Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
detection and differentiation of vibrio spp. in seafood and fish samples with cultural and molecular methods.vibrio spp. as natural inhabitants of sea- and brackwater of both tropical and temperate regions of the world are commonly found in different kinds of seafood. even among the three main human pathogenic species vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus most of the isolates from seafood do not carry the different virulence factors responsible for foodborne infections. therefore, the risk assessment of vibrio spp. in seafood is currently based mainly on the knowledge of the ge ...201020688407
[a case of vibrio vulnificus infection presenting with invagination].a 73-year-old man with advanced lung cancer ate raw fish the day before being admitted to our hospital with severe abdominal pain, fever and vomiting. soon afterward he fell into shock and disseminated intravenous coagulation. a ct scan revealed invagination and emergency surgery was performed. on the first day after surgery, vibrio vulnificus was cultured from his ascites and blood. the infection was controlled by antibiotic treatment and he was discharged 23 days after the surgery. vibrio vuln ...201020715528
dietary medicinal plant extracts improve growth, immune activity and survival of tilapia oreochromis mossambicus.the effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (bermuda grass cynodon dactylon, h(1), beal aegle marmelos, h(2), winter cherry withania somnifera, h(3) and ginger zingiber officinale, h(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia oreochromis mossambicus were assessed. in addition, the antimicrobial properties of the extract were assessed against vibrio alginolyticus, vibrioparahaemolyticus, vibri ...200920735646
occurrence of vibrio vulnificus in mussel farms from the varano lagoon environment.monitoring the occurrence of the human pathogen vibrio vulnificus in a mussel farm located in the lagoon of varano (italy).201020807335
current status and future prospects in a pathogenic study of vibrio vulnificus. 201020808058
role of gaca in virulence of vibrio vulnificus.the gacs/gaca two-component signal transduction system regulates virulence, biofilm formation and symbiosis in vibrio species. the present study investigated this regulatory pathway in vibrio vulnificus, a human pathogen that causes life-threatening disease associated with the consumption of raw oysters and wound infections. small non-coding rnas (csrb1, csrb2, csrb3 and csrc) commonly regulated by the gacs/gaca pathway were decreased (p<0.0003) in a v. vulnificus cmcp6 δgaca : : aph mutant comp ...201020817642
relationships between environmental factors and pathogenic vibrios in the northern gulf of mexico.although autochthonous vibrio densities are known to be influenced by water temperature and salinity, little is understood about other environmental factors associated with their abundance and distribution. densities of culturable vibrio vulnificus containing vvh (v. vulnificus hemolysin gene) and v. parahaemolyticus containing tlh (thermolabile hemolysin gene, ubiquitous in v. parahaemolyticus), tdh (thermostable direct hemolysin gene, v. parahaemolyticus pathogenicity factor), and trh (tdh-rel ...201020817802
booster biocides and microfouling.antifouling (af) paints are used to prevent the attachment of living organisms to the submerged surfaces of ships, boats and aquatic structures, usually by the release of biocides. apart from copper, organic booster biocides are the main active components in af paints, but their use can have a negative impact on the marine environment. the direct effects of biocides on marine bacteria are poorly known. this work investigates the impact of two biocides, viz. diuron and tolylfluanid, on the growth ...201020824571
ibuprofen augments pro-inflammatory cytokine release in a mouse model of vibrio vulnificus infection.we evaluated the effects of ibuprofen on cytokine production and mortality in a mouse model of septic shock induced by vibrio vulnificus, strain chi mei vv05191. ibuprofen (50 mg/kg) or saline (control) was given to female balb/cbyj mice for three consecutive days before exposure to the pathogen. for cytokine production, serum and peritoneal fluid were assayed for il-1β, il-6, tnf-α, and mip-2 by elisa at 3, 6, and 9 hr after intraperitoneal infection of the organism. at 6 hr after infection, se ...201020840153
solid sequencing of four vibrio vulnificus genomes enables comparative genomic analysis and identification of candidate clade-specific virulence genes.vibrio vulnificus is the leading cause of reported death from consumption of seafood in the united states. despite several decades of research on molecular pathogenesis, much remains to be learned about the mechanisms of virulence of this opportunistic bacterial pathogen. the two complete and annotated genomic dna sequences of v. vulnificus belong to strains of clade 2, which is the predominant clade among clinical strains. clade 2 strains generally possess higher virulence potential in animal m ...201020863407
co-culture supernatants from vibrio vulnificus-infected int-407 cells induce il-8 production in intestinal epithelial cells: crucial role of v. vulnificus rtxe.in a previous study, we reported that a gene mutation of repeat in toxin e (rtxe), a transporter of cytotoxic factors, resulted in a significant impairment of epithelial cell cytotoxicity in vibrio vulnificus, and that the expression of the rtxe gene was induced by the exposure to the host cells. in this study, we evaluated and compared the effects of co-culture supernatants from v. vulnificus-infected int-407 cells and either the v. vulnificus wild-type or rtxe mutant on the production of inter ...201020878086
a novel and simple pcr walking method for rapid acquisition of long dna sequence flanking a known site in microbial genome.acquisition of flanking sequence adjacent to a known dna site is an important task in microbial genome-related research. in this study, we developed a new method containing two rounds of pcr followed by cloning and sequencing. firstly, specific primer (sp) is added into the reaction system for primary locus-specific linear amplification, and then a complex long primer (clp) is added into the cooled reaction system for only one cycle. amplification products from the first round of pcr are directl ...201120878504
virulence characteristics of sucrose-fermenting vibrio vulnificus strains.we identified 6 sucrose-fermenting vibrio vulnificus strains and examined their virulence characteristics. they were all encapsulated, motile, capable of producing toxins and utilizing transferrin-bound iron, cytotoxic to cultured cells, and virulent enough to kill mice. they could be definitely identified only by genetic identification methods such as pcr, and not by conventional culture-based identification methods such as api 20e (biomérieux, france). these results indicate that it is essenti ...201020890083
