Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antibacterial activity of traditional australian medicinal plants. | fifty-six ethanolic extracts of various parts of 39 plants used in traditional australian aboriginal medicine were investigated for their antibacterial activities against four gram-positive (bacillus cereus, enterococcus faecalis, staphylococcus aureus and streptococcus pyogenes) and four gram-negative (escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and salmonella typhimurium) bacterial species. in a plate-hole diffusion assay, 12 extracts inhibited the growth of one or more of ... | 2001 | 11535358 |
| anthrax. | bacillus anthracis was shown to be the etiological agent of anthrax by r. koch and l. pasteur at the end of the nineteenth century. the concepts on which medical microbiology are based arose from their work on this bacterium. the link between plasmids and major virulence factors of b. anthracis was not discovered until the 1980s. the three toxin components are organized in two a-b type toxins, and the bacilli are covered by an antiphagocytic polyglutamic capsule. structure-function analysis of t ... | 2001 | 11544370 |
| nontypical bacillus cereus outbreak. | 2001 | 11544846 | |
| combined effect of nisin and carvacrol at different ph and temperature levels on the viability of different strains of bacillus cereus. | the influence of ph and temperature on the bactericidal action of nisin and carvacrol on vegetative cells of different bacillus cereus strains was studied. the five strains tested showed significant differences in sensitivity towards nisin, at ph 7.0 and 30 degrees c. carvacrol concentrations of 0.3 mmol l(-1) had no effect on viability of b. cereus cells. when the same carvacrol concentration was combined with nisin, however, it resulted in a greater loss of viability of cells than when nisin w ... | 2001 | 11545214 |
| evaluation of microbiological quality of medicinal plants used in natural infusions. | consumption of preparations of medicinal plants has been increasing during the last decades in occidental societies. however, there are no effective sanitary controls of these products. to evaluate the nature and content of microbiological contamination, 62 samples of seven medicinal plants (chamomile, leaves of orange tree, flowers of linden, corn silk, marine alga, pennyroyal mint and garden sage) were studied, using conventional microbiological methods. practically all samples (96.8%) were co ... | 2001 | 11545215 |
| metal ion binding and coordination geometry for wild type and mutants of metallo-beta -lactamase from bacillus cereus 569/h/9 (bcii): a combined thermodynamic, kinetic, and spectroscopic approach. | one high affinity (nm) and one low affinity (microm) macroscopic dissociation constant for the binding of metal ions were found for the wild-type metallo-beta-lactamase from bacillus cereus as well as six single-site mutants in which all ligands in the two metal binding sites were altered. surprisingly, the mutations did not cause a specific alteration of the affinity of metal ions for the sole modified binding site as determined by extended x-ray absorption fine structure (exafs) and perturbed ... | 2001 | 11551939 |
| detection of anthrax spores from the air by real-time pcr. | to detect and isolate bacillus anthracis from the air by a simple and rapid procedure. | 2001 | 11555211 |
| a simple and sensitive detection system for bacillus anthracis in meat and tissue. | to detect and isolate bacillus anthracis from meat and tissue by rapid and simple procedures. | 2001 | 11556906 |
| a simple modified membrane filtration medium for the enumeration of aerobic spore-bearing bacilli in water. | aerobic spore-bearing bacilli have been proposed as a surrogate indicator for the removal of cryptosporidium by drinking water treatment processes. pasteurisation of samples followed by culture on non-selective media is the method of choice. using white membranes for filtration of water samples makes colony counting difficult. vital dyes such as neutral red or trypan blue can help when added to the growth medium but these dyes tend to colour the membrane and reduce the contrast between the colon ... | 2001 | 11561641 |
| thiomandelic acid, a broad spectrum inhibitor of zinc beta-lactamases: kinetic and spectroscopic studies. | resistance to beta-lactam antibiotics mediated by metallo-beta-lactamases is an increasingly worrying clinical problem. candidate inhibitors include mercaptocarboxylic acids, and we report studies of a simple such compound, thiomandelic acid. a series of 35 analogues were synthesized and examined as metallo-beta-lactamase inhibitors. the k(i) values (bacillus cereus enzyme) are 0.09 microm for r-thiomandelic acid and 1.28 microm for the s-isomer. structure-activity relationships show that the th ... | 2001 | 11564740 |
| the cell envelope-bound metalloprotease (camelysin) from bacillus cereus is a possible pathogenic factor. | a novel membrane proteinase of the nosocomial important bacteria species bacillus cereus (synonyms: camelysin, ccmp) was purified up to homogeneity as was shown by mass spectrometry in its amphiphilic form. camelysin is a neutral metalloprotease with a molecular mass of 19 kda. its unique n-terminus phe-phe-ser-asp-lys-glu-val-ser-asn-asn-thr-phe-ala-ala-gly-thr-leu-asp-leu-thr-leu-asn-pro-lys-thr-leu-val-asp-(ile-lys-asp)- was not detected in the protein data bases during blast searches, but in ... | 2001 | 11566257 |
| gern, an endospore germination protein of bacillus cereus, is an na(+)/h(+)-k(+) antiporter. | gern, a bacillus cereus spore germination protein, exhibits homology to a widely distributed group of putative cation transporters or channel proteins. gern complemented the na(+)-sensitive phenotype of an escherichia coli mutant that is deficient in na(+)/h(+) antiport activity (strain knabc). gern also reduced the concentration of k(+) required to support growth of an e. coli mutant deficient in k(+) uptake (strain tk2420). in a fluorescence-based assay of everted e. coli knabc membrane vesicl ... | 2001 | 11566988 |
| fluorescent amplified fragment length polymorphism analysis of norwegian bacillus cereus and bacillus thuringiensis soil isolates. | we examined 154 norwegian b. cereus and b. thuringiensis soil isolates (collected from five different locations), 8 b. cereus and 2 b. thuringiensis reference strains, and 2 bacillus anthracis strains by using fluorescent amplified fragment length polymorphism (aflp). we employed a novel fragment identification approach based on a hierarchical agglomerative clustering routine that identifies fragments in an automated fashion. no method is free of error, and we identified the major sources so tha ... | 2001 | 11571195 |
| isolation of bacillus thuringiensis from intertidal brackish sediments in mangroves. | intertidal brackish sediments in mangroves were examined for isolation of bacillus thuringiensis strains with novel toxicity spectra. a total of 18 b. thuringiensis isolates were recovered from eight sediment samples (36.4%) out of 22 samples tested. the frequency of b. thuringiensis was 1.3% among the colonies of bacillus cereus/b. thuringiensis group. while five isolates were allocated to the four h serogroups, the majority of the isolates were serologically untypable or untestable. two isolat ... | 2001 | 11572461 |
