Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| structure of a new azurin from the denitrifying bacterium alcaligenes xylosoxidans at high resolution. | it has been reported previously that alcaligenes xylosoxidans (nc1mb 11015) grown under denitrifying conditions produces two azurins instead of the single previously identified azurin [dodd, hasnain, hunter, abraham, debenham, kanzler, eldridge, eady, ambler & smith (1995). biochemistry. in the press]. the new azurin, called azurin ii, has been crystallized as blue elongated rectangular prisms with the tetragonal space group p4(1)22 and unit-cell parameters a = b = 52.65, c = 100.63 a. x-ray cry ... | 1995 | 15299774 |
| primary sequence and refined tertiary structure of pseudomonas fluorescens holo azurin at 2.05 a. | this paper reports the primary sequence and refined crystal structure of pseudomonas fluorescens holoazurin. the crystal structure has been determined by molecular replacement on the basis of the molecular model of azurin from alcaligenes denitrificans, and refined by the method of molecular dynamics simulation and energy-restrained least-squares methods. p. fluorescens was crystallized in the orthorhombic space group p2(1)2(1)2(1) with unit-cell dimensions a = 31.95, b = 43.78, c = 78.81 a. the ... | 1997 | 15299879 |
| structures of a blue-copper nitrite reductase and its substrate-bound complex. | copper-containing nitrite reductases (nir's) have been conveniently subdivided into blue and green nir's which are thought to be redox partners of azurins and pseudo-azurins, respectively. crystal structures of two green nir's have recently been determined. alcaligenes xylosoxidans has been shown to have a blue-copper nitrite reductase (axnir) and two azurins with 67% homology both of which donate electrons to it effectively. the first crystal structure of a blue nir (axnir) in its oxidized and ... | 1997 | 15299906 |
| crystallization and preliminary x-ray studies of azurin-i and azurin-ii from denitrifying bacterium alcaligenes xylosoxidans gifu 1051. | two distinct azurins named azurin-i and azurin-ii were isolated from alcaligenes xylosoxidans gifu 1051 (azg-i and azg-ii). they have been purified and crystallized. the crystals of azg-i belong to the monoclinic crystal system, space group c2, unit-cell parameters a = 130.6, b = 54.4, c = 74.7 a, beta = 96.1 degrees. the crystals of azg-ii belong to the tetragonal crystal system, space group p4(1)22 or p4(3)22, unit-cell parameters a = b = 52.6, c = 100.7 a. both crystals diffract up to 2.0 a r ... | 1997 | 15299941 |
| bidirectional catalysis by copper-containing nitrite reductase. | the copper-containing nitrite reductase from alcaligenes faecalis s-6 was found to catalyze the oxidation of nitric oxide to nitrite, the reverse of its physiological reaction. thermodynamic and kinetic constants with the physiological electron donor pseudoazurin were determined for both directions of the catalyzed reaction in the ph range of 6-8. for this, nitric oxide was monitored by a clark-type electrode, and the redox state of pseudoazurin was measured by optical spectroscopy. the equilibr ... | 2004 | 15301545 |
| conversion of industrial food wastes by alcaligenes latus into polyhydroxyalkanoates. | broader usage of biodegradable plastics in packaging and disposable products as a solution to environmental problems would heavily depend on further reduction of costs and the discovery of novel biodegradable plastics with improved properties. as the first step in our pursuit of eventual usage of industrial food wastewater as nutrients for microorganisms to synthesise environmental-friendly bioplastics, we investigated the usage of soya wastes from a soya milk dairy, and malt wastes from a beer ... | 1999 | 15304714 |
| treatment of dairy wastewater using a selected bacterial isolate, alcaligenes sp. mmrr7. | physicochemical and biologic analysis of dairy wastewater showed that the effluent had a high organic load (chemical oxygen demand [cod]: 5095 mg/l), an acidic ph (6.4), and a high probability of coliforms (most probable number [mpn] >1100). the various bacterial strains isolated and purified were identified as sporolactobacillus sp., citrobacter sp., pseudomonas sp., alcaligenes sp., bacillus sp., staphylococcus sp., and proteus sp., as per the bergey's manual of systematic bacteriology. among ... | 2004 | 15304739 |
| [microbial degradation of mustard gas reaction masses: isolation and selection of degradative microorganisms, analysis of organic components of reaction masses and their biodegradation]. | bacterial strains growing in medium with mustard gas reaction masses (rm) as carbon sources were obtained. growth cessation in the above medium was caused by the exhaustion of bioutilizable substrates, first of all monoethanolamine (mea) and ethyleneglycol (eg), rather than by the accumulation of toxic metabolites in the culture liquid or in the cells. the main rm components, 1,4-perhydrothiazines (pht), formed in the course of chemical detoxication of mustard gas, were identified and analyzed. ... | 2004 | 15315229 |
| biological control of fusarium wilt of pigeonpea cajanus cajan (l.) millsp with chitinolytic alcaligenes xylosoxydans. | alcaligenes xylosoxydans protected pigeonpea from fusarium wilt in a pot experiment and field trials. when seeds of pigeonpea (c. cajan) were treated with a. xylosoxydans and sown in soil infested with fusarium, the incidence of wilt was reduced by 43.5% and resulted in 58% higher grain yield. the antifungal activity of a. xylosoxydans was based on chitinase production and was comparable in efficacy to commercial antifungal agents such as benlate, monitor wp, thiram and bavistin. | 2003 | 15320506 |
| microdiversity of phenol hydroxylase genes among phenol-degrading isolates of alcaligenes sp. from an activated sludge system. | enterobacterial repetitive intergenic consensus (eric)-pcr fingerprinting classified 97 phenol-degrading isolates with identical amplified ribosomal dna restriction analysis (ardra) patterns into six genotypic groups. the 16s rrna gene of the representative isolate of each group had higher than 99.47% common identity with each other and higher than 98% identity with the type strain of alcaligenes faecalis. pcr-tgge (temperature gradient gel electrophoresis) analysis of the genes of the largest s ... | 2004 | 15321685 |
| [the degradation characteristics of degrading bacterium of 2,6-di-tert-butylphenol]. | a degrading bacterial strain f-3-4 for 2,6-di-tert-butylphenol (2,6-dtbp) was isolated from biofilm in acrylic fiber wastewater treatment structures. by acclimation, its capacity for degradation of 2,6-dtbp was enhanced by 26%. it was identified as alcaligenes sp. according to morphological, physiological and biochemical characters. by tests in shaking flasks, the effects of the conditions of growth was studied, and it was determined that the optimum conditions of growth for the strain was 37 de ... | 2004 | 15327263 |
| bacterial communities and enzyme activities of pahs polluted soils. | three soils (i.e. a belgian soil, b-bt, a german soil, g, and an italian agricultural soil, i-bt) with different properties and hydrocarbon-pollution history with regard to their potential to degrade phenanthrene were investigated. a chemical and microbiological evaluation of soils was done using measurements of routine chemical properties, bacterial counts and several enzyme activities. the three soils showed different levels of polycyclic aromatic hydrocarbons (pahs), being their contamination ... | 2004 | 15331267 |
