodies were detected in both mice infected with b. ... | 2001 | 11598073 |
| reprogramming of the macrophage transcriptome in response to interferon-gamma and mycobacterium tuberculosis: signaling roles of nitric oxide synthase-2 and phagocyte oxidase. | macrophage activation determines the outcome of infection by mycobacterium tuberculosis (mtb). interferon-gamma (ifn-gamma) activates macrophages by driving janus tyrosine kinase (jak)/signal transducer and activator of transcription-dependent induction of transcription and pkr-dependent suppression of translation. microarray-based experiments reported here enlarge this picture. exposure to ifn-gamma and/or mtb led to altered expression of 25% of the monitored genome in macrophages. the number o ... | 2001 | 11602641 |
| temporal nucleotide changes in pertactin and pertussis toxin genes in bordetella pertussis strains isolated from clinical cases in poland. | growing number of bordetella pertussis infections in 1997-1998 in poland overshadowed the successful national vaccination program. this situation prompted us to investigate if this shift reflects changes in the b. pertussis population. we investigated the possible divergence in genes encoding pertussis toxin subunit 1 (ptxs1) and pertactin (p.69) in b. pertussis population strains during the period of 1960-2000. the pertussis toxin and pertactin variants (ptxs1b and prn1) were found in strains u ... | 2001 | 11672891 |
| molecular characterization and regulation of the aguba operon, responsible for agmatine utilization in pseudomonas aeruginosa pao1. | pseudomonas aeruginosa pao1 utilizes agmatine as the sole carbon and nitrogen source via two reactions catalyzed successively by agmatine deiminase (encoded by agua; also called agmatine iminohydrolase) and n-carbamoylputrescine amidohydrolase (encoded by agub). the aguba and adjacent agur genes were cloned and characterized. the predicted agub protein (m(r) 32,759; 292 amino acids) displayed sequence similarity (< or =60% identity) to enzymes of the beta-alanine synthase/nitrilase family. while ... | 2001 | 11673419 |
| [chronic cough in children: signs of serious disease and investigations]. | only in some particular cases chronic cough requires special investigations. respiratory diseases linked to environment are frequent in children. cough is the most common symptom in child asthma and usually occurs during sleep or exercise. environmental tobacco smoke exposure may concern up to 30% of families. questioning should systematically check for parental smoking in children with chronic cough since avoidance is the only effective treatment. the incidence of whooping cough appears to be i ... | 2001 | 11683088 |
| gene expression in epithelial cells in response to pneumovirus infection. | respiratory syncytial virus (rsv) and pneumonia virus of mice (pvm) are viruses of the family paramyxoviridae, subfamily pneumovirus, which cause clinically important respiratory infections in humans and rodents, respectively. the respiratory epithelial target cells respond to viral infection with specific alterations in gene expression, including production of chemoattractant cytokines, adhesion molecules, elements that are related to the apoptosis response, and others that remain incompletely ... | 2001 | 11686888 |
| ecfe, a new essential inner membrane protease: its role in the regulation of heat shock response in escherichia coli. | we have identified a new protease in escherichia coli, which is required for its viability under normal growth conditions. this protease is anchored in the inner membrane and the gene encoding it has been named ecfe, since it is transcribed by esigma(e) polymerase. multicopy expression of the ecfe gene was found to turn down expression of both esigma(e)- and esigma(32)-transcribed promoters. purified ecfe degrades both heat shock sigma factors rpoe and rpoh in vitro. ecfe has a zinc binding doma ... | 2001 | 11689431 |
| sustained efficacy during the first 6 years of life of 3-component acellular pertussis vaccines administered in infancy: the italian experience. | in 1992-1993, a randomized, double-blind, placebo-controlled clinical trial of two 3-component acellular pertussis vaccines was started in 4 of italy's 20 regions. during the trial, the children had been randomized to receive 3 doses of 1 of 2 acellular pertussis vaccines combined with diphtheria and tetanus toxoids (dt) or of a dt vaccine only, at 2, 4, and 6 months of age. both diphtheria-tetanus-acellular pertussis (dtap) vaccines, 1 manufactured by smithkline beecham (dtap sb; infanrix) and ... | 2001 | 11694665 |
| internalization of bordetella pertussis adenylate cyclase-haemolysin into endocytic vesicles contributes to macrophage cytotoxicity. | bordetella pertussis adenylate cyclase-haemolysin is a critical virulence factor in the murine model of intranasal infection, where it is required for several pathological effects, including macrophage apoptosis. based on biochemical and immunological properties, it was proposed that the toxin was delivered directly to the cytoplasm of eukaryotic cells without trafficking through the endocytic pathway. in the present study, we analysed the cellular distribution of the adenylate cyclase-haemolysi ... | 2001 | 11696032 |
