Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
observations on longevity and egg production in aedes (stegomyia) aegypti linn. mosquitoes infected with uganda s virus. 196214013820
genetics of ddt-resistance in several strains of aedes aegypti. 196214015907
observations on the irritability of mosquitos to ddt in uganda.the need for new investigations on the effect of insecticides on the behaviour of mosquitos was recently stressed by the who expert committee on insecticides, which recommended a provisional method for the determination of the irritability of mosquitos to insecticides for trials in the field and the laboratory. one such trial, carried out in uganda under what can be termed typical field conditions, is reported in the present paper.two strains of anopheles gambiae, one of anopheles funestus, one ...196214024193
[statistical study of biometric measurements carried out on adults of aedes aegypti l. belonging to strains from different sources]. 201314036647
serological identification of the natural hosts of aedes aegypti (l.) and some other mosquitoes (diptera, culicidae) caught resting in vegetation in kenya and uganda. 196314042652
[eradication in the americas of the african invaders, aedes aegypti and anopheles gambiae]. 196314042709
methods for mass rearing of aedes aegypti (l.). 014049192
initial field studies in upper volta with dichlorvos residual fumigant as a malaria eradication technique. 2. entomological evaluation.in mud houses in wakara, upper volta, dichlorvos dispensers installed at the rate of one dispenser per approximately 500 cubic feet (14 m(3)) produced 70% or higher average kills of adult aëdes aegypti, exposed overnight in screen wire cages suspended at levels of 6 feet (1.8 m) and 2 feet (0.6 m), for approximately three months during the wet season and four months during the dry season. average kills of 96% and 80% were obtained in the dry season at the 6-foot and 2-foot levels, respectively, ...196314056277
observations on the chemosterilant effect of an alkylating agent, thio-tepa, on wild-caught anopheles gambiae var. melas (theo.) in gambia, west africa, and on laboratory-bred a. g. gambiae giles and aedes aegypti (l.). 196314065754
[simple mathematical interpretation of the sensitivity of aedes aegypti to ddt-base insecticides]. 201314073533
dengue types 1 and 4 viruses in wild-caught mosquitoes in south india.three strains of type 1 dengue virus and two strains of type 4 dengue virus have been isolated from aedes aegypti collected in vellore, india.196414075719
aedes aegypti eradication in the americas. 196414075868
larval development of brugia ceylonensis jayewardene, 1962, in aedes aegypti, with a brief comparison of the infective larva with those of brugia spp., dirofilaria repens and artionema digitata. 196314077666
the survival of semliki forest virus in infected aedes aegypti mosquitoes stored at low temperatures. 196314085687
antimetabolites in the nutrition of aedes aegypti l. larvae. thiamine, riboflavin, and pantothenic acid antagonists. 196314101005
failure to obtain experimental transovarian transmission of west nile virus by aedes aegypti. 196314101931
coastal survey of aedes aegypti-breeding in british guiana. 196314101935
further studies on a strain of aedes aegypti susceptible to infection with sub-periodic brugia malayi. 196314101936
a preliminary cross-over value between the gene fm (filarial susceptibility, brugia malayi) and the sex locus in aedes aegypti. 196314101937
antimetabolites in the nutrition of aedes aegypti l. larvae. pyridoxine antagonists. 196314113512
experimental observations governing the choice of a test method for determining the ddt-irritability of adult mosquitos.in an attempt to determine the optimum test conditions for assessing the irritability of adult mosquitos to ddt, the author has carried out a series of experiments with two strains of aedes aegypti, one resistant and one susceptible to ddt. in these experiments a comparison was made between, on the one hand, the results obtained with the three possible test steps included in a tentative method proposed in 1960 by the who expert committee on insecticides, and, on the other hand, the results with ...014122447
yolk protein uptake in the oocyte of the mosquito aedes aegypti. l.yolk proteins are thought to enter certain eggs by a process akin to micropinocytosis but the detailed mechanism has not been previously depicted. in this study the formation of protein yolk was investigated in the mosquito aedes aegypti l. ovaries were fixed in phosphate-buffered osmium tetroxide, for electron microscopy, before and at intervals after a meal of blood. the deposition of protein yolk in the oocyte was correlated with a 15-fold increase in 140 mmicro pit-like depressions on the oo ...014126875
