Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| hydrolysis of di- and trisialo gangliosides in micellar and liposomal dispersion by bacterial neuraminidases. | the hydrolysis of di- and trisialo gangliosides by bacterial neuraminidases was investigated. slow rates of hydrolysis were obtained with micellar dispersions of the pure gangliosides; the rates increased considerably with mixtures of ganglioside and phospholipids, such as phosphatidylcholine or sphingomyelin. the greatest rates of hydrolysis were obtained with mixtures containing 5-10 mol% ganglioside and 90-95% phospholipid. with the aid of the nonpenetrating reagent trinitrobenzenesulfonic ac ... | 1980 | 6243952 |
| studies on a mammalian hepatic binding protein specific for asialoglycoproteins. evidence for receptor recycling in isolated rat hepatocytes. | freshly isolated rat hepatocytes attained maximal ability to bind, internalize, and degrade 125i-asialo-orosomucoid after 5 h in suspension culture at 37 degrees c. comparison of the number and distribution of the asialoglycoprotein binding sites of these cells revealed that 5% (6.7 x 10(4) receptors/cell) were on the external cell membrane with an average residency time of slightly less than 3 min. the remaining 95% were located intracellularly, as determined with detergent-solubilized hepatocy ... | 1980 | 6244303 |
| studies of enzyme-mediated reactions. part 13. stereochemical course of the formation of histamine by decarboxylation of (2s)-histidine with enzymes from clostridium welchii and lactobacillus 30a. | 1980 | 6244319 | |
| commercial milk products and indigenous weaning foods in a rural west african environment: a bacteriological perspective. | two commercially available baby milks, one 'biologically acidified', the other 'non-acidified', and a traditional weaning food, millet gruel, were prepared and stored under village conditions in west africa. increases in total colony count and in number of bacillus cereus, clostridium welchii, staphylococcus aureus and escherichia coli were determined in these products when stored as commonly practised at ambient temperatures over a period of 8 h. poor hygiene during preparation was indicated by ... | 1980 | 6244343 |
| hospitals should do the sick no harm. 2. why are bacteria harmful? | 1980 | 6244533 | |
| hydrolysis of gangliosides in micellar and liposomal dispersion by bacterial neuraminidases. | aqueous dispersions of pure gangliosides contain micelles of these compounds. in this dispersion state, the rates of hydrolysis of the neuraminyl residues by bacterial neuraminidases are slowest. incorporation of gangliosides into mixed dispersion with other lipids or into mixed micelles with bile salts considerably increases the reaction rates. the greatest reaction rates are obtained when di- or trisialogangliosides are incorporated into unilamellar vesicles of lecithin or sphingomyelin. | 1980 | 6244720 |
| clostridium perfringens as a water pollution indicator. | 1980 | 6245283 | |
| [gas gangrene caused by bacteria, other than clostridium, and diabetes mellitus. a study of two cases (author's transl)]. | 1980 | 6245313 | |
| [the incubation period of gas gangrene (author's transl)]. | 1980 | 6245494 | |
| [early diagnosis and treatment of anaerobic infection]. | 1980 | 6245535 | |
| phosphatidylglycerophosphate synthease and phosphatidylserine synthase activites in clostridium perfringens. | cytidine 5'-diphospho (cdp)-1,2-diacyl-sn-glycerol (cdpdiacylglycerol):sn-glycerol-3-phosphate phosphatidyltransferase (ec 2.7.8.5, phosphatidylglycero-p synthase) and cdpdiacylglycerol:l-serine o-phosphatidyltransferase (ec 2.7.8.8, phosphatidylserine synthase) activities were identified in the cell envelope fraction of the gram-positive anaerobe clostridium perfringens. the association of phosphatidylglycero-p synthase and phosphatidylserine synthase with the cell envelope fraction of cell-fre ... | 1980 | 6246064 |
| purification to homogeneity of h blood group beta-galactoside alpha 1 leads to 2 fucosyltransferase from porcine submaxillary gland. | a beta-galactoside alpha 1 leads to 2 fucosyltransferase has been solubilized from porcine submaxillary glands and purified 124,000-fold to homogeneity by repeated affinity chromatography on gdp-hexanolamine agarose. sodium dodecyl sulfate polyacrylamide gel electrophoresis of the purified enzyme revealed two electrophoretic species with apparent mr = 60,000 and 55,000. the two enzyme species have not been completely resolved, but both appear to be active forms of the fucosyltransferase with app ... | 1980 | 6246105 |
| binding of enterotoxin from clostridium perfringens type a to liver cells in vivo and in vitro. the enterotoxin causes membrane leakage. | enterotoxin from clostridium perfringens was shown to retain its biological activity after labelling with 125i. when injected intravenously into mice and rats, most of the radioactivity in the organs was present in the form of intact toxin. studies of the tissue distribution of labelled enterotoxin showed the largest amounts in the liver, where the activity reached a maximum 10--15 min after administration. the highest concentration per g tissue was found in liver and kidneys. the radioactivity ... | 1980 | 6246712 |
| characterization of a new type of glycoprotein saccharides containing polysialosyl sequence. | 1980 | 6246895 | |