| biofuel cells select for microbial consortia that self-mediate electron transfer. | microbial fuel cells hold great promise as a sustainable biotechnological solution to future energy needs. current efforts to improve the efficiency of such fuel cells are limited by the lack of knowledge about the microbial ecology of these systems. the purposes of this study were (i) to elucidate whether a bacterial community, either suspended or attached to an electrode, can evolve in a microbial fuel cell to bring about higher power output, and (ii) to identify species responsible for the el ... | 2004 | 15345423 |
| purification and characterization of aromatic amine dehydrogenase from alcaligenes xylosoxidans. | aromatic amine dehydrogenase was purified and characterized from alcaligenes xylosoxidans ifo13495 grown on beta-phenylethylamine. the molecular mass of the enzyme was 95.5 kda. the enzyme consisted of heterotetrameric subunits (alpha2beta2) with two different molecular masses of 42.3 kda and 15.2 kda. the n-terminal amino acid sequences of the alpha-subunit (42.3-kda subunit) and the beta-subunit (15.2-kda subunit) were dlpieelxggtrlpp and apaagnkxpqmddta respectively. the enzyme had a quinone ... | 2004 | 15388968 |
| bacteremia caused by achromobacter and alcaligenes species in 46 patients with cancer (1989-2003). | achromobacter and alcaligenes are emerging infectious gram-negative bacterial species that can affect immunosuppressed patients. the authors sought to determine the incidence and characteristics of bloodstream infections caused by these organisms in patients with underlying malignancies. | 2004 | 15389476 |
| antibiograms of resistant gram-negative bacteria from scottish cf patients. | over a 19-month pilot phase, 93 multiply resistant gram-negative isolates from scottish cystic fibrosis patients were sent to a referral laboratory for further investigation. | 2004 | 15463901 |
| a survey of the composition and diversity of bacterial populations in bleached kraft pulp-mill wastewater secondary treatment systems. | bacterial community compositions from 10 pulp- and paper-mill treatment systems were compared using both traditional and molecular techniques. 16s-rflp (random fragment length polymorphisms) analysis was used to examine the genotypic profiles of the whole bacterial community of each treatment system. although all the communities shared approximately 60% of their dna band pattern, as determined by computer-assisted cluster analysis, each community displayed a unique profile that was stable over t ... | 2004 | 15467789 |
| structure-based engineering of alcaligenes xylosoxidans copper-containing nitrite reductase enhances intermolecular electron transfer reaction with pseudoazurin. | the intermolecular electron transfer from achromobacter cycloclastes pseudoazurin (acpaz) to wild-type and mutant alcaligenes xylosoxidans nitrite reductases (axnirs) was investigated using steady-state kinetics and electrochemical methods. the affinity and the electron transfer reaction constant (k(et)) are considerably lower between acpaz and axnir (k(m) = 1.34 mm and k(et) = 0.87 x 10(5) m(-1) s(-1)) than between acpaz and its cognate nitrite reductase (acnir) (k(m) = 20 microm and k(et) = 7. ... | 2004 | 15475344 |
| cloning and functional analysis by gene disruption of a novel gene involved in indigo production and fluoranthene metabolism in pseudomonas alcaligenes pa-10. | a novel indole dioxygenase (idoa) gene has been cloned from pseudomonas alcaligenes pa-10, based on its ability to convert indole to indigo. the chromosomally encoded idoa gene exhibits no similarity to previously cloned naphthalene dioxygenases or to aromatic oxygenases from other species at the nucleotide level. phylogenetic analysis indicates that the idoa gene product is most similar to an acyl-coa dehydrogenase from novosphingobium aromaticivorans. the enzyme encoded by the idoa gene is ess ... | 2004 | 15476978 |
| quantification of denitrifying bacteria in soils by nirk gene targeted real-time pcr. | denitrification, the reduction of nitrate to nitrous oxide or dinitrogen, is the major biological mechanism by which fixed nitrogen returns to the atmosphere from soil and water. microorganisms capable of denitrification are widely distributed in the environment but little is known about their abundance since quantification is performed using fastidious and time-consuming mpn-based approaches. we used real-time pcr to quantify the denitrifying nitrite reductase gene (nirk), a key enzyme of the d ... | 2004 | 15488276 |
| characterization of two type 1 cu sites of hyphomicrobium denitrificans nitrite reductase: a new class of copper-containing nitrite reductases. | we report (1) the amino acid sequence of hyphomicrobium denitrificans nitrite reductase (hdnir), containing two type 1 cu sites and one type 2 cu site; (2) the expression and preparation of wild-type hdnir and two mutants replacing the cys ligand of each type 1 cu with ala; and (3) their spectroscopic and functional characterization. the open-reading frame of 50-kda hdnir is composed of the 15-kda n-terminal domain having a type 1 cu-binding motif like cupredoxins and the 35-kda c-terminal domai ... | 2004 | 15518568 |
| in vitro antimicrobial activity of gatifloxacin compared with other quinolones against clinical isolates from cancer patients. | owing to the predominance of gram-positive pathogens in neutropenic cancer patients, newer generation quinolones with an expanded gram-positive spectrum and enhanced potency, may have a role to play for prophylaxis and/or empiric therapy in such patients. the in vitro activity of gatifloxacin was compared with that of ciprofloxacin, levofloxacin and trovafloxacin against 848 recent clinical isolates from cancer patients. against gram-positive organisms, gatifloxacin was the most active agent tes ... | 2004 | 15523180 |
| growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. | twenty-seven honey samples from different floral sources and geographical locations were evaluated for their ability to inhibit the growth of seven food spoilage organisms (alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, geotrichum candidum, lactobacillus acidophilus, penicillium expansum, pseudomonas fluorescens) and five foodborne pathogens (bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica ser. typhimurium, and staphylococcus aureus) ... | 2004 | 15527912 |
| bacterium-based no2- biosensor for environmental applications. | a sensitive no2- biosensor that is based on bacterial reduction of no2- to n2o and subsequent detection of the n2o by a built-in electrochemical n2o sensor was developed. four different denitrifying organisms lacking no3- reductase activity were assessed for use in the biosensor. the relevant physiological aspects examined included denitrifying characteristics, growth rate, no2- tolerance, and temperature and salinity effects on the growth rate. two organisms were successfully used in the biosen ... | 2004 | 15528518 |
