Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| sjhsp70, a recombinant schistosoma japonicum heat shock protein 70, is immunostimulatory and induces protective immunity against cercarial challenge in mice. | high levels of protective immunity can be induced in different animals immunized with radiation-attenuated (ra) schistosoma cercariae or schistosomula. however, the schistosome-derived molecules responsible for the strong protective effect elicited by ra schistosome larvae have not been identified or characterized. the 70-kda heat shock proteins of schistosomes are considered major immunogens, and may play an important role in stimulating high levels of innate and adaptive immune responses in an ... | 2015 | 26091761 |
| [effect of calcium cyanamid synthetic drug on schistosoma japonicum egg morphology]. | to study the morphological change of schistosoma japonicum eggs processed by calcium cyanamide synthetic drug, so as to provide the basis for further study of the mechanism that calcium cyanamide synthetic drug to schistosome eggs. | 2015 | 26094416 |
| [experimental study on determination of viability of schistosoma japonicum cercariae by staining]. | to determine the viability of schistosoma japonicum cercariae by staining. | 2015 | 26094419 |
| [research progress of taxonomy and discrimination techniques of oncomelania hupensis]. | oncomelania hupensis, which plays an important role in the transmission of schistosomiasis japonica, is the only intermediate host of schistosoma japonicum, and carrying out studies on its subspecies differentiation and genetic variation will have an important significance for schistosomiasis control. in this paper, the research progress of taxonomy and discrimination techniques of o. hupensis is reviewed at four levels, namely the morphological level, the cellular level, the protein level, and ... | 2015 | 26094433 |
| cloning, expression, and partial characterization of fbpa from schistosoma japonicum, a molecule on that the fluke may develop nutrition competition and immune evasion from human. | carbohydrate metabolism is the most important physiological process for schistosoma japonicum which resides in host. however, as a key glycolytic enzyme in carbohydrate metabolism, fructose-1,6-bisphosphate aldolase (fbpa), there is no study on its enzymatic kinetics and antigenic peptides. here, we report the gene cloning, expression, purification, and kinetics of the fbpa from s. japonicum (sjfbpa). after cloning, sjfbpa gene was introduced into pet-28a and transformed bl21, and a soluble his6 ... | 2015 | 26099237 |
| [expression profiling and immunofluorescence localization of the major egg antigen p40 of schistosoma japonicum in the liver of infected new zealand white rabbits]. | to examine the expression profile and immunofluorescence localization of the major egg antigen p40 of schistosoma japonicum (sjp40) during granuloma formation in the liver of infected new zealand white rabbits. | 2015 | 26111679 |
| three gorges dam: impact of water level changes on the density of schistosome-transmitting snail oncomelania hupensis in dongting lake area, china. | schistosomiasis remains an important public health issue in china and worldwide. oncomelania hupensis is the unique intermediate host of schistosoma japonicum, and its change influences the distribution of s. japonica. the three gorges dam (tgd) has substantially changed the ecology and environment in the dongting lake region. this study investigated the impact of water level and elevation on the survival and habitat of the snails. | 2015 | 26114956 |
| using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of schistosoma japonicum. | antibodies isolated from the local draining inguinal lymph node of field exposed-water buffaloes following challenge with schistosoma japonicum cercariae showed high reactivity towards s. japonicum antigen preparations and bound specifically to formaldehyde-fixed s. japonicum schistosomules. using this specific local immune response we produced a series of single-chain antibody fv domain libraries from the same lymph nodes. removal of phage that cross reacted with epitopes on adult parasites yie ... | 2015 | 26116907 |
| meta-analysis of the diagnostic efficiency of the questionnaires screening for schistosomiasis. | schistosomiasis is the second widespread tropical disease that affects the health of over 240 million people of 78 countries. questionnaires have been commonly used to diagnose schistosomiasis, while no meta-analysis of their efficacy had been reported previously. this meta-analysis was conducted to assess their diagnostic accuracy of schistosomiasis. studies published prior to december 1, 2014, that had used questionnaires as a diagnostic tool were searched in pubmed, medline, embase, and china ... | 2015 | 26122990 |
| the differential expression of immune genes between water buffalo and yellow cattle determines species-specific susceptibility to schistosoma japonicum infection. | water buffalo are less susceptible to schistosoma japonicum infection than yellow cattle. the factors that affect such differences in susceptibility remain unknown. a bos taurus genome-wide gene chip was used to analyze gene expression profiles in the peripheral blood of water buffalo and yellow cattle pre- and post-infection with s. japonicum. this study showed that most of the identified differentially expressed genes (degs) between water buffalo and yellow cattle pre- and post-infection were ... | 2015 | 26125181 |
| b10 cells induced by schistosoma japonicum soluble egg antigens modulated regulatory t cells and cytokine production of t cells. | a distinct subset of b cells, also known as regulatory b cells, can negatively regulate t cell immune responses, but the role of these cells in schistosomiasis has not been clarified. soluble egg antigen (sea) and soluble adult worm antigen preparation (swap), which are two important antigen sources during schistosoma japonicum infection, both can induce th1, th2, th17, and treg cells and the corresponding cytokines. however, whether they can induce the production of regulatory b cells and the r ... | 2015 | 26149531 |
| antibody signatures reflect different disease pathologies in patients with schistosomiasis due to schistosoma japonicum. | infection with schistosoma japonicum causes high levels of pathology that is predominantly determined by the cellular and humoral response of the host. however, the specific antibody response that arises during the development of disease is largely undescribed in asian schistosomiasis-endemic populations. a schistosome protein microarray was used to compare the antibody profiles of subjects with acute infection, with early or advanced disease associated with severe pathology, with chronic infect ... | 2016 | 26150545 |