evidence for the horizontal transfer of an unusual capsular polysaccharide biosynthesis locus in marine bacteria.the most intensely studied of the vibrio vulnificus virulence factors is the capsular polysaccharide (cps). all virulent strains produce copious amounts of cps. acapsular strains are avirulent. the structure of the cps from the clinical isolate atcc 27562 is unusual. it is serine modified and contains, surprisingly, n-acetylmuramic acid. we identified the complete 25-kb cps biosynthesis locus from atcc 27562. it contained 21 open reading frames and was allelic to o-antigen biosynthesis loci. two ...201020921143
macrophage migration inhibitory factor regulates interleukin-6 production by facilitating nuclear factor-kappa b activation during vibrio vulnificus infection.patients infected with vibrio vulnificus (v. vulnificus) show severe inflammatory responses characterised by the upregulation of proinflammatory cytokines. macrophage migration inhibitory factor (mif), an upstream proinflammatory regulator, increases the inflammation caused by sepsis. whether mif regulates responses to v. vulnificus infection and the actual mechanism by which v. vulnificus initiates these mif-modulated proinflammatory cytokines remain unclear.201020939898
[correlations between climate change-related infectious diseases and meteorological factors in korea].infectious diseases are known to be affected by climate change. we investigated if the infectious diseases were related to meteorological factors in korea.201020959714
a mammalian insulysin homolog is regulated by enzyme iia(glc) of the glucose transport system in vibrio vulnificus.vibrio vulnificus is an opportunistic human pathogen that causes severe infections in susceptible individuals. while the components of the escherichia coli phosphoenolpyruvate: sugar phosphotransferase system (pts) have been shown to regulate numerous targets, little such information is available for the v. vulnificus pts. here we show that enzyme iia(glc) of the pts regulates the peptidase activity of a mammalian insulysin homolog in v. vulnificus. while interaction of iia(glc) with the insulys ...201020971110
interactive effects of cadmium and hypoxia on metabolic responses and bacterial loads of eastern oysters crassostrea virginica gmelin.pollution by toxic metals including cadmium (cd) and hypoxia are important stressors in estuaries and coastal waters which may interactively affect sessile benthic organisms, such as oysters. we studied metabolic responses to prolonged hypoxic acclimation (2 weeks at 5% o2) in control and cd-exposed (30 d at 50 μg l(-1) cd) oysters crassostrea virginica, and analyzed the effects of these stressors on abundance of vibrio spp. in oysters. hypoxia-acclimated oysters retained normal standard metabol ...201020971492
sepsis and fulminant multi-organ failure associated with vibrio vulnificus infection. 201021038545
genetic analysis of the capsule polysaccharide (k antigen) and exopolysaccharide genes in pandemic vibrio parahaemolyticus o3:k6.pandemic vibrio parahaemolyticus has undergone rapid changes in both k- and o-antigens, making detection of outbreaks more difficult. in order to understand these rapid changes, the genetic regions encoding these antigens must be examined. in vibrio cholerae and vibrio vulnificus, both o-antigen and capsular polysaccharides are encoded in a single region on the large chromosome; a similar arrangement in pandemic v. parahaemolyticus would help explain the rapid serotype changes. however, previous ...201021044320
studies on a vibrio vulnificus functional ortholog of escherichia coli rnase e imply a conserved function of rnase e-like enzymes in bacteria.rnase e (rne) plays a key role in the processing and degradation of rna in escherichia coli. in the genome of vibrio vulnificus, one open reading frame potentially encodes a protein homologous to e. coli rnase e, designated rnase ev, which n-terminal (1-500 amino acids) has 86.4% amino acid identity to the n-terminal catalytic part of rnase e (n-rne). here, we report that both the full-length and the n-terminal part of rnase ev (n-rnev) functionally complement e. coli rnase e and their expressio ...201021046401
calcium promotes exopolysaccharide phase variation and biofilm formation of the resulting phase variants in the human pathogen vibrio vulnificus.vibrio vulnificus is a gram-negative bacterium found in estuaries and coastal waters and is associated with human disease caused by ingestion of raw shellfish. pathogenesis is directly related to the presence of capsular polysaccharide (cps). encapsulated virulent strains exhibit an opaque colony phenotype, while unencapsulated attenuated strains appear translucent. a third colony type, rugose, is caused by expression of rugose extracellular polysaccharide (reps) and forms robust biofilms. vibri ...201021059165
vibrio vulnificus dna load and mortality.we determined the association between dna load and mortality in patients with vibrio vulnificus infection. real-time pcr performed on sera of 27 culture-positive patients showed a significantly higher median dna load in nonsurvivors than in survivors. hence, real-time pcr can be used as an early prognostic factor in v. vulnificus septicemia.201021068289
conventional and molecular methods to detect bacterial pathogens in mussels.to detect aeromonas spp., salmonella spp., vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus in mussels and water samples from a farming area, conventional and molecular methods were applied to enrichment cultures.201021070267
domain organization and evolution of multifunctional autoprocessing repeats-in-toxin (martx) toxin in vibrio vulnificus.the objective of this study was to analyze multifunctional autoprocessing repeats-in-toxin (martx) toxin domain organization within the aquatic species vibrio vulnificus as well as to study the evolution of the rtxa1 gene. the species is subdivided into three biotypes that differ in host range and geographical distribution. we have found three different types (i, ii, and iii) of v. vulnificus martx (martx(vv)) toxins with common domains (an autocatalytic cysteine protease domain [cpd], an α/β-hy ...201021075892
a human single-chain variable fragment targeting to vibrio vulnificus rtxa toxin.vibrio vulnificus secretes a multifunctional cytotoxin rtxa (vvrtxa), which plays a major role in the bacterial pathogenesis. the lack of an efficient vvrtxa detection tool has hampered the progress of v. vulnificus pathogenesis research. this study aims to isolate vvrtxa specific single-chain variable fragments (scfv) to serve as a detection agent. the vvrtxa c-terminal gly-asp (gd) repeat-containing region, which has been implicated for calcium binding and target cell recognition, was chosen a ...201021081141
polyphyletic origin of vibrio vulnificus biotype 2 as revealed by sequence-based analysis.a sequence-based analysis of seven housekeeping and virulence-related genes shows that the species vibrio vulnificus is subdivided into three phylogenetic lineages that do not correspond with the biotypes and that biotype 2 is polyphyletic. these results support the reclassification of biotype 2 as a pathovar that would group the strains with pathogenic potential to develop vibriosis in fish.201021097581
