Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| design and characterization of helical peptides that inhibit the e6 protein of papillomavirus. | the e6 protein from hpv type 16 binds proteins containing a seven-residue leucine-containing motif. previous work demonstrated that peptides containing the consensus sequence are a mixture of alpha-helix and unstructured conformations. to design monomeric e6-binding peptides that are stable in aqueous solution, we used a protein grafting approach where the critical residues of the e6-binding motif of e6-associated protein, e6ap, lqellge, were incorporated into exposed helices of two stably folde ... | 2004 | 15182185 |
| prostitution and other cofactors in preinvasive and invasive lesions of the cervix. | to examine risk factors for preinvasive and invasive lesions of the cervix in venezuelan female sex workers (fsw). | 2004 | 15191449 |
| technology evaluation: hpv vaccine (quadrivalent), aventis pasteur msd/csl. | csl, licensee of uniquest's hpv technology, and aventis pasteur msd (a joint venture between merck & co and aventis) are jointly developing a vaccine for the potential prophylaxis of genital warts and cervical cancer caused by human papilloma virus infection. enrollment for a phase iii trial has been completed. | 2004 | 15195933 |
| focal epithelial hyperplasia (heck's disease): report of two cases with pcr detection of human papillomavirus dna. | focal epithelial hyperplasia (feh) (heck's disease) is essentially a benign oral infection produced by the human papillomavirus (hpv). although this condition is known to exist in numerous populations and ethnic groups, it is relatively rare in south-east asia. the following report is based on two cases of adult feh with histopathological features in favour of the disease. in addition, polymerase chain reaction was performed to detect the presence of hpv dna in the lesions in order to confirm th ... | 2004 | 15196147 |
| association of hpv infection with prognosis after neoadjuvant chemotherapy in advanced uterine cervical cancer. | whether the human papillomavirus (hpv) status of the tumor affects the sensitivity to neoadjuvant chemotherapy, and the prognosis in advanced uterine cervical cancer (figo stage iii or higher) remains unknown. we examined the hpv status of 43 patients who had received cddp therapy by balloon-occluded arterial infusion (boai), as neoadjuvant chemotherapy for advanced uterine cervical cancer (squamous cell carcinoma) stage iii or higher. dna was extracted from formalin-fixed, paraffin-embedded tum ... | 2004 | 15202023 |
| the human papillomavirus-18 genome is efficiently targeted by cellular dna methylation. | human papillomaviruses (hpvs) infect epithelia, including the simple and the squamous epithelia of the cervix, where they can cause cancer and precursor lesions. the molecular events leading from asymptomatic hpv infections to neoplasia are poorly understood. there is evidence that progression is modulated by transcriptional mechanisms that control hpv gene expression. here, we report the frequent methylation of hpv-18 genomes in cell culture and in situ. dna methylation is generally known to le ... | 2004 | 15207633 |
| the hpv16 e6/e7 oncogene sensitizes human ovarian surface epithelial cells to low-dose but not high-dose 5-fu and 5-fudr. | to evaluate the effect of hpv16 e6/e7 on drug sensitivity, primary human ose cells were infected with hpv16 e6/e7 expressing retrovirus and then exposed to chemotherapeutic agents. apoptosis induced by mitomycin c was dose-dependent in both primary ose and e6e7/ose cells. e6e7/ose cells were more sensitive to mitomycin c than parental ose cells. hpv16 e6/e7 also sensitized ose cells to 5-fu and its derivative 5-fudr, but only at low doses. this phenomenon was also observed in cervical cancer cel ... | 2004 | 15207728 |
| immunohistochemical staining for p16 and p53 in premalignant and malignant epithelial lesions of the vulva. | two distinct types of vulvar squamous cell carcinomas and their precursors, vulvar intraepithelial neoplasias (vin), which differ in terms of clinical presentation and behavior, have been delineated. human papillomavirus (hpv)-associated carcinomas are of basaloid or warty type, whereas tumors unrelated to hpv are usually keratinizing and differentiated. thus, the major stratifying factor for vulvar carcinomas and vin is their etiopathogenetic relationship with hpv. however, because of technical ... | 2004 | 15213596 |
| differential expression of the human homologue of drosophila discs large oncosuppressor in histologic samples from human papillomavirus-associated lesions as a marker for progression to malignancy. | high-risk hpvs play a causal role in the development of cervical cancer, and their e6 oncoproteins target h-dlg for ubiquitin-mediated proteolysis. the h-dlg oncosuppressor is associated with cell-cell interactions, and deregulation of these structures leads to defective cell adhesion, loss of cell polarity and unregulated proliferation. we evaluated the contribution of this e6 activity in the progression to malignancy in hpv infections by analyzing h-dlg expression in hpv-associated lesions. we ... | 2004 | 15221964 |
| prevalence and risk factors of human papilloma virus infection in asymptomatic women in southeastern hungary. | a survey was carried out to determine the prevalence of genital human papilloma virus (hpv) infection in asymptomatic women. the overall prevalence of hpv infection was 23%. age (under 24 years), pathological cytology were significantly associated with exposure to hpv infection. hpv screening is important for young women, especially, where there is a pathological cytological diagnosis. | 2004 | 15223174 |
| ano-genital warts in children: sexual abuse or not? | child sexual abuse is a problem that affects many americans. the diagnosis of sexual abuse is typically made on the basis of the child's history. objective evidence of sexual abuse, including abnormal physical findings noted on physical examination or the presence of a sexually transmitted disease, are rare. ano-genital warts are one of the most common sexually transmitted diseases found in adults. however, is the presence of ano-genital warts in children evidence of sexual abuse? the link betwe ... | 2004 | 15224040 |
| primary carcinoma of the vagina: factors influencing the age at diagnosis. the radiumhemmet series 1956-96. | the objective to this retrospective study of 341 cases of primary carcinoma of vagina (pcv) diagnosed between 1956 and 1996 was to find whether epidemiological, clinical, and histopathological variables were related to the age at diagnosis of patients with pcv. the univariate statistical analysis showed that younger age at diagnosis significantly correlated with a history of cervical dysplasia, hysterectomy, gynecological infections, and tumors located in the upper part of the vagina, whereas ol ... | 2004 | 15228423 |
