Publications

antiviral chemotherapy and prophylaxis of viral respiratory disease.	respiratory viruses continue to be major causes of morbidity and mortality. currently available chemotherapy is limited to oral amantadine for uncomplicated influenza a and aerosolized ribavirin for respiratory syncytial virus (rsv) infections. amantadine is also efficacious for chemoprophylaxis of influenza a virus infections. rimantadine has similar clinical efficacy and is better tolerated than amantadine. aerosolized ribavirin may be useful in the treatment of serious respiratory illness cau ...	1987	2446820
theophylline's inhibition of antigen-induced contraction of human parenchymal strips is independent of adenosine antagonism.	the effects of theophylline, and adenosine antagonist, and enprofylline, reported to lack adenosine antagonism, were studied using antigen-induced contraction of sensitized human lung parenchymal strips. cumulative addition of adenosine usually resulted in a small concentration-dependent contraction, which was antagonized by theophylline, 100 microm. antigen-induced contraction was reduced by pretreatment with adenosine, 10 microm, but not by adenosine addition after antigen challenge. dipyridam ...	1987	2446888
peripheral blood admixture in bone marrow aspirates.	a simple method is described by which the degree to which a bone marrow aspirate is diluted with peripheral blood, the admixture of peripheral blood leukocytes in the aspirate, and the true cellularity of bone marrow at the aspiration site can be estimated quantitatively. a highly significant correlation is shown to exist between the true bone marrow cellularity and the number of hemopoietic cells in the bone marrow aspirate. this correlation can be described by an exponential regression equatio ...	2008	2446995
inhibition of allergic encephalomyelitis by the iron chelating agent desferrioxamine: differential effect depending on type of sensitizing encephalitogen.	induction of experimental allergic encephalomyelitis (eae) in lewis rats by injection of guinea pig (gp) spinal cord homogenate (sch) plus adjuvant (sch-cfa) can be inhibited by treatment with the iron chelating agent desferrioxamine (dfom). interestingly, induction of eae with purified myelin basic protein (bp-cfa) is not inhibited with dfom. this dichotomy does not appear to be due to any quantitative differences in the two inocula since minimal clinical eae produced by threshold levels of bp ...	1988	2447124
[social psychiatry in private practice].	though in general the practice of community mental health (or "social psychiatry") is a task of the state, the county or the city governments, the panel discussed two instances of sizeable contributions by private practices to the tasks of community mental health, such as crisis intervention or the care of the chronically psychiatrically handicapped, which are not commonly treated in psychiatrists' offices. the "psychosoziale arbeitsgemeinschaft" of basle began in a psychiatric group practice th ...	2008	2447644
structural homology of human complement component c8 gamma and plasma protein hc: identity of the cysteine bond pattern.	anti-c8 alpha-gamma specific antibodies were used to isolate cdna clones from a human liver expression library. antibodies affinity-purified on the expressed hybrid protein of one clone bound exclusively to the gamma-chain of reduced c8 alpha-gamma. this clone, as well as a second full length cdna clone obtained by hybridization screening, were sequenced and the complete primary structure for c8 gamma was established. cyanogen bromide cleavage of c8 alpha-gamma released a 12 kda carboxy-terminal ...	1987	2447883
shiga toxin inhibits the anti-invasive effect of interferons.		1988	2450154
[relation of the immunosuppressivity of virulent shigella to the structure of o antigen].	the capacity of filtrates of virulent shigella cultures (vsc) and their different fractions to render avirulent strains, injected intraperitoneally into mice simultaneously with these filtrates, capable of suppressing immune response (delayed hypersensitivity) has been studied. among the fractions of vsc filtrates, the lipoid fraction soluble in the mixture of ethanol and ether has proved to be active. active factors can be extracted from vsc filtrates by means of immunosorbents obtained from an ...	1988	2452538
high-yield purification of a pp60c-src related protein-tyrosine kinase from human platelets.	a protein-tyrosine kinase (ptk, ec 2.7.1.112) from human platelets was purified with high yield. purification of the enzyme involved sequential chromatography on casein-agarose, tyrosine-agarose, heparin-sepharose and hydroxylapatite. the procedure resulted in substantially enriched 54/52 kda polypeptides on sds-polyacrylamide gel electrophoresis and a yield of about 25% in ptk activity. about 250 micrograms of purified protein could be obtained from 1 g of cell protein. the purification factor ...	1988	2453218
natural killer cell activation and interferon production by peripheral blood lymphocytes after exposure to bacteria.	we have previously shown that peripheral blood natural killer (nk) cells have significant levels of cytotoxic activity against shigella flexneri-infected hela cells. in this report, we show that nk cell activity against k562 tumor cells and shigella flexneri-infected hela cells can be greatly enhanced by preincubating peripheral blood lymphocytes (pbl) for 18 h with kanamycin-treated shigella flexneri or salmonella typhimurium. cell-free supernatants obtained from pbl-bacteria cultures contained ...	1988	2453465
[current approaches to the diagnosis of immunopathologic conditions].		2008	2453710
activation of the mas oncogene during transfection of monoblastic cell line dna.	using the tumorigenicity assay, we observed an activation of the mas oncogene during transfection of human acute myelocytic leukemia (ctv-2) dna. respective transfectants contained amplified mas sequences characterized by rearrangements in 5' and 3' noncoding regions that were transcribed into a 3.3 kb mas rna species. our data represent the second report on activation of the mas gene.	1988	2453759