| rapid procedures for determination of endo-n-acetyl-alpha-d-galactosaminidase in clostridium perfringens, and of the substrate specificity of exo-beta-d-galactosidases. | culture fluid of clostridium perfringens hydrolyzed the synthetic, chromogenic substrates beta-gal-(1 leads to 3)-alpha-galnac-1 leads to oph and beta-gal-(1 leads to 3)-alpha-galnac-1 leads to oc6h4-no2-o or -p to beta-gal-(1 leads to 3)-galnac and the aglycon. such assays facilitated the characterization and purification of this endo-n-acetyl-alpha-d-galactosaminidase activity. this activity was purified 1200-fold by fractionation with ammonium sulfate and chromatography on columns of sephadex ... | 1980 | 6247065 |
| foodborne disease outbreaks in connecticut during 1977 and 1978. | 1980 | 6247119 | |
| [properties of e. coli, salm. typhimurium and cl. perfringens strains isolated from soil containing bacterial insecticides based on bac. thuringiensis]. | 1980 | 6247250 | |
| [epidemiology of nosocomial clostridial myonecroses (gas gangrene)]. | 1980 | 6247769 | |
| the effect of cadmium on indicator bacteria in sewage. | 1980 | 6247900 | |
| [transferable tetracycline resistance in "clostridium difficile" (author's transl)]. | tetracycline (tc) resistance is transferable from a resistant strain of clostridium difficile to a sensitive strain and this resistance is not curable. resistances to erythromycin and clindamycin are curable but not transferable. these results suggest for these resistances a plasmid determinism. it is shown that a plasmid-mediated tc resistance (pip401) of c. perfringens is also transferable to c. difficile. tc resistance is inducible in c. perfringens and constitutively expressed in c. difficil ... | 1980 | 6247949 |
| sensitive assay for measuring tetracycline levels in gingival crevice fluid. | an increased interest in the clinical use of antibiotics as an adjunct to periodontal therapy has created a need to determine antibiotic concentrations in fluid obtained from the gingival crevice. for this purpose, an increase in sensitivity beyond that possible with current tetracycline assays is essential because sample volumes of gingival fluid typically obtained are less than 0.5 microliter. this report describes the development of an agar-diffusion assay technique capable of measuring the c ... | 1980 | 6247968 |
| reversal of radiation-dependent heat sensitization of clostridium perfringens spores. | the effect of solute concentration on the sensitization of clostridium perfringens spores to heat by ionizing radiation was investigated. as we have shown previously, spores of c. perfringens treated with gamma radiation are now sensitive to subsequent heat treatments than are spores that receive no radiation treatment. when gamma-irradiated spores were heated in the presence of increasing concentrations of glycerol or sucrose, the heat sensitivity induced by irradiation was progressively decrea ... | 1980 | 6247972 |
| relationships between environmental factors, bacterial indicators, and the occurrence of enteric viruses in estuarine sediments. | current standards for evaluation of the public health safety of recreational and shellfish-harvesting waters are based upon bacteriological analysis, but do not include an evaluation of the number of viruses. the objective of this study was to determine the occurrence of enteric viruses in estuarine sediments and to find a relationship, if any, between the presence of viruses in seawater or sediment or both and various biological and physicochemical characteristics of the environment. viruses we ... | 1980 | 6247974 |
| [changes in the serum acid phosphatase activity of guinea pigs poisoned with c1 perfringens type a toxin and a mixture of toxin and a broth culture filtrate]. | it was shown in experiments on guinea pigs that the injection of c1. perfringens type a toxoid induced an increase in acid phosphatase activity of animal blood serum. the action of the toxoid increased under the effect of c1. butyricum cultural filtrate, which gave rise to an earlier enhancement of the specific activity of the enzyme as compared to the injection of the toxoid alone. increased activity of acid phosphatase may play a pathogenetic role in cases of anaerobic infection caused by asso ... | 1980 | 6248148 |
| empyema caused by clostridium perfrigens. | 1980 | 6248609 | |
| fatal clostridium perfringens sepsis; following malignant hemangioendothelioma of scalp. | 1980 | 6248825 | |
| [ecg tracing in electric injuries and subsequent burns complicated by chest contusion and anaerobic septicemia]. | 1980 | 6248996 | |
| the ganglioside content of the milk fat-globule membrane and the mouse mammary-tumour virus isolated from the milk of infected mice. partial characterization of a new disialoganglioside. | the milk fat-globule membrane and the mouse mammary-tumour virus isolated from the milk of infected swiss mice have been investigated for their content in gangliosides. when compared on the lipid phosphorus basis, viral envelope is found to contain more than twice as much lipid-bound sialic acid as fat-globule membrane. the ganglioside patterns of these two structures appear rather similar, except for the occurrence in fat-globule membrane of a low ganglioside homolog, presumably gm2, not detect ... | 1980 | 6249587 |
| synergistic haemolysis test for presumptive identification and differentiation of clostridium perfringens, c. bifermentans, c. sordellii, and c. paraperfringens. | a new test for the presumptive identification of clostridium perfringens, c. bifermentans, c. sordellii, and c. paraperfringens is described. the test is based on the synergistic haemolysis shown by the clostridia and group b streptococci on sheep and human and cacl2-supplemented human blood agar. c. perfringens gave crescent-shaped synergistic lytic zones (7 to over 20 mm in length), and c. paraperfringens usually small-sized (3 mm), bullet-shaped reactions on all three types of media. c. bifer ... | 1980 | 6249855 |
