Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| schistosoma japonicum soluble egg antigens facilitate hepatic stellate cell apoptosis by downregulating akt expression and upregulating p53 and dr5 expression. | the induction of hepatic stellate cell (hsc) apoptosis has potential as a potent strategy to diminish the progression of liver fibrosis. previous studies have demonstrated the ability of soluble egg antigens (sea) from schistosomes to inhibit hsc activation and to induce apoptosis in vitro. in this study, we aimed to explore the mechanism of sea-induced apoptosis in hscs. | 2014 | 25144704 |
| mapkap1 rs10118570 polymorphism is associated with anti-infection and anti-hepatic fibrogenesis in schistosomiasis japonica. | chronic infection with schistosoma japonicum is an important cause of hepatic fibrosis (hf). human 9q33.3 is one of the most important loci for stress-related diseases. we examined the potential associations of 43 single-nucleotide polymorphisms (snps) with s. japonicum infection and hf in epidemic region in china. we identified a snp (rs10118570 gg in mitogen-activated protein kinase associated protein 1, mapkap1) contributes to anti-infection (adjusted or = 0.35) and anti-fibrogenesis (adjuste ... | 2014 | 25153992 |
| genome-wide transcriptome analysis shows extensive alternative rna splicing in the zoonotic parasite schistosoma japonicum. | schistosoma japonicum is a pathogen of the phylum platyhelminthes that causes zoonotic schistosomiasis in china and southeast asian countries where a lack of efficient measures has hampered disease control. the development of tools for diagnosis of acute and chronic infection and for novel antiparasite reagents relies on understanding the biological mechanisms that the parasite exploits. | 2014 | 25156522 |
| linalool, derived from cinnamomum camphora (l.) presl leaf extracts, possesses molluscicidal activity against oncomelania hupensis and inhibits infection of schistosoma japonicum. | schistosomiasis japonicum remains a considerable economic and public health concern in china, the philippines and indonesia. currently available measures to control the unique intermediate host oncomelania hupensis are frequently associated with severe side effects. previous studies have demonstrated that linalool-rich extracts from various plants exhibited promising biological activities including cytotoxic, anti-microbial and anti-parasitic properties. | 2014 | 25174934 |
| wetlands explain most in the genetic divergence pattern of oncomelania hupensis. | understanding the divergence patterns of hosts could shed lights on the prediction of their parasite transmission. no effort has been devoted to understand the drivers of genetic divergence pattern of oncomelania hupensis, the only intermediate host of schistosoma japonicum. based on a compilation of two o. hupensis gene datasets covering a wide geographic range in china and an array of geographical distance and environmental dissimilarity metrics built from earth observation data and ecological ... | 2014 | 25183028 |
| evaluation of protective immune response in mice by vaccination the recombinant adenovirus for expressing schistosoma japonicum inhibitor apoptosis protein. | schistosomiasis is a worldwide parasitic disease, and while it can be successfully treated with chemotherapy, this does not prevent reinfection with the parasite. adenovirus vectors have been widely used for vaccine delivery, and a vaccination approach has the potential to prevent infection with schistosoma. here, we developed a recombinant adenoviral vector that expresses schistosoma japonicum inhibitor apoptosis protein (ad-sjiap) and assessed its immunoprotective functions against schistosomi ... | 2014 | 25185668 |
| compatibility of schistosoma japonicum from the hilly region and oncomelania hupensis hupensis from the marshland region within anhui, china. | schistosome japonicum remains one main public concern in china. this is exemplified in the hilly region in anhui province, where rodents have served as reservoirs for the parasite and no effective intervention could target such wild animals. the closer relationship between the hilly region and the near marshland induces the worry of spread of the hill parasite to the marshland region. therefore, the level of snail-parasite compatibility between the hill parasite and snail populations from the ya ... | 2014 | 25199558 |
| [cloning and characterization of conservative region of tyrosine kinase 4, a novel gender-associated gene of schistosoma japonicum]. | to clone and express the conservative region of gene encoding tyrosine kinase 4 of schistosoma japonicum and identify the difference in gene expression between genders of s. japonicum. | 2014 | 25223047 |
| [effect of toll-like receptor (tlr) 7 deficiencies on the in vivo immune response against schistosoma japonicum]. | to explore the toll-like receptor 7 knocked out (tlr7-/-) mice immune response against schistosoma japonicum. | 2014 | 25223048 |
| [in vitro effect of photoactivated hypericin on anti-schistosoma japonicum adult male worms]. | to investigate the in vitro effect of photoactivated hypericin on anti-schistosoma japonicum adult male worms. | 2014 | 25223049 |
| evaluation of banked urine samples for the detection of circulating anodic and cathodic antigens in schistosoma mekongi and s. japonicum infections: a proof-of-concept study. | in asia, schistosoma japonicum is the predominant schistosome species, while schistosoma mekongi is confined to limited foci in cambodia and lao people's democratic republic. while the people's republic of china has been successful in controlling schistosomiasis, the disease remains a major public health issue in other areas. in order to prioritise intervention areas, not only accurate diagnosis is important but also other factors, such as practicality, time-efficiency and cost-effectiveness, si ... | 2015 | 25225158 |
| schistosoma japonicum in samar, the philippines: infection in dogs and rats as a possible risk factor for human infection. | the role that animals play in the transmission of schistosoma japonicum to humans in the philippines remains uncertain and prior studies have not included several species, adjustment for misclassification error and clustering, or used a cohort design. a cohort study of 2468 people providing stool samples at 12 months following praziquantel treatment in 50 villages of western samar, the philippines, was conducted. stool samples from dogs, cats, rats, and water buffaloes were collected at baseline ... | 2015 | 25274409 |
| expression profile of the schistosoma japonicum degradome reveals differential protease expression patterns and potential anti-schistosomal intervention targets. | blood fluke proteases play pivotal roles in the processes of invasion, nutrition acquisition, immune evasion, and other host-parasite interactions. hundreds of genes encoding putative proteases have been identified in the recently published schistosome genomes. however, the expression profiles of these proteases in schistosoma species have not yet been systematically analyzed. we retrieved and culled the redundant protease sequences of schistosoma japonicum, schistosoma mansoni, echinococcus mul ... | 2014 | 25275570 |