structural and immunochemical studies of the lipopolysaccharide from a new provisional serotype of shigella flexneri.	the chemical structure of the o-antigen of a proposed new provisional serotype of shigella flexneri has been determined. methylation analysis, glc-ms, 1h-nmr and 13c-nmr showed that the linear o-antigenic polysaccharide is the same as for all s. flexneri [kenne, l., lindberg, b., petersson, k. & romanowska, e. (1977) carbohydr. res. 56, 363-370]. a novel structural feature is that the disaccharide alpha-d-glcp-(1----2)-alpha-d-glcp is linked to o4 of the n-acetyl-glucosamine residue. (formula: s ...	1988	2458260
shigella flexneri invasion plasmid antigens b and c: epitope location and characterization with monoclonal antibodies.	invasion plasmid antigens b (ipab) and c (ipac) are associated with the ability of shigellae to invade cultured mammalian cells. monoclonal antibodies against ipab and ipac polypeptides were produced and used in a whole-cell enzyme-linked immunosorbent assay to show that both ipab and ipac polypeptides were exposed on the surface of virulent shigellae. moreover, these surface epitopes were shown to be highly conserved among different serotypes of shigella spp. and enteroinvasive escherichia coli ...	1988	2459066
bacterial epitopes involved in the induction of reactive arthritis.	a panel of documented arthritogenic shigella flexneri strains as well as an epidemic-associated non-arthritogenic shigella sonnei control was used to identify a 2-megadalton plasmid specific to the arthritogenic strains. the plasmid, phs-2, contains a dna sequence that encodes a 22-amino acid polypeptide encompassing a pentapeptide homologous to part of the polymorphic region of the alpha-1 domain of hla-b27. these results suggest that molecular mimicry between arthritogenic bacterial-encoded ep ...	1988	2462352
immunoglobulin m, a, and g antibody response to lipopolysaccharide o antigen in symptomatic and asymptomatic shigella infections.	the antilipopolysaccharide antibody response in sera obtained from subjects involved in 10 outbreaks of shigellosis occurring in israeli military field units was determined by an enzyme-linked immunosorbent assay and a passive hemagglutination test. both tests were found to be sensitive and specific for the diagnosis of shigellosis. a significant antibody response was detected in 73 to 82% of the symptomatic and 48 to 60% of the asymptomatic subjects during the shigella sonnei and shigella flexn ...	1989	2463995
[specificity of the protective action of a ribosomal shigella vaccine and the absence of activity in the ribosomes from r mutants].	the ribosomal preparations of s. sonnei and some other bacterial species were obtained by the method of differential centrifugation, and the specificity of their protective action was studied in the keratoconjunctivitis test on guinea pigs. the ribosomal preparations were introduced parenterally in a single injection, and their protective action was determined two weeks later by the challenge of the animals with s. sonnei virulent strain and the subsequent calculation of the efficiency index (ei ...	1988	2464261
[lipid peroxidation in experimental viral infection and the effect on it of interferons and alpha-tocopherol].	lipid peroxidation as well as effects of various interferons (native, from fibroblasts of new born and adult animals) on the peroxidation were studied in blood plasma, erythrocytes, liver and kidney tissues of mice with viral infection. these interferons, simultaneously with antiviral action, exhibited the positive effect on lipid peroxidation, while the interferon from fibroblasts of newborns produced the side reactions. simultaneous use of the interferon and alpha-tocopherol removed these side ...	1988	2467439
[comparative diagnostic value of methods for detecting dysentery antigens in substrates of the patient's body].	the comparative evaluation of different immunological methods, such as the enzyme immunoassay, the aggregate hemagglutination test and the complement fixation test, used for the detection of specific shigella antigens in biological body substrates obtained from 287 patients with acute dysentery caused by s. sonnei, s. flexneri and s. newcastle has been carried out. the enzyme immunoassay and the aggregate hemagglutination test most effective (97.5 +/- 0.5 and 92.4 +/- 0.9, respectively), the obj ...	1989	2469267
feeding by mucin and intestinal growth of some enteric bacterial pathogens.	enteroinvasive escherichia coli, salmonella typhi-murium, shigella sonnei, shigella flexneri, as well as e. coli k-12 show dose dependent growth in minimal medium completed with purified hog gastric "granular mucin". this ability is based on alpha-galactosidase production: defective, melibiose (and galactose) non-fermenting k-12 mutant were unable to utilize mucin. the viability of the parent k-12 strain in the cecal content of mice is significantly higher than that of its mel- mutant phenotype. ...	1988	2469280
human milk immunoglobulin a antibodies to shigella virulence determinants.	because human milk is thought to protect infants from shigellosis, we evaluated milk for immunoglobulin a to shigella virulence determinants. milk was preincubated to remove antibodies unrelated to each locus of interest, using defined shigella and e. coli hybrids containing known shigella genetic segments prior to immunoblotting. the milk could not be shown to contain antibodies to chromosomally encoded virulence loci except for the expected antibodies to the products of the histidine locus. ho ...	1989	2470676
in vitro treatment of hep-2 cells with human tumor necrosis factor-alpha and human interferons reduces invasiveness of salmonella typhimurium.	enteroinvasive bacteria, like salmonella typhimurium, can be internalized in in vitro cultured epithelioidal cells, like hep-2 cells. this phenomenon is inhibited by pretreatment of cells with human tumor necrosis factor alpha (tnf-alpha) in a dose- and time-dependent manner. the effect was also reproduced in other cell types, including diploid embryo fibroblast cells. the tnf-alpha effect was neutralized by anti-tnf-alpha antibodies. no synergistic effect was produced by combinations of tnf-alp ...	1989	2472042
