Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| silencing of integrated human papillomavirus type 18 oncogene transcription in cells expressing serpinb2. | the serine protease inhibitor serpinb2 (pai-2), a major product of differentiating squamous epithelial cells, has recently been shown to bind and protect the retinoblastoma protein (rb) from degradation. in human papillomavirus type 18 (hpv-18)-transformed epithelial cells the expression of the e6 and e7 oncoproteins is controlled by the hpv-18 upstream regulatory region (urr). here we illustrate that pai-2 expression in the hpv-18-transformed cervical carcinoma line hela resulted in the restora ... | 2005 | 15767426 |
| [association between high-risk human papillomavirus dna load and cervical intraepithelial lesion]. | to determine the association between viral load of high risk type human papillomavirus (hr-hpv) and stage of cervical intraepithelial neoplasia (cin) lesion. | 2004 | 15769315 |
| the role of viral integration in the development of cervical cancer. | the development of invasive cervical cancer is associated with human papillomavirus (hpv) infection and subsequent integration into the host epithelium. more than 99% of cervical cancers contain hpv sequences, and many of these contain a truncated hpv genome integrated into a single position within the host genome. studies examining the role of viral integration in cervical cancer development have found that the sites of integration appear randomly distributed throughout the genome. this, and th ... | 2005 | 15771901 |
| 1h and 15n resonance assignment, secondary structure and dynamic behaviour of the c-terminal domain of human papillomavirus oncoprotein e6. | e6 is a viral oncoprotein implicated in cervical cancers, produced by human papillomaviruses (hpvs). e6 contains two putative zinc-binding domains of about 75 residues each. the difficulty in producing recombinant e6 has long hindered the obtention of structural data. recently, we described the expression and purification of e6-c 4c/4s, a stable, folded mutant of the c-terminal domain of hpv16 e6. here, we have produced 15n-labelled samples of e6-c 4c/4s for structural studies by nmr. we have as ... | 2005 | 15772752 |
| absence of human papillomavirus in esophageal carcinomas from southwestern kenya. | esophageal squamous cell cancer is highly prevalent in south-western kenya. the role of human papillomavirus (hpv) in esophageal cancers from this region was evaluated. biopsies of 29 esophageal squamous cell cancers were assayed for hpv dna sequences by reverse line blot polymerase chain reaction, using 27 hpv type-specific probes. viral sequences were found in none of the specimens. these results suggest the hpv is unlikely to be an etiologic factor for esophageal squamous cell cancers in this ... | 2005 | 15773838 |
| human papillomavirus genotypes and their association with cervical neoplasia in a cohort of western australian women. | human papillomavirus (hpv) is known to be the cause of almost all cervical cancers. the genotypes have been classified into high and low risk types according to their oncogenic potential. however, data for many of the genotypes are limited and some (hpv-26, 53, and 66) have no agreed status. a study was undertaken to determine the hpv genotype distribution in women of western australia and the association with cervical neoplasia. liquid based cervical samples from a cohort of 282 western austral ... | 2005 | 15778980 |
| after the promise: the std consequences of adolescent virginity pledges. | to examine the effectiveness of virginity pledges in reducing std infection rates among young adults (ages 18-24). | 2005 | 15780782 |
| detection of persistent high risk human papillomavirus infections with hybrid capture ii and spf10/lipa. | hpv infection in young women is common. however only a certain number of hpv genotypes are oncogenic. it is necessary for high risk hpv infection to persist at the cervix for a considerable time before oncogenesis occurs. | 2005 | 15780805 |
| opposing effects of bovine papillomavirus type 1 e6 and e7 genes on fas-mediated apoptosis. | programmed cell death (pcd), best exemplified by apoptosis, is a genetically programmed process of cellular destruction that is indispensable for normal development and homeostasis of multicellular organisms. tumor necrosis factor alpha (tnf) and related cytokines are employed by host defenses to eliminate virally infected cells through induction of apoptosis. many viruses have evolved specific gene products to modulate this process. we have recently shown that the bovine papillomavirus type 1 ( ... | 2005 | 15782122 |
| immunotherapeutic efficacy of vaccines generated by fusion of dendritic cells and hpv16-associated tumour cells. | utilization of vaccines generated by fusion of dendritic cells and tumour cells is a promising approach to tumour immunotherapy. we have examined the therapeutic efficacy of vaccines generated by fusion of hpv16-associated tumour cells tc-1 with syngeneic and allogeneic dendritic cells. locally administered hybrid cells generated by fusion of mhc class i+ tc-1 cells and syngeneic dc inhibited the growth of mhc class i+ tc-1 tumours, but not the growth of mhc class i- tc-1/a9-derived tumours. the ... | 2005 | 15783088 |
| incidence of cervical squamous intraepithelial lesions associated with hiv serostatus, cd4 cell counts, and human papillomavirus test results. | recent cervical cancer screening guidelines state that the interval between screenings can be safely extended to 3 years in healthy women 30 years or older who have normal cytology results and have negative test results for oncogenic human papillomavirus (hpv) dna. | 2005 | 15784870 |
| is p16(ink4a) expression more useful than human papillomavirus test to determine the outcome of atypical squamous cells of undetermined significance-categorized pap smear? a comparative analysis using abnormal cervical smears with follow-up biopsies. | to correlate high-risk human papillomavirus (hr-hpv) viral load to p16(ink4a) expression in atypical squamous cells of undetermined significance (ascus)-categorized pap smears with follow-up biopsies in order to elucidate their relationships in gynecological pathology. | 2005 | 15790434 |
| vascular endothelial growth factor (vegf) up-regulates epidermal growth factor receptor (egf-r) in cervical cancer in vitro: this action is mediated through hpv-e6 in hpv-positive cancers. | epidermal growth factor receptor (egf-r) up-regulation in cervical cancer cells leads to an increase in cell proliferative insulin-like growth factor ii (igf-ii) and vascular endothelial growth factor (vegf) and a decrease of the anti-proliferative igf-binding protein-3 (igf-bp3). the objectives for this study are: (a) to find if vegf, in turn, up-regulates egf-r and down-regulates igf-bp3; (b) to determine if human papilloma virus (hpv-e6) mediates this action of vegf in hpv-positive cells; and ... | 2005 | 15790460 |