| evidence that rhesus macaques self-cure from a schistosoma japonicum infection by disrupting worm esophageal function: a new route to an effective vaccine? | rhesus macaques are unusual among schistosome hosts, self-curing from an established infection and thereafter manifesting solid immunity against a challenge, an ideal model for vaccine development. previously, the immunological basis of self-cure was confirmed; surviving worms had ceased feeding but how immunological pressure achieved this was unclear. the schistosome esophagus is not simply a conduit for blood but plays a central role in its processing. secretions from the anterior and posterio ... | 2015 | 26161644 |
| advanced schistosomiasis reappeared after curing seemingly being cured for over 20 years and without known history of reexposure to schistosoma japonicum. | schistosomiasis is an intravascular disease caused by parasitic trematode worms of the genus schistosoma. it is estimated that more than 200 million people are infected in the world, and 800 million are at risk of infection. the main lesions are due to eggs trapping in tissue which can lead a series of pathogenic effect relative to immune response. therefore, killing and eradicating eggs in tissue is often the target to treat schistosomiasis. here, we report 75 patients who being cured for over ... | 2015 | 26174487 |
| the formation of egg granulomas in the spleens of mice with late schistosoma japonicum infection alters splenic morphology. | splenomegaly is a characteristic symptom of schistosome infection. unlike the well known hepatic pathology of schistosomiasis, splenomegaly has received little scientific research and is generally considered to be a non-specific congestion caused by increased blood pressure within the venous sinuses. moreover, to date, few studies have reported the deposition of schistosome eggs in the spleen. in a previous study, however, we observed that prolonged s. japonicum infections destroyed the structur ... | 2015 | 26178192 |
| adenovirus-mediated over-expression of septin4 ameliorates hepatic fibrosis in mouse livers infected with schistosoma japonicum. | septin4 (sept4) belongs to septin family and may be involved in apoptosis, vesicle trafficking and other cell processes. in this study, we attempted to investigate the effect of sept4 in hepatic fibrosis induced by schistosoma japonicum. icr mice infected with s. japonicum for 12weeks were treated with pbs, ad-ctr and ad-sept4, respectively. all mice were killed at 2weeks after injection, and the changes in the fibrotic livers were detected via h&e staining, sirius red staining, qrt-pcr, western ... | 2015 | 26190030 |
| tissue transglutaminase-regulated transformed growth factor-β1 in the parasite links schistosoma japonicum infection with liver fibrosis. | transforming growth factor (tgf-β1) is among the strongest factors of liver fibrogenesis, but its association with schistosoma-caused liver fibrosis is controversial. tissue transglutaminase (ttg) is the principal enzyme controlling tgf-β1 maturation and contributes to sj-infected liver fibrosis. here we aim to explore the consistency between ttg and tgf-β1 and tgf-β1 source and its correlation with liver fibrosis after sj-infection. tgf-β1 was upregulated at weeks 6 and 8 upon liver fibrosis in ... | 2015 | 26199461 |
| circulating mirnas: potential novel biomarkers for hepatopathology progression and diagnosis of schistosomiasis japonica in two murine models. | schistosomiasis remains a major public health issue, with an estimated 230 million people infected worldwide. novel tools for early diagnosis and surveillance of schistosomiasis are currently needed. elevated levels of circulating micrornas (mirnas) are commonly associated with the initiation and progression of human disease pathology. hence, serum mirnas are emerging as promising biomarkers for the diagnosis of a variety of human diseases. this study investigated circulating host mirnas commonl ... | 2015 | 26230095 |
| comparative phylogenetic studies on schistosoma japonicum and its snail intermediate host oncomelania hupensis: origins, dispersal and coevolution. | schistosoma japonicum causes major public health problems in china and the philippines; this parasite, which is transmitted by freshwater snails of the species oncomelania hupensis, causes the disease intestinal schistosomiasis in humans and cattle. researchers working on schistosoma in africa have described the relationship between the parasites and their snail intermediate hosts as coevolved or even as an evolutionary arms race. in the present study this hypothesis of coevolution is evaluated ... | 2015 | 26230619 |
| molecular docking to explore the possible binding mode of potential inhibitors of thioredoxin glutathione reductase. | praziquantel (pzq) is the treatment of choice for schistosomiasis, one of the most important but neglected tropical diseases. recently, however, schistosoma have exhibited reduced susceptibility to pzq, and an urgent need to develop new drugs to treat schistosomiasis has emerged. thioredoxin glutathione reductase (tgr) plays a crucial role in the redox balance of the parasite, combining glutaredoxin (grx), glutathione reductase and thioredoxin reductase (tr) activities. several compounds, includ ... | 2015 | 26239395 |
| [cloning, expression and immunodiagnostic evaluation of the fasciola gigantica thioredoxin peroxidase]. | to immunoscreen the gene encoding thioredoxin peroxidase (tpx) from a cdna library made from adult fasciola gigantica worms, clone and express the gene, and evaluate the immunodiagnostic value of tpx recombinant protein. | 2015 | 26245114 |
| [plasma metabolism and protective effect of oral administration of niclosamide on schistosoma japonicum cercarial invasion in mice]. | to study the metabolism of niclosamide in plasma, and the protective effect of its oral administration on schistosoma japonicum cercarial invasion in mice. | 2015 | 26245118 |
| rapid detection and identification of four major schistosoma species by high-resolution melt (hrm) analysis. | schistosomiasis, caused by blood flukes belonging to several species of the genus schistosoma, is a serious and widespread parasitic disease. accurate and rapid differentiation of these etiological agents of animal and human schistosomiasis to species level can be difficult. we report a real-time pcr assay coupled with a high-resolution melt (hrm) assay targeting a portion of the nuclear 18s rdna to detect, identify, and distinguish between four major blood fluke species (schistosoma japonicum, ... | 2015 | 26253799 |
| development of paramyosin as a vaccine candidate for schistosomiasis. | schistosomiasis, caused by three principal species of diecious trematodes (flatworms), currently afflicts over 250 million individuals, results in an estimated 2-15% chronic disability, and contributes to poor health and economic stagnation in endemic areas. although schistosomiasis is effectively treated with praziquantel, rapid reinfection with rebound morbidity precludes effective control based on chemotherapy alone and justifies current efforts to develop vaccines for these parasites. paramy ... | 2015 | 26257728 |
