Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| pamam-lys, a novel vaccine delivery vector, enhances the protective effects of the sjc23 dna vaccine against schistosoma japonicum infection. | schistosomiasis japonica remains a major public-health concern in china. praziquantel-based chemotherapy effectively reduces both infections and intensity; however, it can not prevent re-infection. furthermore, there is an increasing concern about praziquantel resistance following long-term repeated use of the drug in endemic areas. therefore, development of a schistosomiasis vaccine, as a strategy to prevent and control schistosomiasis japonica, has been given high priority. the present study w ... | 2014 | 24497955 |
| molecular differentiation of schistosoma japonicum and schistosoma mekongi by real-time pcr with high resolution melting analysis. | human schistosomiasis caused by schistosoma japonicum and schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of asia. due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. high resolution melting (hrm) real-time pcr is developed as an alternative tool for the detection and differentiation of these 2 species. a primer pair was designed for targeting the 18s ribosomal rna gene to ... | 2013 | 24516269 |
| assessment of morbidity due to schistosoma japonicum infection in china. | this paper presents a historical assessment of morbidity due to the schistosoma japonicum infection in china. due to the socio-economic situation, which did not allow for a control program to be implemented until the early 1950s, morbidity was serious and mortality was high before this. based on a few investigations and published papers, it can be said that the disease caused millions of deaths, and destroyed numerous families and villages. since the 1950s, there has been a national control prog ... | 2014 | 24529186 |
| molecular characterization and serological reactivity of a vacuolar atp synthase subunit ε-like protein from clonorchis sinensis. | the vacuolar atpase enzyme complex (v-atpase) pumps protons across membranes, energized by hydrolysis of atp. extensive investigations on structural and biochemical features of these molecules have implied their importance in the physiological process. in this study, a full-length sequence encoding a vacuolar atp synthase subunit ε-like protein of clonorchis sinensis (csatp-ε) was isolated from our cdna library. the hypothetical 226 amino acid sequence shared 76% identity with atp-ε proteins of ... | 2014 | 24535733 |
| schistosoma japonicum egg specific protein sje16.7 recruits neutrophils and induces inflammatory hepatic granuloma initiation. | neutrophils are known to play a major role in the egg granulomatous lesions caused by schistosoma japonicum, but the precise mechanism by which eggs recruit or active neutrophil is unknown. here we report s. japonicum egg specific ef-hand protein-sje16.7 is a potent neutrophil recruiter and initiates the egg associated inflammatory granuloma in schistosomiasis. we show that the expression of sje16.7 at level of both mrna and protein is restricted to the egg stage. it locates in the miracidium an ... | 2014 | 24551263 |
| monitoring the transmission of schistosoma japonicum in potential risk regions of china, 2008 - 2012. | schistosomiasis japonica, caused by schistosoma japonicum infection, remains a major public health concern in china, and the geographical distribution of this neglected tropical disease is limited to regions where oncomelania hupensis, the intermediate host of the causative parasite, is detected. the purpose of this study was to monitor the transmission of s. japonicum in potential risk regions of china during the period from 2008 through 2012. to monitor the transmission, 10 fixed surveillance ... | 2014 | 24566053 |
| new integrated strategy emphasizing infection source control to curb schistosomiasis japonica in a marshland area of hubei province, china: findings from an eight-year longitudinal survey. | schistosomiasis remains a major public health problem in china. the major endemic foci are the lake and marshland regions of southern china, particularly the regions along the middle and lower reach of the yangtze river in four provinces (hubei, hunan, jiangxi, and anhui). the purpose of our study is to assess the effect of a new integrated strategy emphasizing infection source control to curb schistosomiasis in marshland regions. | 2014 | 24587030 |
| immune responses result in misdiagnosis of schistosoma japonicum by immunodiagnosis kits in egg-positive patients living in a low schistosomiasis transmission area of china. | in recent field surveys, we failed to detect the presence of specific antibody against schistosoma japonicum in some egg-positive patients by commonly used immunodiagnostic kits. to find out whether low levels of specific antibody truly exist among egg-positive individuals and elucidate the underlying immune mechanisms, we carried out a cross-sectional epidemiologic study in a s. japonicum low transmission endemic area of poyang lake region, china and compared the humoral and cellular immune cha ... | 2014 | 24598061 |
| sensitivity of oncomelania hupensis to niclosamide: a nation-wide survey in china. | schistosomiasis japonica, transmitted by the intermediate host snail oncomelania hupensis of the causative agent schistosoma japonicum, remains a major public-health concern in china, and control of this snail is one of the major approaches used in attempts to interrupt the transmission of this neglected tropical disease. niclosamide is currently the only commercial molluscicide available for the control of o. hupensis snails in china. the purpose of this study was to evaluate the current sensit ... | 2014 | 24625624 |
| inhibitory effects and analysis of rna interference on thioredoxin glutathione reductase expression in schistosoma japonicum. | schistosomes infect around 280 million people worldwide. the worms survive in the veins of the final host, where thioredoxin glutathione reductase (tgr) activity helps the parasites to survive in the aerobic environment. in the present study, we synthesized 4 small interfering rnas (sirna s1, s2, s3, and s4) targeting the schistosoma japonicum (sj) tgr gene and used them to knockdown the tgr gene. the knockdown effects of the sirnas on sjtgr, and the thioredoxin reductase (trxr) activity of sjtg ... | 2014 | 24628421 |