| fasciola hepatica cathepsin l cysteine proteinase suppresses bordetella pertussis-specific interferon-gamma production in vivo. | we have previously demonstrated that fasciola hepatica infection significantly reduced bordetella pertussis-specific interferon (ifn)-gamma production in mice coinfected with b. pertussis or immunized with a pertussis whole cell vaccine (pw). in the present study, we have identified parasite molecules capable of mimicking this suppressive effect of f. hepatica. parenteral injection of mice with culture medium in which adult f. hepatica were maintained (excretory/secretory, es, products) suppress ... | 2001 | 11696165 |
| structural consequences of divalent metal binding by the adenylyl cyclase toxin of bordetella pertussis. | adenylyl cyclase toxin of bordetella pertussis has been shown by several investigators to require ca(2+) for its actions on target cells, but little is known about the nature and specificity of divalent metal binding to this novel toxin. calcium is the preferred divalent metal since toxic actions are markedly reduced in the presence of divalent species other than calcium. mn(2+) epr was used to quantitate and characterize divalent metal binding and revealed that the toxin contains approximately ... | 2001 | 11697853 |
| role of the polymorphic region 1 of the bordetella pertussis protein pertactin in immunity. | in several countries pertussis is re-emerging, despite a high vaccination coverage. it is suggested that antigenic divergence between bordetella pertussis vaccine strains and circulating strains, in particular with respect to pertactin, has contributed to pertussis re-emergence. polymorphism in pertactin is essentially limited to region 1, which is composed of repeats and is located adjacent to an arg-gly-asp motif implicated in adherence. evidence is provided for the immunological relevance of ... | 2001 | 11700340 |
| actinobacillus actinomycetemcomitans harbours type iv secretion system genes on a plasmid and in the chromosome. | nine contiguous genes encoding a potential type iv secretion system have been identified in the chromosome of actinobacillus actinomycetemcomitans strain vt747 and on a plasmid (pvt745) in strain vt745. seven of these genes encode predicted proteins that share significant homology with type iv secretion proteins in bordetella pertussis (ptl operon), brucella melitensis biovar suis and agrobacterium tumefaciens (virb operons), where they are involved in protein secretion, pathogen intracellular s ... | 2001 | 11700353 |
| hcf164 encodes a thioredoxin-like protein involved in the biogenesis of the cytochrome b(6)f complex in arabidopsis. | to understand the biogenesis of the plastid cytochrome b(6)f complex and to identify the underlying auxiliary factors, we have characterized the nuclear mutant hcf164 of arabidopsis and isolated the affected gene. the mutant shows a high chlorophyll fluorescence phenotype and is severely deficient in the accumulation of the cytochrome b(6)f complex subunits. in vivo protein labeling experiments indicated that the mutation acts post-translationally by interfering with the assembly of the complex. ... | 2001 | 11701887 |
| beta-helix model for the filamentous haemagglutinin adhesin of bordetella pertussis and related bacterial secretory proteins. | bordetella pertussis establishes infection by attaching to epithelial cells of the respiratory tract. one of its adhesins is filamentous haemagglutinin (fha), a 500-a-long secreted protein that is rich in beta-structure and contains two regions, r1 and r2, of tandem 19-residue repeats. two models have been proposed in which the central shaft is (i) a hairpin made up of a pairing of two long antiparallel beta-sheets; or (ii) a beta-helix in which the polypeptide chain is coiled to form three long ... | 2001 | 11703654 |
| interchangeability of vaccines. | the delivery of childhood immunizations can be complex, often requiring numerous injections over months or years to complete the recommended schedule. the ability to interchange similar vaccines from different manufacturers during a vaccination series is important because the product administered previously may be unknown or no longer available. | 2001 | 11704720 |
| enzyme degradation and proinflammatory activity in arthritogenic and nonarthritogenic eubacterium aerofaciens cell walls. | two almost-identical strains of eubacterium aerofaciens isolated from the normal human gut flora were used. the cell wall (cw) of one strain with a peptidoglycan (pg) type a4alpha induces chronic arthritis in the rat after a single intraperitoneal injection, whereas cw of the other with pg type a4beta induces only a transient acute arthritis. the cw of the arthritogenic e. aerofaciens was a twofold-more-potent stimulator of the proinflammatory cytokines tumor necrosis factor alpha (tnf-alpha) an ... | 2001 | 11705898 |
| evidence that mycobacterial pe_pgrs proteins are cell surface constituents that influence interactions with other cells. | the elucidation of the genomic sequence of mycobacterium tuberculosis revealed the presence of a novel multigene family designated pe/pe_pgrs that encodes numerous, highly related proteins of unknown function. in this study, we demonstrate that a transposon insertion in a pe_pgrs gene (1818(pe_pgrs)) found in mycobacterium bovis bcg pasteur, which is the bcg homologue of the m. tuberculosis h37rv gene rv1818c, introduces new phenotypic properties to this bcg strain. these properties include disp ... | 2001 | 11705904 |