| parallel nanoliter detection of cancer markers using polymer microchips. | a general multipurpose microchip technology platform for point-of-care diagnostics has been developed. real-time nucleic acid sequence-based amplification (nasba) for detection of artificial human papilloma virus (hpv) 16 sequences and siha cell line samples was successfully performed in cyclic olefin copolymer (coc) microchips, incorporating supply channels and parallel reaction channels. samples were distributed into 10 parallel reaction channels, and signals were simultaneously detected in 80 ... | 2005 | 15791339 |
| antiviral activity of cidofovir on a naturally human papillomavirus-16 infected squamous cell carcinoma of the head and neck (scchn) cell line improves radiation sensitivity. | high risk hpv types 16 and 18 are associated with cervical cancer and squamous cell carcinoma of the head and neck (scchn). cidofovir is an antiviral drug used to treat hpv-induced laryngeal papillomatosis and other viral infections, with initial reports suggesting activity in cervical carcinoma cells. we investigated the effects of cidofovir on a naturally hpv-16-transformed scchn cell line (upci:scc090), in comparison with a cervical carcinoma cell line (caski) of similar viral characteristics ... | 2005 | 15792615 |
| the mitotic chromosome binding activity of the papillomavirus e2 protein correlates with interaction with the cellular chromosomal protein, brd4. | the papillomavirus transcriptional activator, e2, is involved in key functions of the viral life cycle. these include transcriptional regulation, viral dna replication, and viral genome segregation. the transactivation domain of e2 is required for each of these functions. to identify the regions of the domain that mediate binding to mitotic chromosomes, a panel of mutations has been generated and their effect on various e2 functions has been analyzed. a structural model of the bovine papillomavi ... | 2005 | 15795266 |
| induction of the human papillomavirus type 31 late promoter requires differentiation but not dna amplification. | the human papillomavirus (hpv) life cycle is linked to the differentiation state of the host cell. in virus-infected undifferentiated basal epithelial cells, hpv genomes are maintained as episomes at low copy number. upon differentiation, a concomitant increase in viral copy number and an induction of late gene expression from a differentiation-specific promoter is seen. to investigate whether late gene expression was dependent on the amplification of the viral genome, inhibitors of dna replicat ... | 2005 | 15795277 |
| adenoviral p53 effects and cell-specific e7 protein-protein interactions of human cervical cancer cells. | we investigated the time-course tumor growth suppression effects of recombinant adenovirus expressing p53 on human cervical cancer cells and cell-specific e7 protein-protein interactions in cell lysates using surface plasmon resonance (spr) biosensor. six hpv-infected human cervical cancer cell lines (hpv 16-positive cells, caski and siha cells; hpv 18-positive cells, hela and helas3 cells; and hpv negative c33a and ht3 cells) were used. after infection with adcmvp53, the cell-specific growth in ... | 2005 | 15797321 |
| immunohistochemical expression of p16ink4a and bcl-2 according to hpv type and to the progression of cervical squamous intraepithelial lesions. | inactivation of the cell cycle inhibitor gene p16mts1 seems to be involved in human papillomavirus (hpv)-related carcinogenesis because e6 and e7 oncoproteins may impair p16ink4a and, indirectly, bcl-2 functions. in this study, we analyzed the role of immunohistochemical expression of p16ink4a and bcl-2 in hpv-infected cervical biopsies as prognostic markers of the progression of squamous intraepithelial lesion (sil). sixty-five cervical biopsies were stratified into two subgroups according to t ... | 2005 | 15805425 |
| hpv type concordance in sexual couples determines the effect of condoms on regression of flat penile lesions. | we earlier demonstrated, in a randomised clinical trial, that the regression time of flat penile lesions in male sexual partners of women with cervical intraepithelial neoplasia (cin) was shorter in men who used condoms compared to those who did not. to further evaluate this finding, we examined whether the effect of condom use on the regression of flat penile lesions depends on the presence of human papillomavirus (hpv) type concordance in sexual couples, as determined in cervical and penile sc ... | 2005 | 15812547 |
| inhibitory effect of jaceosidin isolated from artemisiaargyi on the function of e6 and e7 oncoproteins of hpv 16. | jaceosidin (4',5,7-trihydroxy-3',6-dimethoxyflavone) was isolated from artemisia argyi as a putative oncogene inhibitor. jaceosidin inhibited binding between oncoprotein e6 of the human papillomavirus and the p53 tumor suppressor protein. in addition, jaceosidin inhibited binding between the e7 oncoprotein and the rb tumor suppressor protein, and also inhibited the function of hpv-16 harboring cervical cancer cells, including siha and caski. collectively, jaceosidin inhibited the functions of th ... | 2005 | 15814270 |
| family history as a co-factor for adenocarcinoma and squamous cell carcinoma of the uterine cervix: results from two studies conducted in costa rica and the united states. | previous work suggests that cervical cancer may aggregate in families. we evaluated the association between a family history of gynecological tumors and risk of squamous cell and adenocarcinomas of the cervix in 2 studies conducted in costa rica and the united states. the costa rican study consisted of 2,073 women (85 diagnosed with cin3 or cancer, 55 diagnosed with cin2 and 1,933 controls) selected from a population-based study of 10,049 women. the u.s. study consisted of 570 women (124 with in ... | 2005 | 15818615 |
| induction of human papillomavirus type 16-specific immunologic responses in a normal and an human papillomavirus-infected populations. | human papillomavirus (hpv) infection, especially with the oncogenic genotypes, is the most important risk factor for developing cervical cancer. we focused on generating hpv16 e7-specific cytotoxic cd8(+) t lymphocytes and evaluating hpv16 e7-specific immune responses in hpv16-infected and uninfected populations. peripheral blood mononuclear cells (pbmcs) were first collected from an uninfected group with an human lymphocyte antigen (hla) a2 haplotype (four volunteers). mature monocyte-derived d ... | 2005 | 15819706 |