| cpg oligodeoxynucleotide ligand potentiates the activity of the pvax1-sj26gst. | schistosomiasis is considered one of the most important neglected tropical diseases and remains a major public health problem in endemic countries. toll-like receptor (tlr) ligands have been investigated as potential vaccine adjuvants for tumor and virus immunotherapy. however, few tlr ligands affecting schistosoma vaccines have been characterized. in this study, we evaluated a tlr9 ligand (cpg oligodeoxynucleotide 1826, cpg) as an adjuvant for a partially protective dna vaccine encoding a 26-kd ... | 2013 | 24648995 |
| serodiagnosis of schistosoma japonicum infection: genome-wide identification of a protein marker, and assessment of its diagnostic validity in a field study in china. | schistosomiasis remains a highly prevalent and serious parasitic disease. a major factor preventing its effective management is the scarcity of effective diagnostic tools. we did a genome-wide identification of diagnostic protein markers for schistosome infection and assessed their diagnostic validity in a field study. | 2014 | 24656567 |
| parasitic antigens alter macrophage polarization during schistosoma japonicum infection in mice. | schistosome eggs are trapped in host liver and elicit severe hepatic granulomatous inflammation, which can lead to periportal fibrosis, portal hypertension, hemorrhage, or even death in the host. it was reported that the macrophage plays an important role in host immune responses to schistosome infection. nitric oxide (no) produced by classically activated macrophages (m1 macrophages) is cytotoxic to schistosomula and can prevent hepatic schistosomal fibrosis, while arginase-1 (arg-1) expressed ... | 2014 | 24666892 |
| parasite densities modulate susceptibility of mice to cerebral malaria during co-infection with schistosoma japonicum and plasmodium berghei. | malaria and schistosomiasis are endemic and co-exist in the same geographic areas, even co-infecting the same host. previous studies have reported that concomitant infection with schistosoma japonicum could offer protection against experimental cerebral malaria (ecm) in mice. this study was performed to evaluate whether alterations in parasite density could alter this protective effect. | 2014 | 24670210 |
| field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of china. | schistosomiasis remains a serious public health problem in affected countries, and routine, highly sensitive and cost-effective diagnostic methods are lacking. we evaluated two immunodiagnostic techniques for the detection of schistosoma japonicum infections: circulating antibody and circulating antigen assays. | 2014 | 24684924 |
| expression of microrna-454 in tgf-β1-stimulated hepatic stellate cells and in mouse livers infected with schistosoma japonicum. | in the process of hepatic fibrosis, hepatic stellate cells (hscs) can be activated by many inflammatory cytokines. the transforming growth factor-β1 (tgf-β1) is one of the main profibrogenic mediators. recently, some studies have also shown that micrornas (mirnas) play essential roles in the progress of liver fibrosis by being involved in the differentiation, fat metabolism and ecm production of hscs. | 2014 | 24685242 |
| genetic structure inferred from mitochondrial 12s ribosomal rna sequence of oncomelania quadrasi, the intermediate snail host of schistosoma japonicum in the philippines. | species and subspecies of the oncomelania hupensis species complex are recognized as intermediate hosts of schistosoma japonicum. of these species and subspecies, o. quadrasi is distributed throughout the philippines. this study used 12s ribosomal rna sequences to explore the genetic structure of o. quadrasi populations in the philippines. three subspecies, o. h. hupensis, o. h. formosana, and o. h. chiui of this group were also examined. the phylogenetic tree and haplotypes network showed that ... | 2014 | 24686739 |
| childhood malnutrition and parasitic helminth interactions. | there is evidence to support that nutritional deficiency can reduce the body's immune function, thereby decreasing resistance to disease and increasing susceptibility to intestinal parasites. | 2014 | 24704723 |
| novel expression profiles of micrornas suggest that specific mirnas regulate gene expression for the sexual maturation of female schistosoma japonicum after pairing. | schistosoma japonicum is one of the major causative agents of schistosomiasis. the pairing of males and females leads to female sexual maturation and maintains this mature state. however, the mechanisms by which pairing facilitates sexual maturation are yet to be investigated. | 2014 | 24721600 |
| the effect of recombinant stgfβ1rii and sil13rα2 receptor proteins on schistosomiasis japonica, hepatic fibrosis and signal transduction in a mouse model of schistosome disease. | this study was designed to investigate the effect of recombinant stgfβ1rii and sil13rα2 receptor proteins on schistosomiasis japonica, hepatic fibrosis and the expression of smad3 and stat6. the proteins stgfβ1rii and sil13rα2 were expressed in escherichiacoli, purified using affinity chromatography and characterized by western blotting. female balb/c mice (48) were randomly divided into eight groups and infected with schistosoma japonicum. five weeks after infection, test groups were injected w ... | 2014 | 24746639 |
| effects of microtus fortis lymphocytes on schistosoma japonicum in a bone marrow transplantation model. | microtus fortis is a non-permissive host for schistosoma japonicum. while m. fortis lymphocytes are known to provide natural resistance against s. japonicum, the specific mechanism remains unclear. a bone marrow transplantation (bmt) model was established using immunodeficient mice, either nude (experiment 1) or v(d)j recombination activation gene deficient mice (rag-1(-/-)) (experiment 2) as recipients and m. fortis or c57bl/6 mice as donors. the growth and development of s. japonicum were eval ... | 2014 | 24746640 |
