Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities. | fifteen bacterial strains isolated from solid waste oil samples were selected due to their capacity of growing in the presence of hydrocarbons. the isolates were identified by pcr of the 16s rdna gene using fd1 and rd1 primers. the majority of the strains belonged to genera bacillus, bacillus pumilus (eight strains) and bacillus subtilis (two strains). besides, three strains were identified as micrococcus luteus, one as alcaligenes faecalis and one strain as enterobacter sp. growth of the above- ... | 2006 | 16564960 |
| tight binding inhibitors of n-acyl amino sugar and n-acyl amino acid deacetylases. | very potent inhibitors were synthesized for the enzymatic deacetylation of n-acetyl-d-glucosamine-6-phosphate (naga) and n-acetyl-d-glutamate (dgd). the methyl phosphonamidate derivative of d-glucosamine-6-phosphate bound to n-acetyl-d-glucosamine-6-phosphate deacetylase with an equilibrium dissociation constant of 34 +/- 5 nm at ph 7.5 and an association rate constant of 6.1 x 103 m-1 s-1. the inhibition constant is 4000-fold lower than the michaelis constant for the substrate n-acetyl-d-glucos ... | 2006 | 16568996 |
| a rearranging ligand enables allosteric control of catalytic activity in copper-containing nitrite reductase. | in cu-containing nitrite reductase from alcaligenes faecalis s-6 the axial methionine ligand of the type-1 site was replaced (m150g) to make the copper ion accessible to external ligands that might affect the enzyme's catalytic activity. the type-1 site optical spectrum of m150g (a(460)/a(600)=0.71) differs significantly from that of the native nitrite reductase (a(460)/a(600)=1.3). the midpoint potential of the type-1 site of nitrite reductase m150g (e(m)=312(+/-5)mv versus hydrogen) is higher ... | 2006 | 16574144 |
| castellaniella gen. nov., to accommodate the phylogenetic lineage of alcaligenes defragrans, and proposal of castellaniella defragrans gen. nov., comb. nov. and castellaniella denitrificans sp. nov. | comparative 16s rrna gene sequence analysis indicates that two distinct sublineages exist within the genus alcaligenes: the alcaligenes faecalis lineage, comprising alcaligenes aquatilis and a. faecalis (with the three subspecies a. faecalis subsp. faecalis, a. faecalis subsp. parafaecalis and a. faecalis subsp. phenolicus), and the alcaligenes defragrans lineage, comprising a. defragrans. this phylogenetic discrimination is supported by phenotypic and chemotaxonomic differences. it is proposed ... | 2006 | 16585701 |
| effect of overexpression of a soluble pyridine nucleotide transhydrogenase (udha) on the production of poly(3-hydroxybutyrate) in escherichia coli. | a soluble pyridine nucleotide transhydrogenase (udha) has been used to increase the productivity and yield of phb in vivo. by inducing a high level of udha, which can transfer reducing equivalents between nad and nadp, we have increased nadph availability, resulting in high yield and productivity of phb in escherichia coli. coexpression of the phb operon from alcaligenes eutrophus h16 and the native udha from e. coli from high copy plasmids resulted in an increase in phb yield from 49 to 66% g o ... | 2006 | 16599556 |
| antibiotic resistance and identification of uncommon gram-negative bacteria isolated from sputum of adult patients with cystic fibrosis. | 2006 | 16613138 | |
| a random-sequential mechanism for nitrite binding and active site reduction in copper-containing nitrite reductase. | the homotrimeric copper-containing nitrite reductase (nir) contains one type-1 and one type-2 copper center per monomer. electrons enter through the type-1 site and are shuttled to the type-2 site where nitrite is reduced to nitric oxide. to investigate the catalytic mechanism of nir the effects of ph and nitrite on the turnover rate in the presence of three different electron donors at saturating concentrations were measured. the activity of nir was also measured electrochemically by exploiting ... | 2006 | 16613859 |
| atomic description of an enzyme reaction dominated by proton tunneling. | we present an atomic-level description of the reaction chemistry of an enzyme-catalyzed reaction dominated by proton tunneling. by solving structures of reaction intermediates at near-atomic resolution, we have identified the reaction pathway for tryptamine oxidation by aromatic amine dehydrogenase. combining experiment and computer simulation, we show proton transfer occurs predominantly to oxygen o2 of asp(128)beta in a reaction dominated by tunneling over approximately 0.6 angstroms. the role ... | 2006 | 16614214 |
| characterization of bacterial beta-carotene 3,3'-hydroxylases, crtz, and p450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in escherichia coli. | beta-carotene hydroxylase (crtz) is one of rate-limiting enzymes for astaxanthin production. a complementation analysis was conducted using escherichia coli transformants to compare the catalytic efficiency of bacterial crtz from brevundimonas sp. sd212, paracoccus sp. pc1 (formerly known as alcaligenes sp. pc-1), paracoccus sp. n81106 (agrobacterium aurantiacum), pantoea ananatis (erwinia uredovora 20d3), marine bacterium p99-3, and p450 monooxygenase (cyp175a1) from thermus thermophilus hb27. ... | 2006 | 16614859 |
| metabolism of [14c]-2,4-dichlorophenol in edible plants. | several 2,4-dichlorophenoxyacetic acid (2,4-d)-sensitive plants have been modified by genetic engineering with tfda gene to acquire 2,4-d tolerance. the expression product of this gene degrades 2,4-d to 2,4-dichlorophenol (dcp), which is less phytotoxic but could cause a problem of food safety. after a comparison of 2,4-d and dcp metabolism in transgenic 2,4-d-tolerant and wild cotton (gossypium hirsutum l.), a direct study of dcp metabolism in edible plants was performed. after petiolar uptake ... | 2006 | 16628540 |
| isolation and characterization of rhizosphere bacteria with potential for biological control of weeds in vineyards. | deleterious rhizosphere inhabiting bacteria (drb) have potential to suppress plant growth. this project focuses on the isolation of drb with potential for development as commercial products for weed control. | 2006 | 16629995 |
| purification and characterization of a novel caffeine oxidase from alcaligenes species. | alcaligenes species cf8 isolated from surface water of a lake produced a novel serine type metallo-caffeine oxidase. the optimal medium for caffeine oxidase production by this strain was (w/v) nano(3), 0.4%; kh(2)po(4), 0.15%; na(2)hpo(4), 0.05%; fecl(3).6h(2)o, 0.0005%; cacl(2).2h(2)o, 0.001%; mgso(4).7h(2)o, 0.02%; glucose, 0.2%; caffeine, 0.05%, ph 7.5. the enzyme was purified to 63-fold by using ammonium sulfate precipitation, dialysis, ion exchange (diethylaminoethyl-cellulose) and gel filt ... | 2006 | 16647778 |
