Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| rotaviruses as a cause of nosocomial, infantile diarrhoea in northern brazil: pilot study. | faecal samples were obtained from 190 children, aged 0 to 5 years, admitted to a public hospital in belém, pará, brazil. these patients were placed in a pediatric ward with 40 beds distributed in six rooms. cases were classified into three groups: (a) nosocomial: children who developed gastroenteritis 72 hr or later after admission; (b) community-acquired: patients admitted either with diarrhoea or who had diarrhoea within 72 hr following admission; (c) non-diarrhoeic: those children who had no ... | 1995 | 8731371 |
| use of the prospect microplate enzyme immunoassay for the detection of pathogenic and non-pathogenic entamoeba histolytica in faecal specimens. | a commercial microplate enzyme immunoassay (prospect eia; alexon inc., sunnyvale, ca 94089, usa) was compared with conventional microscopy for the diagnosis of entamoeba histolytica infection. using specimens known to be infected, the sensitivity of the prospect eia was 78% and its specificity was 99%. no cross reaction with other intestinal parasites was observed. the prospect eia and conventional microscopy (using merthiolate-iodine-formaldehyde direct wet mounts and concentration techniques) ... | 1996 | 8758065 |
| rapid in vitro test for determination of anti-amoebic activity. | a rapid microplate assay for the detection of potential compounds active against entamoeba histolytica is described. the assay is based on the metabolic reduction of a tetrazolium salt by e. histolytica trophozoites as an indicator of their viability which may be measured photometrically. the method was validated by determining the dose-response characteristics of standard amoebicides and correlating optical density and cell number; it provides a convenient means of selecting potentially novel a ... | 1996 | 8761587 |
| entamoeba histolytica: fecal antigen capture immunoassay for the diagnosis of enteric amebiasis by a monoclonal antibody. | amebiasis continues to be of epidemiological importance in underdeveloped countries. clinical diagnosis and epidemiological setting in a region are based on the fecal microscopic identification of cysts or trophozoites. this procedure requires well trained personnel, is laborious, of low sensitivity and frequently yields false-positives results. the present study was designed to develop an immunoenzymatic fecal 96 kda antigen capture test (coproelisa-eh) more sensitive and specific than microsco ... | 1996 | 8762638 |
| [the absence of invasive amebiasis in male homosexual aids patients in recife]. | the purpose of this paper was to determine if among male homosexual aids [correction of sida] patients, the entamoeba histolytica was able to product invasive illness. from the 77 investigated patients only one (1.3%) has cysts of e. histolytica in his stools. the electrophoresis of the only isolated strain has shown it was from zimodeme i, non pathogenic. the research of antiamebic antibodies was negative in the serum of the totality of patients. those results showed that even in immunocompromi ... | 1996 | 8768579 |
| production and characterization of murine monoclonal antibodies to blastocystis hominis. | several hybridomas producing antibodies detected by enzyme-linked immunosorbent assay (elisa) were established by fusions of mouse myeloma p3.x63.ag8.u1 with spleen cells from balb/c mice immunized against an isolate of blastocystis hominis. five strongly positive hybrids (6b6, 1d5, 1e7, 4f7 and 4g11) were cloned and all were found to secrete igm monoclonal antibodies. four mabs (6b6, 1e7, 4f7 and 4g11) reacted in immunoblots with a number of b. hominis antigens (mol. wt ranging from 25,000 to 2 ... | 1996 | 8773525 |
| reduction in virulence of naegleria fowleri following growth with cholesterol. | it has been reported that the virulence of axenically cultivated entamoeba histolytica increases following growth with cholesterol. the purpose of this study was to determine whether cholesterol would enhance the virulence of axenically cultivated naegleria fowleri. amoebae were cultivated in axenic medium with (100 micrograms/ml) or without cholesterol for 6 months and tested in mice for changes in virulence. after 6 months of continuous cultivation. n. fowleri grown with cholesterol was less v ... | 1995 | 8774777 |
| comparative evaluation of somatic & excretory-secretory antigens of entamoeba histolytica in serodiagnosis of human amoebiasis by elisa. | the excretory-secretory antigens collected from the axenic culture medium and conventional somatic antigen prepared from the whole trophozoites of e. histolytica were compared for their efficacy in serodiagnosis of amoebiasis. a total of 280 sera collected from different clinically proven cases of amoebiasis and healthy subjects were analysed against both the antigens in elisa. both antigens showed a 100 per cent correlation in amoebic liver abscess cases, patients infected with enteropathogens ... | 1996 | 8783520 |
| partial purification and characterization of ornithine decarboxylase from entamoeba histolytica. | multiplication of e. histolytica was accompanied by a parallel increase in ornithine decarboxylase (odc) specific activity up to 72 h of cultivation in tyi-s-33 medium. thereafter, activity rapidly decayed whereas growth continued for another 24 h before entering into the stationary growth phase. odc was very unstable. partial purification (14-fold) of the enzyme was achieved by a three-step procedure involving high-speed centrifugation, gel filtration and adsorption to hydroxylapatite. the part ... | 1996 | 8786214 |