| photodynamic therapy and endoscopic metal stent placement for esophageal papillomatosis associated with squamous cell carcinoma. | esophageal squamous papillomatosis is a rare condition associated with human papilloma virus infection and has been complicated by the development of squamous cell carcinoma. photodynamic therapy using porfimer sodium has been used for the treatment of esophageal cancer but has not been utilized in the treatment of esophageal squamous papillomatosis. we report here the first case of papillomatosis and obstructing squamous cell carcinoma of the esophagus palliated with porfimer sodium photodynami ... | 2004 | 15230738 |
| psittacid herpesviruses associated with mucosal papillomas in neotropical parrots. | mucosal papillomas are relatively common lesions in several species of captive neotropical parrots. they cause considerable morbidity and in some cases, result in mortality. previous efforts to identify papillomavirus dna and proteins in these lesions have been largely unsuccessful. in contrast, increasing evidence suggests that mucosal papillomas may contain psittacid herpesviruses (pshvs). in this study, 41 papillomas from 30 neotropical parrots were examined by pcr with pshv-specific primers. ... | 2004 | 15231383 |
| [treatment of cutaneous human papilloma virus, poxvirus and herpes simplex virus infections with topical cidofovir in hiv positive patients]. | cidofovir (vistide) is an antiviral marketed for the treatment of cytomegalovirus retinitis. clinical efficacy has been reported with its broad antiviral spectrum that includes poxvirus, human papilloma virus and herpes simplex. in immunodepressed patients, these infectious dermatoses are often recurrent and resistant. in an open study, we assessed the efficacy and clinical tolerance of cidofovir gel at 1 p. 100. | 2004 | 15235531 |
| chromosomal instability and marker chromosome evolution in oral squamous cell carcinoma. | squamous cell carcinoma of the head and neck and its subset, oral squamous cell carcinoma (oscc), arise through a multistep process of genetic alterations as a result of exposure to environmental agents, such as tobacco smoke, alcoholic beverages, and viruses, including human papillomavirus. we and others have shown that the karyotypes of oscc are near-triploid and contain multiple structural and numerical abnormalities. however, despite a background of clonal chromosomal aberrations, individual ... | 2004 | 15236315 |
| persistent human papilloma virus infection as an indicator of risk of recurrence of high-grade cervical intraepithelial neoplasia treated by the loop electrosurgical excision procedure. | to evaluate the recurrence rate of high-grade cervical intraepithelial neoplasia (cin) treated by the loop electrosurgical excision procedure (leep) according to the persistence of human papilloma virus (hpv) infection. | 2004 | 15237586 |
| vaccine and antibody-directed t cell tumour immunotherapy. | clearer evidence for immune surveillance in malignancy and the identification of many new tumour-associated antigens (taas) have driven novel vaccine and antibody-targeted responses for therapy in cancer. the exploitation of active immunisation may be particularly favourable for taa where tolerance is incomplete but passive immunisation may offer an additional strategy where the immune repertoire is affected by either tolerance or immune suppression. this review will consider how to utilise both ... | 2004 | 15238242 |
| changes to peptide structure, not concentration, contribute to expansion of the lowest avidity cytotoxic t lymphocytes. | the efficient in vitro expansion of antigen-specific cd8+ cytotoxic t lymphocytes (ctl) for use in adoptive immunotherapy represents an important clinical goal. furthermore, the avidity of expanded ctl populations often correlates closely with clinical outcome. in our study, high-avidity ctl lines could be expanded ex vivo from an antigen-primed animal using low peptide concentration, and intermediate peptide concentrations favored the generation of lower avidity ctl. further increases in peptid ... | 2004 | 15240746 |
| how does human papillomavirus contribute to head and neck cancer development? | 2004 | 15240772 | |
| in vitro papillomavirus capsid assembly analyzed by light scattering. | pentamers of the l1 major capsid protein of human papillomavirus (hpv type 11) were purified after expression in e. coli and analyzed for the kinetics of in vitro capsid self-assembly using multi-angle light scattering (mals). pentamers self-assembled into capsid-like structures at a rate that was a function of protein concentration. the kinetics of capsid formation were sigmoidal with a concentration-dependent lag phase, followed by a rapid increase in polymerization. nucleation size and the ra ... | 2004 | 15246271 |
| early assessment of the efficacy of a human papillomavirus type 16 l1 virus-like particle vaccine. | a post hoc analysis was performed using combined data from two phase i tolerability/immunogenicity studies of monovalent human papillomavirus type 11 (hpv11) or hpv16 l1 virus-like particle (vlp) vaccines. the goal was to determine if the hpv16 l1 vlp vaccine protected against hpv16 infection. vaccine or placebo was given at 0, 2 and 6 months. hpv16 infection was defined by positive polymerase chain reaction (pcr) results following vaccination. the incidence of hpv infection was observed to be 0 ... | 2004 | 15246630 |
| therapeutic vaccines for chronic infections. | therapeutic vaccines aim to prevent severe complications of a chronic infection by reinforcing host defenses when some immune control, albeit insufficient, can already be demonstrated and when a conventional antimicrobial therapy either is not available or has limited efficacy. we focus on the rationale and challenges behind this still controversial strategy and provide examples from three major chronic infectious diseases- human immunodeficiency virus, hepatitis b virus, and human papillomaviru ... | 2004 | 15247470 |
| immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase. | human telomerase reverse transcriptase (htert) is catalytic subunit of human telomerase. | 2004 | 15250834 |
| increased frequency of bacterial vaginosis and chlamydia trachomatis in pregnant women with human papillomavirus infection. | the aim of this study was to verify the presence of bacterial vaginosis (bv), candida sp, chlamydia trachomatis, neisseria gonorrhoeae, to determine the prevalence of tobacco use and measure vaginal ph (vph) in pregnant women with (n = 26) and without (n = 26) human papillomavirus (hpv) infection, and make comparisons between these 2 groups. hpv, c. trachomatis and n. gonorrhoeae were diagnosed using hybrid capture, bv using clinical criteria, and candida sp via cultures. a digital ph meter was ... | 2004 | 15256825 |