transmission of plasmocium gallinaceum brumpt to chicks by aedes aegypti (l.) sterilised by an alkylating agent, thiotepa. 196414132528
resistance to the chemical sterilant, apholate, in aedes aegypti.increased resistance to the sterilizing effects of apholate was observed in two colonies of aedes aegypti (l.) exposed in the larval stage of each generation to concentrations of apholate that induced about 90 to 40 percent sterility in the eggs laid by the ensuing adults.196414163772
genetics of insecticide-resistance and several visible mutants in aedes aegypti. 196414165062
[study of the relation existing in larvae (4th stage) of aedes aegypti l. between duration of exposure to an insecticide and the resulting mortality]. 201314184786
laboratory observations on the oviposition habits of aedes (stegomyia) aegypti (linnaeus). 196414212893
[are certain morphological characteristics sufficient to differenciate the varieties of aedes (stegomyia) aegypti linnaeus 1762?importance of the morphological characteristics]. 200614220591
evaluation of four insecticides as aedes aegypti larvicides. 196414245047
transmission of sindbis virus by aedes aegypti to a chick embryo cell culture system. 196514264544
a morphological study of the female reproductive system and follicular development in the mosquito aedes aegypti (l). 196514269883
maintenance of aedes aegypti in the insectary in mexico city. 196414272444
the aseptic rearing of aedes aegypti. 014274211
partial suppression of malaria parasites, and of the transmission of malaria, in aedes aegypti (l.) doubly-infected with semliki forest virus and plasmodium gallinaceum brumpt.laboratory experiments were undertaken with aedes aegypti infected with both semliki forest virus (sfv) from infant mice and plasmodium gallinaceum from fowls to determine if such double infection of mosquitos suppressed their ability to transmit the malaria parasite, a possibility suggested to explain reduction in malaria transmission in uganda in 1960 when anopheles funestus and anopheles gambiae were transmitting both malaria and o'nyongnyong virus to the african population. in general, trans ...196414278005
mosquito transmission of a reticulum cell sarcoma of hamsters.a transplantable reticulum cell sarcoma with leukemic manifestations can be transmitted from one hamster to another by means of a mosquito, aëdes aegypti (l.). the transmission seems to be by a transfer of tumor cells, and not by passage of some other oncogenic agent.196514280009
the genetics of black pedicel, a new colour mutant in aedes aegypti (l.). 196514297357
the influence of the gene fm (filarial susceptibility, brugia malayi) on the susceptibility of aedes aegypti to seven strains of brugia, wuchereria and dirofilaria. 196514297358
cross-over values in the sex chromosomes of the mosquito aedes aegypti and evidence of the presence of inversions. 196514297359
the effects of age and feeding on the susceptibility to insecticides of adult female, anopheles gambiae, aedes aegypti and culez pipiens fatigans. 196514297361
emergence and fertility of aedes aegypti (l.) reared in various concentrations of p32. 196514314951
dehydrochlorination and ddt-resistance in culex mosquitos.the mechanism of resistance to ddt was investigated in culex mosquitos to ascertain whether it was associated with detoxication to dde as in houseflies and aedes aegypti. resistant larvae of c. tarsalis were found to effect this dehydrochlorination, whereas the susceptible larvae did not; resistant larvae of c. fatigans completely converted all the absorbed ddt to the metabolite dde. enzyme assays in vitro showed that the resistant c. fatigans had 10 times the dehydrochlorinating activity, and r ...014315723
a genetical study of ddt-resistance in the trinidad strain of aedes aegypti l.previous genetical investigations on the trinidad ddt-resistant strain of aedes aegypti, involving mass crosses with susceptible strains, had indicated that a single major semi-dominant gene controlled resistance to ddt. the present study, in which single-pair matings were made, indicates that the genetic control of resistance is, in fact, more complex. the considerable variation observed could not be interpreted in terms of normal segregation at a single locus. at least three genetic factors ap ...196514315724