expression in escherichia coli k-12 of the 76,000-dalton iron-regulated outer membrane protein of shigella flexneri confers sensitivity to cloacin df13 in the absence of shigella o antigen.	one of the chromosomal segments associated with virulence in shigella flexneri encodes the production of aerobactin and the synthesis of an iron-regulated 76-kilodalton outer membrane protein believed to be the ferric-aerobactin receptor. however, s. flexneri expressing this putative aerobactin receptor, which is slightly larger than that encoded by pcolv, is insensitive to the killing action of cloacin df13, a bacteriocin which binds to other aerobactin receptor proteins and kills the cells. in ...	1989	2474501
a dual transcriptional activation system for the 230 kb plasmid genes coding for virulence-associated antigens of shigella flexneri.	the expression of plasmid-encoded, invasion-related antigens lpa b, c and d of shigella flexneri was found to be positively regulated at transcriptional level by a 33kd protein produced by the previously defined, virulence-associated region 1 on the sali fragment b of the 230 kb invasion plasmid. the gene (designated virb) was identified and its nucleotide sequence determined. no ipa b or c was produced in the absence of an intact virb gene although lower levels of d were produced. the previousl ...	1989	2474742
identification of a 2-md plasmid from shigella flexneri associated with reactive arthritis.	the development of reactive arthritis, a sterile inflammatory polyarthropathy that primarily affects hla-b27 positive individuals, has been associated with previous enteric infections caused by various gram-negative bacteria. the possibility that a common bacterial epitope triggers the disease was investigated by screening a panel of documented arthritogenic shigella strains as well as 2 epidemic-associated nonarthritogenic shigella controls. a 2-md plasmid specific to the arthritogenic strains ...	1989	2475114
identification and characterization of transcription termination sites in the escherichia coli lacz gene.	the escherichia coli lacz gene contains a series of latent transcriptional terminators that are responsible for the polar effects of certain mutations. we demonstrate, using gel electrophoretic size analyses and nuclease s1 mapping procedures, that rna polymerase terminates rna synthesis in the vicinity of five positions 180, 220, 379, 421 and 463 base-pairs downstream from the start point during transcription of lacz dna in vitro in the presence of rho factor. termination at all but the 421 pos ...	1989	2475637
antihypertensive, anticalcinotic, and antiarteriosclerotic properties of anipamil, a long-acting new derivative of verapamil.	the classical indication for calcium antagonists has for a long time been coronary heart disease. however, more recent experimental data as well as a host of clinical observations now encourage a broader use of these drugs also for antihypertensive purposes and for prevention of concomitant sclerotic vascular injury. needless to say, for the treatment of hypertensive crises rapidly acting calcium antagonists are required, whereas for chronic therapy of hypertension calcium antagonists with a lon ...	1989	2475679
[development and use of solid-phase test-systems for the detection of o-antigen of shigella sonnei and shigella flexneri].	the possibility of the diagnosis of dysentery caused by s. sonnei and s. flexneri, as well as the determination of the dynamics of the distribution of specific o-antigen in the patient's body, by means of the enzyme-linked immunosorbent assay system developed on the basis of antibody preparations obtained by immunosorption has been studied. the study has shown that for better diagnosis the use of fecal extracts is preferable in assays; when used in combination with bacteriological analysis, thes ...	1989	2477970
[use of immunoenzyme analysis in the rapid diagnosis and study of the characteristics of circulating o-antigen in biological fluids of patients with acute dysentery sonne].	the data on the clinical approval of the original enzyme immunoassay system for the determination of somatic o-antigen in the blood serum and urine of patients with acute sonne dysentery are presented. the level of the antigen determined in the biological fluids of patients has been shown to depend on the severity of the disease. different types of dynamic curves, reflecting the level of o-antigen in the biological fluids of patients with acute sonne dysentery and characteristic of different cli ...	1989	2477971
binding protein, radioreceptor and biological activities of recombinant methionyl insulin-like growth factor-i variants.	reversed-phase chromatography (rpc) was used to resolve two variants of recombinant amino terminal methionyl residue (n-met) insulin-like growth factor-i (igf-i) with the same amino acid constitution but different disulphide linkages. following radioiodination, equilibration with plasma and size exclusion chromatography at neutral ph the major form on rpc (approximate abundance 60%) demonstrated greater than 80% binding to 150 kda and 40-50 kda igf binding proteins. this peptide has the rpc elut ...	2008	2479583
prostatic "dysplasia/atypical hyperplasia". terminology, histopathology, pathobiology, and significance.		2008	2481361
[cloning of the gene relative to invasive ability of shigella flexneri 5].	a genomic library of s. flexneri 5 large plasmid (140 md) was constructed using the cosmid pjb8 as vector. there were more than 4,000 transformants in the library. 66 clones were picked out from the library by hybridization with 17kb gene probe relative to invasive ability. a few clones were analyzed. the results indicated that all these clones tested contained recombinant plasmids, they could hybridize with 17 kb gene probe. when these recombinant plasmids were digested with ecor1, 17 kb fragme ...	1989	2486252
mechanism of antigenic variation in shigella flexneri bacilli. i. selective lethal effect of normal sera on mixed population of s. flexneri 1b serotype and its antigenic 3b mutant.	normal non-immune calf, human and mice sera kill-preferentially the shigella flexneri 1b serotype cells with antigenic formula i; 3, 4, 6 leaving the antigenic mutant cells of the serotype 3b with antigenic formula iii; 3, 4, 6. the strongest lethal action was observed in normal calf serum. this serum makes possible to obtain the 3b serotypes mutant from pure population of 1b serotype cells. thermal inactivation of the complement deprives the serum of its selective activity.	1989	2487379
lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats.		1989	2492809
intracisternal injection of a trh analogue stimulates gastric luminal serotonin release in rats.	intracisternal injection of trh analogue, rx 77368, which has been previously shown to induce a vagal-dependent stimulation of gastric acid secretion and contractility dose-dependently (10-300 ng) stimulated serotonin release into the gastric lumen of conscious pylorus-ligated rats. the stimulation of gastric acid and serotonin release occurred 15 and 30 min, respectively, after intracisternal injection of rx 77368 (100 ng) and lasted for over 2 h, as measured in urethan-anesthetized rats with g ...	1989	2493198
renal function and morphology in sudanese patients with advanced hepatosplenic schistosomiasis and portal hypertension.	the association between glomerular disease and hepatosplenic schistosomiasis is well documented in reports from south america. during the present hospital investigation in sudan, 58 patients admitted for intercurrent complications of advanced hepatosplenic schistosomiasis were studied. the patients, median age 35 years, had no concurrent schistosoma haematobium infection. diagnostic criteria included an enlarged spleen (n = 58), at least 1 episode of hematemesis (n = 55) and/or melena (n = 36), ...	1989	2493202
in vitro susceptibility of feline herpesvirus-1 to vidarabine, idoxuridine, trifluridine, acyclovir, or bromovinyldeoxyuridine.	in vitro activities of 9-[( 2-hydroxyethoxy] methyl) guanine (acyclovir), (e)-5-(2-bromovinyl)-2'deoxyuridine, 9-beta-d-arabinofuranosyladenine (vidarabine), 5-iodo-2'-deoxyuridine (idoxuridine), and 5-trifluoromethyl-2'-deoxyuridine (trifluridine) were studied against 6 strains of feline herpesvirus-1. a significant difference was not detected among viral strains in their susceptibility to these compounds (p = 0.442). the relative potency of these compounds was trifluridine much greater than id ...	1989	2493210
ambulatory management of suspected deep vein thrombosis.		2008	2493227
'the cost benefit of routine pre-operative hb investigations for oral surgery'.		2008	2493261
[association of sacciform aneurysm of the renal artery and hypertension. cause or coincidence? apropos of a case].	aneurysms of the renal artery are seldom encountered. they present as different aetiopathological types. we report the case of a female patient who had both severe arterial hypertension and an apparently dysplastic aneurysm of the right prepyelic artery. resection of the aneurysm failed to have the effect we expected on arterial hypertension. the relationship between arterial hypertension and aneurysm of the renal artery is discussed.	2008	2494963
general practitioner obstetrics.		2008	2500182
profile of the gmc. 1978 and all that.		2008	2500205
valproate and spina bifida.		2008	2500206
[value of determining the spectrum of blood serum fatty acids in evaluating increasing the effectiveness of antibiotic therapy of dysentery in children with prodigiozan and ephedrine].	one hundred and ninety one children with acute sonne and flexner dysentery were observed with respect to the disease process, immunity indices and blood serum fatty acid spectrum. 104 children were treated with monomycin alone and 87 children were treated with the antibiotic in combination with prodigiozan and ephedrine as immunostimulators. it was shown that the recovery terms in the patients treated with the use of the immunostimulators decreased as compared to the patients treated with the an ...	1989	2502081
hemagglutinating properties of shigella dysenteriae type 1 and other shigella species.	strains of shigella dysenteriae type 1 cultured in casamino acids-yeast extract broth medium in the presence of 1 mm calcium chloride at 37 degrees c for 22 h induced hemagglutination of erythrocytes that was inhibited by n-acetylneuraminic acid, n-acetylneuramin-lactose, and alpha 1-glycoprotein. the hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). under the same conditions, strains of shigella flexneri (types 1a, 1b, 2a, and 2b) s ...	1989	2503449
infections in british clinical laboratories, 1986-87.	during 1986-87 this continuing survey showed 15 specific infections in the staff of 235 laboratories, representing 28,524 person years of exposure. the community was the probable source of four of the five cases of tuberculosis and one of the five cases of salmonellosis. occupational exposure was the probable cause of four infections by shigella flexneri, three by salmonella typhimurium, and one by s typhi, all affecting medical laboratory scientific officers (mlsos) in microbiology. occupationa ...	1989	2503546
[clinical studies on patients with legal communicable diseases encountered from 1981 to 1986].	patients with legal communicable diseases admitted to kawasaki municipal hospital between 1981 and 1986 were studied. ages of patients were over 15 year-old. during this period 115 patients were admitted. out of these patients 84 had shigellosis, and 9 had amebiasis. out of 20 salmonellosis, 18 were due to salmonella typhi and 2 were due to salmonella paratyphi-a, respectively. two were admitted because of cholera. out of the patients with shigellosis, 27 were domestic and 57 were foreign infect ...	1989	2504839
[the isolation and immunologic study of ribosomal preparations from shigella flexneri].	s. flexneri ribosomal preparations were isolated by differential centrifugation or by fractionation with polyethylene glycol-6000. their chemical composition and spectrophotometric properties were characteristic of ribosomes, and, as shown by the results of the serological assay, the content of o-specific component was, on the average, 1.4%. the ribosomal preparations were nontoxic for mice when injected intraperitoneally and intravenously in large doses and induced systemic o-antibody response ...	1989	2511706
[interferon in the treatment of hairy cell leukemia].		2007	2515708
[interferon in the treatment of hairy cell leukemia].		2007	2515708
[possibilities for imaging intracardiac thrombi with indium 111 thrombocyte scintigraphy].	intracardiac thrombi can be localized and quantified by indium-111-labelling of thrombocytes with a high sensitivity and specificity. the scintigraphic procedure has a complementary evidence to echocardiography. scintigraphy shows activity and age of thrombosis, whereas echocardiography seems to be superior in determination of mass and localization. in older thrombi scintigraphy fails because of organisation and endothelialization of the thrombus surface. for the reason of determination of the a ...	2008	2516935