| human leukocyte antigen class ii alleles and natural history of hpv 2/27/57-induced common warts. | epidemiological studies have demonstrated an association between hla-dqb1*03 alleles and the risk of cervical cancer induced by human papillomavirus (hpv). as persistence of hpv infection is required for developing cervical cancer, we wanted to elucidate the role of hla-class ii allele polymorphisms in the persistence of common warts induced by hpv 2, hpv 27 or hpv 57. therefore, we determined the distribution of hla-dqa1, -dqb1, and -drb1 alleles in 71 patients presenting with hpv 2/27/57-induc ... | 2004 | 15257408 |
| codon optimization of the human papillomavirus 11 (hpv 11) l1 gene leads to increased gene expression and formation of virus-like particles in mammalian epithelial cells. | the 505 amino acid l1 protein of the human papillomavirus type 11 (hpv 11) is the major capsid polypeptide that has been shown to self-assemble into virus-like particles (vlps) in vivo and in vitro. while l1 is essential for viral infection, expression studies in mammalian cells have been hampered by different codon preference between the virus and its host. to optimize l1 gene expression in mammalian cells, we converted wild-type hpv 11 l1 (11 l1wt) codons to those more common in human genes. t ... | 2004 | 15262495 |
| prediction of class i t-cell epitopes: evidence of presence of immunological hot spots inside antigens. | processing and presentation of major histocompatibility complex class i antigens to cytotoxic t-lymphocytes is crucial for immune surveillance against intracellular bacteria, parasites, viruses and tumors. identification of antigenic regions on pathogen proteins will play a pivotal role in designer vaccine immunotherapy. we have developed a system that not only identifies high binding t-cell antigenic epitopes, but also class i t-cell antigenic clusters termed immunological hot spots. | 2004 | 15262812 |
| condylomata acuminata of the neovagina in a hiv-seropositive male-to-female transsexual. | we present an unusual case of condylomata acuminata arising in the transplanted skin of a neovagina in a male-to-female transsexual. the neovagina had been constructed using a penile and a scrotal skin flap. resection of the larger condylomata was performed; then all visible lesions were ablated by electrovaporization. microsocpic examination and dna hybridization revealed condylomata acuminata due to human papillomavirus type 16, 31, and 33 infection. | 2004 | 15263800 |
| human papillomavirus genotypes in cervical cancers in mozambique. | the distribution of human papillomavirus (hpv) types in cervical cancers is essential for design and evaluation of hpv type-specific vaccines. to follow up on a previous report that hpv types 35 and 58 were the dominant hpv types in cervical neoplasia in mozambique, the hpv types in a consecutive case series of 74 invasive cervical cancers in mozambique were determined. the most common worldwide major oncogenic hpv types 16 and 18 were present in 69 % of cervical cancers, suggesting that a vacci ... | 2004 | 15269357 |
| bilateral bowen's disease. | multiple bowen's disease may be difficult to differentiate from bowenoid papulosis because of its clinicopathological resemblance to bowenoid papulosis. we experienced a case of bilaterally and symmetrically developed multiple bowenoid lesions in a 71-year-old man previously diagnosed as having chronic lymphocytic lymphoma (cll). based on histological findings and the results of human papillomavirus examinations, we finally diagnosed this case as bilateral bowen's disease. we speculate that the ... | 2004 | 15270896 |
| prevalence of human papillomavirus type 16 variants in the federal district, central brazil. | we report the prevalence of human papillomavirus type 16 (hpv-16) variants in women with cervical lesions from the federal district, central brazil. we analyzed 34 hpv-16 samples, identifying the sequence variations of e6 and l1 genes and correlating variant frequency with disease status. the most prevalent hpv-16 variant was the european (50%), followed by asian-american (41.2%), african-1 (5.9%), and african-2 (2.9%). european and non-european variants appeared in equal frequencies among the c ... | 2004 | 15273800 |
| development of a dna vaccine targeting human papillomavirus type 16 oncoprotein e6. | human papillomavirus (hpv), particularly type 16 (hpv-16), is present in more than 99% of cervical cancers. the hpv oncoproteins e6 and e7 are constantly expressed and therefore represent ideal targets for hpv vaccine development. we previously developed dna vaccines encoding calreticulin (crt) linked to hpv-16 e7 and generated potent e7-specific cd8(+) t-cell immune responses and antitumor effects against an e7-expressing tumor. since vaccines targeting e6 also represent an important strategy f ... | 2004 | 15280455 |
| a hect e3 ubiquitin-protein ligase with sequence similarity to e6ap does not target p53 for degradation in the softshell clam (mya arenaria). | numerous reports have raised the level of national concern that chemicals found in the environment may have adverse effects on the health of humans and wildlife. environmental exposure to pollutants, such as dioxin, has been implicated in gonadal tumor formation in maine softshell clams (mya arenaria). prevalence of these tumors is as high as 40% in some populations. although their etiology is still unknown, investigations into the mechanisms of tumor formation have revolved around a hypothesis ... | 2004 | 15288542 |
| relationship between hpv typing and the status of g2 cell cycle regulators in cervical neoplasia. | we examined human papillomavirus (hpv) typing and the status of atm, chk2, cdc25c, cdc2 and cyclinb1 in cervical intraepithelial neoplasia (cin) and invasive cancer (ic). a total of 93 samples [normal: 10; cin: 34 (cini:9, cinii:12, ciniii:13); ic: 49 (stage i:10, stage ii:21, stage iii:15, stage iv:3)] were included in this study. hpv status was evaluated by the pcr non-radioactive hpv detection system. we analyzed atm, chk2, cdc25c, cdc2 and cyclinb1 protein expression by immunohistochemistry. ... | 2004 | 15289842 |