| abnormal liver function in different patients with schistosoma japonicum. | schistosomiasis japonica, caused by schistosoma japonicum, is still a serious public health problem in china. it is important for schistosomiasis control to prevent from infection and advanced patients. recent years, however, the form of the prevalence of schistosomiasis japonica in china was changed these days. paying attention to the quality of life of these patients already infected with s. japonicum becomes a new objective to schistosomiasis control program. although most of the chronic infe ... | 2015 | 25287714 |
| immune regulation in schistosomiasis japonica. | in schistosomiasis caused by the blood fluke, schistosoma japonicum, the major lesion responsible for clinical disease is the granuloma formed in the liver as an immune response to the eggs deposited there. in this review abram stavitsky discusses the most recent evidence for the mechanisms involved in the formation and spontaneous regression of the granulomas and presents a working hypothesis to guide future research. | 1987 | 25290435 |
| cloning, expression and characterization of protein disulfide isomerase of schistosoma japonicum. | the excretory/secretory (es) proteins of schistosomes play important roles in modulating host immune systems and are regarded as potential vaccine candidates and drug targets. protein disulfide isomerase (pdi) is an essential enzyme that is involved in disulfide bond formation and rearrangement. in the present study, sjpdi, a 52.8 kda protein previously identified in a proteomics analysis as one of the es proteins of schistosoma japonicum, was cloned and characterized. western blot analysis show ... | 2014 | 25300760 |
| the affinity of magnetic microspheres for schistosoma eggs. | schistosomiasis is a chronic parasitic disease of humans, with two species primarily causing the intestinal infection: schistosoma mansoni and schistosoma japonicum. traditionally, diagnosis of schistosomiasis is achieved through direct visualisation of eggs in faeces using techniques that lack the sensitivity required to detect all infections, especially in areas of low endemicity. a recently developed method termed helmintex™ is a very sensitive technique for detection of schistosoma eggs and ... | 2015 | 25305086 |
| evaluating the effect of a novel molluscicide in the endemic schistosomiasis japonica area of china. | oncomelania hupensis is the sole intermediate host snail of schistosoma japonicum in china. snail control by molluscicide remains one of the most effective measures of schistosomiasis japonica control. a 50% wettable powder of niclosamide ethanolamine salt (wpn) is widely used for snail control in china. however, wpn is costly and toxic to fish. a novel molluscicide named lds, the salt of quinoid-2', 5-dichloro-4'-nitrosalicylanilide from niclosamide, has been developed. to evaluate the effects ... | 2014 | 25310539 |
| site-selective protein immobilization by covalent modification of gst fusion proteins. | the immobilization of functional proteins onto solid supports using affinity tags is an attractive approach in recent development of protein microarray technologies. among the commonly used fusion protein tags, glutathione s-transferase (gst) proteins have been indispensable tools for protein-protein interaction studies and have extensive applications in recombinant protein purification and reversible protein immobilization. here, by utilizing pyrimidine-based small-molecule probes with a sulfon ... | 2014 | 25340706 |
| [assessment of schistosomiasis endemic situation in national surveillance sites in hubei province from 2005 to 2010]. | to assess the schistosomiasis endemic situation in the national surveillance sites in hubei province, so as to provide the evidence for the prevention and control of schistosomiasis. | 2014 | 25345149 |
| [establishment of archives of schistosoma japonicum antibody indirect hemagglutination tests]. | to establish written and electronic archives of schistosoma japonicum antibody indirect hemagglutination (iha) tests. | 2014 | 25345170 |
| fluorometric titration assay of affinity of tight-binding nonfluorescent inhibitor of glutathione s-transferase. | to determine inhibition constant (k(i)) of tight-binding inhibitor, the putative method estimated an apparent k(i) from the response of initial rates to total concentrations of the inhibitor considering its depletion during binding for conversion into the true k(i), but was impractical with glutathione s-transferase of sophisticated kinetics. a fluorometric titration assay of dissociation constant (k(d)) was thus proposed. schistosoma japonicum glutathione s-transferase (sjgst) action on a nonfl ... | 2015 | 25349115 |
| vaccines and diagnostics for zoonotic schistosomiasis japonica. | schistosomiasis is one of the most prevalent, insidious and serious of the tropical parasitic diseases. although the effective anthelmintic drug, praziquantel, is widely available and cheap, it does not protect against re-infection, drug-resistant schistosome may evolve and mass drug administration programmes based around praziquantel are probably unsustainable long term. whereas protective anti-schistosome vaccines are not yet available, the zoonotic nature of schistosoma japonicum provides a n ... | 2015 | 25359506 |
| omega-1 knockdown in schistosoma mansoni eggs by lentivirus transduction reduces granuloma size in vivo. | schistosomiasis, one of the most important neglected tropical diseases worldwide, is caused by flatworms (blood flukes or schistosomes) that live in the bloodstream of humans. the hepatointestinal form of this debilitating disease results from a chronic infection with schistosoma mansoni or schistosoma japonicum. no vaccine is available to prevent schistosomiasis, and treatment relies predominantly on the use of a single drug, praziquantel. in spite of considerable research effort over the years ... | 2014 | 25400038 |
| research and control of advanced schistosomiasis japonica in china. | among the three main schistosomes (schistosoma japonicum, schistosoma mansoni, and schistosoma haematobium) known to infect humans, s. japonicum causes the most serious pathological lesions. in china, only schistosomiasis japonica is transmitted. from the 1950s, massive epidemiological investigations and active control measures for schistosomiasis japonica have been carried out. at the early stage of schistosomiasis control program, there were about 12 million schistosomiasis patients, and about ... | 2015 | 25403379 |