neurological disorders in aids: a study of 18 cases.	neurological disorders were studied in 18 patients diagnosed to have aids and their findings are analysed. amongst the problems seen were toxoplasmosis (9), cryptococcal meningitis (5), tuberculous meningitis (1), aids dementia complex (3), peripheral neuropathy (2), vertebrobasilar stroke, and a possible aids myelopathy in one case each. their findings are discussed, and literature on the neurological disorders in aids reviewed.	2005	2517434
[in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics].	in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ...	1989	2518646
[natural feeding, an important factor in reducing the index of infant mortality].		2008	2518712
[immunoenzymatic determination of free immunoglobulin light chains in the cerebrospinal fluid: its value in the biological diagnosis of multiple sclerosis].	based on previous data on free immunoglobulin light chains in the cerebrospinal fluid (csf) of patients with multiple sclerosis (ms), we evaluated free kappa and lambda light chains in the csf from 3 patients groups: (a) 42 with ms, (b) 16 with other neurological inflammatory diseases (onid) and (c) 42 with non inflammatory neurological diseases (nind) used as control. the kappa and lambda light chains contents were evaluated using a specific and sensitive (0.5 mcg/ml) enzyme linked immunosorben ...	2008	2518740
[in vitro cultivation of human pulpal fibroblast strains--permanent and deciduous teeth].	we succeeded in separating and the cultivating stable monolayer cultures of dental pulp fibroblast strains derived from permanent and deciduous human teeth. human permanent (n = 67) and deciduous teeth (n = 26) were extracted under acupuncture anaesthesia for the correction of malocclusion. after splitting the teeth, the pulp tissues were carefully removed, placed in tissue culture flasks, and grown in dulbecco's modified eagle medium supplemented with 10% fetal calf serum (fcs). the human pulpa ...	1989	2519279
infective endocarditis in children. a seven-year experience.	eighteen children with infective endocarditis were detected over seven years. fever, gastrointestinal and chest symptoms were striking features. anaemia, elevated leukocyte count and sedimentation rate were constant laboratory findings. strep. viridans and staph. aureus were the commonest organisms isolated. we concluded that rheumatic and congenital heart disease are equally important in predisposition to infective endocarditis.	2008	2519331
[myocardial perfusion during exercise and rest in coronary patients. evaluation with a thallium 201 analog (isonitrile-technetium 99 m)].	isonitrile-technetium, a thallium 201 analog, was used for myocardial perfusion imaging in 12 patients with known coronary heart disease. 10-40 mcu of the isotope were injected at maximal effort during a bruce stress test. imaging was performed using 3 projections immediately after exercise and 24 hr later, using a gamma camera attached to a computer. myocardial necrosis was detected with a sensitivity of 89% and specificity of 63%. corresponding figures for stress induced ischemia were 89% and ...	2008	2519396
[uropathogenic serratia marcescens of hospital origin: analysis of the adhesion mechanism of 2 strains].	we isolated 2 serratia marcescens strains, fim (0) and fim (-), from patients with urinary tract infection. adhesion properties were studied by hemagglutination, response of uroepithelial cells and electron microscopy techniques. s marcescens fim (+) showed high adherence to uroepithelial cells, hemagglutination capacity and peculiar electron microscopy characteristics. all of these are absent in the fim (-) variety. the fim (+) strain was resistant to nitrofurantoin, nalidixic acid, tetracyclin ...	1989	2519411
the biology of the scid mutation.		2008	2519515
pimper pimped.		2008	2519529
a highly sensitive eia utilizing covalent conjugate of a polymerized enzyme and an antibody.		2008	2519546
[possibilities for purification of refuse seepage water by sand containing humic substances].	sands containing humic substances are proposed for protection of ground water from refuse tip seepage waters which containing heavy metals and organic polluting agents. such sands are produced and spoiled southern of leipzig during uncovering of brown coal. humic acids have qualities such as ion-exchange materials, form hydrogen bondes and are able to form salts and complexes. besides they can adsorb and include other substances. this properties qualify the humic acids in contact with the graine ...	2008	2525848
bidimensional reversed-phase high-pressure liquid chromatography analysis of cultured cell neuropeptides: application to atrial natriuretic factor.	we report here a one-step procedure for extraction and analysis of neuropeptides in chromaffin cell culture media and acid extracts using reversed-phase hplc. the bidimensional hplc system consists of a precolumn connected to a six-port switching valve which is on-line with an analytical column. the direct injection of the biological samples onto the precolumn previously equilibrated with 15% acetonitrile allows the elimination of interfering substances. the samples purified on the precolumn can ...	1989	2527010
determination of the secondary structure of the dna binding protein ner from phage mu using 1h homonuclear and 15n-1h heteronuclear nmr spectroscopy.	the sequential resonance assignment of the 1h and 15n nmr spectra of the dna binding protein ner from phage mu is presented. this is carried out by using a combination of 1h-1h and 1h-15n two-dimensional experiments. the availability of completely labeled 15n protein enabled us to record a variety of relayed heteronuclear multiple quantum coherence experiments, thereby enabling the correlation of proton-proton through-space and through-bond connectivities with the chemical shift of the directly ...	1989	2527559