| why the radiation-attenuated cercarial immunization studies failed to guide the road for an effective schistosomiasis vaccine: a review. | schistosomiasis is a debilitating parasitic disease caused by platyhelminthes of the genus schistosoma, notably schistosoma mansoni, schistosoma haematobium, and schistosoma japonicum. pioneer researchers used radiation-attenuated (ra) schistosome larvae to immunize laboratory rodent and non-human primate hosts. significant and reproducible reduction in challenge worm burden varying from 30% to 90% was achieved, providing a sound proof that vaccination against this infection is feasible. extensi ... | 2015 | 26257924 |
| an il-13 promoter polymorphism associated with liver fibrosis in patients with schistosoma japonicum. | the aim of this study was to determine whether two polymorphisms in the gene encoding il13 previously associated with schistosoma hematobium (s. hematobium) and s. mansoni infection are associated with s. japonicum infection. single nucleotide polymorphisms (snps) rs1800925 (il13/-1112c>t) and rs20541 (il13r130q) were genotyped in 947 unrelated individuals (307 chronically infected, 339 late-stage with liver fibrosis, 301 uninfected controls) from a schistosomiasis-endemic area of hubei province ... | 2015 | 26258681 |
| [preliminary study on pro-apoptotic gene bad of schistosoma japonicum]. | to understand the characteristics of pro-apoptotic gene sjbad of schistosoma japonicum, such as its biology, immunology, and transcriptional expression, and evaluate its potential of the recombinant protein as a vaccine candidate for schistosomiasis. | 2015 | 26263773 |
| [pathology and pathogenesis of liver fibrosis induced by schistosoma japonicum]. | schistosomiasis is a widely distributed parasitic zoonoses that threatens human' s health and social economic development. china is one of the most endemic countries in the world. schistosoma egg are mainly lodged in the liver and intestinal tissues. there, the eggs induce a granulomatous host immune response largely characterized by lymphocytes, eosinophils, and alternatively activated macrophages. the process of granuloma formation induces chronic inflammation that leads to liver fibrosis acco ... | 2015 | 26263795 |
| schistosoma japonicum protein sjp40 inhibits tgf-β1-induced activation of hepatic stellate cells. | sjp40 is a major egg antigen of schistosoma japonicum. in the present study, the authors investigated the effect of sjp40 in vitro on transforming growth factor-β1 (tgf-β1)- stimulated hepatic stellate cells (hscs). lx-2, an immortalized human hsc line, was treated with purified recombinant sjp40 (rsjp40) in the presence or absence of tgf-β1. quantitative real-time polymerase chain reaction and western blot analysis were performed to determine messenger ribonucleic acid and protein of fibrogenic ... | 2015 | 26268567 |
| multi-host transmission dynamics of schistosomiasis and its optimal control. | in this paper we formulate a dynamical model to study the transmission dynamics of schistosomiasis in humans and snails. we also incorporate bovines in the model to study their impact on transmission and controlling the spread of schistosoma japonicum in humans in china. the dynamics of the model is rigorously analyzed by using the theory of dynamical systems. the theoretical results show that the disease free equilibrium is globally asymptotically stable if r0 < 1, and if r0 > 1 the system has ... | 2015 | 26280190 |
| expression and characterization of a phospholipid hydroperoxide glutathione peroxidase gene in schistosoma japonicum. | phospholipid hydroperoxide glutathione peroxidase (phgpx, gpx4) is a major antioxidant enzyme, which plays unique roles in the protection of cells against oxidative stress by catalysing reduction of lipid hydroperoxides. we isolated and characterized a full-length cdna sequence encoding gpx gene from a blood fluke, schistosoma japonicum (designated sjgpx), which contained an in-frame tga codon for selenocysteine (sec) and a concurrent sec insertion sequence in its 3'-untranslated region. protein ... | 2015 | 26283515 |
| distribution of peripheral memory t follicular helper cells in patients with schistosomiasis japonica. | schistosomiasis is a helminthic disease that affects more than 200 million people. an effective vaccine would be a major step towards eliminating the disease. studies suggest that t follicular helper (tfh) cells provide help to b cells to generate the long-term humoral immunity, which would be a crucial component of successful vaccines. thus, understanding the biological characteristics of tfh cells in patients with schistosomiasis, which has never been explored, is essential for vaccine design. | 2015 | 26284362 |
| comparative analysis of transcriptional profiles of adult schistosoma japonicum from different laboratory animals and the natural host, water buffalo. | schistosomiasis is one of the most widely distributed parasitic diseases in the world. schistosoma japonicum, a zoonotic parasite with a wide range of mammalian hosts, is one of the major pathogens of this disease. although numerous studies on schistosomiasis japonica have been performed using laboratory animal models, systematic comparative analysis of whole-genome expression profiles in parasites from different laboratory animals and nature mammalian hosts is lacking to date. | 2015 | 26285138 |
| cloning, expression and enzymatic characterization of 3-phosphoglycerate kinase from schistosoma japonicum. | in the present study, a full-length cdna encoding the schistosoma japonicum 3-phosphoglycerate kinase (sjpgk) with an open reading frame of 1251 bp was isolated from 42-day-old (42-d) schistosome cdnas. real-time quantitative reverse transcription pcr analysis revealed that sjpgk was expressed in all investigated developmental stages and at a higher transcript levels in 21- and 42-d worms. moreover, the sjpgk mrna level was significantly downregulated in 10-d schistosomula from wistar rats (non- ... | 2015 | 26299245 |
| non-immune immunoglobulins shield schistosoma japonicum from host immunorecognition. | schistosomiasis is a major human parasitic disease with a global impact. schistosoma japonicum, the most difficult to control, can survive within host veins for decades. mechanisms of immune evasion by the parasite, including antigenic variation and surface masking, have been implicated but not well defined. in this study, we defined the immunoglobulin-binding proteomes of s. japonicum using human igg, igm, and ige as the molecular bait for affinity purification, followed by protein identificati ... | 2015 | 26299686 |
| ecological model to predict potential habitats of oncomelania hupensis, the intermediate host of schistosoma japonicum in the mountainous regions, china. | schistosomiasis japonica is a parasitic disease that remains endemic in seven provinces in the people's republic of china (p.r. china). one of the most important measures in the process of schistosomiasis elimination in p.r. china is control of oncomelania hupensis, the unique intermediate host snail of schistosoma japonicum. compared with plains/swamp and lake regions, the hilly/mountainous regions of schistosomiasis endemic areas are more complicated, which makes the snail survey difficult to ... | 2015 | 26305881 |