| human papillomavirus genome detection by in situ hybridization in fine-needle aspirates of metastatic lesions from head and neck squamous cell carcinomas. | patients with head and neck squamous cell carcinoma (hnscc) often present with metastatic disease. the diagnosis of metastatic lesions usually is determined by fine-needle aspiration. human papillomavirus (hpv) is now being considered as a causative agent in a subset of hnscc. the objectives of this study were, first; to search for the presence of hpv dna by in situ hybridization (ish) in metastatic lesions from hnscc using alcohol-fixed, archival, cytopathologic material; second, to characteriz ... | 2005 | 15822131 |
| human papillomavirus status in advanced cervical cancer: predictive and prognostic significance for curative radiation treatment. | human papillomavirus (hpv) infection plays a major role in oncogenesis of squamous cell carcinoma of the cervix. this study was performed to investigate if hpv status and e2 gene integrity are prognostic parameters for clinical outcome and predictive for radiation response. forty women with locally advanced cervical cancer treated with curative radiotherapy were analyzed for hpv infection and e2 gene integrity by multiplex polymerase chain reaction. statistical analyses were performed for overal ... | 2005 | 15823112 |
| a case with multiple gynecological malignancies. | a patient with cervical non-hodgkin lymphoma was treated with chemotherapy. fourteen months after the diagnosis of the lymphoma, an endometrial adenocarcinoma was detected as a secondary malignant tumor. the patient was treated with surgery followed by radiotherapy. approximately 7 years after the diagnosis of endometrial cancer, vaginal invasive squamous cell carcinoma was diagnosed as the third primary malignancy, and a second-line palliative radiotherapy was applied. seven months after the la ... | 2005 | 15823128 |
| human papillomaviruses in lymph node neck metastases of head and neck cancers. | the results of this study corroborate earlier findings that human papillomavirus (hpv)16 is the most prevalent type of hpv in squamous cell carcinomas of the head and neck (scchns) and reinforce a possible influence of hpv on scchn progression by showing that the majority of hpv-positive patients harbor hpv16 (or hpv33) both in their primary tumors and in lymph node neck metastases (lnnms). | 2005 | 15823814 |
| cell-surface display of e7 antigen from human papillomavirus type-16 in lactococcus lactis and in lactobacillus plantarum using a new cell-wall anchor from lactobacilli. | the human papillomavirus type-16 (hpv-16) e7 protein is considered a major viral oncoprotein involved in cervical cancer (cxca) and a potential candidate for the development of a vaccine against this neoplasia. here, two lactic acid bacteria (the model one lactococcus lactis and a probiotic one lactobacillus plantarum) were engineered to deliver an e7 mutant protein (e7mm), which has a reduced transforming activity and consequently, could fit better to therapeutic use in humans than the native f ... | 2005 | 15823960 |
| human papillomavirus 16 virus-like particles use heparan sulfates to bind dendritic cells and colocalize with langerin in langerhans cells. | langerhans cells (lc), the immature dendritic cells (dc) that reside in epithelial tissues are among the first immune cells to encounter human papillomavirus (hpv) and are not activated by hpv virus-like particles (vlps) in contrast to dc. the notion that the differences in response to hpv vlps between lc and dc are associated with different types of cell binding and intracellular trafficking has been addressed. inhibition experiments with heparin and sodium chlorate showed that heparan sulfates ... | 2005 | 15831940 |
| proteomic analysis of progressive factors in uterine cervical cancer. | human papillomavirus (hpv) infections play a crucial role in the progress of cervical cancer. the high-risk hpv types are frequently associated with the development of malignant lesions. some of the latest studies have demonstrated that the high-risk hpv 16 and 18 are predominantly detected in the more aggressive cancers. in the present study, we aimed to establish the proteomic profiles and characterization of the tumor related proteins by using two-dimensional gel electrophoresis (2-de) and ma ... | 2005 | 15838902 |
| genital human papillomavirus testing by in situ hybridization in liquid atypical cytologic materials and follow-up biopsies. | to describe cases of hpv testing by dna in situ hybridization performed on atypical cervicovaginal samples collected by a liquidsed method that were negative for hpv dna on cytology but revealed cervical intraepithelial neoplasia on follow-up biopsies. | 2005 | 15839614 |
| study of the viral infections and cytokines associated with recurrent aphthous ulceration. | mouth ulcers are one of the most common oral complaints. however, the association between oral ulceration and viruses and cytokines is uncertain. we detected the presence of human papilloma virus (hpv), cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv)-1, hsv-2 and human herpesvirus (hhv)-8 dna in oral tissues by polymerase chain reaction (pcr) and southern hybridization techniques, and quantified the serum levels of cytokines including interleukin (il)-2, il-4, granulo ... | 2005 | 15840465 |
| [antigen retrieval immunohistochemistry under the influence of ph value and time]. | to find the best antigen retrieval buffer and retrieval time for the immunohistochemical staining of p504s, p63, cd10, and ki-67. | 2005 | 15841154 |
| a novel filtration-based processing method of liquid cytology specimens for human papillomavirus dna testing by hybrid capture ii. | we evaluated a more efficient method of processing liquid-based cervical cytology specimens for human papillomavirus (hpv) dna testing by hybrid capture ii (hcii). aliquots were made from 701 specimens in the following sequence: 4.0, 2.0, 1.0, 0.5, and 1.5 ml. the 4.0-ml aliquot was processed by the standard method (stp), and half of the processed material was tested by hcii. other aliquots were processed with a new, filtration-based processing method (npm). the 2.0-ml npm aliquot had hcii test ... | 2005 | 15842050 |
| human papillomavirus anogenital disease in hiv-infected individuals. | human papillomavirus (hpv) is one of the most common sexually transmitted infections and a significant cause of anogenital malignancies, precancer lesions, and cutaneous disease. human immunodeficiency virus (hiv)-positive individuals have a higher prevalence of hpv infection and hpv-associated anogenital disease compared to age-matched hiv-negative controls. data suggest that there has been little reduction in hpv-associated disease since the introduction of highly active antiretroviral therapy ... | 2005 | 15842614 |
