Publications

the choice of the intravenous fluid influences the tolerance of acute normovolemic anemia in anesthetized domestic pigs.	the correction of hypovolemia with acellular fluids results in acute normovolemic anemia. whether the choice of the infusion fluid has an impact on the maintenance of oxygen (o₂) supply during acute normovolemic anemia has not been investigated so far.	2012	22546374
anomalous right coronary artery originating from the left sinus of valsalva in a yucatan minipig.	a 39.2-kg, castrated male yucatan minipig (sus scrofa domestica) was presented for enrollment in a coronary artery study. angiography revealed an anomalous right coronary artery originating from the left sinus of valsalva. the left anterior descending, left circumflex, and anomalous right coronary arteries were implanted with metallic stents without complications. the minipig remained on the study for 3 mo until it reached its predetermined study endpoint, during which time it showed no clinical ...	0	22546919
rat hepatitis e virus: geographical clustering within germany and serological detection in wild norway rats (rattus norvegicus).	zoonotic hepatitis e virus (hev) infection in industrialised countries is thought to be caused by transmission from wild boar, domestic pig and deer as reservoir hosts. the detection of hev-specific antibodies in rats and other rodents has suggested that these animals may represent an additional source for hev transmission to human. recently, a novel hev (rathev) was detected in norway rats from hamburg, germany, showing the typical genome organisation but a high nucleotide and amino acid sequen ...	2012	22554648
dead or alive: animal sampling during ebola hemorrhagic fever outbreaks in humans.	there are currently no widely accepted animal surveillance guidelines for human ebola hemorrhagic fever (ehf) outbreak investigations to identify potential sources of ebolavirus (ebov) spillover into humans and other animals. animal field surveillance during and following an outbreak has several purposes, from helping identify the specific animal source of a human case to guiding control activities by describing the spatial and temporal distribution of wild circulating ebov, informing public hea ...	2012	22558004
lymphocyte modulation with fty720 improves hemorrhagic shock survival in swine.	the inflammatory response to severe traumatic injury results in significant morbidity and mortality. lymphocytes have recently been identified as critical mediators of the early innate immune response to ischemia-reperfusion injury. experimental manipulation of lymphocytes following hemorrhagic shock may prevent secondary immunologic injury in surgical and trauma patients. the objective of this study is to evaluate the lymphocyte sequestration agent fty720 as an immunomodulator following experim ...	2012	22558085
high prevalence of toxoplasma gondii antibodies in domestic pigs in oaxaca state, mexico.	pigs are important in the epidemiology of toxoplasmosis in north america. seroprevalence of toxoplasma gondii infection in 525 domestic pigs (337 backyard raised, 188 farm raised) in oaxaca state, mexico was determined using the modified agglutination test (mat, cutoff 1:25). antibodies to t. gondii were found in 58 (17.2%) of the 337 backyard pigs with mat titers of 1:25 in 10, 1:50 in 12, 1:100 in 18, 1:200 in 6, 1:400 in 6, 1:800 in 3, and 1:1,600 in 3. seropositive pigs were found in 39 (37. ...	2012	22559725
experimental induction of malignant catarrhal fever in pigs with ovine herpesvirus 2 by intranasal nebulization.	malignant catarrhal fever (mcf), a frequently fatal herpesviral disease primarily of ruminant species, has been sporadically reported in pigs. all cases of naturally occurring porcine mcf reported to date have been linked to ovine herpesvirus 2 (ovhv-2), a gammaherpesvirus in the genus macavirus carried by sheep. experimental induction of mcf by aerosolization of the virus in nasal secretions collected from infected sheep has been successful in bison, cattle and rabbits. the goals of this study ...	2012	22560763
on-farm measurement of electrical conductivity for the estimation of ammonium nitrogen concentration in pig slurry.	pig (sus scrofa domesticus) slurry (ps) is commonly applied as fertilizer to agricultural fields. knowledge of ps nitrogen content is essential for good management, but ps nitrogen content is highly variable, not only between farms but also within a farm. laboratory analysis of animal slurries is often expensive and impractical for routine farmer use. therefore, when slurry is spread on land, its fertilizer value is generally unknown, resulting in the risk of pollution. in this work, two rapid a ...	2013	22565270
porcine teschovirus in wild boars in hungary.	the genus teschovirus, family picornaviridae, currently includes 12 serotypes (ptv 1 to 12) isolated from swine. ptvs have been well studied in domestic pigs, but knowledge about ptvs in wild boars is deficient. here, we report the first complete ptv genome sequence from 7 (70 %) of 10 fecal samples of wild boar piglets (sus scrofa) by rt-pcr and pyrosequencing. analysis of the wild boar ptv strain wb2c-tv/2011/hun (jq429405) showed considerable difference, especially in vp1 (66-74 % amino acid ...	2012	22569887
phylogeny and evolutionary genetics of porcine parvovirus in wild boars.	porcine parvovirus (ppv) is widespread among swine and is responsible for reproductive failure of susceptible sows, characterized by embryonic and fetal death. studies showed that ppv in domestic pig is genetically diverse and some strains differ from the ones used for vaccination. organ samples from wild boars and domestic pigs were collected in transylvania (romania) and tested for the presence of ppv by polymerase chain reaction. positive samples were grouped and 14 from the wild boar and 1 f ...	2012	22575819
the survey of porcine teschoviruses, sapeloviruses and enteroviruses b infecting domestic pigs and wild boars in the czech republic between 2005 and 2011.	this study presents results of epidemiological survey and genetic characterisation of porcine enteric picornaviruses belonging to the genera teschovirus, sapelovirus, and porcine enterovirus b. faecal or gut content samples from domestic pigs (sus scrofa f. domestica) and the cecal content of wild boars (sus scrofa) of different ages (collected between 2005 and 2011) were analysed by molecular methods. porcine enterovirus b was the most prevalent virus detected in both domestic pigs and wild boa ...	2012	22579481
