Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| guillain barré syndrome. | guillain barré syndrome is one of the best examples of a post infectious immune disease and offers insights into the mechanism of tissue damage in other more common autoimmune diseases. controlled epidemiological studies have linked it to infection with campylobacter jejuni in addition to other viruses including cytomegalovirus and epstein barr virus. the syndrome includes several pathological subtypes, of which the most common is a multifocal demyelinating disorder of the peripheral nerves in c ... | 2001 | 11724912 |
| genotypic and serotypic stability of campylobacter jejuni strains during in vitro and in vivo passage. | the stability of four typing methods and the sero- and genotypic stability of three campylobacter jejuni strains were evaluated after subculturing 50 times in triplicate and after colonising mice for up to 26 days. the employed methods were penner heat-stable serotyping; automated ribotyping (riboprinting) using haeiii restriction enzyme; pulsed-field gel electrophoresis (pfge) using smai, sali and kpni; and random amplified polymorphic dna analysis (rapd) using primers 1254, 1281 and hlwl85. no ... | 2001 | 11727822 |
| effect of environmental and chemotactic stimuli on the activity of the campylobacter jejuni flaa sigma(28) promoter. | the effect of environmental and chemotactic stimuli was assessed using a fusion between the campylobacter jejuni flaa sigma(28) promoter and a promoterless luxcdabe cassette on pryluxcdabe. environmental stimuli relevant to the enteric environment were found to modulate flaa promoter activity. ph, bovine bile, deoxycholate, l-fucose and osmolarity up-regulated the flaa promoter while viscosity resulted in down-regulation. c. jejuni chemotactic effectors, including the amino acids aspartate and g ... | 2001 | 11728714 |
| development of a pcr elisa assay for the identification of campylobacter jejuni and campylobacter coli. | a polymerase chain reaction (pcr) assay was developed based on a solution-hybridization colorimetric end-point detection format (pcr elisa) for the identification of campylobacter jejuni and campylobacter coli. pcr primers were designed to target a gene sequence with species-specific motifs. five biotin-labelled probes targeted to the species-specific motifs were investigated for the detection of digoxygenin-labelled pcr products from c. jejuni and c. coli using the pcr elisa format. two probes ... | 2001 | 11735301 |
| optimality of the genetic code with respect to protein stability and amino-acid frequencies. | the genetic code is known to be efficient in limiting the effect of mistranslation errors. a misread codon often codes for the same amino acid or one with similar biochemical properties, so the structure and function of the coded protein remain relatively unaltered. previous studies have attempted to address this question quantitatively, by estimating the fraction of randomly generated codes that do better than the genetic code in respect of overall robustness. we extended these results by inves ... | 2001 | 11737948 |
| an optimised recovery method for thermophilic campylobacter from liver. | the past three decades have witnessed the rise of campylobacter enteritis in man from virtual obscurity to notoriety, with present isolation rates superseding those of other enteric pathogens such as salmonella spp. and shigella spp. in most developed countries. although campylobacters are not completely new to applied bacteriology, they have evaded traditional isolation techniques used for the isolation of pure cultures, apart from single isolations that were free from competing organisms. offa ... | 2001 | 11741507 |
| plasmid-encoded autolysin in bacillus anthracis: modular structure and catalytic properties. | a bacillus anthracis virulence plasmid-encoded peptidoglycan hydrolase (amia) with sequence similarity to n-acetylmuramoyl-l-alanine amidases hydrolyzes peptidoglycan independently of cell wall binding. residues h341, e355, h415, and e486 are absolutely required for catalysis. many amia paralogs are fused to different sorting signals, suggesting that these modular proteins result from domain shuffling. | 2002 | 11741877 |
| use of a multiplex pcr-based assay to differentiate campylobacter jejuni and campylobacter coli strains isolated from human and animal sources. | 2002 | 11749144 | |
| the dioxin crisis as experiment to determine poultry-related campylobacter enteritis. | in june 1999, the dioxin crisis, caused by dioxin-contaminated feed components, exploded in belgium, resulting in withdrawal of chicken and eggs from the market. through the sentinel surveillance system, a decrease in campylobacter infections during june 1999 was noticed. a model was generated with the reports from preceding years (1994 to 1998), and a prediction of the number of infections in 1999 was calculated. the model shows a significant decline (40%) in the number of infections, mainly be ... | 2002 | 11749743 |
| biosynthesis pathway of adp-l-glycero-beta-d-manno-heptose in escherichia coli. | the steps involved in the biosynthesis of the adp-l-glycero-beta-d-manno-heptose (adp-l-beta-d-heptose) precursor of the inner core lipopolysaccharide (lps) have not been completely elucidated. in this work, we have purified the enzymes involved in catalyzing the intermediate steps leading to the synthesis of adp-d-beta-d-heptose and have biochemically characterized the reaction products by high-performance anion-exchange chromatography. we have also constructed a deletion in a novel gene, gmhb ... | 2002 | 11751812 |
| characterization of comq and comx, two genes required for production of comx pheromone in bacillus subtilis. | many microbes use secreted peptide-signaling molecules to stimulate changes in gene expression in response to high population density, a process called quorum sensing. comx pheromone is a modified 10-amino-acid peptide used by bacillus subtilis to modulate changes in gene expression in response to crowding. comq and comx are required for production of comx pheromone. we found that accumulation of comx pheromone in culture supernatant paralleled cell growth, indicating that there was no autoinduc ... | 2002 | 11751817 |
| superfamily: hmms representing all proteins of known structure. scop sequence searches, alignments and genome assignments. | the superfamily database contains a library of hidden markov models representing all proteins of known structure. the database is based on the scop 'superfamily' level of protein domain classification which groups together the most distantly related proteins which have a common evolutionary ancestor. there is a public server at http://supfam.org which provides three services: sequence searching, multiple alignments to sequences of known structure, and structural assignments to all complete genom ... | 2002 | 11752312 |
