Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| evaluation of factors important in modeling plasma concentrations of tetracycline hydrochloride administered in water in swine. | to model the plasma tetracycline concentrations in swine (sus scrofa domestica) treated with medication administered in water and determine the factors that contribute to the most accurate predictions of measured plasma drug concentrations. | 2012 | 23013192 |
| from bench to bedside: initial experience with the primus drug-coated balloon catheter. | drug-coated balloon (dcb) technology has emerged as a promising therapy particularly in the treatment of coronary in-stent restenosis. although a variety of devices are available for clinical use, clinical outcomes have been variable and scope for significant improvement exists. | 2012 | 23018430 |
| cryptosporidium scrofarum n. sp. (apicomplexa: cryptosporidiidae) in domestic pigs (sus scrofa). | we describe the morphological, biological, and molecular characteristics of cryptosporidium pig genotype ii and propose the species name cryptosporidium scrofarum n. sp. to reflect its prevalence in adult pigs worldwide. oocysts of c. scrofarum are morphologically indistinguishable from c. parvum, measuring 4.81-5.96 μm (mean=5.16)×4.23-5.29 μm (mean=4.83) with a length to width ratio of 1.07±0.06 (n=400). oocysts of c. scrofarum obtained from a naturally infected pig were infectious for 8-week- ... | 2012 | 23021264 |
| adjuvants to prolong the local anesthetic effects of coated microneedle products. | the objective of this study was to identify an adjuvant for anesthetics coated on microneedles to provide rapid onset and prolonged analgesic action with minimal skin tissue reaction. aqueous lidocaine or prilocaine formulations with or without clonidine or the related analogs, guanfacine and apraclonidine, were dip-coated onto polymeric microneedles. the amount of lidocaine or prilocaine coated onto the microneedles was assessed by high performance liquid chromatography (hplc). delivery efficie ... | 2012 | 23022295 |
| evaluation of domestic and yucatan swine nasal sinus anatomy as models for future sinonasal research of medications delivered by standard instruments used in functional endoscopic sinus surgery. | there is a need to find an animal model to study new medications to improve mucosal wound healing after functional endoscopic sinus surgery (fess). current literature suggests swine as a potential candidate. the lack of information correlating swine computer tomography (ct) and endoscopic sinonasal anatomy prompted us to investigate them in the domestic and yucatan swine to determine their feasibility as models to test new medications and drug-embedded stents applied using fess techniques. | 2013 | 23038683 |
| edema and tetraparesis in a miniature pig after allogeneic hematopoietic cell transplantation. | a 3-mo-old, 12-kg, intact, miniature pig presented with severe neurologic signs on day 8 after hematopoietic cell transplantation. this pig had received an immunosuppressive regimen before transplantation that included an anticd3 immunotoxin for t-cell depletion, 100 cgy of total-body irradiation, and cyclosporine for 45 d. the pig began exhibiting erythematous lesions on posttransplantation day 7. he also demonstrated increased conscious proprioceptive deficits and recumbency but normal mentati ... | 0 | 23043783 |
| capsid protein evolution and comparative phylogeny of novel porcine parvoviruses. | in addition to the well known "classical" porcine parvovirus (ppv1; responsible for reproductive failure of susceptible sows) several new porcine parvoviruses have been recognized (ppv2, ppv3 and ppv4) in recent years. the genetic variation, characteristics and evolutionary factors shaping these novel ppvs were studied by comparing the complete capsid (cap) genes of ppvs from domestic pigs and wild boars. using bayesian coalescent methods we estimated the rate of nucleotide substitution for ppv2 ... | 2013 | 23044400 |
| mri quantification of left ventricular function in microinfarct versus large infarct in swine model. | to quantify, using mri, the acute impacts of defined volume and sizes of coronary microemboli on myocardial viability and left ventricular (lv) function and to use lad occlusion/reperfusion, as a reference. a total of 28 farm pigs were used in this study. eight animals were used as controls. successful coronary interventions were performed under x-ray fluoroscopy in 16 pigs to induce microinfarct (delivery of 16 mm(3) of 40-120 μm) and large infarct (90 min lad occlusion/reperfusion). on day 3, ... | 2013 | 23065097 |
| cathelicidins: family of antimicrobial peptides. a review. | cathelicidins are small, cationic, antimicrobial peptides found in humans and other species, including farm animals (cattle, horses, pigs, sheep, goats, chickens, rabbits and in some species of fish). these proteolytically activated peptides are part of the innate immune system of many vertebrates. these peptides show a broad spectrum of antimicrobial activity against bacteria, enveloped viruses and fungi. apart from exerting direct antimicrobial effects, cathelicidins can also trigger specific ... | 2012 | 23065264 |
| relationship between intrathoracic pressure and hemodynamics during cardiopulmonary resuscitation in a porcine model of prolonged cardiac arrest. | the influences of intrathoracic pressure (itp) to hemodynamic and respiratory parameters during cardiopulmonary resuscitation (cpr) are confusing. in this research, we investigated the phasic changes of itp during cpr and reveal the relationships among the hemodynamics, respiratory parameters, and itp. | 2012 | 23075711 |
| evaluation of efficacy and dose response of different paclitaxel-coated balloon formulations in a novel swine model of iliofemoral in-stent restenosis. | the authors aimed to validate a novel iliofemoral in-stent restenosis (isr) model for the efficacy evaluation of paclitaxel-coated balloons (pcb) using the familial hypercholesterolemic swine (fhs). | 2012 | 23078739 |
| endoscopic full-thickness biopsy of the gastric wall with defect closure by using an endoscopic suturing device: survival porcine study. | the pathogenesis of several common gastric motility diseases and functional gi disorders remains essentially unexplained. gastric wall biopsies that include the muscularis propria to evaluate the enteric nervous system, interstitial cells of cajal, and immune cells can provide important insights for our understanding of the etiology of these disorders. | 2012 | 23078926 |
| the effects of a newly developed miniaturized mechanical chest compressor on outcomes of cardiopulmonary resuscitation in a porcine model*. | when the duration of cardiac arrest is prolonged, reperfusion of the vital organs by effective chest compression is the most important intervention for successful resuscitation. we investigated the effects of a newly developed miniaturized chest compressor on the outcomes of cardiopulmonary resuscitation. | 2012 | 23080437 |
| detection and phylogenetic characterization of hepatitis e virus genotype 3 in a captive wild boar in thailand. | hepatitis e virus (hev) was studied in different types of wild boar captive settings in thailand, including a wildlife breeding research station, zoo, and commercial wild boar farm, which were located in different locations of thailand. fifty-one fecal samples were collected and screened for hev rna and then analyzed. one sample obtained from a wildlife breeding research station in ratchaburi province was hev positive. phylogenetic characterization revealed that the virus was hev genotype 3 and ... | 2012 | 23082533 |