| protective effect of the ω-3 polyunsaturated fatty acids on the schistosomiasis liver fibrosis in mice. | this study aims to observe the effect of ω-3 polyunsaturated fatty acids on initiation and elimination of the schistosomiasis inflammatory response and liver fibrosis. the mice infected with the cercariae of schistosoma japonicum (20 ± cercarie per mice) were separated randomly into several groups. after 60 days, liver tissue samples of all mice were sectioned. hematoxylin-eosin (he) staining, masson staining, the enzyme-linked immunosorbent assay (elisa), and flow cytometry (fcm) were performed ... | 2015 | 26309610 |
| gdcl3 attenuates schistosomiasis japonicum egg-induced granulomatosis accompanied by decreased macrophage infiltration in murine liver. | early-stage hepatic granuloma and advanced-stage fibrosis are important characteristics of schistosomiasis. the direct consequences of gadolinium chloride (gdcl3) in egg-induced granuloma formation have not been reported, although gdcl3 is known to block the macrophages. in present study, mice were infected with 15 schistosoma japonicum (s. japonicum) cercariae and treated with gdcl3 (10 mg/kg body weight) twice weekly from day 21 to day 42 post-infection during the onset of egg-laying towards e ... | 2015 | 26317423 |
| the characteristics of nk cells in schistosoma japonicum-infected mouse spleens. | natural killer (nk) cells are classic innate immune cells that play roles in many types of infectious disease. recently, some new characteristics of nk cells were discovered. in this study, c57bl/6 mice were infected with schistosoma japonicum for 5-6 weeks and lymphocytes were isolated from the spleen to detect some of the nk cell characteristics by multiparametric flow cytometry. the results revealed that the s. japonicum infection induced a large amount of nk cells, although the percentage of ... | 2015 | 26319521 |
| evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis. | early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. previously we identified six novel antigens with potential as diagnostic markers for human schistosoma japonicum infections. in this study, we evaluated these antigens as candidate biomarkers for the early diagnosis of schistosomiasis in mice and rabbits. | 2015 | 26338369 |
| significance of higher drug concentration in erythrocytes of mice infected with schistosoma japonicum and treated orally with mefloquine at single doses. | the purpose of the present study is to understand the pharmacokinetic feature of mefloquine measured by erythrocytes and plasma in schistosoma japonicum (s. j.)-infected mice and non-infected mice after oral administration of the drug at single doses. a high-performance liquid chromatography (hplc) method was used to measure the plasma and erythrocyte concentrations of mefloquine at varying intervals posttreatment. our results demonstrated that in non-infected mice treated orally with mefloquine ... | 2015 | 26341799 |
| detecting genotyping errors at schistosoma japonicum microsatellites with pedigree information. | schistosomiasis japonica remains a major public health problem in china. integrating molecular analyses, such as population genetic analyses, of the parasite into the on-going surveillance programs is helpful in exploring the factors causing the persistence and/or spread of schistosoma japonicum. however, genotyping errors can seriously affect the results of such studies, unless accounted for in the analyses. | 2015 | 26350750 |
| proteomic analysis on cercariae and schistosomula in reference to potential proteases involved in host invasion of schistosoma japonicum larvae. | schistosomiasis is a parasitic zoonosis posing great threat to human health. the infection is acquired by larval cercariae penetrating host skin and transforming into juveniles, schistosomula. proteolytic enzymes secreted from the cercarial acetabular glands are known to aid to the skin penetration, but molecular mechanisms remain largely unclear. to profile the protein composition and identify potential invasive proteases, we developed a new method for simulating cercarial transformation and co ... | 2015 | 26370134 |
| [cloning, expression of gene sjost48 from schistosoma japonicum and evaluation of the immunoprotective efficacy of rsjost48 in mice]. | to identify sjchgc01743 gene of schistosoma japonicum and evaluate the potential of the recombinant protein as a new vaccine candidate for schistosomiasis, polymerase chain reaction (pcr) technique was used to amplify the cdna of the gene and real-time rt-pcr was used to analyze the transcription profiles of sjchgc01743 at different development stages. recombinant plasmid was successfully constructed and transformed into competent escherichia coli bl21 (de3). then the recombinant protein was exp ... | 2015 | 26380407 |
| transcriptome bioinformatical analysis of vertebrate stages of schistosoma japonicum reveals alternative splicing events. | alternative splicing is a molecular process that contributes greatly to the diversification of proteome and to gene functions. understanding the mechanisms of stage-specific alternative splicing can provide a better understanding of the development of eukaryotes and the functions of different genes. schistosoma japonicum is an infectious blood-dwelling trematode with a complex lifecycle that causes the tropical disease schistosomiasis. in this study, we analyzed the transcriptome of schistosoma ... | 2015 | 26407301 |
| heat shock protein 60 in eggs specifically induces tregs and reduces liver immunopathology in mice with schistosomiasis japonica. | parasitic helminths need to suppress the host immune system to establish chronic infections. paradoxically, immunosuppression induced by the worm also benefits the host by limiting excessive inflammation and tissue damage, which remains the major cause leading to serious morbidity and mortality. regulatory t cells (tregs) are key immune regulators of this mutualism. the successive rise in tregs during schistosome infection plays a critical role in immunoregulation. we and others previously showe ... | 2015 | 26418003 |
| assessment of the diagnostic efficacy of enolase as an indication of active infection of schistosoma japonicum. | schistosomiasis is a common zoonoses affecting humans. the atypical clinical symptoms, low morbidity, and low degree of infection impede diagnosis and assessment of epidemics. detecting circulating antigens from adult worms in patients' body fluids should be diagnostically superior to examining eggs in feces. herein, the excretory-secretory proteins of adult worms were analyzed by using 2-d protein electrophoresis and mass spectrometry. the schistosoma japonicum enolase (sj enolase) was identifi ... | 2016 | 26420425 |