cloning, characterization, and expression analysis of orange-spotted grouper (epinephelus coioides) ilf2 gene (ecilf2).interleukin-2 enhancer binding factor 2 (ilf2), also named as nuclear factor 45 (nf45), plays important roles in regulating interleukin-2 expression in mammals. in the present study, a novel ilf2 gene (designated ecilf2) was cloned and well characterized from orange-spotted grouper, epinephelus coioides. the full-length ecilf2 cdna is composed of 1544 bp and encodes a polypeptide of 387 amino acids with 98% identity to ilf2 of atlantic salmon. the genomic dna of ecilf2 consists of 14 exons and 1 ...201021109006
gram negative shuttle bac vector for heterologous expression of metagenomic libraries.bacterial artificial chromosome (bac) vectors enable stable cloning of large dna fragments from single genomes or microbial assemblages. a novel shuttle bac vector was constructed that permits replication of bac clones in diverse gram-negative species. the "gram-negative shuttle bac" vector (pgns-bac) uses the f replicon for stable single-copy replication in e. coli and the broad-host-range rk2 mini-replicon for high-copy replication in diverse gram-negative bacteria. as with other bac vectors c ...201021112378
the use of genetic typing methods to discriminate among strains of vibrio cholerae, v. parahaemolyticus, and v. vulnificus.this review article summarizes the findings of recent typing studies conducted on vibrio cholerae, v. parahaemolyticus, and v. vulnificus. the dna-based methods used to type the vibrio spp. include whole genome approaches, such as pulsed field gel electrophoresis (pfge), ribotyping, and repetitive extragenic palindromic (rep)-pcr, single gene targets, and multiple gene targets (multilocus approaches). the goals of these studies include establishing the relatedness of isolates from disease epidem ...201021140669
multiple antibacterial histone h2b proteins are expressed in tissues of american oyster.we have previously identified a histone h2b isomer (cvh2b-1) from tissue extracts of the bivalve mollusk, the american oyster (crassostrea virginica). in this paper, we isolate an additional three antibacterial proteins from acidified gill extract by preparative acid-urea-polyacrylamide gel electrophoresis and reversed-phase high performance liquid chromatography. extraction of these proteins from tissue was best accomplished by briefly boiling the tissues in a weak acetic acid solution. additio ...201021145411
molecular cloning and characterization of two novel hepcidins from orange-spotted grouper, epinephelus coioides.orange-spotted grouper, epinephelus coioides is one of the most important economic species of marine-cultured fish in china and southeast asia countries. however, very little information of the innate immune mechanisms against microbial pathogens is available in grouper, epinephelus sp. hepcidin, as an antimicrobial peptide (amp), is a very important component in the innate immune system and widespread in fish. in this study, two novel types of hepcidin gene (designated ec-hepcidin1 and ec-hepci ...201021145974
development of a single base extension-tag microarray for the detection of pathogenic vibrio species in seafood.in this study, a single base extension-tag array on glass slides (sbe-tags) microarray was established to detect the seven leading seafood-borne pathogens, including vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio mimicus, vibrio alginolyticus, vibrio anguillarum, and vibrio harveyi. three multiplex pcr assays were developed to specifically target the following species with individual gene markers, which are aads, tdh, and trh for v. parahaemolyticus; col, toxr, and vvh for v ...201021161212
putative virulence factors identified in vibrio vulnificus strains isolated from oysters and seawater in mexico.the presence of vibrio vulnificus was analyzed in oyster and estuarine water samples from mexico by pcr amplification of the vvha gene and some putative virulence factors were tested. samples were collected from 12 different sampling points over a one-year period; 31% samples were positive for v. vulnificus and all isolates were identified as biotype 1. all strains were cytotoxic and proteolytic, 98% showed adherence to epithelial cells, 91.4% were dnase-positive, 77.6% were mucinase-positive, 9 ...201021161801
a comprehensive review of vibrio vulnificus: an important cause of severe sepsis and skin and soft-tissue infection.vibrio vulnificus is a halophilic gram-negative bacillus found worldwide in warm coastal waters. the pathogen has the ability to cause primary sepsis in certain high-risk populations, including patients with chronic liver disease, immunodeficiency, iron storage disorders, end-stage renal disease, and diabetes mellitus. most reported cases of primary sepsis in the usa are associated with the ingestion of raw or undercooked oysters harvested from the gulf coast. the mortality rate for patients wit ...201021177133
fatal vibrio vulnificus infection associated with eating raw oysters, new caledonia. 201121192878
genotype is correlated with but does not predict virulence of vibrio vulnificus biotype 1 in subcutaneously inoculated, iron dextran-treated mice.vibrio vulnificus is the leading cause of reported deaths from infections related to consumption of seafood in the united states. affected predisposed individuals frequently die rapidly from sepsis. otherwise healthy people can experience severe wound infection, which can lead to sepsis and death. a question is why, with so many people consuming contaminated raw oysters, the incidence of severe v. vulnificus disease is low. molecular typing systems have shown associations of v. vulnificus genoty ...201121199909
bacterial diseases of crabs: a review.bacterial diseases of crabs are manifested as bacteremias caused by organisms such as vibrio, aeromonas, and a rhodobacteriales-like organism or tissue and organ tropic organisms such as chitinoclastic bacteria, rickettsia intracellular organisms, chlamydia-like organism, and spiroplasma. this paper provides general information about bacterial diseases of both marine and freshwater crabs. some bacteria pathogens such as vibrio cholerae and vibrio vulnificus occur commonly in blue crab haemolymph ...201121215353
vibrio vulnificus rtxa1 gene recombination generates toxin variants with altered potency during intestinal infection.vibrio vulnificus is a food-borne bacterial pathogen associated with 1% of all food-related deaths, predominantly because of consumption of contaminated seafood. the ability of v. vulnificus to cause disease is linked to the production of a large cytotoxin called the "multifunctional-autoprocessing rtx" (martx(vv)) toxin, a factor shown here to be an important virulence factor by the intragastric route of infection in mice. in this study, we examined genetic variation of the rtxa1 gene that enco ...201121220343