| the arabidopsis huellenlos gene, which is essential for normal ovule development, encodes a mitochondrial ribosomal protein. | the huellenlos (hll) gene participates in patterning and growth of the arabidopsis ovule. we have isolated the hll gene and shown that it encodes a protein homologous to the l14 proteins of eubacterial ribosomes. the arabidopsis genome also includes a highly similar gene, huellenlos paralog (hlp), and genes for both cytosolic (l23) and chloroplast ribosome l14 proteins. phylogenetic analysis shows that hll and hlp differ significantly from these other two classes of such proteins. hll and hlp fu ... | 2001 | 11752383 |
| dna diversity of the wla gene cluster among serotype hs:19 and non-hs:19 campylobacter jejuni strains. | campylobacter jejuni infection is an important trigger of guillain-barré syndrome (gbs), and serotype hs:19 strains are over-represented among gbs-associated isolates. structures in c. jejuni lipooligosaccharide (los) resemble human gangliosides, suggesting that molecular mimicry could be important in triggering the neural injury. we assessed the genetic diversity among 36 c. jejuni serotype hs:19 and non-hs:19 strains by analysis of pcr-based restriction fragment length polymorphism (rflp) patt ... | 2001 | 11753203 |
| [antagonistic activity of lactobacillus bacteria strains against anaerobic gastrointestinal tract pathogens (helicobacter pylori, campylobacter coli, campylobacter jejuni, clostridium difficile)]. | antagonistic activity of lactobacillus strains has been known for some time. this property is connected with production of many active substances by lactobacilli e.g., organic acids and bacteriocin-like substances which interfere with other indigenous microorganisms inhabiting the same ecological niche, including also anaerobic gastrointestinal tract pathogens. growing interest of clinical medicine in finding new approaches to treatment and prevention of common inflammatory infections of the dig ... | 2001 | 11757422 |
| single or double mutational alterations of gyra associated with fluoroquinolone resistance in campylobacter jejuni and campylobacter coli. | we looked for the presence of gyra mutations in seven fluoroquinolone-resistant french clinical isolates of campylobacter jejuni and campylobacter coli. three of the five isolates of c. jejuni and the two isolates of c. coli had high-level resistance to nalidixic acid (mics 128-256 microg/ml) and ciprofloxacin (mics 32 microg/ml). a gyra mutation was found in all these isolates leading to the following substitutions: thr86-ile in four cases and asp90-tyr for one c. coli strain. one isolate had h ... | 2001 | 11759087 |
| molecular detection of campylobacter spp. in drinking, recreational and environmental water supplies. | a molecular detection assay was performed on 207 samples of drinking, recreational and environmental waters collected in northern ireland. the water sources which were pcr positive for campylobacter spp. included 2/91 (2.2%) drinking water from domestic household taps, 5/57 (8.8%) swimming pool water, 1/23 (4.3%) lake water and 1/1 water from a jacuzzi. extracted dna from all water samples was amplified employing a sequence-specific pcr assay based on a 206 bp conserved region of the flagellin a ... | 2001 | 11759163 |
| isolation and polymerase chain reaction-based detection of campylobacter jejuni and campylobacter coli from poultry in the philippines. | the polymerase chain reaction (pcr) and the conventional culture method of detecting thermophilic campylobacter species in duck and chicken samples from two locations in the province of laguna, philippines, were compared. three campylobacter jejuni and five c. coli strains were isolated from a total of 135 duck and chicken samples from both methods. the pcr technique, however, was found to be more sensitive, accurate and rapid than the conventional culture method. the specificity of two sets of ... | 2001 | 11759758 |
| role of gancyclovir and haart administration in the treatment of a rare complication of hiv disease: cytomegalovirus-associated guillain-barré syndrome. | peripheral nerve disorders are very common in patients with hiv infection, including inflammatory demyelinating polyneuropathies, such as guillain-barré syndrome. causes of these neuropathies are probably multiple, and often dictated by the stage of the underlying hiv disease. acute demyelinating polyneuropathy is usually preceded by infections, generally sustained by cytomegalovirus or campylobacter jejuni, and a co-infection with hiv may represent the initial etiopathogenetic event leading to ... | 2001 | 11760224 |
| comparison of smai-defined genotypes of campylobacter jejuni examined by kpni: a population-based study. | pulsed-field gel electrophoresis (pfge) was used to analyse 147 isolates collected in two regions of quebec province (estrie and montreal) between march 1998 and feb. 1999, to determine the utility of molecular strain typing for a population-based collection of campylobacter jejuni and to compare directly the discriminatory power of smai and kpni restriction digests. with a combination of epidemiological criteria including space and time plus molecular strain typing, 49% of isolates from estrie ... | 2001 | 11761192 |
| campylobacter species associated with diarrhoea in patients from a tertiary care centre of north india. | most laboratories do not routinely distinguish the various campylobacter species, though almost all campylobacter species have been isolated from human faeces. the epidemiological and clinical aspects of its infection and the species involved in genesis of diarrhoea are least understood in the developing countries. the aim of the present study was to find out frequency of campylobacter species isolated from patients with diarrhoea over a 12-year period and to analyse their features. | 2001 | 11762201 |
| campylobacter jejuni. | 2001 | 11764732 | |
| use of luminescent campylobacter jejuni atcc 33291 to assess eggshell colonization and penetration in fresh and retail eggs. | a luminescent phenotype in campylobacter jejuni atcc 33291, generated by a transcriptional fusion between the c. jejuni flaa sigma28 promoter and the luxcdabe genes of xenorhabdus luminescens on plasmid pryluxcdabe, was used to examine colonization and penetration of fresh and retail eggs. c. jejuni colonized both fresh and retail eggs at 37, 40, and 42 degrees under microaerophilic conditions. fresh eggs were more heavily colonized than retail eggs. under aerobic conditions, fresh eggs were col ... | 2001 | 11770638 |