| atp synthase: an identified target gene of bantam in paired female schistosoma japonicum. | micrornas (mirnas) are a class of small non-coding rnas that function in transcriptional and post-transcriptional regulation of gene expression. an increasing number of schistosome mirnas have been identified and are expected possibly involved in differentiation, development, and metabolism. however, limited information is available concerning the target genes of schistosome mirnas. in the present study, the key target genes of bantam, an abundant mirna found in paired female schistosoma japonic ... | 2015 | 25407126 |
| [clinical manifestations and treatment of schistosomiasis hematobia]. | schistosomiasis hematobia is one major human schistosomiasis. the disease is endemic in africa and mediterra- nean region, and is the main cause of urogenital diseases. although only schistosoma japonicum is spreading across the mainland china, now more schistosomiasis hematobia cases are reported among aid projects and migrant workers to africa, with the economy development and the increasing degree of foreign exchanges. meanwhile, the relevant clinical data of.schistosomiasis hematobia are rar ... | 2014 | 25434130 |
| [cloning, expression and immuno-protection analysis of a gene encoding troponin t of schistosoma japonicum (sjtnt)]. | to clone cdna encoding troponin t of schistosoma japonicum (sjtnt), and evaluate the protective efficacy induced by recombinant sjtnt in balb/c mice against s. japonicum challenge infection. | 2014 | 25434137 |
| [influence of kaempferol on tgf-β1/smads signal path in liver tissue of mice with schistosoma japonicum infection]. | to investigate the influence of kaempferol on transforming growth factor(tgf-β1/smads signal tiransduction in liver tissue of mice with schistosomiasis liver fibrosis. | 2014 | 25434138 |
| [protective effect of fangyouling on schistosoma japonicum infection]. | to evaluate the protective effect of fangyouling (extracted from herb) on schistosomajaponicum infection in the field. | 2014 | 25434157 |
| hypoxia-inducible factor-1alpha regulates autophagy to activate hepatic stellate cells. | the role of autophagy in hif-1α modulated activation of hepatic stellate cells was illustrated in current work. autophagy markers were determined in livers of schistosoma japonicum infected mice and hypoxia or lps treated human hepatic stellate cell, lx-2 cells. the action of hif-1 to autophagy was defined as increase of autophagy markers was significantly suppressed in hif-1α sirna transfected cells upon hypoxia or lps stimulation. the function of autophagy in activation of lx-2 cells was asses ... | 2014 | 25450397 |
| pilot study on interferon-γ-producing t cell subsets after the protective vaccination with radiation-attenuated cercaria of schistosoma japonicum in the miniature pig model. | clawn miniature pig has been shown to serve as a suitable host for the experimental infection of schistosoma japonicum. in this study, we found that radiation-attenuated cercaria (rac) vaccine gave clawn miniature pigs protective immunity against subsequent challenge infection with s. japonicum cercaria. to characterize the protective immune response of the pig model vaccinated by attenuated cercaria, flow cytometric analysis of the reactive t cell subsets was performed. the intracellular interf ... | 2014 | 25473375 |
| il-22 and il-22 binding protein (il-22bp) regulate fibrosis and cirrhosis in hepatitis c virus and schistosome infections. | interleukin (il)-22 acts on epithelia, hepatocytes, and pancreatic cells and stimulates innate immunity, tissue protection, and repair. il-22 may also cause inflammation and abnormal cell proliferation. the binding of il-22 to its receptor is competed by il-22 binding protein (il-22bp), which may limit the deleterious effects of il-22. the role of il-22 and il-22bp in chronic liver diseases is unknown. we addressed this question in individuals chronically infected with schistosomes or hepatitis ... | 2015 | 25476703 |
| the anterior esophageal region of schistosoma japonicum is a secretory organ. | the esophagus of blood-feeding schistosomes has been largely neglected although its posterior portion was designated as a gland decades ago. however, we recently showed it plays a pivotal role in blood processing. it is clearly demarcated into anterior and posterior compartments, both surrounded by a mass of cell bodies. feeding movies revealed that erythrocytes accumulate in the anterior compartment before entering the posterior, indicating that a distinct process is executed there. we therefor ... | 2014 | 25490864 |
| [clinical manifestations and treatment of schistosomiasis hematobia]. | schistosomiasis hematobia is one major human schistosomiasis. the disease is endemic in africa and mediterra- nean region, and is the main cause of urogenital diseases. although only schistosoma japonicum is spreading across the mainland china, now more schistosomiasis hematobia cases are reported among aid projects and migrant workers to africa, with the economy development and the increasing degree of foreign exchanges. meanwhile, the relevant clinical data of.schistosomiasis hematobia are rar ... | 2014 | 25507728 |
| [cloning, expression and immuno-protection analysis of a gene encoding troponin t of schistosomajaponicum (sjtnt)]. | to clone cdna encoding troponin t of schistosoma japonicum (sjtnt), and evaluate the protective efficacy induced by recombinant sjtnt in balb/c mice against s. japonicum challenge infection. | 2014 | 25507735 |
| [influence of kaempferol on tgf-β1/smads signal path in liver tissue of mice with schistosoma japonicum infection]. | to investigate the influence of kaempferol on transforming growth factor(tgf-β1/smads signal tiransduction in liver tissue of mice with schistosomiasis liver fibrosis. | 2014 | 25507736 |
| the molecular characterization and rnai silencing of sjzfp1 in schistosoma japonicum. | during development, schistosoma japonicum undergoes many morphological and physiological transformations as a result of profound changes in gene expression. proteins containing zinc finger motifs usually play an important role in dna recognition, rna packaging, and transcriptional activation. in our current study, we cloned the open reading frame (orf) of sjzfp1 of s. japonicum, which encodes a zinc finger protein. we analyzed the complementary dna (cdna) sequence of sjzfp1 and examined the expr ... | 2015 | 25512211 |
| efficacy and safety of different dosages of praziquantel for the treatment of schistosoma japonicum: a systematic review and meta-analysis. | praziquantel, an antischistosomal compound, is used as first-line drug for chemotherapy of schistosoma japonicum since 1984. in this article, we conducted a systematic review and mete-analysis to evaluate the efficacy and safety of different dosages of praziquantel (pzq) for treatment of schistosoma japonicum. | 2014 | 25558390 |