| activities, occurrence, and localization of hydrogenase in free-living and symbiotic frankia. | symbiotic and free-living frankia were investigated for correlation between hydrogenase activities (in vivo/in vitro assays) and for occurrence and localization of hydrogenase protein by western blots and immuno-gold localization, respectively. freshly prepared nodule homogenates from the symbiosis between alnus incana and a local source of frankia did not show any detectable in vivo or in vitro hydrogenase uptake activity, as also has been shown earlier. however, a free-living frankia strain or ... | 1990 | 16667353 |
| stereoselective nitrile hydrolysis by immobilized whole-cell biocatalyst. | the present work attempts to deal with the stability and reusability aspect of nitrilase from alcaligenes faecalis for the production of (r)-(-)-mandelic acid. four entrapment matrixes were screened to search for a suitable support, and alginate was found to have significant process advantages over its other counterparts. thermodynamic analysis allowed us to account for decreased enantioselectivity (e) as a result of immobilization. the system was also characterized based on the thiele modulus ( ... | 2006 | 16677036 |
| structure solution of azurin ii from alcaligenes xylosoxidans using the laue method: possibility of studying in situ redox changes using x-rays. | we have recently demonstrated that x-rays can be used for changing the redox states of the metal centre in metalloproteins [murphy et al. (1995). j. synchrotron rad. 2, 64-69]. the possibility of using the laue method for studying the structural changes associated with such x-ray-induced reactions is explored by applying the method to the structure determination of a new azurin (hereafter referred to as azurin ii) from the denitrifying bacterium alcaligenes xylosoxidans. laue x-ray diffraction d ... | 1996 | 16702653 |
| characterization of a novel long-chain acyl-coa thioesterase from alcaligenes faecalis. | a novel long-chain acyl-coa thioesterase from alcaligenes faecalis has been isolated and characterized. the protein was extracted from the cells with 1 m nacl, which required 1.5-fold, single-step purification to yield near-homogeneous preparations. in solution, the protein exists as homomeric aggregates, of mean diameter 21.6 nm, consisting of 22-kda subunits. ms/ms data for peptides obtained by trypsin digestion of the thiosterase did not match any peptide from escherichia coli thioesterases o ... | 2006 | 16704412 |
| fatal alcaligenes xylosoxidans infection of the liver: presenting as a liver mass after cholecystectomy. | 2006 | 16725005 | |
| mutant afm 2 of alcaligenes faecalis for phenol biodegradation using he-ne laser irradiation. | he-ne laser technology was utilized in this study to investigate the response of alcaligenes faecalis to laser stimulation. the irradiation experiments were conducted by the adjustment of the output power from 5 to 25 mw and the exposure time from 5 to 25 min. the results showed that the survival rate changed regularly with the variety of irradiation dose, and high positive mutation frequency was determined by both the energy density and the output power. the mutant strain afm 2 was obtained. ph ... | 2006 | 16730779 |
| tetrathiobacter mimigardefordensis sp. nov., isolated from compost, a betaproteobacterium capable of utilizing the organic disulfide 3,3'-dithiodipropionic acid. | in this study, a novel betaproteobacterium, strain dpn7(t), was isolated under mesophilic conditions from compost because of its capacity to utilize the organic disulfide 3,3'-dithiodipropionic acid. analysis of the 16s rrna gene sequence of strain dpn7(t) revealed 98.5 % similarity to that of tetrathiobacter kashmirensis lmg 22695(t). values for sequence similarity to members of the genera alcaligenes, castellaniella and taylorella, the nearest neighbours of the genus tetrathiobacter, were abou ... | 2006 | 16738107 |
| anopheles gambiae (diptera: culicidae) oviposition in response to agarose media and cultured bacterial volatiles. | anopheles gambiae s.s. giles accepted a range of agarose gels, varying from 0.5 to 8% (wt:vol), for oviposition; laid more eggs on 0.5% agarose gels than on moist filter paper or on drier agarose; and laid equal numbers of eggs on 0.5% agarose gels and distilled water. larvae hatched on agarose gel substrates and crawled onto the surface of moist agarose gels, but they tended only to burst the egg cap and remain within the egg case on drier gels. a mixture of cultured bacteria, originating from ... | 2006 | 16739407 |
| studies on bacterial phosphatases: the phosphatases of aerobacter aerogenes, alcaligenes faecalis and bacillus subtilis. | 1938 | 16746654 | |
| [community-acquired pneumonia and bacteraemia due to alcaligenes xylosoxidans]. | 2006 | 16752454 | |
| treatment of paper factory effluent using a phenol degrading alcaligenes sp. under free and immobilized conditions. | treatment of the paper factory effluent was done with free and immobilized cells of a phenol degrading alcaligenes sp. d(2). the free cells could bring a maximum of 99% reduction in phenol and 40% reduction in chemical oxygen demand (cod) after 32 and 20 h of treatment, respectively. in the case of immobilized cells, a maximum of 99% phenol reduction and 70% cod reduction was attained after 20 h of treatment under batch process. in the continuous mode of operation using packed bed reactor, the s ... | 2007 | 16759854 |
| polyhydroxyalkanoate copolymers from forest biomass. | the potential for the use of woody biomass in poly-beta-hydroxyalkanoate (pha) biosynthesis is reviewed. based on previously cited work indicating incorporation of xylose or levulinic acid (la) into phas by several bacterial strains, we have initiated a study for exploring bioconversion of forest resources to technically relevant copolymers. initially, pha was synthesized in shake-flask cultures of burkholderia cepacia grown on 2.2% (w/v) xylose, periodically amended with varying concentrations ... | 2006 | 16761168 |
| a strain of pseudomonas sp. isolated from piggery wastewater treatment systems with heterotrophic nitrification capability in taiwan. | a high concentration of nh(4) (+) in piggery wastewater is major problem in taiwan. therefore, in our study, we isolated native heterotrophic nitrifiers for piggery wastewater treatment. heterotrophic nitrifier as-1 was isolated and characterized from the activated sludge of a piggery wastewater system. sets of triplicate crimp-sealed serum bottles were used to demonstrate the heterotrophic nitrifying capability of strain as-1 in an incubator at 30 degrees c. all serum bottles contained 80 ml me ... | 2006 | 16775792 |
| biofilm formation in an ice cream plant. | the sites of biofilm formation in an ice cream plant were investigated by sampling both the production line and the environment. experiments were carried out twice within a 20-day period. first, stainless steel coupons were fixed to surfaces adjacent to food contact surfaces, the floor drains and the doormat. they were taken for the analysis of biofilm at three different production stages. then, biofilm forming bacteria were enumerated and also presence of listeria monocytogenes was monitored. b ... | 2006 | 16779628 |