| human papillomavirus type-16 virus-like particles activate complementary defense responses in key dendritic cell subpopulations. | human papillomavirus type-16 (hpv16) l1 virus-like particles (vlps) activate dendritic cells (dcs) and induce protective immunity. in this study, we demonstrate, using global gene expression analysis, that hpv16 vlps produce quite distinct innate responses in murine splenic dc subpopulations. while hpv16 vlps increase transcription of ifn-gamma and numerous th1-related cytokines and chemokines in cd8alpha(+)cd11c(+) dcs, cd4(+)cd11c(+) dcs up-regulate only type i ifn and a different set of th2-a ... | 2004 | 15294979 |
| human papillomavirus genotyping by a polymerase chain reaction-based genechip method in cervical carcinoma treated with neoadjuvant chemotherapy plus radical surgery. | the aim of this study was to evaluate the accuracy of human papillomavirus (hpv) genotyping by a polymerase chain reaction (pcr)-based genechip method and to determine the prognostic value of hpv genotype in bulky stage ib or iia cervical carcinoma treated with neoadjuvant chemotherapy (nac) and radical surgery. a total of 149 patients had adequate tissue for the study. the spf1/gp6+ primers were used to amplify a 184 bp fragment. the amplimers were submitted for direct sequencing and hybridizat ... | 2004 | 15304160 |
| condom use promotes regression of cervical intraepithelial neoplasia and clearance of human papillomavirus: a randomized clinical trial. | 2004 | 15305390 | |
| ectopic expression of nonliganded retinoic acid receptor beta abrogates ap-1 activity by selective degradation of c-jun in cervical carcinoma cells. | expression of the nuclear retinoic acid receptor beta2 (rarbeta2) gene is often disturbed in cervical carcinoma cells. one important mechanism by which rarbeta2 can exert growth inhibitory function is based on its ability to repress the ap-1 transcription factor in a ligand-dependent manner. because less is known about the biological effects of rarbeta in the absence of ligand, the corresponding cdna was stably introduced into hpv18-positive hela cervical carcinoma cells. in the present study we ... | 2004 | 15308638 |
| biochip to detect human papillomavirus in mexico. | 2004 | 15309780 | |
| [preliminary study on the relationship between loads of human papillomavirus in cervical carcinoma and cervical intraepithelial neoplasia]. | to explore the relationship between loads of human papillomavirus in cervical carcinoma and cervical intraepithelial neoplasia. | 2004 | 15312325 |
| iscomatrix adjuvant: an adjuvant suitable for use in anticancer vaccines. | human papillomavirus type 16 (hpv16) e6 and e7 oncoproteins are associated with cervical cancer development and progression and can therefore be used as target antigens for cancer immunotherapy. in this study we evaluated the immunogenicity in mice, of different vaccine formulations using recombinant hpv16 derived e6e7 or e7gst fusion proteins. when co-administered with iscomatrix adjuvant, these e6e7 proteins consistently induced e7 specific ctl, in vivo tumor protection, antibody and dth respo ... | 2004 | 15315854 |
| antiapoptotic effects of estrogen in normal and cancer human cervical epithelial cells. | the present study investigated the antiapoptotic effects of estrogen in normal and cancer human cervical cells and the mechanisms involved. baseline apoptosis in human cervical epithelial cells is mediated predominantly by p2x7-receptor-induced, ca(2+)-dependent activation of the mitochondrial (caspase-9) pathway. treatment with 10 nm 17beta-estradiol blocked apoptosis induced by the p2x7-receptor ligands atp and 2',3'-0-(4-benzoylbenzoyl)-atp in normal human cervical epithelial cells (heces) an ... | 2004 | 15319352 |
| concomitant chlamydia trachomatis and human papilloma virus infection cannot be attributed solely to sexual behaviour. | 2004 | 15322933 | |
| human papillomavirus infection and loss of heterozygosity in esophageal squamous cell carcinoma. | the incidence/mortality rates of esophageal squamous cell carcinoma (escc) vary widely in different parts of china. human papillomavirus (hpv) infection is considered a possible risk factor. loss of heterozygosity (loh) analysis on 87 escc specimens collected from three different areas of china showed lower frequency of loh at marker d3s1621 in linxian, an area with exceptionally high incidence of escc but low hpv infection rate. hpv-positive escc from hong kong, but not sichuan, had higher freq ... | 2004 | 15327839 |
| [dendritic cells present particulate e7 protein of human papillomavirus and induce strong immunity]. | to investigate the feasibility of using dendritic cell (dc)-beads-antigen (ag) as novel cancer vaccine form with e7 as the target antigen. | 2004 | 15329282 |
| squamous cell carcinoma in situ arising at the exit site of a tunneled catheter. | squamous cell carcinoma (scc) is a well-known complication of immunosuppression associated with organ transplantation. it may arise de novo or from previously existing in situ lesions (bowen's disease). concurrent human papilloma virus infection often has an etiologic role, and scc may follow an aggressive course in immunosuppressed patients. the authors describe a liver transplant patient in whom end-stage renal disease developed. she underwent tunneled catheter placement followed by arterioven ... | 2004 | 15332239 |
| effects of intrinsic variables on release of sodium dodecyl sulfate from a female controlled drug delivery system. | the release profile of sodium dodecyl sulfate (sds), a potent microbicide, from a female controlled drug delivery system (fcdds) made of carbopol 934p and hydroxypropyl methylcellulose (hpmc) was evaluated using a newly developed in vitro simulant vaginal system (svs). the major parameters involved in the release profiles of sds were categorized as: (1) formulation variables (total loading weight of intravaginal delivery systems, sds loading doses in intravaginal delivery systems); (2) intrinsic ... | 2004 | 15336392 |
| interleukin-2 (il-2) receptor-betagamma signalling is activated by c-kit in the absence of il-2, or by exogenous il-2 via jak3/stat5 in human papillomavirus-associated cervical cancer. | activation of the interleukin-2 receptor (il-2r) induces signalling cascades promoting t cell proliferation. however, signal transduction pathways triggered in il-2r-expressing solid tumours are unknown. this report shows that human papillomavirus (hpv)-associated cervical cancer cells express an il-2r composed of beta and gamma chains (il-2rbetagamma), and that il-2-mediated activation increases the phosphorylation of jak3 and stat5, stimulating cell proliferation. interestingly, endogenous il- ... | 2004 | 15337523 |
| [laryngeal papillomatosis. role of human papilloma virus, perspectives of the diagnosis and treatment]. | 2004 | 15341014 | |