| eco-social determinants of schistosoma japonicum infection supported by multi-level modelling in eryuan county, people's republic of china. | schistosomiasis remains of considerable public health concern in many tropical and subtropical regions of the world, including the people's republic of china (p.r. china). the effectiveness of schistosomiasis control interventions are, among other factors, governed by the social-ecological context. however, eco-social determinants of schistosomiasis are poorly understood, particularly at the household or village levels. in the current study, residents in 26 villages of eryuan county, yunnan prov ... | 2015 | 24751418 |
| discovery and confirmation of ligand binding specificities of the schistosoma japonicum polarity protein scribble. | schistosomiasis is a chronic debilitating parasitic disease that afflicts more than 200 million individuals worldwide. long-term administration of chemotherapy with the single available drug, praziquantel, has led to growing concerns about drug resistance. the psd-95/dlg/zo-1 (pdz) domain is an important module found in many scaffolding proteins, which has been recognized as promising targets for the development of novel drugs. however, the parasite-derived pdz domains and their associated funct ... | 2014 | 24784152 |
| follicular helper t cells promote liver pathology in mice during schistosoma japonicum infection. | following schistosoma japonicum (s. japonicum) infection, granulomatous responses are induced by parasite eggs trapped in host organs, particular in the liver, during the acute stage of disease. while excessive liver granulomatous responses can lead to more severe fibrosis and circulatory impairment in chronically infected host. however, the exact mechanism of hepatic granuloma formation has remained obscure. in this study, we for the first time showed that follicular helper t (tfh) cells are re ... | 2014 | 24788758 |
| [establishment of a1e3 and b1c4 monoclonal antibody-based elisa for detecting circulating antigen of schistosoma japonicum and its preliminary application]. | to establish a1e3 and b1c4 monoclonal antibody-based elisa for detecting circulating antigen of schistosoma japonicum and explore its application value in the field. | 2014 | 24800564 |
| [preliminary observation on the effect of mefloquine against egg granuloma formation in the liver of mice infected with schistosoma japonicum]. | to explore whether mefloquine possesses the effect on granuloma formation induced by schistosoma japonicum eggs. | 2013 | 24809183 |
| [expression of smad proteins in the process of liver fibrosis in mice infected with schistosoma japonicum]. | to study the expression of smads proteins involved in tgf-beta1 signal transduction during the process of liver fibrosis in balb/c mice infected with schistosoma japonicum. | 2013 | 24809185 |
| [effect of paeoniflorin on secretion of tgf-beta1 from macrophages in mice]. | to explore the effect of paeoniflorin (pae) on the production of transforming growth factor beta1 (tgf-beta1) from peritoneal macrophages(pms) stimulated by soluble egg antigen (sea) of schistosoma japonicum. | 2008 | 24812806 |
| [new polymorphic microsatellite loci identified using genomic resource for schistosoma japonicum]. | to identify new microsatellite loci from genome sequence database for the study of polymorphism of schistosoma japonicum. | 2008 | 24812811 |
| [studies on immunomodulation effect of recombinant sj16 from schistosoma japonicum on inflammation response of host]. | to study the immunomodulation effect of the recombination sj16 from schistosoma japonicum (resj16) on inflammation response of host. | 2008 | 24812813 |
| [construction and expression of recombinant baculovirus with schistosoma japonicum sjpp gene]. | to express the soluble recombinant schistosoma japonicum sjpp proteins in tn5b1-4 cells. | 2008 | 24812815 |
| [analysis of karyotype and preparation of c-band and g-band in schistosoma japonicum]. | chromosomes of schistosoma japonicum were prepared by usual air drying method, c-band and g-band were made by modified bsg method and enzyme digestion method respectively. results showed that the karyotype of s. japonicum was 4m + 6sm + 4st + 2sex chromosome and the c-band formula was 2n = 5cit + 4ci(+) + 3ci + 2ct +2ct. | 2008 | 24812821 |
| [polymorphism in the partial mitochondrial nad4 gene of schistosoma japonicum]. | the partial nadh dehydrogenase subunit 4 (pnad4) fragment was amplified by polymerase chain reaction (pcr) from schistosoma japonicum collected from 4 areas in hunan province, and the amplicons were cloned and sequenced. a 480 bp sequence was obtained and the nucleotide difference between samples from the four locations were 0.4%-1.3%. low level of sequence variability between and within different populations of s. japonicum was detected. the results are worthwhile for further study on the popul ... | 2008 | 24812822 |
| [improved preparation of pure alive eggs of schistosoma japonicum]. | to prepare a large amount of pure alive schistosoma japonicum eggs, rabbit was infected with 2000 cercariae and its liver was taken aseptically 38-45 days after infection and homogenized. the homogenate was screened through different sieves(60, 120, 200, 300, 360 meshes per inch respectively), and washed with 1.2% nacl. the eggs and leftover were then digested with 0.25% trypsin for 2 hours, sieved over 360 meshes per inch and washed with rpmi 1640 medium. the collected eggs reached to (95.1 +/- ... | 2008 | 24812826 |
| [screening and analysis of peptides specifically binding to the schistosomulum tegument of schistosoma japonicum]. | to screen and analyze the peptides in 12 phage-display peptide library specifically binding to the schistosomulum, not cercaria, tegument of schistosoma japonicum. | 2013 | 24812833 |
| [comparative observation on inhibition of hemozoin formation and their in vitro and in vivo anti-schistosome activity displayed by 7 antimalarial drugs]. | to observe and compare the inhibition of hemozoin formation and the in vitro as well as in vivo antischistosomal activity induced by seven antimalarial drugs. | 2013 | 24812850 |