| expression of septin4 in schistosoma japonicum-infected mouse livers after praziquantel treatment. | septin4 (sept4) exists widely in human tissues and is related to mechanical stability, actin dynamics, membrane trafficking, viral replication and apoptosis. data from many studies have suggested that sept4 plays a significant role in liver fibrosis. sept4 is down-regulated in the model of ccl4 and bdl treated liver fibrosis. however, it is up-regulated and peaked at 12 weeks post-infection (p.i.), and then decreased subsequently in schistosoma japonicum (s. japonicum) egg-induced liver fibrosis ... | 2015 | 25582427 |
| associations between schistosomiasis and the use of human waste as an agricultural fertilizer in china. | human waste is used as an agricultural fertilizer in china and elsewhere. because the eggs of many helminth species can survive in environmental media, reuse of untreated or partially treated human waste, commonly called night soil, may promote transmission of human helminthiases. | 2015 | 25590142 |
| elimination of schistosomiasis japonica from formerly endemic areas in mountainous regions of southern china using a praziquantel regimen. | schistosomiasis japonica is a major public health problem in china. domestic animals play a major role in the transmission of schistosoma japonicum to humans. to better understand the epidemiology of schistosomiasis japonica in domestic animals in the mountainous areas of china, we performed a 5-year longitudinal study of schistosomiasis in cattle and horses in yunnan province from 2009 to 2013. we also performed a concurrent drug-based intervention study in three settlement groups in yunnan pro ... | 2015 | 25591407 |
| development and optimization of cocktail-elisa for a unified surveillance of zoonotic schistosomiasis in multiple host species. | the zoonotic characteristic of the human parasite schistosoma japonicum infecting a significant number of wild and domestic animals highlights the need to develop a unified surveillance in multiple host species for a strengthened schistosomiasis control. it has been shown in several studies that water buffaloes and dogs are considered important reservoirs in the transmission of the schistosome parasite to humans. recombinant antigens like thioredoxin peroxidase-1 (sjtpx-1) and tandem repeat prot ... | 2015 | 25595656 |
| immunogenicity and anti-fecundity effect of nanoparticle coated glutathione s-transferase (sjgst) dna vaccine against murine schistosoma japonicum infection. | there is still urgent need for a vaccine against schistosomiasis, especially in schistosoma japonicum endemic areas where even a vaccine that will interrupt zoonotic transmission will be potentially effective as an intervention tool. we had developed a novel nanoparticle gene delivery system, which has proven efficacious in gene transfection to target immune cells with complementary adjuvant effect and high protective efficacy in several diseases. here, we applied this nanoparticle system in com ... | 2015 | 25603531 |
| an association of aquaporin-4 with the immunoregulation of liver pathology in mice infected with schistosoma japonicum. | schistosomiasis is a chronic parasitic disease that affects approximately 200 million people. in schistosomiasis japonica and mansoni, parasite eggs were trapped in host liver and stimulated the cd4(+)t cell responses to regulate the formation of the granulomas. subsequently, excessive granulomatous response in some heavily, and/or repeatedly infected individuals could result in chronic liver fibrosis and circulatory impairment. thus, elucidation of the mechanisms of these responses will not onl ... | 2015 | 25604731 |
| real-time pcr demonstrates high prevalence of schistosoma japonicum in the philippines: implications for surveillance and control. | the philippines has a population of approximately 103 million people, of which 6.7 million live in schistosomiasis-endemic areas with 1.8 million people being at risk of infection with schistosoma japonicum. although the country-wide prevalence of schistosomiasis japonica in the philippines is relatively low, the prevalence of schistosomiasis can be high, approaching 65% in some endemic areas. of the currently available microscopy-based diagnostic techniques for detecting schistosome infections ... | 2015 | 25606851 |
| transdermal praziquantel administration attenuates hepatic granulomatosis in mice infected with schistosoma japonicum. | liver granuloma is a major pathogenic factor responsible for schistosomiasis, and no effective drugs or therapy methods to treat it have been found so far. praziquantel (pzq) has shown some anti-schistosomal effect, but little information is available about the effect of pzq-prolonged administration on granuloma formation around schistosome eggs. herein, we investigated the effect of pzq on hepatic granuloma formation by treating the mice infected with schistosoma japonicum using a long-term pzq ... | 2015 | 25630695 |
| identification and characterization of endogenous viral elements for the three key schistosomes of humans. | endogenous viral elements (eves) are widely distributed throughout eukaryotic genomes, and their evolution and potential function have attracted a lot of interest. draft genome sequences for schistosoma mansoni, schistosoma japonicum and schistosoma haematobium are now available; however, information about eves in blood flukes of the genus schistosoma is scanty. here, genome-wide survey into the putative eve sequences of the three key schistosome genomes were present. totally 4, 117 gene sequenc ... | 2015 | 25631516 |
| high prevalence of schistosoma japonicum and fasciola gigantica in bovines from northern samar, the philippines. | the cause of zoonotic schistosomiasis in the philippines is schistosoma japonicum, which infects up to 46 mammalian hosts, including humans and bovines. in china, water buffaloes have been identified as major reservoir hosts for schistosomiasis japonica, contributing up to 75% of human transmission. in the philippines, water buffaloes (carabao; bubalus bubalis carabanesis) have, historically, been considered unimportant reservoirs. we therefore revisited the possible role of bovines in schistoso ... | 2015 | 25643317 |
| atypical presentation of cerebral schistosomiasis four years after exposure to schistosoma mansoni. | schistosomiasis is the second most socioeconomically devastating parasitic disease worldwide, affecting over 240 million people in 77 countries on 5 continents and killing 300,000 people annually in sub-saharan africa alone. neuroschistosomiasis is caused by granuloma formation around eggs that lodge in the cns, with schistosoma mansoni and schistosoma haematobium usually affecting the spinal cord and schistosoma japonicum causing most reported cerebral disease. we report a case of a previously ... | 2014 | 25667876 |
| vector-mediated microrna-21 silencing ameliorates granulomatous liver fibrosis in schistosoma japonicum infection. | 2015 | 25684616 | |