| poly(3-hydroxybutyrate) biosynthesis in the biofilm of alcaligenes eutrophus, using glucose enzymatically released from pulp fiber sludge. | glucose, enzymatically released from pulp fiber sludge, was combined with inorganic salts and used as a growth medium for alcaligenes eutrophus, a gram-negative strain producing poly(3-hydroxybutyrate) (phb). by controlling the concentrations of the inorganic salts in the growth medium, almost 78% of the cell mass was converted to pure phb. efforts were made to find conditions for bacterial growth in the form of a biofilm on a cheap and reusable carrier. a number of positively charged carriers w ... | 2004 | 15528544 |
| [a new n-acyl derivative of (s)-cysteine for quantitative determination of enantiomers of amino compounds by hplc method with a precolumn modification with o-phthalaldehyde]. | n-phenylacetyl-(r)-phenylglycyl-(s)-cysteine (nppc) was used for the determination of enantiomers of primary amines by rphplc with a precolumn modification with o-phthalaldehyde. nppc was compared with the classic sh reagent n-acetyl-(s)-cysteine (nac) in the analysis of stereomers of nonfunctionalized amines and amino alcohols. after the nac modification, the resulting diastereomeric isoindoles were difficult to separate by hplc, and satisfactory resolution was achieved only for some aliphatic ... | 2004 | 15562964 |
| comparison of aerobic denitrifying activity among three cultural species with various carbon sources. | abilities of three aerobic denitrifiers such as alcaligenes faecalis, microvirgula aerodenitrificans and paracoccus pantotrophus were compared from the viewpoints of nitrate removal efficiency and organic matter utilization. first, the effect of carbon source was investigated. although nitrate reduction was observed in all strains under aerobic conditions, a change of carbon source considerably affected the denitrification ability. in the case of p. pantotrophus, nitrate and nitrite were complet ... | 2004 | 15566182 |
| in-situ characterization of microbial community in an a/o submerged membrane bioreactor with nitrogen removal. | the bacterial community involved in removing nitrogen from sewage and their preferred do environment within an anoxic/oxic membrane bioreactor (a/o mbr) was investigated. a continuously operated laboratory-scale a/o mbr was maintained for 360 d. at a sludge age of 150 d and a c/n ratio of 3.5, the system was capable of removing 88% of the influent nitrogen from raw wastewater through typical nitrogen removal transformations (i.e. aerobic ammonia oxidation and anoxic nitrate reduction). character ... | 2004 | 15566185 |
| site-directed mutagenesis alters dnak-dependent folding process. | the overproduction of d-aminoacylase (a6-d-anase) of alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) is accompanied by aggregation of the overproduced protein, and its soluble expression is facilitated by the coexpression of dnak-dnaj-grpe (dnakje). when the a6-d-anase gene was expressed in the escherichia coli dnak mutant dnak756, little activity was observed in the soluble fraction, and it was restored by the coexpression of dnakje or the substitution of the r354 residue of ... | 2005 | 15567154 |
| insights into redox partner interactions and substrate binding in nitrite reductase from alcaligenes xylosoxidans: crystal structures of the trp138his and his313gln mutants. | dissimilatory nitrite reductase catalyses the reduction of nitrite to nitric oxide within the key biological process of denitrification. we present biochemical and structural results on two key mutants, one postulated to be important for the interaction with the partner protein and the other for substrate entry. trp138, adjacent to one of the type-1 cu ligands, is one of the residues surrounding a small depression speculated to be important in complex formation with the physiological redox partn ... | 2004 | 15610025 |
| hydrolysis of nitriles using an immobilized nitrilase: applications to the synthesis of methionine hydroxy analogue derivatives. | mild and selective hydrolysis of a large range of nitriles leading to carboxylic acids was achieved under neutral conditions by an immobilized and genetically modified enzyme preparation from alcaligenes faecalis atcc8750. this immobilized nitrilase has been shown to be an effective catalyst for the stereoselective hydrolysis of mandelonitrile 1a to r-(-)-mandelic acid 1c. this method is particularly useful for the production of hydroxy analogues of methionine derivatives 2c-4c that could have a ... | 2004 | 15612811 |
| heterologous metalloprotein biosynthesis in escherichia coli: conditions for the overproduction of functional copper-containing nitrite reductase and azurin from alcaligenes xylosoxidans. | this paper reports on the optimization of conditions for the overproduction and isolation of two recombinant copper metalloproteins, originally encoded on the chromosome of the dentrifying soil bacterium alcaligenes xylosoxidans, in the heterologous host escherichia coli. the trimeric enzyme nitrite reductase (nir) contains both type-1 and type-2 cu centres, whilst its putative redox partner, azurin i, is monomeric and has only a type-1 cu centre. both proteins were processed and exported to the ... | 2004 | 15616359 |
| bacteriospermia in extended porcine semen. | bacteriospermia is a frequent finding in freshly extended porcine semen and can result in detrimental effects on semen quality and longevity if left uncontrolled. the primary source of bacterial contamination is the boar. other sources that have been identified include environment, personnel, and the water used for extender preparation. a 1-year retrospective study was performed on submissions of extended porcine semen for routine quality control bacteriological screening at the university of pe ... | 2005 | 15626417 |
| genetic diversity and horizontal transfer of genes involved in oxidation of reduced phosphorus compounds by alcaligenes faecalis wm2072. | enrichment was performed to isolate organisms that could utilize reduced phosphorus compounds as their sole phosphorus sources. one isolate that grew well with either hypophosphite or phosphite was identified by 16s rrna gene analysis as a strain of alcaligenes faecalis. the genes required for oxidation of hypophosphite and phosphite by this organism were identified by using transposon mutagenesis and include homologs of the ptxd and htxa genes of pseudomonas stutzeri wm88, which encode an nad-d ... | 2005 | 15640200 |
| enzymatic and non-enzymatic degradation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters produced by alcaligenes sp. mt-16. | poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3hb-co-3hv), copolyesters with a variety of 3hv contents (ranging from 17 to 60 mol%) were produced by alcaligenes sp. mt-16 grown on a medium containing glucose and levulinic acid in various ratios, and the effects of hydrophilicity and crystallinity on the degradability of the copolyesters were evaluated. measurements of thermo-mechanical properties and fourier-transform infrared spectroscopy in the attenuated total reflectance revealed that t ... | 2004 | 15650693 |
| advenella incenata gen. nov., sp. nov., a novel member of the alcaligenaceae, isolated from various clinical samples. | a polyphasic taxonomic study of 14 isolates recovered from various human and veterinary clinical samples was performed. phenotypically these isolates shared several characteristics with members of the alcaligenaceae and related genera. random amplified polymorphic dna fingerprinting and whole-cell protein analysis suggested the presence of multiple genomic groups, which was confirmed by dna-dna hybridization experiments. 16s rrna gene sequence analysis indicated that these isolates were related ... | 2005 | 15653883 |