| effect of cold starvation, acid stress, and nutrients on metabolic activity of helicobacter pylori. | helicobacter pylori can transform, in vivo as well as in vitro, from dividing spiral-shaped forms into nonculturable coccoids, with intermediate forms called u forms. the importance of nonculturable coccoid forms of h. pylori in disease transmission and antibiotic treatment failures is unclear. metabolic activities of actively growing as well as nonculturable h. pylori were investigated by comparing the concentrations of cellular atp and total rna, gene expression, presence of cytoplasmic polyph ... | 2002 | 11772603 |
| 16s rrna-based analysis of microbiota from the cecum of broiler chickens. | the microbiota of the intestinal tract of chickens plays an important role in inhibiting the establishment of intestinal pathogens. earlier culturing and microscopic examinations indicated that only a fraction of the bacteria in the cecum of chickens could be grown in the laboratory. therefore, a survey of cecal bacteria was done by retrieval of 16s rrna gene sequences from dna isolated from the cecal content and the cecal mucosa. the ribosomal gene sequences were amplified with universal primer ... | 2002 | 11772618 |
| occurrence of cryptosporidium and giardia in wild ducks along the rio grande river valley in southern new mexico. | fecal samples were taken from wild ducks on the lower rio grande river around las cruces, n. mex., from september 2000 to january 2001. giardia cysts and cryptosporidium oocysts were purified from 69 samples by sucrose enrichment followed by cesium chloride (cscl) gradient centrifugation and were viewed via fluorescent-antibody (fa) staining. for some samples, recovered cysts and oocysts were further screened via pcr to determine the presence of giardia lamblia and crytosporidium parvum. the res ... | 2002 | 11772622 |
| multiplex lightcycler pcr assay for detection and differentiation of bordetella pertussis and bordetella parapertussis in nasopharyngeal specimens. | a rapid real-time multiplex pcr assay for detecting and differentiating bordetella pertussis and bordetella parapertussis in nasopharyngeal swabs was developed. this assay (lc-pcr-is) targets the insertion sequences is481 and is1001 of b. pertussis and b. parapertussis, respectively, and is performed using the lightcycler (roche molecular biochemicals, indianapolis, ind.). the analytical sensitivity is less than one organism per reaction. results for bordetella culture and/or direct fluorescent ... | 2002 | 11773099 |
| evaluation of a novel heminested pcr assay based on the phosphoglucosamine mutase gene for detection of helicobacter pylori in saliva and dental plaque. | a novel heminested pcr protocol was developed for the specific detection of helicobacter pylori at low copy numbers. a set of primers specific for the phosphoglucosamine mutase gene (glmm) of h. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a 496-bp fragment. by using agarose gel electrophoresis for detection of the heminested pcr-amplified products, amplification of h. pylori genomic dna was achieved at concentrations as low as 0.1 pg, eq ... | 2002 | 11773117 |
| hla alleles in patients with guillain-barre syndrome. | to determine whether genetic factors may work in concert with autoimmune factors in the pathogenesis of guillain-barre syndrome (gbs). | 2000 | 11776098 |
| production and application of new monoclonal antibodies specific for a fecal helicobacter pylori antigen. | the aim of the present study was to establish monoclonal antibodies that could be used to produce a diagnostic test composed of one kind of monoclonal antibody recognizing a fecal helicobacter pylori antigen. the need to develop such a test arose from disadvantages of the diagnostic test that uses a polyclonal antibody or plural kinds of monoclonal antibodies, such as the lower specificity for h. pylori antigen and the difficulty of reproduction with consistent quality. mice were immunized with ... | 2002 | 11777832 |
| monoclonal antibodies to immunodominant epitope of tropheryma whipplei. | recent isolation of tropheryma whipplei (formerly trophyrema whippelii), the agent of whipple's disease, from the cardiac valve of a patient with whipple's disease endocarditis now allows the detection of reactive epitopes that could be used in a serological assay. in order to propose an enzyme-linked immunosorbent assay (elisa) that uses recombinant t. whipplei antigen, we first determined by western blotting of human, mouse, and rabbit antisera that the common immunodominant epitope is an 84-k ... | 2002 | 11777846 |
| the crystal structure of helicobacter pylori cysteine-rich protein b reveals a novel fold for a penicillin-binding protein. | colonization of the gastric mucosa with the spiral-shaped gram-negative proteobacterium helicobacter pylori is probably the most common chronic infection in humans. the genomes of h. pylori strains j99 and 26695 have been completely sequenced. functional and three-dimensional structural information is available for less than one third of all open reading frames. we investigated the function and three-dimensional structure of a member from a family of cysteine-rich hypothetical proteins that are ... | 2002 | 11777911 |
| miller fisher syndrome: toward a more comprehensive understanding. | to review recent knowledge on the clinical features, pathology and pathophysiology, diagnosis and treatment of miller fisher syndrome (mfs). | 2001 | 11780304 |
| the non-h pylori helicobacters: their expanding role in gastrointestinal and systemic diseases. | the number of species in the genus helicobacter has rapidly expanded over the past decade. the genus now includes at least 24 formally named species as well as numerous other helicobacters awaiting formal naming. this review highlights the expanding role that other helicobacters, although not as well known as h pylori, play in gastrointestinal and systemic disease in humans. | 2002 | 11788573 |
| genome sequence of the hyperthermophilic crenarchaeon pyrobaculum aerophilum. | we determined and annotated the complete 2.2-megabase genome sequence of pyrobaculum aerophilum, a facultatively aerobic nitrate-reducing hyperthermophilic (t(opt) = 100 degrees c) crenarchaeon. clues were found suggesting explanations of the organism's surprising intolerance to sulfur, which may aid in the development of methods for genetic studies of the organism. many interesting features worthy of further genetic studies were revealed. whole genome computational analysis confirmed experiment ... | 2002 | 11792869 |