| regulated antigen expression in live recombinant salmonella enterica serovar typhimurium strongly affects colonization capabilities and specific cd4(+)-t-cell responses. | regulated antigen expression can influence the immunogenicity of live recombinant salmonella vaccines, but a rational optimization has remained difficult since important aspects of this effect are incompletely understood. here, attenuated salmonella enterica serovar typhimurium sl3261 strains expressing the model antigen gfp_ova were used to quantify in vivo antigen levels by flow cytometry and to simultaneously follow the crucial early steps of antigen-specific t-cell responses in mice that are ... | 2001 | 11705925 |
| identification, characterization, and variation in expression of two serologically distinct o-antigen epitopes in lipopolysaccharides of campylobacter fetus serotype a strains. | monoclonal antibodies (mabs) to the lipopolysaccharide (lps) o-antigens of campylobacter fetus serotype a and b strains were produced. eight mabs specific for serotype a lps were characterized on immunoblots of c. fetus serotype a lps. two immunoblot patterns were observed and were used to divide the eight mabs into two groups. mabs m1177 and m1194 were selected as representative of the two groups and were used in an enzyme-linked immunosorbent assay (elisa) to examine the lps o-antigen epitopes ... | 2001 | 11705938 |
| pertussis toxin and lipopolysaccharide influence phagocytosis of bordetella pertussis by human monocytes. | the potential of human monocytes to mediate the clearance of bordetella pertussis infection was examined. bacteria expressing green fluorescent protein were incubated with adherent peripheral blood monocytes, and phagocytosis was quantified by using fluorescence microscopy. monocytes internalized only a small percentage of the adherent bacteria. surface-associated bvg-regulated virulence factors, including adenylate cyclase toxin and filamentous hemagglutinin, did not affect attachment or phagoc ... | 2001 | 11705943 |
| immune response genes modulate serologic responses to vibrio cholerae tcpa pilin peptides. | cholera is an enteric disease caused by vibrio cholerae. toxin-coregulated pilus (tcp), a type 4 pilus expressed by v. cholerae, is a cholera virulence factor that is required for host colonization. the tcp polymer is composed of subunits of tcpa pilin. antibodies directed against tcpa are protective in animal models of cholera. while natural or recombinant forms of tcpa are difficult to purify to homogeneity, it is anticipated that synthesized tcpa peptides might serve as immunogens in a subuni ... | 2001 | 11705949 |
| antimicrobial susceptibility testing of clinical isolates of bordetella pertussis from northern california: report from the sentry antimicrobial surveillance program. | reports of an increased clinical incidence of pertussis and the development of resistance by bordetella pertussis to erythromycin prompted the collection and testing of recent clinical isolates from patients in northern california against a range of antimicrobial agents by the etest (ab biodisk, solna, sweden) method. all isolates were fully susceptible to all eight agents tested (mic, <or=0.38 microg/ml), including newer fluoroquinolones, such as gatifloxacin (mic of which 90% of the isolates t ... | 2001 | 11709347 |
| fimbrial typing of bordetella pertussis isolates: agglutination with polyclonal and monoclonal antibodies. | 2001 | 11712516 | |
| fc receptor-mediated immunity against bordetella pertussis. | the relevance of specific abs for the induction of cellular effector functions against bordetella pertussis was studied. igg-opsonized b. pertussis was efficiently phagocytosed by human polymorphonuclear leukocytes (pmn). this process was mediated by the pmn igg receptors, fcgammariia (cd32) and fcgammariiib (cd16), working synergistically. furthermore, these fcgammar triggered efficient pmn respiratory burst activity and mediated transfer of b. pertussis to lysosomal compartments, ultimately re ... | 2001 | 11714823 |
| redox signal transduction by the arcb sensor kinase of haemophilus influenzae lacking the pas domain. | the arc (anoxic redox control) two-component signal transduction system of escherichia coli, which comprises the tripartite arcb sensor kinase and the arca response regulator, modulates the expression of numerous operons in response to redox conditions of growth. we demonstrate that the arca and arcb genes of haemophilus influenzae specify a two-component system. the arc proteins of the two bacterial species sufficiently resemble each other that they can participate in heterologous transphosphor ... | 2001 | 11717280 |
| polymorphism of bordetella pertussis isolates circulating for the last 10 years in france, where a single effective whole-cell vaccine has been used for more than 30 years. | we compared bordetella pertussis isolates collected in france over the last 10 years, the vaccine strains used for more than 30 years, and isolates collected before the introduction of generalized vaccination. the analysis included serotyping, pulsed-field gel electrophoresis of chromosomal dna after digestion with xbai and spei, and sequencing of the pt s1 gene, encoding the s1 subunit of pertussis toxin, and the prn gene, encoding the adhesin pertactin. we found that the incidence of infection ... | 2001 | 11724851 |