| pancreatic abscess secondary to alcaligenes faecalis. | we report a patient with pancreatic abscesses and necrosis secondary to alcaligenes faecalis infection. he initially presented with alcohol-induced acute pancreatitis. twenty days after the initial presentation, he re-presented with increasing pain and was found to have pancreatic necrosis and abscesses. treatment was initiated with meropenem. because of persistent fevers, computed tomography-guided drainage was performed. the fluid grew a faecalis resistant to meropenem and the patient continue ... | 2005 | 15654182 |
| antimicrobial susceptibility of uncommonly isolated non-enteric gram-negative bacilli. | the frequency of occurrence and antimicrobial susceptibility patterns of 3059 non-enteric gram-negative bacilli (ngb), other than pseudomonas aeruginosa and acinetobacter spp., consecutively collected as part of the sentry antimicrobial surveillance program (1997-2003) were reviewed. during this period, a total of 221,084 bacterial isolates were collected from several clinical specimens worldwide, including 25,305 (11.5%) ngb. acinetobacter spp. and p. aeruginosa accounted for 82.7% of the ngb i ... | 2005 | 15664479 |
| complete conversion of nitrate into dinitrogen gas in co-cultures of denitrifying bacteria. | in the past 10 years many molecular aspects of microbial nitrate reduction have been elucidated, but the ecophysiology of this process is hardly understood. in this contribution, our efforts to study the complex microbial communities and interactions involved in the reduction of nitrate to dinitrogen gas are summarized. the initial work concentrated on emission of the greenhouse gas nitrous oxide during incomplete denitrification by alcaligenes faecalis. as more research methods became available ... | 2005 | 15667308 |
| effect from multiple episodes of inadequate empiric antibiotic therapy for ventilator-associated pneumonia on morbidity and mortality among critically ill trauma patients. | empiric antibiotic therapy is routinely initiated for patients with presumed ventilator-associated pneumonia (vap). the impact of inadequate empiric antibiotic therapy (ieat) may vary among critically ill populations. the purpose of this retrospective study was to determine the effect of ieat on the outcome for adult trauma patients with vap. | 2005 | 15674157 |
| optimization of technological conditions for one-pot synthesis of (s)-alpha-cyano-3-phenoxybenzyl acetate in organic media. | optically active form of alpha-cyano-3-phenoxybenzyl (cpb) alcohol, building block of pyrethroid insecticides, was synthesized as its acetate by the combination of anion-exchange resin (d301)-catalyzed transcyanation between m-phenoxybenzaldehyde (m-pba) and acetone cyanohydrin (ac), and lipase (from alcaligenes sp.)-catalyzed enantioselective transesterification of the resulting cyanohydrin with vinyl acetate. through optimizing technological conditions, the catalyzing efficiency was improved c ... | 2005 | 15682501 |
| genes and enzymes involved in bacterial oxidation and reduction of inorganic arsenic. | 2005 | 15691908 | |
| biodegradation of fenoxaprop-p-ethyl by bacteria isolated from sludge. | a mixed bacterial population was isolated using enrichment in a basal medium containing increasing amounts of fenoxaprop-p-ethyl as a sole carbon source from sludge that had been exposed to fenoxaprop-p-ethyl production wastewater for about 2 years. eight kinds of isolates could utilize fenoxaprop-p-ethyl, but only one was identified belonging to genus alcaligenes, named alcaligenes sp. h. in pure culture, there was 45.8, 66.0 and 69.5% loss of fenoxaprop-p-ethyl (initial concentration: 100, 50, ... | 2005 | 15721550 |
| establishment of a genetically marked insect-derived symbiont in multiple host plants. | alcaligenes xylosoxidans subsp. denitrificans, originally isolated from the cibarial region of the foregut of the glassy-winged sharpshooter (homalodisca coagulata), was transformed using the himar1 transposition system to express egfp. seedlings of six potential host plants were inoculated with transformed bacteria and 2 weeks later samples were taken 5 cm away and analyzed by quantitative real-time pcr using primers designed to amplify the gene insert. the largest colony of 3,591,427 cells/2 c ... | 2005 | 15723145 |
| bacterial community dynamics during in-situ bioremediation of petroleum waste sludge in landfarming sites. | in-situ bioremediation of petroleum waste sludge in landfarming sites of motor oil hellas (petroleum refinery) was studied by monitoring the changes of the petroleum composition of the waste sludge, as well as the changes in the structure of the microbial community, for a time period of 14 months. the analyses indicated an enhanced degradation of the petroleum hydrocarbons in the landfarming areas. a depletion of n-alkanes of approximately 75-100% was obtained. marked changes of the microbial co ... | 2005 | 15730027 |
| prospective evaluation of emerging bacteria in cystic fibrosis. | bacteria contribute considerably to the progression of lung disease in cystic fibrosis. in this prospective, multi-centre study, we aimed to evaluate the occurrence of emerging bacteria and the physicians' assessments of the clinical importance of these findings. | 2005 | 15752680 |
| changing bacterial isolates and antibiotic sensitivities of purulent dacryocystitis. | to examine the current spectrum of bacterial isolates in cases of chronic dacryocystitis and dacryoabcess and to determine their antibiotic sensitivities. | 2005 | 15764113 |
| role of arginine residues of d-aminoacylase from alcaligenes xylosoxydans subsp. xylosoxydans a-6. | to investigate the role of arginine in the folding of d-aminoacylase, seven arginine residues, r26, r152, r296, r302, r354, r377, and r391, among twelve arginine residues highly conserved in d-aminoacylase, n-acyl-d-aspartate amidohydrolase (d-aaase), and n-acyl-d-glutamate amidohydrolase (d-agase) from alcaligenes xylosoxydans subsp. xylosoxydans a-6 (alcaligenes a-6) were substituted with lysine by site-directed mutagenesis. the mutants, r26k, r152k, r296k, and r302k were identified as mutatio ... | 2005 | 15777281 |
| ca2+ is a cofactor required for membrane transport and maturation and is a yield-determining factor in high cell density penicillin amidase production. | penicillin amidases (pas) from e. coli and a. faecalis are periplasmic enzymes that contain one tightly bound ca(2+) per molecule that does not directly participate in the enzymatic function. this ion may, however, be required for the maturation of the pre-pro-enzyme. the pro-enzyme of homologous pas are translocated through the tat- (e. coli pa(ec)) and sec- (a. faecalis pa(af)) transport systems, respectively. cell fractionation, electrophoresis, immunoblotting, and activity staining demonstra ... | 2005 | 15801782 |