molecular insights into the regulation of iron metabolism during the prenatal and early postnatal periods.	molecular iron metabolism and its regulation are least well understood in the fetal and early postnatal periods of mammalian ontogenic development. the scope of this review is to summarize recent progress in uncovering the molecular mechanisms of fetal iron homeostasis, introduce the molecules involved in iron transfer across the placenta, and briefly explain the role of iron transporters in the absorption of this microelement during early postnatal life. these issues are discussed and parallels ...	2012	22581367
detection and characterization of hepatitis e virus in domestic pigs of different ages in portugal.	this study represents the primary hepatitis e virus (hev) surveillance in domestic pigs in portugal, five pig farms were investigated in 5 different portuguese regions, ten faecal samples were collected at four different stages of the production. all faecal samples were tested for hepatitis e virus by real-time rt-pcr. at least one sample from each farms of all age groups tested positive for hev. the prevalence in the pig herds varied from 10% to 30% and the mean prevalence was 32% in weaners, 2 ...	2012	22583975
microvascular patterns in the blubber of shallow and deep diving odontocetes.	blubber, a specialized form of subdermal adipose tissue, surrounds marine mammal bodies. typically, adipose tissue is perfused by capillaries but information on blubber vascularization is lacking. this study's goals were to: 1) describe and compare the microvasculature (capillaries, microarterioles, and microvenules) of blubber across odontocete species; 2) compare microvasculature of blubber to adipose tissue; and 3) examine relationships between blubber's lipid composition and its microvascula ...	2012	22592863
antibodies to west nile virus and related flaviviruses in wild boar, red foxes and other mesomammals from spain.	red foxes (vulpes vulpes), wild boar (sus scrofa) and iberian pigs (sus scrofa domestica) that are raised extensively outdoors, as well as other wild mesomammals from south central spain and wild boar from doñana national park (dnp), were tested for antibodies against related flaviviruses by elisa and for antibodies against wnv by vnt. mean flavivirus seroprevalence according to elisa was 20.4 ± 7.8% (21 out of 103) in red foxes, 12.6 ± 2.8% (69 out of 545) in wild boars, and 3.3±2.7% (6 out of ...	2012	22595138
reduced aluminum contamination decreases parenteral nutrition associated liver injury.	parenteral nutrition-associated cholestasis remains a significant problem, especially for the surgical neonates. aluminum is a toxic element known to contaminate parenteral nutrition. we hypothesize that parenterally administered aluminum causes liver injury similar to that seen in parenteral nutrition-associated cholestasis.	2012	22595567
do pharmacokinetics explain persistent restenosis inhibition by a single dose of paclitaxel?	the purpose of this study was to investigate the elimination of paclitaxel from the arterial wall after a single short administration with a coated balloon.	2012	22619258
early superoxide scavenging accelerates renal microvascular rarefaction and damage in the stenotic kidney.	renal artery stenosis (ras), the main cause of chronic renovascular disease (rvd), is associated with significant oxidative stress. chronic rvd induces renal injury partly by promoting renal microvascular (mv) damage and blunting mv repair in the stenotic kidney. we tested the hypothesis that superoxide anion plays a pivotal role in mv dysfunction, reduction of mv density, and progression of renal injury in the stenotic kidney. ras was induced in 14 domestic pigs and observed for 6 wk. seven ras ...	2012	22622460
novel approach to therapeutic ercp after long-limb roux-en-y gastric bypass surgery using transgastric self-expandable metal stents: experimental outcomes and first human case study (with videos).	ercp in roux-en-y gastric bypass (rygb) patients is challenging. balloon-assisted enteroscopy (bae) allows access to the excluded stomach with creation of a percutaneous endoscopic gastrostomy (peg). transgastric self-expandable metal stent (sems) placement may allow antegrade ercp in 1 session.	2012	22624815
cadmium-induced changes in genomic dna-methylation status increase aneuploidy events in a pig robertsonian translocation model.	although cadmium is a well-established human carcinogen, the mechanisms by which it induces cancer are poorly understood. it is suggested that cadmium-mediated carcinogenesis may include the modulation of gene expression and signal-transduction pathways, interference with antioxidant enzymes, inhibition of dna repair and dna methylation, and induction of apoptosis. nevertheless, no predominant mechanism playing a role in metal-induced carcinogenesis has been reported. in the present study, we us ...	2012	22640880
an acute animal model of aortic stenosis: initial attempts at leaflet modification.	percutaneous valve insertion is an emerging treatment for aortic stenosis (as). to date, no large animal model exists that replicates human calcific as; moreover, the absence of any valve pathology in currently available animal models prevents their use in any realistic assessment of percutaneous aortic valve therapy. hence, the aim of the present study was to create an acute large animal model in which human calcific as could be simulated.	2012	22645851
epizootiology of trichinellosis in pigs and wild boars in western romania, 1998-2011.	this article reports for the first time data regarding trichinellosis in sacrificed animals (domestic pigs and wild boars) inspected during the period from january 1998 to november 2011 in the largest of the romanian counties. the data were collected from the veterinary public health department of timis county. a total of 5,586,431 domestic pigs on farms, 609,325 pigs in private households, and 823 wild boars were examined during the study period. the results indicated that 681 domestic pigs (0. ...	2012	22651387
combined intravenous treatment with ascorbic acid and desferrioxamine to reduce myocardial reperfusion injury in an experimental model resembling the clinical setting of primary pci.	during reperfusion of ischemic myocardium, oxygen-derived free radicals are produced and can cause deleterious effects, known as reperfusion injury. we aimed to determine if a combination of the antioxidant ascorbic acid and an iron-chelating agent desferrioxamine, which reduces the production of the hydroxyl radical via ferrum-catalyzed reactions, can exert a protective action against reperfusion injury.	2013	22653244
frequency and characterization of porcine hokovirus (phov) in domestic pigs in eastern china.	this study reports the frequency and age distribution of porcine hokovirus (phov) in domestic pigs and characterizes the strains prevalent in eastern china. a total of 242 tissues from domestic pigs were tested for phov using pcr, and six near-full-length genome sequences were used for phylogenetic analysis. the phov sequences from domestic pigs in china showed a close relationship to sequences of isolates from romania and hong kong. the results provide evidence that there are differences betwee ...	2012	22653537