| [eukaryotic expression of sje16, sjppiase and sjrobl genes from schistosoma japonicum egg and evaluation of their role in immunodiagnosis]. | to express schistosoma japonicum egg proteins by eukaryotic system and evaluate their role in schistosomiasis immunodiagnosis. | 2013 | 24812851 |
| [evaluation of the comprehensive schistosomiasis control measures with emphasis on infection source of replacing cattle with machine]. | to evaluate the effect of comprehensive measures with an emphasis on schistosomiasis infection source control by replacing cattle with machine. | 2013 | 24812859 |
| [genetic variations of the elastase gene among eight populations of schistosoma japonicum]. | to investigate genetic diversity in the elastase gene among eight schistosoma japonicum populations, and whether natural selection occur. | 2013 | 24812875 |
| [screening and evaluation of schistosoma japonicum sjribosomal_l18a protein and its b cell epitopes]. | to screen a ribosomal protein (sjribosomal_l18a) of schistosoma japonicum and predict its b cell epitopes, and evaluate the potential diagnostic value of the recombinant protein and the synthetic b cell epitopes. | 2013 | 24812876 |
| [dynamic changes in professional and non-professional antigen presenting cells in the spleen from mice infected with schistosoma japonicum]. | objective; to observe the changes in various dendritic cell (dc) subsets, macrophages, basophils, eosinophils and mast cells in mouse spleen before and after schistosoma japonicum infection induced th2 response. | 2013 | 24812882 |
| [influence factors of schistosoma japonicum infection among fishermen in eastern dongting lake region]. | to investigate schistosome infection among the professional fishermen in yueyang county, east dongting lake region and its influence factors. | 2013 | 24812884 |
| phosphagen kinase in schistosoma japonicum: ii. determination of amino acid residues essential for substrate catalysis using site-directed mutagenesis. | phosphagen kinases (pks) play major roles in the regulation of energy metabolism in animals. creatine kinase (ck) is the sole pk in vertebrates, whereas several pks are present in invertebrates. we previously identified a contiguous dimer taurocyamine kinase (tk) from the trematode schistosoma japonicum (sj), a causative agent of schistosomiasis. sjtk contiguous dimer is comprised of domain 1 (d1) and domain 2 (d2). in this study, we used sjtk contiguous dimer (sjtkd1d2) or truncated single-doma ... | 2014 | 24815317 |
| allograft inflammatory factor-1 alleviates liver disease of balb/c mice infected with schistosoma japonicum. | allograft inflammatory factor-1 (aif-1) plays an important role in various inflammatory conditions. our previous study demonstrated that aif-1 was over-expressed in the liver of balb/c mice infected with schistosoma japonicum and played significant role in the pathogenesis of schistosomiasis. the aim of this study was to focus on the effect of aif-1 treatment on liver fibrosis and necrosis of balb/c mice infected with s. japonicum. seventy-two balb/c mice were infected with cercariae of s. japon ... | 2014 | 24816816 |
| [proteomic analysis of adult schistosoma japonicum treated with praziquantel]. | to analyze differentially expressed proteins of pairing adult schistosoma japonicum treated with praziquantel so as to further explore the action mechanism of praziquantel (pzq). | 2008 | 24818365 |
| [study on the spatio-temporal distribution of seroprevalence of schistosoma japonicum]. | to analyze and compare the spatio-temporal structure and risk factors of county-level seroprevalence of schistosoma japonicum infection in lake and mountainous regions. | 2008 | 24818374 |
| [in vitro effect of 5-hydroxytryptamine on motility and length of schistosoma japonicum primary sporocysts]. | to study in vitro the effect of 5-hydroxytryptamine (5-ht) on the motility and length of primary sporocysts of schistosoma japonicum and select the optimal concentration and time of 5-ht. | 2008 | 24818375 |
| [fluorescent quantitative real-time pcr for detection of schistosoma japonicum]. | to establish a sensitive and specific fluorescent quantitative real-time pcr method for the detection of schistosoma japonicum. | 2008 | 24818376 |
| [detection of toxoplasma gondii dna by loop-mediated isothermal amplification]. | to detect toxoplasma gondii dna by loop-mediated isothermal amplification (lamp). | 2008 | 24818377 |
| [effect of immune response mediated by icos signaling pathway on hepatic fibrosis in mice infected with schistosoma japonicum]. | to investigate the effect of th2 polarization mediated by icos signaling pathway on hepatic fibrosis in mice infected with schistosoma japonicum. | 2013 | 24818383 |
| [therapeutic effect of resveratrol as well as resveratrol combined with praziquantel on the liver fibrosis due to schistosoma japonicum infection in mice]. | to study the therapeutical effect and mechanisms of resveratrol and its combination with praziquantel on the liver fibrosis due to schistosoma japonicum infection. | 2013 | 24818384 |
| ultrastructural alterations of adult schistosoma japonicum harbored in mice treated with a single oral dose of oz78. | to observe the ultrastructural alterations of adult schistosoma japonicum induced by synthetic trioxolane oz78. | 2013 | 24818405 |
| [surveilance and response for schistosomiasis japonica based on sentinel mice examination for cercariae-infested water in risk region, 2012]. | to monitor water body infestation in schistosomiasis high-risk areas with sentinel mouse technique. | 2013 | 24818406 |
| [inhibition of culture supernatant of mesenchymal stem cells on macrophages raw264.7 activated by soluble egg antigen of schistosoma japonicum]. | to observe the inhibitive effect of rat mesenchymal stem cells (msc) culture supernatant on macrophages activated by soluble egg antigen (sea) of schistosoma japonicum. | 2011 | 24822341 |