| construction of mini-tn5cyaa' and its utilization for the identification of genes encoding surface-exposed and secreted proteins in bordetella bronchiseptica. | a mini-tn5 transposon derivative, mini-tn5cyaa', has been constructed. it contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of bordetella pertussis adenylate cyclase toxin (cyaa'). we used this system to mutagenize b. bronchiseptica and we developed a screen for identification of mutants containing cyaa' translational fusions. this system was used to identify b. bronchiseptica genes that encode surface-exposed and secreted proteins. | 2001 | 11728725 |
| immunologic and epidemiologic experience of vaccination with a monocomponent pertussis toxoid vaccine. | pertussis re-emerged in sweden with a cumulative incidence of about 60% during the first 10 years of life, when the locally produced cellular vaccine lost its efficacy around 1970 and general vaccination was discontinued in 1979. the epidemiology, clinical features, and immunology of pertussis and a monocomponent pertussis toxoid vaccine were studied in göteborg, sweden. after phase 1 and 2 studies, a randomized, double-blind, placebo-controlled trial of pertussis toxoid (ptox), compounded with ... | 2001 | 11731642 |
| proliferating macrophages, dendritic cells, natural killer cells, t and b lymphocytes in the middle ear and eustachian tube mucosa during experimental acute otitis media in the rat. | although many studies focus on the increase of immunocompetent cells within the middle ear mucosa during acute otitis media it is poorly understood how this increase is mediated. the differentiation between two possible causes, i.e. immigration and local proliferation, would help to better understand the pathophysiology of this disease. therefore, the number of proliferating macrophages, dendritic cells, natural killer cells and t and b lymphocytes was studied during acute otitis media in the ra ... | 2001 | 11737056 |
| tetanus toxin fragment c-specific priming by intranasal infection with recombinant bordetella pertussis. | as an alternative to parenteral administration, mucosal administration offers several advantages including the ease of administration, safety and the ability to induce mucosal immunity. as a first step towards nasal administration of important childhood vaccines, we have previously developed attenuated bordetella pertussis strains able to protect mice against pertussis upon nasal vaccination. since pertussis vaccines are generally combined with tetanus and diphtheria vaccines, we constructed rec ... | 2001 | 11738759 |
| efficacy and safety of clarithromycin versus erythromycin for the treatment of pertussis: a prospective, randomized, single blind trial. | pertussis is still a prevalent public health problem, and antibiotic therapy may decrease disease severity and limit communicability. erythromycin is the recommended antibiotic for treatment and prophylaxis of pertussis; however, side effects of erythromycin limit its usefulness in some patients. clarithromycin, a newer macrolide, has good in vitro activity against bordetella pertussis and a better side effect profile. | 2001 | 11740322 |
| inhibitory effect on immunoglobulin e production in vivo and in vitro by siegesbeckia glabrescens. | elevated levels of immunoglobulin (ig)e are associated with immediate-type allergic reactions. the effect of an aqueous extract of siegesbeckia glabrescens (compositae) whole plants (sgwp) on in vivo and in vitro ige production was studied in mice. sgwp dose-dependently inhibited the active systemic anaphylaxis and serum ige production induced by immunization with ovalbumin and bordetella pertussis toxin absorbed to aluminium hydroxide gel. sgwp dose-dependently inhibited il-4-dependent ige prod ... | 2001 | 11746835 |
| rapid identification of bordetella pertussis pertactin gene variants using lightcycler real-time polymerase chain reaction combined with melting curve analysis and gel electrophoresis. | recently, eight allelic variants of the pertactin gene (prn1-8) have been characterized in bordetella pertussis strains isolated in europe and the united states. it has been suggested that the divergence of the pertactin types of clinical isolates from those of the b. pertussis vaccine strains is a result of vaccine-driven evolution. sequencing of the prn, which is relatively time-consuming, has so far been the only method for the differentiation of prn types. we have developed a rapid real-time ... | 2001 | 11747721 |
| growth phase influences complement resistance of bordetella pertussis. | the brka protein of bordetella pertussis inhibits killing by the antibody-dependent classical pathway of complement; however, susceptibility to complement can be highly variable. log-phase bacteria grown in stainer-scholte (ss) broth plated on bordet-gengou (bg) agar were about 500 times more sensitive to killing by complement than stationary-phase ss-bg cultures. while always more susceptible to complement than the wild-type strain, a brka mutant displayed a similar growth phase variation in su ... | 2002 | 11748208 |
| induction of the cytokine signal regulator socs3/cis3 as a therapeutic strategy for treating inflammatory arthritis. | immune and inflammatory systems are controlled by multiple cytokines, including ils and infs. these cytokines exert their biological functions through janus tyrosine kinases and stat transcription factors. one such cytokine, il-6, has been proposed to contribute to the development of rheumatoid arthritis (ra). we found that stat3 was strongly tyrosine phosphorylated in synovial tissue of ra patients, but not those with osteoarthritis. blockade of the il-6-gp130-jak-stat3-signaling pathway might ... | 2001 | 11748261 |