| pap test in a high-risk population comparison of conventional and liquid-base cytology. | liquid-based cytology (lbc) is believed to have better sensitivity than conventional smears (css) and offers the possibility to perform molecular assay. the goal of this work was to study the performance of cs and lbc in a high-risk population and to compare the results with the hybrid capture (hc) ii for high-risk human papillomavirus (hpv). samples were collected from selected women with clinical suspicions of low genital tract lesion at pérola biygnton hospital (são paulo, brazil). after cs p ... | 2004 | 15349987 |
| high frequency of hpv16-associated head and neck squamous cell carcinoma in the puerto rican population. | recent evidence has accumulated suggesting that human papillomavirus (hpv) plays a role in the development of head and neck squamous cell carcinoma (hnscc). hpv16 is the most common of the hpv subtypes associated with oral and laryngeal malignancies. this study estimated the prevalence of hpv16 dna in puerto rican patients with hnscc. | 2004 | 15350023 |
| a comparison of modified monoprep2 of liquid-based cytology with thinprep pap test. | the purpose of this study is to evaluate a modified monoprep2 (mp) of liquid-based cytology (lbc) to search for a less expensive alternative technique usable for screening of cervical cancers. | 2004 | 15350360 |
| recurrent human papilloma virus associated lesions in an immunocompromised host: a case report. | 2004 | 15354666 | |
| effectiveness of condoms in preventing sexually transmitted infections. | in june 2000, the united states national institutes of health (nih) organized a review of the scientific evidence on the effectiveness of condoms in preventing sexually transmitted infections (stis). the review concluded that condoms were effective in protecting against transmission of hiv to women and men and in reducing the risk of men becoming infected with gonorrhoea. evidence for the effectiveness of condoms in preventing other stis was considered to be insufficient. we review the findings ... | 2004 | 15356939 |
| different methods of identifying new antigenic epitopes of human papillomavirus type 16 e6 and e7 proteins. | human papillomavirus (hpv) infection is the most common cause of sexually transmitted viral infection and is the main cause of cervical cancer. identification of hpv t-cell epitopes would be instrumental not only in our understanding of the protective immune response but also in the development of vaccines and immunotherapies. in contrast to viruses which cause systemic infection, identification of hpv epitopes is technically challenging because hpv causes a localized mucosal infection and the f ... | 2004 | 15358648 |
| prophylactic human papillomavirus vaccines: the beginning of the end of cervical cancer. | persistent infection with one of the oncogenic human papillomavirus (hpv) types is a necessity for the development of cervical cancer. by hpv vaccination, cervical cancer could become a very rare disease. two types of hpv vaccines can be distinguished: (i) therapeutic vaccines which induce cellular immunity targeted against epithelial cells infected with hpv and (ii) prophylactic vaccines inducing virus-neutralizing antibodies protecting against new but not against established infections. at pre ... | 2004 | 15361181 |
| hpv-dna, vascular space invasion, and their impact on the clinical outcome in early-stage cervical carcinomas. | the present study was designed to analyze the relationship of human papillomavirus (hpv)-dna, microvessel density, and their impact on clinical outcome in early cervical carcinoma. hpv-dna was evaluated in 171 cases of cervical carcinoma treated from 1965 to 1990. in 110 cases, the analyses could be performed. a polymerase chain reaction technique was used on paraffin-embedded specimens obtained before the start of therapy. hpv-dna of any type was detected in 78% (86/110) of all evaluable tumors ... | 2004 | 15361201 |
| human papilloma virus testing knowledge and attitudes among women attending colposcopy clinic with ascus/lgsil pap smears. | to study women's knowledge regarding the role of human papilloma virus (hpv) in cervical intraepithelial neoplasia and their attitudes toward the integration of hpv testing as part of routine follow-up of atypical squamous cell of uncertain significance/low-grade squamous intraepithelial lesion (ascus/lgsil) abnormalities. | 2004 | 15361273 |
| the hiv/aids pandemic: new ways of preventing infection in men. | nelson mandela has recently said that hiv/aids is the greatest health crisis in human history. at the end of 2001, unaids estimated that there were 40 million people living with the infection, with 5 million new cases, and 3 million deaths per year. about half the infected individuals are men, and heterosexual intercourse is the commonest route of infection. although condoms remain the first line of defence, they are unpopular in parts of africa, where the pandemic is worst, so an alternative is ... | 2004 | 15367370 |
| lung adenocarcinoma and human papillomavirus infection. | over the past three decades, the incidence of lung adenocarcinoma has increased worldwide. most individuals with lung adenocarcinoma (especially women) are nonsmokers. reported risk factors for the development of lung adenocarcinoma include cigarette smoking; exposure to cooking fumes, air pollution, second-hand smoke, asbestos, and radon; nutritional status; genetic susceptibility; immunologic dysfunction; tuberculosis infection; and asthma. human papillomavirus (hpv) infection is a known risk ... | 2004 | 15368331 |
| human papilloma virus type 16 infection and the early onset of cervical cancer. | human papilloma viruses (hpv), particularly type 16, have been associated with cervical cancer. it has been noted that the average onset of cervical cancer is occurring in younger women coupled with a higher prevalence of cervical hpv infection. however, the correlation between hpv 16 infection and the early onset of cervical cancer is still unclear. we hypothesize that hpv infection is an indicator of early onset of cervical cancer. to test this hypothesis, cervical smears from 197 women were e ... | 2004 | 15369795 |
| the relationship between syntactic structure analysis features, histological grade and high-risk hpv dna in cervical intraepithelial neoplasia. | to assess the correlation between syntactic structure analysis (ssa) features, revised dysplasia grade and the presence of high-risk human papillomavirus dna in cervical intraepithelial neoplasia (cin). | 2004 | 15371649 |