evaluating the feasibility of direct peroral cholangioscopy training with an endoscopic simulator.	direct peroral cholangioscopy (dpocs) was reported to present clinical potential, and creating a target-specific training program for biliary endoscopists who lack experience with dpocs is an important task.	2012	22661276
ribozyme-mediated gene knock down strategy to dissect the consequences of pdgf stimulation in vascular smooth muscle cells.	vascular smooth muscle cells (vsmcs), due to their plasticity and ability to shift from a physiological contractile-quiescent phenotype to a pathological proliferating-activated status, play a central role in the onset and progression of atherosclerosis and cardiovascular diseases. pdgf-bb, among a series of cytokines and growth factors, has been identified as the critical factor in this phenotypic switch. in order to obtain new insights on the molecular effects triggered by pdgf-bb, a hammerhea ...	2012	22676333
the mammary gland-specific marsupial elp and eutherian cti share a common ancestral gene.	the marsupial early lactation protein (elp) gene is expressed in the mammary gland and the protein is secreted into milk during early lactation (phase 2a). mature elp shares approximately 55.4% similarity with the colostrum-specific bovine colostrum trypsin inhibitor (cti) protein. although elp and cti both have a single bovine pancreatic trypsin inhibitor (bpti)-kunitz domain and are secreted only during the early lactation phases, their evolutionary history is yet to be investigated.	2012	22681678
tnf-α blockade improves early post-resuscitation survival and hemodynamics in a swine model of ischemic ventricular fibrillation.	inflammatory cytokines have been implicated in the pathophysiology of post cardiac arrest syndrome, including myocardial dysfunction and hypotension, often leading to multi-organ system dysfunction and death. we hypothesized that administration of infliximab after return of spontaneous circulation (rosc) would ameliorate hypotension and myocardial dysfunction and prolong survival.	2013	22683946
current state of knowledge on porcine circovirus type 2-associated lesions.	porcine circovirus type 2 (pcv2), a small single-stranded dna virus, was initially discovered in 1998 and is highly prevalent in the domestic pig population. disease manifestations associated with pcv2 include postweaning multisystemic wasting disease (pmws), enteric disease, respiratory disease, porcine dermatitis and nephropathy syndrome (pdns), and reproductive failure. although these clinical manifestations involve different organ systems, there is considerable overlap in clinical expression ...	2013	22692624
new swine model of infected soft tissue blast injury.	war injuries, especially blast injuries, have a high risk of infection. however, no animal models of infected war injuries have been built in large animals, which retards both the understanding and the treatment optimization of infected war injuries.	2012	22710779
effects of intravenous sulfide during resuscitated porcine hemorrhagic shock*.	controversial data are available on the effects of hydrogen sulfide during hemorrhage. because the clinical significance of hydrogen sulfide administration in rodents may not be applicable to larger species, we tested the hypothesis whether intravenous na2s (sulfide) would beneficially influence organ dysfunction during long-term, porcine hemorrhage and resuscitation.	2012	22713217
enhanced characterization of the smell of death by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (gcxgc-tofms).	soon after death, the decay process of mammalian soft tissues begins and leads to the release of cadaveric volatile compounds in the surrounding environment. the study of postmortem decomposition products is an emerging field of study in forensic science. however, a better knowledge of the smell of death and its volatile constituents may have many applications in forensic sciences. domestic pigs are the most widely used human body analogues in forensic experiments, mainly due to ethical restrict ...	2012	22723918
proliferative vitreoretinopathy in the swine-a new model.	to develop a large animal model of proliferative vitreoretinopathy (pvr) in the swine to eventually study disease pathophysiology, as well as novel therapies.	2012	22729438
genome-wide single nucleotide polymorphism analysis reveals recent genetic introgression from domestic pigs into northwest european wild boar populations.	present-day genetic introgression from domestic pigs into european wild boar has been suggested in various studies. however, no hybrids have been identified beyond doubt mainly because available methods were unable to quantify the extent of introgression and rule out natural processes. genetic introgression from domestic pigs may have far-reaching ecological consequences by altering traits like the reproduction rate or immunology of wild boar. in this study, we demonstrate a novel approach to in ...	2013	22731769
endovascular broad-neck aneurysm creation in a porcine model using a vascular plug.	ruptured cerebral arterial aneurysms require prompt treatment by either surgical clipping or endovascular coiling. training for these sophisticated endovascular procedures is essential and ideally performed in animals before their use in humans. simulators and established animal models have shown drawbacks with respect to degree of reality, size of the animal model and aneurysm, or time and effort needed for aneurysm creation. we therefore aimed to establish a realistic and readily available ane ...	2013	22735890
prevalence of infection and genetic diversity of porcine circovirus type 2 (pcv2) in wild boar (sus scrofa) in poland.	porcine circovirus type 2 (pcv2) is a widespread, important pathogen of domestic swine and the causative agent of postweaning multisystemic wasting syndrome and other diseases and conditions referred to as "porcine circovirus diseases." specific antibodies and dna to pcv2 have also been detected in european wild boars and north american feral pigs. we collected 312 tonsil samples from wild boars shot in 13 of 16 districts of poland, and tested them for pcv2 dna using a real-time pcr. we detected ...	2012	22740527
[pure transgastric notes laparoscopy compared to classic laparoscopy].	notes--natural orifice transluminal endoscopic surgery--represents a new surgical approach. the operation is performed via natural openings of the body without causing any damage to the skin and the abdominal wall. at the department of surgery of the university hospital in pilsen, we have been performing notes since 2008. the first animal experiment was performed in that year by a hybrid notes technique.	2012	22746085