| novel immuno-fret assay method for bacillus spores and escherichia coli o157:h7. | novel immunofluorescence resonance energy transfer (immuno-fret) assays for both bacillus cereus spores and escherichia coli o157:h7 are reported. both assays involve the use of dual (qsy-7 and oregon green 514-antibody)-labeled spores or vegetative bacteria, such that oregon green 514-labeled antibodies are quenched by proximal qsy-7 molecules that are covalently bound to the dual (oregon green 514 and qsy-7)-labeled cells. upon introduction of unlabeled bacteria or spores, in the respective as ... | 2001 | 11573946 |
| challenge testing of the lactoperoxidase system in pasteurized milk. | to determine the role of lactoperoxidase (lp) in inhibiting the growth of micro-organisms in pasteurised milk. | 2001 | 11576311 |
| effects of amino acids on the amidation of polyaromatic carboxylic acids by bacillus cereus. | the soil bacterium bacillus cereus tim-r01 efficiently transformed polyaromatic carboxylic acids (paca) such as 4-biphenylcarboxylic acid (4-bpca), 4-biphenylacetic acid, and 4-phenoxybenzoic acid into their corresponding amides. the amidation activity was expressed at 37 degrees c (ph 7-8) in the presence of grown cells in nutrients under an aerobic atmosphere. other strains of b. cereus, ifo 3001 and iam 1229, also gave the amide from 4-bpca. in phosphate-buffered saline (pbs), the addition of ... | 2001 | 11577715 |
| inexplicable suppression of hepatic uptake of gallium-67, a case report. | we describe here a case report of a patient with acute lymphocytic leukemia in whom hepatic gallium-67 (ga-67) uptake was suppressed. the patient was hospitalized with increasing dyspnea. in ga-67 scintigraphy, there was no hepatic uptake, although other physiological uptake was clearly observed. in addition, the scintigraphy showed increased accumulation in the right lung consistent with infection. we considered possible reasons for these findings. the patient had no history of chemotherapy or ... | 2001 | 11577765 |
| distribution of s-layers on the surface of bacillus cereus strains: phylogenetic origin and ecological pressure. | bacillus anthracis, bacillus cereus and bacillus thuringiensis have been described as members of the bacillus cereus group but are, in fact, one species. b. anthracis is a mammal pathogen, b. thuringiensis an entomopathogen and b. cereus a ubiquitous soil bacterium and an occasional human pathogen. in two clinical isolates of b. cereus, in some b. thuringiensis strains and in b. anthracis, an s-layer has been described. we investigated how the s-layer is distributed in b. cereus, and whether phy ... | 2001 | 11578310 |
| bacillus cereus keratitis associated with contact lens wear. | we report the first case of contact lens-related bacillus cereus keratitis and ulcer associated with b. cereus contamination of the contact lens case. this is also the first study to investigate and establish the genetic identity of an organism isolated from the cornea and contact lens case in a patient with contact lens-associated keratitis. | 2001 | 11581057 |
| a catalytic diad involved in substrate-assisted catalysis: nmr study of hydrogen bonding and dynamics at the active site of phosphatidylinositol-specific phospholipase c. | phosphatidylinositol-specific phospholipase cs (pi-plcs, ec 3.1.4.10) are ubiquitous enzymes that cleave phosphatidylinositol or phosphorylated derivatives, generating second messengers in eukaryotic cells. a catalytic diad at the active site of bacillus cereus pi-plc composed of aspartate-274 and histidine-32 was postulated from the crystal structure to form a catalytic triad with the 2-oh group of the substrate [heinz, d. w., et al. (1995) embo j. 14, 3855-3863]. this catalytic diad has been o ... | 2001 | 11583175 |
| molecular dynamics simulations of the mononuclear zinc-beta-lactamase from bacillus cereus complexed with benzylpenicillin and a quantum chemical study of the reaction mechanism. | herein, we present results from md simulations of the michaelis complex formed between the b. cereus zinc-beta-lactamase enzyme and benzylpenicillin. the structural and dynamical effects induced by substrate-binding, the specific role of the conserved residues, and the near attack conformers of the michaelis complex are discussed. quantum chemical methods (hf/6-31g* and b3lyp/6-31g*) are also applied to study the hydrolysis reaction of n-methylazetidinone catalyzed by a monozinc system consistin ... | 2001 | 11583551 |
| molecular characterization of a phosphatidylcholine-hydrolyzing phospholipase c. | while searching for a phospholipase c (plc) specific for phosphatidylcholine in mammalian tissues, we came across such an activity originating from a contamination of pseudomonas fluorescens. this psychrophilic bacterium was found to contaminate placental extracts upon processing in the cold. the secreted phosphatidylcholine-hydrolyzing plc was purified by a combination of chromatographic procedures. as substrates, the enzyme preferred dipalmitoyl-phosphatidylcholine and 1-palmitoyl-2-arachidono ... | 2001 | 11589699 |
| mode of action of beta-barrel pore-forming toxins of the staphylococcal alpha-hemolysin family. | staphylococcal alpha-hemolysin is the prototype of a family of bacterial exotoxins with membrane-damaging function, which share sequence and structure homology. these toxins are secreted in a soluble form which finally converts into a transmembrane pore by assembling an oligomeric beta-barrel, with hydrophobic residues facing the lipids and hydrophilic residues facing the lumen of the channel. besides alpha-hemolysin the family includes other single chain toxins forming homo-oligomers, e.g. beta ... | 2001 | 11595629 |
| effect of bacillus cereus exocellular factors on human intestinal epithelial cells. | to gain insight on the biological effects of the exocellular factors produced by bacillus cereus, culture filtrate supernatants of different strains were coincubated with differentiated caco-2 cells. exocellular factors were able to detach enterocyte-like cells from the substratum after 1 h of incubation. in addition, microvilli effacing and dramatic changes on the cellular surface of enterocytes were found after incubation periods as short as 20 min. since cell detachment was not inhibited by f ... | 2001 | 11601702 |
| inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power. | antimicrobial activity of honey has been attributed to hydrogen peroxide, which is produced by naturally occurring glucose oxidase, and phenolic compounds, although lethality of and inhibition by these and other components against microorganisms vary greatly, depending on the floral source of nectar. this study was undertaken to compare honeys from six floral sources for their inhibitory activity against escherichia coli o157:h7, salmonella typhimurium, shigella sonnei, listeria monocytogenes, s ... | 2001 | 11603859 |