| active site structures and the redox properties of blue copper proteins: atomic resolution structure of azurin ii and electronic structure calculations of azurin, plastocyanin and stellacyanin. | understanding how the active site structures of blue copper proteins determine their redox properties is the central structure-function relationship question of this important class of protein, also referred to as cupredoxins. we here describe both experimental and computational studies of azurin, plastocyanin and stellacyanin designed to define more accurately the geometric structures of the active site of the reduced and oxidized species, and thus to understand how these structures determine t ... | 2006 | 16786065 |
| active site comparison of coii blue and green nitrite reductases. | copper-containing nitrite reductases (nirs) possess type 1 (t1) and type 2 (t2) copper sites and can be either green or blue in color owing to differences at their t1 centers. the active sites of a green and a blue nir were studied by utilizing their t1cui/t2coii and t1coii/t2coii-substituted forms. the uv/vis spectra of these derivatives highlight the similarity of the t2 centers in these enzymes and that t1 site differences are also present in the coii forms. the paramagnetic nmr spectra of t1 ... | 2006 | 16795108 |
| immobilization of permeabilized whole cell penicillin g acylase from alcaligenes faecalis using pore matrix crosslinked with glutaraldehyde. | the activity of penicillin g acylase from alcaligenes faecalis increased 7.5-fold when cells were permeabilized with 0.3% (w/v) ctab. the treated cells were entrapped by polyvinyl alcohol crosslinked with boric acid, and crosslinked with 2% (v/v) glutaraldehyde to increase the stability. the conversion yield of penicillin g to 6-aminopenicillanic acid was 75% by immobilized system in batch reaction. no activity was lost after 15 cycles and about 65% enzyme activity was retained at the end of the ... | 2006 | 16799762 |
| eggshell factors influencing eggshell penetration and whole egg contamination by different bacteria, including salmonella enteritidis. | trans-shell infection routes and whole egg contamination of 7 selected bacterial strains; staphylococcus warneri, acinetobacter baumannii, alcaligenes sp., serratia marcescens, carnobacterium sp., pseudomonas sp. and salmonella enteritidis, recovered from egg contents, were studied. the first objective was to correlate bacterial eggshell penetration with various eggshell characteristics and bacterial strains. an agar approach was used to assess the eggshell penetration. the second objective was ... | 2006 | 16822571 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| paralogs of genes encoding metal resistance proteins in cupriavidus metallidurans strain ch34. | cupriavidus (wautersia, ralstonia, alcaligenes) metallidurans strain ch34is a well-studied example of a metal-resistant proteobacterium. genome sequence analysis revealed the presence of a variety of paralogs of proteins that were previously shown to be involved in heavy metal resistance. which advantage has c. metallidurans in maintaining all these paralogs during evolution? paralogs investigated belong to the families rnd (resistance nodulation cell division) or chr (chromate resistance). the ... | 2006 | 16825791 |
| coordinate synthesis of azurin i and copper nitrite reductase in alcaligenes xylosoxidans during denitrification. | the denitrifying bacterium alcaligenes xylosoxidans synthesises two azurins (az), which are termed az i and az 2. both function as effective electron donors to copper nitrite reductase (nir) in vitro. as a first step towards identifying the physiological relevance of these electron transfer proteins in the denitrification process, the gene (azua) encoding az i was characterised and its expression with respect to denitrification determined. we show that the azua gene from a. xylosoxidans is monoc ... | 2006 | 16832626 |
| synthesis of phas from waster under various c:n ratios. | polyhydroxyalkanoates (phas) production was carried out under various c:n ratios. a ratio of 100 resulted best polymer yield. c-source was an important factor in synthesis. for example, as the ratio of valeric acid (c5) to butyric acid (c4) in n-free medium was increased, the mole fraction of hv in the copolymer increased. when soy waste was used as a c-source a copolymer, a high hv mole fraction (hb:hv, 75:25) was produced while when malt waste was used, a much lower hv mole fraction (hb:hv, 90 ... | 2007 | 16844370 |
| biological treatment of industrial wastes in a photobioreactor. | an algal-bacterial consortium was tested for the treatment from a coke factory. a chlorella vulgaris strain and a phenol-degrading alcaligenes sp. were first isolated from the wastewater treatment plant to serve as inocula in the subsequent biodegradation tests. batch tests were then conducted with samples from the real wastewater or using a synthetic wastewater containing 325 mg phenol/l and 500 mg nh4+/l as target pollutants. direct biological treatment of the real wastewater was not possible ... | 2006 | 16862781 |
| improvement of the activity of arylmalonate decarboxylase by random mutagenesis. | arylmalonate decarboxylase (ec 4.1.1.76) catalyzes enantioselective decarboxylation of alpha-aryl-alpha-methylmalonates to give optically pure alpha-arylpropionates. recently, we have succeeded in creating a double mutant enzyme that gave opposite enantionmer as the product. unfortunately, however, the activity of the mutant decreased far lower than that of the native enzyme. thus, we performed the directed evolution of the mutant via the random mutagenesis method employing the mutator strain es ... | 2006 | 16865343 |
| bioremediation of soil contaminated with pentachlorophenol (pcp) using humic acids bound on zeolite. | we determined the toxicity of various chlorophenols, especially pentachlorophenol (pcp), on five bacterial strains and studied pcp biodegradation in soils amended with an organomineral complex (omc) prepared from humic acids (organic part) bound on zeolite (inorganic part). both components of omc have excellent sorption properties and are of natural origin and therefore suitable to be used in the environment. toxicity of chlorophenols depends not only on the number of chlorine atoms but also on ... | 2007 | 16876229 |
| effectiveness of biodegradation of petroleum products by mixed bacterial populations in liquid medium at different ph values. | the possibility of using mineral oils as a carbon source by bacteria adapted to high oil concentrations was tested in liquid media with different ph values (ph = 5, 7 and 9). two types of inocula were tested: inoculum i consisted of selected strains used in the bioremediation of oil-contaminated soils and inoculum ii contained bacteria isolated from soil samples previously bioremediated at ph = 5, 7 and 9. biodegradation was observed in all the investigated media independently of initial ph valu ... | 2006 | 16878607 |