| molecular evolution of the 14-3-3 protein family. | members of the highly conserved and ubiquitous 14-3-3 protein family modulate a wide variety of cellular processes. to determine the evolutionary relationships among specific 14-3-3 proteins in different plant, animal, and fungal species and to initiate a predictive analysis of isoform-specific differences in light of the latest functional and structural studies of 14-3-3, multiple alignments were constructed from forty-six 14-3-3 sequences retrieved from the genbank and swissprot databases and ... | 1996 | 8798343 |
| sequence-specific detection of pcr-amplified dna by restriction enzyme release of hybrids. | we have developed a method to detect pcr amplicons that contain a restriction enzyme site. the method is called polymerase chain reaction-hybridization and enzymatic release assay (pcr-hera). the pcr product is hybridized to a synthetic oligonucleotide that is immobilized on a microtitre plate. specific cleavage of the resulting hybrid by a restriction endonuclease liberates a fluorescein labeled arm of the immobilized dna. we demonstrate the use of pcr-hera to detect species-specific sequences ... | 1996 | 8799371 |
| entamoeba histolytica: proteinase secretion induced by collagen type i is dependent on cytoskeleton integrity. | proteolytic activities of the protozoan parasite entamoeba histolytica strain hm1:imss and the cytochalasin d-resistant mutant bg-3 were analyzed following stimulation with collagen type i and ca2+, which induces electron-dense associated collagenase secretion. the mutant bg-3 had a protease activity of 73 kda and secretion of total protease activity was not stimulated by collagen type i and ca2+, which produced, in contrast, a 2-fold increase in protease secretion by the parental strain. this c ... | 1996 | 8801549 |
| intestinal parasites among the southeast asian laborers in taiwan during 1993-1994. | a large number of foreign laborers are now working in taiwan, the majority coming from southeast asia where parasitic infections are common. since 1992, monitoring the intestinal parasitic infections of these foreigners has been carried out, particularly to prevent the introduction of some of the parasites into taiwan. this paper reports results obtained during the year 1993-1994. | 1996 | 8803301 |
| relative importance of inositol (1,4,5)trisphosphate and inositol (1,3,4,5)tetrakisphosphate in entamoeba histolytica. | [3h]inositol tetrakisphosphate (ins(1,3,4,5)p4) binding sites which were poorly displaced by unlabelled inositol (1,4,5)-trisphosphate (ins(1,4,5)p3) were detected in membrane fractions of entamoeba histolytica. similarly, unlabelled ins(1,3,4,5)p4 was 30-fold less efficient in displacing [3h]ins(1,4,5)p3 binding. ph sensitivities of binding of the two isomers were markedly different. scatchard analysis of the data revealed single binding sites and similar receptor densities for each of the two ... | 1996 | 8804436 |
| analysis of 69 patients with amebic liver abscess. | all 69 patients with amebic liver abscess that we treated in 1981-1992 were studied retrospectively. men predominated by a 10:1 ratio. of our 227 patients with amebiasis, some 30% yearly had liver involvement. the incidence peaked in 1988, decreasing later but increasing again in 1992. most patients were 30-50 years old, the overall mean age being 45 years (range, 22-79), and decreasing with time. patients with the related factors of travel abroad, positive results of a test for treponema pallid ... | 1996 | 8808427 |
| sequence of a giardia lamblia gene coding for the glycolytic enzyme, pyruvate,phosphate dikinase. | the sequence of the gene coding for pyruvate,phosphate dikinase (ec 2.7.9.1) in giardia lamblia (syn. g. duodenalis) has been established. the deduced amino acid sequence is very similar to all of its known homologs from the protist, entamoeba histolytica, the eubacterium, clostridium symbiosum and plant chloroplasts. phylogenetic reconstruction with neighbor-joining and maximum parsimony methods reveals that the sequences form two clades, one comprising the anaerobic microorganisms, and the oth ... | 1996 | 8813667 |
| a homologue of the cysteine proteinase gene (acp1 or eh-cpp3) of pathogenic entamoeba histolytica is present in non-pathogenic e. dispar strains. | one of the three cysteine proteinase genes, acp1 (or cp 3), has been reported to be missing in non-pathogenic strains of entamoeba histolytica (or entamoeba dispar as recently labeled). unexpectedly, a gene fragment very similar in its sequence (95% homology) to acp1 of pathogenic strains was obtained by use of the polymerase chain reaction from genomic dna and cdna of various cloned non-pathogenic strains as well as in 23 clinical isolates from asymptomatic carriers. the finding of the acp1 hom ... | 1996 | 8813676 |
| upstream regulatory elements controlling expression of the entamoeba histolytica lectin. | entamoeba histolytica genomic organization and putative promoter elements appear to be distinct from both metazoan and better characterized protozoan organisms. the recent development of dna-mediated transfection for e. histolytica enabled characterization of cis-acting promoter elements required for gene expression. a deletion and replacement analysis was conducted on the promoter of an e. histolytica gene encoding the heavy subunit of the n-acetyl-beta-d-galactosamine-specific adhesin (hgl5). ... | 1996 | 8813680 |
| cloning and characterization of the entamoeba histolytica pyruvate: ferredoxin oxidoreductase gene. | 1996 | 8813698 | |