| human schistosomiasis: clinical perspective: review. | the clinical manifestations of schistosomiasis pass by acute, sub acute and chronic stages that mirror the immune response to infection. the later includes in succession innate, th1 and th2 adaptive stages, with an ultimate establishment of concomitant immunity. some patients may also develop late complications, or suffer the sequelae of co-infection with other parasites, bacteria or viruses. acute manifestations are species-independent; occur during the early stages of invasion and migration, w ... | 2013 | 25685450 |
| schistosomiasis in a migrating population in the lake region of china and its potential impact on control operation. | coverage of migrating people in schistosomiasis control program is a growing concern in china. schistosomiasis caused by schistosoma japonicum is still one of the major infectious diseases of public health importance in china though tremendous efforts have been made to control the transmission over the past decades. along with the rapid social-economic development, migrant population has been remarkably increasing across the country. the infected migrants may introduce a new souse of infection t ... | 2015 | 25700711 |
| screening diagnostic candidates for schistosomiasis from tegument proteins of adult schistosoma japonicum using an immunoproteomic approach. | schistosomiasis is one of the world's most prevalent zoonotic diseases and a serious worldwide public health problem. since the tegument (tg) of schistosoma japonicum is in direct contact with the host and induces a host immune response against infection, the identification of immune response target molecules in the schistosome tg is crucial for screening diagnostic antigens for this disease. | 2015 | 25706299 |
| [changes in splenocyte proliferation, subsets and cytokine production in mice immunized with recombinant vaccine bifidobacterium bifidum (pgex-sj32) of schistosoma japonicum]. | to observe the dynamic changes of immune responses of splenocytes in mice immunized with recombinant vaccine bifidobacterium bifidum (pgex-sj32) of schistosoma japonicum and investigate the immunological mechanism of the vaccine. | 2015 | 25736113 |
| perforation of small bowel caused by schistosoma japonicum: a case report. | a 67-year-old man from jingzhou was admitted to the first hospital affiliated to yangtze university in july 2013 with sudden onset of abdominal pain with dizziness for 12 h. the patient had sign of peritoneal irritation. ultrasonography of the abdomen and pelvis showed hepatic fibrosis due to schistosomiasis. computed tomography showed free gas in the peritoneal cavity. plain abdominal radiography showed bilateral subdiaphragmatic accumulation of gas, perforation of the viscus, and radio-opacity ... | 2015 | 25759563 |
| a novel schistosoma japonicum endonuclease homologous to dnase ii. | recent advances in studies of the schistosoma japonicum genome have opened new avenues for the elucidation of parasite biology and the identification of novel targets for vaccines, drug development and early diagnostic tools. | 2015 | 25766859 |
| identification and characterization of a member of rab subfamily, rab8, from clonorchis sinensis. | the rabs act as a binary molecular switch that utilizes the conformational changes associated with the gtp/gdp cycle to elicit responses from target proteins. it regulates a broad spectrum of cellular processes including cell proliferation, cytoskeletal assembly, and intracellular membrane trafficking in eukaryotes. the rab8 from clonorchis sinensis (csrab8) was composed of 199 amino acids. the deduced amino acid sequence shared above 50% identities with other species from trematode, tapeworm, m ... | 2015 | 25773178 |
| de novo transcriptome analysis of oncomelania hupensis after molluscicide treatment by next-generation sequencing: implications for biology and future snail interventions. | the freshwater snail oncomelania hupensis is the only intermediate host of schistosoma japonicum, which causes schistosomiasis. this disease is endemic in the far east, especially in mainland china. because niclosamide is the only molluscicide recommended by the world health organization, 50% wettable powder of niclosamide ethanolamine salt (wpn), the only chemical molluscicide available in china, has been widely used as the main snail control method for over two decades. recently, a novel mollu ... | 2015 | 25775015 |
| [surveillance and risk assessment system of schistosomiasis in jiangsu province. i. distribution of surveillance site and effectiveness of the system]. | to establish the surveillance and risk assessment system of schistosomiasis in jiangsu province, so as to provide technical support for timely understanding of the risk of schistosomiasis transmission and implementation of targeted control measures. | 2014 | 25782245 |
| [surveillance and forecast system of schistosomiasis in jiangsu province. vi. detection technology of water infectivity based on enrichment of schistosoma japonicum cercariae on water surface]. | to explore the enrichment technique of schistosoma japonicum cercariae on the water surface, so as to establish a new method combined with the existing technology to detect the cercarial infested water body quickly and sensitively. | 2014 | 25782246 |
| enhancement of protective efficacy through adenoviral vectored vaccine priming and protein boosting strategy encoding triosephosphate isomerase (sjtpi) against schistosoma japonicum in mice. | schistosomiasis japonica is a zoonotic parasitic disease; developing transmission blocking veterinary vaccines are urgently needed for the prevention and control of schistosomiasis in china. heterologous prime-boost strategy, a novel vaccination approach, is more effective in enhancing vaccine efficacy against multiple pathogens. in the present study, we established a novel heterologous prime-boost vaccination strategy, the radv-sjtpi.opt intramuscular priming and rsjtpi subcutaneous boosting st ... | 2015 | 25793406 |
| comparison of genetic diversity and population structure between two schistosoma japonicum isolates--the field and the laboratory. | schistosomiasis japonicum is one of the most important human parasitic diseases, and a number of studies have recently elucidated the difference in biological characteristics of s. japonicum among different parasite isolates, for example, between the field and the laboratory isolates. therefore, the understanding of underlying genetic mechanism is of both theoretical and practical importance. in this study, we used six microsatellite markers to assess genetic diversity, population structure, and ... | 2015 | 25804973 |
| expression, characterization and crystal structure of thioredoxin from schistosoma japonicum. | schistosoma japonicum, a human blood fluke, causes a parasitic disease affecting millions of people in asia. thioredoxin-glutathione system of s. japonicum plays a critical role in maintaining the redox balance in parasite, which is a potential target for development of novel antischistosomal agents. here we cloned the gene of s. japonicum thioredoxin (sjtrx), expressed and purified the recombinant sjtrx in escherichia coli. functional assay shows that sjtrx catalyses the dithiothreitol (dtt) re ... | 2015 | 25810021 |