| changes in predominance and diversity of genomic subtypes of bordetella pertussis isolated in the united states, 1935 to 1999. | pulsed-field gel electrophoresis (pfge) of bordetella pertussis chromosomal dna fragments generated by xbai restriction has been used to subtype isolates for epidemiologic studies. to better understand the natural history of pertussis, we determined the pfge profiles of 1,333 strains isolated in the united states from 1935 to 1999. results showed a shift in prevalent profiles from the earliest to the latest study periods. in addition, genetic diversity decreased over time, and prevalent profiles ... | 2002 | 11749747 |
| differences in signal transduction of two 5-ht4 receptor splice variants: compound specificity and dual coupling with galphas- and galphai/o-proteins. | this study documents differences in ligand binding and signal transduction properties between the human (h) 5-hydroxytryptamine (5-ht)4a and h5-ht4b receptor splice variants stably expressed in human embryonic kidney 293 cells. the fraction of the [3h]5-ht high-affinity site relative to the whole receptor population measured with [3h]gr113808 was higher for the h5-ht4a isoform (around 0.4) than for the 5-ht4b isoform (around 0.2) and was independent of the level of expression. the potency and ef ... | 2002 | 11752209 |
| betawrap: successful prediction of parallel beta -helices from primary sequence reveals an association with many microbial pathogens. | the amino acid sequence rules that specify beta-sheet structure in proteins remain obscure. a subclass of beta-sheet proteins, parallel beta-helices, represent a processive folding of the chain into an elongated topologically simpler fold than globular beta-sheets. in this paper, we present a computational approach that predicts the right-handed parallel beta-helix supersecondary structural motif in primary amino acid sequences by using beta-strand interactions learned from non-beta-helix struct ... | 2001 | 11752429 |
| differential clearance and induction of host responses by various administered or released lipopolysaccharides. | the clearance and activity of different types of lipopolysaccharide (lps) released during infection with gram-negative bacteria were investigated. when highly purified preparations differing in their specific endotoxin activity were administered intravenously to mice, the clearance of rough (r)-form lps preparations from salmonella minnesota and escherichia coli was much faster than that of a smooth (s)-form lps preparation from salmonella abortus equi, but slower than that of lipo-oligosacchari ... | 2001 | 11753211 |
| [susceptibility to macrolide antibiotics of bordetella pertussis and bordetella parapertussis strains isolated from whooping cough patients in 1968 and in 1997-99]. | the activity of erythromycin, azithromycin, clarithromycin, dirithromycin, oleandomycin, roxithromycin, spiramycin and josamycin against 21 and 34 b. pertussis strains and against 6 and 8 b. parapertussis strains isolated respectively in the years 1968 and 1997-99 was examined. the antibiotic agar dilution method was used. the minimum concentration of macrolides which inhibited growth of b. pertussis and b. parapertussis was calculated for 50% (mic50) and 90% (mic90) of isolates. the susceptibil ... | 2001 | 11757403 |
| dual-substrate utilization by bordetella pertussis. | to improve the cultivation of bordetella pertussis and take advantage of the newest techniques in monitoring and control, a quantitative description of substrate utilisation is necessary. growth of the organism is limited by two main substrates. however neither interactive nor non-interactive modelling seem appropriate. a model that combines essential and enhanced kinetics was developed based on experimental observation. instead of fitting all model parameters at once, a step-wise experimentatio ... | 2001 | 11762593 |
| bordetella pertussis: increasing problems with a well-known pathogen and its relatives. | 2001 | 11764730 | |
| [a pertussis outbreak in a ward for severely retarded]. | in june 2000, many cases with persistent cough were observed among inpatients and the staff of a ward for severely retarded. some of them had symptoms suggestive of pertussis, such as whooping, post-tussive apnea. we performed a retrospective investigation to assess symptoms and serological findings suspicious of pertussis. there were a total of 14 cases of persistent cough over 3 weeks (4 to 9 weeks). 6 cases were inpatients and 8 were hospital staff. of those, serological test for pertussis in ... | 2001 | 11766374 |
| characterization of recombinant bordetella pertussis adenylate cyclase toxins carrying passenger proteins. | bordetella pertussis secretes a calmodulin-activated adenylate cyclase toxin, cyaa, that is able to deliver its n-terminal catalytic domain (400 amino acid residues) into the cytosol of eukaryotic target cells, directly through the cytoplasmic membrane. we have previously shown that cyaa can be used as a vehicle to deliver cd8+ t-cell epitopes, inserted within the catalytic domain of the toxin, into antigen-presenting cells and can trigger specific class i-restricted cytotoxic t-cell (ctl) respo ... | 2001 | 11766964 |