| comparison of methods available for assay of chloramphenicol in clinical specimens. | eight methods for the assay of chloramphenicol in clinical samples were compared with our own modification of a plate diffusion technique using sarcina lutea and yeast extract agar. six of the eight methods were less sensitive than originally reported, and five of them were considered unsuitable for use in clinical microbiology practice. the remaining three methods together with the s. lutea/yeast extract modification were used to assay chloramphenicol in 20 samples of serum. twenty samples of c ... | 1980 | 6249858 |
| [clostridium sepsis as a result of acute gangrenous cholecystitis]. | 1980 | 6250228 | |
| improved method for purification of enterotoxin from clostridium perfringens type a. | the purification procedure of clostridium perfringens type a enterotoxin has been improved. the cell sonic extract was precipitated twice with ammonium sulfate, first 40% saturated to concentrate the enterotoxin and then 15% saturated. the two precipitations were followed by gel filtration on sephadex g-100. the enterotoxin appeared to be homogeneous on 7% polyacrylamide gel electrophoresis after this three-step purification procedure, with a recovery of 56% and a 12.3-fold purification. the sol ... | 1980 | 6250473 |
| [clostridium perfringens infection and necrotising enterocolitis]. | during a four year period (1974--1977) 21 infants died as a result of severe necrotising enterocolitis (n.e.c.). in 9 cases, clostridium perfringens was isolated. when this organism is recovered either from the placenta or from the first meconium and or when the signs of the disease appear within a few days of birth, materno-fetal transmission of the infection may be suspected. the infection occurs most frequently in neonates in whom the gastrointestinal tract was already colonized by clostridiu ... | 1980 | 6250508 |
| the influence of glycosidases and lectins on insulin binding to zajdela hepatoma cells. | 1980 | 6250906 | |
| scanning electronmicroscopy of the jejunum in enteritis necroticans. | intestinal tissue resected at laparotomy from patients in papua new guinea at various clinical stages of enteritis necroticans, locally known as pig-bel, has been examined under the scanning electronmicroscope. evidence obtained from parallel studies of experimental infection in pigs is presented. progressive destruction of the intestinal mucosa was seen during the course of the disease in man. numerous filamentous rods morphologically consistent with the appearance of clostridium perfringens ty ... | 1980 | 6251221 |
| clostridium perfringens wound infection associated with elastic bandages. | clostridium perfringens wound infections were associated with the use of nonsterile elastic outer bandages in diabetic patients who had undergone lower extremity amputation for vascular insufficiency. in each case a second surgical procedure was required. elastic bandages similar to those used in these procedures were found to contain c perfringens and other clostridial species. this report illustrates the need for maintenance of a sterile, nonpermeable inner barrier to prevent transudation of b ... | 1980 | 6251286 |
| effect of oxidizing agents and sulfhydryl group reagents on beta toxin from clostridium perfringens type c. | purified beta toxin from clostridium perfringens type c was inactivated by the oxidizing agents o-iodosobenzoate (oiba), oxidized glutathione, and ferricyanide, and by the sulfhydryl group regents 5,5'-dithio-bis(2-nitrobenzoic acid) (dtnb) and n-ethylmaleimide, iodoacetamide, and iodoacetic acid, causing loss of activity in various degrees depending on the concentration used. the activity of the toxin was not influenced by exposure to 1.0 mm of p-chloromercuribenzoate. the toxin treated by oiba ... | 1980 | 6251344 |
| the control of bacterial contamination in carcass meal with propionic acid. | the effect of the addition of 2, 3, 5, 7 and 10% respectively of propionic acid on known numbers of bacteria added to or contained in carcass meal was examined. escherichia coli was totally inhibited by 2% propionic acid, while 5% of the acid both inhibited salmonella typhimurium and brought about a 74,7% reduction in the total aerobic bacterial count. | 1980 | 6251412 |
| microbiology of the canine nasal cavities. | the anterior and superior regions of canine nares yielded two distinct microbial populations consisting of 25 microbial species. streptococci predominated in the anterior nose; clostridia and staphylococci were also quite prevalent. at least two distinct microorgranisms were cultured from most anterior sites. in contrast, sterile conditions prevailed in the superior nasal regions of at least 50% of the ninety-two animals. various bacillus sp. were recovered from the remainder. | 1980 | 6251533 |
| clostridium perfringens food-borne disease and bacteriological analysis for strain identification. | 1980 | 6251778 | |
| clostridial pleuropulmonary infection. | primary clostridial pleuropulmonary infection occurred in two patients. the pathogenesis of infection was related to aspiration of oropharyngeal contents or hematogenous seeding of infarcted lung tissue. one patient was cured with penicillin; the other died secondary to sepsis and respiratory failure. review of 13 additional cases from the literature demonstrated pulmonary infection due to clostridia to be characterized by lobar or multilobar disease with uniform involvement of the pleura. iatro ... | 1980 | 6251997 |