| e7-expressing hacat keratinocyte cells are resistant to oxidative stress-induced cell death via the induction of catalase. | cervical carcinoma is one of the most prevalent cancers in women worldwide, and human papillomavirus (hpv) type 16 is the most common agent linked to human cervical carcinoma. in order to identify various relevant factors affected by the e7 oncogene, we established a stable cell line, which constitutively expressed e7 using the hacat human keratinocyte cell line. the increased expression and activity of catalase in the e7-expressing hacat cells (hacat/e7) were verified via matrix-assisted laser ... | 2005 | 15852342 |
| hpv-16 l1 vlp vaccine elicits a broad-spectrum of cytokine responses in whole blood. | here, we evaluated innate and adaptive immune system cytokine responses induced by hpv-16 l1 vlp in whole blood (wb) cultures from individuals receiving the vaccine (n=20) or placebo (n=4) before and after vaccination. 11 cytokines were measured: il-1beta, il-2, il-4, il-5, il-6, il-8, il-10, il-12, ifn-gamma, tnf-alpha, and gm-csf using multiplex bead arrays. cytokine profiles from wb samples clearly discriminated between vaccine and placebo recipients and between pre and post-vaccination respo ... | 2005 | 15855014 |
| human papillomavirus type 16 e6 and e7 oncoproteins upregulate c-iap2 gene expression and confer resistance to apoptosis. | inhibition of apoptosis plays an important role in the cellular immortalization and transformation induced by e6 and e7 oncoproteins of human papillomavirus (hpv). here, we report that the transcription of the inhibitor of apoptosis gene, cellular inhibitor of apoptosis protein 2, (c-iap2), is significantly upregulated in hpv16 e6/e7-immortalized human oral keratinocytes (hok16e6e7). overexpression of e6/e7 from the high-risk hpv16 or 18, but not from the low-risk hpv6, activated c-iap2 promoter ... | 2005 | 15856013 |
| marginal periodontium as a potential reservoir of human papillomavirus in oral mucosa. | although human papillomaviruses (hpvs) are associated with a number of proliferative epithelial lesions including squamous cell malignancies, they can also be detected in the normal oral mucosa in 10% to 20% of the adult population. however, the point of entry and the site of replication of hpv in the oral cavity are not known. since the gingival pocket is the only site in the oral mucosa where basal cells, known to be targets of hpv at other mucosal sites, are normally exposed to the environmen ... | 2005 | 15857068 |
| variant upstream regulatory region sequences differentially regulate human papillomavirus type 16 dna replication throughout the viral life cycle. | while the central role of the viral upstream regulatory region (urr) in the human papillomavirus (hpv) life cycle has been well established, its effects on viral replication factor expression and plasmid replication of hpv type 16 (hpv16) remain unclear. some nonprototypic variants of hpv16 contain altered urr sequences and are considered to increase the oncogenic risk of infections. to determine the relationship between viral replication and variant urrs, hybrid viral genomes were constructed w ... | 2005 | 15857977 |
| p16ink4a, cdc6, and mcm5: predictive biomarkers in cervical preinvasive neoplasia and cervical cancer. | to analyse and compare expression patterns of three potential biomarkers-p16(ink4a), cdc6, and mcm5-and evaluate their use as predictive biomarkers in squamous and glandular cervical preinvasive neoplasia. | 2005 | 15858126 |
| establishment and characterization of a human first-trimester extravillous trophoblast cell line (tev-1). | research into the biology of human trophoblast invasion has been hampered by a lack of in vitro models. the aim of this study was to establish and characterize a human extravillous trophoblast cell line from the first-trimester placenta. | 2005 | 15866109 |
| detection of human papillomavirus type 16 in bowen's disease of the web-space of the foot. | 2005 | 15868648 | |
| human papillomavirus infection and p16(ink4a) protein expression in vulvar intraepithelial neoplasia and invasive squamous cell carcinoma. | vulvar intraepithelial neoplasia (vin) is defined histopathologically by distinctive abnormalities of cellular maturation and differentiation. the purpose of this study was to investigate the functional properties of vin related to expression of p16(ink4a) protein as well as to detection of human papillomavirus (hpv) type 16 by real-time polymerase chain reaction (rt-pcr) analysis. | 2005 | 15870532 |
| optimal cutoff of the hybrid capture ii human papillomavirus test for self-collected vaginal, vulvar, and urine specimens in a colposcopy referral population. | to estimate the optimal relative light unit ratio, as a measure of viral load, of the hybrid capture ii human papillomavirus (hpv) test in self-collected specimens for detecting cervical intraepithelial neoplasia (cin). | 2004 | 15874834 |
| performance of direct visual inspection of the cervix with acetic acid and magnification in a previously screened population. | to assess the screening performance of direct visual inspection with acetic acid and x2 magnification (viam) in a previously screened population, as performed by experienced gynecologic nurses with minimal training in viam. | 2004 | 15874851 |
| beyond human papillomavirus: the cervix, exogenous secondary factors, and the development of cervical precancer and cancer. | human papillomavirus (hpv) is the necessary but probably not sufficient cause of cervical precancer and cancer. secondary exogenous and endogenous factors, hpv cofactors, may contribute to the probability of a cancer-associated (oncogenic) hpv infection progressing to cervical precancer and cancer. for these cofactors to influence the natural history of hpv infection, they must act on cervical tissue to promote viral persistence, progression to precancer or cancer given viral persistence, or bot ... | 2004 | 15874868 |
| false-negative hybrid capture ii results related to altered adhesion molecule distribution in women with atypical squamous cells pap smear results and tissue-based human papillomavirus-positive high-grade cervical intraepithelial neoplasia. | we investigated the detection of high-risk human papillomavirus dna with the hybrid capture ii test (hcii; digene corporation, beltsville, md) and the presence and pattern of distribution of adhesion molecules in biopsy-proven high-grade neoplastic lesions containing high-risk hpv-dna from women with asc cytology results. | 2004 | 15874874 |
| synthesis of gossypol atropisomers and derivatives and evaluation of their anti-proliferative and anti-oxidant activity. | gossypol 1, gossypolone 2, and a series of bis 3 and half schiff's bases 4 of gossypol were synthesised and tested for anti-proliferative and anti-oxidant activity. (-)-gossypol (-)-1 was the most potent inhibitor of the proliferation of the hpv-16 keratinocyte cell line (using an mtt viability assay) with a gi50 of 4.8 microm. the bis schiff's base of (-)-gossypol with l-tyrosine ethyl ester (-)-3b was the most potent inhibitor of iron/ascorbate dependent lipid peroxidation (using the thiobarbi ... | 2005 | 15878283 |