integrative genome-scale metabolic analysis of vibrio vulnificus for drug targeting and discovery.although the genomes of many microbial pathogens have been studied to help identify effective drug targets and novel drugs, such efforts have not yet reached full fruition. in this study, we report a systems biological approach that efficiently utilizes genomic information for drug targeting and discovery, and apply this approach to the opportunistic pathogen vibrio vulnificus cmcp6. first, we partially re-sequenced and fully re-annotated the v. vulnificus cmcp6 genome, and accordingly reconstru ...201121245845
evaluation of global gene expression during cold shock in the human pathogen vibrio vulnificus.vibrio vulnificus can adapt to cold temperatures by changing the expression profiles of certain genes and their resulting proteins. in this study, the complete v. vulnificus transcriptome was analyzed under cold shock by looking at gene expression changes occurring during the shift from 35°c to 4°c. a dna microarray-based global transcript profiling of v. vulnificus showed that 165 genes out of 4,488 altered their expression profiles by more than twofold. from 35°c to 10°c, an overall gene repre ...201121246233
structure-activity relationship of cinnamaldehyde analogs as inhibitors of ai-2 based quorum sensing and their effect on virulence of vibrio spp.many bacteria, including vibrio spp., regulate virulence gene expression in a cell-density dependent way through a communication process termed quorum sensing (qs). hence, interfering with qs could be a valuable novel antipathogenic strategy. cinnamaldehyde has previously been shown to inhibit qs-regulated virulence by decreasing the dna-binding ability of the qs response regulator luxr. however, little is known about the structure-activity relationship of cinnamaldehyde analogs.201121249192
[zoonotic bacterium: duty to notify advisable].vibrio vulnificus is a zoonotic bacterium that can be found in raw fish (mainly eel and oysters) and seawater. human infections may extend from wound infections to fasciitis necroticans or primary sepsis with a mortality rate of more than 50%. although v. vulnificus is mainly found in the usa, its worldwide spread is also likely to involve the netherlands, as demonstrated by an increasing number of infected fish farms. since 2007, v. vulnificus infections have become a notifiable infectious dise ...201121262025
comparison of necrotizing fasciitis and sepsis caused by vibrio vulnificus and staphylococcus aureus.vibrio vulnificus can cause a rapidly progressive fatal soft-tissue infection. staphylococcus aureus is the most common cause of skin and soft-tissue infections reported worldwide, and, in particular, methicillin-resistant staphylococcus aureus has emerged as the most common isolate in emergency departments. the purposes of the present study were to compare the specific characteristics of vibrio vulnificus and staphylococcus aureus infections and to compare the clinical outcomes of vibrio vulnif ...201121266641
comparative study between transcriptionally- and translationally-acting adenine riboswitches reveals key differences in riboswitch regulatory mechanisms.many bacterial mrnas are regulated at the transcriptional or translational level by ligand-binding elements called riboswitches. although they both bind adenine, the adenine riboswitches of bacillus subtilis and vibrio vulnificus differ by controlling transcription and translation, respectively. here, we demonstrate that, beyond the obvious difference in transcriptional and translational modulation, both adenine riboswitches exhibit different ligand binding properties and appear to operate under ...201121283784
crystal structure of toll-like receptor 2-activating lipoprotein iipa from vibrio vulnificus. 201021287630
identification and functional characterization of an interferon regulatory factor 7-like (irf7-like) gene from orange-spotted grouper, epinephelus coioides.interferon regulatory factor (irf) 7 plays a crucial role in modulating cellular responses to viral infection and cytokines, including interferons (ifns). in the present study, a novel irf7 gene (designated as ecirf7) was cloned and characterized from orange-spotted grouper, epinephelus coioides. the full-length ecirf7 cdna is composed of 2089 bp and encodes a polypeptide of 433 amino acids with 81% identity to irf7 of siniperca chuatsi, and the genomic dna of ecirf7 consists of 9 exons and 8 in ...201121295068
antibacterial activity of thai medicinal plants pikutbenjakul.bacterial infections caused by resistant strains have been increased dramatically. pikutbenjakul, a thai medicinal plant formula containing piper longum, piper sarmentosum, piper interruptum, plumbago indica and zingiber officinale have been widely used in thai traditional medicine.201021298838
complete genome sequence of vibrio vulnificus mo6-24/o.vibrio vulnificus is the causative agent of life-threatening septicemia and severe wound infections. here, we announce the complete annotated genome sequence of v. vulnificus mo6-24/o, isolated from a patient with septicemia. when it is compared with previously known v. vulnificus genomes, the genome of this bacterium shows a unique genetic makeup, including phagelike elements, carbohydrate metabolism-related genes, and the superintegron.201121317338
prey bacteria shape the community structure of their predators.although predator-prey interactions among higher organisms have been studied extensively, only few examples are known for microbes other than protists and viruses. among the bacteria, the most studied obligate predators are the bdellovibrio and like organisms (balos) that prey on many other bacteria. in the macroscopical world, both predator and prey influence the population size of the other's community, and may have a role in selection. however, selective pressures among prey and predatory bac ...201121326335
evidence for the role of horizontal transfer in generating pvt1, a large mosaic conjugative plasmid from the clam pathogen, vibrio tapetis.the marine bacterium vibrio tapetis is the causative agent of the brown ring disease, which affects the clam ruditapes philippinarum and causes heavy economic losses in north of europe and in eastern asia. further characterization of v. tapetis isolates showed that all the investigated strains harbored at least one large plasmid. we determined the sequence of the 82,266 bp plasmid pvt1 from the cect4600(t) reference strain and analyzed its genetic content. pvt1 is a mosaic plasmid closely relate ...201121326607
enumeration of vibrio parahaemolyticus in the viable but nonculturable state using direct plate counts and recognition of individual gene fluorescence in situ hybridization.vibrio parahaemolyticus is a gram-negative, halophilic bacterium indigenous to marine and estuarine environments and it is capable of causing food and water-borne illness in humans. it can also cause disease in marine animals, including cultured species. currently, culture-based techniques are used for quantification of v. parahaemolyticus in environmental samples; however, these can be misleading as they fail to detect v. parahaemolyticus in a viable but nonculturable (vbnc) state which leads t ...201121329738