| multiplex lightcycler pcr assay for detection and differentiation of bordetella pertussis and bordetella parapertussis in nasopharyngeal specimens. | a rapid real-time multiplex pcr assay for detecting and differentiating bordetella pertussis and bordetella parapertussis in nasopharyngeal swabs was developed. this assay (lc-pcr-is) targets the insertion sequences is481 and is1001 of b. pertussis and b. parapertussis, respectively, and is performed using the lightcycler (roche molecular biochemicals, indianapolis, ind.). the analytical sensitivity is less than one organism per reaction. results for bordetella culture and/or direct fluorescent ... | 2002 | 11773099 |
| investigation of role of nitric oxide in protection from bordetella pertussis respiratory challenge. | the mechanism whereby whole-cell pertussis vaccines (wcv) confer protection against bordetella pertussis is still not fully understood. we have previously reported that macrophage activation produced by vaccination with wcv is associated with induction of no synthesis by macrophages in response to in vitro stimulation with b. pertussis antigens. to determine whether no production is an effector of protection or simply a marker of activation, the susceptibility of inducible nitric oxide synthase ... | 2002 | 11796599 |
| delivery of a male cd4(+)-t-cell epitope into the major histocompatibility complex class ii antigen presentation pathway by bordetella pertussis adenylate cyclase. | recombinant adenylate cyclase toxoids are shown to deliver inserted foreign cd4(+)-t-cell epitopes into the major histocompatibility complex class ii presentation pathway, inducing a specific cd4(+)-t-cell response in vivo and yielding in vitro stimulation of specific cd4(+) t cells at a 100-times-higher molar efficiency than the free peptide containing the epitope. | 2002 | 11796640 |
| pathogen-specific t regulatory 1 cells induced in the respiratory tract by a bacterial molecule that stimulates interleukin 10 production by dendritic cells: a novel strategy for evasion of protective t helper type 1 responses by bordetella pertussis. | antigen-specific t helper type 1 (th1) cells mediate protective immunity against a range of infectious diseases, including that caused by bordetella pertussis. distinct t cell subtypes that secrete interleukin (il)-10 or tumor growth factor (tgf)-beta are considered to play a role in the maintenance of self-tolerance. however, the antigens recognized by these regulatory t cells in vivo have not been defined. here we provide the first demonstration of pathogen-specific t regulatory type 1 (tr1) c ... | 2002 | 11805149 |
| significance of two distinct types of tryptophan synthase beta chain in bacteria, archaea and higher plants. | tryptophan synthase consists of two subunits, alpha and beta. two distinct subgroups of beta chain exist. the major group (trpeb_1) includes the well-studied beta chain of salmonella typhimurium. the minor group of beta chain (trpeb_2) is most frequently found in the archaea. most of the amino-acid residues important for catalysis are highly conserved between both trpe subfamilies. | 2002 | 11806827 |
| comparative analysis of fur regulons in gamma-proteobacteria. | iron is an essential element for the survival and pathogenesis of bacteria. the strict control of iron homeostasis is mediated by the fur repressor, which is highly conserved among various bacterial species. here we apply the comparative genomics approach to analyze candidate fur-binding sites in the genomes of escherichia coli (k12 and o157:h7), salmonella typhi, yersinia pestis and vibrio cholerae. we describe a number of new loci encoding siderophore biosynthesis and transport proteins. a new ... | 2001 | 11812853 |
| efficacy of pertussis components in an acellular vaccine, as assessed in a murine model of respiratory infection and a murine intracerebral challenge model. | the efficacy of 10 pertussis vaccines prepared from various concentrations of pertussis toxin (pt) and filamentous hemagglutinin (fha) was investigated in a murine model of respiratory infection (aerosol challenge model) and a murine intracerebral (ic) challenge model. pt was necessary as a vaccine component for protection against an ic challenge with bordetella pertussis. fha appeared to play an important role as a vaccine component in protection against an aerosol challenge with b. pertussis. ... | 2002 | 11818163 |
| [comparison of three elisa techniques for the evaluation of igg seroprevalence against bordetella pertussis in children vaccinated with three doses of dtpwc]. | although there are several elisa procedures to determine igg against bordetella pertussis, there are scarce data that allow to compare the seroprevalence detected using different kits. the objective of this study was to evaluate and to compare by three elisa techniques the seroprevalence against b. pertussis in children. | 2002 | 11820974 |
| mitochondrial alterations precede bordetella pertussis-induced apoptosis. | bordetella pertussis, the causative agent of whooping cough in humans, secretes a number of toxins, including adenylate cyclase-hemolysin (ac-hly), and induces macrophage apoptosis. we investigated the effects of b. pertussis on mitochondrial membrane potential (deltapsim) and atp levels, as possible determinants of cell death. using the fluorescent probe jc-1, we found that infection of human monocytes by b. pertussis lead to a disruption in host-cell deltapsim. deltapsim alterations were prece ... | 2002 | 11821234 |