| african swine fever virus infection in ornithodoros ticks. | african swine fever virus (asfv) is an arbovirus which is vectored by soft ticks of the ornithodoros spp. and in the sylvatic cycle infects wart hogs and bush pigs. asfv infection of domestic swine causes a high mortality disease. on the other hand, asfv infection of the tick can result in a high-titered and persistent infection depending upon the asfv isolate and the tick combination. recently, morphological, classical virology (titration) and recombinant asfv have been used to study the cellul ... | 2013 | 23085123 |
| diagnostics and epidemiology of alveolar echinococcosis in slaughtered pigs from large-scale husbandries in germany. | by means of the official meat inspection of domestic pigs, exceptionally high proportions of livers affected by encapsulated nodules containing whitish to light yellow, viscous to pasty material ("microabscesses") were detected. the swine had been raised on four different farms, being located in distinct regions of germany (brandenburg, thuringia, upper franconia). macroscopical and histological examination of 77 samples of livers revealed granulomatous to necrotizing hepatitis with attendance o ... | 2013 | 23093207 |
| [detecting selection signatures on x chromosome in pig through high density snps]. | in the process of domestic pig breeding, many important economic traits were subject to strong artificial se-lection pressure. with the availability of high density single nucleotide polymorphism (snp) markers in farm animals, selection occurring in those traits could be traced by detecting selection signatures on genome, and the genes experiencing selection can also be further mined based on selection signatures. due to the special characteristic of x chromosome, many approaches of genetic anal ... | 2012 | 23099781 |
| [manual annotation of the pig whole genomic sequence using otterlace software]. | in november 2009, scientists from the us, uk, and other countries announced the complete genome sequence draft of the domestic pig. with the release of improved versions of the pig genome assembly and the increase of correctly assembled sequenced fragments over the past two years, it is particularly urgent to have the pig genes annotated at whole-genome level. this article is aimed at introducing an excellent manual annotation tool, otterlace software, developed by sanger institute. we used cfl1 ... | 2012 | 23099791 |
| lactation biology symposium: lactocrine signaling and developmental programming. | lactocrine signaling is defined as transmission of bioactive factors from mother to offspring as a consequence of nursing. lactocrine transmission of signaling molecules may be an evolutionarily conserved process through which bioactive factors necessary for support of neonatal development are delivered postnatally. dependence on maternal resources for development in eutherian mammals extends into neonatal life for at least that period of time when nutrition is obtained solely from first milk (i ... | 2013 | 23100582 |
| vagal projections to the pylorus in the domestic pig (sus scrofa domestica). | the goal of the present study was to examine the precise localization of the brainstem motor and primary sensory (nodose ganglion) vagal perikarya supplying the pylorus in the domestic pig. using the fast blue retrograde tracing technique it has been established that all the vagal motor neurons projecting to the pylorus (about 337 ± 59 cells per animal) were localized bilaterally in the dorsal motor nucleus of the vagus nerve (dmx, 171 - left; 167 - right) and all other regions of the porcine br ... | 2012 | 23103024 |
| presence of torque teno sus virus in porcine circovirus type 2-associated disease in croatia. | torque teno virus (ttv) is ubiquitous and species-specific, and two different ttv species, torque teno sus virus 1 (ttsuv1) and torque teno sus virus 2 (ttsuv2), have been described in domestic pigs and wild boars. whether these two species are involved in clinical cases of porcine circovirus type 2-associated disease (pcvds) remains unknown. this study investigates the presence of ttsuv in 85 fetuses, pigs and wild boars infected by pcv2 and affected by pcvds other than postweaning multisystemi ... | 2012 | 23104589 |
| propolis modulates vitronectin, laminin, and heparan sulfate/heparin expression during experimental burn healing. | this study was aimed at assessing the dynamics of vitronectin (vn), laminin (ln), and heparan sulfate/heparin (hs/hp) content changes during experimental burn healing. | 2012 | 23125086 |
| biological heart rate reduction through genetic suppression of gα(s) protein in the sinoatrial node. | elevated heart rate represents an independent risk factor for cardiovascular outcome in patients with heart disease. in the sinoatrial node, rate increase is mediated by β(1) adrenoceptor mediated activation of the gα(s) pathway. we hypothesized that genetic inactivation of the stimulatory gα(s) protein in the sinoatrial node would provide sinus rate control and would prevent inappropriate heart rate acceleration during β-adrenergic activation. | 2012 | 23130123 |
| african swine fever virus serodiagnosis: a general review with a focus on the analyses of african serum samples. | african swine fever (asf) is an infectious disease that causes heavy mortality in domestic pigs. at present there is no vaccine against asf, and eradication in countries where the disease is endemic is based only on competent diagnosis programs and the sacrifice of infected animals. due to the presence of natural attenuated strains, certain infection conditions may result in reduced mortality. in these situations, the disease can be diagnosed by detection of specific antibodies. the use of class ... | 2013 | 23131491 |
| pathogenesis of african swine fever in domestic pigs and european wild boar. | african swine fever (asf) is among the most important viral diseases that can affect domestic and feral pigs. both clinical signs and pathomorphological changes vary considerably depending on strain virulence and host factors. acute infections with highly virulent virus strains lead to a clinical course that resembles a viral haemorrhagic fever that is characterized by pronounced depletion of lymphoid tissues, apoptosis of lymphocyte subsets, and impairment of haemostasis and immune functions. i ... | 2013 | 23137735 |
| review of the sylvatic cycle of african swine fever in sub-saharan africa and the indian ocean. | african swine fever (asf) is a major limiting factor for pig production in most of the countries in sub-saharan africa and the indian ocean. in the absence of vaccine, a good understanding of the ecology and epidemiology of the disease is fundamental to implement effective control measures. in selected countries of southern and east africa, the association between ornithodoros moubata ticks and warthogs has been described in detail in the literature. however, for many other countries in the regi ... | 2013 | 23142551 |