| growth and toxin profiles of bacillus cereus isolated from different food sources. | eleven strains of bacillus cereus isolated from milk and meat products have been used to study growth and sporulation profiles in detail. polymerase chain reaction (pcr) using primers detecting cold shock protein a gene signatures (cspa), showed that none of the strains were the newly suggested species in the b. cereus group, b. weihenstephanensis, comprising psychrotolerant cereus strains, although one of the strains grew at 4 degrees c, two at 6 degrees c and seven grew at 7 degrees c. one of ... | 2001 | 11603861 |
| dynamics of mononuclear cadmium beta-lactamase revealed by the combination of nmr and pac spectroscopy. | the two metal sites in cadmium substituted beta-lactamase from bacillus cereus 569/h/9 have been studied by nmr spectroscopy ((1)h, (15)n, and (113)cd) and pac spectroscopy ((111m)cd). distinct nmr signals from the backbone amides are identified for the apoenzyme and the mononuclear and binuclear cadmium enzymes. for the binuclear cadmium enzyme, two (113)cd nmr signals (142 and 262 ppm) and two (111m)cd pac nuclear quadrupole interactions are observed. two nuclear quadrupole interactions are al ... | 2001 | 11603983 |
| synthesis and kinetic evaluation of inhibitors of the phosphatidylinositol-specific phospholipase c from bacillus cereus. | substrate analogues of phosphatidylinositol (1) were synthesized and evaluated as potential inhibitors of the bacterial phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus. the chiral analogues of the water-soluble phospholipid substrate 5 were designed to probe the effects of varying the inositol c-2 hydroxyl group, which is generally believed to serve as the nucleophile in the first step of the hydrolysis of phosphatidylinositols by pi-plc. in the analogues 6-9, the c-2 ... | 1996 | 11667784 |
| identification of catalytic and substrate-binding site residues in bacillus cereus atcc7064 oligo-1,6-glucosidase. | three active site residues (asp199, glu255, asp329) and two substrate-binding site residues (his103, his328) of oligo-1,6-glucosidase (ec 3.2.1.10) from bacillus cereus atcc7064 were identified by site-directed mutagenesis. these residues were deduced from the x-ray crystallographic analysis and the comparison of the primary structure of the oligo-1,6-glucosidase with those of saccharomyces carlsbergensis alpha-glucosidase, aspergillus oryzae alpha-amylase and pig pancreatic alpha-amylase which ... | 2001 | 11676021 |
| activation of the jak/stat pathway by ceramide in cultured human fibroblasts. | endogenous ceramide (cer) was generated by treatment of cultured fibroblasts with sphingomyelinase (smase) from bacillus cereus. a 30 min treatment with 0.1-0.3 u/ml smase induced a dose-dependent increase in the intracellular level of cer. the activation of the transcription factors signal transducer and activator of transcription (stat) 1 and stat3 by smase was investigated by determination of the phosphorylation state by immunoblot, and of dna binding activity by electrophoretic mobility shif ... | 2001 | 11684091 |
| [effect of introduced microorganisms on copper and cobalt mobility in soil]. | the paper deals with the change of copper and cobalt mobility in contaminated soil when activating microbiological processes by introduction of sources of carbon, nitrogen, phosphorus, metal-resistant microorganisms. when bacillus cereus bkm 4368 growing in soil fertilized with glucose one can observe a 5-fold increase of cocentration of heavy-metal mobile forms as compared with sterile soil. the use of acetate as a carbon source and alcaligenes euthrophus ch 34 culture as inoculum will provide ... | 2001 | 11692680 |
| evaluation of a spray-dried lacticin 3147 powder for the control of listeria monocytogenes and bacillus cereus in a range of food systems. | the potential of a powdered preparation of the bacteriocin, lacticin 3147, was investigated for the inhibition of listeria monocytogenes and bacillus cereus. | 2001 | 11696102 |
| crystal structure of the anthrax lethal factor. | lethal factor (lf) is a protein (relative molecular mass 90,000) that is critical in the pathogenesis of anthrax. it is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (mapkk) family near to their amino termini, leading to the inhibition of one or more signalling pathways. here we describe the crystal structure of lf and its complex with the n terminus of mapkk-2. lf comprises four domains: domain i binds the membrane-translocating component of anth ... | 2001 | 11700563 |
| evaluation of the bact/alert automated blood culture system for detecting bacteria and measuring their growth kinetics in leucodepleted and non-leucodepleted platelet concentrates. | to evaluate the bact/alert automated blood culture system for the detection of bacteria in platelet concentrates, and to determine bacterial growth kinetics in leucodepleted and non-leucodepleted units. | 2001 | 11703857 |
| cloning and sequencing of two genes encoding chitinases a and b from bacillus cereus ch. | two genes encoding chitinases a and b (chia and chib) from bacillus cereus ch were cloned into escherichia coli xl1-blue mrf', by using pbluescript ii sk+, and their nucleotide sequences were determined. open reading frames of the chia and chib genes encoded distinct polypeptide chains consisting of 360 and 674 amino acid residues, respectively, with calculated molecular sizes of 39,470 and 74,261 da, respectively. comparison of the deduced amino acid sequences with those of other bacterial chit ... | 2001 | 11718542 |
| optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approach. | the utility of a high-density oligonucleotide microarray (microchip) for identifying strains of five closely related bacilli (bacillus anthracis, bacillus cereus, bacillus mycoides, bacillus medusa and bacillus subtilis) was demonstrated using an approach that compares the non-equilibrium dissociation rates ('melting curves') of all probe-target duplexes simultaneously. for this study, a hierarchical set of 30 oligonucleotide probes targeting the 16s ribosomal rna of these bacilli at multiple le ... | 2001 | 11722542 |
| characterization of spore appendages from bacillus cereus strains. | further characterization and comparison of spore appendages from bacillus cereus strains. | 2001 | 11722661 |
| nanoscale powders and formulations with biocidal activity toward spores and vegetative cells of bacillus species, viruses, and toxins. | certain formulations of nanoscale powders possess antimicrobial properties. these formulations are made of simple, nontoxic metal oxides such as magnesium oxide (mgo) and calcium oxide (cao, lime) in nanocrystalline form, carrying active forms of halogens, for example, mgo. cl2 and mgo. br2. when these ultrafine powders contact vegetative cells of escherichia coli, bacillus cereus, or bacillus globigii, over 90% are killed within a few minutes. likewise, spore forms of the bacillus species are d ... | 2002 | 11727041 |