| recombinant antigen-based avidin-biotin microtiter enzyme-linked immunosorbent assay for serodiagnosis of invasive amebiasis. | an immunoscreening approach was used to isolate a strongly positive cdna clone from an entamoeba histolytica hk-9 cdna expression library in the phage vector lambda zap-ii. the 1.85-kb cdna insert was found to be truncated and encoded the cysteine-rich, immunodominant domain of the antigenic 170-kda subunit of the amebal galactose-n-acetylgalactosamine binding lectin. this domain was expressed as a glutathione s-transferase fusion protein in escherichia coli. inclusion bodies of the recombinant ... | 1996 | 8815092 |
| differentiation of korean isolates of entamoeba histolytica from entamoeba dispar. | cysts of entamoeba histolytica are still found from humans in korea, but not all of the cysts are known as pathogenic. the non-pathogenic strain is regarded as a different species, e. dispar. in this study, korean isolates of conventional e. histolytica were subjected for the differentiation by polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) analysis. human stools were screened by routine microscopic examination, and cyst or trophozoite positive stools were in ... | 1996 | 8820737 |
| physical mapping and expression of gene families encoding the n-acetyl d-galactosamine adherence lectin of entamoeba histolytica. | adherence of the enteric protozoan parasite entamoeba histolytica is mediated by an n-acetyl d-galactosamine (galnac)-specific lectin, a heterodimer of heavy (170 kda) and light (35/31 kda) subunits. the gene families encoding the lectin subunits were characterized using clamped homogeneous electric field (chef) gel electrophoresis in the strain hm1:imss. the heavy subunit was shown to be encoded by a family of five hgl genes, which were physically mapped to five distinct hindiii restriction fra ... | 1996 | 8821939 |
| [invasive amebiasis at an institution for the mentally retarded in shizuoka prefecture]. | amebiasis caused by entamoeba histolytica at an institution for mentally retarded in shizuoka prefecture is reported. five of the 50 patients showed e. histolytica cysts in their stools and 4 were positive serologically. the polymerase chain reaction and restriction fragment length polymorphism revealed that the isolates were pathogenic-type e. histolytica. epidemiological analysis revealed that the amebic infection was caused by the abnormal behavior of mentally retarded patients. administratio ... | 1996 | 8822047 |
| a gene highly homologous to acp1 encoding cysteine proteinase 3 in entamoeba histolytica is present and expressed in e. dispar. | we have cloned the complete gene encoding cysteine proteinase 3 in e. histolytica as well as a cdna encoding a highly homologous protein in e. dispar. in addition, we have demonstrated the presence and expression of the cp3 gene in various e. histolytica and e. dispar isolates. since the expression of the gene is rather similar within both entamoeba species, we assume that it does not constitute the proposed virulence factor of e. histolytica. | 1996 | 8825217 |
| detection and characterization of dna polymerase activity in entamoeba histolytica. | dna polymerase activity was detected and characterized in nuclear extracts from trophozoites of entamoeba histolytica. the activity was high at ph 2 to ph 6, but at ph 8 and 10 the activity was very low. the presence of k+ was inhibitory for the activity and a higher concentration of k+ markedly inhibited the activity. magnesium ions (mg2+) were absolutely required for activity and its optimal concentration was 6 to 8 mm. the activity was markedly inhibited by aphidicolin which is an inhibitor o ... | 1996 | 8825452 |
| recent advances in amebiasis. | advancements in our understanding of amebiasis have been rapid over the decade that i have followed this field. what was identified morphologically for years as entamoeba histolytica has been redescribed with modern techniques as a complex of two species, the commensal parasite e. dispar and the pathogenic parasite e. histolytica that is the cause of colitis and liver abscess. antigen detection tests are now available for the rapid detection in stool of the pathogenic species e. histolytica. new ... | 1996 | 8833626 |
| a study of infective aetiology of chronic diarrhoea in children in amritsar. | examination of 150 stool samples from children of less than 3 years of age of chronic diarrhoea showed the presence of various enteropathogens in 58.7% cases. cryptosporidium in pure form, entamoeba histolytica and giardia lamblia were detected in 1.3%, 2% and in 4% cases respectively. in 1.3% children candida albicans was isolated. the predominant bacterial isolate was enteropathogenic escherichia coli (21.4%). salmonella typhimurium, shigella and campylobacter were isolated in 8.6%, 4% and 0.7 ... | 1995 | 8834137 |
| prevalence of intestinal parasites in adult patients with enteropathic aids in north-eastern tanzania. | a six month study was conducted in north-eastern tanzania to determine the prevalence of pathogenic intestinal parasites among adult patients with enteropathic aids. a total of 352 patients were recruited of whom 158 (45%) had chronic diarrhoea. of the 352 patients, 123 (35%) had intestinal parasites. of the 123, 77 (62.6%) patients had chronic diarrhoea. the types of parasites detected were cryptosporidium, isospora belli, strongyloides stercoralis, schistosoma mansoni, trichuris, trichiura, as ... | 1996 | 8840602 |