| clostridium perfringens type a: in vitro system for sporulation and enterotoxin synthesis. | polysomes were isolated from an enterotoxigenic strain of clostridium perfringens during vegetative growth and at 1-h intervals after transfer into duncan-strong sporulation medium. during vegetative growth, about 67% of the ribosomes were in polysomal complexes. this proportion decreased to about 20% during the first 2 h in sporulation medium and then gradually increased to a maximum of 45% at 6 h. ribosomes isolated from cells in vegetative or in sporulation phase could equally translate veget ... | 1980 | 6252194 |
| evaluation of a clostridial alpha-toxin disk test for rapid presumptive identification of group b streptococci. | an alpha-toxin disk test is described in which group b streptococci completed the hemolysis of sheep erythrocytes partially lysed by the alpha-toxin of clostridium perfringens. the test was performed satisfactorily on the sheep blood agar primary isolation plate, as well as on pure cultures. a total of 95% of strains of pure group b streptococci tested produced positive reactions within 5 h, and all were positive after overnight incubation, with patterns of synergistic hemolysis readily distingu ... | 1980 | 6252240 |
| new presumptive identification test for clostridium perfringens: reverse camp test. | the reverse camp test proved to be a highly sensitive test since 97.0% of all clostridium perfringens cultures tested gave an easily discernible positive reaction. | 1980 | 6252247 |
| incidence of increased numbers of clostridium perfringens in the intestinal tract of rats fed xylitol. | sprague-dawley rats were fed diets containing up to 20% xylitol for 49 days. when the rats were fed a xylitol regimen intended to produce adaptation to xylitol, approximately half of the animals adapted to xylitol and remained free from diarrhea during the feeding regimen. the other half did not adapt to xylitol and developed severe and persistent diarrhea accompanied by large volumes of intestinal gas. these non-adapted rats had significantly higher levels of intestinal tract clostridium perfri ... | 1980 | 6252305 |
| ampicillin-induced enterocolitis: implication of toxigenic clostridium perfringens type c. | 1980 | 6252310 | |
| a cluster of septic complications associated with illegal induced abortions. | between august 7 and october 14, 1977, clostridium perfringens organisms were isolated from endometrial and/or blood specimens from 3 women who had been hospitalized in mcallen, texas, after having had illegal induced abortions. one of the women died of clostridial septicemia. a single abortionist was suspected in these 3 cases. the authors investigated the illnesses of these 3 women and those of 6 other women who were hospitalized at some time from january through october 1977 after having had ... | 1980 | 6252522 |
| the effects of clostridium perfringens type a enterotoxin in shetland ponies--clinical, morphologic and clinicopathologic changes. | severe abdominal pain, classic colic signs and hemorrhagic gastro-entero-cecocolitis were induced in three conventional shetland ponies by intravenous injection with clostridium perfringens type a enterotoxin. histological examination showed marked congestion, edema and hemorrhage of the large and small intestine and sloughing of the tips of the intestinal villi. marked vacuolar degeneration of hepatocytes with dilatation of the spaces of disse also was found. clinical changes consisted of sever ... | 1980 | 6252678 |
| [problems of maintaining hygienic and microbiological quality standards in the production of foodstuffs, particularly those of vegetable origin. second report: demands regarding hygiene and microbiology-theory is useless without practice (author's transl)]. | many legal regulations exist on the hygienic-microbiological properties of food products. the 'länder-speiseeisverordnungen' (county ice cream regulations), the 'diät-verordnung' (dietary regulation) and the 'trinkwasser-verordnung' (regulation on potable water) are examples for the frequent inadequacies inherent in these regulations. caution is indicated against any new proposals for the establishment of additional hygienic-microbiological regulations without sufficient practice-related reasoni ... | 1980 | 6252723 |
| [rendering of animal material in the netherlands (author's transl)]. | just before the world war ii rendering of all material of animal origin unfit for human and animal consumption was a fact and was later on regulated by decree in 1942. the rendering act dates from 1957 referring to as "processing into useful product". there is a compulsery rendering which means that withdrawal of the material mentioned from rendering is in general prohibited. material for rendering must be notified. in use is the dry rendering system (atmospheric batch cookers). at the moment th ... | 1980 | 6252724 |
| [computation for the sterilisation process in rendering plants (author's transl)]. | in the present paper a mathematical model for the computation and costing of the sterilisation process in rendering plants has been developed and the computation of the f value in the canning industry has been used as a guide. in this, a test germ with the heat resistance parameters z = 10 degrees c and dr = 10 sec is taken as a guide. if the steam-pressure heating regulation of the german carcass disposal act which prescribes a temperature of 133 degrees c during 20 min after decomposition of t ... | 1980 | 6252725 |