detecting potentially virulent vibrio vulnificus strains in raw oysters by quantitative loop-mediated isothermal amplification.vibrio vulnificus is a leading cause of seafood-related deaths in the united states. sequence variations in the virulence-correlated gene (vcg) have been used to distinguish between clinical and environmental v. vulnificus strains, with a strong association between clinical ones and the c sequence variant (vcgc). in this study, vcgc was selected as the target to design a loop-mediated isothermal amplification (lamp) assay for the rapid, sensitive, specific, and quantitative detection of potentia ...201121357428
tumor necrosis factor-a and mortality in patients infected with vibrio vulnificus.serum tumor necrosis factor-a (tnf-a) was evaluated in vibrio vulnificus-infected patients at admission. the median tnf-a concentration in the non-survivor group was determined to be 261.0 pg/ml, in contrast to 69.5 pg/ml in the survivor group (p = 0.001). hence, serum tnf-a concentration may potentially be an early predictor of the mortality in patients with vibrio septicemia.201121363981
vibrio vulnificus oysters: pearls and perils.vibrio vulnificus is a naturally occurring bacterium found ubiquitously in coastal waters. infection with this organism, which is often associated with eating raw oysters, is the leading cause of seafood-related deaths in the united states. this article reviews the current scientific literature on this potentially lethal pathogen and discusses the prognosis, treatment, and prevention of v. vulnificus infections.201121367733
involvement of the gspab complex in assembly of the type ii secretion system secretin of aeromonas and vibrio species.the type ii secretion system (t2ss) functions as a transport mechanism to translocate proteins from the periplasm to the extracellular environment. the exea homologue in aeromonas hydrophila, gspa(ah), is an atpase that interacts with peptidoglycan and forms an inner membrane complex with the exeb homologue (gspb(ah)). the complex may be required to generate space in the peptidoglycan mesh that is necessary for the transport and assembly of the megadalton-sized exed homologue (gspd(ah)) secretin ...201121378198
bullae and sweat gland necrosis in the differential diagnosis for vibrio vulnificus infection in an alcoholic patient.bullae and sweat gland necrosis remain rare cutaneous manifestation, and these conditions can be misdiagnosed as vibrio vulnificus infections or other soft tissue infections because of their low index of suspicion. a 46-yr-old man with a history of continued alcohol consumption presented with erythematous and hemorrhagic bullous lesions on his left arm. the patient reported that after the ingestion of clams, he slept for 12 hr in a heavily intoxicated state. then the skin lesions started as a re ...201121394318
antimicrobial effects of essential oils of cinnamosma fragrans on the bacterial communities in the rearing water of penaeus monodon larvae.farmed shrimps are vectors of various vibrio species that are considered a potential health hazard. previous study has shown that vibrio parahaemolyticus, vibrio vulnificus, and vibrio alginolyticus dominated in the water and larval samples of shrimp hatchery (randrianarivelo et al. 2010 ). the effects of two essential oils (eos) of cinnamosma fragrans, an endemic plant to madagascar (b8: linalool-type and b143: 1,8-cineole-type), were determined on the total heterotrophic aerobic bacteria and t ...201121395412
structure of a nadph-dependent blue fluorescent protein revealed the unique role of gly176 on the fluorescence enhancement.a nadph-dependent blue fluorescent protein from vibrio vulnificus ckm-1 (bfpvv) emits blue fluorescence under uv-exposure. previously, the bfpvvd7 mutant generated by directed evolution displayed a fourfold enhancement in fluorescent intensity. herein, a further increase in fluorescence in the new bfpvvd8 mutant, with three additional mutations from bfpvvd7, was made. to understand the underlying mechanism of the increased fluorescent intensity of bfpvv, we solved the bfpvvd8-nadph complex struc ...201121397029
regulation of cytotoxicity by quorum-sensing signaling in vibrio vulnificus is mediated by smcr, a repressor of hlyu.cytotoxicity is an important virulence determinant in the pathogenesis of vibrio vulnificus, and two cytotoxins, rtx (encoded by rtxa1) and cytolysin/hemolysin (encoded by vvha), have been identified in this organism. we showed that the quorum-sensing regulator luxo controlled the cytotoxicity of this organism: a +öluxo mutant exhibited low cytotoxicity, whereas a constitutively activated luxo mutant, luxo(d47e), remained highly cytotoxic. the cytotoxicity of the +öluxo mutant was restored when ...201121398530
high intragenomic heterogeneity of 16s rrna genes in a subset of vibrio vulnificus strains from the western mediterranean coast.heterogeneity among ribosomal operons in vibrio vulnificus is purported as a probabilistic indicator of strain virulence and classifies v. vulnificus strains as 16s rrna genes type a and b. in this study, 16s rrna genes typing of v. vulnificus strains isolated from the valencia city coast, in the western mediterranean, showed that 24 out of 30 isolates were type a, one was type b and five could not be typed. single strand conformation polymorphism (sscp) analysis of this gene region revealed com ...201021404212
rtx toxin enhances the survival of vibrio vulnificus during infection by protecting the organism from phagocytosis.vibrio vulnificus is a marine bacterium causing serious septicemia and wound infection in humans. it produces an rtx toxin that can lyse a variety of cells and is important for virulence in mice. in this study, we explored the role of rtx in pathogenesis by characterizing an rtx-deficient mutant. this mutant showed an ~2-log reduction in virulence for mice infected by various routes. survival of the mutant at the infection site and subsequent spread into the bloodstream were impaired. in mice pr ...201121422475
inactivation of vibrio vulnificus hemolysin through mutation of the n- or c-terminus of the lectin-like domain.vibrio vulnificus is an etiological agent causing serious systemic infections in the immunocompromised humans or cultured eels. this species commonly produces a hemolytic toxin consisting of the cytolysin domain and the lectin-like domain. for hemolysis, the lectin-like domain specifically binds to cholesterol in the erythrocyte membrane, and to form a hollow oligomer, the toxin is subsequently assembled on the membrane. the cytolysin domain is essential for the process to form the oligomer. thr ...201121426913
in-vitro antagonistic characteristics of crude aqueous and methanolic extracts of garcinia kola (heckel) seeds against some vibrio bacteria.the methanolic and aqueous extracts of garcinia kola seeds were screened for their anti-vibrio activities against 50 vibrio isolates obtained from wastewater final effluents in the eastern cape province, south africa. the crude extracts at 10 mg/ml exhibited appreciable inhibitory activities against most of the test vibrio isolates, with zones of inhibition ranging from 10-19 mm for methanol extract and 8-15 mm for the aqueous extracts. the minimum inhibitory concentrations (mic) of the methanol ...201121441874