| sjca8, a calcium-binding protein from schistosoma japonicum, inhibits cell migration and suppresses nitric oxide release of raw264.7 macrophages. | schistosomiasis is considered second only to malaria as the most devastating parasitic disease in tropical countries. schistosome cercariae invade the host by penetrating the skin and migrate though the lungs and portal circulation to their final destination in the hepatic portal system and eventually the mesenteric veins. previous studies have shown that the cytotoxic pathways that target schistosomulum in the lung-stage involve nitric oxide (no) produced by macrophages. by contrast, skin-stage ... | 2015 | 26445908 |
| excretory/secretory proteome of 14-day schistosomula, schistosoma japonicum. | schistosomiasis remains a serious public health problem, with 200 million people infected and 779 million people at risk worldwide. the schistosomulum is the early stage of the complex lifecycle of schistosoma japonicum in their vertebrate hosts, and is the main target of vaccine-induced protective immunity. excretory/secretory (es) proteins play a major role in host-parasite interactions and es protein compositions of schistosomula of s. japonicum have not been characterized to date. in the pre ... | 2016 | 26453986 |
| local antiglycan antibody responses to skin stage and migratory schistosomula of schistosoma japonicum. | schistosomiasis is a tropical disease affecting over 230 million people worldwide. although effective drug treatment is available, reinfections are common, and development of immunity is slow. most antibodies raised during schistosome infection are directed against glycans, some of which are thought to be protective. developing schistosomula are considered most vulnerable to immune attack, and better understanding of local antibody responses raised against glycans expressed by this life stage mi ... | 2015 | 26459512 |
| a novel coagulation inhibitor from schistosoma japonicum. | little is known about the molecular mechanisms whereby the human blood fluke schistosoma japonicum is able to survive in the host venous blood system. protease inhibitors are likely released by the parasite enabling it to avoid attack by host proteolytic enzymes and coagulation factors. interrogation of the s. japonicum genomic sequence identified a gene, sjki-1, homologous to that encoding a single domain kunitz protein (sjp_0020270) which we expressed in recombinant form in escherichia coli an ... | 2015 | 26463744 |
| effect of photoperiod change on chronobiology of cercarial emergence of schistosoma japonicum derived from hilly and marshy regions of china. | the chronobiology of cercarial emergence appeared to be a genetically controlled behavior, adapted to definitive host species, for schistosome. however, a few physiological and ecological factors, for example the change of photoperiod, were reported to affect the rhythmic emergence of cercariae. therefore, the effect of photoperiod change on cercarial emergence of two schistosoma japonicum isolates, the hilly and the marshland, was investigated. four shedding experiments each under a different p ... | 2015 | 26484419 |
| molecular cloning and characterization of a phospholipid hydroperoxide glutathione peroxidase gene from a blood fluke schistosoma japonicum. | phospholipid hydroperoxide glutathione peroxidase (phgpx) is a major antioxidant enzyme and plays critical roles in the protection of cells against oxidative stress by catalysing reduction of lipid hydroperoxides. a full-length cdna sequence corresponding to gpx gene from schistosoma japonicum (designated sjgpx) was isolated and characterized. sjgpx contained an in-frame tga codon for selenocysteine (sec) and a concurrent sec insertion sequence in its 3'-untranslated region. protein encoded by s ... | 2015 | 26484892 |
| synthesis, bioactivity evaluation, and toxicity assessment of novel salicylanilide ester derivatives as cercaricides against schistosoma japonicum and molluscicides against oncomelania hupensis. | a series of novel salicylanilide ester derivatives were synthesized, characterized, and evaluated for cercaricidal potential against schistosoma japonicum and molluscicidal potential against oncomelania hupensis. four derivatives exhibited remarkable cercaricidal activity superior to that of niclosamide. among them, the most active compound, 4-chloro-2-((2-methoxy-4-nitrophenyl)carbamoyl)phenyl 4-methoxybenzoate (compound 4c), showed a marked minimum effective cercaricidal concentration as low a ... | 2015 | 26503661 |
| [integration and demonstration of key techniques in surveillance and forecast of schistosomiasis in jiangsu province i layout and effect of the demonstration sites for schistosomiasis surveillance and forecast]. | to explore and integrate the key techniques used in the surveillance and forecast of schistosomiasis in the water regions along the yangtze river, so as to provide technical support for identifying rapidly the risk of schistosomiasis transmission and implementing control measures targeting the risk. | 2015 | 26510350 |
| [molecular characteristics and rna interference efficiency of schistosoma japonicum sj79 gene]. | to study the structural features and characteristics of a novel gene schistosoma japonicum 79 (sj79), and observe its effect of rna interference (rnai) , so as to provide the experimental basis for its further function study and mechanism study of anti reproductive development of schistosome. | 2015 | 26510359 |
| [recombinant expression of schistosoma japonicum fructose-1, 6-bisphos- phate aldolase and its expression in different developmental stages of s. japonicum]. | to clone, express and purify schistosoma japonicum fructose-1, 6-bisphosphate aldolase (sjfbpa) in e. coli and observe its expression in different developmental stages of s. japonicum. | 2015 | 26510360 |
| efficacy and safety of praziquantel for the treatment of human schistosomiasis during pregnancy: a phase 2, randomised, double-blind, placebo-controlled trial. | despite who recommendations to offer pregnant women treatment with praziquantel, many nations continue to withhold treatment, awaiting data from controlled trials addressing safety and efficacy. the objectives of this study were to assess whether treatment of pregnant women with schistosomiasis at 12-16 weeks gestation leads to improved maternal and newborn outcomes and to collect maternal and newborn safety data. | 2016 | 26511959 |
| high-throughput sequencing of rnas isolated by cross-linking immunoprecipitation (hits-clip) reveals argonaute-associated micrornas and targets in schistosoma japonicum. | schistosomiasis, caused by schistosomes, is one of the most prevalent and serious parasitic diseases in tropical and subtropical countries. this pathogen has a complex life cycle and harbors a unique repertoire of genes expressed at different life-stages. understanding the gene regulation of schistosomes will contribute to identification of novel drug targets and vaccine candidates. some conserved and novel micrornas (mirnas) have been identified in schistosomes as key transcriptional and post-t ... | 2015 | 26577460 |