| a comparative investigation of the thermal unfolding of pseudoazurin in the cu(ii)-holo and apo form. | the contribution of the copper ion to the stability and to the unfolding pathway of pseudoazurin was investigated by a comparative analysis of the thermal unfolding of the cu(ii)-holo and apo form of the protein. the unfolding has been followed by calorimetry, fluorescence, optical density, and electron paramagnetic resonance (epr) spectroscopy. the thermal transition of cu(ii)-holo pseudoazurin is irreversible and occurs between 60.0 and 67.3 degrees c, depending on the scan rate and technique ... | 2006 | 16881076 |
| piggery wastewater treatment using alcaligenes faecalis strain no. 4 with heterotrophic nitrification and aerobic denitrification. | alcaligenes faecalis strain no. 4, which has heterotrophic nitrification and aerobic denitrification abilities, was used to treat actual piggery wastewater containing high-strength ammonium under aerobic conditions. in a continuous experiment using a solids-free wastewater (sfw) mixed with feces, almost all of the 2000 nh4+ -n mg/l and 12,000 cod mg/l in the wastewater was removed and the ammonium removal rate was approximately 30 mg-n/l/h, which was 5-10 times higher than the rates achieved by ... | 2006 | 16893560 |
| identification of acetate- or methanol-assimilating bacteria under nitrate-reducing conditions by stable-isotope probing. | stable-isotope probing (sip) was used to identify acetate- or methanol-assimilating bacteria under nitrate-reducing conditions in activated sludge. a sludge sample obtained from wastewater treatment systems was incubated in a denitrifying batch reactor fed with synthetic wastewater containing [(13)c]acetate or [(13)c]methanol as the main carbon source and nitrate as the electron acceptor. we analyzed how growth of bacterial populations was stimulated by acetate or methanol as the external carbon ... | 2006 | 16897304 |
| effect of ph, temperature and substrate on n2o, no and co2 production by alcaligenes faecalis p. | to study the effect of ph, temperature and substrate on the magnitude of n(2)o and no production by heterotrophic nitrifiers. | 2006 | 16907816 |
| chemical resistance of the gram-negative bacteria to different sanitizers in a water purification system. | purified water for pharmaceutical purposes must be free of microbial contamination and pyrogens. even with the additional sanitary and disinfecting treatments applied to the system (sequential operational stages), pseudomonas aeruginosa, pseudomonas fluorescens, pseudomonas alcaligenes, pseudomonas picketti, flavobacterium aureum, acinetobacter lowffi and pseudomonas diminuta were isolated and identified from a thirteen-stage purification system. to evaluate the efficacy of the chemical agents u ... | 2006 | 16914053 |
| analysis of polyhydroxyalkanoate (pha) synthase gene in activated sludge that produces pha containing 3-hydroxy-2-methylvalerate. | the identification of phac which encodes pha synthase, that is involved in the formation of polyhydroxyalkanoate (pha) containing 3-hydroxy-2-methylvalerate (3h2mv), was attempted. as of now, pha containing 3h2mv has been reported to be produced only by mixed microbial cultures in activated sludge, but no pure bacterial cultures. a laboratory-scale activated sludge process was operated for 67 days. during the operation of the activated sludge process, its capacity to produce pha containing 3h2mv ... | 2007 | 16933327 |
| [in vitro adherence of staphylococcus epidermidis and pseudomonas alcaligenes to intraocular lenses]. | to quantify and compare the in vitro adherence of staphylococcus epidermidis and pseudomonas alcaligenes to different intraocular lenses (iols). | 2006 | 16936954 |
| biotransformation of isoeugenol to vanillin by pseudomonas putida ie27 cells. | the ability to produce vanillin and/or vanillic acid from isoeugenol was screened using resting cells of various bacteria. the vanillin- and/or vanillic-acid-producing activities were observed in strains belonging to the genera achromobacter, aeromonas, agrobacerium, alcaligenes, arthrobacter, bacillus, micrococcus, pseudomonas, rhodobacter, and rhodococcus. strain ie27, a soil isolate showing the highest vanillin-producing activity, was identified as pseudomonas putida. we optimized the culture ... | 2007 | 16944125 |
| post-ercp bacteremia caused by alcaligenes xylosoxidans in a patient with pancreas cancer. | alcaligenes xylosoxidans is an aerobic, motile, oxidase and catalase positive, nonfermentative gram negative bacillus. this bacterium has been isolated from intestine of humans and from various hospital or environmental water sources. a.xylosoxidans is both waterborne and results from the poor-hygienic conditions healthcare workers are in. in this case report, the bacteremia which appeared in a patient with pancreas cancer after ercp was described. | 2006 | 16945159 |
| fluorescence in situ hybridization for rapid identification of achromobacter xylosoxidans and alcaligenes faecalis recovered from cystic fibrosis patients. | achromobacter xylosoxidans is frequently isolated from the respiratory secretions of cystic fibrosis (cf) patients, but identification with biochemical tests is unreliable. we describe fluorescence in situ hybridization assays for the rapid identification of achromobacter xylosoxidans and alcaligenes faecalis. both assays showed high sensitivities and high specificities with a collection of 155 nonfermenters from cf patients. | 2006 | 16954289 |
| probing the unusual oxidation/reduction behavior of paracoccus pantotrophus cytochrome cd1 nitrite reductase by replacing a switchable methionine heme iron ligand with histidine. | a m106h variant, where m106 is a c-type heme iron axial ligand, of cytochrome cd(1) nitrite reductase is an inactive protein in vivo. expression of the holoprotein in paracoccus pantotrophus required generation of nitric oxide during cell growth through simultaneous expression of an exogenous copper nitrite reductase from alcaligenes faecalis. in the absence of the latter protein, only a semi-apo form of m106h cytochrome cd(1) was formed. thus it was demonstrated that expression of the chromosom ... | 2006 | 16964982 |
| the efficacy of postenucleation saline wash and the effect of different antimicrobial agents on microbial contamination of donor eyes. | to evaluate the efficacy of postenucleation saline wash and the effect of different antimicrobial agents on the microbial contamination of donor eyes. | 2006 | 16974130 |
| atomic level insight into the oxidative half-reaction of aromatic amine dehydrogenase. | the quinoprotein aromatic amine dehydrogenase (aadh) uses a covalently bound tryptophan tryptophylquinone (ttq) cofactor to oxidatively deaminate primary aromatic amines. recent crystal structures have provided insight into the reductive half-reaction. in contrast, no atomic details are available for the oxidative half-reaction. the ttq o7 hydroxyl group is protonated during reduction, but it is unclear how this proton can be removed during the oxidative half-reaction. furthermore, compared with ... | 2006 | 17005560 |