| rgg influences the expression of multiple regulatory loci to coregulate virulence factor expression in streptococcus pyogenes. | the human pathogen streptococcus pyogenes secretes many proteins to the cell wall and extracellular environment that contribute to virulence. rgg regulates the expression of several exoproteins including a cysteine protease (spe b), a nuclease (mf-1), a putative nuclease (mf-3), and autolysin. the functional heterogeneity of rgg-regulated exoproteins and the lack of a conserved regulatory motif in the promoter regions of the genes suggested that rgg interacts with additional regulatory networks ... | 2002 | 11796609 |
| phase variation of campylobacter jejuni 81-176 lipooligosaccharide affects ganglioside mimicry and invasiveness in vitro. | the outer cores of the lipooligosaccharides (los) of many strains of campylobacter jejuni mimic human gangliosides in structure. a population of cells of c. jejuni strain 81-176 produced a mixture of los cores which consisted primarily of structures mimicking gm(2) and gm(3) gangliosides, with minor amounts of structures mimicking gd(1b) and gd(2). genetic analyses of genes involved in the biosynthesis of the outer core of c. jejuni 81-176 revealed the presence of a homopolymeric tract of g resi ... | 2002 | 11796612 |
| rapid activation of protein tyrosine kinase and phospholipase c-gamma2 and increase in cytosolic free calcium are required by ehrlichia chaffeensis for internalization and growth in thp-1 cells. | ehrlichia chaffeensis, a bacterium that cannot survive outside the eukaryotic cell, proliferates exclusively in human monocytes and macrophages. in this study, signaling events required for ehrlichial infection of human monocytic cell line thp-1 were characterized. entry and proliferation of e. chaffeensis in thp-1 cells were significantly blocked by various inhibitors that can regulate calcium signaling, including 8-(diethylamino)octyl-3,4,5-trimethoxybenzoate and 2-aminoethoxydiphenyl borate ( ... | 2002 | 11796624 |
| thermophilic campylobacters in surface water: a potential risk of campylobacteriosis. | campylobacteriosis caused by campylobacter jejuni, campylobacter coli and campylobacter lari is one of the most frequently occurring acute gastroenteritis diseases in humans. an important risk factor is untreated drinking water. thermotolerant campylobacters were isolated from bø river water using 100-ml filtrate samples. campylobacters were isolated from 32 of 60 samples (53.3%). of the 75 strains isolated, 47 (62.7%) were detected using the enrichment procedure and 28 (37.2%) by direct plating ... | 2001 | 11798419 |
| [the relationship between guillain-barre syndrome and serotypes of campylobacter jejuni isolated from the patients]. | to investigate the relationship between campylobacter jejuni (cj) serotype and the high incidence of guillain-barre syndrome (gbs) following cj enteritis in china. | 1999 | 11798680 |
| development of a combined filtration-enrichment culture followed by a one-step duplex pcr technique for the rapid detection of campylobacter jejuni and c. coli in human faecal samples. | a new combined filtration-enrichment culture followed by a pcr technique for the rapid detection of campylobacterjejuni and c. coli in human faeces has been developed. only bacteria that passed through the membrane could multiply in the enrichment culture; target bacteria were detected by a one-step duplex pcr technique with combinations of primers that are specific for different campylobacter spp., which should allow for the detection of a mixed infection in a single patient. a falcon cell-cult ... | 2002 | 11800478 |
| succinate:quinone oxidoreductases from epsilon-proteobacteria. | the epsilon-proteobacteria form a subdivision of the proteobacteria including the genera wolinella, campylobacter, helicobacter, sulfurospirillum, arcobacter and dehalospirillum. the majority of these bacteria are oxidase-positive microaerophiles indicating an electron transport chain with molecular oxygen as terminal electron acceptor. however, numerous members of the epsilon-proteobacteria also grow in the absence of oxygen. the common presence of menaquinone and fumarate reduction activity su ... | 2002 | 11803019 |
| a genome-scale analysis for identification of genes required for growth or survival of haemophilus influenzae. | a high-density transposon mutagenesis strategy was applied to the haemophilus influenzae genome to identify genes required for growth or viability. this analysis detected putative essential roles for the products of 259 orfs of unknown function. comparisons between complete genomes defined a subset of these proteins in h. influenzae having homologs in mycobacterium tuberculosis that are absent in saccharomyces cerevisiae, a distribution pattern that favors their use in development of antimicrobi ... | 2002 | 11805338 |
| significance of two distinct types of tryptophan synthase beta chain in bacteria, archaea and higher plants. | tryptophan synthase consists of two subunits, alpha and beta. two distinct subgroups of beta chain exist. the major group (trpeb_1) includes the well-studied beta chain of salmonella typhimurium. the minor group of beta chain (trpeb_2) is most frequently found in the archaea. most of the amino-acid residues important for catalysis are highly conserved between both trpe subfamilies. | 2002 | 11806827 |
| significance of two distinct types of tryptophan synthase beta chain in bacteria, archaea and higher plants. | tryptophan synthase consists of two subunits, alpha and beta. two distinct subgroups of beta chain exist. the major group (trpeb_1) includes the well-studied beta chain of salmonella typhimurium. the minor group of beta chain (trpeb_2) is most frequently found in the archaea. most of the amino-acid residues important for catalysis are highly conserved between both trpe subfamilies. | 2002 | 11806827 |
| pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm. | complementary approaches were employed to characterize transitional episodes in pseudomonas aeruginosa biofilm development using direct observation and whole-cell protein analysis. microscopy and in situ reporter gene analysis were used to directly observe changes in biofilm physiology and to act as signposts to standardize protein collection for two-dimensional electrophoretic analysis and protein identification in chemostat and continuous-culture biofilm-grown populations. using these approach ... | 2002 | 11807075 |
| campylobacter dna is present in circulating myelomonocytic cells of healthy persons and in persons with guillain-barré syndrome. | campylobacter jejuni is the prime cause of foodborne bacterial gastroenteritis. an important complication of c. jejuni enteritis is guillain-barré syndrome (gbs), an immune-mediated disorder of the peripheral nerve. the presence of c. jejuni dna in peripheral blood mononuclear cells (pbmc) of patients with gbs, patients with c. jejuni enteritis, and healthy subjects was studied. two target genes, the flagellin and the ceue genes, were used for polymerase chain reaction (pcr) identification of ca ... | 2002 | 11807702 |