| african swine fever virus eradication in africa. | african swine fever was reported in domestic pigs in 26 african countries during the period 2009-2011. the virus exists in an ancient sylvatic cycle between warthogs (phacochoerus africanus) and argasid ticks of the ornithodoros moubata complex in many of the countries reporting outbreaks and in two further countries in the region. eradication of the virus from the countries in eastern and southern africa where the classic sylvatic cycle occurs is clearly not an option. however, the virus has be ... | 2013 | 23142552 |
| strong signatures of selection in the domestic pig genome. | domestication of wild boar (sus scrofa) and subsequent selection have resulted in dramatic phenotypic changes in domestic pigs for a number of traits, including behavior, body composition, reproduction, and coat color. here we have used whole-genome resequencing to reveal some of the loci that underlie phenotypic evolution in european domestic pigs. selective sweep analyses revealed strong signatures of selection at three loci harboring quantitative trait loci that explain a considerable part of ... | 2012 | 23151514 |
| analyses of pig genomes provide insight into porcine demography and evolution. | for 10,000 years pigs and humans have shared a close and complex relationship. from domestication to modern breeding practices, humans have shaped the genomes of domestic pigs. here we present the assembly and analysis of the genome sequence of a female domestic duroc pig (sus scrofa) and a comparison with the genomes of wild and domestic pigs from europe and asia. wild pigs emerged in south east asia and subsequently spread across eurasia. our results reveal a deep phylogenetic split between eu ... | 0 | 23151582 |
| [pharmacological influencing of abdominal aortic aneurysm model - animal experimental study, primary results]. | the aim of our work was to influence growth and histological changes in the wall of an experimentally induced aneurysm of the abdominal aorta in a large laboratory animal (domestic pig) by administering atorvastatin (3-hydroxy-3-methylglutaryl-coenzyme a reductase inhibitor). | 2012 | 23152990 |
| a gene expression atlas of the domestic pig. | this work describes the first genome-wide analysis of the transcriptional landscape of the pig. a new porcine affymetrix expression array was designed in order to provide comprehensive coverage of the known pig transcriptome. the new array was used to generate a genome-wide expression atlas of pig tissues derived from 62 tissue/cell types. these data were subjected to network correlation analysis and clustering. | 2012 | 23153189 |
| high-resolution autosomal radiation hybrid maps of the pig genome and their contribution to the genome sequence assembly. | the release of the porcine genome sequence offers great perspectives for pig genetics and genomics, and more generally will contribute to the understanding of mammalian genome biology and evolution. the process of producing a complete genome sequence of high quality, while facilitated by high-throughput sequencing technologies, remains a difficult task. the porcine genome was sequenced using a combination of a hierarchical shotgun strategy and data generated with whole genome shotgun. in additio ... | 2012 | 23153393 |
| african swine fever virus controls the host transcription and cellular machinery of protein synthesis. | throughout a viral infection, the infected cell reprograms the gene expression pattern in order to establish a satisfactory antiviral response. african swine fever virus (asfv), like other complex dna viruses, sets up a number of strategies to evade the host's defense systems, such as apoptosis, inflammation and immune responses. the capability of the virus to persist in its natural hosts and in domestic pigs, which recover from infection with less virulent isolates, suggests that the virus disp ... | 2013 | 23154157 |
| molecular hybrid positron emission tomography/computed tomography imaging of cardiac angiotensin ii type 1 receptors. | the goal of this study was to explore the feasibility of targeted imaging of the angiotensin ii type 1 receptor (at1r) in cardiac tissue, using clinical hybrid positron emission tomography/computed tomography (pet/ct). | 2012 | 23158533 |
| complete genome sequence of an h10n5 avian influenza virus isolated from pigs in central china. | an avian h10n5 influenza virus, a/swine/hubei/10/2008/h10n5, was isolated from pigs in the hubei province of central china. homology and phylogenetic analyses of all eight gene segments demonstrated that the strain was wholly of avian origin and closely homologous to the eurasian lineage avian influenza virus. to our knowledge, this is the first report of interspecies transmission of an avian h10n5 influenza virus to domestic pigs under natural conditions. | 2012 | 23166264 |
| toxocara cati larva migrans in domestic pigs--detected at slaughterhouse control in norway. | routine trichinella meat inspection at the slaughterhouse detected one larva in a pooled batch of 100 pig samples. the larva was sent to the norwegian veterinary institute (nvi) for species identification.morphological examination revealed that the larva was not trichinella spp. molecular analysis was performed. pcr and sequencing of 5s/its identified the larva as toxocara cati. a second round of digests was carried out at the meat inspection laboratory, in smaller batches to try to identify the ... | 2012 | 23171732 |
| reverse transcription loop-mediated isothermal amplification for rapid detection of japanese encephalitis virus in swine and mosquitoes. | japanese encephalitis (je) can infect many agriculturally important animals and humans, and has a high incidence in asia. one of the natural hosts of the mosquito-borne je virus (jev) is domestic pigs, which act as amplifier hosts. porcine infection results in fatal encephalitis, abortion, and stillbirth in pregnant sows, and hypospermia in boars. in this study, a rapid jev detection method for swine and mosquitoes was developed based on reverse transcription loop-mediated isothermal amplificati ... | 2012 | 23176446 |
| pig domestication and human-mediated dispersal in western eurasia revealed through ancient dna and geometric morphometrics. | zooarcheological evidence suggests that pigs were domesticated in southwest asia ~8,500 bc. they then spread across the middle and near east and westward into europe alongside early agriculturalists. european pigs were either domesticated independently or more likely appeared so as a result of admixture between introduced pigs and european wild boar. as a result, european wild boar mtdna lineages replaced near eastern/anatolian mtdna signatures in europe and subsequently replaced indigenous dome ... | 2013 | 23180578 |
| comparison of hepatitis e virus genotypes from rabbits and pigs in the same geographic area: no evidence of natural cross-species transmission between the two animals. | domesticated pigs have been shown to be a reservoir of genotypes 3 and 4 hepatitis e virus (hev). farmed rabbits were recently recognized as the host of a novel virus, rabbit hev. in order to determine whether hev is transmitted naturally between rabbits and pigs, a survey on hev infections was conducted in rabbits and pigs aged 2-4 months from rabbit and pig farms located near to each other in nine villages in three counties of hebei province, china. the overall anti-hev antibody positivity rat ... | 2013 | 23183309 |