hepatitis b vaccination in ramathibodi health personnel 1986-1987.	hevac-b-pasteur 5 mcg, h-b-vax i 20 mcg, and engerix b 20 mcg, were given to 23, 34, 15 health personnel in ramathibodi hospital. the seroconversion rate for pasteur vaccine was 85 per cent compared to 95 per cent for the other two groups. adverse reactions were few and transient.	2008	2527940
immunogenicity of a yeast-derived hepatitis b vaccine in hemodialysis patients.	in a multicenter study of hemodialysis patients in spain, the immunogenicity of a yeast-derived recombinant deoxyribonucleic acid hepatitis b vaccine was evaluated. two different vaccination schedules were examined: zero, one, two, six months and zero, one, two, 12 months. two different dose levels (20 micrograms and 40 micrograms) were also compared. no serious adverse effects were reported by any of the vaccinees; the most frequently reported reaction was soreness at the injection site. this s ...	1989	2528295
1-(3-trifluoromethylphenyl) piperazine (tfmpp) in the ventral tegmental area reduces the effect of desipramine in the forced swimming test in rats: possible role of serotonin receptors.	1-(3-trifluoromethylphenyl)piperazine (tfmpp), a serotonin1 (5-ht1) receptor agonist, injected i.p. in doses of 0.1 and 0.6 mg/kg, did not modify the immobility time of rats in the forced swimming test but significantly antagonized the effect of a 7 days treatment with 10 mg/kg per day desipramine (dmi). a similar effect was found on infusing 1 and 5 micrograms/microliters tfmpp bilaterally into the ventral tegmental area (vta). infusion of 5 micrograms/microliters tfmpp into the nucleus accumbe ...	1989	2533079
[disseminated peritoneal leiomyomatosis. a case report].	leiomyomatosis peritonealis disseminata (lpd) is a rare benign and mostly asymptomatic disease only occurring in women of reproductive age. because of the disseminated appearance of multiple subperitoneal nodules malignancy is frequently simulated. a report is given on a case of lpd in a 38 year old woman. today the cause of lpd is seen in a specific hormonal stimulation of the pluripotent subperitoneal mesenchyma of the müllerian duct in the minor pelvis. an individual disposition is discussed. ...	2008	2533772
a cell adhesion molecule, icam-1, is the major surface receptor for rhinoviruses.	rhinoviruses, which cause common colds, possess over 100 serotypes, 90% of which (the major group) share a single receptor. lymphocyte function associated molecule 1 (lfa-1) mediates leukocyte adhesion to a wide variety of cell types by binding to intercellular adhesion molecule 1 (icam-1). we demonstrate identity between the receptor for the major group of rhinoviruses and icam-1. a major group rhinovirus binds specifically to purified icam-1 and to icam-1 expressed on transfected cos cells, an ...	1957	2538244
identification of icsa, a plasmid locus of shigella flexneri that governs bacterial intra- and intercellular spread through interaction with f-actin.	the capacity of shigella to spread within the cytosol of infected epithelial cells and to infect adjacent cells is critical for the development of infection foci, which lead to mucosal abscesses. shigella is a nonmotile microorganism that appears to utilize host cell microfilaments to generate intra- as well as intercellular movements, since this movement was inhibited by cytochalasin d and involvement of f-actin was demonstrated by direct labeling of infected cells with the specific dye n-(7-ni ...	1989	2542950
human endothelial cells inhibit platelet aggregation by separately stimulating platelet cyclic amp and cyclic gmp.	thrombin-induced platelet aggregation was monitored in a mixture of washed human platelets and cultured human endothelial cells on microcarrier beads. endothelial cells completely inhibited platelet aggregation and enhanced the content of both cyclic amp and cyclic gmp in the platelets. inhibition of endothelial prostacyclin synthesis with indomethacin abolished the cyclic amp increase in the platelets, whereas the cyclic gmp increase was unimpaired. a significant component of the endothelial an ...	1989	2546782
characterization of cdna clones encoding two putative isoforms of the alpha 1 subunit of the dihydropyridine-sensitive voltage-dependent calcium channel isolated from rat brain and rat aorta.	cdna clones encoding rat brain and rat aorta isoforms of the alpha 1 subunit of the dihydropyridine-sensitive, voltage-dependent calcium channel were isolated and sequenced. these tissue-specific cdna clones share significant amino acid similarity with the rabbit skeletal muscle calcium channel alpha 1 subunit (75% and 66% amino acid identity for rat brain and rat aorta isoforms, respectively). northern analysis revealed transcript sizes of 6.5 and 8.6 kb in aorta and 8.6 kb in brain.	1989	2546805
influence of non-esterified fatty acids on respiratory control of reconstituted cytochrome-c oxidase.	bovine heart cytochrome-c oxidase was reconstituted in liposomes (asolectin) and the activity measured in the presence and absence of uncoupler at increasing concentrations of non-esterified fatty acids. palmitic and stearic acids resulted in a decrease of about 40% in the respiratory control ratio at a concentration of 1 microm, when measured using a spectrophotometric procedure but not with a polarographic assay method. at higher fatty acid concentrations no further change was found. a 50% dec ...	1989	2546826
isolation and characterisation of calcineurin from adrenal cell cytoskeleton: identification of substrates for ca2+-calmodulin-dependent phosphatase activity.	ca2+-calmodulin-dependent protein phosphatase activity is found in cytoskeletons of y-1 mouse adrenal and bovine fasciculata cells. the activity is inhibited by three inhibitors of calmodulin (trifluoperazine, w-7 and pimozide) with ec50 in the low micromolar range. protein phosphatase activity is inhibited by vanadate, fluoride, zn2+ and pyrophosphate, stimulated by mn2+ and found to be tightly bound to the cytoskeleton. substrates for endogenous phosphatase activity were defined by one- and tw ...	1989	2546840
hypoglycaemia induced by antibodies to insulin receptor following a bone marrow transplantation in an immunodeficient child.	severe hypoglycaemia developed seven months after a bone marrow transplantation in a child with severe combined immunodeficiency. his serum exerted potent insulin-like activity: (a) it stimulated insulin receptor autophosphorylation and kinase activity in cell-free systems, this effect being additive to insulin; (b) it increased glucose transport in isolated soleus muscle. these insulin-like effects were due to immunoglobulins against the insulin receptor. indeed, the patient serum immunoprecipi ...	1989	2546845