| chemotherapy and immunotherapy of tumours induced by gene-modified hpv16-transformed cells. | hpv16 e6/e7 transformed mouse kidney cells designated mk16/1/iiiabc (mk16) were modified by the insertion of a suicide gene, viz. the thymidine-kinase gene of herpes simplex virus (hsv tk). tumour induction by these cells, designated n2a, was suppressed by ganciclovir (gcv). the growth of already established tumours was partially inhibited by gcv. this effect was markedly potentiated by a single dose of cyclophosphamide (cy). ganciclovir- or gcv+ cy-cured mice were not protected against challeng ... | 2004 | 15375516 |
| hpv16-associated tumours: therapy of surgical minimal residual disease with dendritic cell-based vaccines. | dendritic cell (dc)-based vaccines are being intensively investigated for the treatment of a variety of human neoplasms. however, little attention has until now been paid to the use of dc-based vaccines for immunotherapy of tumour residua after surgery. in this communication, an animal model mimicking human hpv16-associated neoplasms was employed to examine the effect of dc-based vaccines for the treatment of surgical minimal residual tumour disease. mice were subcutaneously inoculated with syng ... | 2004 | 15375569 |
| cyclin-dependent kinase inhibitor indirubin-3'-oxime selectively inhibits human papillomavirus type 16 e7-induced numerical centrosome anomalies. | dysregulation of the centrosome duplication cycle has been implicated in tumorigenesis. our previous work has shown that the human papillomavirus type 16 (hpv-16) e7 oncoprotein rapidly induces aberrant centrosome and centriole duplication in normal human cells. we report here that hpv e7-induced abnormal centriole duplication is specifically abrogated by a small molecule cdk inhibitor, indirubin-3'-oxime (io), but not a kinase-inactive derivative. importantly, normal centriole duplication was n ... | 2004 | 15378001 |
| loss of c-abl facilitates anchorage-independent growth of p53- and rb- deficient primary mouse embryonic fibroblasts. | the c-abl tyrosine kinase is the proto-oncogene of the v-abl oncogene of the abelson murine leukemia virus. although mutational variants of c-abl can exhibit gain of function and can produce a transformed phenotype, the function of c-abl in transformation remained unclear. here, we report that the loss of c-abl facilitates transformation. c-abl-knockout mouse embryonic fibroblasts (mefs) immortalized by sv40 t antigen acquired anchorage-independent growth, and by constructing mutational variants ... | 2004 | 15378021 |
| risk of high-grade squamous intraepithelial lesion in hiv-infected adolescents. | the risk of developing the human papillomavirus (hpv)-associated precancer high-grade squamous intraepithelial lesion (hsil) in human immunodeficiency virus (hiv)-infected adolescents is unknown. we examined the risk of developing hsil among adolescents with and without hiv infection. | 2004 | 15378433 |
| expression of hypoxic-inducible factor 1alpha predicts metastasis-free survival after radiation therapy alone in stage iiib cervical squamous cell carcinoma. | hypoxia-inducible factor-1alpha (hif-1alpha) is an intrinsic marker of tumor hypoxia. it has been considered that the hypoxic status reduces radiosensitivity, but the role of hif-1alpha in advanced cervical carcinoma is still unclear. the objective of this study was to clarify the impact of hif-1alpha, human papillomavirus (hpv), and other molecular factors, such as p53, bax, bcl-2, and their correlations on the outcome of patients with stage iiib cervical carcinoma in radiation therapy. | 2004 | 15380586 |
| american society of pediatric otolaryngology members' experience with recurrent respiratory papillomatosis and the use of adjuvant therapy. | to evaluate how evolving treatment technologies have affected our management of recurrent respiratory papilloma (rrp) since the last comprehensive survey of pediatric otolaryngologists in 1998. | 2004 | 15381589 |
| p16(ink4a) expression is related to grade of cin and high-risk human papillomavirus but does not predict virus clearance after conization or disease outcome. | the role of p16(ink4a) as a marker of hr-hpv and in the diagnosis of cin has been well established, but its predictive value in the clearance of the virus after cin treatment and its use as a prognostic marker of cervical cancer has not been studied. a series of 302 archival samples, including 150 squamous cell carcinomas (sccs) and 152 cin lesions, were subjected to immunohistochemical staining for p16(ink4a) and hpv testing using pcr with three primer sets (my09/11, gp5/gp6, spf). follow-up da ... | 2004 | 15381905 |
| small cell neuroendocrine carcinomas of the uterine cervix: a histological, immunohistochemical, and molecular genetic study. | small cell carcinomas of the uterine cervix are rare tumors with an aggressive behavior. although these tumors can exhibit neuroendocrine differentiation, the criteria for neuroendocrine differentiation are subjective and not well defined. in this study, the authors tentatively defined small cell neuroendocrine carcinoma (scnec) as a tumor composed of small cells with at least two of the following: argyrophilic cytoplasm, chromogranin a immunoreactivity, and synaptophysin immunoreactivity. we fo ... | 2004 | 15381906 |
| prognostic relevance of tgf-beta1 and pai-1 in cervical cancer. | cervical carcinoma is a human papilloma virus (hpv)-related immunogenic type of malignancy, in which escape of the tumor from the hosts' immune response is thought to play an important role in carcinogenesis. the multifunctional cytokine transforming growth factor-beta(1) (tgf-beta(1)) is involved in immunosuppression, stroma and extracellular matrix formation and controlling (epithelial) cell growth. the plasminogen activating (pa) system plays a key role in the cascade of tumor-associated prot ... | 2004 | 15386352 |
| hpv-associated flat penile lesions in men of a non-std hospital population: less frequent and smaller in size than in male sexual partners of women with cin. | human papillomavirus (hpv) infections and hpv-associated penile lesions are frequently found in male sexual partners of women with cervical intraepithelial neoplasia (cin). to determine the significance of these findings, we studied the prevalence of hpv and hpv associated penile lesions in a male hospital population with non-std complaints. penoscopy was performed after application of acetic acid to identify flat lesions, papular lesions, condylomata acuminata and pearly penile papules (ppps). ... | 2005 | 15386360 |
| e5 protein of human papillomavirus type 16 selectively downregulates surface hla class i. | papillomaviruses have evolved mechanisms that result in escape from host immune surveillance. the e5 protein is expressed early in papillomavirus infection in the deep layers of the infected epithelium. it is localized to the golgi apparatus (ga) and endoplasmic reticulum. the e5 protein of bovine papillomavirus (bpv) impairs the synthesis and stability of major histocompatibility (mhc) class i complexes and prevents their transport to the cell surface due to retention in the ga. here we show th ... | 2005 | 15386416 |