| [characterization of microbial population present in the edible seaweed, monostroma undulatum, wittrock]. | the microbiological quality of monostroma undulatum, wittrock from the southern argentinean coast, was studied for its application for human food. also the diversity and function of the native bacterial population to this green seaweed was analyzed. samples were collected in puerto deseado, province of santa cruz, southern argentina (47 degrees 45'l.s., 65 degrees 55'l.w). the samples were analyzed for the presence of psycotrophic heterotrophic bacteria, marine heterotrophic bacteria, low nutrit ... | 2004 | 15807211 |
| proteome investigation of the global regulatory role of sigma 54 in response to gentisate induction in pseudomonas alcaligenes ncimb 9867. | pseudomonas alcaligenes ncimb 9867 (strain p25x) utilizes the gentisate pathway for the degradation of aromatic hydrocarbons. the gene encoding the alternative sigma (sigma) factor sigma(54), rpon, was cloned from strain p25x and a rpon knock-out strain, designated g54, was constructed by insertional inactivation with a kanamycin resistance gene cassette. the role of sigma(54) in the physiological response of p. alcaligenes p25x to gentisate induction was assessed by comparing the global protein ... | 2005 | 15815998 |
| recurrent alcaligenes xylosoxidans keratitis. | to describe a case of postkeratoplasty recurrent alcaligenes xylosoxidans keratitis. | 2005 | 15829812 |
| a convergent enantioselective route to structurally diverse 6-deoxytetracycline antibiotics. | complex antibiotics based on natural products are almost invariably prepared by semisynthesis, or chemical transformation of the isolated natural products. this approach greatly limits the range of accessible structures that might be studied as new antibiotic candidates. here we report a short and enantioselective synthetic route to a diverse range of 6-deoxytetracycline antibiotics. the common feature of this class is a scaffold of four linearly fused rings, labeled a through d. we targeted not ... | 2005 | 15831754 |
| pro-sequence and ca2+-binding: implications for folding and maturation of ntn-hydrolase penicillin amidase from e. coli. | penicillin amidase (pa) is a bacterial periplasmic enzyme synthesized as a pre-pro-pa precursor. the pre-sequence mediates membrane translocation. the intramolecular pro-sequence is expressed along with the a and b chains but is rapidly removed in an autocatalytic manner. in extensive studies we show here that the pro-peptide is required for the correct folding of pa. pro-pa and pa unfold via a biphasic transition that is more pronounced in the case of pa. according to size-exclusion chromatogra ... | 2005 | 15843029 |
| imidazole antibiotics inhibit the nitric oxide dioxygenase function of microbial flavohemoglobin. | flavohemoglobins metabolize nitric oxide (no) to nitrate and protect bacteria and fungi from no-mediated damage, growth inhibition, and killing by no-releasing immune cells. antimicrobial imidazoles were tested for their ability to coordinate flavohemoglobin and inhibit its no dioxygenase (nod) function. miconazole, econazole, clotrimazole, and ketoconazole inhibited the nod activity of escherichia coli flavohemoglobin with apparent k(i) values of 80, 550, 1,300, and 5,000 nm, respectively. sacc ... | 2005 | 15855504 |
| isolation of infectious cystic fibrosis patients: results of a systematic review. | respiratory tract infections significantly contribute to morbidity and mortality among cystic fibrosis (cf) patients. therefore, pathogen transmission needs to be prevented. there are several guidelines for the care of cf patients, but no transparent systematic literature review has been published. | 2005 | 15865277 |
| pusillimonas noertemannii gen. nov., sp. nov., a new member of the family alcaligenaceae that degrades substituted salicylates. | the taxonomic position of a pseudomonas-like strain, designated bn9(t), was investigated. this strain had previously been isolated as a 5-aminosalicylate-degrading organism from a 6-aminonaphthalene-2-sulphonate-degrading mixed bacterial culture. previously, detection of ubiquinone q-8, a polyamine pattern with putrescine, spermidine and 2-hydroxyputrescine as the major polyamines, and partial 16s rrna gene sequencing had suggested that strain bn9(t) belongs to the 'betaproteobacteria'. this was ... | 2005 | 15879236 |
| sensing nitrite through a pseudoazurin-nitrite reductase electron transfer relay. | nitrite is converted to nitric oxide by haem or copper-containing enzymes in denitrifying bacteria during the process of denitrification. in designing an efficient biosensor, this enzymic turnover must be quantitatively assessed. the enzyme nitrite reductase from alcaligenes faecalis contains a redox-active blue copper centre and a nonblue enzyme-active copper centre. it can be covalently tethered to modified gold-electrode surfaces in configurations in which direct electron transfer is possible ... | 2005 | 15900523 |
| nitric oxide interaction with cytochrome c' and its relevance to guanylate cyclase. why does the iron histidine bond break? | soluble guanylate cyclase (sgc), the mammalian receptor for nitric oxide (no), is a heme protein with a histidine as the proximal ligand. formation of a five-coordinate heme-no complex with the associated fe-his bond cleavage is believed to trigger a conformational change that activates the enzyme and transduces the no signal. cytochrome c' (cyt c') is a protobacteria heme protein that has several similarities with sgc, including the ability to form a five-coordinate no adduct and the fact that ... | 2005 | 15913362 |
| hydrocarbon biodegradation in oxygen-limited sequential batch reactors by consortium from weathered, oil-contaminated soil. | we studied the use of sequential batch reactors under oxygen limitation to improve and maintain consortium ability to biodegrade hydrocarbons. air-agitated tubular reactors (2.5 l) were operated for 20 sequential 21-day cycles. maya crude oil-paraffin mixture (13,000 mg/l) was used as the sole carbon source. the reactors were inoculated with a consortium from the rhizosphere of cyperus laxus, a native plant that grows naturally in weathered, contaminated soil. oxygen limitation was induced in th ... | 2005 | 15920621 |
| role of xcpp in the functionality of the pseudomonas aeruginosa secreton. | in gram-negative bacteria, most signal-peptide-dependent exoproteins are secreted via the type ii secretion system (t2ss or secreton). in pseudomonas aeruginosa, t2ss consists of twelve xcp proteins (xcpa and xcpp to xcpz) thought to be organized as a multiproteic complex within the envelope. although well conserved, t2ss are known to be species-specific, namely for distant organisms, and this characteristic was thought to involve xcpp. to check which domain of xcpp could be involved in the spec ... | 2005 | 15936176 |
| accessibility of the distal heme face, rather than fe-his bond strength, determines the heme-nitrosyl coordination number of cytochromes c': evidence from spectroscopic studies. | the heme coordination chemistry and spectroscopic properties of rhodobacter capsulatus cytochrome c' (rccp) have been compared to data from alcaligenes xylosoxidans (axcp), with the aim of understanding the basis for their different reactivities with nitric oxide (no). whereas ferrous axcp reacts with no to form a predominantly five-coordinate heme-nitrosyl complex via a six-coordinate intermediate, rccp forms an equilibrium mixture of six-coordinate and five-coordinate heme-nitrosyl species in ... | 2005 | 15952773 |