heparin does not improve graft function in uncontrolled non-heart-beating lung donation: an experimental study in pigs.	non-heart-beating donation (nhbd) has the potential to increase the number of patients treated with lung transplantation. our study investigated, in a simulated clinical situation in the uncontrolled nhbd setting, whether or not heparin administration after death affects the donor lung function.	2013	22761497
seroprevalence of toxoplasma gondii in domestic pigs (sus scrofa domestica) and wild boars (sus scrofa) in latvia.	consumption of undercooked pork products is considered a major risk factor for contracting toxoplasmosis in humans in several countries. in total, 803 pork samples and 606 wild boar meat samples were collected from different regions of latvia during june 2010 and february 2011 were tested for toxoplasma gondii antibodies using an in-house elisa assay. seroprevalence in wild boar (33.2%, p < 0.05) was significantly higher than in domestic pig (4.2%). the prevalence of t. gondii -specific antibodi ...	2013	22765652
microvascularization on collared peccary placenta: a microvascular cast study [corrected] in late pregnancy.	the microvascularization of the collared peccary (tayassu tajacu) placenta was studied by vascular casts and immunolocalization of α-smooth muscle actin and vimentin, to identify the three dimensional organization and vascular flow interrelation in the microvasculature between the maternal and fetal compartments of the placentae. the immunolocalization of vimentin in the vascular endothelium and in the smooth muscle cells of blood vessels showed indented capillaries along the uterine epithelium ...	2012	22775252
development of a suspension microarray for the genotyping of african swine fever virus targeting the snps in the c-terminal end of the p72 gene region of the genome.	african swine fever virus (asfv) causes one of the most dreaded transboundary animal diseases (tads) in suidae. african swine fever (asf) often causes high rates of morbidity and mortality, which can reach 100% in domestic swine. to date, serological diagnosis has the drawback of not being able to differentiate variants of this virus. previous studies have identified the 22 genotypes based on sequence variation in the c-terminal region of the p72 gene, which has become the standard for categoriz ...	2013	22776009
use of ¹⁸f-fluorodeoxyglucose positron emission tomography-computed tomography in a miniature pig (sus scrofa domestica) with pneumonia.	a 1-y-old male miniature pig housed in our laboratory facility was evaluated for weight loss and rough coat condition. cbc results revealed neutrophilia. radiography of the thoracic area showed increased opacity throughout the thoracic cavity except for the right caudal lobe. ¹⁸f-labeled fluorodeoxyglucose positron emission tomography-computed tomography (fdg-pet-ct) revealed elevated standard uptake values in the area corresponding to the radiologic findings. follow-up thoracic radiography take ...	0	22776053
effects of various anesthetic protocols on 18f-flurodeoxyglucose uptake into the brains and hearts of normal miniature pigs (sus scrofa domestica).	this study used positron emission tomography-computed tomography (pet-ct) to evaluate the effects of 4 anesthetic protocols on 2-deoxy-2-[18f]-fluoro-d-glucose (18f-fdg) accumulation in the brains and hearts of miniature pigs (sus scrofa domestica). the 18f-fdg standard uptake value was quantified by dividing the brain into 6 regions: cerebellum, brainstem, and frontal, parietal, temporal, and occipital lobes. five (2 female and 3 male) clinically normal miniature pigs were premedicated with med ...	0	22776126
brain growth of the domestic pig (sus scrofa) from 2 to 24 weeks of age: a longitudinal mri study.	an animal model with brain growth similar to humans, that can be used in mri studies to investigate brain development, would be valuable. our laboratory has developed and validated mri methods for regional brain volume quantification in the neonatal piglet. the aim of this study was to utilize the mri-based volume quantification technique in a longitudinal study to determine brain growth in domestic pigs from 2 to 24 weeks of age. mri data were acquired from pigs 2-24 weeks of age using a 3-dime ...	2012	22777003
vasomotor response to different endothelium-dependent vasodilators in an animal model.	incomplete re-endothelialization of stents can be revealed as paradoxical vasoconstriction with endothelium-dependent vasodilators. as no consensus exists about the best method or agent, our objective is to analyze the response to different drugs in a coronary swine model.	2012	22781469
diagnosis of mycobacterium avium complex in granulomatous lymphadenitis in slaughtered domestic pigs.	the aim of this study was to compare two diagnostic methods for the detection of mycobacterium avium complex (mac) infection in lymph nodes with granulomatous lymphadenitis from slaughtered domestic pigs. fifty affected lymph nodes were collected from 50 pigs and examined microscopically and by polymerase chain reaction (pcr). microscopically, granulomatous lesions were observed in 92% of the samples, consisting mostly of central necrosis (78%) with dystrophic calcification (46%) and associated ...	2012	22784782
nucleotide variability and linkage disequilibrium patterns in the porcine muc4 gene.	muc4 is a type of membrane anchored glycoprotein and serves as the major constituent of mucus that covers epithelial surfaces of many tissues such as trachea, colon and cervix. muc4 plays important roles in the lubrication and protection of the surface epithelium, cell proliferation and differentiation, immune response, cell adhesion and cancer development. to gain insights into the evolution of the porcine muc4 gene, we surveyed the nucleotide variability and linkage disequilibrium (ld) within ...	2012	22793500
ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular injury in a porcine model.	high restenosis rates are a limitation of peripheral vascular interventions. previous studies have shown that surgical implantation of a tissue-engineered endothelium onto the adventitia surface of injured vessels regulates vascular repair. in the present study, we developed a particulate formulation of tissue-engineered endothelium and a method to deliver the formulation perivascular to injured blood vessels using a percutaneous, minimally invasive technique.	2012	22796118