| incidence of protozoal diarrheal disease in an expatriate community in addis ababa. | the incidence of diarrheal disease due to entamoeba histolytica and giardia lamblia in the united states embassy american population was recorded over a 25 month period. overall case rates of 4.2/100 person-months for amoebiasis and 3.3/100 person-months for giardiasis were distinguished by wide variations based on age group and rainfall. in some circumstances, gender and prior living in africa also correlated with disease incidence. practitioners should look for these entities in american expat ... | 1996 | 8840612 |
| amoebiasis of the female genital tract: a case report. | in 1994, a 56-year-old burmese woman presented with bloody discharge per vaginam had undergone pelvic examination disclosing multiple small ulcerated and friable lesions with contact bleeding at vulva, vagina and cervix. wet smears from vaginal discharge showed typical entamoeba histolytica trophozoites. the patient was treated with metronidazole 750 mg three times daily for 7 days, resulting in complete recovery of the lesions in 10 days. the pathology, risk factors, diagnosis and management of ... | 1996 | 8840708 |
| a single-step immunochromatographic test for the detection of entamoeba histolytica antigen in stool samples. | a rapid, single step, sensitive and specific immunochromatographic test for the detection of e. histolytica antigens in the stool samples is described. monoclonal antibody ac55 specific for e. histolytica was used as capture antibody on nitrocellulose sheet. the same antibody was conjugated to colloidal gold to detect the presence of antigen. in this test, multiple steps of washing, incubation, etc. are not required and there is no need for secondary colour development as gold particles produce ... | 1996 | 8841457 |
| protection against amebic liver abscess formation in the severe combined immunodeficient mouse by human anti-amebic antibodies. | we have used serum from patients with amebic liver abscess to investigate the role of antibody in the prevention of invasive amebiasis using the severe combined immunodeficient (scid) mouse model of entamoeba histolytica infection. the scid mice were passively immunized with serum or purified antibody from patients with amebic liver abscess 24 hr prior to the direct intrahepatic challenge with 10(6) virulent e. histolytica trophozoites. this treatment reduced the mean abscess size in these anima ... | 1996 | 8842124 |
| cytology of gastrointestinal histoplasmosis. a report of two cases with differential diagnosis and diagnostic pitfalls. | gastrointestinal (gi) histoplasmosis is a rare manifestation of histoplasma capsulatum (hc) infection. there are no reports of its cytologic diagnosis in the literature. | 1996 | 8842180 |
| new concepts regarding the pathogenesis of amebiasis. | our understanding of the pathogenesis of amebiasis has progressed significantly since dr. braude's description of the major clinical syndromes of amebic liver abscess. his hypothesis that invasive amebic strains must be resistant to complement-mediated lysis has been confirmed. we have also shown that entamoeba histolytica activates complement by a unique mechanism, i.e., cleavage of c3 by an extracellular cysteine proteinase. cysteine proteinases are important virulence factors encoded by at le ... | 1995 | 8845450 |
| antigen induced eosinophilia protects gerbils (meriones unguiculatus) against experimental amebic abscess of the liver. | while the normal human eosinophil is destroyed in vitro by virulent entamoeba histolytica, notwithstanding the presence of antibodies and complement, activated eosinophils promptly destroy the parasite even though succumbing in the process as well. to study the possible in vivo participation of eosinophils in invasive amebiasis, we compared the induction of experimental amebic abscess of the liver (eaal) in gerbils (meriones unguiculatus) previously made eosinophilic (532 +/- 80 eosinophils/mm3) ... | 1995 | 8845666 |
| [comparative study on serological diagnosis of entamoebiasis histolytica]. | the practicability of four diagnostic methods for entamoebiasis histolytica including micro-gel diffusion precipitin test (mgdp), indirect immunofluorescent antibody test (ifa), indirect hemagglutination test (iha) and enzyme-linked immunosorbent assay (elisa) was evaluated. the serological test methods were compared by using sera obtained from 30 entamoebiasis histolytica patients and 130 normal health individuals. the highest sensitivity was obtained with the method of elisa, followed by ifa a ... | 1996 | 8847819 |
| [recent features of parasites detected from clinical specimens]. | it is generally considered that we have already been through with problems caused by various kinds of parasites which had once raged throughout the country. on the contrary to our common concept, we occasionally encounter some kinds of parasites in a laboratory as well as in clinical fields, which have become unfamiliar to us in these days. parasitic diseases are in the first place, proper and limited to certain local regions, but the present situation has been greatly changed. nowadays, specifi ... | 1996 | 8847822 |
| diagnostic tests for amoebic liver abscess: comparison of enzyme-linked immunosorbent assay (elisa) and counterimmunoelectrophoresis (cie). | the liver abscess is the most frequent extraintestinal complication of intestinal amoebiasis: its diagnosis is suggested by the clinical picture but it must be confirmed by paraclinic tests. themost stringent diagnosis requires identification of e. histolytica. but this is possible only in a few cases. serological tests greatly improve the diagnosis of this severe complication of amoebiasis. we compared the enzyme linfed immunosorbent assay and the counterimmunoelectrophoresis techniques. both t ... | 1996 | 8851212 |