physiological and genetical studies on resistance to ddt substitutes in aedes aegypti. 196514317630
studies on mosquitoes and their natural enemies in bangkok. 2. insecticide susceptibility of the larvae of culex pipiens fatigans, aedes aegypti and the mosquito-eating fish lebistes reticulatus. 196514326223
measurement of salivation by aedes aegypti (l.) feeding on a living host. 196514330766
histochemical observations on aedes aegypti infected with larvae of brugia malayi. 196514347467
toxicity of aromatic acids to the larvae of the mosquito aedes aegypti l. and the counteracting influence of amino acids. 014351182
a preliminary aedes aegypti survey. 195414355279
[infectibility of aedes aegypti from chicks infected with plasmodium gallinaceum in incubation stage]. 195414355424
the laboratory transmission of yellow fever by aëdes (stegomyia) aegypti (linnaeus) from malaya. 195514362420
[monthly report on the aedes aegypti control program in american countries and territories]. 195414363372
[monthly report on the campaign of eradication of aedes aegypti in the countries and territories of the americas]. 195514389431
viruses associated with epidemic hemorrhagic fevers of the philippines and thailand.epidemiologic, clinical, and etiologic studies were carried out on a newly recognized, frequently fatal, pediatric disease syndrome which occurred in urban areas infested with aedes aegypti mosquitoes. four types of dengue virus (two of which are new), chikungunya virus, and another virus yet to be identified were isolated from the blood of patients. dengue viruses, types 2 and 3, were isolated from the mosquitoes. ample serologic confirmation was obtained of concurrent hemorrhagic fever and inf ...196014399343
genetic studies of aedes aegypti. 196114447858
the intake of brugia malayi by different strains of aedes aegypti. 196114455352
multiplication of vesicular stomatitis virus in aedes aegypti (l.) mosquitoes. 196214477586
observations on the development of setaria labiatopapillosa using new techniques for infecting aedes aegypti with this nematode. 196214478913
artificial infection of aedes aegypti with semliki forest virus by intrahaemocoelic injection. 196114480780
the development of brugia malayi in aedes aegypti. 196114490050
novel fermentation media for production of bacillus thuringiensis subsp. israelensis.the production of bacillus thuringiensis subsp. israelensis (debarjac) (bti) as a biopesticide is not cost-effective using existing fermentation technology. in this study, we explored the use of several less expensive alternative culture media (potato, common sugar, and bengal gram) for the growth and production of bti. growth was obtained in all tested media and was comparable to that obtained in conventional medium (luria-bertani). toxicity assays showed that the toxin produced from the novel ...200314503573
gene vector and transposable element behavior in mosquitoes.the development of efficient germ-line transformation technologies for mosquitoes has increased the ability of entomologists to find, isolate and analyze genes. the utility of the currently available systems will be determined by a number of factors including the behavior of the gene vectors during the initial integration event and their behavior after chromosomal integration. post-integration behavior will determine whether the transposable elements being employed currently as primary gene vect ...200314506218
transport mechanisms of diuresis in malpighian tubules of insects.we have studied malpighian tubules of aedes aegypti using a variety of methods: ramsay fluid secretion assay, electron probe analysis of secreted fluid, in vitro microperfusion and two-electrode voltage clamp. collectively, these methods have allowed us to elucidate transepithelial transport mechanisms under control conditions and in the presence of diuretic peptides. mosquito natriuretic peptide (mnp), a corticotropin-releasing factor (crf)-like diuretic peptide, selectively increases transepit ...200314506220
repellency and efficacy of a 65% permethrin spot-on formulation for dogs against aedes aegypti (diptera: culicidae) mosquitoes.a topically applied 65% permethrin spot-on (defend exspot treatment for dogs, schering-plough animal health) used for flea and tick control on dogs was evaluated for repellency and efficacy against the yellow fever mosquito, aedes aegypti, a vector of canine filariasis. six dogs were randomly assigned to receive a single application of 65% permethrin on day 0 (n=3) or to remain untreated as controls (n=3). dogs were anesthetized and exposed to 100 unfed, female mosquitoes in screened cages for 2 ...200314506589