antegrade in situ fenestration of aortic stent graft: in-vivo experiments using a pig model.	short proximal neck of an abdominal aortic aneurysm is associated with risk of treatment failure during abdominal aortic repair. important side branches, such as renal arteries, cannot be covered without serious consequences.	2012	22798292
time course of endothelium-dependent and -independent coronary vasomotor response to coronary balloons and stents. comparison of plain and drug-eluting balloons and stents.	this study sought to determine the time dependency of the endothelium-dependent and -independent vascular responses after percutaneous coronary intervention (pci) with drug-eluting (deb) or plain balloons, bare-metal (bms), and drug-eluting (des) stents, or controls.	2012	22814779
conserved synthetic peptides from the hemagglutinin of influenza viruses induce broad humoral and t-cell responses in a pig model.	outbreaks involving either h5n1 or h1n1 influenza viruses (iv) have recently become an increasing threat to cause potential pandemics. pigs have an important role in this aspect. as reflected in the 2009 human h1n1 pandemia, they may act as a vehicle for mixing and generating new assortments of viruses potentially pathogenic to animals and humans. lack of universal vaccines against the highly variable influenza virus forces scientists to continuously design vaccines à la carte, which is an expen ...	2012	22815759
serological evidence of ebola virus infection in indonesian orangutans.	ebola virus (ebov) and marburg virus (marv) belong to the family filoviridae and cause severe hemorrhagic fever in humans and nonhuman primates. despite the discovery of ebov (reston virus) in nonhuman primates and domestic pigs in the philippines and the serological evidence for its infection of humans and fruit bats, information on the reservoirs and potential amplifying hosts for filoviruses in asia is lacking. in this study, serum samples collected from 353 healthy bornean orangutans (pongo ...	2012	22815803
risk factors for contacts between wild boar and outdoor pigs in switzerland and investigations on potential brucella suis spill-over.	due to the parallel increase of the number of free-ranging wild boar and domestic pigs reared outdoor, the risk that they interact has become higher. contacts with wild boar can be the origin of disease outbreaks in pigs, as it has been documented for brucellosis in some european countries. this study aimed at quantifying the occurrence of contacts between wild boar and outdoor domestic pigs in switzerland, and identifying risk factors for these contacts. furthermore, exposed pigs were tested fo ...	2012	22817843
myocardium at risk can be determined by ex vivo t2-weighted magnetic resonance imaging even in the presence of gadolinium: comparison to myocardial perfusion single photon emission computed tomography.	determination of the myocardium at risk (mar) and final infarct size by cardiac magnetic resonance imaging (cmr) enables calculation of salvaged myocardium in acute infarction. t2-weighted imaging is performed prior to the administration of gadolinium, since gadolinium affects t2 tissue properties. this is, however, difficult in an ex vivo model since gadolinium must be administered for determination of infarct size by cmr. we aimed to test the ability of ex vivo t2-weighted imaging to assess ma ...	2013	22822155
vascular response and mechanical integrity of the new biodegradable polymer coated sirolimus-eluting prolim stent implanted in porcine coronary arteries.	although durable polymer coated drug-eluting stents (des) are standard care in percutaneous coronary interventions, new stent platforms employing biodegradable polymer based drug delivery are increasingly being used in clinical practice.	2012	22825946
oral immunization of wild boar and domestic pigs with attenuated live vaccine protects against pseudorabies virus infection.	in domestic pigs strict control measures and the use of gene-deleted marker vaccines resulted in the elimination of pseudorabies virus (prv) infections in many parts of europe and north america. in free-roaming feral pigs and wild boar populations, however, serological surveys and monitoring in the americas, europe and north africa provided serological and virological evidence that prv is more widely distributed than previously assumed. thus, there is a constant risk of spillover of prv infectio ...	2012	22832373
submucosal endoscopy with mucosal resection: a hybrid endoscopic submucosal dissection in the porcine rectum and distal colon.	endoscopic submucosal dissection (esd) has appeal for en bloc resection of large flat colorectal polyps but carries appreciable risk and demands a high level of expertise and training. undermining flat and laterally spreading colorectal polyps by using submucosal endoscopy with the mucosal safety valve flap (semf) may be combined with esd to offer a hybrid technique that is easier and safer.	2012	22854058
analyses of intestinal commensal escherichia coli strains from wild boars suggest adaptation to conventional pig production conditions.	to test the hypothesis that escherichia coli populations have adapted to conventional pig production practices, we comparatively tested intestinal commensal e. coli from wild boars versus isolates from domestic pigs by analyzing virulence-associated factors, adhesion, and metabolic activities. virulence-associated genes typical for intestinal pathogenic e. coli (invags) were sporadically detected among e. coli from wild boars except the adhesion-related gene paa and the enterotoxin-encoding gene ...	2012	22857976
development of a porcine (sus scofa) embryo-specific microarray: array annotation and validation.	the domestic pig is an important livestock species and there is strong interest in the factors that affect the development of viable embryos and offspring in this species. a limited understanding of the molecular mechanisms involved in early embryonic development has inhibited our ability to fully elucidate these factors. next generation deep sequencing and microarray technologies are powerful tools for delineation of molecular pathways involved in the developing embryo.	2012	22863022
complete genome sequence of an avian-like h4n8 swine influenza virus discovered in southern china.	we report here the complete genomic sequence of an avian-like h4n8 swine influenza virus containing an h5n1 avian influenza virus segment from swine in southern china. phylogenetic analyses of the sequences of all eight viral rna segments demonstrated that these are wholly avian influenza viruses of the asia lineage. to our knowledge, this is the first report of interspecies transmission of an avian h4n8 influenza virus to domestic pigs under natural conditions.	2012	22879613