dynamic analysis of pathogen-infected host cells using quantitative phase microscopy.we present the real-time quantitative analysis of vibrio vulnificus-infected host cells using quantitative phase microscopy (qpm) based on interferometric techniques. this provides the ability to retrieve the phase or optical path-length distribution over the cell with nanometer path-length sensitivity from a single interferogram image. we have used qpm to study dynamic cell morphologic changes and to noninvasively quantify the cell volumes of rat basophilic leukemia rbl-2h3 cells infected with ...201121456868
proteases produced by vibrios.bacteria of the genus vibrio are normal habitants of the aquatic environment but the some species are believed to be human pathogens. pathogenic vibrios produce various pathogenic factors, and the proteases are also recognized to play pathogenic roles in the infection: the direct roles by digesting many kinds of host proteins or indirect roles by processing other pathogenic protein factors. especially vvp from vibrio vulnificus is thought to be a major pathogenic factor of the vibrio. although h ...201121467624
factors affecting mortality in hong kong patients with upper limb necrotising fasciitis.to identify predictive factors for mortality of patients with upper limb necrotising fasciitis.201121471588
bacterial pathogens in hawaiian coastal streams-associations with fecal indicators, land cover, and water quality.this work aimed to understand the distribution of five bacterial pathogens in o'ahu coastal streams and relate their presence to microbial indicator concentrations, land cover of the surrounding watersheds, and physical-chemical measures of stream water quality. twenty-two streams were sampled four times (in december and march, before sunrise and at high noon) to capture seasonal and time of day variation. salmonella, campylobacter, staphylococcus aureus, vibrio vulnificus, and v. parahaemolytic ...201121492899
a survey of oysters (crassostrea gigas) in new zealand for vibrio parahaemolyticus and vibrio vulnificus.a microbiological survey was conducted to determine the levels of total and pathogenic vibrio parahaemolyticus (vp) and vibrio vulnificus (vv) in pacific oysters (crassostrea gigas) collected from commercial growing areas in the north island, new zealand. the survey was intended to be geographically representative of commercial growing areas of pacific oysters in new zealand, while selecting the time frame most likely to coincide with the increased abundance of pathogenic vibrio species. vp was ...201121501884
rapid and sensitive detection of vibrio vulnificus by loop-mediated isothermal amplification combined with lateral flow dipstick targeted to rpos gene.a novel loop-mediated isothermal amplification (lamp) combined with amplicon detection by chromatographic lateral flow dipstick (lfd) assay was developed and evaluated for the detection of vibrio vulnificus. biotinylated lamp amplicons were produced by a set of six designed primers that recognized the v. vulnificus rna polymerase subunit sigma factor s (rpos) gene followed by hybridization with an fitc-labeled probe and lfd detection. the optimized time and temperature conditions for the lamp as ...201121513793
comparison of the effects of environmental parameters on growth rates of vibrio vulnificus biotypes i, ii and iii by culture and quantitative pcr.vibrio vulnificus is a natural inhabitant of estuarine waters. the three known biotypes include 1 (most human pathogens), 2 (primarily eel pathogens), and 3 (associated with fish and with human wound infections in israel). despite the frequently lethal consequences of v. vulnificus infections, the growth rates of the various biotypes and their response to environmental changes are not well characterized. we compared the specific growth rates (μ) of a representative of each biotype by culture and ...201121515718
multiplex pcr assay for identification of three major pathogenic vibrio spp., vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus.a multiplex pcr assay was developed based on atpa-sequence diversification for molecular identification of 3 major pathogenic vibrio species: vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus. it specifically identified them from among 133 strains of various vibrio species and other genera, and was applicable for testing seawater, suggesting its usefulness.201121530641
modulation of iron-uptake systems by a mutation of luxs encoding an autoinducer-2 synthase in vibrio vulnificus.vibrio vulnificus possesses multiple iron-uptake systems which are mediated by vuua (vulnibactin receptor), iuta (aerobactin receptor) and hupa (heme receptor). in this study, we determined the effect of a mutation of luxs encoding autoinducer-2 (ai-2) synthase on the expressions of the three receptors. a mutation and an in trans complementation of luxs did not affect the growing ability of v. vulnificus in iron-deficient conditions. nevertheless, the luxs mutation slightly decreased vuua expres ...201121532149
inactivation kinetics of vibrio vulnificus in phosphate-buffered saline at different freezing and storage temperatures and times.vibrio vulnificus (vv) is a pathogen that can be found in raw oysters. freezing can reduce vv and increase the shelf life of oysters. the objective of this study was to develop predictive inactivation kinetic models for pure cultures of vv at different frozen storage temperatures and times. vv was diluted in phosphate-buffered saline (pbs) to obtain about 10(7) cfu/ml. samples were frozen at -10, -35, and -80 °c (different freezing rates), and stored at different temperatures. survival of vv was ...201121535763
the pseudomonas quinolone signal (pqs), and its precursor hhq, modulate interspecies and interkingdom behaviour.the pseudomonas quinolone signal (pqs), and its precursor 2-heptyl-4-quinolone (hhq), play a key role in coordinating virulence in the important cystic fibrosis pathogen pseudomonas aeruginosa. the discovery of hhq analogues in burkholderia and other microorganisms led us to investigate the possiblity that these compounds can influence interspecies behaviour. we found that surface-associated phenotypes were repressed in gram-positive and gram-negative bacteria as well as in pathogenic yeast in r ...201121539583
vibrio parahemolyticus septicaemia in a liver transplant patient: a case report.abstract: introduction: vibrio parahemolyticus is the leading cause of vibrio-associated gastroenteritis in the united states of america, usually related to poor food handling; only rarely has it been reported to cause serious infections including sepsis and soft tissue infections. in contrast, vibrio vulnificus is a well-known cause of septicaemia, especially in patients with cirrhosis. we present a patient with v. parahemolyticus sepsis who had an orthotic liver transplant in 2007 and was on i ...201121548914