| energy production in entamoeba histolytica: new perspectives in rational drug design. | the amebicidal action of metronidazole is activated when the enzyme pyruvate:ferredoxin oxido-reductase transfers reducing equivalents to the nitro group of the drug. the enzyme is present in entamoeba histolytica and other anaerobic parasites like giardia and trichomonas that lack mitochondria. the selectivity of the drug can be ascribed to the absence of the reductase in the human host. e. histolytica possesses other enzymes involved in glucose catabolism that are interesting for the rational ... | 1996 | 8854380 |
| the ultrastructure of entamoeba histolytica locomotion. | this quantitative ultrastructural survey of e. histolytica locomotion in boyden chambers supports the concept that this parasite is capable of random, chemokinetic and chemotactic motility. an e. histolytica committed to chemotaxis will flatten over the filter, accumulate smaller vacuoles at the front of the cell, and will also project pseudopods and its polarized body towards and alongside the chemoattractant axis, respectively. other cell features such as cell polarization, membrane ruffling, ... | 1996 | 8854387 |
| molecular biology of entamoeba histolytica: a review. | amebiasis is one of the main causes worldwide of morbidity and mortality by parasites. application of recombinant dna technology to the study of entamoeba histolytica is bringing new light into our understanding of this remarkable protozoan parasite and of the disease it causes. new achievements affect the way we approach many essential questions about e. histolytica, from the mechanism of its pathogenicity to the definition of e. histolytica as a separate species from the nonpathogenic e. dispa ... | 1996 | 8854402 |
| physiology and molecular biology of multidrug resistance in entamoeba histolytica. | in this paper, we present the most relevant facts on multidrug resistance (mdr) in the protozoan parasite entamoeba histolytica. mdr in e. histolytica presents characteristics similar to transformed mammalian cells. e. histolytica drug resistant mutants show cross-resistance to several drugs, and as in mammalian cells the resistance is reverted by verapamil. six p-glycoprotein-like genes (ehpgp) have been cloned and characterized. apparently, four of these genes are transcribed in drug-resistant ... | 1996 | 8854404 |
| protozoa. amebiasis. | the intestinal protozoan parasite entamoeba histolytica causes amebic dysentery and amebic liver abscess, and ranks third worldwide among parasitic causes of death. the application of molecular techniques to the study of this organism have led to major advances in understanding the pathophysiology of amebic infection. this article reviews what is currently known about the pathogenesis, clinical manifestations, diagnosis, and treatment of amebiasis. | 1996 | 8863036 |
| differentiation of entamoeba histolytica and e. dispar dna from cysts present in stool specimens by polymerase chain reaction: its field application in the philippines. | it has been established that two distinct species exist within what was originally known as entamoeba histolytica. these are e. dispar and e. histolytica, for the nonpathogenic and pathogenic forms, respectively. differentiation of these two organisms is of great clinical importance since they are morphologically indistinguishable and both forms can infect the human intestinal cavity to different degrees. a simple and rapid dna-extraction method that can be used directly on formalin-fixed stool ... | 1996 | 8875564 |
| immunodominant entamoeba histolytica antigens recognized by serum and intestinal antibodies after local or systemic immunization of mice with glutaraldehyde fixed trophozoites. | we performed an immunoblot analysis of the main e. histolytica proteins recognized by immune sera and intestinal fluids of balb/c mice immunized with glutaraldehyde fixed trophozoites (gft) by intragastric, rectal and intraperitoneal routes, to determine if there were differences in the amebic antigens immunodominantly recognized at mucosal and systemic levels. the antigen patterns recognized by mice immunized via intraperitoneal and rectal routes were complex and similar suggesting that the imm ... | 1996 | 8876658 |
| entamoeba invadens: characterization of cysteine proteinases. | cysteine proteinases have a number of important functions in the life cycle of protozoan parasites. based on our previous studies demonstrating the role of cysteine proteinases in invasion by entamoeba histolytica, we evaluated the cysteine proteinases of e. invadens, a related protozoan which causes invasive disease of reptiles. e. invadens readily encysts in axenic culture and provides a model to investigate the role of cysteine proteinases in encystation. broad bands of approximately 130-230, ... | 1996 | 8888735 |
| putative serine/threonine protein kinase expressed in complement-resistant forms of entamoeba histolytica. | entamoeba histolytica is susceptible to complement attack in its lumen-dwelling state and develops complement resistance during pathogenic tissue invasion. as experimental evidence suggests that this change in phenotype is accompanied by a change in gene expression, we constructed a subtractive cdna library to identify genes involved. poly(a) + rna from complement-sensitive trophozoites was subtracted from single stranded cdna derived from complement-resistant ones. transcripts enriched in the l ... | 1996 | 8892294 |