[outbreaks of viral hepatitis e in the czech republic?].	until recently, viral hepatitis e (vhe) has typically been an imported infection, related to travel to developing countries. a number of travel-unrelated vhe cases currently diagnosed in the czech republic. outcomes of the epidemiological investigations of two vhe outbreaks associated with the consumption of pork and pork products at pig-slaughtering feasts are presented. thirteen cases have been reported in the first outbreak and eight cases in the second outbreak. the epidemiological investiga ...	2012	22880260
the attraction of virgin female hide beetles (dermestes maculatus) to cadavers by a combination of decomposition odour and male sex pheromones.		2012	22889339
[not available].		0	22896144
identification of the x chromosome of the domestic pig (sus scrofa domestica).		0	22896166
centromeric heterochromation exchange by an autosomal reciprocal 1/16 translocation in the pig (sus scrofa domestica).		0	22896238
identification of the chromosomes of the domestic pig (sus scrofa domestica). an identification key and a landmark system.		0	22896553
two newly developed e(rns)-based elisas allow the differentiation of classical swine fever virus-infected from marker-vaccinated animals and the discrimination of pestivirus antibodies.	new generations of classical swine fever virus (csfv) marker vaccines have recently been developed in order to make emergency vaccination in case of a csf outbreak more feasible. however, the application of a marker vaccine is dependent on the availability of an accompanying discriminatory test allowing differentiation of infected from vaccinated animals (diva). cp7_e2alf, the most promising live marker vaccine candidate currently available, is a genetically modified bovine viral diarrhea virus ...	2013	22902189
study of the genetic variability of porcine circovirus type 2 detected in serbia and slovenia.	recent variants of porcine circovirus type 2 (pcv2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from serbia and slovenia. a 450-base-pair nucleotide sequence was obtained by pcr from the orf2. the derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related pcv2 sequences determined in previous years and retrieved from the genbank. the 30 serbian and 17 ...	2012	22903085
[the coastal areas of yantai human and swine hepatitis e virus genotyping analysis].	survey of the coastal city of yantai, from human and swine hepatitis e virus (hev) genotype correlation.	2012	22919748
first detection of hepatitis e virus in austrian pigs by rt-qpcr.	long known to cause disease outbreaks in man in countries with poor sanitary conditions, an increasing number of autochthonous hev genotype 3 infections have been reported in industrialised countries. genotype 3 poses an important potential zoonotic threat, with infected pigs functioning as the main reservoir. this study reports the first detected emergence of hev in austrian pigs. five austrian strains were partially sequenced and phylogenetic analysis demonstrates that they cluster within geno ...	2012	22919921
trichinella patagoniensis n. sp. (nematoda), a new encapsulated species infecting carnivorous mammals in south america.	until a few years ago, trichinella spiralis was the only taxon of the genus trichinella detected in both domestic and wild animals of south america. recently, a new genotype, named trichinella t12, was identified in cougars (puma concolor) from argentina, on the basis of molecular studies using mitochondrial and nuclear ribosomal markers. in the present study, cross-breeding experiments indicated that trichinella t12 is reproductively isolated from all other encapsulated trichinella spp. and sug ...	2012	22921601
nad(p)h cytochrome b5 oxidoreductase deficiency in leishmania major results in impaired linoleate synthesis followed by increased oxidative stress and cell death.	nad(p)h cytochrome b(5) oxidoreductase (ncb5or), comprising cytochrome b(5) and cytochrome b(5) reductase domains, is widely distributed in eukaryotic organisms. although ncb5or plays a crucial role in lipid metabolism of mice, so far no ncb5or gene has been reported in the unicellular parasitic protozoa leishmania species. we have cloned, expressed, and characterized ncb5or gene from leishmania major. steady state catalysis and spectral studies show that nadh can quickly reduce the ferric state ...	2012	22923617
epidemiology, clinical, immune, and molecular profiles of microsporidiosis and cryptosporidiosis among hiv/aids patients.	the objective of this study was to determine the prevalence of intestinal parasites, with special emphasis on microsporidia and cryptosporidium, as well as their association with human immunodeficiency virus (hiv) symptoms, risk factors, and other digestive parasites. we also wish to determine the molecular biology definitions of the species and genotypes of microsporidia and cryptosporidium in hiv patients.	2012	22924007
genetic characterization of swine influenza viruses isolated in japan between 2009 and 2012.	eleven swine influenza viruses (sivs) isolated from pigs in japanese institutions between 2009 and 2012 were genetically characterized. seven h1n1 were shown to have originated from a(h1n1)pdm09 viruses. two h1n2 viruses contained h1 and n2 genes of japanese h1n2 siv origin together with internal genes of a(h1n1)pdm09 viruses. two h3n2 viruses isolated during animal quarantine were identified as triple reassortant h3n2 viruses maintained among pigs in north america. this study shows that a(h1n1) ...	2012	22924526
prevalence of classical swine fever virus in domestic pigs in south korea: 1999-2011.	the major policy for eradication of classical swine fever (csf) in south korea has focused on the implementation of compulsory vaccination of the susceptible pig population. a vaccine strain of csf virus, the lom strain, is used to maintain high herd seroconversion, a practice complementary to the 'stamping-out policy' and restriction of animal movement during disease outbreaks. to survey for the prevalence of csf in domestic pigs in south korea over the past 13 years (1999-2011), we tested 4 19 ...	2013	22925439
porcine kobuvirus in wild boars (sus scrofa).	fecal samples (n = 10) from 6- to 8-week-old wild boar piglets (sus scrofa), collected from an animal park in hungary in april 2011, were analyzed using viral metagenomics and complete genome sequencing. kobuvirus (genus kobuvirus, family picornaviridae) was detected in all (100 %) specimens, with the closest nucleotide (89 %) and amino acid (94 %) sequence identity of the strain wild boar/wb1-hun/2011/hun (jx177612) to the prototype porcine kobuvirus s-1-hun (eu787450). this study suggests that ...	2012	22926717