| immune responses induced by lower airway mucosal immunisation with a human papillomavirus type 16 virus-like particle vaccine. | cervical cancer results from cervical infection by human papillomaviruses (hpv), especially hpv16. previous studies have shown that intramuscular vaccination of women with an hpv16 virus-like particle (vlp) vaccine induced a strong igg response and protected against genital hpv16 infection. however, an alternative route of administration that avoids parenteral injection while inducing mucosal immunity might facilitate vaccine implementation in some settings, and partially overcome the substantia ... | 2005 | 15882523 |
| hpv and other risk factors of oral cavity/oropharyngeal cancer in the czech republic. | an association between high-risk human papillomavirus (hr hpv) infection and a risk of development of a subgroup of head and neck cancers has been proposed recently. the main risk factors of oral and oropharyngal cancer observed in our population are smoking and alcohol consumption. the incidence of oral/oropharyngeal tumours in the czech republic is relatively high and there are no data available about the prevalence of hpv dna presence in these tumours. | 2005 | 15888110 |
| presence of high-risk mucosal human papillomavirus genotypes in primary melanoma and in acquired dysplastic melanocytic naevi. | some studies have shown that cutaneous and mucosal melanoma biopsy specimens harbour human papillomavirus (hpv), suggesting that this virus may play a role in development and progression of the tumour. | 2005 | 15888145 |
| human papillomavirus-associated plantar epidermoid cyst related to epidermoid metaplasia of the eccrine duct epithelium: a combined histological, immunohistochemical, dna-dna in situ hybridization and three-dimensional reconstruction analysis. | we recently proposed that certain palmoplantar epidermoid cysts may be related to eccrine ducts and that human papillomavirus (hpv) 60 may play a role in their pathomechanism. however, the origin of palmoplantar epidermoid cysts is still controversial. | 2005 | 15888153 |
| socioeconomic status and the risk of cervical intraepithelial neoplasia grade 3 among oncogenic human papillomavirus dna-positive women with equivocal or mildly abnormal cytology. | low socioeconomic status (ses) is a reported risk factor for cervical carcinoma, but few studies have taken into account adequately the possibly confounding effects of oncogenic human papillomavirus (hpv) infection as well as access to screening and subsequent treatment. | 2005 | 15889450 |
| kinetic analysis of the interactions of human papillomavirus e6 oncoproteins with the ubiquitin ligase e6ap using surface plasmon resonance. | cervical cancers evolve from lesions generated by genital human papillomaviruses (hpv). "low-risk" genital hpvs cause benign proliferations whereas "high-risk" types have the potential to progress into cancer. high-risk hpv e6 oncoproteins interact with the ubiquitin ligase e6ap and target several cellular proteins, including p53 and proteins of the magi family, towards ubiquitin-mediated degradation. e6ap, like other e6 binding proteins such as e6bp, irf-3 and paxillin, interacts with e6 via a ... | 2005 | 15890204 |
| role of the e1--e4 protein in the differentiation-dependent life cycle of human papillomavirus type 31. | the most highly expressed protein in the productive life cycle of human papillomaviruses (hpvs) is e1--e4, but its function is not well understood. to investigate the role of e1--e4, we undertook a genetic analysis in the context of the complete hpv type 31 (hpv31) genome. a mutant hpv31 genome (e4m9) was constructed that contained a stop codon in the e4 open reading frame at amino acid 9 and was silent in the overlapping e2 coding sequence. wild-type and mutant genomes were transfected into nor ... | 2005 | 15890911 |
| papillomavirus capsid mutation to escape dendritic cell-dependent innate immunity in cervical cancer. | infection with oncogenic human papillomaviruses (hpvs), typified by hpv type 16 (hpv16), is a necessary cause of cervical cancer. prophylactic vaccination with hpv16 l1 virus-like particles (vlps) provides immunity. hpv16 vlps activate dendritic cells and a potent neutralizing immunoglobulin g (igg) response, yet many cervical cancer patients fail to generate detectable vlp-specific igg. therefore, we examined the role of the innate recognition of hpv16 l1 in vlp-induced immune responses and its ... | 2005 | 15890912 |
| diversifying selection in human papillomavirus type 16 lineages based on complete genome analyses. | human papillomavirus type 16 (hpv16) is the primary etiological agent of cervical cancer, the second most common cancer in women worldwide. complete genomes of 12 isolates representing the major lineages of hpv16 were cloned and sequenced from cervicovaginal cells. the sequence variations within the open reading frames (orfs) and noncoding regions were identified and compared with the hpv16r reference sequence. this whole-genome approach gives us unprecedented precision in detailing sequence-lev ... | 2005 | 15890941 |
| protection of beagle dogs from mucosal challenge with canine oral papillomavirus by immunization with recombinant adenoviruses expressing codon-optimized early genes. | replication-deficient adenoviral (rad5) vaccines containing codon-optimized e1, e2, e4, and e7 genes of canine oral papillomavirus (copv) were tested singly or in combination to determine which vaccines could protect against mucosal challenge with copv. in three studies, groups of 4-6 beagle dogs were immunized subcutaneously (s.c.) with 10(11) rad5 at 8-10 weeks and 4-6 weeks prior to challenge with infectious copv particles at multiple oral mucosal sites. control dogs were immunized with equiv ... | 2005 | 15892962 |
| characterization of dna vaccines encoding the domains of calreticulin for their ability to elicit tumor-specific immunity and antiangiogenesis. | antigen-specific cancer immunotherapy and antiangiogenesis are feasible strategies for cancer therapy because they can potentially treat systemic tumors at multiple sites in the body while discriminating between neoplastic and non-neoplastic cells. we have previously developed a dna vaccine encoding calreticulin (crt) linked to human papillomavirus-16 e7 and have found that this vaccine generates strong e7-specific antitumor immunity and antiangiogenic effects in vaccinated mice. in this study, ... | 2004 | 15893626 |
| human papillomavirus type and tobacco use as predictors of survival in early stage cervical carcinoma. | molecular and environmental co-factors are known risk factors for cervical cancer. the aim of this study was to define the prognostic significance of hpv 18 and its phylogenetically related viruses and smoking on survival in patients with early stage cervical cancer. | 2005 | 15894364 |