| [constitutive expression and purification of alcaligenes faecalis penicillin g acylase in escherichia coli]. | considering alcaligenes faecalis pencillin g acylase(afpga), which possesses the attractive characteristics for beta-lactam antibiotics conversions, the gene of pga was cloned into an expressing vector pkkfpga. the recombinant plasmid contained multicopy replicon(cole 1), trc promoter, afpga gene, rrnb transcript terminator and ampicillin marker transformed escherichia coli dh5alpha. as both the recombinant plasmid and the host dh5alpha had no laclq gene, the trc promoter was always active and t ... | 2004 | 15974000 |
| alcaligenes xylosoxidans bacteremia: clinical features and microbiological characteristics of isolates. | bacteremia caused by alcaligenes xylosoxidans is rare. between 1999 and 2002, 12 cases of bacteremia caused by a. xylosoxidans were diagnosed at a tertiary referral center in central taiwan. the clinical features of these patients and the antimicrobial susceptibilities and pulsed-field gel electrophoresis (pfge) pattern of their blood isolates were studied. all infections were acquired nosocomially. all of the adult patients had underlying diseases, and 10 (83%) had undergone an invasive procedu ... | 2005 | 15986070 |
| enzymatic transformation of bacterial polyhydroxyalkanoates into repolymerizable oligomers directed towards chemical recycling. | the enzymatic transformation into an oligomer was carried out with the objective of developing the chemical recycling of bacterial polyesters. poly(r-3-hydroxyalkanoate)s (phas), such as poly[(r-3-hydroxybutyrate)-co-12%(r-3-hydroxyhexanoate)] and poly[(r-3-hydroxybutyrate)-co-12%(r-3-hydroxyvalerate)], were degraded by granulated candida antarctica lipase b immobilized on hydrophilic silica (lipase gca) in a diluted organic solvent at 70 degrees c. the degradation products were cyclic oligomers ... | 2005 | 15988790 |
| alcaligenes faecalis kw-a biofilm for denitrification of nitrate-rich effluent. | alcaligenes faecalis kw-a selected for possessing good denitrification efficiency was used for biofilm development. the biofilm could be developed on a glass surface within 12 hr when 5%, ix 10(8) cells/ml was used as inoculum. the microcolonies were seen in 6 hr and glycocalyx in 9 hr stage. at 24 hr the biofilm was developed fully and hence was visualised as dense mass. the biofilm protein content showed 48.5% increase in shake flask than in static condition. the exopoplymer is produced in lar ... | 2005 | 15991580 |
| purification of siderophores of alcaligenes faecalis on amberlite xad. | studies on siderophore production using alcaligenes faecalis bccm id 2374 revealed hydroxamate and catecholate type of siderophores at 347 microg ml-1. these fractions were purified on amberlite xad-4 column, which resulted in the separation of two bands having absorption maxima at 264 and 224 nm. the amount of pure siderophore obtained in powdered form from first and second fraction was 297 and 50 microg ml-1 respectively. | 2006 | 15993053 |
| molecular characterization of extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase genes from pseudomonas alcaligenes strains. | a bacterial strain m4-7 capable of degrading various polyesters, such as poly(epsilon-caprolactone), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3-hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate), was isolated from a marine environment and identified as pseudomonas alcaligenes. the relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (mcl-pha) depolymerase (phaz(palm4-7)) from p. alcaligenes m4-7 was 28.0 kda, as determined by sds-page. t ... | 2005 | 15995648 |
| characterization of beta-carotene ketolases, crtw, from marine bacteria by complementation analysis in escherichia coli. | a complementation analysis was performed in escherichia coli to evaluate the efficiency of beta-carotene ketolases (crtw) from the marine bacteria brevundimonas sp. sd212, paracoccus sp. pc1 (alcaligenes pc-1), and paracoccus sp. n81106 (agrobacterium aurantiacum), for astaxanthin production. each crtw gene was expressed in escherichia coli synthesizing zeaxanthin due to the presence of plasmid paccar25deltacrtx. carotenoids that accumulated in the resulting e. coli transformants were examined b ... | 2005 | 16007373 |
| [influence of nitrogen source nh4 cl concentration on curdlan production in alcaligenes faecalis]. | the effect of initial ammonium chloride level on production of curdlan in alcaligenesfaecalis was investigated. it was found that ammonium chloride was the limiting substrate for cell growth during the batch fermentation process. however, the cell growth and curdlan production could not be enhanced by solely increasing the initial ammonium chloride level. the ph drop in the broth due to the consumption of ammonium chloride also effected the cell growth and curdlan production. by simultaneously i ... | 2005 | 16013500 |
| spontaneous liberation of intracellular polyhydroxybutyrate granules in escherichia coli. | despite receiving much attention as a biodegradable substitute for conventional non-biodegradable plastics, the commercial use of polyhydroxybutyrate (phb) remains limited because of its high production cost. in order to reduce the recovery/purification cost, which forms over half of the total production cost, we have developed a new cultivation method which enables spontaneous liberation of phb by modulation of the initial inoculum size and the medium composition in recombinant escherichia coli ... | 2005 | 16024232 |
| ligand loop effects on the free energy change of redox and ph-dependent equilibria in cupredoxins probed on amicyanin variants. | in this work, we have determined the thermodynamic parameters of the reduction of four different variants of thiobacillus versutus amicyanin by electrochemical techniques. in addition, the thermodynamic parameters were determined of the low-ph conformational change involving protonation of the c-terminal histidine ligand and the concomitant dissociation of this histidine from the cu(i) ion. in these variants, the native c-terminal loop containing the cys, his, and met copper ligands has been rep ... | 2005 | 16026167 |
| amino acids in positions 48, 52, and 73 differentiate the substrate specificities of the highly homologous chlorocatechol 1,2-dioxygenases cbna and tcbc. | chlorocatechol 1,2-dioxygenase (ccd) is the first-step enzyme of the chlorocatechol ortho-cleavage pathway, which plays a central role in the degradation of various chloroaromatic compounds. two ccds, cbna from the 3-chlorobenzoate-degrader ralstonia eutropha nh9 and tcbc from the 1,2,4-trichlorobenzene-degrader pseudomonas sp. strain p51, are highly homologous, having only 12 different amino acid residues out of identical lengths of 251 amino acids. but cbna and tcbc are different in substrate ... | 2005 | 16030237 |
| in vitro evaluation of efficacy of 5 methods of disinfection on mouthpieces and facemasks contaminated by strains of cystic fibrosis patients. | home-nebulizers are a potential source of bacterial infection of the respiratory tract in patients suffering from cystic fibrosis. recommendations for disinfecting this equipment are often arbitrary and sometimes contradictory. | 2005 | 16046195 |