| quantitative proteomic analyses of schistosoma japonicum in response to artesunate. | artesunate (art) has high prophylactic efficacy against schistosoma japonicum infections and has been used to treat and prevent schistosomiasis in china since 1995. however, the molecular mechanism of art's effects on s. japonicum remains unclear. herein, we applied isobaric tagging reagents for relative and absolute quantification analyses coupled with two-dimensional liquid chromatography and tandem mass spectrometry to investigate the effect of art on the proteome of s. japonicum in susceptib ... | 2015 | 25820832 |
| lack of il-17 signaling decreases liver fibrosis in murine schistosomiasis japonica. | accumulating evidence has identified the profibrogenic properties of il-17a in organ fibrosis. however, the role of il-17a signal in liver fibrosis induced by schistosoma japonicum infection remains unclear. in this study, we investigated liver fibrosis in wild-type (wt) and il-17ra(-/-) mice upon s. japonicum infection. hepatic il-17a, il-17c, il-17e (il-25), il-17f, il-17ra, il-17rb and il-17rc transcript levels were determined by rt-pcr. il-17a(+) cells were analyzed by flow cytometry and con ... | 2015 | 25840437 |
| spatial analysis of schistosomiasis in hubei province, china: a gis-based analysis of schistosomiasis from 2009 to 2013. | schistosomiasis remains a major public health problem in china. the major endemic areas are located in the lake and marshland regions of southern china, particularly in areas along the middle and low reach of the yangtze river. spatial analytical techniques are often used in epidemiology to identify spatial clusters in disease regions. this study assesses the spatial distribution of schistosomiasis and explores high-risk regions in hubei province, china to provide guidance on schistosomiasis con ... | 2015 | 25849567 |
| exosome-like vesicles derived by schistosoma japonicum adult worms mediates m1 type immune- activity of macrophage. | exosomes are 30-100-nm membrane vesicles of endocytic origin that are released into the extracellular space upon fusion of the multi-vesicular bodies (mvb) with the plasma membrane, while initial studies described that the role of exosomes was a reticulocyte cargo-disposal mechanism allowing remodeling of the plasma membrane during the maturation of reticulocytes to erythrocytes. recent studies indicate that exosomes are secreted by most cells and pathogens and play an important role in intercel ... | 2015 | 25855345 |
| [preliminary studies on effect of polysaccharides from tegillarca granosa as an adjuvant on dna vaccine against schistosoma japonicum infection]. | to discuss the effect of polysaccharides from tegillarca granosa as an adjuvant on pcdna3.1-sj26gst vaccine against schistosoma japonicum infection. | 2014 | 25856888 |
| [distribution characteristics of deposited eggs and pathological changes in viscera of new zealand white rabbits infected with schistosoma japonicum at different time]. | to study the distribution characteristics of deposited eggs and pathological changes in the viscera of animal infected with schistosoma japonicum at different time. | 2014 | 25856889 |
| multiplex real-time pcr monitoring of intestinal helminths in humans reveals widespread polyparasitism in northern samar, the philippines. | the global socioeconomic importance of helminth parasitic disease is underpinned by the considerable clinical impact on millions of people. while helminth polyparasitism is considered common in the philippines, little has been done to survey its extent in endemic communities. high morphological similarity of eggs between related species complicates conventional microscopic diagnostic methods which are known to lack sensitivity, particularly in low intensity infections. multiplex quantitative pcr ... | 2015 | 25858090 |
| exploring the contribution of host susceptibility to epidemiological patterns of schistosoma japonicum infection using an individual-based model. | we recently reported the analysis of epidemiological data suggesting variability in individual susceptibility to infection by schistosoma japonicum among rural villagers who reside in sichuan province of southwestern china. by supplementing the data used in the earlier analysis from other studies we have reported from this region, we presented improved estimates of cercarial exposure, which in turn, result in stronger evidence of susceptibility. this analysis was conducted using an individual-ba ... | 2015 | 25870427 |
| dna detection of schistosoma japonicum: diagnostic validity of a lamp assay for low-intensity infection and effects of chemotherapy in humans. | schistosomiasis has decreased significantly in prevalence and intensity of infection in china, thus more accurate and sensitive methods are desperately needed for the further control of schistosomiasis. the present work aimed to assess the utility of the loop-mediated isothermal amplification (lamp) for detection of light intensity infection or false-negative patients and patients post-treatment, targeting the highly repetitive retrotransposon sjr2 of schistosoma japonicum. | 2015 | 25874964 |
| geographical genetic structure of schistosoma japonicum revealed by analysis of mitochondrial dna and microsatellite markers. | schistosoma japonicum is a significant public health risk in parts of china and elsewhere in southeast asia. to gain an insight into the epidemiology of schistosomiasis japonica, a detailed investigation of s. japonicum genetic population structure is needed. | 2015 | 25881113 |
| shifts in the spatiotemporal dynamics of schistosomiasis: a case study in anhui province, china. | the chinese national surveillance system showed that the risk of schistosoma japonicum infection fluctuated temporally. this dynamical change might indicate periodicity of the disease, and its understanding could significantly improve targeted interventions to reduce the burden of schistosomiasis. the goal of this study was to investigate how the schistosomiasis risk varied temporally and spatially in recent years. | 2015 | 25881189 |
| altered levels of circulating mirnas are associated schistosoma japonicum infection in mice. | dioecious flatworms of the genus schistosoma causes schistosomiasis, which is a major public health problem in developing countries. acquiring detailed knowledge of schistosome-host interactions may aid in the development of novel strategies for schistosomiasis control. micrornas (mirnas) are involved in processes such as development, cell proliferation, metabolism, and signal transduction. circulating mirnas not only serve as a novel class of biomarkers of many diseases but also regulate target ... | 2015 | 25885182 |