| experimental pigbel: the production and pathology of necrotizing enteritis due to clostridium welchii type c in the guinea-pig. | an animal model for pigbel in man was developed using guinea-pigs. intragastric dosing with growing cultures of clostridium welchii type c only produced necrotic lesions if protease inhibitors were given as well. beta toxin, which is made by the type c organism, causes the intestinal damage and is very easily destroyed by proteases. protease inhibitors in soybean and aprotinin were effectively in inducing disease in animals on a normal diet, while inhibitors in sweet potato, which inhibit only t ... | 1980 | 6252934 |
| binding of clostridium perfringens [125i]enterotoxin to rabbit intestinal cells. | 125i-labeled enterotoxin from clostridium perfringens was utilized to characterize the association of the enterotoxin with cells isolated from rabbit intestine and tissue homogenates from liver, kidney, and brain. the enterotoxin was found to bind in a specific and saturable manner to cells from intestine and to tissue homogenates from liver and kidney but not the brain. detailed studies of the binding were carried out with the ileal epithelial intestinal cells. the rate and amount of binding of ... | 1980 | 6252960 |
| clostridium perfringens in animal disease: a review of current knowledge. | the diseases caused by various types of clostridium perfringens are critically reviewed in the light of current knowledge. particular emphasis is placed on information concerning these diseases in canadian livestock. there are two etiologically clearly-defined acute c. perfringens diseases recognized in canada: hemorrhagic enteritis of the new born calf, caused by c. perfringens type c, and enterotoxemia of sheep, caused by type d. clostridium perfringens type a may play a role as a secondary pa ... | 1980 | 6253040 |
| pathogenesis of necrotising enteritis with special reference to intestinal hypersensitivity reactions. | the aetiological aspects of 83 sporadic cases of necrotising enteritis (ne) have been studied. of 56 cases in which histology of the intestine was possible, eight showed appearances (oedema and local eosinophilia) suggestive of a type i hypersensitivity reaction, while in 37 the appearances were suggestive of a type iii reaction. we suggest that these reactions, which were more common in children and young adults, were initiating factors in the intestinal necrosis. the type iii reactions (submuc ... | 1980 | 6253362 |
| [1h-nmr-spectroscopic evidence for the release of n-acetyl-alpha-d-neuraminic acid as the first product of neuraminidase action (author's transl)]. | the 1h-nmr spectroscopy was used to study the anomeric configuration of n-acetyl-d-neuraminic acid released by the action of neuraminidase. the hydrolysis of neuacalpha 2 leads to 3 gal-beta 1 leads to 4glc (20mm) by the enzymes of clostridium perfringens and arthrobacter ureafaciens (50 mu, 150 mu and 800 mu, respectively) in 50mm na/k-phosphate buffer pd 5.4 was observed by recording the spectra. on the basis of the characteristic signals of the protons at c-3 (alphaneuac: delta[h(3e)] = 2.72, ... | 1980 | 6253376 |
| interaction of streptolysin o from streptococcus pyogenes and theta-toxin from clostridium perfringens with human fibroblasts. | the membrane-damaging properties on human diploid embryonic lung fibroblasts of streptolysin o (from streptococcus pyogenes) and theta-toxin (from clostridium perfringens) were compared. the results are consistent with the suggested mechanism for hemolysis by streptolysin o involving one fixation site and one lytic site of this cytolysin. however, the membrane-damaging activity of the two toxins differed with respect to (i) relative cytolytic activity on human diploid lung fibroblasts compared w ... | 1980 | 6253400 |
| relationship between the increased sensitivity of heat injured clostridium perfringens spores to surface active antibiotics and to sodium chloride and sodium nitrite. | 1980 | 6253431 | |
| regulation of phosphatidylcholine biosynthesis in cultured chick embryonic muscle treated with phospholipase c. | cultures of embryonic chick muscle cells grown in medium containing phospholipase c from clostridium perfringens incorporated [3h]choline into lipid at a rate 3- to 5-fold higher than control cultures. to determine the mechanism by which stimulation of phosphatidylcholine synthesis occurred in phospholipase c-treated cells, activities of enzymes and levels of intermediates in the biosynthetic pathway for phosphatidylcholine were examined. activities of choline kinase, choline phosphotransferase, ... | 1980 | 6253483 |
| hepatitis due to clostridium perfringens in a dog. | 1980 | 6254225 | |
| clostridium perfringens type e enterotoxaemia in rabbits. | 1980 | 6254237 | |
| the isolation of salmonella from jejunal and caecal lymph nodes of slaughtered animals. | one jejunal and one caecal lymph node were sampled from each of 50 cows, 40 yearling cattle, 25 sheep, 20 lambs and 45 pigs after slaughter. salmonella, clostridium perfringens and staphylococcus aureus, all organisms which cause food poisoning in man, were sought by direct plating methods. the samples were also enriched and cultured for salmonella. organisms were cultured from 208 (58%) of the 360 lymph nodes; aerobic plate counts yielded up to 25,000 organisms per gram of tissue, although from ... | 1980 | 6254484 |