| cell surface molecules of pathogenic and nonpathogenic entamoeba histolytica and their relation to virulence. | 1996 | 8895796 | |
| phylogenetic position of kinetoplastid protozoa inferred from the protein phylogenies of elongation factors 1alpha and 2. | partial regions of the mrna encoding a major part of translation elongation factor 2 (ef-2) from a kinetoplastid protozoan, trypanosoma cruzi, were amplified by means of polymerase chain reaction and their primary structures were analyzed. the deduced amino acid sequence was aligned with those of other eukaryotic and archaebacterial ef-2s, and the phylogenetic relationships among eukaryotes were inferred by the maximum likelihood (ml) method. ml analyses of ef-2 phylogeny using six different sto ... | 1996 | 8907178 |
| identification of a 35 kda glycoprotein from entamoeba histolytica by sera from patients with amoebic liver abscess and with mouse monoclonal antibody. | in this work, we explored the relevance of a 35 kda glycoprotein (gm) of the outer membrane from e. histolytica in the diagnosis of the amoebic liver abscess (ala) through elisa and immunoblotting. we were interested in defining the relevance of this antigen in the immune response in patients with amoebic liver abscess and in exploring whether the mouse monoclonal antibody against this 35 kda glycoprotein recognises the same epitope. we found that 87% of ala patients had raised antibody levels t ... | 1996 | 8915688 |
| parasitological and bacteriological studies in recurrent diarrhoea in patients with chronic liver diseases. | sixty patients with chronic liver disease complaining of persistent or recurrent diarrhoea where studied. the stools were examined by direct smear, formol ether concentration method and modified ziehl neelsen technique for cryptosporidium in addition to stool cultures for enteric pathogenic bacteria as salmonella, shigella and e. coli. giardia lamblia was the most prevalent pathogen responsible for diarrhoea in 23.3% of cases followed by entamoeba histolytica in 21.6%, schistosoma mansoni in 5% ... | 1996 | 8918043 |
| evaluation of an antigen-capture enzyme immunoassay for detection of entamoeba histolytica in stool samples. | in order to identify the prevalence of entamoeba histolytica in tourists with diarrhoea returning from countries of the developing world, sensitivity and specificity of a commercially available enzyme immunoassay (eia) kit for the detection of entamoeba histolytica coproantigen in stool were evaluated. five hundred seventy-seven specimens from 469 patients were examined by microscopy and eia. sixty-two specimens from 49 patients were considered positive for entamoeba histolytica. compared with m ... | 1996 | 8922579 |
| [amebic liver abscess--personal observations]. | in the period of 1989-1995 seven patients with amebic liver abscess were observed in clinic of infectious diseases of pomeranian medical school in szczecin. the diagnosis has been made on the base of epidemiological data, presence of intrahepatic defect by a scanning procedure of liver (ultrasonography, ct, scintigraphy) and positive serologic test for amebiasis. all patients were male of polish nationality, 29-57 years old, who became ill after travel to africa or india. intestinal amebiasis wa ... | 1996 | 8927739 |
| entamoeba histolytica and entamoeba dispar: differences in numbers and expression of cysteine proteinase genes. | in order to identify molecules that might be responsible for the difference in pathogenicity between the two closely related protozoan parasites entamoeba histolytica and entamoeba dispar, we focussed on cysteine proteinases because this class of enzymes has been considered important for pathogenic tissue destruction. by screening a genomic library derived from an e. histolytica isolate, a total of six distinct genes (ehcp1-ehcp6) encoding typical prepro-forms of cysteine proteinases were identi ... | 1996 | 8930910 |
| codon usage in cryptosporidium parvum differs from that in other eimeriorina. | codon usage of crytosporidium parvum was compared with those of other eimeriorina toxoplasma gondii and eimeria tenella and revealed a biased use of synonymous codons with a preference for nnu (40.0%) and nna (33.4%). there was no close resemblance of the codon usage of c. parvum to t. gondii (correlation coefficient, r = 0.14) or e. tenella (r = 0.14) but it was similar to entamoeba histolytica (r = 0.75) and plasmodium falciparum (r = 0.5). analysis of the codon usage in homologous gene sequen ... | 1996 | 8935947 |
| direct sequencing of the pcr amplified ssu rrna gene of entamoeba dispar and the design of primers for rapid differentiation from entamoeba histolytica. | since 1993, strains of entamoeba histolytica sensu lato have been assigned to 2 species on the basis of clinical, biochemical, immunological and genetic evidence: the pathogenic strains to e. histolytica sensu stricto, the non-pathogenic strains to entamoeba dispar. analysis of the gene encoding for the small subunit ribosomal rna (ssu rdna) supports the existence of 2 species. however, while 3 whole ssu rdna sequences are available in the data bases for e. histolytica, only a partial sequence h ... | 1996 | 8935948 |
| interaction of ls174t human colon cancer cell mucins with entamoeba histolytica: an in vitro model for colonic disease. | colonic mucins secreted by goblet cells protect the colon by preventing the attachment of enteric pathogens to the epithelium. entamoeba histolytica overcomes this protective barrier and causes ulcerations, allowing the parasite to disseminate to the liver and form abscesses. an in vitro model is used to study the interaction between e. histolytica and colonic mucins. | 1996 | 8942726 |