| microbial compounds selectively induce th1 cell-promoting or th2 cell-promoting dendritic cells in vitro with diverse th cell-polarizing signals. | upon microbial infection, specific th1 or th2 responses develop depending on the type of microbe. here, we demonstrate that different microbial compounds polarize the maturation of human myeloid dendritic cells (dcs) into stably committed th1 cell-promoting (dc1) or th2 cell-promoting (dc2) effector dcs that polarize th cells via different mechanisms. protein extract derived from the helminth schistosoma mansoni induced the development of dc2s that promote the development of th2 cells via the en ... | 2002 | 11823500 |
| characteristics and potency of an acellular pertussis vaccine composed of pertussis toxin, filamentous hemagglutinin, and pertactin. | in an attempt to develop a safer pertussis vaccine, we successfully purified 3 pertussis protective antigens-pertussis toxin, filamentous hemagglutinin, and a 69-kda outer membrane protein (also named pertactin), from bordetella pertussis strain atcc 9340. the toxicity of pertussis toxin could be effectively reduced by the treatment with formaldehyde 0.07% while preserving of a high degree of immunogenicity. by mixing purified pertussis antigens with diphtheria and tetanus toxoids (dt), we have ... | 2001 | 11825003 |
| the use of taqman pcr assay for detection of bordetella pertussis infection from clinical specimens. | the routine clinical laboratory detection of bordetella pertussis is through culture, which can require 5 to 7 days for the bacteria to grow. using a polymerase chain reaction (pcr) assay can shorten this detection time while increasing the sensitivity of detection with similar specificity. this study compared culture with taqman pcr for detection of b pertussis in clinical specimens and the turnaround time for each assay during the pertussis season. | 2002 | 11825113 |
| the brucella suis virb operon is induced intracellularly in macrophages. | a type iv secretion system similar to the virb system of the phytopathogen agrobacterium tumefaciens is essential for the intracellular survival and multiplication of the mammalian pathogen brucella. reverse transcriptase-pcr showed that the 12 genes encoding the brucella suis virb system form an operon. semiquantitative measurements of virb mrna levels by slot blotting showed that transcription of the virb operon, but not the flanking genes, is regulated by environmental factors in vitro. flow ... | 2002 | 11830669 |
| stability of antibodies to bordetella antigens in german adults. | to estimate the rate of asymptomatic exposure to bordetella pertussis antigens in the german adult population and to evaluate the stability of antibodies to these antigens, antibody levels against bordetella antigens and their variability over time were measured in german adult blood donors. one hundred forty-six regular blood donors (41 females, 105 males) were tested repeatedly for antibodies of isotypes igg and iga to pertussis toxin, filamentous hemagglutinin (fha) and pertactin over a perio ... | 2001 | 11837635 |
| not every truth is good. the dangers of publishing knowledge about potential bioweapons. | 2002 | 11839688 | |
| yopd and lcrh regulate expression of yersinia enterocolitica yopq by a posttranscriptional mechanism and bind to yopq rna. | pathogenic yersiniae secrete 14 yop proteins via the type iii pathway. synthesis of yopq occurs when the type iii machinery is activated by a low-calcium signal, but not when the calcium concentration is above 100 microm. to characterize the mechanism that regulates the expression of yopq, mutants that permit synthesis of yopq in the presence of calcium were isolated. yersiniae bearing deletion mutations in yopn, tyea, sycn, or yscb synthesized and secreted yopq in both the presence and the abse ... | 2002 | 11844757 |
| mutations in the thiol-disulfide oxidoreductases bdbc and bdbd can suppress cytochrome c deficiency of ccda-defective bacillus subtilis cells. | cytochromes of the c type in the gram-positive bacterium bacillus subtilis are all membrane anchored, with their heme domains exposed on the outer side of the cytoplasmic membrane. they are distinguished from other cytochromes by having heme covalently attached by two thioether bonds. the cysteinyls in the heme-binding site (cxxch) in apocytochrome c must be reduced in order for the covalent attachment of the heme to occur. it has been proposed that ccda, a membrane protein, transfers reducing e ... | 2002 | 11844773 |
| membrane localization of the s1 subunit of pertussis toxin in bordetella pertussis and implications for pertussis toxin secretion. | pertussis toxin is secreted from bordetella pertussis with the assistance of the ptl transport system, a member of the type iv family of macromolecular transporters. the s1 subunit and the b oligomer combine to form the holotoxin prior to export from the bacterial cell, although the site of assembly is not known. to better understand the pathway of pertussis toxin assembly and secretion, we examined the subcellular location of the s1 subunit, expressed with or without the b oligomer and the ptl ... | 2002 | 11854200 |