| similar growth plate morphology in stapling and tension band plating hemiepiphysiodesis: a porcine experimental histomorphometric study. | the tension band plating technique for hemiepiphysiodesis is new and advocated because it is believed to reduce the risk of premature closure of the growth plate compared to stapling. the benefit of the tension band plating technique has not yet been proven in experimental or randomized clinical studies. ten weeks old domestic pigs in two randomized, paired studies were used. right proximal tibia was randomized to medial epiphysiodesis by either stapling or tension band plating. left side receiv ... | 2013 | 23192490 |
| carbon dioxide-contrasted computed tomography angiography: high pitch protocols and adapted injection parameters improve imaging quality. | to systematically investigate the impact of image acquisition and contrast injection parameters for co2-enhanced ct angiography (cta) of the aorto-iliac and peripheral arteries in a pig model using commercially available equipment. the aim was to establish an imaging protocol that is ready for use in human subjects. | 2013 | 23196833 |
| cellular immunity in asfv responses. | african swine fever virus (asfv) infection usually results in an acute haemorrhagic disease with a mortality rate approaching 100% in domestic pigs. however, pigs can survive infection with less-virulent isolates of asfv and may become chronically infected. surviving animals are resistant to challenge with homologous or, in some cases, closely related isolates of the virus indicating that pigs can develop protective immunity against asfv. during asymptomatic, non-virulent asfv infections natural ... | 2013 | 23201582 |
| indocyanine-green-loaded microballoons for biliary imaging in cholecystectomy. | we encapsulate indocyanine green (icg) in poly[(d,l-lactide-co-glycolide)-co-peg] diblock (plga-peg) microballoons for real-time fluorescence and hyperspectral imaging of biliary anatomy. icg-loaded microballoons show superior fluorescence characteristics and slower degradation in comparison with pure icg. the use of icg-loaded microballoons in biliary imaging is demonstrated in both biliary-simulating phantoms and an ex vivo tissue model. the biliary-simulating phantoms are prepared by embeddin ... | 2012 | 23214186 |
| pelvi-caliceal collecting system in swine--authors own anatomical classification. | experiments on humans and their organs produce legal, moral and ethical dilemmas. nowadays, the use of domestic swine as an experimental animal is increasing steadily. thus it is important to know the structure and characteristics of swine organs. | 2014 | 23214296 |
| comparison of african swine fever virus prevalence and risk in two contrasting pig-farming systems in south-west and central kenya. | we describe a horizontal survey of african swine fever virus (asfv) prevalence and risk factors associated with virus infection in domestic pigs in two contrasting production systems in kenya. a free range/tethering, low input production system in ndhiwa district of south-western kenya is compared with a medium input stall fed production system in kiambu district of central kenya. analysis of variance (anova) of data derived from cluster analysis showed that number of animals, number of breeding ... | 2013 | 23219357 |
| genetic characterisation of a porcine bocavirus detected in domestic pigs in uganda. | porcine bocaviruses (pobovs) are small linear ssdna viruses belonging to the genus bocavirus in the family parvoviridae. the genome encodes four proteins-the non-structural protein 1 (ns1), the np1 protein (unknown function) and the two structural proteins vp1 and vp2. in recent years, a number of different highly divergent pobov species have been discovered. pobovs have been shown to be present in pig populations in europe, asia and in the united states of america. in this study, we present the ... | 2013 | 23225112 |
| glycosylation facilitates transdermal transport of macromolecules. | stratum corneum, the outermost layer of skin, allows transport of only low-molecular weight (<500) lipophilic solutes. here, we report a surprising finding that avicins (avs), a family of naturally occurring glycosylated triterpenes with a molecular weight > 2,000, exhibit skin permeabilities comparable to those of small hydrophobic molecules, such as estradiol. systematic fragmentation of the av molecule shows that deletion of the outer monoterpene results in a 62% reduction in permeability, su ... | 2012 | 23236155 |
| three-dimensional regional strain analysis in porcine myocardial infarction: a 3t magnetic resonance tagging study. | previous studies of mechanical strain anomalies in myocardial infarction (mi) have been largely limited to analysis of one-dimensional (1d) and two-dimensional (2d) strain parameters. advances in cardiovascular magnetic resonance (cmr) methods now permit a complete three-dimensional (3d) interrogation of myocardial regional strain. the aim of this study was to investigate the incremental value of cmr-based 3d strain and to test the hypothesis that 3d strain is superior to 1d or 2d strain analysi ... | 2012 | 23237210 |
| application of change-point analysis to determine winter sleep patterns of the raccoon dog (nyctereutes procyonoides) from body temperature recordings and a multi-faceted dietary and behavioral study of wintering. | a multi-faceted approach was used to investigate the wintertime ecophysiology and behavioral patterns of the raccoon dog, nyctereutes procyonoides, a suitable model for winter sleep studies. by utilizing gps tracking, activity sensors, body temperature (tb) recordings, change-point analysis (cpa), home range, habitat and dietary analyses, as well as fatty acid signatures (fas), the impact of the species on wintertime food webs was assessed. the timing of passive bouts was determined with multipl ... | 2012 | 23237274 |
| effect of transgastric peritoneal access on peritoneal innate cellular immunity: experimental study in swine. | one of the main concerns of natural orifice surgery is the local and systemic impact on physiology. few studies have compared natural orifice transluminal endoscopic surgery (notes) with other surgical modalities. most studies are based on systemic variables such as postoperative serum cytokines, with conflicting results. surgical trauma induces an early inflammatory response, release of cytokines, and local leukocyte activation and oxidative burst. major surgical trauma is related to impairment ... | 2013 | 23239288 |
| domestication does not narrow mhc diversity in sus scrofa. | the major histocompatibility complex (mhc) is a multigene family of outstanding polymorphism. mhc molecules bind antigenic peptides in the peptide-binding region (pbr) that consists of five binding pockets (p). in this study, we compared the genetic diversity of domestic pigs to that of the modern representatives of their wild ancestors, the wild boar, in two mhc loci, the oligomorphic dqa and the polymorphic drb1. mhc nucleotide polymorphism was compared with the actual functional polymorphism ... | 2013 | 23239371 |