| psychosocial factors and the course of cervical intra-epithelial neoplasia: a prospective study. | to investigate the influence of psychosocial factors on the course of cervical intra-epithelial neoplasia (cin). | 2005 | 15894367 |
| modifiable risk factors associated with clearance of type-specific cervical human papillomavirus infections in a cohort of university students. | previous findings regarding risk factors for human papillomavirus (hpv) persistence, other than viral determinants, identified from prospective cohort studies have been inconsistent in part because study designs have differed with respect to differing hpv detection methods and varying lengths of follow-up time. therefore, the objectives of this study were to continue the search for epidemiologic risk factors of persistent cervical hpv infections and determine what behaviors differed between thos ... | 2005 | 15894665 |
| synchronous invasive squamous cell carcinoma and clear cell adenocarcinoma of the uterine cervix: a different human papillomavirus status. | a multiple primary invasive carcinoma of the cervix is a rare condition and is seldom composed of squamous cell carcinoma and clear cell adenocarcinoma. | 2005 | 15896829 |
| prevalence of types 16 and 33 is increased in high-risk human papillomavirus positive women with cervical intraepithelial neoplasia grade 2 or worse. | high-risk human papillomavirus (hrhpv) types are causally related to cervical cancer and its high-grade precursor lesions. the risk posed by the different hrhpv types for the development of cervical intraepithelial neoplasia grade 2 or worse (> or =cin2) needs to be established. here, we present the hrhpv type-distribution in relation to cytology and histology for women participating in a cervical screening program. from 44,102 women who participated in a population-based cervical screening prog ... | 2005 | 15900579 |
| human papillomavirus genotypes and the p53 codon 72 polymorphism in cervical cancer of northeastern thailand. | human papillomavirus (hpv) infection including sub-strain identification was studied in patients with squamous cell cervical cancer (scca) in northeastern thailand. subjects were 90 cases of scca and 100 healthy controls. prevalence of high-risk group of hpv infection in the controls and the scca patients were 13.0% and 86.7%, respectively. the hpv infection significantly increased the risk for cervical cancer 43.5-fold (95% confidential interval: 17.5-110.6; p <0.00001). among hpv carrier patie ... | 2005 | 15905603 |
| comparison between in situ hybridization and real-time pcr technique as a means of detecting the integrated form of human papillomavirus 16 in cervical neoplasia. | integration of the human papillomavirus (hpv) genome is thought to be one of the causes of cancer progression. however, there is controversy concerning the physical status of hpv 16 in premalignant cervical lesions, and there have been no reports on the concordance between detection of the integrated form of hpv16 by real-time pcr and by in situ hybridization. we investigated specimens of cervical intraepithelial neoplasia (cin) and invasive carcinomas for the physical status of hpv 16 by real-t ... | 2005 | 15905694 |
| methylation of human papillomavirus genomes in cells of anal epithelia of hiv-infected men. | intra-anal malignancies disproportionately affect individuals who engage in anal intercourse because of infection with human papillomaviruses (hpvs), with an increased risk attributed to infection with hiv because of a declining immunity against hpvs. long-term persistence of hpvs suggests yet other mechanisms that determine the clinical outcome, however. because methylation of hpv dna represses oncogene expression in cervical samples, we investigated whether this mechanism also occurs in hiv-po ... | 2005 | 15905729 |
| disruption of p53 by the viral oncoprotein hpv16-e6 does not deregulate chromosomal homologous recombination in a transcriptional interference-free assay system. | in addition to its well established role in the maintenance of genome integrity by regulating transcription of genes involved in cell cycle arrest and programmed cell death, the tumour suppressor p53 has also been shown to inhibit spontaneous chromosomal homologous recombination (hr) between adjacent transcription units, raising the possibility that p53 may prevent chromosomal rearrangements by suppressing hr between repetitive dna elements (ectopic hr). consistent with its role in the maintenan ... | 2005 | 15908792 |
| human papilloma virus and breast cancer. | 2005 | 15911436 | |
| the role of diet and nutrition in cervical carcinogenesis: a review of recent evidence. | our objective was to provide an update on recent epidemiologic evidence about the role of diet and nutrition on the risk of human papillomavirus (hpv) persistence and cervical neoplasia, taking hpv into account. we conducted a systematic review and qualitative classification of all observational studies controlling for hpv infection published between march 1995 and november 2003 and of all randomized clinical trials published between january 1991 and november 2003. scientific evidence was classi ... | 2005 | 15912536 |
| [immunomodulatory treatment with beta-interferon in patients with cervical intraepithelial neoplasia and human papillomavirus infection: long-term follow-up]. | the aim of this study was to evaluate the long-term efficacy of beta-interferon treatment in the management of cervical intraepithelial neoplasia associated with human papillomavirus (hpv) infection in a selected group of patients. thirty-nine patients who had histologically proven cervical intraepithelial neoplasia i or ii with concurrent hpv infection were administered 27,000,000 iu of intramuscular beta-interferon. periodical controls were carried out based on papanicolaou smears and/or colpo ... | 2005 | 15915229 |
| preliminary evaluation of a cervical self-sampling device with liquid-based cytology and multiparameter molecular testing. | to compare the collection of a liquid-based cell sample using a cervical self-sampling device and traditional sampling using cytologic and molecular-based tests. | 2005 | 15916209 |
| a tailored multi-media campaign to promote the human papillomavirus cohort study to young women. | human papillomavirus (hpv), a sexually transmitted disease, is now recognized as the major cause of cervical cancer. the cancer research center of hawaii developed a low-cost and culturally sensitive multimedia campaign to recruit oahu women to their hpv cohort study, specifically targeting women 18 to 35 years of age. | 2004 | 15916999 |