| human papillomavirus-32-positive extragenital bowenoid papulosis (bp) in a hiv patient with typical genital bp localization. | bowenoid papulosis (bp) is characterized by multiple maculopapular lesions of the genitalia; extragenital localization is rarely found. human papillomavirus (hpv) type 16 has been found in most cases of bp, but the other hpv genotypes associated with bp had been poorly characterized. | 2004 | 15389001 |
| adjunctive treatment with interferon alpha-2b may decrease the risk of papilloma-associated conjunctival intraepithelial neoplasm recurrence. | to report a case of bilateral papilloma virus-positive conjunctival intraepithelial neoplasm and the treatment results of using adjunctive interferon alpha-2b. | 2004 | 15448502 |
| human papillomavirus and cervical cytology in adolescents. | human papillomavirus (hpv) infection is highly prevalent in adolescent girls and young women and may be associated with substantial morbidity and mortality. hpv infection may lead to condylomata (genital warts), cervical intraepithelial neoplasia (cin), and cervical cancer. recent research focused on the virology, natural history, and sequelae of hpv infection has led to evidence-based revisions of the system for classification of cervical cytology results, recommendations for cytologic screenin ... | 2004 | 15449847 |
| lactoferrin inhibits human papillomavirus binding and uptake in vitro. | lactoferrin (lf), a member of the transferrin family, is a bi-globular protein secreted in milk, saliva, tears, seminal fluid, endocervix and vaginal secretions. lf is an important player in the defence against pathogenic microorganisms and has also been shown to have activity against several viruses including herpesvirus, adenovirus, rotavirus and poliovirus. the antiviral activity of lf is directed against the early steps of viral infection and the lf antiviral effect against herpesvirus is me ... | 2004 | 15451180 |
| identification of an hnrnp a1-dependent splicing silencer in the human papillomavirus type 16 l1 coding region that prevents premature expression of the late l1 gene. | we have previously identified cis-acting rna sequences in the human papillomavirus type 16 (hpv-16) l1 coding region which inhibit expression of l1 from eukaryotic expression plasmids. here we have determined the function of one of these rna elements, and we provide evidence that this rna element is a splicing silencer which suppresses the use of the 3' splice site located immediately upstream of the l1 aug. we also show that this splice site is inefficiently utilized as a result of a suboptimal ... | 2004 | 15452209 |
| molecular analysis of the effect of topical imiquimod treatment of hpv 2/27/57-induced common warts. | imiquimod is effective in the treatment of genital warts and clinical studies suggest activity against common warts as well. we have analyzed the effect of topical imiquimod on gene expression and virus load in human papilloma virus (hpv) 2/27/57-induced common warts. mrna was extracted from keratinocyte culture, from normal skin, from three untreated common warts and from three common warts treated topically with 5% imiquimod cream twice daily. differential gene expression was demonstrated by r ... | 2004 | 15452412 |
| immunocytochemical staining of p16ink4a protein from conventional pap test and its association with human papillomavirus infection. | the p16ink4a protein is immunocytochemically detected in liquid-based (lb) specimens as a diagnostic marker of cervical dysplasia and neoplasia. its up-regulation is promoted by high-risk human papillomavirus (hr-hpv) infection. we aimed to detect p16ink4a on conventional papanicolaou (pap) test (cpt) slides and to determine the relationship between its overexpression and hr-hpv infection. cpt and lb pap test (lbpt) slides (165 samples of each) were examined by immunocytochemical staining for p1 ... | 2004 | 15452898 |
| [occurrence of human papillomavirus is a positive factor in tonsillar cancer. reduced risk of recurrence and longer survival]. | the purpose of this article is to review the current knowledge on the status and significance of human papillomavirus (hpv) in tonsillar cancer. current data demonstrate that approximately half of all tonsillar cancer is hpv positive. moreover, patients with hpv positive cancer have a less risk of relapse and have a longer survival compared to patients with hpv negative tonsillar cancer. the longer survival is not dependent on that hpv positive tonsillar cancer is more sensitive to radiation the ... | 2004 | 15457821 |
| human keratin 14 driven hpv 16 e6/e7 transgenic mice exhibit hyperkeratinosis. | human papillomavirus type 16 (hpv16) has been known as a major causative factor for the development of uterine cervical carcinomas. to investigate the in vivo activity of hpv16 expressed in squamous epithelia, transgenic mice harboring hpv16 e6/e7 with human keratin 14 (hk14) promoter were generated. grossly, hk14 driven hpv16 e6/e7 transgenic mice exhibited multiple phenotypes, including wrinkled skin that was apparent prior to the appearance of hair in neonates, thickened ears, and loss of hai ... | 2004 | 15474555 |
| urethral condyloma acuminata following urethral instrumentation in an elderly man. | a 70-year-old man had undergone urethral dilatation with bougie for 8 months following transurethral resection of the prostate and complained papillary masses at the urethral meatus. physical and endoscopic examination revealed multiple tumors from the urethral meatus to the bulbous urethra. these tumors were resected transurethrally and 5-fu cream was instilled into urethra. microscopic examination revealed urethral condyloma acuminata. human papillomavirus types 6/11 were detected in the condy ... | 2004 | 15479306 |
| [detection of chlamydia trachomatis in cervical smear samples with determined hpv]. | chlamydia trachomatis (ct) causes infertility, ectopyc pregnancy, pelvic inflammatory disease (pid) and conjunctivitis. human papilloma virus (hpv) is a virus with broad spectrum effects. this particularly refers to hpv of high oncogenic potential (hpv - hr) because of its participation in cervical oncogenesis. the authors wanted to determine correlation between cervical hpv infection and detection of ct taken from female patient's smear of different levels of cervical intraepithelial neoplasm ( ... | 2004 | 15484852 |