| [effect of n-acetylcysteine on malondialdehyde and superoxide dismutase in hepatic tissue of mice with schistosomiasis japonica]. | 90 mice were randomly divided into six groups: normal control, infected control, long-term drug use group 1 (l1), long-term drug use group 2 (l2), short-term drug use group 1 (s1) and short-term drug use group 2 (s2). mice in all groups except those in the normal control group were infected with 30 cercariae of schistosoma japonicum through abdominal skin. n-acetylcysteine (nac) solution was orally given to mice in l1 and l2 groups, 200 mg/kg and 400 mg/kg, respectively, 2 times/d from the day o ... | 2011 | 24822348 |
| [accumulation of myeloid-derived suppressor cells in the spleen and peripheral blood of schistosoma japonicum-infected mice]. | to determine the accumulation of cd11b+ gr-1+ myeloid-derived suppressor cells (mdsc) in schistosorna japonicum-infected mice. | 2014 | 24822356 |
| [effect of schistosoma japonicum infection on serum lipid status in mice]. | to investigate the effect of schistosoma japonicum infection on lipid status in mouse serum. | 2014 | 24822357 |
| [effect of rna interference targeting schistosoma japonicum aldose reductase gene]. | to investigate the effect of sirna-induced rna interference (rnai) at both messenger rna (mrna) and protein level, targeting the schistosoma japonicum aldose reductase (sjar) gene. | 2014 | 24822358 |
| evaluation of recombinant sjlap and sjfbpa in detecting antibodies to schistosoma japonicum. | to investigate the early response of immunoglobulin g (igg) antibody responses to schistosoma japonicum infection in mice by using the recombinant proteins, s. japonicum leucine aminopeptidase (rsjlap) and s. japonicum fructose-1, 6-bisphosphate aldolase (rsjfbpa), and evaluate the potential of rsjlap and rsjfbpa in diagnosis as well as in assessment of therapeutic efficacy in human schistosomiasis. | 2011 | 24830194 |
| detection of active schistosome infection by cell-free circulating dna of schistosoma japonicum in highly endemic areas in sorsogon province, the philippines. | the current status of schistosomiasis in highly endemic areas is difficult to determine by ovum detection because of the superficially low parasite load after mass drug administration, whereas the parasite transmission rates are still high. cell-free parasite dna is fragments of parasite-derived dna existing in the host's body fluids. we conducted population-based studies to test the presence of cell-free schistosome dna in endemic areas of sorsogon province, the philippines. schistosome dna in ... | 2015 | 24836919 |
| de novo assembly and transcriptome characterization: novel insights into the natural resistance mechanisms of microtus fortis against schistosoma japonicum. | microtus fortis is a non-permissive host of schistosoma japonicum. it has natural resistance against schistosomes, although the precise resistance mechanisms remain unclear. the paucity of genetic information for m. fortis limits the use of available immunological methods. thus, studies based on high-throughput sequencing technologies are required to obtain information about resistance mechanisms against s. japonicum. | 2014 | 24886088 |
| study of the variation of schistosomiasis risk in lake poyang in the people's republic of china using multiple space-borne sensors for the monitoring and modelling. | the dynamics of the poyang lake in jiangxi province, people's republic of china has been monitored to demonstrate the association of various variables with the distribution of schistosomiasis transmission with particular reference to the annual variation of the habitats for the oncomelania snail, the intermediate host of schistosoma japonicum. this was studied with multiple space-borne sensors, including the envisat radar altimeter (ra-2) and modis/terra radiometry data products such as the 16-d ... | 2014 | 24893013 |
| defining a pro-inflammatory neutrophil phenotype in response to schistosome eggs. | neutrophils contribute to the pathological processes of a number of inflammatory disorders, including rheumatoid arthritis, sepsis and cystic fibrosis. neutrophils also play prominent roles in schistosomiasis japonica liver fibrosis, being central mediators of inflammation following granuloma formation. in this study, we investigated the interaction between schistosoma japonicum eggs and neutrophils, and the effect of eggs on the inflammatory phenotype of neutrophils. our results showed signific ... | 2014 | 24898449 |
| gene expression profile of lps-stimulated dendritic cells induced by a recombinant sj16 (rsj16) derived from schistosoma japonicum. | sj16, a 16-kda protein secreted from schistosoma japonicum, has been demonstrated an anti-inflammatory effect in vitro and in vivo, but its mechanism is still not clear. in this study, microarray analysis was performed to investigate the effects of recombinant sj16 (rsj16) on the gene expression of the lipopolysaccharide (lps)-stimulated dendritic cells (dcs). immature dcs were treated with lps, lps + recombinant sj16 (rsj16), or rsj16 alone for 24 h, and the gene expression profiles were examin ... | 2014 | 24906993 |
| resolvin e1 reduces hepatic fibrosis in mice with schistosoma japonicum infection. | the aim of this study was to investigate whether resolvin e1 (rve1) protects against hepatic fibrosis in a murine model of liver fibrosis induced by schistosoma japonicum infection. a total of 30 pathogen-free kunming mice were randomly and equally divided into three groups: control (uninfected, untreated), model (infected, untreated) and rve1 intervention (infected, rve1-treated; 100 ng daily). the mice were infected with schistosoma japonicum by inoculating the abdominal skin with 20±2 cercari ... | 2014 | 24926330 |
| a multi-component integrated approach for the elimination of schistosomiasis in the people's republic of china: design and baseline results of a 4-year cluster-randomised intervention trial. | despite major successes in its control over the past 50years, schistosomiasis japonica continues to be a public health problem in the people's republic of china (p.r. china). historically, the major endemic foci occur in the lakes and marshlands along the yangtze river, areas where transmission interruption has proven difficult. the current endemic situation may alter due to the closure of the three gorges dam. considerable environmental and ecological changes are anticipated that may result in ... | 2014 | 24929133 |