| prevalence and comparison of genetic profiles of campylobacter strains isolated from poultry and sporadic cases of campylobacteriosis in humans. | between july 1998 and june 1999, 93 lots of broiler chickens distributed on 57 farms were sampled in two abattoirs of the province of quebec (canada). a total of 2,325 samples of cecal material were analyzed to determine the prevalence of campylobacters. biotyping and pulsed-field gel electrophoresis (pfge) were done on 20% of the campylobacter isolates to study the distribution within poultry production. macrorestriction profiles were compared with profiles of 24 campylobacter strains isolated ... | 2002 | 11808809 |
| identification and molecular characterisation of cmeb, a campylobacter jejuni multidrug efflux pump. | a multidrug efflux pump gene (cmeb) was identified from the published campylobacter jejuni genome sequence. secondary structural analysis showed that the gene encoded a protein belonging to the resistance nodulation cell division (rnd) family of efflux transporters. the gene was inactivated by insertional mutagenesis. compared with the wild-type strain (nctc 11168), the resultant knockout strain (nctc 11168-cmeb::kan(r)) displayed increased susceptibility to a range of antibiotics including beta ... | 2002 | 11814661 |
| inflammatory bowel disease: definition, epidemiology, etiologic aspects, and immunogenetic studies. | 1998 | 11819343 | |
| etiology and evaluation of diarrhea in aids:a global perspective at the millennium. | 2000 | 11819553 | |
| agglutination of helicobacter pylori coccoids by lectins. | aim:to study the agglutination pattern of helicobacter pylori coccoid andspiral forms.methods:assays of agglutination and agglu-tination inhibition were applied using fifteen commercial lectins.results:strong agglutination was observed with mannose specific concanavalin a(con a), fucose specific tetragonolobus purpureas(lotus a) and n-acetyl glucosamine-specific triticum vulgaris (wga) lectins. mannose and fucose specific lectins were reactive with all strains of h. pylori coccoids as compared t ... | 2000 | 11819557 |
| alterations in gastric mucin synthesis by helicobacter pylori. | 2000 | 11819633 | |
| therapeutic methods for diarrhea in children. | 2000 | 11819636 | |
| cloning of the o-acetylserine lyase gene from the ruminal bacterium selenomonas ruminantium hd4. | the gene coding for o-acetylserine lyase (oasl) was cloned from a selenomonas ruminantium hd4 lambda zap ii genomic library by degenerative probe hybridization and complementation. sequence analysis revealed a 933 bp orf with a g + c content of 53%. the orf had significant homology with enzymes involved in cysteine biosynthesis. a curablastn homology search showed that the orf shared 59% nucleotide identity with the cysk of bacillus subtilis. the deduced amino acid sequence exhibited high (>70%) ... | 2002 | 11821922 |
| inoculation of chicks with viable non-colonizing strains of campylobacter jejuni: evaluation of protection against a colonizing strain. | we have treated chicks with viable non-colonizing mutant strains of campylobacter jejuni to test these as a possible vaccine. we found that intramuscular inoculation with and without adjuvant, and with or without a concomitant oral dose of non-colonizing strains, failed to provoke protective immunity. | 2002 | 11821932 |
| impact of transport crate reuse and of catching and processing on campylobacter and salmonella contamination of broiler chickens. | the influence of transport, catching, and processing on contamination of broiler chickens with salmonella and campylobacter was investigated. transport crates were reused with high frequency and were often still contaminated with salmonella and campylobacter when they arrived at the farm despite the fact that they were washed at the factory, and thus they were a potential route of infection. these organisms contaminated the feathers of previously campylobacter- and salmonella-negative birds goin ... | 2002 | 11823211 |
| novel antigens of helicobacter pylori correspond to ulcer-related antibody pattern of sera from infected patients. | recently, we reported that the patterns of antibodies to helicobacter pylori protein antigens in serum may be useful for screening patients at high risk for ulcers (p. aucher et al., j. clin. microbiol. 36:931-936, 1998). here we report the identification, by a combination of electrophoretic, immunochemical, and protein sequencing methods, of five antigens that correspond to this antibody pattern: groel, catalase a, flagellin a, beta-ketoacyl-acyl carrier protein synthase i (beta-ketoacyl-acp s) ... | 2002 | 11825970 |
| lectin typing of campylobacter concisus. | a total of 44 clinical isolates and the type strain of the putative pathogen campylobacter concisus were grouped based on their reactions with plant lectins. the optimized lectin typing system used c. concisus strains proteolytically pretreated and subsequently typed by using a panel of four lectins. the system grouped all 45 strains into 13 lectin reaction patterns, leaving no strain untypeable due to autoagglutination. lectin types were both stable and reproducible. | 2002 | 11826005 |
| a novel paralogous gene family involved in phase-variable flagella-mediated motility in campylobacter jejuni. | flagella-mediated motility is recognized to be one of the major factors contributing to virulence in campylobacter jejuni. motility of this bacterium is known to be phase variable, although the mechanism of such variation remains unknown. c. jejuni genome sequencing revealed a number of genes prone to phase variation via a slipped-strand mispairing mechanism. many of these genes are hypothetical and are clustered in the regions involved in formation of three major cell surface structures: capsul ... | 2002 | 11832511 |
| antimicrobial effect of electrolyzed water for inactivating campylobacter jejuni during poultry washing. | the effectiveness of electrolyzed (eo) water for killing campylobacter jejuni on poultry was evaluated. complete inactivation of c. jejuni in pure culture occurred within 10 s after exposure to eo or chlorinated water, both of which contained 50 mg/l of residual chlorine. a strong bactericidal activity was also observed on the diluted eo water (containing 25 mg/l of residual chlorine) and the mean population of c. jejuni was reduced to less than 10 cfu/ml (detected only by enrichment for 48 h) a ... | 2002 | 11843416 |