| comparative characterization of micrornas in schistosoma japonicum schistosomula from wistar rats and balb/c mice. | more than 40 kinds of mammals in china are known to be naturally infected with schistosoma japonicum (s. japonicum) (peng et al. parasitol res 106:967-76, 2010). compared with permissive balb/c mice, rats are less susceptible to s. japonicum infection and are considered to provide an unsuitable microenvironment for parasite growth and development. micrornas (mirnas), via the regulation of gene expression at the transcriptional and post-transcriptional levels, may be responsible for developmental ... | 2015 | 25895062 |
| immunoproteomic analysis of schistosoma japonicum schistosomulum proteins recognized by immunoglobulin g in the sera of susceptible and non-susceptible hosts. | the aim of this study was to search for immunogenic schistosomula proteins in the hope of identifying novel intervention targets. schistosomula proteins were analyzed by immunoproteomic which the probes were sera derived from balb/c mice (susceptible hosts) and microtus fortis (resistant hosts). a total of 116 immunoreactive proteins recognized by 10 days post-infected balb/c mice, m. fortis sera, and uninfected m. fortis sera were selected for further analysis. finally, 95 protein spots were id ... | 2015 | 25896740 |
| preliminary characterization and expression of vasa-like gene in schistosoma japonicum. | the vasa gene is a vital germline marker to study the origin and development of germ cells and gonads in many organisms. until now, little information was available about the characteristics of the vasa gene in schistosoma japonicum (s. japonicum). in this study, we cloned the open reading frame (orf) of the s. japonicum vasa-like gene (sj-vasa). the expression pattern and tissue localization of sj-vasa were also analyzed. our results showed that sj-vasa shared the general feature of dead-box fa ... | 2015 | 25899325 |
| a specific indel marker for the philippines schistosoma japonicum revealed by analysis of mitochondrial genome sequences. | in the present study, near-complete mitochondrial (mt) genome sequences for schistosoma japonicum from different regions in the philippines and japan were amplified and sequenced. comparisons among s. japonicum from the philippines, japan, and china revealed a geographically based length difference in mt genomes, but the mt genomic organization and gene arrangement were the same. sequence differences among samples from the philippines and all samples from the three endemic areas were 0.57-2.12 a ... | 2015 | 25899327 |
| [analysis on parasitic infection of clinical samples from hospitals in shanghai during 2011-2013]. | to analyze the results of parasitic pathogen detection on clinical samples from shanghai hospitals during 2011-2013. | 2014 | 25902676 |
| protective immunity against schistosoma japonicum infection can be provided by igg antibodies towards periodate-sensitive or periodate-resistant glycans. | it has been well accepted that glycans present in schistosomes are highly antigenic. however, it is not clear what kind of worm glycans can affect the infected host to mount igg responses and whether mounted anti-glycan igg responses are protective. | 2015 | 25907161 |
| [reinfection of schistosoma japonicum and related risk factors in chinese: meta-analysis]. | to study the dynamics of the reinfection of schistosoma japonicum and related risk factors among the people in schistosomiasis endemic areas in china. | 2015 | 25907732 |
| hybrid steered molecular dynamics approach to computing absolute binding free energy of ligand-protein complexes: a brute force approach that is fast and accurate. | computing the free energy of binding a ligand to a protein is a difficult task of essential importance for which purpose various theoretical/computational approaches have been pursued. in this paper, we develop a hybrid steered molecular dynamics (hsmd) method capable of resolving one ligand–protein complex within a few wall-clock days with high enough accuracy to compare with the experimental data. this hsmd approach is based on the relationship between the binding affinity and the potential of ... | 2015 | 25937822 |
| interleukin-33 promotes th2 immune responses in infected mice with schistosoma japonicum. | il-33, a new member of the il-1 cytokine family, is associated with many infectious diseases. il-33 not only is crucial for induction of th2 polarized responses, but also is involved in induction of inflammation as a proinflammatory cytokine. whether il-33 leads to beneficial or worsening outcomes depends on the immune mechanism underlying the pathogensis of each disease condition. this study was to elucidate the role of il-33 in schistosomiasis japonica in a mouse model. our results demonstrate ... | 2015 | 25944738 |
| suppression of the insulin receptors in adult schistosoma japonicum impacts on parasite growth and development: further evidence of vaccine potential. | to further investigate the importance of insulin signaling in the growth, development, sexual maturation and egg production of adult schistosomes, we have focused attention on the insulin receptors (sjirs) of schistosoma japonicum, which we have previously cloned and partially characterised. we now show, by biolayer interferometry, that human insulin can bind the l1 subdomain (insulin binding domain) of recombinant (r)sjir1 and rsjir2 (designated sjld1 and sjld2) produced using the drosophila s2 ... | 2015 | 25961574 |
| an ex vivo model for studying hepatic schistosomiasis and the effect of released protein from dying eggs. | we report the use of an ex vivo precision cut liver slice (pcls) mouse model for studying hepatic schistosomiasis. in this system, liver tissue is unfixed, unfrozen, and alive for maintenance in culture and subsequent molecular analysis. | 2015 | 25965781 |
| m13 phage peptide zl4 exerts its targeted binding effect on schistosoma japonicum via alkaline phosphatase. | the present study was to determine the targeting effect of m13 phage peptide zl4 (mppzl4) on schistosoma japonicum (s.j). mice infected with s.j were injected with mppzl4. real-time pcr was used to detect the distribution and metabolism of mppzl4 in the livers and lungs of mice. in vivo refusion test was performed to detect the targeting of mppzl4. western blotting was employed to determine the expression of mppzl4. live imaging was used to detect the distribution of oligopeptide mppzl4. immunoh ... | 2015 | 25973009 |
| intake of erythrocytes required for reproductive development of female schistosoma japonicum. | the reproductive development and maturation of female schistosomes are crucial since their released eggs are responsible for the host immunopathology and transmission of schistosomiasis. however, little is known about the nutrients required by female schistosoma japonicum during its sexual maturation. we evaluated the promoting effect of several nutrients (calf serum, red blood cells (rbcs), atp and hypoxanthine) on the reproductive development of pre-adult females at 18 days post infection (dpi ... | 2015 | 25978643 |