isolation and serotyping of dengue viruses by mosquito inoculation technique from clinically suspected cases of dengue fever.dengue virus was isolated from 44 clinically suspected cases of dengue fever by mosquito inoculation technique using aedes aegypti mosquito in the department of virology, bsmmu in the year 2000. identification of dengue virus was done by direct fluorescent antibody technique using fitc conjugated anti-dengue monoclonal antibody and serotyping was carried out by indirect fluorescent antibody technique using serotype specific monoclonal anti-dengue antibody. the dengue virus isolation rate was 41. ...200214509382
enduring toxicity of transgenic anabaena pcc 7120 expressing mosquito larvicidal genes from bacillus thuringiensis ssp. israelensis.persistence of biological control agents against mosquito larvae was tested under simulated field conditions. mosquito larvicidal activity of transgenic anabaena pcc 7120 expressing cry4aa, cry11aa and p20 from bacillus thuringiensis ssp. israelensis was greater than b. thuringiensis ssp. israelensis primary powder (fun 89c06d) or wettable powder (wp) (bactimos products) when either mixed with silt or exposed to sunlight outdoors. reduction of bactimos primary powder toxicity was at least 10-fol ...200314510853
the peritrophic matrix limits the rate of digestion in adult anopheles stephensi and aedes aegypti mosquitoes.the peritrophic matrix (pm) is a chitin-containing acellular sheath that surrounds the blood meal and separates the food bolus from the midgut epithelium. intense molecular traffic through the pm occurs during digestion. digestive enzymes secreted by the midgut epithelium must traverse the pm to reach their substrates in the food bolus, and digestion products must cross the pm in the opposite direction to be absorbed by the epithelial cells. here we report that the pm limits the rate of digestio ...200314511821
junonia coenia densovirus-based vectors for stable transgene expression in sf9 cells: influence of the densovirus sequences on genomic integration.the invertebrate parvovirus junonia coenia densovirus (jcdnv) shares similarities with terminal hairpins and nonstructural (ns) protein activities of adeno-associated virus (aav) despite their evolutionary divergence (b. dumas, m. jourdan, a. m. pascaud, and m. bergoin, virology, 191:202-222, 1992, and c. ding, m. urabe, m. bergoin, and r. m. kotin, j. virol. 76:338-345, 2002). we demonstrate here that persistent transgene expression in insect cells results from stable integration of transfected ...200314512554
a targeted approach to the identification of candidate genes determining susceptibility to plasmodium gallinaceum in aedes aegypti.the malaria parasite, plasmodium, has evolved an intricate life cycle that includes stages specific to a mosquito vector and to the vertebrate host. the mosquito midgut represents the first barrier plasmodium parasites encounter following their ingestion with a blood meal from an infected vertebrate. elucidation of the molecular interaction between the parasite and the mosquito could help identify novel approaches to preventing parasite development and subsequent transmission to vertebrates. we ...200314513362
following in soper's footsteps: northeast brazil 63 years after eradication of anopheles gambiae.sub-saharan africa has long suffered under the yoke of the anopheles gambiae mosquito, but for northeast brazil (figure 1) its arrival over 60 years ago was a new and horrifying experience. this african mosquito is an exceptionally effective malaria vector because it is well adapted to feeding upon people and to exploiting aquatic habitats associated with our daily activities. anopheles gambiae sensu lato probably accounts for most of the world's malaria deaths and socioeconomic burden. fortunat ...200314522266
surveillance for the dengue vector aedes aegypti in tobago, west indies.an island-wide house survey was conducted in january 2002 to determine the geographic distribution, container profile, and population density of the aedes aegypti in tobago, west indies. the results showed the ae. aegypti infestation levels were significantly different (p > 0.01) among the 4 districts, with greater infestation levels (p > 0.01) observed in the northern and windward districts than in the central and leeward districts. from the 50 towns in tobago, houses were found positive in del ...200314524540