| tn5393d, a complex tn5393 derivative carrying the per-1 extended-spectrum beta-lactamase gene and other resistance determinants. | in alcaligenes faecalis fl-424/98, a clinical isolate that produces the per-1 extended-spectrum beta-lactamase, the bla(per-1) gene was found to be carried on a 44-kb nonconjugative plasmid, named pfl424, that was transferred to escherichia coli by electroporation. investigation of the genetic context of the bla(per-1) gene in pfl424 by means of a combined cloning and pcr mapping approach revealed that the gene is associated with a transposonlike element of the tn3 family. this 14-kb element is ... | 2005 | 16048938 |
| the first crystal structure of a family 31 carbohydrate-binding module with affinity to beta-1,3-xylan. | here, we present the crystal structure of the family 31 carbohydrate-binding module (cbm) of beta-1,3-xylanase from alcaligenes sp. strain xy-234 (alccbm31) determined at a resolution of 1.25a. the alccbm31 shows affinity with only beta-1,3-xylan. the alccbm31 molecule makes a beta-sandwich structure composed of eight beta-strands with a typical immunoglobulin fold and contains two intra-molecular disulfide bonds. the folding topology of alccbm31 differs from that of the large majority of other ... | 2005 | 16061225 |
| characterization of pseudomonas species isolated from the rhizosphere of plants grown in serozem soil, semi arid region of uzbekistan. | collections of native pseudomonas spp. are kept at the ncam of uzbekistan. some of those organisms were isolated from the rhizosphere of cotton, wheat, corn, melon, alfalfa, and tomato grown in field locations within a semi-arid region of uzbekistan. strains used for this study were pseudomonas alcaligenes, p. aurantiaca, p. aureofaciens, p. denitrificans, p. mendocina, p. rathonis, and p. stutzeri. some of the pseudomonads have been characterized in this report. these strains produced enzymes, ... | 2005 | 16075145 |
| release of an enantioselective nitrilase from alcaligenes faecalis mtcc 126: a comparative study. | nitrilases constitute an important class of hydrolases, however, cheap and ready availability of enzyme sources limit their practical synthetic applications. the present investigation was directed to compare the applicability of various physical cell disintegration methods namely, solid shear, liquid shear and sonication, for the release of an enantioselective nitrilase from alcaligenes faecalis mtcc 126. different parameters associated with each method were optimized in order to ensure maximal ... | 2005 | 16080003 |
| calorimetric and spectroscopic investigations of the thermal denaturation of wild type nitrite reductase. | nitrite reductase (nir) is a multicopper protein, with a trimeric structure containing two types of copper site: type 1 is present in each subunit whereas type 2 is localized at the subunits interface. the paper reports on the thermal behaviour of wild type nir from alcaligenes faecalis s-6. the temperature-induced changes of the copper centres are characterized by optical spectroscopy and electron paramagnetic resonance spectroscopy, and by establishing the thermal stability by differential sca ... | 2005 | 16085470 |
| nitrification and denitrification in high-strength ammonium by alcaligenes faecalis. | alcaligenes faecalis sp. no. 4, that has the ability of heterotrophic nitrification and aerobic denitrification in high-strength ammonium at about 1200 mg-n/l, converted about one-half of removed nh4+-n to intracellular nitrogen and nitrified only 3% of the removed nh4+. from the nitrogen balance, 40-50% of removed nh4+-n was estimated to be denitrified. production of n2 was confirmed by gc-ms and 90% of denitrified products was n2. the maximum ammonium removal rate, 29 mg-n/l h and its denitrif ... | 2005 | 16086258 |
| burkholderia and emerging pathogens in cystic fibrosis. | persons with cystic fibrosis (cf) are susceptible to respiratory tract infection with a variety of bacterial species. among these are several species in the genus burkholderia (including members of the b. cepacia complex, b. gladioli, and b. pseudomallei), stenotrophomonas maltophilia, achromobacter ( alcaligenes) xylosoxidans, certain ralstonia species, and species within the new genus pandoraea. enterobacteriaceae and several other rather unusual or novel species also can be found. many of the ... | 2003 | 16088584 |
| alcaligenes faecalis subsp. phenolicus subsp. nov. a phenol-degrading, denitrifying bacterium isolated from a graywater bioprocessor. | a gram (-) coccobacillary bacterium, j(t), was isolated from a graywater bioprocessor. 16s rrna and biochemical analysis has revealed strain j(t) closely resembles alcaligenes faecalis atcc 8750t and a. faecalis subsp. parafaecalis dsm 13975t, but is a distinct, previously uncharacterized isolate. strain j(t), along with the type strain of a. faecalis and its previously described subspecies share the ability to aerobically degrade phenol. the degradation rates of phenol for strain j(t) and refer ... | 2005 | 16094869 |
| [alcaligenes xylosoxidans--a pathogen of chronic ear infection]. | we discuss a case of a 48-year old man admitted to our department with symptoms of chronic ear infection, that didn't answer to typical conservative treatment. the culture of the discharge was positive for alcaligenes xylosoxidans. | 2005 | 16095102 |
| effects of paraoxon and ethylparathion on choline oxidase from alcaligenes species: inhibition and denaturation. | the kinetics and thermodynamics of the effects of paraoxon (pox) and ethylparathion (epa) on choline oxidase (chox) were studied. lineweaver-burk plots of initial velocity data showed a parallel pattern indicating uncompetitive inhibition versus choline. the inhibition constant (k(i)) obtained from the secondary plots for pox and epa were 0.14+/-0.01 and 0.48+/-0.05 mm, respectively, suggesting that pox is a more potent inhibitor of chox than epa. uv absorption was used to monitor the denaturati ... | 2005 | 16102808 |
| postoperative endophthalmitis due to an unusual pathogen: alcaligenes faecalis. | 2006 | 16138111 | |
| expression and purification of penicillin g acylase enzymes from four different micro-organisms, and a comparative evaluation of their synthesis/hydrolysis ratios for cephalexin. | several genes for the enzyme penicillin g acylase, as isolated from four different micro-organisms (alcaligenes facaelis, escherichia coli, kluyvera cryocrescens or providencia rettgeri) were modified at their carboxy-termini to include his-tag fusions, then were expressed from the plasmid pet-24a(+) in e. coli jm109(de3) cells. all fusion proteins were next purified to homogeneity in a single step by agar-based co-ida chromatography, and were then evaluated as catalysts for the synthesis of cep ... | 2006 | 16139515 |
| isolation and characterization of a chlorpyrifos and 3,5,6-trichloro-2-pyridinol degrading bacterium. | a bacterium, isolated from contaminated soils around a chemical factory and named strain dsp3 was capable of biodegrading both chlorpyrifos and 3,5,6-trichloro-2-pyridinol. based on the results of phenotypic features, phylogenetic similarity of 16s rrna gene sequences, dna g+c content, and dna homology between strain dsp3 and reference strains, strain dsp3 was identified as alcaligenes faecalis. chlorpyrifos was utilized as the sole source of carbon and phosphorus by strain dsp3. we examined the ... | 2005 | 16143458 |