| catabolic pathways and biotechnological applications of microbial caffeine degradation. | catabolism of caffeine (1,3,7-trimethylxanthine) in microorganisms commences via two possible mechanisms: demethylation and oxidation. through the demethylation route, the major metabolite formed in fungi is theophylline (1,3-dimethylxanthine), whereas theobromine (3,7-dimethylxanthine) is the major metabolite in bacteria. in certain bacterial species, caffeine has also been oxidized directly to trimethyl uric acid in a single step. the conversion of caffeine to its metabolites is primarily brou ... | 2006 | 17009088 |
| selection of photosynthetic bacterium rhodobacter sphaeroides 14f for polyhydroxyalkanoate production with two-stage aerobic dark cultivation. | polyhydroxyalkanoate (pha) production abilities in a two-stage aerobic dark culture of photosynthetic bacteria were investigated at relatively high temperatures (37-40 degrees c). a 14f strain, identified as rhodobacter sphaeroides, showed the highest pha production (3.5 g/l pha with 60% pha content). its productivity was 2-3 times higher than those of other photosynthetic bacteria. | 2006 | 17027875 |
| intersite structural rearrangement of the blue copper site induced by substrate binding: spectroscopic studies of a copper-containing nitrite reductase from alcaligenes xylosoxidans ncimb 11015. | a copper-containing nitrite reductase from alcaligenes xylosoxidans ncimb 11015 has its own unique blue or type 1 copper protein resonance raman spectrum in the usual cu-s(cys) stretching region, nu(cu-s(cys)), with a pair of strong peaks at 412 and 420 cm(-1) and a weak peak at 364 cm(-1). the predominantly nu(cu-s(cys)) raman bands at 412, 420, and 364 cm(-1) of the type 1 copper site all shifted to higher frequencies upon binding of nitrite to the type 2 copper site, and the resonance raman d ... | 2006 | 17029353 |
| an active site tyrosine residue is essential for amidohydrolase but not for esterase activity of a class 2 histone deacetylase-like bacterial enzyme. | hdacs (histone deacetylases) are considered to be among the most important enzymes that regulate gene expression in eukaryotic cells acting through deacetylation of epsilon-acetyl-lysine residues within the n-terminal tail of core histones. in addition, both eukaryotic hdacs as well as their bacterial counterparts were reported to also act on non-histone targets. however, we are still far from a comprehensive understanding of the biological activities of this ancient class of enzymes. in the pre ... | 2007 | 17037985 |
| crystal structure of an electron transfer complex between aromatic amine dehydrogenase and azurin from alcaligenes faecalis. | the crystal structure of an electron transfer complex of aromatic amine dehydrogenase (aadh) and azurin is presented. electrons are transferred from the tryptophan tryptophylquinone (ttq) cofactor of aadh to the type i copper of the cupredoxin azurin. this structure is compared with the complex of the ttq-containing methylamine dehydrogenase (madh) and the cupredoxin amicyanin. despite significant similarities between the two quinoproteins and the two cupredoxins, each is specific for its respec ... | 2006 | 17087503 |
| [occurrence of giardia cysts and cryptosporidium oocysts in swimming pools in the province of palermo, italy]. | 21 samples of waters of 7 swimming pools in the province of palermo were investigated for the presence of giardia cysts and cryptosporidium oocysts. some chemio-physical parameters (chlorine, temperature, turbidity and ph) were also evaluated as well as the presence of bacterial indicators of faecal (total coliforms, escherichia coli and enterococci), mucocutaneous and environmental (staphylococcus aureus and pseudomonas aeruginosa) contamination. all samples were negative for bacterial indicato ... | 2006 | 17089952 |
| effect of surfactants on fluoranthene degradation by pseudomonas alcaligenes pa-10. | two surfactants, tween 80 and jbr, were investigated for their effect on fluoranthene degradation by a pseudomonad. both surfactants enhanced fluoranthene degradation by pseudomonas alcaligenes pa-10 in shake flask culture. this bacterium was capable of utilising the synthetic surfactant and the biosurfactant as growth substrates and the critical micelle concentration of neither compound inhibited bacterial growth. the biosurfactant jbr significantly increased polycyclic aromatic hydrocarbon (pa ... | 2007 | 17106676 |
| proteome analysis of heat shock protein expression in pseudomonas alcaligenes ncimb 9867 in response to gentisate exposure and elevated growth temperature. | pseudomonas alcaligenes ncimb 9867 (strain p25x) degrades xylenols and cresols via the gentisate pathway. p25x expresses two isofunctional gentisate 1,2-dioxygenases (gdo i and gdo ii). the expression of both gdos was not detected when p25x cells were grown at 42 degrees c, even in the presence of gentisate. a total of 19 heat shock proteins (hsps) belonging to the hsp100, hsp90, hsp70, hsp60, hsp45, and small heat shock protein (shsp) families were identified among the protein spots that were e ... | 2007 | 17149773 |
| molecular basis for nitric oxide dynamics and affinity with alcaligenes xylosoxidans cytochrome c. | the bacterial heme protein cytochrome ć from alcaligenes xylosoxidans (axcp) reacts with nitric oxide (no) to form a 5-coordinate ferrous nitrosyl heme complex. the crystal structure of ferrous nitrosyl axcp has previously revealed that no is bound in an unprecedented manner on the proximal side of the heme. to understand how the protein structure of axcp controls no dynamics, we performed absorption and raman time-resolved studies at the heme level as well as a molecular computational dynamics ... | 2007 | 17158883 |
| metabolism of 2-, 3- and 4-hydroxybenzoates by soil isolates alcaligenes sp. strain pph and pseudomonas sp. strain ppd. | pseudomonas sp. strain ppd and alcaligenes sp. strain pph isolated from soil by enrichment culture technique utilize 2-, 3- and 4-hydroxybenzoates as the sole source of carbon and energy. the degradation pathways were elucidated by performing whole-cell o(2) uptake, enzyme activity and induction studies. depending on the mixture of carbon source and the preculture condition, strain pph was found to degrade 2-hydroxybenzoate either via the catechol or gentisate route and has both salicylate 1-hyd ... | 2007 | 17169001 |
| hydrogen cyanide-producing rhizobacteria kill subterranean termite odontotermes obesus (rambur) by cyanide poisoning under in vitro conditions. | the subterranean termite odontotermes obesus is an important pest of the indian subcontinent, causing extensive damage to major agricultural crops and forest plantation trees. control of termites by strategies employing their parasites has limitations because they have evolved a complex social structure, immune responses, and adaptive behavior toward pathogen-infected individuals. nonparasitic rhizobacteria that produce harmful metabolites might facilitate the biocontrol of termites. in the pres ... | 2007 | 17171461 |