| identification of a novel host-specific igm protease in streptococcus suis. | streptococcus suis serotype 2 is a highly invasive, extracellular pathogen in pigs with the capacity to cause severe infections in humans. this study was initiated by the finding that igm degradation products are released after opsonization of s. suis. the objective of this work was to identify the bacterial factor responsible for igm degradation. the results of this study showed that a member of the ides family, designated ide(ssuis) (immunoglobulin m-degrading enzyme of s. suis), is responsibl ... | 2012 | 23243300 |
| [african swine fever in russian federation]. | african swine fever (asf) is an infectious viral disease that causes high economic losses due to the necessity of depopulation of pigs in affected areas, sanitary measures, trade restrictions, etc. the virus (asfv) is relatively stable in the unprocessed meat products and environment. thus, large areas are at risk due to free movement of people and products. the asfv does not affect people and animals, except the wild and domestic pigs. some ticks can become infected and carry the virus for year ... | 2016 | 23248852 |
| evaluation of a field-portable dna microarray platform and nucleic acid amplification strategies for the detection of arboviruses, arthropods, and bloodmeals. | highly multiplexed assays, such as microarrays, can benefit arbovirus surveillance by allowing researchers to screen for hundreds of targets at once. we evaluated amplification strategies and the practicality of a portable dna microarray platform to analyze virus-infected mosquitoes. the prototype microarray design used here targeted the non-structural protein 5, ribosomal rna, and cytochrome b genes for the detection of flaviviruses, mosquitoes, and bloodmeals, respectively. we identified 13 of ... | 2012 | 23249687 |
| porcine induced pluripotent stem cells require lif and maintain their developmental potential in early stage of embryos. | porcine induced pluripotent stem (pips) cell lines have been generated recently by using a cocktail of defined transcription factors, however, the features of authentic pips cells have not been agreed upon and most of published ips clones did not meet the stringent requirements of pluripotency. here, we report the generation of pips cells from fibroblasts using retrovirus carrying four mouse transcription factors (moct4, msox2, mklf4 and mc-myc, 4f). multiple lif-dependent pips cell lines were g ... | 2012 | 23251622 |
| hypothermia ameliorates gastrointestinal ischemic injury sustained in a porcine cardiac arrest model. | during cardiac arrest, the gastrointestinal tract is sensitive to ischemia. protection of the gastrointestinal tract is a critical factor in determining prognosis following cardiopulmonary resuscitation (cpr). this study seeks to determine the extent of gastrointestinal tract injury and the potential protective effect of inducing hypothermia following a porcine cardiac arrest model and cpr. | 2012 | 23253710 |
| orosomucoid expression profiles in liver, adipose tissues and serum of lean and obese domestic pigs, göttingen minipigs and ossabaw minipigs. | the acute phase protein orosomucoid (orm) has anti-inflammatory and immunomodulatory effects, and may play an important role in the maintenance of metabolic homeostasis in obesity-induced low-grade inflammation. even though the pig is a widely used model for obesity related metabolic symptoms, the expression of orm has not yet been characterized in such pig models. the objective of this study was to investigate the expression of orm1 mrna in liver, visceral adipose tissue, subcutaneous adipose t ... | 2013 | 23254161 |
| characterization of porcine ventral mesencephalic precursor cells following long-term propagation in 3d culture. | the potential use of predifferentiated neural precursor cells for treatment of a neurological disorder like parkinson's disease combines stem cell research with previous experimental and clinical transplantation of developing dopaminergic neurons. one current obstacle is, however, the lack of ability to generate dopaminergic neurons after long-term in vitro propagation of the cells. the domestic pig is considered a useful nonprimate large animal model in neuroscience, because of a better resembl ... | 2012 | 23258982 |
| teschoviruses and sapeloviruses in faecal samples from wild boar in spain. | teschovirus and sapelovirus are two genera of the picornaviridae family, comprising highly variable and heterogeneous enteric viruses, commonly found in faecal samples from domestic pigs. although both of them are also known to infect wild boar, studies on their presence in these wild suids are scarce. the present study aimed at determining the presence of porcine teschovirus (ptv) and sapelovirus (psv) in free-living wild boar populations, as well as to study their relationships with similar vi ... | 2012 | 23265238 |
| modulation of chemokine and chemokine receptor expression following infection of porcine macrophages with african swine fever virus. | african swine fever virus (asfv) is the only member of the asfarviridae, a large dna virus family which replicates predominantly in the cytoplasm. most isolates cause a fatal haemorrhagic disease in domestic pigs, although some low virulence isolates cause little or no mortality. the modulation of chemokine responses following infection of porcine macrophages with low and high virulence isolates was studied to indicate how this may be involved in the induction of pathogenesis and of effective im ... | 2012 | 23265239 |
| identification of genome-wide copy number variations among diverse pig breeds by array cgh. | recent studies have shown that copy number variation (cnv) in mammalian genomes contributes to phenotypic diversity, including health and disease status. in domestic pigs, cnv has been catalogued by several reports, but the extent of cnv and the phenotypic effects are far from clear. the goal of this study was to identify cnv regions (cnvrs) in pigs based on array comparative genome hybridization (acgh). | 2012 | 23265576 |
| nano-crystalline diamond-coated titanium dental implants - a histomorphometric study in adult domestic pigs. | promising biomaterial characteristics of diamond-coatings in biomedicine have been described in the literature. however, there is a lack of knowledge about implant osseointegration of this surface modification compared to the currently used sandblasted acid-etched ti-al6-v4 implants. the aim of this study was to investigate the osseointegration of microwave plasma-chemical-vapour deposition (mwp-cvd) diamond-coated ti-al6-v4 dental implants after healing periods of 2 and 5 months. twenty-four mw ... | 2013 | 23266005 |
| african swine fever in the north caucasus region and the russian federation in years 2007-2012. | since the introduction of the virus into the republic of georgia in 2007 african swine fever (asf) has become a large-scale epidemic involving the domestic pig population but wild boars are involved as well. from 2008 to 2009 the asf epidemic affected wild and domestic pigs in all the southern regions of the russian federation (rf). the driving force of the epidemic in its initial stages was direct contact between infected wild boars and between wild boars and traditionally free-ranging domestic ... | 2012 | 23266725 |