| a boswellic acid-containing extract ameliorates schistosomiasis liver granuloma and fibrosis through regulating nf-κb signaling in mice. | boswellic acid (ba)-containing extracts such as bse have anti-inflammatory and immunomodulatory activity. in chronic schistosomiasis, the hepatic granuloma and fibrosis induced by egg deposition in the liver is the most serious pathological manifestations. however, little is known regarding the role of bas in schistosoma japonicum (s. japonicum) egg-induced liver granuloma and fibrosis. in order to investigate the effect of a water-soluble complex preparation of bse, bse-cd, on s. japonicum egg- ... | 2014 | 24941000 |
| effect of ozonide oz418 against schistosoma japonicum harbored in mice. | the in vitro and in vivo efficacies of ozonide carboxylic acid oz418 against schistosoma japonicum were investigated. for in vitro experiments, juvenile (14-day-old) and adult schistosomes were collected from mice infected with 80-100 s. japonicum cercariae for 14 and 35 days post-infection and the worms were maintained in roswell park memorial institute (rpmi) 1640 supplemented by 10% calf serum. against 35-day-old adult s. japonicum, oz418 resulted in weakened worm motor activity, injury to th ... | 2014 | 24948106 |
| first insight into cd59-like molecules of adult fasciola hepatica. | the present study focussed on investigating cd59-like molecules of fasciola hepatica. a cdna encoding a cd59-like protein (termed fhcd59-1) identified previously in the membrane fraction of the f. hepatica tegument was isolated. this homologue was shown to encode a predicted open reading frame (orf) of 122 amino acids (aa) orthologous to human cd59 with a 25 aa signal peptide, a mature protein containing 10 cysteines and a conserved cd59/ly-6 family motif "ccxxxxcn". an analysis of cdnas from tw ... | 2014 | 24955521 |
| comparison of the miracidium hatching test and modified kato-katz method for detecting schistosoma japonicum in low prevalence areas of china. | the diagnosis of schistosoma japonicum (sj) infection in low prevalence areas of the people's republic of china is challenging due to the sensitivity of the detection methods, leading to an underestimation of the disease burden. we compared the sensitivities of the miracidium hatching test (mht) with the modified kato-katz method (kk) and the combination of the two methods (kk-mht) to detect sj infection in low prevalence areas of china. the stool samples of 3,853 residents from 8 villages with ... | 2014 | 24964649 |
| discovery of novel schistosoma japonicum antigens using a targeted protein microarray approach. | novel vaccine candidates against schistosoma japonicum are required, and antigens present in the vulnerable larval developmental stage are attractive targets. post-genomic technologies are now available which can contribute to such antigen discovery. | 2014 | 24964958 |
| evaluation of partially purified soluble egg antigens in colloidal gold immunochromatography assay card for rapid detection of anti-schistosoma japonicum antibodies. | shistosomiasis is one of the important parasitic diseases in developing countries and especially remains a threat to public health in china. many immunodiagnostic kits have shown cross-reactions with other parasitic diseases and need large volumes of serum for the tests. in this study, we evaluated partially purified soluble egg antigen (sea) in a colloidal gold immunochromatography assay (gica) card kit for rapid detection of anti-schistosoma japonicum antibodies using 5 microl of serum. partia ... | 2014 | 24974640 |
| cascaded-automatic segmentation for schistosoma japonicum eggs in images of fecal samples. | to recognize parasite eggs automatically, the automatic segmentation of parasite egg images is very important for the extraction of characteristics and genera classification. | 2014 | 24992730 |
| characteristics of γδ t cells in schistosoma japonicum-infected mouse mesenteric lymph nodes. | gamma delta (γδ) t cells are mainly present in mucosa-associated lymphoid tissues, which play an important role in mucosal immunity. in this study, c57bl/6 mice were infected by schistosoma japonicum and lymphocytes were isolated from the mesenteric lymph node (mln) to identify changes in the phenotype and function of γδ t cells using flow cytometry. our results indicated that the absolute number of γδ t cells from the mlns of infected mice was significantly higher compared with normal mice (p < ... | 2014 | 24994455 |
| resistance to niclosamide in oncomelania hupensis, the intermediate host of schistosoma japonicum: should we be worried? | as the currently only available molluscicide, niclosamide has been widely used for snail control for over 2 decades in china. there is therefore a concern about the emergence of niclosamide-resistant snail populations following repeated, extensive use of the chemical. the purpose of this study was to investigate the likelihood of niclosamide resistance in oncomelania hupensis in china. active adult o. hupensis snails derived from 20 counties of 10 schistosomiasis-endemic provinces of china, of 1 ... | 2015 | 25003984 |
| schistosomiasis japonica: modelling as a tool to explore transmission patterns. | modelling is an important tool for the exploration of schistosoma japonicum transmission patterns. it provides a general theoretical framework for decision-makers and lends itself specifically to assessing the progress of the national control programme by following the outcome of surveys. the challenge of keeping up with the many changes of social, ecological and environmental factors involved in control activities is greatly facilitated by modelling that can also indicate which activities are c ... | 2015 | 25004441 |