a conserved gtpase ychf of vibrio vulnificus is involved in macrophage cytotoxicity, iron acquisition, and mouse virulence.vibrio vulnificus, a highly virulent marine bacterium, causes serious wound infections and fatal septicemia in many areas of the world. to identify v. vulnificus genes required for killing macrophages, we made an insertional mutant library of v. vulnificus and screened it for reduced macrophage cytotoxicity. one mutant defective in macrophage cytotoxicity had an insertion in ychf, a gene encoding a putative gtpase. in addition to reduced cytotoxicity, this mutant had attenuated growth in iron-li ...201121570909
[development of an immunochromatographic strip test for rapid detection of vibrio vulnificus.]objective: to establish a rapid strip test for detection of vibrio vulnificus. method: anti-vibrio vulnificus polyclonal antibodies were obtained from the rabbits immunized with vibrio vulnificus (atcc27562). colloidal gold was prepared through reducing haucl(4)·4h(2)o by sodium citrate and conjugated with the polyclonal antibodies as the detecting reagent. the polyclonal antibodies and sheep anti-rabbit immunoglobulins were separately coated onto the same nitrocellulose membrane for sample dete ...201121602152
frsa functions as a cofactor-independent decarboxylase to control metabolic flux.the interaction between fermentation-respiration switch (frsa) protein and glucose-specific enzyme iia(glc) increases glucose fermentation under oxygen-limited conditions. we show that frsa converts pyruvate to acetaldehyde and carbon dioxide in a cofactor-independent manner and that its pyruvate decarboxylation activity is enhanced by the dephosphorylated form of iia(glc) (d-iia(glc)). crystal structures of frsa and its complex with d-iia(glc) revealed residues required for catalysis as well as ...201121623357
transgenic expression of tilapia hepcidin 1-5 and shrimp chelonianin in zebrafish and their resistance to bacterial pathogens.recently, tilapia hepcidin (th)1-5 was characterized, and its antimicrobial functions against several pathogens were reported. the antimicrobial functions of another shrimp antimicrobial peptide (amp), chelonianin, were also characterized using a recombinant chelonianin protein (rcf) that was expressed by a stably transfected chinese hamster ovary (cho) cell line against pathogen infections in fish. the function of the overexpression of both amps in zebrafish muscles was not examined in previous ...201121642002
temporal and spatial variability in culturable pathogenic vibrio spp. in lake pontchartrain, louisiana, usa, following hurricanes katrina and rita.we investigated the abundance, distribution, and virulence gene content of v. cholerae, v. parahaemolyticus, v. vulnificus in the waters of southern lake pontchartrain in louisiana, usa, on four occasions from october 2005 to september 2006, using selective cultivation and molecular assays. the three, targeted pathogenic vibrios were generally below detection in january 2006 when the water was cold (13° c), and highest in september 2006 when lake water was warmest (30° c). the maximum values for ...201121642406
identification and characterization of a novel serine protease vvps containing two functional domains and essential for autolysis of vibrio vulnificus.little is known about the molecular mechanism for autolysis of gram (-) bacteria. in the present study, we identified the vvps gene encoding a serine protease, vvps, from vibrio vulnificus, a gram (-) food-born pathogen. the amino acid sequence predicted that vvps consists of two functional domains, an n-terminal protease catalytic domain (pcd) and c-terminal carbohydrate binding domain (cbd). a null mutation of vvps significantly enhanced viability during stationary phase, as measured by enumer ...201121642466
design and validation of a novel multiplex real-time pcr assay for vibrio pathogen detection.three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus--account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species. additionally, biochemical identification and confirmation require 2 or more days to complete. rapid and sensitive molecular techniques for the detection of vibrio pathogens would be useful for the surveillance and management o ...201121669071
chitin-induced carbotype conversion in vibrio vulnificus.as an etiological agent of bacterial sepsis and wound infections, vibrio vulnificus is unique among the vibrionaceae. the most intensely studied of its virulence factors is the capsular polysaccharide (cps). over 100 cps types have been identified, yet little is known about the genetic mechanisms that drive such diversity. chitin, the second-most-abundant polysaccharide in nature, is known to induce competence in vibrio species. here, we show that the frequency of chitin-induced transformation i ...201121670169
occurrence and potential pathogenesis of vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus on the south coast of sweden.during the summer of 2006, several wound infections - of which three were fatal - caused by vibrio cholerae were reported from patients who had been exposed to water from the baltic sea. before these reports, we initiated a sampling project investigating the occurrence of potential human pathogenic v. cholerae, vibrio vulnificus and vibrio parahaemolyticus in the sound between sweden and denmark. the blue mussel (mytilus edulis) was used as an indicator to follow the occurrence of vibrios over t ...201121692819
intranasal immunization with recombinant pspa fused with a flagellin enhances cross-protective immunity against streptococcus pneumoniae infection in mice.streptococcus pneumoniae is a major respiratory pathogen that causes high levels of mortality and morbidity in infants and the elderly. despite the use of antibiotics and vaccines, fatal pneumococcal disease remains prevalent. pneumococcal surface protein a (pspa), a highly immunogenic surface protein produced by all strains of s. pneumoniae, can elicit protective immunity against fatal pneumococcal infection. we have previously demonstrated that the vibrio vulnificus flab, a bacterial flagellin ...201121696869
genomic and metabolic profiling of nonulosonic acids in vibrionaceae reveal biochemical phenotypes of allelic divergence in vibrio vulnificus.nonulosonic acids (nulos) encompass a large group of structurally diverse nine-carbon backbone +¦-keto sugars widely distributed among the three domains of life. mammals express a specialized version of nulos called sialic acids, which are displayed in prominent terminal positions of cell surface and secreted glycoconjugates. within bacteria, the ability to synthesize nulos has been demonstrated in a number of human pathogens and is phylogenetically widespread. here we examine the distribution, ...201121724895
comparative analysis of proteins in the culture supernatants of human intestinal epithelial cells infected with the wild-type and rtxe mutant of vibrio vulnificus.bacterial virulence factors and secreted extracellular proteins from damaged host cells following infection have been recognized as key mediators in the pathophysiological alterations observed in septic shock, and have also been shown to have a synergistic influence on bacterial pathogenicity. we hypothesized that during infections, virulence factors as well as host-shed proteins may synergistically influence aspects of the pathogenicity of v.-ávulnificus, such as primary septic shock and overpr ...201121725587