| intestinal protozoa and associated bacteria in captive houbara bustards (chlamydotis undulata) in the united arab emirates. | a protozoal survey was carried out in 114 captive houbara bustards (chlamydotis undulata) in the united arab emirates. trichomonas gallinarum, chilomastix gallinarum, giardia spp. and lophomonas spp. were detected from faecal samples of 49 (43%) captive houbara bustards. culture and identification studies were carried out to assess associated bacteria. the bacteria isolated from birds negative to protozoa included enterococcus spp., aerococcus spp., micrococci spp., proteus spp., enterobacter sp ... | 1999 | 16147554 |
| presence of two different active nirs nitrite reductase genes in a denitrifying thauera sp. from a high-nitrate-removal-rate reactor. | the nirs nitrite reductase genes were studied in two strains (strains 27 and 28) isolated from two denitrifying reactors and characterized as thauera according to their 16s rrna gene sequences. strain 28 contains a single nirs sequence, which is related to the nirs of thauera mechernichensis, and strain 27 contains two nirs sequences; one is similar to the nirs sequence from thauera mechernichensis (gene 2), but the second one (gene 8) is from a separate clade with nirs from pseudomonas stutzeri ... | 2005 | 16151169 |
| the use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil. | the potential of using ozone for the removal of phenanthrene from several different soils, both alone and in combination with biodegradation using a microbial inoculant (pseudomonas alcaligenes pa-10), was examined. the greater the water content of the soil the less effective the ozone treatment, with air-dried soils showing the greatest removal of phenanthrene; while soils with higher levels of clay also reduced the effectiveness of the ozone treatments. however, at least a 50% reduction in phe ... | 2006 | 16153687 |
| control of expression of a periplasmic nickel efflux pump by periplasmic nickel concentrations. | there is accumulating evidence that transenvelope efflux pumps of the resistance, nodulation, cell division protein family (rnd) are excreting toxic substances from the periplasm across the outer membrane directly to the outside. this would mean that resistance of gram-negative bacteria to organic toxins and heavy metals is in fact a two-step process: one set of resistance factors control the concentration of a toxic substance in the periplasm, another one that in the cytoplasm. efficient peripl ... | 2005 | 16158236 |
| antibacterial activity of essential oils from australian native plants. | to date, of the australian essential oils, only tea tree (melaleuca alternifolia) and eucalyptus spp. have undergone extensive investigation. in this study a range of australian essential oils, including those from anethole anisata, callistris glaucophyllia, melaleuca spp. and thyptomine calycina, were assayed for in vitro antibacterial activity. m. alternifolia was also included for comparison purposes. activity was determined using standard disc diffusion assays with each oil assayed at 100%, ... | 2005 | 16161028 |
| tetrathiobacter kashmirensis gen. nov., sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in jammu and kashmir, india. | twelve chemolithotrophic strains were isolated from temperate orchard soil on reduced sulfur compounds as energy and electron sources and characterized on the basis of their physiological properties and ability to oxidize various reduced sulfur compounds. the new isolates could oxidize tetrathionate as well as thiosulfate, and oxidation of the latter involved conversion of thiosulfate to tetrathionate followed by its accumulation and eventual oxidation to sulfate, manifested in the production of ... | 2005 | 16166666 |
| changing bacterial isolates and antibiotic sensitivities of purulent dacryocystitis. | to examine the current spectrum of bacterial isolates in cases of chronic dacryocystitis and dacryoabcess and to determine their antibiotic sensitivities. | 2005 | 16191795 |
| characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by alcaligenes faecalis no. 4. | alcaligenes faecalis no. 4 has heterotrophic nitrification and aerobic denitrification abilities. by taking the nitrogen balance under different culture conditions, 40-50% of removed nh4+-n was denitrified and about one-half of removed nh4+-n was converted to intracellular nitrogen. the maximum ammonium removal rate of no. 4 (28.9 mg-n/l/h) and its denitrification rate at high-strength nh4+-n of about 1200 ppm in aerated batch experiments at a c/n ratio of 10 were 5-40 times higher than those of ... | 2005 | 16198262 |
| surface active properties of bacterial strains isolated from petroleum hydrocarbon-bioremediated soil. | two bacterial strains identified as ralstonia picketti (bp 20) and alcaligenes piechaudii (czor l-1b) were isolated from petroleum hydrocarbon-contaminated soil following bioremediation treatment. the surface active properties, e.g. surface tension, emulsification and foamability of their culture filtrates were evaluated. bacterial cell-surface hydrophobicity (bah) as measured by analyzing cell affinity towards aliphatic and aromatic compounds was also determinated. the bacteria grew in liquid c ... | 2005 | 16209110 |
| a case of epidemic keratoconjunctivitis complicated by alcaligenes xylosoxidans infection. | to report a case of epidemic keratoconjunctivitis complicated by alcaligenes xylosoxidans. | 2005 | 16209287 |
| a metal ion as a cofactor attenuates substrate inhibition in the enzymatic production of a high concentration of d-glutamate using n-acyl-d-glutamate amidohydrolase. | n-acyl-d-glutamate amidohydrolase (d-agase) was inhibited by 94 % when 1 mol/l n-acetyl-dl-glutamate was used as a substrate. the addition of 1 mm co2+ stabilized d-agase. moreover, the substrate inhibition was weakened to 88% with the addition of 0.4 mm co2+ to the reaction mixture. although d-agase is a zinc-metalloenzyme, the addition of zn2+ from 0.01 to 10 mm did not increase the d-glutamic acid production in the saturated substrate. under optimal conditions, 0.38 m d-glutamic acid was obta ... | 2005 | 16215833 |
| effect of the applied organic load rate on biodegradable polymer production by mixed microbial cultures in a sequencing batch reactor. | this article studies the operation of a new process for the production of biopolymers (polyhydroxyalkanoates, phas) at different applied organic load rates (olrs). the process is based on the aerobic enrichment of activated sludge to obtain mixed cultures able to store phas at high rates and yields. a mixture of acetic, lactic, and propionic acids at different concentrations (in the range 8.5-31.25 gcod/l) was fed every 2 h in a sequencing batch reactor (sbr). the resulting applied olr was in th ... | 2006 | 16224790 |
| effect of modifying metabolic network on poly-3-hydroxybutyrate biosynthesis in recombinant escherichia coli. | the regulatory mechanism for poly-3-hydroxybutyrate (phb) biosynthesis in recombinant escherichia coli is markedly different from that of ralstonia eutropha (formerly, alcaligenes eutrophus) since the former efficiently synthesizes phb during growth without any nutrient limitation. to analyze how the central metabolic pathways should be balanced with pathways necessary for cell growth and phb formation, a stoichiometric model was developed to predict the theoretical maximum phb production capaci ... | 1999 | 16232536 |