| characterization of ubiquitin genes and -transcripts and demonstration of a ubiquitin-conjugating system in entamoeba histolytica. | we have investigated the genomic organization of entamoeba histolytica ubiquitin and looked for the occurrence of a ubiquitin-conjugating system in this organism. southern blots indicated the presence of > or = 5 ubiquitin-coding regions. one of these, ehubi1, was cloned and sequenced and found to correspond to a monoubiquitin gene; as shown by a polymerase chain reaction, e. histolytica lacked polyubiquitin genes altogether. blots of poly(a)+ rna from exponentially-growing trophozoite cultures ... | 1996 | 8943152 |
| pacsr: a serum replacement for axenic cultivation of entamoeba histolytica. | 1996 | 8944281 | |
| luminal bacteria and proteases together decrease adherence of entamoeba histolytica trophozoites to chinese hamster ovary epithelial cells: a novel host defence against an enteric pathogen. | factors that prevent colonic mucosal invasion by pathogenic entamoeba histolytica are not understood. a key initial step in pathogenesis of injury induced by amoeba is adherence to target cells mediated by a surface glycoprotein lectin on e histolytica. mucin degrading bacteria normally present in the colon lumen produce glycosidases that degrade soluble or cell surface glycoconjugates. | 1996 | 8944559 |
| overexpression of p-glycoprotein gene 1 by transfected entamoeba histolytica confers emetine-resistance. | 1996 | 8946391 | |
| trypanosoma rangeli and trypanosoma cruzi: molecular characterization of genes encoding putative calcium-binding proteins, highly conserved in trypanosomatids. | genes encoding a 29-kda flagellar calcium-binding protein (f29) in trypanosoma cruzi, strongly homologous to ef-hand calcium-binding protein-encoding genes previously reported in this parasite, were isolated by immunoscreening. f29 is encoded by a number of very similar genes, highly conserved among different t. cruzi isolates. the genes are located on a pair of homologous chromosomes, arranged in one or two clusters of tandem repeats. pcr amplification of trypanosoma rangeli genomic dna, using ... | 1996 | 8948328 |
| presence of a mitochondrial-type 70-kda heat shock protein in trichomonas vaginalis suggests a very early mitochondrial endosymbiosis in eukaryotes. | molecular phylogenetic analyses, based mainly on ribosomal rna, show that three amitochondriate protist lineages, diplomonads, microsporidia, and trichomonads, emerge consistently at the base of the eukaryotic tree before groups having mitochondria. this suggests that these groups could have diverged before the mitochondrial endosymbiosis. nevertheless, since all these organisms live in anaerobic environments, the absence of mitochondria might be due to secondary loss, as demonstrated for the la ... | 1996 | 8962101 |
| heterogeneity of entamoeba histolytica rac genes encoding p21rac homologues. | entamoeba histolytica (eh), the parasite that causes amebic dysentery, is the only protozoan that phagocytoses bacteria, epithelial cells and red blood cells. numerous low-molecular weight gtp-binding proteins, called p21rac, are implicated in signal transduction and actin polymerization during phagocytosis by macrophages and dictyostelium discoideum (dd). here, molecular cloning techniques were used to obtain four eh rac genes that encoded putative p21rac, as well as segments of two eh rac pseu ... | 1996 | 8964500 |
| identification and cellular localization of the actin-binding protein abp-120 from entamoeba histolytica. | several actin-binding proteins participate in the morphological changes that occur during amoeboid movement. the gene encoding one of these proteins, the gelation factor abp-120, was identified and characterized from trophozoites of entamoeba histolytica. the sequence contains 2574 nucleotides, with an open reading frame of 858 amino acids, giving a protein of 93 kda belonging to the spectrin family. the n-terminal domain of abp-120 from e. histolytica revealed a consensus site for actin binding ... | 1996 | 8971707 |
| identification of novel genes from entamoeba histolytica by expressed sequence tag analysis. | shotgun sequencing of cdna clones is now an established approach to gain insight into the expressed nucleotide (nt) sequences in a given cell. we analysed 100 randomly picked cdna clones of the protozoan parasite, entamoeba histolytica, by nt sequencing, with a view to obtain novel gene sequences not detected so far by biochemical and genetic analyses. about 56% of the analysed clones showed significant homology with other genes in the database, including a number of genes whose presence may not ... | 1996 | 8973317 |
| identification and analysis of the u6 small nuclear rna gene from entamoeba histolytica. | among the small nuclear rnas (snrnas) involved in the spliceosomal processing of pre-mrna, u6 is the most conserved. as a first evidence for the presence of the splicing machinery in the amitochondrial protozoan entamoeba histolytica (eh), we have cloned the u6 snrna gene. we find that in this organism u6 is a single copy gene that is transcribed as a poly(a)- rna molecule of approximately 105 nucleotides. we have mapped the 5' end of the u6 snrna transcript, and identified typical elements of a ... | 1996 | 8973344 |