| characterisation of a secretory serine protease inhibitor (sjb6) from schistosoma japonicum. | proteins belonging to the serine protease inhibitor (serpin) superfamily play essential physiological roles in many organisms. in pathogens, serpins are thought to have evolved specifically to limit host immune responses by interfering with the host immune-stimulatory signals. serpins are less well characterised in parasitic helminths, although some are thought to be involved in mechanisms associated with host immune modulation. in this study, we cloned and partially characterised a secretory se ... | 2014 | 25023829 |
| schistosoma japonicum infection induces macrophage polarization. | the role of macrophages (mφ) as the first line of host defense is well accepted. these cells play a central role in orchestrating crucial functions during schistosomal infection. thus, understanding the functional diversity of these cells in the process of infection as well as the mechanisms underlying these events is crucial for developing disease control strategies. in this study, we adopted a mφ polarization recognition system. m1 macrophage was characterized by expressing cd16/32, il-12 and ... | 2014 | 25050114 |
| [cloning and function analysis of high mobility group box 1 (hmgb1) protein of schistosoma japonicum (mainland strain)]. | to clone and express a high mobility group box 1(hmgb1) protein of schistosomajaponicum (mainland strain) and analyze its function. | 2014 | 25051827 |
| [cloning and expression of schistosoma japonicum vcp gene and its mrna expression levels in different stages]. | to prokaryotically express the valosin-containing protein (vcp) of schistosoma japonicum, and analyze its vcp mrna expressions in the cercaria, schistosomulum, adult worm (female and male worms) and egg. | 2014 | 25051828 |
| [expression characteristics of microrna in mice with schistosomiasis and praziquantel treatment]. | to investigate the expression characteristics of mir-155 and mir-146a in mice with schistosomiasis and praziquantel (pzq) treatment. | 2014 | 25051829 |
| [dynamic expressions of il-22 and hepatic stellate cells senescence in mice infected with schistosoma japonicum]. | to investigate the dynamic expressions of interleukin-22 (il-22) , interleukin-22 receptor 1 (il-22r1), and hepatic stellate cells (hsc) senescence in mice with schistosoma japonicum infection. | 2014 | 25051830 |
| [jatropha seed oils extracted by different methods and their effect on killing cercaria of schistosoma japonicum]. | to evaluate the effect of jatropha seed oils extracted by different methods on killing cercaria of schistosomajaponicum so as to screen the optimum process and formulations. | 2014 | 25051835 |
| [discovery and biology of schistosoma haematobium]. | schistosomiasis is a neglected tropical disease that severely threatens human health and affects the socioeconomic development. the causative agent that parasitizes in humans mainly involves schistosoma japonicum, s. mansoni, s. haematobiurn, s. intercalatum and s. mekongi. as the firstly identified schistosome, s. haematobium infection is found to strongly correlate with bladder cancer. this paper mainly reviews the discovery, morphology and life cycle of s. haematobium. | 2014 | 25051845 |
| [prokaryotic expression and function analysis of schistosoma japonicum calpain]. | to clone and express recombinant calpain of schistosoma japonicum (sjcalpain), observe the distribution of sjcalpain in s. japonicum cercariae and analyze its role in skin invasion. | 2014 | 25065204 |
| [cloning, expression and transcription specificity analysis of two tyrosinases from schistosoma japonicum]. | to clone and express the recombinant proteins based on the whole open reading frame of two tyrosinases (tyrosinase 1 and tyrosinase 2) from schistosoma japonicum, and study the transcription specificity of the two tyrosinases in different sex and developmental stages of s. japonicum. | 2014 | 25065206 |
| [expression of toll-like receptors 2 and 6 in mice liver during schistosoma japonicum infection]. | to investigate the expression of hepatic toll-like receptor 1 (tlr1), tlr2 and tlr6 on mice with schistosoma japonicum infection. | 2014 | 25065207 |
| [changes of ifn-gamma, il-4 and t cells in schistosoma japonicum-infected mice after praziquantel treatment]. | to investigate the serum levels of ifn-gamma and il-4, and the dynamic changes of ifn-gamma-specific and il-4-specific lymphocytes in mice with schistosoma japonicum infection after treatment by praziquantel. | 2014 | 25065208 |
| saudi guidelines on the diagnosis and treatment of pulmonary hypertension: schistosomiasis and pulmonary arterial hypertension. | schistosomiasis is caused by infection with the parasite schistosoma, which is a flat-worm or fluke. the dominant species are schistosoma mansoni, schistosoma japonicum, and schistosoma haematobium. schistosomiasis is the third most common parasitic disease in the world after malaria and amoebiasis. it is endemic in more than 70 countries affecting about 200 million people worldwide, of whom 80% are in sub-saharan africa. there are pockets of infection in north-eastern brazil, near the yangtze r ... | 2014 | 25076995 |
| cloning, expression and characterisation of a type ii cystatin from schistosoma japonicum, which could regulate macrophage activation. | cystatin play an important role in parasite immune evasion. it is involved in many immune responses processes regulations such as inhibiting antigen presentation, modifying cytokines production and macrophage polarization. in recent years, more and more cystatins were used in treating some inflammatory diseases such as asthma and inflammation bowel diseases; however, cystatins from schistosoma japonicum were rarely studied. in the present study, we have cloned a cystatin from the adult stage of ... | 2014 | 25096535 |
| cd8α¯ dc is the major dc subset which mediates inhibition of allergic responses by schistosoma infection. | our and others' previous studies have shown that schistosoma japonicum (sj) infection can inhibit allergic reactions. we recently reported that dcs played an important role in sj infection-mediated inhibition of allergy, which was associated with enhanced il-10 and t regulatory cell responses. here, we further compared the role of cd8α(+) dc and cd8α(-) dc subsets for the inhibitory effect. we sorted cd8α(+) dc (sjcd8α(+) dc) and cd8α(-) dc (sjcd8α(-) dc) from sj-infected mice and tested their a ... | 2014 | 25099746 |