quantitative evaluation of funnel traps for sampling immature aedes aegypti from water storage jars.the trend is increasing to incorporate assessments of abundance into surveys for immature aedes aegypti to identify the most important types of containers that should be targeted for control. in this study, we examined whether funnel traps could be used to sample immature ae. aegypti from water storage jars ranging in size from 0.28-m diameter (30 liters) to 0.52-m diameter (150 liters). the effects of jar size and duration of funnel trap sampling were investigated and a set of calibration facto ...200314524543
an adulticidal sticky ovitrap for sampling container-breeding mosquitoes.the efficacy of a standard ovitrap and an ovitrap featuring an internal wall covered by a polybutylene adhesive was compared in field studies in cairns, australia. the sticky ovitrap was as effective as the standard ovitrap in detecting aedes aegypti, with 67.5% and 64% of traps positive for ae. aegypti, respectively. significantly higher numbers of ae. aegypti were collected by traps set outside rather than inside premises. sticky ovitraps also readily collected ochlerotatus notoscriptus and, e ...200314524545
laboratory evaluation of toxicity of 16 insect repellents in aerosol sprays to adult mosquitoes.sixteen commercial insect repellents (6 botanical and 10 synthetic organic products) in spray formulations were evaluated in the laboratory for adult knockdown (kd) and mortality of laboratory-reared female aedes aegypti, aedes albopictus, and anopheles quadrimaculatus. all tested products produced significant posttreatment kd and 24 h mortality of all 3 mosquito species. in our study, the synthetic organic repellents induced faster kd and kd of higher magnitude in adult mosquitoes than the bota ...200314524551
comparative toxicity of permethrin- and bifenthrin-treated cloth fabric for anopheles farauti and aedes aegypti.in this laboratory study, we applied 3 formulations of permethrin (peregin 500 ec, dragnet 500 ec, and dragnet 100 me) and 2 of bifenthrin (biflex 10 me and talstar 80 sc) to swatches of australian military shirt fabric. the knockdown and mortality of anopheles farauti and aedes aegypti after exposure to treated fabrics were compared. the mortality of an. farauti exposed to permethrin-treated swatches for 3 min in world health organization test kits was 94.2-100% after initial treatment and fell ...200314524552
mosquito vector control and biology in latin america--an eleventh symposium.the 11th annual latin american symposium presented by the american mosquito control association (amca) was held as part of the 67th annual meeting in dallas, tx, in february 2001. the principal objective, as for the previous 10 symposia, was to promote participation in the amca by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 45 presentations that were given orally in spanish or presented as posters by participants ...200114529084
laboratory and field evaluations of the insect repellent 3535 (ethyl butylacetylaminopropionate) and deet against mosquito vectors in thailand.the insect repellents 3535 (ethyl butylacetylaminopropionate or ir3535) and deet (n,n-diethyl-3-methylbenzamide) were prepared as 20% solutions in absolute ethanol and evaluated for repellency against many mosquito species in thailand under laboratory and field conditions using human subjects. in the laboratory, 0.1 ml was applied per 30-cm2 of exposed area on a volunteer's forearm (0.66-0.67 mg active ingredient [ai]/ cm2), whereas in the field, volunteers' legs (from knee to ankle, with a surf ...200114529087
identification of two isoforms of aminopeptidase n in aedes aegypti larval midgut.the bacterium bacillus thuringiensis produces toxin inclusions that are deleterious to target insect larvae. these toxins are believed to interact with a specific receptor protein(s) that is present on the gut epithelial cells of the larvae. in various insect species (in particular those belonging to the lepidopteran class), aminopeptidase n (apn) is one of the two receptor proteins that are considered to be involved in toxin-receptor interactions. however, in mosquitoes, the nature and identity ...200314536036