| cobalt(ii) and copper(ii) binding of bacillus cereus trinuclear phospholipase c: a novel 1h nmr spectrum of a 'tri-cu(ii)' center in protein. | the phosphatidylcholine-preferring phospholipase c from bacillus cereus (pc-plc(bc)) is a tri-zn enzyme with two 'tight binding' and one 'loose binding' sites. the zn2+ ions can be replaced with co2+ and cu2+ to afford metal-substituted derivatives. two cu2+-substituted derivatives are detected by means of 1h nmr spectroscopy, a 'transient' derivative and a 'stable' derivative. the detection of sharp hyperfine-shifted 1h nmr signals in the 'transient' derivative indicates the formation of a magn ... | 2001 | 11730896 |
| bacillus anthracis pxo1 plasmid sequence conservation among closely related bacterial species. | the complete sequencing and annotation of the 181.7-kb bacillus anthracis virulence plasmid pxo1 predicted 143 genes but could only assign putative functions to 45. hybridization assays, pcr amplification, and dna sequencing were used to determine whether pxo1 open reading frame (orf) sequences were present in other bacilli and more distantly related bacterial genera. eighteen bacillus species isolates and four other bacterial species were tested for the presence of 106 pxo1 orfs. three orfs wer ... | 2002 | 11741853 |
| isolation and expression of a bacillus cereus gene encoding benzil reductase. | benzil was reduced stereospecifically to (s)-benzoin by bacillus cereus strain tim-r01. to isolate the gene responsible for asymmetric reduction, we constructed a library consisting of escherichia coli clones that harbored plasmids expressing bacillus cereus genes. the library was screened using the halo formation assay, and one clone showed benzil reduction to (s)-benzoin. thus, this clone seemed to carry a plasmid encoding a bacillus cereus benzil reductase. the deduced amino acid sequence had ... | 2001 | 11745140 |
| detection of specific bacillus anthracis spore biomarkers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms) was applied for the characterization of bacillus anthracis spore biomarkers. b. anthracis spores were extracted under a simple procedure, followed by linear mode analysis, using sinapinic acid as the matrix. several markers with a mass range of 4-7 kda were detected in three b. anthracis strains: vollum, sterne and v770-np1-r. similar spectra were also obtained for spore extracts of two members ... | 2001 | 11746875 |
| the antibacterial activity of triclosan-impregnated storage boxes against staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, bacillus cereus and shewanella putrefaciens in conditions simulating domestic use. | antimicrobial resistance has increased over the past decade causing concern for public health. domestic antimicrobial products containing triclosan (2,4,4'-trichloro-2'-hydroxydiphenylether), a broad-spectrum antibacterial agent, were introduced in 1997 and have become popular among consumers. cross-resistance to other antibacterial agents has been suggested as a possible consequence of their widespread use. triclosan-impregnated plastic storage boxes were tested for activity against staphylococ ... | 2002 | 11751771 |
| molecular cloning and expression of mn(2+)-dependent sphingomyelinase/hemolysin of an aquatic bacterium, pseudomonas sp. strain tk4. | we report here the molecular cloning and expression of a hemolytic sphingomyelinase from an aquatic bacterium, pseudomonas sp. strain tk4. the sphingomyelinase gene was found to consist of 1,548 nucleotides encoding 516 amino acid residues. the recombinant 57.7-kda enzyme hydrolyzed sphingomyelin but not phosphatidylcholine, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, or phosphatidylethanolamine, indicating that the enzyme is a sphingomyelin-specific sphingomyelinase c. the hydr ... | 2002 | 11751833 |
| c3stau, a new member of the family of c3-like adp-ribosyltransferases. | c3-like adp-ribosyltransferases, which are produced by clostridium botulinum, clostridium limosum, bacillus cereus and staphylococcus aureus, are exoenzymes lacking a translocation unit. these enzymes specifically inactivate rho gtpases in host target cells. recently, a novel c3-like transferase from s. aureus with new properties was identified, raising questions regarding its function. as rho gtpases are master regulators of several eukaryotic signal processes and s. aureus can invade eukaryoti ... | 2002 | 11755073 |
| synthesis and biological activities of indolocarbazoles bearing amino acid residues. | three indolocarbazole compounds bearing a tripeptide or a lysine group attached to one of the indole nitrogens via a propylamino chain and two rebeccamycin derivatives bearing a lysine residue on the sugar moiety were synthesised with the aim of improving the binding to dna and the antiproliferative activities. four tumour cell lines, from murine l1210 leukemia, human ht29 colon carcinoma, a549 non-small cell lung carcinoma and k-562 leukemia, were used to evaluate the cytotoxicity of the drugs. ... | 2001 | 11755231 |
| arthromitus (bacillus cereus) symbionts in the cockroach blaberus giganteus: dietary influences on bacterial development and population density. | the filamentous spore-forming bacterium arthromitus, discovered in termites, millipedes, sow bugs and other soil-dwelling arthropods by leidy (1850), is the intestinal stage of bacillus cereus. we extend the range of arthromitus habitats to include the hindgut of blaberus giganteus, the large tropical american cockroach. the occurrence and morphology of the intestinal form of the bacillus were compared in individual cockroaches (n=24) placed on four different diet regimes: diurnally maintained i ... | 1999 | 11762374 |
| [synthesis of anabiosis autoinducers in non-spore-forming bacteria as a mechanism regulating their activity in soil and subsoil sedimentary rocks]. | non-spore-forming bacteria of the genera arthrobacter and micrococcus, isolated from permafrost subsoil, were found to produce greater amounts of the d1 extracellular factor than closely related collection strains isolated from soil. the effect of this factor, responsible for cell transition to anabiosis, was not species-specific. thus, the d1 crude preparation isolated from the culture liquid of the permafrost isolate arthrobacter globiformis 245 produced an effect on the collection strain arth ... | 2001 | 11763780 |
| prediction of microbial growth in fresh-cut vegetables treated with acidic electrolyzed water during storage under various temperature conditions. | effects of storage temperature (1, 5, and 10 degrees c) on growth of microbial populations (total aerobic bacteria, coliform bacteria, bacillus cereus, and psychrotrophic bacteria) on acidic electrolyzed water (acew)-treated fresh-cut lettuce and cabbage were determined. a modified gompertz function was used to describe the kinetics of microbial growth. growth data were analyzed using regression analysis to generate "best-fit" modified gompertz equations, which were subsequently used to calculat ... | 2001 | 11770620 |