human papillomavirus and cervical cancer. summary report.		1989	2546883
[clinical and biological considerations on adenocystic carcinoma of the head].	the purpose of the present work was to define some aspects (i.e. the influence of histological grading on prognosis and the possibility of an eventual hormone dependency) of adenoidocystic carcinoma, a highly controversial form of carcinoma still open to debate. a study is thus reported of 33 cases of adenoidocystic carcinoma of the head and neck seen from 1965-1985 at the e.n.t. clinic of ferrara. the individual histological samples were seen over a period of time by a single histopathologist w ...	2008	2548367
functional organization and nucleotide sequence of virulence region-2 on the large virulence plasmid in shigella flexneri 2a.	the 7 kb virulence region-2 of the large (virulence) plasmid in shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. insertion and deletion mutagenesis, dna subcloning and sds-polyacrylamide gel electrophoresis of proteins synthesized in minicells demonstrated five genes in this region. they encode 24, 18, 62 (ipab), 41 (ipac) and 37 (ipad)-kilodalton (kd) proteins. complementation of tn5-induced mutations in region-2 with the above plasmid construc ...	1989	2552264
[computed tomographic volumetry of the orbit in endocrine orbitopathy].	the volumes of the four recti muscles and the orbital fat was measured by ct in 40 normal persons and in 60 patients with clinically confirmed graves' disease. compared with normal persons, 42 patients (70%) showed an increase in muscle volume and 28 patients (46.7%) an increase in the amount of fat. in nine patients (15%) muscle volume was normal, but the fat was increased. by using volumetric measurements, the amount of fat in the orbits in patients with graves' disease could be determined.	1968	2554416
thyrotropic activity of the ovine corticotropin-releasing factor in the chick embryo.	an injection of several doses of ovine corticotropin-releasing factor (ocrf) was made into an allantoic blood vessel of 18-day-old chick embryos. all doses used (0.5, 1, 2, and 5 micrograms) induce a quick increase of plasma corticosterone concentrations after 15 and 30 min and 1 and 2 hr and this in a dose-dependent manner with the high doses having a prolonged effect lasting up to 2 hr. an increase in plasma levels of thyroxine (t4) and triiodothyronine (t3) was observed after 1 and 2 hr. afte ...	1989	2555247
autotransplantation of avian parathyroid glands: an animal model for studying parathyroid function.	the parathyroid glands of chickens were autotransplanted and the return of parathyroid function following transplantation was determined. parathyroidectomy (ptx) resulted in a marked hypocalcemia (5.2 +/- 0.2 mg/dl) 4 hr following ptx. plasma calcium (pca) had declined to 4.3 +/- 0.2 mg/dl 24 hr after ptx. parathyroid glands were transplanted subcutaneously 24 hr after removal and 24 hr later, pca had risen to 8.6 +/- 0.5 mg/dl. seven days after ptx. pca increased to 10.3 +/- 0.2 mg/dl and by 14 ...	1989	2555250
the p-m and the 23.5 mrf (hobo) systems of hybrid dysgenesis in drosophila melanogaster are independent of each other.	strains of drosophila melanogaster bearing the male recombination factor 23.5 mrf induce hybrid dysgenesis in a way which is highly reminiscent of the p-m system, and, most probably, causally related to the activity of the transposable element hobo. we have investigated potential interactions between the two systems of hybrid dysgenesis by studying mixed lines derived from bidirectional crosses between 23.5 mrf and p strains, and analyzed their potentials to induce or suppress the occurrence of ...	1965	2555254
[assessment of diagnostic prospects for a method of immunoenzyme analysis during mass testing of feces of patients for the purpose of detecting rotavirus antigens].		1989	2555970
enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus.	we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ...	1989	2556341
breast cancer in west indian women in trinidad.	a retrospective review of breast cancer in west indian women in trinidad is presented. breast cancer constituted 17% of all cancers diagnosed and represented 26% of all cancers in females. the usual presenting symptom was a painless lump. the right breast and the upper outer quadrant were the common site of cancer. 85% of the patients were multiparous with an average of 4 children and 92% of them breast fed. most patients presented in stage i disease, but a significant number of cases were in an ...	2008	2556824
in vivo presynaptic modulation of serotonergic neurotransmission in the rat hippocampus by diazepam.	the effect of the benzodiazepine agonist, diazepam, on serotonergic (5-ht) neurotransmission was assessed in in vivo electrophysiological experiments. diazepam enhanced, in a dose-dependent manner (0.05-2 mg/kg i.v.), the effect of electrical stimulation of the ascending 5-ht pathway on the firing activity of dorsal hippocampus pyramidal neurons. this effect was blocked by the benzodiazepine antagonist, flumazenil. diazepam did not modify the efficacy of microiontophoretic application of 5-ht an ...	1989	2559857
[acute maternal anterior poliomyelitis in a non-endemic zone].	the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus.	1989	2561040
[some new points of view of cavity form].	the discovery of new data about the anatomy and physiology of the dental tissues, the improvement of the diagnostics of dental caries and the development of stomatological materials and technique are preconditions for a change of some concepts for the preparation of the cavities and their form. a new trend in cavity preparation is discussed on the base of literature data, characterized by maximum sparing of the hard dental tissues and a change in some of the geometric characteristics of the cavi ...	2008	2562576