culex pipiens pipiens: characterization of immune peptides and the influence of immune activation on development of wuchereria bancrofti.stimulating or augmenting the innate immune response of insect vectors has been shown to impede or disrupt the development and transmission of eukaryotic pathogens; however, the majority of such studies have utilized model systems and not natural parasite-vector systems. the culex pipiens complex of mosquitoes functions as a primary urban vector of wuchereria bancrofti, a causative agent of lymphatic filariasis. to test the effects of immune activation on this vector-parasite interaction, culex ...200314550895
dengue 2 pdk-53 virus as a chimeric carrier for tetravalent dengue vaccine development.attenuation markers of the candidate dengue 2 (d2) pdk-53 vaccine virus are encoded by mutations that reside outside of the structural gene region of the genome. we engineered nine dengue virus chimeras containing the premembrane (prm) and envelope (e) genes of wild-type d1 16007, d3 16562, or d4 1036 virus within the genetic backgrounds of wild-type d2 16681 virus and the two genetic variants (pdk53-e and pdk53-v) of the d2 pdk-53 vaccine virus. expression of the heterologous prm-e genes in the ...200314557629
effect of rotenoids from the seeds of millettia dura on larvae of aedes aegypti.a crude chloroform extract of seeds of millettia dura dunn (leguminosae) showed high activity (lc50 = 3.5 microg ml(-1) at 24 h) against second-instar larvae of the mosquito, aedes aegypti l (diptera: culicidae). the rotenoids, deguelin and tephrosin, isolated from the seeds of this plant also showed potent activities, with lc50 values of 1.6 and 1.4 microg ml(-1) at 24 h, respectively. the related rotenoids millettone and millettosin were inactive at 20 microg ml(-1). saturation at the b/c ring ...200314561074
role of gregarine parasite ascogregarina culicis (apicomplexa: lecudinidae) in the maintenance of chikungunya virus in vector mosquito.ascogregarina culicis and ascogregarina taiwanensis are common gregarine parasites of aedes aegypti and aedes albopictus mosquitoes, respectively. these mosquito species are also known to transmit dengue and chikungunya viruses. the sporozoites of these parasites invade the midgut epithelial cells and develop intracellularly and extracellularly in the gut to complete their life cycles. the midgut is also the primary site for virus replication in the vector mosquitoes. therefore, studies were car ...200314563178
blood meal induces global changes in midgut gene expression in the disease vector, aedes aegypti.blood feeding is an essential developmental process for many arthropods and plays a significant role in disease transmission. understanding physiological responses in the midgut is important because it is the primary site of blood meal digestion and pathogenic infection. processes that occur in the midgut in response to a blood meal have been studied but are poorly understood. here, we use cdna microarrays to examine midgut gene expression on a global level in response to blood feeding to assist ...200314563362
infection of malaria (anopheles gambiae s.s.) and filariasis (culex quinquefasciatus) vectors with the entomopathogenic fungus metarhizium anisopliae.current intra-domiciliary vector control depends on the application of residual insecticides and/or repellents. although biological control agents have been developed against aquatic mosquito stages, none are available for adults. following successful use of an entomopathogenic fungus against tsetse flies (diptera: glossinidae) we investigated the potency of this fungus as a biological control agent for adult malaria and filariasis vector mosquitoes.200314565851
1,5-disubstituted imidazoles inhibit juvenile hormone biosynthesis by the corpora allata of the mosquito aedes aegypti.we investigated the effect of fifteen 1,5-disubstituted imidazoles (1,5-dis) on juvenile hormone iii (jh iii) and methyl farnesoate (mf) biosynthesis by the corpora allata (ca) of the mosquito aedes aegypti in vitro. four compounds (th-35, th-83, th-62 and th-28) significantly decreased jh biosynthesis in the ca dissected from 3-day old sugar-fed females. the decrease of jh synthesis was not always associated with increased mf. th-30 and th-83 increased mf levels, while th-85 and th-61 significa ...200314568578
microbial ecology of bacillus thuringiensis: fecal populations recovered from wildlife in korea.a total of 34 fecal samples, collected from 14 species of wild mammals in korea, were examined for the occurrence of bacillus thuringiensis. the organism was detected in 18 (53%) samples. among the three food-habit groups, herbivorous animals yielded the highest frequency (69%) of samples positive for b. thuringiensis, followed by omnivorous animals (50%). of the six fecal samples from carnivorous animals, only one sample contained b. thurin giensis. among 527 isolates belonging to the bacillus ...200314569287