| tandem mass spectrometry of intact proteins for characterization of biomarkers from bacillus cereus t spores. | intact protein biomarkers from bacillus cereus t spores have been analyzed by high-resolution tandem fourier transform ion cyclotron resonance mass spectrometry. two techniques have been applied for excitation of the isolated multiply charged precursor ion species: sustained off-resonance irradiation/collisionally activated dissociation and electron capture dissociation. fragmentation-derived sequence tags and blast sequence similarity proteome database searches allow unequivocal identification ... | 2001 | 11774913 |
| [characteristics of bacillus cereus dissociants]. | the autoregulation of the phenotypic (populational) variability of the bacillus cereus strain 504 was studied. the isolated colonial morphotypes of this bacterium were found to differ in their growth characteristics and the synthesis of extracellular proteases. the phenotypic variabilities of vegetative proliferating cells and those germinated from endospores and cystlike refractory cells were different. bacterial variants also differed in the production of the d1 and d2 factors (the autoinducer ... | 2001 | 11785138 |
| the enzymes with benzil reductase activity conserved from bacteria to mammals. | the diketone compound, benzil is reduced to (s)-benzoin with living bacillus cereus cells. recently, we isolated a gene responsible for benzil reduction, and escherichia coli cells in which this gene was overexpressed transformed benzil to (s)-benzoin. although this benzil reductase showed high identity to the short-chain dehydrogenase/reductase (sdr) family, enzymological features were unknown. here, we demonstrated that many b. cereus strains had benzil reductase activity in vivo, and that the ... | 2002 | 11796169 |
| molecular analysis of rifampin resistance in bacillus anthracis and bacillus cereus. | rifampin-resistant mutants were selected from uv-light-treated bacillus cereus (20 mutants) and attenuated b. anthracis (23 mutants). in addition, spontaneous rifampin-resistant mutants were also isolated in attenuated b. anthracis (22 mutants). the rifampin resistance clusters of the rpob gene were sequenced for all 65 mutants. mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. the spontaneous rate of resistance was estim ... | 2002 | 11796364 |
| membrane restructuring via ceramide results in enhanced solute efflux. | the capacity of ceramides to modify the permeability barrier of cell membranes has been explored. membrane efflux induced either by in situ generated ceramides (through enzymatic cleavage of sphingomyelin) or by addition of ceramides to preformed membranes has been studied. large unilamellar vesicles composed of different phospholipids and cholesterol, and containing entrapped fluorescent molecules, have been used as a system to assay ceramide-dependent efflux. small proportions of ceramide (10 ... | 2002 | 11796726 |
| effects of antibacterial dishwashing liquid on foodborne pathogens and competitive microorganisms in kitchen sponges. | in response to increasing concern about home hygiene, the use of antibacterial products to reduce microorganisms in kitchen sponges and cleaning cloths is strongly promoted by some producers of detergent for domestic use. the effects of an antibacterial dishwashing liquid on escherichia coli, salmonella enteritidis, staphylococcus aureus, and bacillus cereus were investigated in a modified suspension test and in used sponges with and without food residues under laboratory conditions. a limited s ... | 2002 | 11811157 |
| epidemiologic profiling: evaluating foodborne outbreaks for which no pathogen was isolated by routine laboratory testing: united states, 1982-9. | the objective was to evaluate foodborne outbreaks of undetermined aetiology by comparing them to pathogen-specific epidemiologic profiles of laboratory-confirmed foodborne outbreaks. national foodborne outbreak data reported to cdc during 1982-9 were categorized by clinico-epidemiologic profiles based on incubation, duration, percent vomiting, fever and vomiting to fever ratio. from the pathogen-specific profiles, five syndromes were developed: a vomiting-toxin syndrome resembling bacillus cereu ... | 2001 | 11811869 |
| substrate binding and catalytic mechanism of class b beta-lactamases: a molecular modelling study. | increased resistance to beta-lactam antibiotics is mainly due to beta-lactamases whose production by pathogenic bacteria makes their broad activity spectrum especially frightening. x-ray structures of several zinc beta-lactamases have revealed the coordination of the two metal ions, but their mode of action remains unclear. geometry optimisation of stable complexes along the reaction pathway of benzylpenicillin hydrolysis highlighted a proton shuttle occurring from d120 of the bacillus cereus be ... | 2001 | 11814063 |
| exploring the role and the binding affinity of a second zinc equivalent in b. cereus metallo-beta-lactamase. | metallo-beta-lactamases are a newly characterized family of zinc enzymes present in several pathogenic strains that represent an emerging clinical threat. enzymes from different organisms exhibit an outstanding functional diversity, particularly in the metal ion requirements for activity. we have investigated the effect of the second zinc(ii) equivalent in the enzyme betalii from bacillus cereus, naturally active in the mono-zinc(ii) form. the enzyme is reversibly inactivated at low ph, due to d ... | 2002 | 11827530 |
| selection of metalloenzymes by catalytic activity using phage display and catalytic elution. | the metallo-beta-lactamase betalii from bacillus cereus 569/h/9 was displayed on the filamentous phage fd. the phage-bound enzyme fd-betalii was shown to be active on benzylpenicillin as substrate; it could be inactivated by complexation of the essential zinc(ii) ion with edta and reactivated by addition of a zinc(ii) salt. a selection process was designed to extract active phage-bound enzymes from libraries of mutants in three steps: 1. inactivation of active phage-bound enzymes by metal ion co ... | 2001 | 11828452 |
| selective enrichment and characterization of a phosphorus-removing bacterial consortium from activated sludge. | under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. the phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. the addition of calcium and magnesium salts, as well as vitamins and trace elements, to autoclaved sterile filtrate of activated sludge was not sufficient to achieve stable phosphorus removal ... | 2002 | 11831470 |
| selection of bacteriocin producer strains of lactic acid bacteria from a dairy environment. | two strains showing bacteriocin production were selected from a total of 206 lactic acid bacteria isolated from samples of milk, milk serum, whey and homemade cheeses in southern cordoba, argentina. this property was detected by means of well diffusion assays. the strains were identified as enterococcus hirae and enterococcus durans. the protein nature of those substances was proved by showing their sensitivity to type iv and xxv proteases, papaine, trypsin, pepsin and k proteinase. the bacterio ... | 2002 | 11837389 |