| enlarged squamous cell nuclei in cervical cytologic specimens from perimenopausal women ("pm cells") : a cause of asc overdiagnosis. | we studied the appropriateness of interpreting squamous cells with enlarged, smooth, bland nuclei in perimenopausal women ("pm cells") as atypical squamous cells (ascs). papanicolaou smears (paps) from 100 women (40-55 years old) with a cytologic interpretation of asc of undetermined significance (ascus) and human papillomavirus (hpv) testing or a biopsy within 6 months were reviewed by 2 observers without knowledge of the biopsy diagnosis or hpv results. cases in which both reviewers agreed tha ... | 2005 | 15923175 |
| sexual behaviour and high risk human papillomavirus infections in japanese women. | 2005 | 15923306 | |
| immunohistochemical demonstration of multiple hpv types in laryngeal squamous cell carcinoma. | the aim of this study was to determine the prevalence of human papillomaviruses (hpv) types 6, 11, 16, 18, 31, 33, 42, 51, 52, 56 and 58 in laryngeal squamous cell carcinoma specimens using immunohistochemical reactions and to correlate the presence of hpv with the clinical and pathological characteristics of these patients. tissue samples were collected from 40 patients with primary laryngeal squamous cell carcinoma (lscc) and from 33 subjects with non-neoplastic laryngeal lesions or laryngeal ... | 2005 | 15924277 |
| the human papillomavirus 16 e6 protein can either protect or further sensitize cells to tnf: effect of dose. | high-risk strains of human papillomavirus, including hpv 16, cause human cervical carcinomas, due in part to the activity of their e6 oncogene. e6 interacts with a number of cellular proteins involved in host-initiated apoptotic responses. paradoxically, literature reports show that e6 can both protect cells from and sensitize cells to tumor necrosis factor (tnf). to examine this apparent contradiction, e6 was transfected into u2os cells and stable clones were treated with tnf. intriguingly, clo ... | 2005 | 15933739 |
| human papillomavirus type 18 variant lineages in united states populations characterized by sequence analysis of lcr-e6, e2, and l1 regions. | while hpv 16 variant lineages have been well characterized, the knowledge about hpv 18 variants is limited. in this study, hpv 18 nucleotide variations in the e2 hinge region were characterized by sequence analysis in 47 control and 51 tumor specimens. fifty of these specimens were randomly selected for sequencing of an lcr-e6 segment and 20 samples representative of lcr-e6 and e2 sequence variants were examined across the l1 region. a total of 2770 nucleotides per hpv 18 variant genome were con ... | 2005 | 15936050 |
| outcome after a cervical cytology report of low-grade squamous abnormality in australia. | the current study was undertaken to assist with providing an evidence-based approach to the management of women with cervical cytology reports of low-grade squamous abnormality as used in the australian organized screening program. | 2005 | 15937925 |
| hpv 16 e6 sequence variations in indian patients with cervical neoplasia. | in a cross-sectional study performed between june 2001 and november 2003, the hpv 16 e6 gene of 50 women with cervical neoplasia and 20 cytologically normal women ('controls') was sequenced following amplification by pcr. the 350t to g variant was seen in 35 (70%) of 50 patients' isolates while it was seen in only 3 (15%) of the 20 isolates from the 'controls'. the higher occurrence of the 350g variant among the patients was statistically significant (p<0.01). isolates from patients were grouped ... | 2005 | 15939534 |
| [intraepithelial cervical squamous lesions: comparison between pap-test, colposcopy, phase contrast microscopy and histology; usefulness of their synergic use]. | in the last 10 years the tumors of cervix have showed a significant reduction in incidence, while the preneoplastic lesions are increased (linked often to human papilloma virus [hpv] infection), and so it is enhanced the role of early diagnosis. | 2005 | 15940075 |
| acquisition and persistence of human papillomavirus infection in younger men: a prospective follow-up study among danish soldiers. | no data is yet available on incidence or persistence of human papillomavirus (hpv) infection in men. we enrolled 374 younger male conscripts (18-29 years) in a prospective study, and they were examined twice with an interval of 6 to 8 months. data collection included a questionnaire and a sample of cells from the penis for hpv detection using pcr. in addition, the presence of chlamydia trachomatis dna was assessed in urine samples by means of pcr. the hpv prevalence at the first and second exami ... | 2005 | 15941967 |
| extended half-life upon binding of destabilized intrabodies allows specific detection of antigen in mammalian cells. | the ectopic expression of antibody fragments inside mammalian cells (intrabodies) is a challenging approach for probing and modulating target activities. we previously described the shuttling activity of intracellularly expressed escherichia coli beta-galactosidase conferred by the single-chain fv (scfv) fragment 13r4 equipped with nuclear import/export signals. here, by appending to scfvs the proteolytic pest signal sequence (a protein region rich in proline, glutamic acid, serine and threonine ... | 2005 | 15943819 |
| b lymphocyte activation by human papillomavirus-like particles directly induces ig class switch recombination via tlr4-myd88. | vaccination with human papillomavirus type 16 (hpv16) l1 virus-like particles (vlp) induces both high titer neutralizing igg and protective immunity. because protection from experimental infection by papillomavirus is mediated by neutralizing igg, we sought the mechanisms that trigger humoral immunity to hpv16 l1 vlp. we find that hpv16 l1 vlp bind to murine b lymphocytes thereby inducing activation-induced cytidine deaminase expression and ig class switch recombination to cause the generation o ... | 2005 | 15944297 |
| ultra-deformable liposomes containing bleomycin: in vitro stability and toxicity on human cutaneous keratinocyte cell lines. | formulations of ultra-deformable liposomes containing bleomycin (bleosome) have previously been described and proposed for topical treatment of skin cancer [lau, k.g., chopra, s., maitani, y., 2003. entrapment of bleomycin in ultra-deformable liposomes. s. t. p. pharm. sci. 13, 237-239]. in this study, the stability of various bleosome formulations was characterised and a purification process was established to isolate bleosome for testing on cultures of either human cutaneous keratinocytes (neb ... | 2005 | 15946810 |