| effect of rescue breathing during cardiopulmonary resuscitation on lung function after restoration of spontaneous circulation in a porcine model of prolonged cardiac arrest. | the destruction of the pulmonary structure after cardiopulmonary resuscitation may lead to lung function breakdown. the aim of this study was to investigate lung function after cardiopulmonary resuscitation and the influence of rescue breathing on lung function. | 2013 | 23269128 |
| a survey of porcine reproductive and respiratory syndrome among wild boar populations in korea. | no information is currently available on porcine reproductive and respiratory syndrome virus (prrsv) infection in wild boars (sus scrofa) in korea. in this study, the status of prrs in wild boars was investigated. blood samples were collected from 267 wild boars from eight provinces in korea. four of the samples tested (1.5%) were positive for prrsv antibodies and eight (3.0%) were positive for antigens. of the virus-positive samples, three and five samples were typed as containing european (eu, ... | 2012 | 23271179 |
| mitochondrial dna evidence indicates the local origin of domestic pigs in the upstream region of the yangtze river. | previous studies have indicated two main domestic pig dispersal routes in east asia: one is from the mekong region, through the upstream region of the yangtze river (uryz) to the middle and upstream regions of the yellow river, the other is from the middle and downstream regions of the yangtze river to the downstream region of the yellow river, and then to northeast china. the uryz was regarded as a passageway of the former dispersal route; however, this assumption remains to be further investig ... | 2012 | 23272130 |
| african swine fever in the russian federation: spatio-temporal analysis and epidemiological overview. | african swine fever is viral disease of domestic and wild pigs which leads to almost total mortality and causes great economic losses due to absence of vaccine. having been introduced into the russian federation in 2007 the disease has spread widely in the southern region of the country and since 2011 has demonstrated a tendency to form a secondary endemic zone in the central part of the country. in the present study spatio-temporal patterns of asf diffusion in the populations of wild and domest ... | 2012 | 23274108 |
| transdermal delivery of adipocyte-derived stem cells using a fractional ablative laser. | chronic wound healing problems can pose a significant clinical challenge. transdermal delivery of adipose-derived stem cells (adsc) may be a possible solution to healing these recalcitrant, debilitating wounds. pretreatment of the skin with a fractionated laser has already been shown to assist transdermal drug delivery both in vitro and in vivo and may be an ideal approach to facilitating delivery of adsc to the target tissue. | 2013 | 23277622 |
| sem and stereomicroscopic analysis of cut marks in fresh and burned bone. | this study documents the prevalence of cut mark characteristics in fresh and burned domestic pig ribs. stab wounds from single edge serrated and smooth-edged knives were inflicted in the vertebral and sternal regions of each fresh rib. each rack of ribs was then divided into vertebral and sternal units. vertebral units were defleshed and their associated cut marks were examined using a stereomicroscope. sternal units were burned in an outdoor fire pit and examined with the addition of a scanning ... | 2013 | 23278902 |
| a new approach for rapidly assessing the risk of aujeszky's disease reintroduction into a disease-free spanish territory by analysing the movement of live pigs and potential contacts with wild boar. | aujeszky's disease (ad) causes significant economic losses in the spanish pig sector due to import trade restrictions imposed by disease-free countries. most regions of spain have achieved 'low ad prevalence' status as a result of an intensive national ad eradication programme involving vaccination and other measures. however, to achieve ad-free status that would eliminate trade restrictions, vaccination must be stopped. for this final stage of eradication, up to date and reliable estimates of t ... | 2014 | 23279743 |
| best practices for performing experimental surgery in swine. | the authors have extensive experience in surgery, anesthesia, and perioperative care in both miniature and domestic swine in the research setting. this manuscript describes the techniques that have been developed over the last four decades while developing porcine surgical models and their use in translational research. swine are conditioned and socialized during a seven-day period prior to entering into a survival surgical protocol. housing is designed to be nonstressful and accommodate the nee ... | 2013 | 23281597 |
| the impact of early goal-directed fluid management on survival in an experimental model of severe acute pancreatitis. | severe acute pancreatitis (sap) remains a life-threatening disease with classic etiology of systemic inflammatory response and mortality between 30 and 50 %. the aim of the present study is to compare two different treatment strategies of goal-directed hemodynamic management and evaluate their impact on survival, microcirculation, tissue oxygenation, and histopathologic damage in acute pancreatitis in a prospective animal study. | 2013 | 23287870 |
| apoptotic transcriptional profile remains activated in late remodeled left ventricle after myocardial infarction in swine infarcted hearts with preserved ejection fraction. | apoptosis is involved in both acute and chronic loss of cardiomyocytes after myocardial infarction (mi). to date, the pathophysiological significance of an apoptotic transcriptional profile activated in the post-ischemic remodeled myocardium, in the absence of hemodynamic factors secondary to left ventricular (lv) dysfunction, still remains to be determined. the mrna expression of pro- and anti-apoptotic factors was determined in a swine model of non-reperfused mi with preserved lv ejection frac ... | 2013 | 23295943 |
| high frequency of human blood in triatoma dimidiata captured inside dwellings in a rural community in the yucatan peninsula, mexico, but low antibody seroprevalence and electrocardiographic findings compatible with chagas disease in humans. | we studied a small rural community of 411 inhabitants localized in the state of campeche in the yucatan peninsula, mexico. in 44 collected triatomines captured inside the houses, human feeding source was revealed in 23 of 44 (52%) samples, and chicken feeding source was revealed in 16 of 44 (36%) samples. in a set of 29 triatomines, mouse was the feeding source in 13 (44%) samples, and dog was the feeding source in 7 (24%) samples. infection index with trypanosoma cruzi in collected triatomines ... | 2013 | 23296447 |
| septic shock non-thyroidal illness syndrome causes hypothyroidism and conditions for reduced sensitivity to thyroid hormone. | non-thyroidal illness syndrome (ntis) is part of the neuroendocrine response to stress, but the significance of this syndrome remains uncertain. the aim of this study was to investigate the effect of lipopolysaccharide (lps)-induced ntis on thyroid hormone (th) levels and th molecular targets, as well as the relationship between septic shock nuclear factor kappa-light-chain-enhancer of activated b cells (nf-kb) activation and th receptor β (thrb) gene expression at a multi-tissue level in a pig ... | 2013 | 23296981 |