| bacteriocin-producing lactobacillus sake as starter culture in dry sausages. | one hundred and fifty-two strains of lactobacillus spp and micrococcus spp, isolated from dry sausages, were screened for inhibitory activity. two of the strains assayed of the genus lactobacillus showed bactericidal activity. they were able to inhibit listeria monocytogenes, listeria seeligeri, listeria innocua, lactobacillus alimentarius and lactobacillus bavaricus. the strains of escherichia coli, salmonella bradford and salmonella newlands, staphylococcus aureus, bacillus cereus, pseudomonas ... | 2002 | 11837390 |
| fish chromatophores as cytosensors in a microscale device: detection of environmental toxins and bacterial pathogens. | fish chromatophores from betta splendens are used as the cytosensor element in the development of a portable microscale device capable of detecting certain environmental toxins and bacterial pathogens by monitoring changes in pigment granule distribution. the adaptation of chromatophores to a microscale environment has required the development of enabling technologies to produce miniaturized culture chambers, to integrate microfluidics for sample delivery, to miniaturize image capture, and to de ... | 2002 | 11841070 |
| sphingomyelinase cleavage of sphingomyelin in pure and mixed lipid membranes. influence of the physical state of the sphingolipid. | sphingomyelin hydrolysis by sphingomyelinase is essential in regulating membrane levels of ceramide, a well-known metabolic signal. since natural sphingomyelins have a gel-to-fluid transition temperature in the range of the physiological temperatures of mammals and birds, it is important to understand the influence of the physical state of the lipid on the enzyme activity. with that aim, large unilamellar vesicles consisting of pure egg sphingomyelin (gel-to-fluid crystalline transition temperat ... | 2002 | 11841822 |
| on calculating sterility in thermal preservation methods: application of the weibull frequency distribution model. | a simple and parsimonious model which originated from the weibull frequency distribution was proposed to describe nonlinear survival curves of spores. this model was suitable for downward concavity curves (bacillus cereus and bacillus pumilus), as well as for upward concavity curves (clostridium botulinum). it was shown that traditional f values calculated from this new model were no longer additive, to such an extent that a heat treatment should be better characterized by the obtained decimal r ... | 2002 | 11843401 |
| high-pressure inactivation of bacillus cereus spores in the presence of argon. | we investigated the high-pressure inactivation of bacillus cereus spores in water containing argon. at the pressure of 600 mpa, addition of argon accelerated the inactivation of spores at 20 degrees c, but had no effect on the inactivation at 40 degrees c. the influence of added argon on inactivation of the spores was marked under conditions with a strong 'water ordering' effect. the pressure resistance of b. cereus spores was thus shown to be affected by 'water ordering'. | 2002 | 11845823 |
| interaction of the rho-adp-ribosylating c3 exoenzyme with rala. | rhoa, -b, and -c are adp-ribosylated and biologically inactivated by clostridium botulinum c3 exoenzyme and related c3-like transferases. we report that rala gtpase, which is not adp-ribosylated by c3, inhibits adp-ribosylation of rhoa by c3 from c. botulinum (c3bot), clostridium limosum (c3lim), and bacillus cereus (c3cer) but not from staphylococcus aureus (c3stau) in human platelet membranes and rat brain lysate. inhibition by rala occurs with the gdp- and guanosine 5'-3-o-(thio)triphosphate- ... | 2002 | 11847234 |
| functional control of the binuclear metal site in the metallo-beta-lactamase-like fold by subtle amino acid replacements. | at present there are three protein families that share a common structural domain, the alphabeta/betaalpha fold of class b beta-lactamases: zinc beta-lactamases, glyoxalases ii, and a-type flavoproteins. a detailed inspection of their superimposed structures was undertaken and showed that although these proteins contain binuclear metal sites in spatially equivalent positions, there are some subtle differences within the first ligand sphere that determine a distinct composition of metals. althoug ... | 2002 | 11847294 |
| effect of nitrogen gas packaging on the quality and microbial growth of fresh-cut vegetables under low temperatures. | nitrogen (n2) gas packaging for fresh-cut vegetables (lettuce and cabbage) has been examined as a means of modified atmosphere packaging (map) for extending the shelf life of cut vegetables. gas composition in enclosed packages that contained cut vegetables and were filled with 100% n2 had an oxygen (o2) concentration of 1.2 to 5.0% and a carbon dioxide (co2) concentration of 0.5 to 3.5% after 5 days of storage. an atmosphere of low concentrations of o2 and high co2 conditions occurred naturally ... | 2002 | 11848563 |
| the influence of nisin on the thermal resistance of bacillus cereus. | decimal reduction times (d-values) at cooking and autoclaving temperatures (80 to 120 degrees c) of spores of bacillus cereus atcc 1479-8 in rice and milk (13% wt/vol) supplemented with nisin (25 microg/ml) were evaluated. the mean d-values at 97.8 degrees c in cooked white rice, phosphate buffer (ph 7.0), and rice water (ph 6.7) were 3.62, 1,99, and 1.34 min, respectively. from 80 to 100 degrees c, the mean reduction in d-values due to the addition of nisin to milk was 40%. the d-value at 110 d ... | 2002 | 11848577 |
| the effect of nisin on growth kinetics from activated bacillus cereus spores in cooked rice and in milk. | the growth kinetics of germinated cells from activated spores of bacillus cereus in cooked white rice and in milk were evaluated at different temperatures for control samples and for samples with 25 microg of nisin per ml added. nisin was applied in the form of nisaplin (10(6) iu/g), which contained 25,000 microg of nisin per g. the length of the lag phase for cooked white rice controls was 120 h at 10 degrees c, 8 h at 25 degrees c, and 2.5 h at 33 degrees c. the generation times for cooked ric ... | 2002 | 11848578 |
| adaptation of a neutrophilic dairy-associated bacillus cereus isolate to alkaline ph. | this study identified and studied the response of five bacillus strains, isolated from alkaline cleaning in place (cip) solutions, to alkaline conditions. | 2002 | 11849331 |
| differential efficacy of a chlorine dioxide-containing sanitizer against single species and binary biofilms of a dairy-associated bacillus cereus and a pseudomonas fluorescens isolate. | daily exposure to 100 p.p.m. chlorine dioxide of single species and binary biofilms of dairy-associated bacillus cereus dl5 and pseudomonas fluorescens m2, attached to stainless steel surfaces in a laboratory flow system, was studied. | 2002 | 11849365 |
| growth behaviour of a spore forming probiotic strain in the gastrointestinal tract of broiler chicken and piglets. | the growth behaviour of the probiotic strain bacillus cereus var. toyoi in the gastrointestinal tract of broiler chicken and suckling piglets was evaluated. the strain germinated rapidly in intestinal samples from both animal species. less than 10% of spores were recovered from the chicken crop and piglet stomach, respectively. lumen samples and mucosal tissues from the hind gut of piglets displayed increasing colonization of the probiotic strain throughout the trial period. after oral administr ... | 2001 | 11851013 |