| exploring molecular variation in schistosoma japonicum in china. | schistosomiasis is a neglected tropical disease that affects more than 200 million people worldwide. the main disease-causing agents, schistosoma japonicum, s. mansoni and s. haematobium, are blood flukes that have complex life cycles involving a snail intermediate host. in asia, s. japonicum causes hepatointestinal disease (schistosomiasis japonica) and is challenging to control due to a broad distribution of its snail hosts and range of animal reservoir hosts. in china, extensive efforts have ... | 2015 | 26621075 |
| characterization of vamp2 in schistosoma japonicum and the evaluation of protective efficacy induced by recombinant sjvamp2 in mice. | the outer-tegument membrane covering the schistosome is believed to maintain via the fusion of membranous vesicles. fusion of biological membranes is a fundamental process in all eukaryotic cells driven by formation of trans-snare (soluble n-ethylmaleimide-sensitive factor attachment protein receptor) complexes through pairing of vesicle associated v-snares (vamp) with complementary t-snares on target membranes. the purpose of this study was to characterize schistosoma japonicum vesicle-associat ... | 2015 | 26641090 |
| development of a schistosomiasis vaccine. | schistosomiasis is a neglected tropical disease (ntd) of public health importance. despite decades of implementation of mass praziquantel therapy programs and other control measures, schistosomiasis has not been contained and continues to spread to new geographic areas. a schistosomiasis vaccine could play an important role as part of a multifaceted control approach. with regards to vaccine development, many biological bottlenecks still exist: the lack of reliable surrogates of protection in hum ... | 2016 | 26651503 |
| mice lack of lrg-47 display the attenuated outcome of infection with schistosoma japonicum. | interferon-inducible gtpase lrg-47 (also named immune-related gtpase m, irgm1) is a member of the p47 gtpase family that has been shown to regulate host resistance to intracellular pathogens. little knowledge has been known about the role of lrg-47 in host's responses to extracellular pathogens. to investigate possible roles of lrg-47 in the course of schistosoma japonicum infection, lrg-47-deficient (lrg-47(-/-)) and wild-type (wt) mice were challenged with cercariae of s. japonicum, and the ce ... | 2016 | 26660918 |
| [preliminary genetic and functional analyses of schistosoma japonicum chymotrypsin-like protease]. | to investigate the gene expression, localization and potential functions of schistosoma japonicum chymotrypsin-like protease (sjctrl) in the host, and evaluate its potential immune-protection efficacy against s. japonicum infection in mice. | 2015 | 26672212 |
| [dynamic alteration of cd154/cd40 and its effects on th1/th2 polarization in inducible co-stimulator ligand knockout mice infected with schistosoma japonicum]. | to analyze effect on the cd154-cd40 signaling pathway and th1/th2 polarization by deficient inducible co-stimulator (icos)-icos ligand (icosl) signaling in mice infected with schistosoma japonicum. | 2015 | 26679647 |
| generation of a novel bacteriophage library displaying scfv antibody fragments from the natural buffalo host to identify antigens from adult schistosoma japonicum for diagnostic development. | the development of effective diagnostic tools will be essential in the continuing fight to reduce schistosome infection; however, the diagnostic tests available to date are generally laborious and difficult to implement in current parasite control strategies. we generated a series of single-chain antibody fv domain (scfv) phage display libraries from the portal lymph node of field exposed water buffaloes, bubalus bubalis, 11-12 days post challenge with schistosoma japonicum cercariae. the select ... | 2015 | 26684756 |
| co-dispersal of the blood fluke schistosoma japonicum and homo sapiens in the neolithic age. | the global spread of human infectious diseases is of considerable public health and biomedical interest. little is known about the relationship between the distribution of ancient parasites and that of their human hosts. schistosoma japonicum is one of the three major species of schistosome blood flukes causing the disease of schistosomiasis in humans. the parasite is prevalent in east and southeast asia, including the people's republic of china, the philippines and indonesia. we studied the co- ... | 2015 | 26686813 |
| a rapid monitoring and evaluation method of schistosomiasis based on spatial information technology. | thanks to spatial information technologies (sits) such as remote sensing (rs) and geographical information system (gis) that are being quickly developed and updated, sits are being used more widely in the public health field. the use of sits to study the characteristics of the temporal and spatial distribution of schistosoma japonicum and to assess the risk of infection provides methods for the control and prevention of schistosomiasis japonica has gradually become a hot topic in the field. the ... | 2015 | 26703635 |
| sjtat-tpi facilitates adaptive t-cell responses and reduces hepatic pathology during schistosoma japonicum infection in balb/c mice. | schistosomiasis is a kind of parasitic zoonoses which causes serious damage to public health and social development. china is one of the countries most affected by schistosoma japonicum and an effective vaccine is still needed. in this study, we adopted tat-mediated protein transduction technology to investigate the impact of different antigen presented approaches on host's immune response and the potential protection against schistosoma japonicum infection. | 2015 | 26714844 |
| therapeutic potential of recombinant cystatin from schistosoma japonicum in tnbs-induced experimental colitis of mice. | helminth infections and their components have been shown to have a protective effect on autoimmune diseases. the isolated purified protein from schisotosoma japonicum and its potential therapeutic effect on trinitrobenzene sulfonic acid (tnbs)-induced colitis could provide an alternative way to treat inflammatory bowel disease (ibds). | 2016 | 26728323 |
| foxp3+ regulatory t cells in hepatic fibrosis and splenomegaly caused by schistosoma japonicum: the spleen may be a major source of tregs in subjects with splenomegaly. | schistosoma eggs cause chronic liver inflammation and a complex disease characterized by hepatic fibrosis (hf) and splenomegaly (splm). foxp3+ tregs could regulate inflammation, but it is unclear where these cells are produced and what roles they play in human schistosomiasis. we investigated blood and spleen foxp3+ tregs in chinese fishermen with lifelong exposure to schistosoma japonicum and various degrees of liver and spleen disease. foxp3+ tregs accounted for 4.3% of cd4+ t cells and 41.2% ... | 2016 | 26731721 |
| [study on immunologic function of thioredoxin glutathione reductase from schistosoma japonicum]. | to study the immunogenicity and the immuno-protection of thioredoxin glutathione reductase from schistosomajaponicum (sjtgr) against schistosome infection in mice. | 2015 | 26767257 |