Vibrio virulence genes in fishes collected from estuarine waters in Italy.Aims:ÔÇé The aim of this study was to investigate the presence of Vibrio vulnificus and potentially pathogenic strains of Vibrio parahaemolyticus in mullets collected from estuarine environment in Italy. Methods and Results:ÔÇé Two hundred and ninety-five mullets were analysed by culture using the selective medium thiosulfate citrate bile salt sucrose agar, during a monitoring period of 2ÔÇâyears (2008-2009). Presumptive Vibrio colonies were initially identified by using biochemical tests, and s ...201121740448
insights into the antibacterial and immunomodulatory functions of tilapia hepcidin (th)2-3 against vibrio vulnificus infection in mice.the antimicrobial and immunomodulatory functions of the antimicrobial peptide, tilapia hepcidin (th)2-3, against a bacterial endotoxin under in vitro conditions was previously reported. in this study, we investigated the antibacterial and immunomodulatory functions of th2-3 in mice infected with the pathogen, vibrio vulnificus. a th2-3 injection in v. vulnificus-infected mice produced an increased survival rate compared to mice injected with v. vulnificus only. in addition, a th2-3 injection inc ...201121756935
a new leukocyte cell-derived chemotaxin-2 from marine fish grouper, epinephelus coioides: molecular cloning and expression analysis.leukocyte cell-derived chemotaxin-2 (lect2) is a multifunctional protein involved in cell growth, differentiation and autoimmunity. in this study, a new leukocyte cell-derived chemotaxin-2 (eclect2) gene was cloned from grouper, epinephelus coioides, by rapid amplification of cdna ends (race) pcr. the full-length cdna sequence of eclect2 was 595 bp in size, containing a 5'-untranslated region (utr) of 44 bp and a 3'-utr of 83 bp. the deduced protein sequence of the open reading frame (465 bp) sh ...201121763428
functional characterization of epsc, a component of the type ii secretion system, in the pathogenicity of vibrio vulnificus.epsc, one of the components comprising the type ii secretion system (t2ss), was isolated from a human pathogenic bacterium, vibrio vulnificus, to evaluate its role in eliciting virulence. an espc-deleted mutant v. vulnificus displayed a reduced cytotoxicity to the human cell line hep-2 and an attenuated virulence in a mouse model. this mutant exhibited dramatic defects in secretion of diverse extracellular proteins, such as outermembrane proteins, transporters, and the known secreted factors, no ...201121788383
necrotising fasciitis caused by vibrio vulnificus in the lower limb following exposure to seafood on the hand.vibrio vulnificus infection mainly manifests as primary bacteraemia or gastroenteritis following injection of the microorganism, and wound infection through direct inoculation. injury from exposure to the microorganism and development of necrotising fasciitis at a remote site and sepsis are rare. this report is of a high-risk patient with haemoglobin h disease who developed necrotising fasciitis in the lower limb after exposure to seafood on the hand.201121813906
the antifouling potentiality of galactosamine characterized from vibrio vulnificus exopolysaccharide.to gain a better insight into biofilm composition, the exopolysaccharide (eps) of the gram-negative bacterium vibrio vulnificus was studied. monosaccharide composition analysis of the wild-type and mutant v. vulnificus eps carried out with bio-liquid chromatography revealed the presence of d-glucosamine, d-galactose, d-glucose and d-xylose in both strains. d-galactosamine was found only in the mutant that formed less biofilm compared to its wild-type. the influence of galactosamine on biofilm fo ...201121827336
influence of iron-chelated growth conditions on outer membrane protein production and virulence of vibrio tubiashii.growth of two vibrio tubiashii strains under iron-chelated conditions resulted in the production of a hydroxymate-like siderophore, and expression of outer membrane proteins with homologies to proteins in vibrio cholerae and vibrio vulnificus which were not seen in cells grown under non-chelated growth conditions. pcr analysis using primers based on listonella anguillarum's ferric uptake repressor protein (fur) gene detected a 316-ábp fur gene homolog which also had sequence homology to the fur ...201121839393
Vibrio vulnificus Diarrhea in a Child with Respiratory Infection.Vibrio vulnificus is a rare cause of disease and it is often unrecognized and underreported. It is a lactose-fermenting, halophilic vibrio causing severe disease in immunocompromised patients, but causing a mild type of gastroenteritis in healthy people, usually associated with consumption of seafood. We report here a case of diarrhea due to V. vulnificus in a male child who was admitted for fever, loose motions and productive cough. There was no history of consumption of any seafood; so, the so ...201121887066
Overlapping and unique contributions of two conserved polysaccharide loci in governing distinct survival phenotypes in Vibrio vulnificus.As an aetiological agent of bacterial sepsis and wound infections, Vibrio vulnificus is unique among the Vibrionacea. Its continued environmental persistence and transmission are bolstered by its ability to colonize shellfish and form biofilms on various marine biotic surfaces. We previously identified a polysaccharide locus, brp, which contributes to the survival phenotypes of biofilm formation, rugose colony formation and stress resistance. Here, we describe a second polysaccharide locus, rbd ...201121895917
Prevalence and antimicrobial susceptibility of major foodborne pathogens in imported seafood.Seafood is a leading commodity implicated in foodborne disease outbreaks in the United States. Seafood importation rose dramatically in the past 3 decades and now contributes to more than 80% of the total U.S. seafood supply. However, limited data are available on the microbiological safety of imported seafood. In this study, we obtained a total of 171 salmon, shrimp, and tilapia samples imported from 12 countries in three retail stores in Baton Rouge, LA. The total microbial population and the ...201121902913
Vibrio vulnificus IlpA induces MAPK-mediated cytokine production via TLR1/2 activation in THP-1 cells, a human monocytic cell line.Vibrio vulnificus is a pathogenic bacterium causing primary septicemia, which is followed by a classical septic shock pathway including an overwhelming inflammatory cytokine response. V. vulnificus IlpA is a potent immunogenic lipoprotein that triggers cytokine production in human monocytes by activating the toll-like receptor 2 (TLR2). In this study, we further defined the IlpA signaling pathways involved in cytokine production in the human monocytic cell line, THP-1. TLR2 was involved in cytok ...201121903273
[preparation and identification of ompw monoclonal antibodies].to prepare and characterize the mouse monoclonal antibodies against vibrio parahaemolyticus ompw.201121906472
Refresh
firstprev
...313233343536373839
nextlast
Displaying items 3201 - 3300 of 3837