| [16s rdna-rflp analysis of structure and diversity of an aerobic microbial community degrading hexachlorobenzene]. | hexachlorobenzene is a chlorinated aromatic hydrocarbon that was widely used as a seed dressing for prevention of fungal growth on crops, and is also a component of fireworks, ammunition, and synthetic rubbers. because of the bioaccumulation and persistence of hexachlorobenzene as well as its potential toxicity, hexachlorobenzene must be removed from environment. the potential for aerobic dechlorination of hexachlorobenzene by a hexachlorobenzene-adapted mixed culture was investigated. an aerobi ... | 2006 | 17172024 |
| redox-state-dependent complex formation between pseudoazurin and nitrite reductase. | bacterial copper-containing nitrite reductase catalyzes the reduction of nitrite to nitric oxide as part of the denitrification process. pseudoazurin interacts with nitrite reductase in a transient fashion to supply the necessary electrons. the redox-state dependence of complex formation between pseudoazurin and nitrite reductase was studied by nuclear magnetic resonance spectroscopy and isothermal titration calorimetry. binding of pseudoazurin in the reduced state is characterized by the presen ... | 2007 | 17199303 |
| infection of a total hip prosthesis in a dog caused by achromobacter(alcaligenes) xylosoxidans. | a four-year-old male, neutered labrador retriever was presented with progressive left hindlimb lameness 10 months following total hip replacement. radiography revealed changes consistent with infection and culture of joint fluid from the left coxofemoral joint revealed achromobacter (alcaligenes) xylosoxidans. the prosthesis was removed. culture of the acetabular cup confirmed achromobacter xylosoxidans. achromobacter xylosoxidans is an uncommon but serious cause of nosocomial epidemics in hospi ... | 2006 | 17201829 |
| gentisate 1,2-dioxygenase from xanthobacter polyaromaticivorans 127w. | a putative gentisate 1,2-dioxygenase was encoded in the dibenzothiophene degradation gene cluster (dbd) from xanthobacter polyaromaticivorans 127w. the deduced amino acid sequence showed high sequence similarity with gentisate dioxygenases from pseudomonas alcaligenes (aad49427, 65% identical), bradyrhizobium japonicum (np_766750, 64%), and p. aeruginosa (zp_00135722, 54%), and moderate similarity with 1-hydroxy-2-naphthoate dioxygenase from nocardioides sp. kp7 (baa31235, 33%) and salicylate di ... | 2007 | 17213679 |
| histone deacetylase inhibitor assay based on fluorescence resonance energy transfer. | histone deacetylases (hdacs) are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. furthermore, in recent years hdacs occupied a major position as key targets for chemotherapeutic intervention in malignant diseases. however, progress in the development of these new chemotherapeutics is largely dependent on the existence of bioassays well-suited to inhibitor screening. herein, we present the first nonisotopic competition binding assay for hdacs. the assa ... | 2007 | 17250798 |
| species-specific functioning of the pseudomonas xcpq secretin: role for the c-terminal homology domain and lipopolysaccharide. | secretins are oligomeric proteins that mediate the export of macromolecules across the bacterial outer membrane. the members of the secretin superfamily possess a c-terminal homology domain that is important for oligomerization and channel formation, while their n-terminal halves are thought to be involved in system-specific interactions. the xcpq secretin of pseudomonas spp. is a component of the type ii secretion pathway. xcpq from pseudomonas alcaligenes is not able to functionally replace th ... | 2007 | 17277064 |
| microbiological changes in naturally fermented cassava fish (pseudotolithus sp.) for lanhouin production. | cassava fish (pseudotolithus sp.) was naturally fermented on three different occasions at room temperature (28-30 degrees c) for 3 to 8 days and the microbial population and the occurrence of various bacterial species were monitored. in general, after a slight increase during the early stages of fermentation, the microbial population decreased as the fermentation progressed. a total of 224 isolates belonging to the genera bacillus, staphylococcus, micrococcus, streptococcus, corynebacterim, pseu ... | 2007 | 17291615 |
| bioaugmented sulfur-oxidizing denitrification system with alcaligenes defragrans b21 for high nitrate containing wastewater treatment. | the performance of enriched sludge augmented with the b21 strain of alcaligenes defragrans was compared with that of enriched sludge, as well as with pure alcaligenes defragrans b21, in the context of a sulfur-oxidizing denitrification (sod) process. in synthetic wastewater treatment containing 100-1,000 mg no3-n/l, the single strain-seeded system exhibited superior performance, featuring higher efficiency and a shorter startup period, provided nitrate loading rate was less than 0.2 kg no3-n/m(3 ... | 2007 | 17294220 |
| improvement in ammonium removal efficiency in wastewater treatment by mixed culture of alcaligenes faecalis no. 4 and l1. | to improve ammonium removal efficiency in wastewater treatment, a mixed culture of alcaligenes faecalis no. 4 and its mutant l1, both of which have heterotrophic nitrification and aerobic denitrification abilities, was performed. in a batch culture, no. 4 has a higher denitrification ability than l1, but its ammonium removal rate was lower. in a mixed continuous culture in the ammonium loading range of 750 to 3500 mg-n/l/d, the average ammonium removal rate and the average denitrification ratio ... | 2007 | 17298903 |
| [isolation, degradation and phylogenetic analysis of methylparathion degradative strain x4]. | the strain x4 was isolated from polluted wastes of shandong huayang pesticide chemical industry group co., ltd. it was cultured with methylparathion and p-nitrophenol, a kind of intermediate during methylparathion degrading process, and used them as the sole carbon source and decomposed them into carbon dioxide and wastes. it is identified as arthrobacter sp. by means of a series of tests, and it has visible sphere-pole variable cycle. it is bacilliform when it is cultured in nutrient broth medi ... | 2006 | 17302165 |
| decolorisation and detoxification of direct blue-15 by a bacterial consortium. | studies were carried out on decolorisation and biotransformation of the dye direct blue-15 into 3,3'-dimethoxybenzidine (o'-dianisidine) and a sulphonated derivative by a five-member bacterial consortium. chromatographic studies revealed further complete biodegradation of 3,3'-dimethoxybenzidine coupled with release of ammonia, but the recalcitrant sulphonated derivative persisted. the microorganisms identified in the mixed consortium by 16s rdna sequence analysis were alcaligenes faecalis, sphi ... | 2007 | 17321737 |