infrahepatic caudal/inferior vena cava interruption with azygos/hemiazygos continuation. vascular anomaly in swine.	swine are commonly used as a model to study congenital cardiovascular defects that occur in humans and these models have been both spontaneous and experimentally induced. ventricular septal defect, patent ductus arteriosus, and atrial septal defect (asd) are examples of experimentally induced models. absence of caudal/inferior vena cava (cvc/ivc) with azygos/hemiazygos continuation is an uncommon vascular anomaly.	2010	22933907
effects of local delivery of tetradecylthioacetic acid within the injured coronary vessel wall.	inflammation is involved in cell proliferation and collagen deposition causing vessel wall remodeling and restenosis after plain balloon angioplasty. local drug delivery of bioactive agents that reduce the incidence of adverse wall remodeling is of considerable interest concerning treatment strategies for coronary vessel disease and could alter the need of repeated revascularization.	2012	22934973
identification of chromosomal locations associated with tail biting and being a victim of tail-biting behaviour in the domestic pig (sus scrofa domesticus).	the objective of this study was to identify loci associated with tail biting or being a victim of tail biting in norwegian crossbred pigs using a genome-wide association study with plink case-control analysis. dna was extracted from hair or blood samples collected from 98 trios of crossbred pigs located across norway. each trio came from the same pen and consisted of one pig observed to initiate tail biting, one pig which was the victim of tail biting and a control pig which was not involved in ...	2012	22941514
microsatellite markers for identification and parentage analysis in the european wild boar (sus scrofa).	the wild boar (sus scrofa) is among the most widespread mammal species throughout the old world. presently, studies concerning microsatellites in domestic pigs and wild boars have been carried out in order to investigate domestication, social behavior and general diversity patterns among either populations or breeds. the purpose of the current study is to develop a robust set of microsatellites markers for parentage analyses and individual identification.	2012	22943565
theria-specific homeodomain and cis-regulatory element evolution of the dlx3-4 bigene cluster in 12 different mammalian species.	the mammalian dlx3 and dlx4 genes are configured as a bigene cluster, and their respective expression patterns are controlled temporally and spatially by cis-elements that largely reside within the intergenic region of the cluster. previous work revealed that there are conspicuously conserved elements within the intergenic region of the dlx3-4 bigene clusters of mouse and human. in this paper we have extended these analyses to include 12 additional mammalian taxa (including a marsupial and a mon ...	2012	22951979
altered expression of porcine piwi genes and pirna during development.	three sus scrofa piwi genes (piwil1, piwil2 and piwil4) encoding proteins of 861, 985 and 853 aminoacids, respectively, were cloned and sequenced. alignment of the piwi proteins showed the high identity between sus scrofa and homo sapiens. relative transcript abundance of porcine piwil1, piwil2 and piwil4 genes in testes, ovaries and oocytes derived from sexually immature and mature animals was examined using real-time pcr. expression of the three piwi mrnas was proved to be tissue specific and ...	2012	22952772
phylogenetic evidence that two distinct trichuris genotypes infect both humans and non-human primates.	although there has been extensive debate about whether trichuris suis and trichuris trichiura are separate species, only one species of the whipworm t. trichiura has been considered to infect humans and non-human primates. in order to investigate potential cross infection of trichuris sp. between baboons and humans in the cape peninsula, south africa, we sequenced the its1-5.8s-its2 region of adult trichuris sp. worms isolated from five baboons from three different troops, namely the cape penins ...	2012	22952922
a t-cell epitope on ns3 non-structural protein enhances the b and t cell responses elicited by dendrimeric constructions against csfv in domestic pigs.	it has been recently reported by our group that dendrimeric constructs combining b- and t-cell epitopes from classical swine fever virus (csfv) provided partial protection against experimental infection. this research evaluated four newly designed constructions while taking into account our previous work, including the direct implication that a t-cell epitope from the ns3 protein contributes to the generation of the immune response against csfv. to this end, the dendrimeric constructions, includ ...	2012	22959286
descriptive and social network analysis of pig transport data recorded by quality assured pig farms in the uk.	the aim of this study was to examine farm-to-farm pig movement connections, and the usage of hauliers and abattoirs, for farms in the united kingdom (uk), to determine the interconnectivity of the pig farm network and the implications this may have for the transmission and control of salmonella, which was chosen as an example of an important endemic disease. data were collected from three quality assurance schemes on the use of abattoirs and livestock hauliers by commercial pig farms, and the su ...	2013	22959427
community dynamics of carrion flies and their parasitoids in experimental carcasses in central argentina.	insects are the predominant group regarding both species richness and abundance that develop on carrion. among them, the most important decomposers using carrion as a source of food for their development are the immature stages of the dipteran families calliphoridae, muscidae, and sarcophagidae. the large numbers of their larvae in carcasses are attacked by a rich community of parasitoids, including species of braconidae, ichneumonidae, and pteromalidae (hymenoptera: parasitica). the objective o ...	0	22963038
in vivo decontamination of the nerve agent vx using the domestic swine model.	the purpose of this in vivo study was to assess a new, putatively optimised method for mass casualty decontamination ("orchids protocol") for effectiveness in removing the chemical warfare agent vx from the skin of anaesthetised, domestic white pigs. orchids protocol consists of a 1.5-minute shower with a mild detergent (argos™) supplemented by physical removal. a standard method of wet decontamination was used for comparison. experimental animals were divided into four groups (a-d). two groups ...	2012	22963275
phylogenetic analysis of porcine astrovirus in domestic pigs and wild boars in south korea.	porcine astrovirus (pastv) belongs to genetically divergent lineages within the genus mamastrovirus. in this study, 25/129 (19.4 %) domestic pig and 1/146 (0.7 %) wild boar fecal samples tested in south korea were positive for pastv. positive samples were mainly from pigs under 6 weeks old. bayesian inference (bi) tree analysis for rna-dependent rna polymerase (rdrp) and capsid (orf2) gene sequences, including mamastrovirus and avastrovirus, revealed a relatively geographically divergent lineage ...	2013	22965450