[scanning electron microscopy of eggs of haemagogus leucocelaenus (diptera: culicidae)].to observe morphological details of the eggs of haemagogus (conopostegus) leucocelaenus, seen for the first time via scanning electron microscopy (sem), with morphometric analysis of the main structures.200314569344
[household water reservoirs and control of aedes aegypti].water reservoirs for domestic use are important sites for the development of aedes aegypti. in poor areas, these reservoirs are often located outdoors upon flat rooftops. in modern architecture buildings, however, the same is seen even in high-class condominiums. special attention should be given to these modern architectural constructions during entomological surveillance.200314569348
a family of genes clustered at the triplo-lethal locus of drosophila melanogaster has an unusual evolutionary history and significant synteny with anopheles gambiae.within the unique triplo-lethal region (tpl) of the drosophila melanogaster genome we have found a cluster of 20 genes encoding a novel family of proteins. this family is also present in the anopheles gambiae genome and displays remarkable synteny and sequence conservation with the drosophila cluster. the family is also present in the sequenced genome of d. pseudoobscura, and homologs have been found in aedes aegypti mosquitoes and in four other insect orders, but it is not present in the sequen ...200314573474
effects of a brassinosteroid analogue to mosquito larvae.many plant compounds are able to modulate herbivore growth and reproduction by directly interacting with steroid hormones systems. in insects, several classes of phytochemicals, including brassinosteroids and related substances, interfere with molting and reproduction. the effects of the molting-hormone antagonist pesticide di-31, a brassinosteroid analogue, on aedes aegypti mosquito larvae were tested in two different exposure scenarios. after static exposure of first-instar larvae, the calcula ...200314575682
[centenary of the discovery of yellow fever virus and its transmission by a mosquito (cuba 1900-1901)].during the 17th, 18th and 19th centuries, yellow fever has been an ordinary scourge in american cities. in the early part of the 20th century, a main discovery has been achieved in havana city, cuba, leading to direct consequences for tropical medicine, public health and virology. there, the u.s. army yellow fever commission headed by walter reed proved that yellow fever was a viral disease transmitted by a specific mosquito, stegomyia fasciata, in fact aedes aegypti l. the idea that vomito negr ...200314582304
mosquito larvicidal activity of transgenic anabaena pcc 7120 expressing toxin genes from bacillus thuringiensis subsp. israelensis.genes encoding the mosquito larvicidal toxins cry4aa, cry11aa, cyt1aa and the regulatory p20 from bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing, filamentous cyanobacterium anabaena pcc 7120 for expression under control of two strong promoters p(psba) and p(a1). the clone prve4-adrc displayed toxicity against fourth-instar larvae of aedes aegypti, the highest ever achieved in cyanobacteria. it was about 2.5-fold more toxic than the respective clone without cyt ...200314592708
posttranscriptional control of the competence factor betaftz-f1 by juvenile hormone in the mosquito aedes aegypti.in anautogenous mosquitoes, vitellogenesis, which includes production of yolk protein precursors, requires blood feeding. consequently, mosquitoes transmit many diseases. understanding the molecular mechanisms of vitellogenesis regulation will contribute significantly to vector control strategies. newly emerged aedes aegypti females require 3 days before becoming competent to activate vitellogenesis in response to a blood-meal-initiated, elevated titer of 20-hydroxyecdysone (20e). an orphan nucl ...200314593204
rna interference-mediated knockdown of a gata factor reveals a link to anautogeny in the mosquito aedes aegypti.blood feeding tightly regulates the reproductive cycle in anautogenous mosquitoes. vitellogenesis (the synthesis of yolk protein precursors) is a key event in the mosquito reproductive cycle and is activated in response to a blood meal. before blood feeding, aedes aegypti is in a state of reproductive arrest during which the yolk protein precursor genes (ypps) are repressed. the regulatory region of the major ypp gene vitellogenin (vg) has multiple gata-binding sites required for the high expres ...200314595016