| a single amino acid substitution improves the in vivo immunogenicity of the hpv16 oncoprotein e7(11-20) cytotoxic t lymphocyte epitope. | one of the few and most extensively studied human papilloma virus (hpv) type 16 oncoprotein e7-derived cytotoxic t lymphocyte (ctl) epitopes is ymldlqpett, presented to ctl by hla-a2.1. we previously identified an altered peptide ligand (apl) of this epitope with increased binding affinity for hla-a2.1, ymldlqpetv. herein, the in vivo immunogenicity of this apl was investigated in hla-a2.1 transgenic hhd mice. both in vitro and direct ex vivo analysis, performed using newly generated hhd tetrame ... | 2005 | 15963358 |
| diagnosis and management of oncogenic cervical human papillomavirus infection. | cervical hpv infection should be managed less as a typical sti and more as a strong risk factor predisposing to cervical cancer development. hpv infection is undeniably transmitted predominately through sexual contact.however, the fact that more than 80% of women followed over time will acquire at least one hr-hpv infection reflects the ubiquitous nature of the infection and the ease of transmission. although the behavioral profiles typically associated with an increased risk for sti (including ... | 2005 | 15963882 |
| multiple neoplastic lesions of the lower genital tract in a human papilloma virus-infected kidney-pancreas allograft recipient: a case report. | states of immunodeficiency are associated with an increased rate of certain cancers. immunosuppressed allograft recipients are at high risk of human papilloma virus (hpv)-related de novo malignancies. a female pancreas plus kidney transplant patient developed multiple genital malignancies within 6 years. the genome of human papilloma virus type 16 was detected in malignant lesions obtained from surgical procedures. all detected lesions were removed at an early state of development. | 2005 | 15964348 |
| [modulator role of p16ink4a in human papillomavirus infection]. | 2005 | 15970162 | |
| the impact of high risk human papillomavirus testing in an inner london colposcopy clinic. | this is an audit of a new technique to improve the colposcopy service. samples were tested for high risk hpv dna using digene hybrid capture ii. sixty-four percent of the sampled women under 30 had detectable high risk hpv dna, decreasing to 44% in 30--39 year olds and to 27% in women over 40. high risk hpv prevalence increased with severity of cytology, although 22% with normal colposcopy had detectable high risk hpv. of those women treated for cervical dysplasia, 83% had detectable high risk h ... | 2005 | 15977240 |
| technology evaluation: hspe7 (stressgen). | stressgen is developing hspe7, a recombinant fusion protein comprising the human papillomavirus (hpv) e7 antigen and the heat shock protein hsp65 from mycobacterium bovis, as a potential therapy for conditions associated with hpv infection. this therapy is currently undergoing phase iii clinical trials. | 2005 | 15977424 |
| financial requirements of immunisation programmes in developing countries: a 2004-2014 perspective. | vaccines are a key contributor to public health, especially in developing countries. despite numerous demonstrations of the cost-effectiveness of immunisation, vaccines spending accounted for only 1.7% of the total pharmaceutical market in 2002, when unicef estimated that 34 million children were not reached by routine immunisation, most of them in developing countries. several international organizations or initiatives, like the global alliance for vaccines and immunisation (gavi), have defined ... | 2005 | 15979769 |
| prostate cancer and sexually transmitted diseases: a meta-analysis. | prostate cancer is the most common neoplasm of american men and the second most common cause of cancer-related deaths. research suggests that infection and subsequent inflammation may be an important risk factor in the pathogenesis of prostate cancer. in this meta-analysis, we examine the current epidemiological evidence for the association between specific sexually transmitted diseases (stds) and prostate cancer. | 2005 | 15988645 |
| emerging therapies for human papillomavirus infection. | human papillomavirus (hpv) is the most common sexually transmitted infection, with > 50% of sexually active women being affected. the virus causes a wide variety of benign and pre-malignant epithelial tumours and although most infections are transient, it is estimated that 1% of the sexually active population in the us have clinically apparent genital warts. a subset of genital hpvs, termed high-risk hpvs, is highly associated with the development of genital cancers including cervical carcinoma. ... | 2001 | 15989521 |
| determinants of human papillomavirus-negative, low-grade squamous intraepithelial lesions in the atypical squamous cells of undetermined significance/low-grade squamous intraepithelial lesions triage study (alts). | although low-grade squamous intraepithelial lesions (lsil) most often are the result of infection by human papillomaviruses (hpv), a small proportion of women with lsil have negative hpv tests. using the atypical squamous cells of undetermined significance/lsil triage study (alts) population, the authors evaluated the significance of hpv-negative lsil. | 2005 | 15991244 |
| colposcopy vs hybrid capture ii assay in detection of cervical human papilloma virus infection. | considering the relationship between high-risk human papillomavirus types and the presence or subsequent development of cervical high-grade preinvasive lesions, the aim of the study was to determine if the hybrid capture ii test can be used to triage women with atypical colposcopic findings. | 2005 | 15991532 |
| imiquimod 5% cream (aldara). | imiquimod is a novel synthetic molecule with potent immune-modifying activities. formulated in a 5% vanishing cream as aldara, this self-applied therapy has shown good efficacy and safety in the treatment of external genital and perianal warts caused by human papillomavirus (hpv) infection (condyloma acuminata). the molecule does not demonstrate direct antiviral activity, but through induction of cytokines results in immune-based resolution of wart tissue and reduction of viral burden. phase iii ... | 1998 | 15991984 |
| involvement of nuclear export in human papillomavirus type 18 e6-mediated ubiquitination and degradation of p53. | the e6 protein from high-risk human papillomaviruses (hpvs) targets the p53 tumor suppressor for degradation by the proteasome pathway. this ability contributes to the oncogenic potential of these viruses. however, several aspects concerning the mechanism of e6-mediated p53 degradation at the cellular level remain to be clarified. this study therefore examined the role of cell localization and ubiquitination in the e6-mediated degradation of p53. as demonstrated within, following coexpression bo ... | 2005 | 15994771 |
| [the association of hela cell apoptosis induced by arsenic trioxide with the down-regulation of hpv18 e6 oncogene and inhibition of telomerase activity]. | to study the effect of as2o3 induction of hela cell apoptosis on hpv18 e6 expression and telomerase activity. | 2005 | 15996315 |