| a multiplex polymerase chain reaction for the differentiation of campylobacter jejuni and campylobacter coli from a swine processing facility and characterization of isolates by pulsed-field gel electrophoresis and antibiotic resistance profiles. | a multiplex polymerase chain reaction (pcr) was developed for the detection and speciation of 60 campylobacter strains isolated from porcine rectal swabs and from different areas in a pork processing plant. the pcr assay was based on primers specific for the cadf gene of pathogenic campylobacter species, a specific but undefined gene of campylobacter jejuni, and the ceue gene of campylobacter coli. further characterization of these isolates was established by pulsed-field gel electrophoresis (pf ... | 2002 | 11848556 |
| validation of a polymerase chain reaction/restriction enzyme analysis method for species identification of thermophilic campylobacters isolated from domestic and wild animals. | to compare and evaluate a polymerase chain reaction/restriction enzyme analysis (pcr/rea) method with standard phenotypic tests for the identification and differentiation of the thermophilic campylobacters campylobacter jejuni, c. coli, c. lari and c. upsaliensis. | 2002 | 11849327 |
| survival of campylobacter jejuni on beef trimmings during freezing and frozen storage. | to investigate the survival of two animal isolates of campylobacter jejuni on beef trimmings during freezing and frozen storage. | 2002 | 11849497 |
| antimicrobial resistance and plasmid profiles of campylobacter jejuni and campylobacter coli from human and animal sources. | the purpose of this study was to determine the susceptibility of campylobacter jejuni and campylobacter coli isolates to antimicrobial agents and to investigate the presence of plasmid dna. | 2002 | 11849513 |
| structure of campylobacter jejuni lipopolysaccharides determines antiganglioside specificity and clinical features of guillain-barré and miller fisher patients. | ganglioside mimicry in the lipopolysaccharide (lps) fraction of campylobacter jejuni isolated from guillain-barré syndrome (gbs) and miller fisher syndrome (mfs) patients was compared with isolates from patients with an uncomplicated enteritis. the antibody response to c. jejuni lps and gangliosides in neuropathy patients and controls was compared as well. lps from gbs and mfs-associated isolates more frequently contained ganglioside-like epitopes compared to control isolates. almost all neuropa ... | 2002 | 11854201 |
| further increase in ciprofloxacin-resistant campylobacter jejuni/coli in styria, austria. | 1999 | 11856217 | |
| subspecies characterization of porcine campylobacter coli and campylobacter jejuni by multilocus enzyme electrophoresis typing. | enteropathogenic campylobacterjejuni, c. coli and c. lari are currently the most common causes of acute infectious diarrhoeal illness in the uk. many domestic animals, including pigs, act as natural reservoirs of these organisms and infection may occur through the ingestion of contaminated foodstuffs. c jejuni and c. coli, isolated from the livers of bacon pigs, were examined at subspecies level by multilocus enzyme electrophoresis (mee) typing with seven enzymic loci. polymorphological variatio ... | 2002 | 11860082 |
| t cell recognition of a non-protein antigen preparation of campylobacter jejuni in patients with guillain-barré syndrome. | 2002 | 11861714 | |
| identification and comparative analysis of the chloroplast alpha-subunit gene of dna-dependent rna polymerase from seven euglena species. | when the sequence of the euglena gracilis chloroplast genome was reported in 1993 the alpha-subunit gene (rpoa) of rna polymerase appeared to be missing, based on a comparison of all putative reading frames to the then known rpoa loci. since there has been a large increase in known rpoa sequences, the question of a euglena chloroplast rpoa gene was re-examined. a previously described unknown reading frame of 161 codons was found to be part of an rpoa gene split by a single group iii intron. this ... | 2002 | 11861918 |
| water-borne campylobacter jejuni infection in a danish town---a 6-week continuous source outbreak. | objective: to determine the cause and characteristics of illness of a campylobacter jejuni outbreak in denmark in 1995--96. methods: a retrospective follow-up study was designed for culture-confirmed cases and for residents without a bacteriologic diagnosis. stored clinical and environmental isolates were analyzed by serotyping and genotyping with restriction endonuclease analysis (rea), pulsed-field gel electrophoresis (pfge) and ribotyping. results: campylobacter jejuni was isolated from 110 r ... | 1998 | 11864264 |
| the past, present and future of genome-wide re-annotation. | annotation, the process by which structural or functional information is inferred for genes or proteins, is crucial for obtaining value from genome sequences. we define the process of annotating a previously annotated genome sequence as 're-annotation', and examine the strengths and weaknesses of current manual and automatic genome-wide re-annotation approaches. | 2002 | 11864365 |
| [a case-control study on guillain-barre syndrome in children of north china]. | this study aimed at exploring the risk factors for guillain-barre syndrome (gbs). | 1999 | 11864491 |
| infectious origins of, and molecular mimicry in, guillain-barré and fisher syndromes. | guillain-barré syndrome (gbs), characterised by limb weakness and areflexia, is the prototype of postinfectious autoimmune diseases, and campylobacter jejuni is the most frequent antecedent pathogen. gbs subsequent to c jejuni enteritis is associated with a severe, pure motor axonal variant and igg antibodies against gm1, gm1b, gd1a, or galnac-gdla, gangliosides expressed in human peripheral nerves. lipopolysaccharides of c jejuni isolated from gbs patients have ganglioside-like epitopes. cytome ... | 2001 | 11871407 |
| detection of campylobacter jejuni and campylobacter coli in environmental waters by pcr enzyme-linked immunosorbent assay. | a pcr enzyme-linked immunosorbent assay (elisa) assay was applied to the detection of campylobacter jejuni and campylobacter coli in environmental water samples after enrichment culture. bacterial cells were concentrated from 69 environmental water samples by using filtration, and the filtrates were cultured in campylobacter blood-free broth. after enrichment culture, dna was extracted from the samples by using a rapid-boiling method, and the dna extracts were used as a template in a pcr elisa a ... | 2002 | 11872483 |
| identification and functional characterization of flgm, a gene encoding the anti-sigma 28 factor in pseudomonas aeruginosa. | we describe here the functional characterization of the putative flgm gene of pseudomonas aeruginosa. flgm of p. aeruginosa is most similar to flgm of vibrio parahaemolyticus. a conserved region is present in the c-terminal half of the flgm of p. aeruginosa and in flgm homologues of other organisms that includes the sigma(28) binding domain. a role for the flgm gene of p. aeruginosa in motility was demonstrated by its inactivation. the beta-galactosidase activity of a transcriptional fusion of t ... | 2002 | 11872701 |