| occurrence of bacillus sporothermodurans and other aerobic spore-forming species in feed concentrate for dairy cattle. | to determine the aerobic spore composition and presence of bacillus sporothermodurans spores in feed concentrate for dairy cattle. | 2001 | 11851816 |
| heat and salt stress in the food pathogen bacillus cereus. | the effects of stresses imposed on bacterial contaminants during food processing and treatment of packaging material were evaluated on the food pathogen bacillus cereus. | 2001 | 11851817 |
| effect of carbon dioxide on the growth of bacillus cereus spores in milk during storage. | the effects of the addition of 11.9 mm co2 on the growth of bacillus cereus spores inoculated into sterile homogenized whole milk at 101 and 106 spores/ml and stored at 6.1 degrees c, was examined weekly for 35 d. colony-forming units from co2 treated inoculated milks decreased over 35 d at a rate similar to that of untreated inoculated milk, as defined by linear regression. plate counts for treated and control milks inoculated at 10(1) cfu/ml were not significantly different on sampling d 0, 14 ... | 2002 | 11860107 |
| a mouse serine protease tesp5 is selectively included into lipid rafts of sperm membrane presumably as a glycosylphosphatidylinositol-anchored protein. | we have previously indicated that at least in mouse, sperm serine protease(s) other than acrosin probably act on the limited proteolysis of egg zona pellucida to create a penetration pathway for motile sperm, although the participation of acrosin cannot be ruled out completely. a 42-kda gelatin-hydrolyzing serine protease present in mouse sperm is a candidate enzyme involved in the sperm penetration of the zona pellucida. in this study, we have pcr-amplified an est clone encoding a testicular se ... | 2002 | 11861648 |
| cloning and overexpression of bacillus cereus penicillin-binding protein 3 gene in escherichia coli. | the pbp3 gene encoding pbp3 of bacillus cereus was cloned and sequenced. for this purpose, pbp3 was first purified from b. cereus ts-4, and n-terminal amino acid sequences of the peptides obtained from the protease digests of the protein were analyzed. the b. cereus ts-4 pbp3 gene consisted of an open reading frame of 1,986 bp encoding 662 amino acid residues with a calculated molecular mass of 73,044 da. the active site-motifs sxxk, sxn, and ktg are present at the positions 393, 452, and 590, r ... | 2002 | 11866118 |
| enumeration of total bacteria and bacteria with genes for proteolytic activity in pure cultures and in environmental samples by quantitative pcr mediated amplification. | real-time quantitative pcr assays were developed for the absolute quantification of different groups of bacteria in pure cultures and in environmental samples. 16s rrna genes were used as markers for eubacteria, and genes for extracellular peptidases were used as markers for potentially proteolytic bacteria. for the designed 16s rdna taqman assay, specificity of the designed primer-probe combination for eubacteria, a high amplification efficiency over a wide range of starting copy numbers and a ... | 2002 | 11869788 |
| bacillus cereus cellulitis from contaminated heroin. | concern exists over recent unexplained deaths among intravenous drug users. this report describes a patient with crepitant cellulitis who was admitted complaining of severe pain in the right forearm. ultrasonography demonstrated gas in the tissues and he was referred for early surgical debridement of the arm. he was treated with intravenous benzyl penicillin, gentamicin and metronidazole and made a full recovery. aspirate samples grew bacillus cereus, morphologically similar to the isolate obtai ... | 2002 | 11871624 |
| acid stress in the food pathogen bacillus cereus. | the pathogen bacillus cereus, which is associated with a number of foods including dairy products, was studied for its response to acid stress during the exponential phase. | 2002 | 11872115 |
| genetic differentiation between sympatric populations of bacillus cereus and bacillus thuringiensis. | little is known about genetic exchanges in natural populations of bacteria of the spore-forming bacillus cereus group, because no population genetics studies have been performed with local sympatric populations. we isolated strains of bacillus thuringiensis and b. cereus from small samples of soil collected at the same time from two separate geographical sites, one within the forest and the other at the edge of the forest. a total of 100 b. cereus and 98 b. thuringiensis strains were isolated an ... | 2002 | 11872495 |
| inhibition of bacterial growth, enterotoxin production, and spore outgrowth in strains of bacillus cereus by bacteriocin as-48. | bacteriocin as-48 showed high bactericidal activity for mesophilic and psychrotrophic strains of bacillus cereus over a broad ph range. as-48 inhibition of the enterotoxin-producing strain lwl1 was enhanced by sodium nitrite, sodium lactate, and sodium chloride. the latter also enhanced as-48 activity against strain cect 131. bacterial growth and enterotoxin production by strain lwl1 were completely inhibited at bacteriocin concentrations of 7.5 microg/ml. at subinhibitory bacteriocin concentrat ... | 2002 | 11872505 |
| modified blasticidin s resistance gene (bsrm) as a selectable marker for construction of retroviral vectors. | retroviral vectors are commonly used in ex vivo gene therapy protocols. the structure of vectors basically consists of one gene of interest and a selectable marker gene. fast selection without damaging cells is a critical step for ex vivo gene therapy protocols. blasticidin s deaminase isolated from bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin s (bs). a commercially available gene coding for blasticidin s deaminase (bsr) when used to construct retroviral v ... | 2002 | 11879712 |
| production of bacillus cereus emetic toxin (cereulide) in various foods. | to determine the role of bacillus cereus as a potential pathogen in food poisoning, the production of an emetic toxin (cereulide) by b. cereus was quantified in various food sources. the amount of emetic toxin in 13 of 14 food samples implicated in vomiting-type food poisoning cases ranged from 0.01 to 1.28 microg/g. a vomiting-type strain, b. cereus nc7401, was inoculated into various foods and incubated for 24 h at 20, 30, and 35 degrees c. in boiled rice, b. cereus rapidly increased to 10(7)- ... | 2002 | 11883672 |
| the incompatibility between the plcr- and atxa-controlled regulons may have selected a nonsense mutation in bacillus anthracis. | bacillus anthracis, bacillus thuringiensis and bacillus cereus are members of the bacillus cereus group. these bacteria express virulence in diverse ways in mammals and insects. the pathogenic properties of b. cereus and b. thuringiensis in mammals results largely from the secretion of non-specific toxins, including haemolysins, the production of which depends upon a pleiotropic activator plcr. in b. anthracis, plcr is inactive because of a nonsense mutation in the plcr gene. this suggests that ... | 2001 | 11886551 |