| the dot/icm type iv secretion system of legionella pneumophila is essential for the induction of apoptosis in human macrophages. | we have previously shown that legionella pneumophila induces caspase 3-dependent apoptosis in mammalian cells during early stages of infection. in this report, we show that nine l. pneumophila strains with mutations in the dota, dotdcb, icmt, icmgcd, and icmjb loci are completely defective in the induction of apoptosis, in addition to their severe defects in intracellular replication and pore formation-mediated cytotoxicity. importantly, all nine dot/icm mutants were complemented for all their d ... | 2002 | 11854262 |
| role of immune sera in the in-vitro phagocytosis of bordetella pertussis strains. | in this study, phagocytosis of bordetella pertussis was assessed using a human monocyte-derived macrophage line (thp-1) and immune sera from children who had received primary vaccination during the italian clinical trial on the efficacy of two acellular three-component (pt-fha-prn) and one whole-cell pertussis vaccines. the results demonstrate that phagocytosis of opsonized bacteria with specific immune sera is not significantly enhanced compared with that of non-opsonized bacteria or bacteria o ... | 2002 | 11855944 |
| polymerase chain reaction (pcr) compared with conventional identification in culture for detection of bordetella pertussis in 7153 children. | objective: to compare the polymerase chain reaction (pcr) results with conventional culture results for the diagnosis of bordetella pertussis infections. methods: pcr and culture were performed in the course of a large vaccine efficacy trial in germany on specimens taken from 7153 children less-than-or-equal2 years of age with cough illness lasting >6 days, and laboratory results were compared with clinical data also obtained from the patients. calcium alginate nasopharyngeal swabs were taken fo ... | 1997 | 11864157 |
| differential modulation of ca(v)2.1 channels by calmodulin and ca2+-binding protein 1. | ca(v)2.1 channels, which mediate p/q-type ca2+ currents, undergo ca2+/calmodulin (cam)-dependent inactivation and facilitation that can significantly alter synaptic efficacy. here we report that the neuronal ca2+-binding protein 1 (cabp1) modulates ca(v)2.1 channels in a manner that is markedly different from modulation by cam. cabp1 enhances inactivation, causes a depolarizing shift in the voltage dependence of activation, and does not support ca2+-dependent facilitation of ca(v)2.1 channels. t ... | 2002 | 11865310 |
| enterobacterial intergenic consensus sequence polymerase chain reaction as a typing method for burkholderia (pseudomonas) cepacia. | objective: we developed a rapid polymerase chain reaction (pcr) method, which utilizes primers from an enterobacterial repetitive consensus sequence, to differentiate among strains of burkholderia cepacia. we then applied this method to isolates from a device-associated pseudoinfection and also compared the discriminative typing potential with that of another pcr-based method. methods: a simplified lysis procedure was used to generate dna substrate for amplification by a pcr method which utilize ... | 1996 | 11866813 |
| bordetella interspecies allelic variation in alcr inducer requirements: identification of a critical determinant of alcr inducer responsiveness and construction of an alcr(con) mutant allele. | previous studies established the critical roles of alcr and alcaligin inducer in positive regulation of alcaligin siderophore biosynthesis and transport genes in bordetella pertussis and bordetella bronchiseptica. transcriptional analyses using plasmid-borne alcr genes of b. pertussis ut25 and b. bronchiseptica b013n to complement the alcr defect of b. bronchiseptica strain brm13 (delta alcr1 alca::mini-tn5 lacz1) revealed interspecies differences in alcr inducer requirements for activation of a ... | 2002 | 11872703 |
| reproducibility of bordetella pertussis genomic dna fragments generated by xbai restriction and resolved by pulsed-field gel electrophoresis. | the intra- and interlaboratory variabilities of the molecular size measurements of each dna fragment contributing to three pulsed-field gel electrophoresis (pfge) profiles were assessed, as were the reproducibilities of the entire pfge profiles for three bordetella pertussis strains. the major source of variability within a laboratory occurred between subcultures rather than within gels or between gels. each pfge profile was generated reproducibly and was objectively defined by the molecular siz ... | 2002 | 11880398 |
| novel pcr-probe assay for detection of and discrimination between legionella pneumophila and other legionella species in clinical samples. | 2002 | 11880461 | |
| genomic analyses of bacterial respiratory and cytochrome c assembly systems: bordetella as a model for the system ii cytochrome c biogenesis pathway. | an analysis of thirty-three genomes of selected bacteria for the presence of specific respiratory pathways and cytochrome c biogenesis systems has led to observations on respiration and biogenesis. a table summarizing these results is presented. the data suggested that bordetella pertussis would be an excellent genetic model to study the system ii cytochrome c biogenesis pathway. these observations are discussed and the results of genetic studies on system ii biogenesis in b. pertussis are prese ... | 2002 | 11881892 |