fate and transformation of an estrogen conjugate and its metabolites in agricultural soils.	in the environment, conjugated estrogens are nontoxic but may hydrolyze to their potent unconjugated, 'free' forms. compared to free estrogens, conjugated estrogens would be more mobile in the environment because of their higher water solubility. to identify the fate of a conjugated estrogen in natural agricultural soils, batch experiments were conducted with a (14)c labeled prototype conjugate, 17β-estradiol-3-glucuronide (e2-3g). initially, aqueous dissipation was dominated by biological hydro ...	2012	22967238
cloning and expression of porcine colony stimulating factor-1 (csf-1) and colony stimulating factor-1 receptor (csf-1r) and analysis of the species specificity of stimulation by csf-1 and interleukin 34.	macrophage colony stimulating factor (csf-1) controls the survival, differentiation and proliferation of cells of the mononuclear phagocyte system. a second ligand for the csf-1r, interleukin 34 (il-34), has been described, but its physiological role is not yet known. the domestic pig provides an alternative to traditional rodent models for evaluating potential therapeutic applications of csf-1r agonists and antagonists. to enable such studies, we cloned and expressed active pig csf-1. to provid ...	2012	22974529
percutaneous transgastric traction-assisted esophageal endoscopic submucosal dissection: a randomized controlled trial in a porcine model.	endoscopic submucosal dissection (esd) is a technically difficult, hazardous, and time-consuming treatment option for superficial neoplasms of the esophagus. the aim of this pilot study was to determine the effectiveness and safety of percutaneous transgastric traction (ptt)-assisted esd, a method that overcomes the drawbacks of conventional esd, in a porcine model.	2012	22989307
derivation and characterization of sleeping beauty transposon-mediated porcine induced pluripotent stem cells.	the domestic pig is an important large animal model for preclinical testing of novel cell therapies. recently, we produced pluripotency reporter pigs in which the oct4 promoter drives expression of the enhanced green fluorescent protein (egfp). here, we reprogrammed oct4-egfp fibroblasts employing the nonviral sleeping beauty transposon system to deliver the reprogramming factors oct4, sox2, klf4, and cmyc. successful reprogramming to a pluripotent state was indicated by changes in cell morpholo ...	2013	22989381
importance of atrial surface area and refractory period in sustaining atrial fibrillation: testing the critical mass hypothesis.	the critical mass hypothesis for atrial fibrillation (af) was proposed in 1914; however, there have been few studies defining the relationship between atrial surface area and af. this study evaluated the effect of tissue area and effective refractory period (erp) on the probability of sustaining af in an in vivo model.	2013	22995722
reston virus in domestic pigs in china.	historically, reston virus (restv) has been found to be associated with outbreaks of disease only in nonhuman primates. its spread to domestic pigs was reported for the first time in 2008. in this study, we report the discovery, molecular detection, and phylogenetic analysis of reston virus (restv) in domestic pigs in china. a total of 137 spleen specimens from pigs that died after showing typical clinical signs of porcine reproductive and respiratory syndrome (prrs), and for which infection wit ...	2014	22996641
tissue distribution of clonidine following intraforaminal implantation of biodegradable pellets: potential alternative to epidural steroid for radiculopathy.	epidural steroid injections have shown efficacy in short-term pain relief, but often require repeated injections in order to provide continued pain relief. it has been suggested that a continuous, locally administered dose of an anti-inflammatory compound may provide sustained pain relief at doses lower than those needed with injections.	2014	22996864
minipig urethra: a suitable animal model in vitro.	the study demonstrates that the minipig urethra is an appropriate animal model for in vitro experiments and therefore promising for long-term animal tests of artificial urinary sphincter systems in vivo. freshly explanted porcine and minipig urethras were connected to a fluid reservoir that simulated the bladder pressure. this bladder pressure was adjusted by changing the water level (hydrostatic pressure). specially designed aluminum sphincters loaded with a fluid-filled container were used to ...	2012	23000561
viral metagenomics demonstrates that domestic pigs are a potential reservoir for ndumu virus.	the rising demand for pork has resulted in a massive expansion of pig production in uganda. this has resulted in increased contact between humans and pigs. pigs can act as reservoirs for emerging infectious diseases. therefore identification of potential zoonotic pathogens is important for public health surveillance. in this study, during a routine general surveillance for african swine fever, domestic pigs from uganda were screened for the presence of rna and dna viruses using a high-throughput ...	2012	23006778
preparative isolation and purification of three glycine-conjugated cholic acids from pulvis fellis suis by high-speed countercurrent chromatography coupled with elsd detection.	coupled with evaporative light scattering detection, a high-speed counter-current chromatography (hsccc) method was developed for preparative isolation and purification of three glycine-conjugated cholic acids, glycochenodeoxycholic acid (gcdca), glycohyodeoxycholic acid (ghdca) and glycohyocholic acid (ghca) from pulvis fellis suis (pig gallbladder bile) for the first time. the separation was performed with a two-phase solvent system consisted of chloroform-methanol-water-acetic acid (65:30:10: ...	2012	23008527
lymphocyte depletion in experimental hemorrhagic shock in swine.		2012	23009382
[sequence of depolarization of atrial myocardium of the pig (sus scrofa domesticus)].	by the method of multichannel synchronous cardioelectropotography, sequence of depolarization of intramural atrial layers was studied in representatives of ungulate animals--pigs. in the pig atrial myocardium there is revealed a complex picture of spreading of activation fronts, connected with non-uniform anatomical structure and subendocardial (intramural) disposition of area of initial activation. the general character of sequence of depolarization of the pig atrial myocardium is comparable wi ...	2012	23013027