| equine papillomavirus type 1: complete nucleotide sequence and characterization of recombinant virus-like particles composed of the ecpv-1 l1 major capsid protein. | equus caballus papillomavirus type 1 (ecpv-1) was isolated from a cutaneous papilloma, the most common neoplasm in horses. the complete ecpv-1 nucleotide sequence and genomic organization were determined. phylogenetic analysis showed that ecpv-1 is a close-to-root papillomavirus, with only distant relationships to the fibropapillomaviruses and the benign cutaneous papillomaviruses. to produce ecpv-1 virus-like particles (vlps), the ecpv-1 l1 major capsid protein was expressed in insect cells usi ... | 2004 | 15485669 |
| development and characterization of a rabbit corneal endothelial cell line. | to develop a rabbit corneal endothelial cell line by transducing human papilloma virus (hpv) type 16 e6 and e7 oncogenes, and to characterize the inherent biological properties of the established cell line. | 2004 | 15486767 |
| topical 5-aminolaevulinic acid-photodynamic therapy for the treatment of urethral condylomata acuminata. | electrocoagulation and laser evaporation for urethral condylomata acuminata have high recurrence rates and can be associated with urethral malformations. | 2004 | 15491431 |
| persistent hpv infection in postmenopausal age women. | persistence of human papillomavirus (hpv) is associated with an increased risk of developing cervical sil and cancer in young women. because this association in older, postmenopausal age women has received little attention, we evaluated persistence of hpv among women in this age group. | 2004 | 15491557 |
| treatment of undifferentiated vulvar intraepithelial neoplasia with 5% imiquimod cream: a prospective study of 12 cases. | to assess the efficacy of 5% imiquimod cream on undifferentiated vulvar intraepithelial neoplasia (vin), a disease caused by high-risk human papillomavirus. | 2004 | 15492184 |
| human papillomavirus genotyping and p16ink4a expression in cervical intraepithelial neoplasia of adolescents. | adolescents have high rates of human papillomavirus (hpv) infection, and persistent high-risk hpv infection can lead to the development of cervical cancer. the cyclin-dependent kinase inhibitor, p16(ink4a) is overexpressed in cervical intraepithelial neoplasia (cin), probably due to a persistent and integrated hpv infection. this study investigated p16(ink4a) expression, grades of cin, and high-risk hpv infection in adolescent cervical biopsies. biopsies were immunohistochemically stained for p1 ... | 2005 | 15492761 |
| factors associated with hpv persistence after treatment for high-grade cervical intra-epithelial neoplasia with large loop excision of the transformation zone (lletz). | human papillomavirus (hpv) persistence after high-grade cervical intra-epithelial neoplasia (cin) removal may be associated with residual lesions or risk of disease recurrence. knowledge regarding the factors associated with hpv persistence following cin treatment is still limited. the main purpose of this longitudinal study was to assess the association between characteristics of the patients and their cervical lesions with high-risk hpv-type persistence, detected by commercially available hybr ... | 2004 | 15494268 |
| retroviral vector backbone immunogenicity: identification of cytotoxic t-cell epitopes in retroviral vector-packaging sequences. | retroviral vectors are the frequently applied gene delivery vehicles for clinical gene therapy, but specificity of the immunogenicity to the protein encoded by the inserted gene of interest is a problem which needs to be overcome. here, we describe human cytotoxic t-lymphocyte (ctl) clones recognizing epitopes derived from the protein encoded by the retroviral vector backbone, which were established during the course of our attempts to generate ctls against cytomegalovirus (cmv) or human papillo ... | 2005 | 15496958 |
| [human papillomavirus (hpv) infection and its relation with squamous cell carcinoma of the mouth and oropharynx]. | to establish a connection between infection by the human papilloma virus (hpv) and development of malignant (squamous cell carcinoma) and benign lesions (epithelial hyperplasia papillomatosis and papilloma) of the oral cavity and oropharynx. | 2004 | 15499474 |
| [human papillomavirus oncogenicity and grade of anal intraepithelial neoplasia in hiv positive patients]. | we decided to evaluate if grade of anal intraepithelial neoplasia (ain) was associated to the hpv type in hiv positive patients, since this group of patients has immunodeficiency over long periods. we identified hpv types by pcr (polimerase chain reaction) and histological examination to determine the ain grade in 39 hiv positive males with anal condylomata acuminata. | 2004 | 15499480 |
| the l2 minor capsid protein of human papillomavirus type 16 interacts with a network of nuclear import receptors. | the l2 minor capsid proteins enter the nucleus twice during viral infection: in the initial phase after virion disassembly and in the productive phase when, together with the l1 major capsid proteins, they assemble the replicated viral dna into virions. in this study we investigated the interactions between the l2 protein of high-risk human papillomavirus type 16 (hpv16) and nuclear import receptors. we discovered that hpv16 l2 interacts directly with both kapbeta(2) and kapbeta(3). moreover, bi ... | 2004 | 15507604 |
| pseudovirions as specific tools for investigation of virus interactions with cells. | this chapter outlines the generation and application of human papillomavirus type 33 (hpv33) pseudovirions. the method describes (1) the construction of vaccinia viruses recombinant for the major and minor hpv capsid proteins, l1 and l2, respectively; (2) the transfection of cos7 cells with a marker plasmid replicating to high copy numbers; (3) the expression of l1 and l2 using the vaccinia virus expression system; (4) the extraction, purification, and analysis of hpv33 pseudovirions; and (5) th ... | 2005 | 15507710 |
| managing the sexually transmitted disease pandemic: a time for reevaluation. | the serious implications of the sexually transmitted disease (std) pandemic that currently challenges educators, medical practitioners and governments suggest that prevention strategies, which primarily focus on barrier protection and the management of infection, must be reevaluated and that initiatives focusing on primary prevention of behaviors predisposing individuals to std risk must be adopted. human immunodeficiency virus/acquired immunodeficiency syndrome, human papillomavirus, genital he ... | 2004 | 15507928 |
| reproductive tract infections in rural women from the highlands, jungle, and coastal regions of peru. | to define the prevalences and manifestations of reproductive tract infections (rtis) in rural peruvian women. | 2004 | 15508193 |