| [effect of icos signaling on cd154/cd40 expressions in mice infected with schistosoma japonicum]. | to explore the effect of icos signaling on the cd154/cd40 expressions and immunopathology in mice infected with schistosoma japonicum. | 2015 | 26767258 |
| oxadiazole-2-oxides may have other functional targets, in addition to sjtgr, through which they cause mortality in schistosoma japonicum. | schistosomiasis is one of the world's major public health problems. besides praziquantel (pzq), there is currently no other effective treatment against schistosomiasis. the development of new antischistosomal agents to curb the emergence of pzq resistance should be a high priority. oxadiazole-2-oxides have been identified as potential antischistosomal reagents, with thioredoxin glutathione reductase (tgr) being one of their molecular targets. | 2016 | 26791563 |
| the genetic diversity and geographical separation study of oncomelania hupensis populations in mainland china using microsatellite loci. | oncomelania hupensis is the unique intermediate host of schistosoma japonicum, which plays a crucial role in the transmission of schistosomiasis. the endemic area of s. japonicum is strictly consistent with the geographical distribution of o. hupensis. | 2016 | 26791938 |
| temporal genetic diversity of schistosoma japonicum in two endemic sites in china revealed by microsatellite markers. | schistosomiasis is one of the neglected tropical diseases. the causative agent of schistosomiasis in china, schistosoma japonicum, has long been a major public health problem. an understanding of fundamental evolutionary and genetic processes in this species has major implications for its control and elimination. intensive control efforts have greatly reduced the incidence of schistosomiasis in china, but little is known about the genetic consequences of these efforts. | 2016 | 26800884 |
| characterising granuloma regression and liver recovery in a murine model of schistosomiasis japonica. | for hepatic schistosomiasis the egg-induced granulomatous response and the development of extensive fibrosis are the main pathologies. we used a schistosoma japonicum-infected mouse model to characterise the multi-cellular pathways associated with the recovery from hepatic fibrosis following clearance of the infection with the anti-schistosomal drug, praziquantel. in the recovering liver splenomegaly, granuloma density and liver fibrosis were all reduced. inflammatory cell infiltration into the ... | 2016 | 26812024 |
| novel t-cell epitopes on schistosoma japonicum sjp40 protein and their preventive effect on allergic asthma in mice. | allergic asthma is a chronic inflammatory disease mediated by th2 cell immune responses. currently, immunotherapies based on immune deviation are attractive, preventive, and therapeutic strategies for asthma. many studies have shown that intracellular bacterial infections such as mycobacteria and their components can suppress asthmatic reactions by enhancing th1 responses, while helminth infections and their proteins can inhibit allergic asthma via immune regulation. however, some helminth prote ... | 2016 | 26840774 |
| comparative analysis of microrna in schistosomula isolated from non-permissive host and susceptible host. | the reed vole microtus fortis is the only known mammal in which the schistosome is naturally prevented from maturing and schistosome infection does not cause significant pathogenesis. however, the mechanism behind this phenomenon remains unknown. in the present study, solexa deep sequencing technology was used to carry out high-throughput sequencing and comparative analysis of microrna (mirna) between small rna libraries isolated from 10 days oldschistosomula of m. fortis and balb/c mice.in tota ... | 2015 | 26844643 |
| correction: foxp3+ regulatory t cells in hepatic fibrosis and splenomegaly caused by schistosoma japonicum: the spleen may be a major source of tregs in subjects with splenomegaly. | 2016 | 26849557 | |
| antischistosomal activity of n,n'-arylurea analogs against schistosoma japonicum. | although the antischistosomal activities of n,n'-arylurea analogs were reported, systematic structure-activity relationships have not been conducted. in this letter, we reported the design, synthesis and evaluation of 45 n,n'-arylurea analogs. among these prepared compounds, 13 compounds were urea linker modified and 32 were n,n'-arylurea derivatives. the activity evaluation revealed 12 analogs exhibited ic50 values lower than 22.6μm, and 7 of them had ic50 less than 10μm against the juvenile sc ... | 2016 | 26856921 |
| micrornas are involved in the regulation of ovary development in the pathogenic blood fluke schistosoma japonicum. | schistosomes, blood flukes, are an important global public health concern. paired adult female schistosomes produce large numbers of eggs that are primarily responsible for the disease pathology and critical for dissemination. consequently, understanding schistosome sexual maturation and egg production may open novel perspectives for intervening with these processes to prevent clinical symptoms and to interrupt the life-cycle of these blood-flukes. micrornas (mirnas) are key regulators of many b ... | 2016 | 26871705 |
| combined il-12 plasmid and recombinant sjgst enhance the protective and anti-pathology effect of sjgst dna vaccine against schistosoma japonicum. | schistosomiasis is listed as one of most important tropical diseases and more than 200 million people are estimated to be infected. development of a vaccine is thought to be the most effective way to control this disease. recombinant 26-kda glutathione s-transferase (rsjgst) has previously been reported to achieve a worm reduction rate of 42-44%. to improve the efficiency of the vaccine against schistosoma japonicum, we immunized mice with a combination of pcdna vector-encoded 26-kda sjgst (pcdn ... | 2016 | 26891172 |
| forensic study of schistosoma japonicum eggs found in an autopsy case. | 2016 | 26900052 | |
| itraq-based comparative proteomic analysis of excretory-secretory proteins of schistosomula and adult worms of schistosoma japonicum. | schistosomiasis remains a serious public health problem with 200 million people infected and 779 million people at risk worldwide. the schistosomulum and adult worm are two stages of the complex lifecycle of schistosoma japonicum and excretory/secretory proteins (esps) play a major role in host-parasite interactions. in this study, itraq-coupled lc-ms/ms was used to investigate the proteome of esps obtained from schistosomula and adult worms of s. japonicum, and 298 differential esps were identi ... | 2016 | 26915583 |
| [development of colloidal gold immunochromatography assay strip for schistosomiasis diagnosis in domestic animals]. | to develop a quick and easy colloidal gold immunochromatography assay (gica) strip for schistosomiasis diagnosis in domestic animals. | 2015 | 26930931 |
| [preservation of live eggs of schistosoma japonicum]. | to observe the preservation time and activity of miracidium hatching from schistosome eggs preserved in different solutions, so as to obtain the optimal preservation conditions and then provide quality control products for field application. | 2015 | 26930942 |