characterization of l-glutamate action on the release of endogenous dopamine from the rat caudate-putamen.	in the present study the effect of l-glutamic acid (l-glu), n-methyl-d-aspartic acid (nmda), kainic acid (ka) and quisqualic acid (quis) on the release of endogenous dopamine (da) from slices of the rat caudate-putamen was investigated. da was measured by high-performance liquid chromatography coupled to an electrochemical detector. l-glu, nmda, ka and quis, in the absence of mg++, produced a dose-related, ca++-dependent increase in da release. the order of agonist efficacy was l-glu greater tha ...	1989	2563769
effect of endogenous opioid peptides on trh release from rat stomach in vitro.	the effect of endogenous opioid peptides (beta-endorphin, leucine-enkephalin, dynorphin 1-13 on the release of thyrotropin releasing hormone (trh) from the rat stomach in vitro was studied. the rat stomach was incubated in the medium 199 with 1.0 mg/ml of bacitracin (ph 7.4) (medium) and the amount of trh released into the medium was measured by radioimmunoassay. the release of trh from the rat stomach was inhibited significantly in a dose-related manner with the addition of endogenous opioid pe ...	1989	2565806
interleukin-6 in the epidermis of patients with psoriasis before and during puva treatment.	biopsies from lesional and unaffected skin of 6 patients with psoriasis, taken before and during treatment with psoralen plus uva (puva) were examined immunohistologically, using partially purified polyclonal antibodies to crude supernatants of activated human blood monocytes. by absorption with recombinant derived human monokines, we were able to demonstrate that interleukin-6 (il-6) (but not il-1 alpha or il-1 beta) was located in a laminar and granular pattern in stratum corneum, and on epide ...	1989	2566221
genome type analysis of adenoviruses: isolates from one year from the hannover area.	adenoviruses (av), isolated from 138 children during the year 1981 in the hannover area, were studied by dna restriction analysis with the enzymes bamhi, bglii, bsteii, ecori, hindiii, kpni, and smai and compared with the respective prototypes. varying fragment patterns were depicted and genome types analyzed. prototype-like strains of av1 and 5 were not found. types 2, 5, 1, 3, and 7 showed decreasing genetic variation in that order. altered restriction sites were physically mapped on the genom ...	1989	2566302
agonistic effects of anti-cd2 and anti-cd16 antibodies on human natural killer killing.	two monoclonal antibodies (moab), 9-1 (anti-cd2) and 3g8 (anti-cd16), were previously shown to enhance the cytotoxic activity of human natural killer (nk) cells. the present study examined the effect of 9-1 and 3g8 with different effector and target cells to determine whether they activate nk cells through a common mechanism. analysis of purified lymphocyte subpopulations demonstrated that the cd3+cd16+cd3- nk effector cell population is enhanced by both antibodies, while purified cd2+cd16-cd3+ ...	1989	2567053
long-range structure of h-ras 1-selected transgenomes.	we have used chromosome-mediated gene transfer (cmgt) and whole cell fusion to derive human-mouse hybrid cells carrying reduced human chromosomes 11, by selecting for expression of the transforming h-ras 1 oncogene. to realize the full potential of these somatic cell genetic techniques as resources for enriched dna probe isolation and the fine structure mapping of chromosomes, the nature of any molecular rearrangements that may accompany the process of dna transfer must be understood. we have an ...	1989	2567060
the cd2 antigen associates with the t-cell antigen receptor cd3 antigen complex on the surface of human t lymphocytes.	t lymphocytes can be activated by various stimuli directed either against the t-cell antigen receptor-cd3 antigen complex (ti-cd3) or the cd2 molecule; see ref. 1 for a review. activation signals generated by antigen binding to the antigen-specific alpha/beta heterodimer (ti) are thought to be transduced via the invariant cd3 gamma, epsilon and delta chains, and the associated zeta and eta subunits. the physiological role of the interaction of cd2 with its homologous cell-surface associated liga ...	1989	2567497
randomised study of myringotomy, amoxycillin/clavulanate, or both for acute otitis media in infants.	in a prospective study, 105 infants aged 3-12 months with acute otitis media were randomly assigned to one of three treatment groups: amoxycillin/clavulanate ('augmentin') alone (36 patients), myringotomy plus placebo (35 patients), or augmentin plus myringotomy (34 patients). the last two groups were double-blinded. bacterial pathogens, mainly haemophilus influenzae (of which 20% were beta-lactamase producers) and streptococcus pneumoniae, were isolated from 60% of the ear exudates and all isol ...	1989	2567903
assessment of pancreatic islet-cell population in the hyperglycemic athymic nude mouse: immunohistochemical, ultrastructural, and hormonal studies.	previous studies in diabetic animal models have demonstrated altered pancreatic islet-cell populations. to further characterize the diabetic syndrome in our athymic nude mouse colony, we studied the population of endocrine cells in pancreatic islets of 4-week-old normoglycemic and 8-week-old hyperglycemic athymic nude (nu/nu) mice using immunohistochemistry, morphometry, and electron microscopy. in normoglycemic 4-week athymic nu/nu mice, the proportions of b (insulin-secreting) cells and a (glu ...	1989	2569196
priapism: current concepts.	the treatment options for the management of the patient with priapism have changed markedly within the past several years. when possible, the underlying cause of the priapism should be identified. therapy should be guided by the results of aspiration of the blood-filled corpora cavernosa of the erect penis. early intervention through pharmacologic manipulation or surgical shunting should not be delayed while trying conservative measures.	2001	2569852
priapism: current concepts.	the treatment options for the management of the patient with priapism have changed markedly within the past several years. when possible, the underlying cause of the priapism should be identified. therapy should be guided by the results of aspiration of the blood-filled corpora cavernosa of the erect penis. early intervention through pharmacologic manipulation or surgical shunting should not be delayed while trying conservative measures.	2001	2569852