| gene3d: structural assignment for whole genes and genomes using the cath domain structure database. | we present a novel web-based resource, gene3d, of precalculated structural assignments to gene sequences and whole genomes. this resource assigns structural domains from the cath database to whole genes and links these to their curated functional and structural annotations within the cath domain structure database, the functional dictionary of homologous superfamilies (dhs) and pdbsum. currently gene3d provides annotation for 36 complete genomes (two eukaryotes, six archaea, and 28 bacteria). on ... | 2002 | 11875040 |
| alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network. | alpha-crystallins were originally recognized as proteins contributing to the transparency of the mammalian eye lens. subsequently, they have been found in many, but not all, members of the archaea, bacteria, and eucarya. most members of the diverse alpha-crystallin family have four common structural and functional features: (i) a small monomeric molecular mass between 12 and 43 kda; (ii) the formation of large oligomeric complexes; (iii) the presence of a moderately conserved central region, the ... | 2002 | 11875128 |
| re-annotation of genome microbial coding-sequences: finding new genes and inaccurately annotated genes. | analysis of any newly sequenced bacterial genome starts with the identification of protein-coding genes. despite the accumulation of multiple complete genome sequences, which provide useful comparisons with close relatives among other organisms during the annotation process, accurate gene prediction remains quite difficult. a major reason for this situation is that genes are tightly packed in prokaryotes, resulting in frequent overlap. thus, detection of translation initiation sites and/or selec ... | 2002 | 11879526 |
| identification of campylobacter heat-stable and heat-labile antigens by combining the penner and lior serotyping schemes. | 2002 | 11880386 | |
| molecular typing of selected enterococcus faecalis isolates: pilot study using multilocus sequence typing and pulsed-field gel electrophoresis. | the present study compared the recently developed multilocus sequence typing (mlst) approach with a well-established molecular typing technique, pulsed-field gel electrophoresis (pfge), for subspecies differentiation of enterococcus faecalis isolates. we sequenced intragenic regions of three e. faecalis antigen-encoding genes (ace, encoding a collagen and laminin adhesin; efaa, encoding an endocarditis antigen; and sala, encoding a cell wall associated antigen) and one housekeeping gene (pyrc) o ... | 2002 | 11880407 |
| application of a 5' nuclease assay for detection of lawsonia intracellularis in fecal samples from pigs. | a 5' nuclease assay was developed to detect lawsonia intracellularis in porcine fecal samples. the specific probe and primers were chosen by using the 16s ribosomal dna gene as a target. the 5' nuclease assay was used with a total of 204 clinical samples, and the results were compared to those of immunohistochemistry (im) on ileal sections of the same animals. there was 91% agreement between the results of im and the 5' nuclease assay. in the 5' nuclease assay, 111 (54%) of the pigs tested posit ... | 2002 | 11880427 |
| detection of heat-stable antigens of campylobacter jejuni and c. coli by direct agglutination and passive hemagglutination. | the two serotyping schemes for the detection of heat-stable antigens of campylobacter jejuni and campylobacter coli use the same strains for antiserum production but differ in the detection systems used for identifying agglutination. the penner method uses passive hemagglutination (pha) while the laboratory of enteric pathogens method uses the same antisera but in a whole-bacterial-cell direct agglutination (da) protocol. c. jejuni produces a polysaccharide capsule, which is antigenic, and is th ... | 2002 | 11880429 |
| fatal case of campylobacter lari prosthetic joint infection and bacteremia in an immunocompetent patient. | campylobacter lari is an infrequent cause of intestinal and extraintestinal infection in humans. we report a case of c. lari prosthetic joint infection and bacteremia in an 81-year-old immunocompetent man. the infection was associated with septic shock and fatal outcome. c. lari may cause severe disease, even in an immunocompetent host. | 2002 | 11880437 |
| monoclonal antibodies specific for hippurate hydrolase of campylobacter jejuni. | eleven monoclonal antibodies raised against recombinant campylobacter jejuni hippurate hydrolase were tested for binding to lysates from 19 c. jejuni strains, 12 other campylobacter strains, and 21 non-campylobacter strains. several monoclonal antibodies bound to c. jejuni but not to other campylobacter species and may be useful in a species-specific immunoassay. | 2002 | 11880445 |
| analysis of gluconeogenic and anaplerotic enzymes in campylobacter jejuni: an essential role for phosphoenolpyruvate carboxykinase. | campylobacter jejuni is unable to utilize glucose as a carbon source due to the absence of the key glycolytic enzyme 6-phosphofructokinase. the genome sequence of c. jejuni nctc 11168 indicates that homologues of all the appropriate enzymes for gluconeogenesis from phosphoenolpyruvate (pep) are present, in addition to the anaplerotic enzymes pyruvate carboxylase (pyc), phosphoenolpyruvate carboxykinase (pck) and malic enzyme (mez). surprisingly, a pyruvate kinase (pyk) homologue is also present. ... | 2002 | 11882702 |
| variation in numbers of bacteria on paired chicken carcass halves. | bacterial counts from paired broiler carcass halves were examined for relationships between numbers and kinds of bacteria that might indicate fecal contamination. broiler carcasses removed from a commercial processing plant just before chilling were split aseptically along the midline, and each side was rinsed in 400 ml of phosphate buffered saline for 1 min with either mechanical or hand shaking. both halves of six carcasses were rinsed on four different days for a total of 24 carcasses sampled ... | 2002 | 11885892 |
| mutations in poli but not mutslh destabilize haemophilus influenzae tetranucleotide repeats. | haemophilus influenzae (hi), an obligate upper respiratory tract commensal/pathogen, uses phase variation (pv) to adapt to host environment changes. switching occurs by slippage of nucleotide repeats (microsatellites) within genes coding for virulence molecules. most such microsatellites in hi are tetranucleotide repeats, but an exception is the dinucleotide repeats in the pilin locus. to investigate the effects on pv rates of mutations in genes for mismatch repair (mmr), insertion/deletion muta ... | 2002 | 11889052 |