| choline biosensor constructed with chitinous membrane from soldier crab and its application in measuring cholinesterase inhibitory activities. | an amperometric flow-injection choline biosensor was assembled utilizing natural chitinous membrane as the supporting material for biocatalyst immobilization, and the membrane was purified from taiwanese soldier crab, mictyris brevidactylus. the chitinous membrane (<50.0 microm in thickness) was covalently immobilized with choline oxidase (ec 3.1.1.17 from alcaligenes sp.) and then attached onto the platinum electrode of an amperometric flow cell. the flow cell served as the choline sensing devi ... | 2007 | 17329059 |
| solar-based detoxification of phenol and p-nitrophenol by sequential tio2 photocatalysis and photosynthetically aerated biological treatment. | simulated solar uv/tio(2) photocatalysis was efficient to detoxify a mixture of 100 mgphenoll(-1) and 50 mgp-nitrophenol (pnp) l(-1) and allow the subsequent biodegradation of the remaining pollutants and their photocatalytic products under photosynthetic aeration with chlorella vulgaris. photocatalytic degradation of phenol and pnp was well described by pseudo-first order kinetics (r(2)>0.98) with removal rate constants of 1.9x10(-4) and 2.8x10(-4)min(-1), respectively, when the pollutants were ... | 2007 | 17350074 |
| salvage treatment of pneumonia and initial treatment of tracheobronchitis caused by multidrug-resistant gram-negative bacilli with inhaled polymyxin b. | systemic colistin has shown efficacy against multidrug-resistant pseudomonas aeruginosa and acinetobacter spp., but it has presented poor results in pneumonia. aerosolized polymyxin in cystic fibrosis patients has had good results. in this study, inhaled polymyxin b was used to treat respiratory infections by multidrug-resistant gram-negative bacilli (mr-gnbs). nineteen patients were treated with inhaled polymyxin b: 14 pneumonia, most of which had previously failed treatment with intravenous po ... | 2007 | 17350201 |
| development of a biosensor for caffeine. | we have utilized a microbe, which can degrade caffeine to develop an amperometric biosensor for determination of caffeine in solutions. whole cells of pseudomonas alcaligenes mtcc 5264 having the capability to degrade caffeine were immobilized on a cellophane membrane with a molecular weight cut off (mwco) of 3000-6000 by covalent crosslinking method using glutaraledhyde as the bifunctional crosslinking agent and gelatin as the protein based stabilizing agent (pbsa). the biosensor system was abl ... | 2007 | 17386510 |
| complex structure of a bacterial class 2 histone deacetylase homologue with a trifluoromethylketone inhibitor. | histone deacetylases (hdacs) have emerged as attractive targets in anticancer drug development. to date, a number of hdac inhibitors have been developed and most of them are hydroxamic acid derivatives, typified by suberoylanilide hydroxamic acid (saha). not surprisingly, structural information that can greatly enhance the design of novel hdac inhibitors is so far only available for hydroxamic acids in complex with hdac or hdac-like enzymes. here, the first structure of an enzyme complex with a ... | 2007 | 17401192 |
| metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake. | in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ... | 2007 | 17406774 |
| [reaction of microorganisms to the digestive fluid of the earthworms]. | the reaction of soil bacteria and fungi to the digestive fluid of the earthworm aporrectodea caliginosa was studied. the fluid was obtained by centrifugation of the native enzymes of the digestive tract. the inhibition of growth of certain bacteria, spores, and fungal hyphae under the effect of extracts from the anterior and middle sections of the digestive tract of a. caliginosa was discovered for the first time. in bacteria, microcolony formation was inhibited as early as 20-30 s after the app ... | 2007 | 17410875 |
| bacterial decolorization of acid orange 7 in the presence of ionic and non-ionic surfactants. | the effects of the non-ionic surfactant triton x-100, the cationic surfactant cetyltri-methylammonium bromide (ctab) and the anionic surfactant sodium n-lauroyl sarcosinate (sls) on the decolorization of the reaction medium containing the monoazo dye acid orange 7 (ao7) by alcaligenes faecalis and rhodococcus erythropolis were studied. it was found that the surfactants influenced in different ways the rate of decolorization. at all concentrations tested the non-ionic surfactant triton x-100 decr ... | 2007 | 17425112 |
| use of headspace solid-phase microextraction for the quantification of poly(3-hydroxybutyrate) in microbial cells. | biopolymers such as poly(hydroxyalkanoates) (phas) have received much attention due to their physico-chemical properties, biodegradability, and biocompatibility that make them good candidates for industrial and medical applications. produced by some microorganisms phas accumulate within the cells of these organisms. the optimization of microbial processes to produce phas at a lower cost requires rapid and accurate techniques for quantification of the biopolymer in biomass. the present study desc ... | 2007 | 17462661 |
| rapid microwave assisted esterification method for the analysis of poly-3-hydroxybutyrate in alcaligenes latus by gas chromatography. | in the present study, we used microwave energy instead of conventional heating to transform poly-3-hydroxybutyrate (phb) into methyl 3-hydroxybutyrate (me-3hb) in acidified methanol (h2so4, 10%, v/v) mixture in less than 4 min at 10% microwave power. the microwave assisted method was then applied to analyze phb produced by alcaligenes latus. the phb content in the biomass determined using microwave heating was comparable to the amount found by conventional heating. moreover, the new esterificati ... | 2007 | 17466318 |
| successful treatment and limb salvage of mucor necrotizing fasciitis after kidney transplantation with posaconazole. | this is a case of mucormycosis complicated by necrotizing fasciitis in a renal transplant recipient on immunosuppressive therapy treated with posaconazole. mucormycosis occurs most commonly as an opportunistic infection in the immunocompromised host. this patient, with predisposing risk factors for infection, including diabetes mellitus status post cadaveric renal transplantation on immunosuppressive therapy, is the first reported case of successful treatment of mucor involving an extremity whic ... | 2007 | 17472045 |
| new insights into the reductive half-reaction mechanism of aromatic amine dehydrogenase revealed by reaction with carbinolamine substrates. | aromatic amine dehydrogenase uses a tryptophan tryptophylquinone (ttq) cofactor to oxidatively deaminate primary aromatic amines. in the reductive half-reaction, a proton is transferred from the substrate c1 to betaasp-128 o-2, in a reaction that proceeds by h-tunneling. using solution studies, kinetic crystallography, and computational simulation we show that the mechanism of oxidation of aromatic carbinolamines is similar to amine oxidation, but that carbinolamine oxidation occurs at a substan ... | 2007 | 17475620 |