| [model of genetic control of the number and location of nipples in domestic pig]. | in domestic pig, the number and location of nipples is considered a single quantitative trait, which has a complex spatial structure that includes bilaterality and a linear sequence of repetitive elements. according to this hypothesis, six inadherent biallelic loci are sufficient for a variety of traits observed in domestic pig populations. verification of representative samplings from five domestic pig populations of different origin (about 20 000 individuals) confirmed the theses of the hypoth ... | 2012 | 23297487 |
| food preferences of winter bird communities in different forest types. | food availability for forest birds is a function of habitat type, forest management regime, and season. in winter, it is also impacted by variations in the weather. in the current study we assessed the food preferences of wild bird populations in two types of forest (spruce and beech) during the months of november 2010 to april 2011 in the schwäbische alb biodiversity exploratory, south-western germany. our aim was to investigate whether local bird communities preferred fat-rich, carbohydrate-ri ... | 2012 | 23300878 |
| taenia solium taeniosis/cysticercosis in africa: risk factors, epidemiology and prospects for control using vaccination. | poor sanitary conditions, free-roaming of domestic pigs and lack of awareness of the disease play an important role in the perpetuation of the taenia solium taeniosis and cysticercosis in africa. traditional pig production systems known as the source of t. solium taeniosis/cysticercosis complex are predominant in the continent, representing 60-90% of pig production in rural areas. it has been reported that t. solium cysticercosis is the main cause of acquired epilepsy in human population and res ... | 2013 | 23312868 |
| a comparison of etanercept vs. infliximab for the treatment of post-arrest myocardial dysfunction in a swine model of ventricular fibrillation. | to compare the effects of two tnf-α antagonists, etanercept and infliximab, on post-cardiac arrest hemodynamics and global left ventricular function (lv) in a swine model following ventricular fibrillation (vf). | 2013 | 23313857 |
| regions of xy homology in the pig x chromosome and the boundary of the pseudoautosomal region. | sex chromosomes are subject to evolutionary pressures distinct from the remainder of the genome, shaping their structure and sequence content. we are interested in the sex chromosomes of domestic pigs (sus scrofa), how their structure and gene content compares and contrasts with other mammalian species, and the role of sex-linked genes in fertility. this requires an understanding of the xy-homologous sequence on these chromosomes.to this end, we performed microarray-based comparative genomic hyb ... | 2013 | 23320497 |
| characterization of nuclear localization signal in the n terminus of integrin-linked kinase-associated phosphatase (ilkap) and its essential role in the down-regulation of rsk2 protein signaling. | integrin-linked kinase-associated phosphatase (ilkap) is a serine/threonine (s/t) phosphatase that belongs to the protein phosphatase 2c (pp2c) family. many previous studies have demonstrated that ilkap plays key roles in the regulation of cell survival and apoptosis. researchers have thus far considered ilkap a cytoplasmic protein that negatively regulates integrin signaling by interacting with and phosphorylating integrin-linked kinase 1 (ilk1). in this study, we found that both endogenous and ... | 2013 | 23329845 |
| genomic characterization of pseudorabies virus strains isolated in italy. | in this study, we undertook the genomic characterization of 54 pseudorabies virus (prv) strains isolated in italy during 1984-2010. the characterization was based on partial sequencing of the ul44 (gc) and us8 (ge) genes; 44 strains (38 for gene ge and 36 for gc) were isolated on pig farms; 9 originated from dogs and 1 from cattle. these porcine prv strains, which were closely related to those isolated in europe and america in the last 20 years, and the bovine strain bovine/it/2441/1992 belong t ... | 2014 | 23331342 |
| research resource: the endometrium database resource (edr). | in order to understand the biology of the endometrium and potentially develop new diagnostic tools and treatments for endometrial diseases, the highly orchestrated gene expression/regulation that occurs within the uterus must first be understood. even though a wealth of information on endometrial gene expression/regulation is available, this information is scattered across several different resources in formats that can be difficult for the average bench scientist to query, integrate, and utiliz ... | 2013 | 23340253 |
| high-density dependence but low impact on selected reproduction parameters of brucella suis biovar 2 in wild boar hunting estates from south-western spain. | porcine brucellosis is a disease caused by brucella suis, which is characterized by reproductive disorders in pigs. the number of cases of swine brucellosis has risen in many european countries, likely because of the presence of a wild reservoir of b. suis in wild boar. this study aimed at evaluating factors that may influence the probability of infection with brucella spp. in wild boar and at assessing the impact of a previous contact with brucella spp. on reproductive parameters of wild boar. ... | 2014 | 23347330 |
| fluid resuscitation guided by sublingual partial pressure of carbon dioxide during hemorrhagic shock in a porcine model. | to avoid aggressive fluid resuscitation during hemorrhagic shock, fluid resuscitation is best guided by a specific measurement of tissue perfusion. we investigated whether fluid resuscitation guided by sublingual pco2 would reduce the amount of resuscitation fluid without compromising the outcomes of hemorrhagic shock. ten male domestic pigs weighing between 34 and 37 kg were used. forty-five percent of estimated blood volume was removed during an interval of 1 h. the animals were then randomize ... | 2013 | 23364438 |
| computed tomography-based diagnosis of diffuse compensatory enlargement of coronary arteries using scaling power laws. | glagov's positive remodelling in the early stages of coronary atherosclerosis often results in plaque rupture and acute events. because positive remodelling is generally diffused along the epicardial coronary arterial tree, it is difficult to diagnose non-invasively. hence, the objective of the study is to assess the use of scaling power law for the diagnosis of positive remodelling of coronary arteries based on computed tomography (ct) images. epicardial coronary arterial trees were reconstruct ... | 2013 | 23365197 |
| hysteresis in di independent mechanisms in threshold for transition between 1:1 and 2:2 rhythms in pigs. | previous studies have shown hysteresis in transition from 1:1 to 2:2 rhythms in action potential durations (apd) during decreasing and increasing cycle length pacing. as cycle lengths were constant, when alternans of apd occurred, so did of preceding diastolic intervals (dis), which engaged the restitution mechanism, i.e. dependence of apd on preceding di, making it impossible to determine whether the hysteresis originates from the di independent or dependent mechanism. by using pacing with expl ... | 2012 | 23365980 |