| role of coccidia in the occurrence of necrotic enteritis of chickens. | clostridium perfringens type a, eimeria acervulina, and eimeria necatrix were used to produce necrotic enteritis in chickens. the disease was produced in all groups of birds that received feed contaminated with c. perfringens. mortality due to necrotic enteritis was highest (53%) in birds infected with e. acervulina before infection with clostridia. there was a significant difference in mortality rates between birds infected with e. acervulina and birds infected with e. necatrix before infection ... | 1980 | 6254485 |
| [experimental and clinical experiences with the synthetic skin cover of polyvinylalcohol-formaldehyde foam (pva)]. | animal experimental research and clinical experience in polyvinylalcohol formaldehyde foam (pva) as a temporary skin cover: the usefulness of pva as a temporary skin cover of burns and infected wounds was studied in animals. on the back of 20 pigs full thickness burns were produced and wounds were infected, then examined macroscopically, histologically and bacteriologically over a period of 18 days. pva showed a better wound cleansing, a significantly reduced bacterial flora and a more rapid gro ... | 1980 | 6254911 |
| purification and properties of alpha-n-acetylgalactosaminidase from clostridium perfringens. | exo-alpha-n-acetylgalactosaminidase has been purified 8000-fold from clostridium perfringens by gel filtration, ion exchange chromatography, isoelectric precipitation, and negative adsorption on human o type erythrocytes. the resulting enzyme is active at physiological ph and temperature. phenyl glycosides, oligosaccharides, mucins, glycolipids, and cell membranes are substrates for this enzyme. the result of enzyme action on blood type a erythrocytes is the loss of a activity and the simultaneo ... | 1980 | 6254979 |
| clostridium perfringens toxins (type a, b, c, d, e). | 1980 | 6255496 | |
| clostridium infection in mother and infant. | 1980 | 6255793 | |
| relationship between metronidazole metabolism and bactericidal activity. | it has been suggested that the microbicidal effect of metronidazole is mediated by an intermediate in nitro group reduction. we have found that the addition of escherichia coli enhances the lethal effect of metronidazole on bacillus fragilis and suggest that this intermediate may form in one bacteria and kill another. because acetamide forms during the reduction of metronidazole, we examined the possibility that the same partially reduced intermediate in metronidazole reduction may be both an in ... | 1980 | 6255861 |
| preliminary report on serogrouping of indian strains of clostridium perfringens type a. | 1980 | 6256288 | |
| mechanisms of pathogenicity of anaerobic bacteria of clinical interest. | the host factors leading to anaerobic infection and the various models of anaerobic infection, namely direct effect of a single toxin, multiple toxin-aggressin-systems, enterotoxic mechanisms, endogenous infections with non-clostridial anaerobes, and synergistic infections, are reviewed. production of carcinogens and co-carcinogens by anaerobes is also discusssed. | 1980 | 6256292 |
| methods for testing antibiotic sensitivity of anaerobic bacteria. | problems are still encountered in the performance and interpretation of tests of anaerobe sensitivity to antibiotics. a review of the methods currently used was carried out in order to determine factors modifying the activity of antibiotics. the sensitivity of escherichia coli, staphylococcus aureus and clostridium perfringens to various drugs was tested under different conditions (including different culture media and incubation atmospheres). gentamicin and kanamycin showed no activity in tests ... | 1980 | 6256293 |
| the bacteriological quality of minced beef in the u.k. | minced (ground) beef from three supermarkets, three intermediate-sized chain butchers and three small family butchers in each of three geographical areas was examined three times in warm weather and three times in cool. the total viable count (37 and 20 degrees c), numbers of enterobacteriaceae (37 and 17 degrees c), and presumptive coliforms did not differ significantly between shop type or season. statistically significant differences in numbers of faecal streptococci, staphylococcus aureus an ... | 1980 | 6256434 |
| [susceptibility of anaerobic bacteria to cefoxitin (author's transl)]. | nine strains of clostridium perfringens type a and 10 strains of bacteroïdes fragilis s. spec. fragilis, recently isolated from blood culture, were tested for their cefoxitin susceptibility. the mic for clostridium perfringens ranged from 0,6 to 5 mg/l and 1,2 to 10 mg/l for bacteroïdes fragilis. in most cases, the mbc were identical with the mic for clostridium perfringens, but for bact. frag. there was a difference of 2 dilutions in their mbc. cefoxitin activity was not modified by a variation ... | 1980 | 6256880 |
| behavior of pathogenic bacteria in the oyster, crassostrea commercialis, during depuration, re-laying, and storage. | oysters (crassostrea commercials) harvested from major cultivation areas within the state of new south wales, australia, were commonly contaminated with low levels of the food-poisoning organisms bacillus cereus, clostridium perfringens, and vibrio parahaemolyticus. salmonella was found in oysters on only one occasion. these bacteria were cleansed from oysters during oyster purification by re-laying in a non-polluted waterway. oysters were laboratory contaminated to levels in excess 1,000 cells ... | 1980 | 6257164 |