| [cloning, expression and protective efficacy evaluation of radiation sensitive protein 23 (rad23) from schistosoma japonicum]. | radiation sensitive protein 23 (rad23) is a nucleotide excision repair (ner) protein that plays an important role in ubiquitin-proteasome pathway (upp). schistosoma japonicum radiation sensitive protein23 (sjrad23) cdna sequences were amplified by pcr and cloned into pet28a (+) vector to construct recombinant expression plasmid pet28a(+)-sjrad23. the recombinant protein was expressed as both inclusion bodies and the supernatant in escherichia coli bl21 (de3) cell. immunofluorescence observation ... | 2014 | 25985518 |
| tim-3 induces th2-biased immunity and alternative macrophage activation during schistosoma japonicum infection. | t cell immunoglobulin- and mucin-domain-containing molecule 3 (tim-3) has been regarded as an important regulatory factor in both adaptive and innate immunity. recently, tim-3 was reported to be involved in th2-biased immune responses in mice infected with schistosoma japonicum, but the exact mechanism behind the involvement of tim-3 remains unknown. the present study aims to understand the role of tim-3 in the immune response against s. japonicum infection. tim-3 expression was determined by fl ... | 2015 | 25987707 |
| design, synthesis and biological evaluation of praziquantel and endoperoxide conjugates as antischistosomal agents. | the widespread use of praziquantel for the treatment of schistosomiasis has led to concerns over the potential development of drug resistance. therefore, the discovery of novel antischistosomal agents is imperative. in this study, a series of praziquantel and endoperoxide conjugates were synthesized and evaluated as potential antischistosomal agents. | 2015 | 25996065 |
| sje16.7 activates macrophages and promotes schistosoma japonicum egg-induced granuloma development. | sje16.7 is an egg-specific protein from schistosoma japonicum that recruits neutrophils and initiates an inflammatory granuloma response in host tissue. however, since macrophages are known to be important regulators of egg granuloma formation we investigated the effect of sje16.7 on this cell type. here we report that sje16.7 is a potent macrophage activator, inducing macrophage chemotaxis and stimulating cytokine production. treatment of murine primary macrophages with sje16.7 resulted in upre ... | 2015 | 25997882 |
| the expression of molecule cd28 and cd38 on cd4⁺/cd8⁺ t lymphocytes in thymus and spleen elicited by schistosoma japonicum infection in mice model. | schistosomiasis caused by human schistosomes such as schistosoma japonicum (s. japonicum) is considered as an immune-related disease. it was demonstrated that specific cytokine antibodies' response elicited by s. japonicum infection was gradually downregulated with the progress of the disease, resulting in a th1/th2 polarization and suppression of immune response. cd28 (cluster of differentiation 28) is one of the proteins expressed on t cells that provide co-stimulatory signals required for t c ... | 2015 | 26002824 |
| cx3cr1 deficiency in mice attenuates hepatic granuloma formation during acute schistosomiasis by enhancing the m2-type polarization of macrophages. | acute schistosomiasis is characterized by pro-inflammatory responses against tissue- or organ-trapped parasite eggs along with granuloma formation. here, we describe studies in cx3cr1(-/-) mice and demonstrate the role of cx3cr1 in the pathoetiology of granuloma formation during acute schistosomiasis. mice deficient in cx3cr1 were protected from granuloma formation and hepatic injury induced by schistosoma japonicum eggs, as manifested by reduced body weight loss and attenuated hepatomegaly alon ... | 2015 | 26035381 |
| serological prevalence of schistosoma japonicum in mobile populations in previously endemic but now non-endemic regions of china: a systematic review and meta-analysis. | schistosomiasis japonica has been resurging in certain areas of china where its transmission was previously well controlled or interrupted. several factors may be contributing to this, including mobile populations, which if infected, may spread the disease. a wide range of estimates have been published for s. japonicum infections in mobile populations, and a synthesis of these data will elucidate the relative risk presented from these groups. | 2015 | 26043190 |
| specific humoral response of hosts with variable schistosomiasis susceptibility. | the schistosome blood flukes are some of the largest global causes of parasitic morbidity. further study of the specific antibody response during schistosomiasis may yield the vaccines and diagnostics needed to combat this disease. therefore, for the purposes of antigen discovery, sera and antibody-secreting cell (asc) probes from semi-permissive rats and sera from susceptible mice were used to screen a schistosome protein microarray. following schistosoma japonicum infection, rats had reduced p ... | 2016 | 26044065 |
| prevention of fatal hepatic complication in schistosomiasis by inhibition of cetp. | schistosoma japonicum, once endemic all the east asia, remains as a serious public health problem in certain regions. ectopic egg embryonation in the liver causes granulomatosis and eventually fatal cirrhosis, so that prevention of this process is one of the keys to reduce its mortality. the embryonation requires cholesteryl ester from hdl of the host blood for egg yolk formation, and this reaction is impaired from the abnormal large hdl in genetic cholesteryl ester transfer protein (cetp) defic ... | 2015 | 26060442 |
| the up-regulation of ribosomal proteins further regulates protein expression profile in female schistosoma japonicum after pairing. | pairing of schistosoma males and females leads to and maintains female sexual maturation. however, the mechanism by which pairing facilitates sexual maturation of females is not clear. an increasing body of evidence suggests that ribosomal proteins have regulatory rather than constitutive roles in protein translation. | 2015 | 26070205 |
| characterization and expression analysis of wnt5 in schistosoma japonicum at different developmental stages. | wnt signaling is a key pathway involving the regulation of cell development and growth in metazoa. an analysis of wnt signaling in schistosoma japonicum might provide information regarding the molecular mechanisms underlying parasite development, which might be useful for vaccine screening and identification of pharmaceutical targets. the sjwnt5 gene, a member of the wnt gene family, contained an 1149-bp open reading frame that encoded a 382-aa protein. analysis of the sjwnt5 amino acid sequence ... | 2015 | 26077755 |