| involvement of il-13 and tissue transglutaminase in liver granuloma and fibrosis after schistosoma japonicum infection. | schistosomiasis, one of the most devastating parasitic diseases, is caused by schistosoma japonicum (sj) infection resulting in serious liver fibrosis. interleukin- (il-) 13, which is produced by th2 cells, is a critical profibrotic cytokine found in various organs, including the liver. tissue transglutaminase (ttg), a group of multifunctional enzymes, serves a central function in the pathogenesis of chronic liver diseases. however, the relationship between il-13, ttg, and liver fibrosis during ... | 2014 | 25110399 |
| expression of heat shock protein 47, transforming growth factor-beta 1, and connective tissue growth factor in liver tissue of patients with schistosoma japonicum-induced hepatic fibrosis. | summary to detect the expression of pro-fibrotic molecules, such as heat shock protein 47 (hsp47), transforming growth factor-beta 1 (tgf-β1) and connective tissue growth factor (ctgf) in liver specimens, and analyse their correlations with the progression of schistosomal hepatic fibrosis, liver biopsy was performed in 42 chronic schistosomiasis (cs) patients, 16 chronic hepatitis b (chb) patients and five healthy individuals (hi). immunohistochemistry (ihc) analyses displayed that the expressio ... | 2015 | 25111595 |
| molecular characterization of sjbirp, another apoptosis inhibitor, from schistosoma japonicum. | inhibitor of apoptosis proteins (iap) play an important role in the regulation of apoptotic processes and are defined by the presence of baculoviral iap repeat (bir) domains. here, we characterized a cdna fragment (sjbirp) synthesized from the rna of schistosoma japonicum, which was found to contain the bir domain, suggesting that it could encode a potential iap. real-time pcr analyses indicated that sjbirp transcription was detected at several stages of the schistosome's lifecycle, with increas ... | 2014 | 25124726 |
| development of chiral praziquantel analogues as potential drug candidates with activity to juvenile schistosoma japonicum. | a series of chiral praziquantel analogues were synthesized and evaluated against schistosoma japonicum both in vitro and in vivo. all compounds exhibited low to considerable good activity in vivo. remarkably, worm reduction rate of r-3 was 60.0% at a single oral dose of 200mg/kg against juvenile stage of schistosoma japonicum. the target compounds displayed in vivo antischistosomal activity against both schistosoma japonicum and schistosoma mansoni. furthermore, all r-isomers displayed stronger ... | 2014 | 25127102 |
| an ultra-sensitive assay targeting the circulating anodic antigen for the diagnosis of schistosoma japonicum in a low-endemic area, people's republic of china. | the downward trend in prevalence and intensity of schistosoma japonicum infection in the people's republic of china (p.r. china) has reached a level where accurate methods are required for monitoring the national schistosomiasis control programme and to verify whether transmission has been interrupted. we have assessed the prevalence of active s. japonicum infection by use of an up-converting phosphor lateral-flow (ucp-lf) assay for determination of circulating anodic antigens (caa) in urine and ... | 2015 | 25128703 |
| geographical distribution of human schistosoma japonicum infection in the philippines: tools to support disease control and further elimination. | schistosoma japonicum infection is believed to be endemic in 28 of the 80 provinces of the philippines and the most recent data on schistosomiasis prevalence have shown considerable variability between provinces. in order to increase the efficient allocation of parasitic disease control resources in the country, we aimed to describe the small-scale spatial variation in s. japonicum prevalence across the philippines, quantify the role of the physical environment in driving the spatial variation o ... | 2014 | 25128879 |
| immune response of γδt cells in schistosome japonicum-infected c57bl/6 mouse liver. | systematic evaluation of the role of γδt cells during the schistosoma japonicum infection has not been reported, despite the fact that γδt cells contribute to many infectious diseases in innate immunity. therefore, the aim of this study was to observe the properties of γδt cells in the liver of c57bl/6 mice infected by s. japonicum. in this report, using immuno-fluorescent histological analysis, γδt cells were found around hepatic granulomatous. moreover, the flow cytometry results revealed that ... | 2014 | 25130072 |
| functional characterization of sjb10, an intracellular serpin from schistosoma japonicum. | summary serine protease inhibitors (serpin) play essential roles in many organisms. mammalian serpins regulate the blood coagulation, fibrinolysis, inflammation and complement activation pathways. in parasitic helminths, serpins are less well characterized, but may also be involved in evasion of the host immune response. in this study, a schistosoma japonicum serpin (sjb10), containing a 1212 bp open reading frame (orf), was cloned, expressed and functionally characterized. sequence analysis, co ... | 2014 | 25137634 |
| identification, immunolocalization, and characterization analyses of an exopeptidase of papain superfamily, (cathepsin c) from clonorchis sinensis. | cathepsin c is an important exopeptidase of papain superfamily and plays a number of great important roles during the parasitic life cycle. the amino acid sequence of cathepsin c from clonorchis sinensis (c. sinensis) showed 54, 53, and 49% identities to that of schistosoma japonicum, schistosoma mansoni, and homo sapiens, respectively. phylogenetic analysis utilizing the sequences of papain superfamily of c. sinensis demonstrated that cathepsin c and cathepsin bs came from a common ancestry. ca ... | 2014 | 25138068 |