synergistic attraction of aedes aegypti (l.) to binary blends of l-lactic acid and acetone, dichloromethane, or dimethyl disulfide.kairomones produced by humans provide female anthropophilic mosquitoes with vital cues used in host-seeking for a blood meal. these chemicals are emanated primarily by the skin and provide the mosquitoes a means to orient themselves to humans at a relatively close range. chemical studies of these emanations have provided new ideas for the formulation of attractant blends. we report mosquito attraction responses for three binary blends and their separate components. the blends are comprised of l- ...200314596278
activity of the corpora allata of adult female aedes aegypti: effects of mating and feeding.the synthesis of juvenile hormone iii (jh iii) by the isolated corpora allata (ca) of aedes aegypti adult female was studied using an in vitro radiochemical assay. we dissected the corpora allata-corpora cardiaca (ca-cc) complex attached to a piece of aorta. the complex was left connected to the intact head capsule to facilitate the visualization and transfer of the glands. a linear increase in the cumulative amount of biosynthesized jh iii was found for at least the first 6 h of incubation; app ...200314599502
high efficiency, site-specific excision of a marker gene by the phage p1 cre-loxp system in the yellow fever mosquito, aedes aegypti.the excision of specific dna sequences from integrated transgenes in insects permits the dissection in situ of structural elements that may be important in controlling gene expression. furthermore, manipulation of potential control elements in the context of a single integration site mitigates against insertion site influences of the surrounding genome. the cre-loxp site-specific recombination system has been used successfully to remove a marker gene from transgenic yellow fever mosquitoes, aede ...200314602940
diagnosis of dengue virus infection by the visual and simple aubiodot immunoglobulin m capture system.the dengue igm capture elisa (mac-elisa) is the immunoenzymatic system recommended by the pan american health organization and the world health organization for the serological diagnosis of dengue virus infection due to its high sensitivity, ease of performance, and use of a single acute-phase serum sample. however, tests with this enzyme-linked immunosorbent assay (elisa) system are time-consuming and require equipment for washing, incubation, and reading of the results. aubiodot is a multistep ...200314607869
[application of one step rt-pcr assay for detection of flavivirus rna in mosquitoes].the conditions of one step rt-pcr method for detection of virus rna in field-collected mosquitoes, and preservation period of infected mosquitoes for one step rt-pcr were examined. we compared several virus rna extraction methods with artificially contaminated mosquito pools with dengue virus (dv), japanese encephalitis virus (jev), and yellow fever virus (yfv) with a known amount of plaque forming unit (pfu) to establish the condition of one step rt-pcr. in this study, most effective rna extrac ...200314608915
the non-ltr retrotransposons in ciona intestinalis: new insights into the evolution of chordate genomes.non-long terminal repeat (non-ltr) retrotransposons have contributed to shaping the structure and function of genomes. in silico and experimental approaches have been used to identify the non-ltr elements of the urochordate ciona intestinalis. knowledge of the types and abundance of non-ltr elements in urochordates is a key step in understanding their contribution to the structure and function of vertebrate genomes.200314611659
inhibition of interferon signaling by dengue virus.dengue virus is a worldwide-distributed mosquito-borne flavivirus with a positive strand rna genome. its transcribed polyprotein is cleaved by host- and virus-encoded peptidases into 10 proteins, some of which are of unknown function. although dengue virus-infected cells seem to be resistant to the antiviral action of ifn, the viral products that mediate this resistance are unknown. therefore, we have analyzed the ability of the 10 dengue virus-encoded proteins to antagonize the ifn response. we ...200314612562
Displaying items 3101 - 3200 of 14974