| molecular detection of hepatitis e virus in german domestic pigs. | in industrial countries, hepatitis e virus (hev) transmission to humans is predominantly assumed to be a zoonotic infection. recently, it has been demonstrated that about 50% of domestic pigs in germany carry hev-specific antibodies. however, further investigations concerning the distribution of hev in different age groups of german domestic pigs, phylogenetic analyses and the viral load in the porcine liver are still pending. liver samples of all age groups from herds in a pig-dense region in n ... | 2013 | 23367665 |
| ischemic post-conditioning and vasodilator therapy during standard cardiopulmonary resuscitation to reduce cardiac and brain injury after prolonged untreated ventricular fibrillation. | we investigated the effects of ischemic postconditioning (ipc) with and without cardioprotective vasodilatory therapy (cvt) at the initiation of cardiopulmonary resuscitation (cpr) on cardio-cerebral function and 48-h survival. | 2013 | 23376583 |
| size-related changes in foot impact mechanics in hoofed mammals. | foot-ground impact is mechanically challenging for all animals, but how do large animals mitigate increased mass during foot impact? we hypothesized that impact force amplitude scales according to isometry in animals of increasing size through allometric scaling of related impact parameters. to test this, we measured limb kinetics and kinematics in 11 species of hoofed mammals ranging from 18-3157 kg body mass. we found impact force amplitude to be maintained proportional to size in hoofed mamma ... | 2013 | 23382967 |
| determination of organ-specific anemia tolerance. | utilization of anemia tolerance reduces the need for and risks of perioperative transfusion. recent publications indicate that the critical limit for oxygen supply might not be the same for each organ system. therefore, we investigated the effects of acute dilutional anemia on heart, brain, kidneys, liver, small intestine, and skeletal muscle to quantify organ-specific tolerance of different levels of acute anemic hypoxia. we hypothesized that, in some organs, tissue hypoxia occurs before the cr ... | 2013 | 23385097 |
| early detection of acute mesenteric ischemia using diffusion-weighted 3.0-t magnetic resonance imaging in a porcine model. | the aim of this study was to investigate if 3.0-t diffusion-weighted magnetic resonance imaging (mri) can be used for early detection of acute occlusive and nonocclusive mesenteric ischemia. | 2013 | 23385397 |
| molecular characterization of japanese encephalitis virus strains prevalent in chinese swine herds. | japanese encephalitis virus (jev) is the leading cause of viral encephalitis in asia and domestic pigs serve as the amplifying hosts. in the present study, the full genomic sequences of two jev strains (hen0701 and sh0601) isolated from pigs in china were determined and compared with other 12 jev strains deposited in genbank. these two strains had an 88.8% nucleotide sequence similarity and 97.9% deduced amino acid sequence homology. hen0701 had high nucleotide sequence and high amino acid seque ... | 2013 | 23388434 |
| species-specific vesicular monoamine transporter 2 (vmat2) expression in mammalian pancreatic beta cells: implications for optimising radioligand-based human beta cell mass (bcm) imaging in animal models. | imaging of beta cell mass (bcm) is a major challenge in diabetes research. the vesicular monoamine transporter 2 (vmat2) is abundantly expressed in human beta cells. radiolabelled analogues of tetrabenazine (tbz; a low-molecular-weight, cell-permeant vmat2-selective ligand) have been employed for pancreatic islet imaging in humans. since reports on tbz-based vmat2 imaging in rodent pancreas have been fraught with confusion, we compared vmat2 gene expression patterns in the mouse, rat, pig and hu ... | 2013 | 23404442 |
| the foetal pig pineal gland is richly innervated by nerve fibres containing catecholamine-synthesizing enzymes, neuropeptide y (npy) and c-terminal flanking peptide of npy, but it does not secrete melatonin. | innervation of the mammalian pineal gland during prenatal development is poorly recognized. therefore, immunofluorescence studies of the pineals of 70- and 90-day-old foetuses of the domestic pig were performed using antibodies against tyrosine hydroxylase (th), dopamine-β-hydroxylase (dβh), neuropeptide y (npy) and c-terminal flanking peptide of npy (cpon). the investigated glands were supplied by numerous nerve fibres containing th and dβh. the density of these fibres was higher in the distal ... | 2013 | 23408385 |
| carbonic anhydrase-related protein xi: structure of the gene in the greater false vampire bat (megaderma lyra) compared with human and domestic pig. | carbonic anhydrase-related protein xi (ca-rp xi) is a member of the α-carbonic anhydrase family (encoded by the gene ca-11), which has lost features of the active site required for enzymatic activity. using pcr, we amplified ca-11 from genomic dna of the bat megaderma lyra. to elucidate the gene structure, we sequenced pcr products and compared their sequences with genomic and mrna sequences known from human and domestic pig. we identified and sequenced eight introns in the bat ca-11. five intro ... | 2013 | 23417223 |
| hepatic and splenic stiffness augmentation assessed with mr elastography in an in vivo porcine portal hypertension model. | to investigate the influence of portal pressure on the shear stiffness of the liver and spleen in a well-controlled in vivo porcine model with magnetic resonance elastography (mre). a significant correlation between portal pressure and tissue stiffness could be used to noninvasively assess increased portal venous pressure (portal hypertension), which is a frequent clinical condition caused by cirrhosis of the liver and is responsible for the development of many lethal complications. | 2013 | 23418135 |
| tamoxifen decreases the myofibroblast count in the healing bile duct tissue of pigs. | the aim of this study was to evaluate the effect of oral tamoxifen treatment on the number of myofibroblasts present during the healing process after experimental bile duct injury. | 0 | 23420165 |
| shen-fu injection reduces impaired myocardial β-adrenergic receptor signaling after cardiopulmonary resuscitation. | post-resuscitation myocardial dysfunction has been implicated as a major cause of fatal outcome in patients who survive initially successful cardiopulmonary resuscitation (cpr). in our previous study, we found that impaired myocardial β-adrenergic receptor (ar) signaling is a key mechanism in post-resuscitation myocardial dysfunction and shen-fu injection (sfi) can attenuate post-resuscitation myocardial dysfunction. however, whether sfi can prevent impaired post-resuscitation myocardial β-ar si ... | 2013 | 23422192 |
| survey of trichinella infections in domestic pigs from northern and eastern henan, china. | the aim of this work was to investigate the current situation of trichinella infections in swine in the cities of anyang and shanqiu in the henan province historically designated as trichinellosis-free. a total of 475 diaphragm muscle samples were collected from 2010 to 2011 and examined by trichinelloscopy and artificial digestion. no trichinella larvae were detected by trichinelloscopy; however, using the digestion method, 3.79% (18/475) of domestic pigs were deemed positive for trichinella. a ... | 2013 | 23422779 |