| plasmid detection in a bacteriocinogenic strain of clostridium perfringens. | bacteriocinogenic clostridium perfringens, strain 28, harboured plasmid dna detectable by dye-bouyant density-gradient centrifugation. this plasmid dna was absent from an ultraviolet light cured variant which had simultaneously lost its immunity and ability to produce bacteriocin. agarose gel electrophoresis of the plasmid dna revealed at least six bands but denaturation experiments suggested three plasmids occurring in more than one conformation. electron microscopy revealed three major size di ... | 1980 | 6257358 |
| ultrastructure of sporulating cells of clostridium perfringens type a grown in the presence of raffinose. | seven-hour sporulating cells of clostridium perfringens type a strain nctc 10240 grown in the presence of raffinose versus starch were larger, produced a thicker cortex, and were altered in spore coat structure. strain nctc 8798 possessed an expanded subcoat region and (or) produced coat fragments when grown in the presence of raffinose. | 1980 | 6257363 |
| a survey of clostridium perfringens enterotoxin antibody in human and animal sera in western canada. | sera from human, cattle, sheep, swine, and horse populations in western canada were tested for the presence of clostridium perfringens enterotoxin antibody by the passive hemagglutination (pha) test, supplemented by an immunodiffusion test and by counterimmunoelectrophoresis. a total of 224 human, 345 cattle, 165 sheep, 620 swine, and 768 horse serum samples were examined. low-titer reactions in the pha test were detected in human, cattle, horse, and swine sera, in that order, with no titers dem ... | 1980 | 6257364 |
| the protection role of pyruvate against heat inactivation of n-acetylneuraminate lyase. | 1980 | 6257549 | |
| clostridium perfringens type a enteropathy: studies with a nonenterotoxigenic strain. | 1980 | 6257612 | |
| radioiodination of enterotoxin from clostridium perfringens type a using chloramine t. | 1980 | 6257632 | |
| passive bacteriocin typing of strains of clostridium perfringens type a causing food poisoning for epidemiologic studies. | a passive bacteriocin typing system was developed for use as an epidemiologic tool to study outbreaks of food poisoning caused by clostridium perfringens type a. the 90 strains tested were from england, the united states, india, japan, france, and canada. forty of 74 strains produced bacteriocin when irradiated with ultraviolet light for 40-90 sec. eight bacteriocins were concentrated with ammonium sulfate and titrated on a common indicator strain. these bacteriocins were found to be specific fo ... | 1980 | 6257802 |
| enterotoxaemia involving clostridium perfringens iota toxin in a hysterectomy-derived rabbit colony. | during an explosive outbreak of fatal enteropathic disease involving clostridium perfringens iota (i) toxin, a total of 183 deaths occurred in 18 weeks. the clinical signs and post-mortem findings are reported. examinations for virus, bacillus piliformis and coccidia were negative. clostridium perfringens i toxin was detected in 22 of 27 animals examined (81.5%), but clostridia were not isolated. various treatments were attempted. it is concluded that i toxin and the syndrome described are close ... | 1980 | 6257969 |
| [post-operative meningitis due to obligatory anaerobic bacteria: 3 case reports]. | 1980 | 6258136 | |
| [isolation of the theta-hemolysin of cl. perfringens]. | teta-hemolysine was purified from cl. perfringens strain bp6k 28 as follows: reprecipitation in the isoelectric point of the enzyme with 2 n h2so4 containing 15% nacl, deae cellulose chromatography, gel filtration on sephadex g-100-g75 or bio gel p-60--p-100, rechromatography on deae-sephadex a-50 and affinity chromatography. the biological activity of homogenous teta-hemolysine, estimated by complete hemolysis of human erythrocytes, exceeded 100, 000 theta e over mg protein but the enzyme was h ... | 1981 | 6258318 |
| [the suitability of bioindicators according to din 58948 part 4 for monitoring gas-sterilizers (author's transl)]. | in the federal republic of germany bioindicators according to din 58948, part 4, are generally used for testing the efficacy of ethylene oxide sterilizers. they are based on spores of bacillus subtilis dried in sheep-blood on the bottom of a small test tube. as some authors doubted the resistance of these indicators to ethylene oxide especially in comparison with spore soil and spores of clostridium perfringens, three different test procedures were performed showing that spores of sulfite reduci ... | 1980 | 6258352 |
| demonstration and characterization of partial glyceride specific lipases in pig thyroid plasma membranes. | 1980 | 6258596 | |
| myoelectric activity in the small intestine in response to clostridium perfringens a enterotoxin: correlation with histologic findings in an in vivo rabbit model. | 1981 | 6259015 | |
| [in vitro activity of cefotaxime on anaerobes (author's transl)]. | the comparative activity of cefotaxime, cefazolin, cefamandole and cefoxitin was studied on 80 strains of anaerobic bacterial species: 14 clostridia, 60 bacteroides fragilis, 6 peptococcus. the new cephalosporins showed an increase in the in vitro activity against bacteroides fragilis, but their efficacy was still inferior to that of the 5-nitro-imidazole derivatives. | 1981 | 6259592 |