| genetic relationships of bacillus anthracis and closely related species based on variable-number tandem repeat analysis and box-pcr genomic fingerprinting. | variable-number tandem repeats (vntr) analysis and box-repeat-based pcr (box-pcr) genomic fingerprinting were performed on 25 bacillus strains to investigate the genetic relatedness of bacillus anthracis to the closely related species. based on vntr analysis, all b. anthracis strains could be assigned to (vntr)(4), which is the most commonly found type in the world. interestingly, a (vntr)(2) was also observed in bacillus cereus kctc 1661 and with an exact match to the tandem repeats found in b. ... | 2002 | 11886745 |
| gas sensor arrays for early detection of infection in mammalian cell culture. | the detection of bacterial infections in a mammalian cell culture process is realised using a gas sensor array. in production-scale and laboratory-scale cultivations of a perfused recombinant cho-cell culture producing human blood coagulation factor viii, we show that the gas sensor array identifies bacterial contamination earlier than conventional methods. the sensitivity of the instrument is verified by inoculation of a blank cell culture medium with defined bacterial cell counts. | 2002 | 11888730 |
| antimicrobial synergistic effect of linolenic acid and monoglyceride against bacillus cereus and staphylococcus aureus. | the antimicrobial effect of linolenic acid with or without monoglyceride (glycerol laurate or glycerol myristate) against six food-borne microorganisms was determined in broth medium. minimum inhibitory concentration of linolenic acid on bacillus cereus and staphylococcus aureus was 20 and 50 ppm, respectively. the growth of b. cereus treated with linolenic acid at 10 ppm with 10 ppm monoglyceride was more inhibitory than that of linolenic acid alone, and the viable cell population was reduced 2 ... | 2002 | 11902977 |
| influence of the probiotic strain bacillus cereus var. toyoi on the development of enterobacterial growth and on selected parameters of bacterial metabolism in digesta samples of piglets. | the effect of the probiotic strain bacillus cereus var. toyoi on enterobacterial growth and selected metabolic parameters in digesta samples from piglets before and shortly after weaning was studied. growth capacities of enterobacteria in digesta samples were significantly reduced, but no correlation was found between enterobacterial growth and concentrations of short-chain fatty acids and lactic acid, which are often influenced by other probiotic preparations. tendencies for higher amounts of s ... | 2002 | 11906572 |
| [comparative study of the elemental composition of vegetative and dormant microbial cells]. | x-ray microanalysis showed that vegetative cells, viable resting forms, and nonviable forms (micromummies) of the bacteria bacillus cereus and micrococcus luteus and the yeast saccharomyces cerevisiae differ in the contents of bioelements s, p, ca, and k and the ca/k and p/s ratios. viable resting forms (cystlike refractory cells and bacillar endospores) had more calcium and less phosphorus and potassium than vegetative cells, the difference being higher for bacilli than for micrococci and yeast ... | 2002 | 11910805 |
| rheological characteristics of microbial suspensions of pseudomonas aeruginosa and bacillus cereus. | the rheological properties of the bacteria pseudomonas aeruginosa and bacillus cereus have been investigated. the apparent viscosity of the bacterial suspensions has been measured at different conditions. the results showed that the bacterial suspensions' apparent viscosity increased with increasing biomass concentration of each of these strains. the p. aeruginosa suspension followed shear thinning behavior while b. cereus suspension followed shear thickening behavior. the shear stress versus sh ... | 2002 | 11911895 |
| role of tryptophan residues in interfacial binding of phosphatidylinositol-specific phospholipase c. | the phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus thuringiensis exhibits several types of interfacial activation. in the crystal structure of the closely related bacillus cereus pi-plc, the rim of the active site is flanked by a short helix b and a loop that show an unusual clustering of hydrophobic amino acids. two of the seven tryptophans in pi-plc are among the exposed residues. to test the importance of these residues in substrate and activator binding, we prepared sev ... | 2002 | 11912206 |
| survival and growth of potential microbial contaminants in severe environments. | studies conducted in our laboratory have established that a number of potential soil microbes could survive a simulated martian environment. in view of the uncertainty of the martian environment and the importance of noncontamination of extraterrestrial bodies, studies were performed with common soil microbes to determine the minimal environment necessary to produce a complete growth cycle. the effects of diurnal temperature cycling (+25 degrees c to -65 degrees c) and of limiting concentrations ... | 1966 | 11915886 |
| the phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen bacillus cereus. | the natural antimicrobial compound carvacrol shows a high preference for hydrophobic phases. the partition coefficients of carvacrol in both octanol-water and liposome-buffer phases were determined (3.64 and 3.26, respectively). addition of carvacrol to a liposomal suspension resulted in an expansion of the liposomal membrane. maximum expansion was observed after the addition of 0.50 micromol of carvacrol/mg of l-alpha-phosphatidylethanolamine. cymene, a biological precursor of carvacrol which l ... | 2002 | 11916669 |
| structural characterization of lipopeptide biomarkers isolated from bacillus globigii. | spectra obtained using matrix-assisted laser desorption/ionization (maldi) mass spectrometry of bacillus globigii (bacillus subtilis niger) spores, vegetative cells and the culture supernatant show a cluster of biomarkers centered at a molecular mass of 1478 da. three biomarkers were isolated from the cell-free culture supernatant by solid-phase extraction and reversed-phase high-performance liquid chromatography, and characterized using various kinds of mass spectrometry. a fourier transform ma ... | 2002 | 11921366 |
| molecular epidemiology of bacillus cereus food poisoning. | to investigate the potential use of dna techniques in epidemiological diagnosis of bacillus cereus food poisoning. | 2001 | 11921595 |
| recent developments in carbapenems. | carbapenems are beta-lactam antibiotics characterised by the presence of a beta-lactam ring with a carbon instead of sulfone in the 4-position of the thyazolidinic moiety. the first carbapenem to be utilised in therapy was imipenem, the n-formimidoyl derivative of thienamycin. imipenem is coadministered with cilastatin, an inhibitor of human renal dehydropeptidase i, as imipenem is hydrolysed by this enzyme. meropenem was the first carbapenem with a 1-beta-methyl group and 2-thio pyrrolidinyl mo ... | 2002 | 11922861 |