| [the effect of lipopolysaccharide-induced mouse b cell activation on schistosoma japonicum development]. | to investigate the effect of lipopolysaccharide (lps)-induced b cell activation on the development of schistosoma japonicum. | 2015 | 26931036 |
| exposure versus susceptibility as alternative bases for new approaches to surveillance for schistosoma japonicum in low transmission environments. | currently, schistosomiasis in china provides an excellent example of many of the challenges of moving from low transmission to the elimination of transmission for infectious diseases generally. in response to the surveillance dimension of these challenges, we here explore two strategic approaches to inform priorities for the development of improved methods addressed specifically to schistosomiasis in the low transmission environment. we utilize an individually-based model and the exposure data u ... | 2016 | 26942912 |
| effect of corilagin on the mir-21/smad7/erk signaling pathway in a schistosomiasis-induced hepatic fibrosis mouse model. | this study sought to investigate the anti-fibrotic effect of corilagin via interference with the mir-21/smad7/erk signaling pathway in a schistosomiasis-induced hepatic fibrosis mouse model. mice were infected with schistosoma japonicum cercaria to establish the mouse model of schistosomiasis-induced hepatic fibrosis. at four weeks after infection, the groups were given different medications. the living conditions were observed. real-time pcr was employed to detect the mrna levels of mir-21, sma ... | 2016 | 26946098 |
| schistosoma japonicum hsp60-derived peptide sjmhe1 suppresses delayed-type hypersensitivity in a murine model. | parasite-derived molecules with immunomodulatory properties, which have been optimised during host-parasite co-evolution, exhibit potential applications as novel immunotherapeutics. we have previously demonstrated that schistosoma japonicum hsp60-derived peptide sjmhe1 induces cd4(+)cd25(+) regulatory t-cells (tregs) and that adoptively transferred sjmhe1-induced cd4(+)cd25(+) tregs inhibit delayed-type hypersensitivity (dth) in mice. however, multiple concerns regarding this method render this ... | 2016 | 26971312 |
| an investigation into the potential effects of infrapopulation structure and other sources of sampling error, on population genetic studies of the transmission of schistosoma japonicum (trematoda: digenea). | schistosoma japonicum remains a major challenge to human and animal health. earlier microsatellite-based studies reported possible definitive-host-specific private alleles within s. japonicum, opening the possibility that different definitive hosts might harbour different parasite strains. previous investigations have also detected near-identical multilocus genotypes in populations of adult worms - possibly the result of mutations occurring during the asexual (intramolluscan) phase of clonal exp ... | 2016 | 27000844 |
| effects of γ-interferon on hepatic fibrosis of schistosoma japonicum-infected mice. | to probe the effect of γ-ifn on hepatic fibrosis in schistosomiasis japonica. | 1997 | 27006573 |
| coinfection of schistosoma species with hepatitis b or hepatitis c viruses. | although a considerable number of studies have been undertaken to date, it is still controversial as to whether or not coinfection with schistosomiasis increases the susceptibility to or progression from hepatitis b virus (hbv) or hepatitis c virus (hcv) infection. this review is a closer examination of the key studies conducted on human populations on clinical factors that were published in english between 1975 and january 2015. our review is mainly based on tables containing the salient inform ... | 2016 | 27015949 |
| optimisation of a droplet digital pcr assay for the diagnosis of schistosoma japonicum infection: a duplex approach with dna binding dye chemistry. | schistosomiasis is a chronically debilitating helminth infection with a significant socio-economic and public health impact. accurate diagnostics play a pivotal role in achieving current schistosomiasis control and elimination goals. however, many of the current diagnostic procedures, which rely on detection of schistosome eggs, have major limitations including lack of accuracy and the inability to detect pre-patent infections. dna-based detection methods provide a viable alternative to the curr ... | 2016 | 27021661 |
| nucleic acid detection in the diagnosis and prevention of schistosomiasis. | schistosomiasis is an important zoonotic parasitic disease that causes serious harms to humans and animals. surveillance and diagnosis play key roles in schistosomiasis control, however, current techniques for surveillance and diagnosis of the disease have limitations. as genome data for parasites are increasing, novel techniques for detection incorporating nucleotide sequences are receiving widespread attention. these sensitive, specific, and rapid detection methods are particularly important i ... | 2016 | 27025210 |
| colonic high-grade tubular adenomas associated with schistosoma japonicum. | we reported a case of sigmoid colonic high grade tubular adenomas associated with deposited ova of schistosoma japonicum. a 76-year-old japanese man was referred to our colonoscopy due to a positive fecal occult blood test. he had a medical history of schistosomiasis japonica. the colonoscopy revealed that there were two sigmoid colon polyps, approximately 8 mm in diameter. these were removed by endoscopic mucosal resection (emr). pathological examination revealed high grade tubular adenomas and ... | 2016 | 27050891 |
| taurine drinking ameliorates hepatic granuloma and fibrosis in mice infected with schistosoma japonicum. | in schistosomiasis, egg-induced hepatic granuloma formation is a cytokine-mediated, predominantly cd4(+) th2 immune response that can give rise to hepatic fibrosis. hepatic fibrosis is the main cause of increased morbidity and mortality in humans with schistosome infection. taurine has various physiological functions and hepatoprotective properties as well as anti-inflammatory and immunomodulatory activity. however, little is known about the role of taurine in schistosome egg-induced granuloma f ... | 2016 | 27054062 |
| molecular characterization of serine protease inhibitor isoform 3, smspi, from schistosoma mansoni. | serine protease inhibitors, known as serpins, are pleiotropic regulators of endogenous and exogenous proteases, and molecule transporters. they have been documented in animals, plants, fungi, bacteria, and viruses; here, we characterize a serpin from the trematode platyhelminth schistosoma mansoni. at least eight serpins have been found in the genome of s. mansoni, but only two have characterized molecular properties and functions. here, the function of s. mansoni serpin isoform 3 (smspi) was an ... | 2016 | 27083187 |
| correction: micrornas are involved in the regulation of ovary development in the pathogenic blood fluke schistosoma japonicum. | 2016 | 27093517 |