| role of the intestinal flora in the metabolism of misonidazole. | 1980 | 6260109 | |
| human very low density lipoprotein apolipoprotein e isoprotein polymorphism is explained by genetic variation and posttranslational modification. | 1981 | 6260135 | |
| purification and characterization of clostridium perfringens delta-toxin. | delta-toxin, an extracellular hemolysin released by clostridium perfringens type c, was purified from culture supernatant fluid by sequential ammonium sulfate precipitation, thiol-sepharose gel chromatography, isoelectric focusing, and sephadex g-75 gel filtration. the purified preparation had a specific activity of 320,000 hemolytic units per mg of protein and was homogeneous, as determined by immunochemical and electrophoretic tests. this toxin was characterized as a single polypeptide chain c ... | 1981 | 6260669 |
| common polysaccharide antigens from the cell envelope of clostridium perfringens type a. | soluble antigens were obtained by extracting five serotype strains of clostridium perfringens type a with water at 100 degrees c. the type-specific polysaccharides were precipitated with ethanol, and the common antigens were recovered from the ethanol supernatants by concentration, dialysis, and lyophilization. refluxing the water-extracted cell residues with 1% acetic acid followed by concentration, dialysis, and lyophilization gave additional common antigen fractions. a comprehensive, side-by- ... | 1981 | 6260671 |
| mechanism of nitrite-induced germination of clostridium perfringens spores. | 1980 | 6260726 | |
| [spontaneous peritonitis due to clostridium perfringens in three patients with cirrhosis (author's transl)]. | three cases of spontaneous peritonitis due to clostridium perfringens in cirrhotic patients with a fatal outcome are reported. the diagnosis was made clinically in two patients and by post-mortem examination in the third. one patient had elevated values of serum alpha-fetoprotein. these cases are compared with three other reported cases in the literature. blood cultures were negative in the three patients, a fact that lends support to the theory of transmural migration of bacteria. in the author ... | 1981 | 6261049 |
| considerations on the value of freeze-etching technique in studying the ultrastructure of some anaerobic bacteria. | samples from periodic cultures of clostridium perfringens, clostridium oedematiens, clostridium tetani and bifidobacterium bifidum grown in liquid nutrient media were studied after freeze-fracturing and etching without pretreatment by means of chemical agents including cryo-protectants. subunits in the cell all of cl. tetani and differences in the distribution of globular particles in the plasma membrane of the investigated bacteria are revealed. | 1981 | 6261293 |
| some aspects of microbial contamination of hands of workers in food industries. | with regard to food hygiene, the question is posed whether carriers of pathogenic organisms, like salmonella can contaminate a product by their hands. this might especially be possible in case of bad toilet hygiene. experiments were carried out in 13 food and 3 non-food establishments. an inquiry was made into habits of toilet use and hand washing. the following microbiological examinations were performed on workers' hands: total colony-forming-units per hand, and the presence of salmonella, e. ... | 1981 | 6261483 |
| prevalence of food-poisoning (enterotoxigenic) clostridium perfringens type a in blood and fish meal. | four of 9 strains of cl. perfringens type a isolated from 4 of 7 blood meal and 5 of 12 fish meal samples were found to be enterotoxigenic on biological tests and immunodiffusion test with enterotoxin specific serum. enterotoxin produced by these strains was identical with that of food-poisoning strains. of these 4 strains, 3 were heat-resistant and 1 was heat-sensitive spore formers. rate of sporulation of enterotoxigenic strains was greater than the non-enterotoxigenic ones. animal and public ... | 1981 | 6261484 |
| effects of ph shifts, bile salts, and glucose on sporulation of clostridium perfringens nctc 8798. | the sporulation of clostridium perfringens nctc 8798 was studied after exposing vegetative cells to: ph values of 1.5 to 8.0 in fluid thioglycolate broth (for 2h) and then transferring them to duncan-strong (ds) sporulation medium; sodium cholate or sodium deoxycholate (0.3 to 6.5 mm) in ds medium; or rhia-solberg medium with 0.4% (wt/wt) starch, glucose, or both added at 0 to 55 mm. at ph 1.5, no culturable heat-resistant spores were formed. for cells exposed to ph 3.0, 4.0, 5.0, or 6.0, increa ... | 1981 | 6261681 |
| enterotoxin formation by clostridium perfringens type a in a defined medium. | enterotoxin was produced by 9 of 10 strains of clostridium perfringens type a when grown in a defined medium. additional dextrin increased the amount of enterotoxin in extracts of sporulating cells of strain nctc 10239. | 1981 | 6261685 |
| survival of clostridial spores in animal tissues. | spores injected intravenously into mice in numbers in excess of 10(2)/g of body weight were initially dispersed to most organs, but after a few days the remaining spores were concentrated in the liver, from which they were eliminated with a half-life of about 6 days. intraperitoneal injection did not result in contamination of organs unless initial spore numbers exceeded 10(5)/g of body weight, in which case the spores behaved in the same manner as those injected intravenously. oral administrati ... | 1981 | 6261688 |