| bacteriological characteristics of dressed young pigeon (squabs) columba livia domesticus. | a total of 50 frozen squabs carcasses were collected from different retail markets in cairo and giza governorates. the collected samples were examined bacteriologically. the aerobic plate count, the enterobacteriaceae and the staphylococcus count were 6.6 x 10(5), 6.3 x 10(2) and 1.4 x 10(3) cfu./gram, respectively. the results revealed no positive samples for salmonella yersinia entercolitica, listeria monocytogenes and campylobacter jejuni. staphylococcus aureus was isolated from 2% of samples ... | 2002 | 11890056 |
| identification of motility and autoagglutination campylobacter jejuni mutants by random transposon mutagenesis. | campylobacter jejuni has been identified as the leading cause of acute bacterial diarrhea in the united states, yet compared with other enteric pathogens, considerably less is understood concerning the virulence factors of this human pathogen. a random in vivo transposon mutagenesis system was recently developed for the purpose of creating a library of c. jejuni transformants. a total of 1,065 c. jejuni transposon mutants were screened for their ability to swarm on motility agar plates and autoa ... | 2002 | 11895937 |
| role of flagella in host cell invasion by burkholderia cepacia. | burkholderia cepacia is an important opportunistic human pathogen that affects immunocompromised individuals, particularly cystic fibrosis (cf) patients. colonization of the lungs of a cf patient by b. cepacia can lead not only to a decline in respiratory function but also to an acute systemic infection, such as bacteremia. we have previously demonstrated that a cf clinical isolate of b. cepacia, strain j2315, can invade and survive within cultured respiratory epithelial cells. in order to furth ... | 2002 | 11895941 |
| helicobacter pylori uses motility for initial colonization and to attain robust infection. | helicobacter pylori has been shown to require flagella for infection of the stomach. to analyze whether flagella themselves or motility is needed by these pathogens, we constructed flagellated nonmotile mutants. this was accomplished by using both an insertion mutant and an in-frame deletion of the motb gene. in vitro, these mutants retain flagella (fla(+)) but are nonmotile (mot(-)). by using fvb/n mice, we found that these mutants had reduced ability to infect mice in comparison to that of the ... | 2002 | 11895962 |
| campylobacter protein glycosylation affects host cell interactions. | campylobacter jejuni 81-176 pgl mutants impaired in general protein glycosylation showed reduced ability to adhere to and invade int407 cells and to colonize intestinal tracts of mice. | 2002 | 11895996 |
| efficient isolation of campylobacters from stools: what are we missing? | 2002 | 11896083 | |
| traditional and molecular techniques for the study of emerging bacterial diseases: one laboratory's perspective. | identification of emerging bacterial pathogens generally results from a chain of events involving microscopy, serology, molecular tools, and culture. because of the spectacular molecular techniques developed in the last decades, some authors think that these techniques will shortly supplant culture. the key steps that led to the discovery of emerging bacteria have been reviewed to determine the real contribution of each technique. historically, microscopy has played a major role. serology provid ... | 2002 | 11897062 |
| current status of antimicrobial resistance in taiwan. | while some trends in antimicrobial resistance rates are universal, others appear to be unique for specific regions. in taiwan, the strikingly high prevalence of resistance to macrolides and streptogramin in clinical isolates of gram-positive bacteria correlates with the widespread use of these agents in the medical and farming communities, respectively. the relatively low rate of enterococci that are resistant to glycopeptide does not parallel the high use of glycopeptides and extended-spectrum ... | 2002 | 11897063 |
| antibodies against gangliosides: a link between preceding infection and immunopathogenesis of guillain-barré syndrome. | autoantibodies against gangliosides gm1 and gq1b, characteristic cell surface glycolipids of the nervous system, are present in specific clinical types of guillainbarré syndrome (gbs). close associations of anti-gm1 with acute motor axonal neuropathy, and of anti-gq1b with miller fisher syndrome, strongly suggest that these antibodies contribute to neuropathy pathogenesis. immune responses against gangliosides are suspected to originate as a result of molecular mimicry between gangliosides and l ... | 2002 | 11909748 |
| acute small fibre sensory neuropathy: another variant of guillain-barré syndrome? | six patients who presented with acute sensory neuropathy were studied. all patients underwent detailed clinical assessment along with electrophysiological tests and relevant laboratory investigations. all patients had acute onset numbness, reaching the peak deficit within 4 weeks. four of them had associated burning dysaesthesia. an antecedent illness was reported in four; diarrhoea in three, and urinary tract infection in one. the neurological examination disclosed normal muscle strength, symme ... | 2002 | 11909922 |
| exploiting genome sequence: predictions for mechanisms of campylobacter chemotaxis. | the genome sequence of campylobacter jejuni nctc 11168 reveals the presence of orthologues of the chemotaxis genes chea, chew, chev, chey, cher and cheb, ten chemoreceptor genes and two aerotaxis genes. the presence of chev and a response regulator domain in chea, combined with the absence of a chez gene and the lack of a response regulator domain in cheb, reveals significant differences in the c. jejuni chemotaxis system compared with that found in other bacteria. | 2002 | 11912013 |
| inferring genome trees by using a filter to eliminate phylogenetically discordant sequences and a distance matrix based on mean normalized blastp scores. | darwin's paradigm holds that the diversity of present-day organisms has arisen via a process of genetic descent with modification, as on a bifurcating tree. evidence is accumulating that genes are sometimes transferred not along lineages but rather across lineages. to the extent that this is so, darwin's paradigm can apply only imperfectly to genomes, potentially complicating or perhaps undermining attempts to reconstruct historical relationships among genomes (i.e., a genome tree). whether most ... | 2002 | 11914337 |