| association of bacteria with metacercarial cysts of echinostoma caproni. | echinostoma caproni metacercarial cysts often need to be stored for several months to a year to meet laboratory requirements. this study examined the bacteria associated with encysted metacercariae stored at 4 degrees c. bacterial assemblages from the kidney-pericardial region of uninfected snails and assemblages from the site of metacercarial infection in biomphalaria glabrata were determined. four species of gram-negative rods were isolated and identified from the stored cysts to species or ge ... | 2007 | 17483963 |
| the structure of the met144leu mutant of copper nitrite reductase from alcaligenes xylosoxidans provides the first glimpse of a protein-protein complex with azurin ii. | cu-containing nitrite reductases (nirs) perform the reduction of nitrite to no via an ordered mechanism in which the delivery of a proton and an electron to the catalytic type 2 cu site is highly orchestrated. electron transfer from a redox partner protein, azurin or pseudoazurin, to the type 1 cu site is assumed to occur through the formation of a protein-protein complex. we report here a new crystal form in space group p2(1)2(1)2(1) of the met144leu mutant of nir from alcaligenes xylosoxidans ... | 2007 | 17503096 |
| posttraumatic endophthalmitis due to a rare pathogen, alcaligenes faecalis. | 2007 | 17508059 | |
| gene cloning, expression, and substrate specificity of an imidase from the strain pseudomonas putida yz-26. | a gene-encoding imidase was isolated from pseudomonas putdia yz-26 genomic dna using a combination of polymerase chain reaction and activity screening the recombinant. analysis of the nucleotide sequence revealed that an open reading frame (orf) of 879 bp encoded a protein of 293 amino acids with a calculated molecular weight of 33712.6 kda. the deduced amino-acid sequence showed 78% identity with the imidase from alcaligenes eutrophus 112r4 and 80% identity with n-terminal 20 amino-acid imidase ... | 2007 | 17534563 |
| formation of amyloid fibrils via longitudinal growth of oligomers. | mature amyloid fibrils are believed to be formed by the lateral association of discrete structural units designated as protofibrils, but this lateral association of protofibrils has never been directly observed. we have recently characterized a thioesterase from alcaligenes faecalis, which was shown to exist as homomeric oligomers with an average diameter of 21.6 nm consisting of 22 kda subunits in predominantly beta-sheet structure. in this study, we have shown that upon incubation in a 75% eth ... | 2007 | 17536835 |
| oxidation of butane to butanol coupled to electrochemical redox reaction of nad+/nadh. | a crude cell extract from a butane-utilizing bacterium, alcaligenes sp., catalyzed the oxidation of butane to butanol coupled to nadh. a graphite electrode modified with neutral red (nr-electrode) catalyzed the reduction of nad(+) to nadh. about 4.9 mm butanol was produced from 50% n-butane/o(2) mixture through the combined reactions of the crude enzyme and the nr-electrode in 250 ml reactor for 3 h. | 2007 | 17549436 |
| emerging and unusual gram-negative infections in cystic fibrosis. | people with cystic fibrosis (cf) have chronic airway infection and frequent exposure to antibiotics, which often leads to the emergence of resistant organisms. in addition to the development of multiresistance in common cf pathogens such as pseudomonas aeruginosa, several newer, inherently resistant gram-negative species are becoming more common, including burkholderia cepacia complex, stenotrophomonas maltophilia, achromobacter (alcaligenes) xylosoxidans, certain ralstonia species, and those wi ... | 2007 | 17562501 |
| protein film voltammetry of copper-containing nitrite reductase reveals reversible inactivation. | the cu-containing nitrite reductase from alcaligenes faecalis s-6 catalyzes the one-electron reduction of nitrite to nitric oxide (no). electrons enter the enzyme at the so-called type-1 cu site and are then transferred internally to the catalytic type-2 cu site. protein film voltammetry experiments were carried out to obtain detailed information about the catalytic cycle. the homotrimeric structure of the enzyme is reflected in a distribution of the heterogeneous electron-transfer rates around ... | 2007 | 17579406 |
| [electron microscope investigation of the biofilms of a binary bacterial community]. | 2007 | 17583226 | |
| biodegradation of phenol at high initial concentration by alcaligenes faecalis. | strain alcaligenes faecalis was isolated and identified as a member of the genus alcaligenes by using biolog and 16s rdna sequence analysis. the phenol biodegradation tests showed that the phenol-degrading potential of a. faecalis related greatly to the different physiological phases of inoculum. the maximum phenol degradation occurred at the late phase of the exponential growth stages, where 1600 mg l(-1) phenol was completely degraded within 76 h. a. faecalis secreted and accumulated a vast qu ... | 2007 | 17597295 |
| cultivable bacterial diversity of alkaline lonar lake, india. | aerobic, alkaliphilic bacteria were isolated and characterized from water and sediment samples collected in the winter season, january 2002 from alkaline lonar lake, india, having ph 10.5. the total number of microorganisms in the sediment and water samples was found to be 10(2)-10(6) cfu g(-1) and 10(2)-10(4) cfu ml(-1), respectively. one hundred and ninety-six strains were isolated using different enrichment media. to study the bacterial diversity of lonar lake and to select the bacterial stra ... | 2008 | 17604989 |
| molecular characterization of a novel ortho-nitrophenol catabolic gene cluster in alcaligenes sp. strain nyz215. | alcaligenes sp. strain nyz215 was isolated for its ability to grow on ortho-nitrophenol (onp) as the sole source of carbon, nitrogen, and energy and was shown to degrade onp via a catechol ortho-cleavage pathway. a 10,152-bp dna fragment extending from a conserved region of the catechol 1,2-dioxygenase gene was obtained by genome walking. of seven complete open reading frames deduced from this fragment, three (onpabc) have been shown to encode the enzymes involved in the initial reactions of onp ... | 2007 | 17616586 |
| biodegradability of chlorinated anilines in waters. | to identify the bacteria tolerating chlorinated anilines and to study the biodegradability of o-chloroaniline and its coexistent compounds. | 2007 | 17624189 |
| inhibitor-mediated stabilization of the conformational structure of a histone deacetylase-like amidohydrolase. | histone deacetylases are major regulators of eukaryotic gene expression. not unexpectedly, histone deacetylases are among the most promising targets in cancer therapy. however, despite huge efforts in histone deacetylase inhibitor design, very little is known about the impact of histone deacetylase inhibitors on enzyme stability. in this study, the conformational stability of a well-established histone deacetylase homolog with high structural similarity (histone deacetylase-like amidohydrolase f ... | 2007 | 17627667 |
| alcaligenes (brucella) bronchisepticus: as a factor in porcine pneumonias. | 1943 | 17647930 |