| persistent diarrhea caused by isospora belli: therapeutic response to pyrimethamine and sulfadiazine. | a 54-year-old human immunodeficiency virus (hiv)-positive homosexual man developed overwhelming watery diarrhea and marked weight loss over a 3-week period. although entamoeba histolytica and other nonpathogenic enteric protozoa were observed along with isospora belli in this patient's stool specimens, they were promptly eradicated after metronidazole (flagyl) treatment. the presence of i. belli oocysts in various stages of development in the stool and clinical symptoms related to isospora infec ... | 1996 | 8985661 |
| comparable growth of a trichomonas vaginalis strain in pehps and tyi-s-33 media. | pehps medium, developed for axenic cultivation of entamoeba histolytica and e. invadens, was also capable of supporting the growth of a trichomonas vaginalis strain, with an inoculum of 1 to 100 trichomonads/ml. the logarithmic growth phase in pehps or in tyi-s-33 medium lasted 72 h; yield (3.33 +/- 0.56 x 10(6) trichomonads/ml), duplication time (4.27 h), number of duplications (16.85), or increase ratio (33,328) in pehps medium showed no significant differences with those obtained in tyi-s-33 ... | 1996 | 8987196 |
| antioxidant defense mechanisms in parasitic protozoa. | many of the parasitic protozoa, such as entamoeba histolytica, giardia, trypanosoma, leishmania, and plasmodium, are considered to be anaerobes because they can be grown in vitro only under conditions of reduced oxygen tension. however, these parasitic protozoa have been found to be aerotolerant or microaerophilic, and also to consume oxygen to a certain extent. furthermore, these organisms are highly susceptible to exogenous reactive oxygen species, such as hydrogen peroxide. they must, therefo ... | 1996 | 8989515 |
| study of the incidence of intestinal and systemic parasitoses in a group of children with handicaps. | a high incidence of parasitic infections was shown by the study of children with physical and psychic handicaps. in 231 children examined we diagnosed 294 parasitic infections as follows: 42 (18.1%) with giardia intestinalis 6 (2.59%) with entamoeba histolytica 36 (15.5%) with hymenolepis nana 21 (9.9%) with strongyloides stercoralis 16 (6.9%) with ascaris lumbricoides 92 (39.86%) with tricocephalus dispar 38 (16.4%) with enterobius vermicularis 7(3.03%) with cryptosporidium sp. also, by immunod ... | 1995 | 8993115 |
| waterborne protozoan pathogens. | protozoan parasites were the most frequently identified etiologic agents in waterborne disease outbreak from 1991 to 1994. the waterborne parasites giardia lamblia, naegleria fowleri, acanthamoeba spp., entamoeba histolytica, cryptosporidium parvum, cyclospora cayetanesis, isospora belli, and the microsporidia are reviewed. for each parasite, the review includes history, life cycle, incidence, symptoms, and therapy. clinical detection methods are compared, and emerging technologies are discussed ... | 1997 | 8993859 |
| the sequence and organization of the core histone h3 and h4 genes in the early branching amitochondriate protist trichomonas vaginalis. | among the unicellular protists, several of which are parasitic, some of the most divergent eukaryotic species are found. the evolutionary distances between protists are so large that even slowly evolving proteins like histones are strongly divergent. in this study we isolated cdna and genomic histone h3 and h4 clones from trichomonas vaginalis. two histone h3 and three histone h4 genes were detected on three genomic clones with one complete h3 and two complete h4 sequences. h3 and h4 genes were ... | 1996 | 8995053 |
| does non-dysenteric intestinal amoebiasis exist? | chronic abdominal pain and frequent bowel disturbance are common symptoms experienced by more than 15% of apparently healthy people. in areas endemic for entamoeba histolytica infection, these symptoms are often diagnosed as non-dysenteric intestinal amoebiasis even though no causal relation between such symptoms and e histolytica has been established and clinical presentation of non-dysenteric intestinal amoebiasis and irritable bowel syndrome (ibs) is not distinct. this study was done to asses ... | 1997 | 8996420 |
| identification of entamoeba histolytica and e. dispar cysts in stool by polymerase chain reaction. | an attempt to identify cysts of entamoeba histolytica and e. dispar in human stool was conducted by polymerase chain reaction (pcr) using two sets of primers (p11 plus p12 and p13 plus p14) specific for either species of ameba. the cysts in stool specimens obtained from 12 infected individuals were concentrated, freeze-thawed, and treated with triton x-100 before their examination by pcr. the results of pcr on the cysts were generally consistent with data obtained by pcr on ameba trophozoites ha ... | 1997 | 9000244 |
| entamoeba histolytica: gene linkage groups and relevant features of its karyotype. | we identified some gene linkage groups in entamoeba histolytica using a 4-m urea improved transversal alternating field electrophoresis (tafe) method. complex rosette-structured dna molecules were found trapped along the gel lanes, explaining the fuzziness of the patterns. using several episomal probes, including 16 s, 5.8 s, and 25 s ribosomal (r)dna genes, an autonomous replication sequence (ars), and ehvr1, we identified a complete ribosomal episome linkage group (celg) at the 1.2-mb position ... | 1996 | 9003315 |
| [synthesis and antiparasitic activity of new n-alkyl derivatives of 1-(nitrophenyl)-3-(methyl-3-indolyl)-prop-2-ene-1-ones (nitroindolylchalcones)]. | 1996 | 9008902 | |
| [impact of new methodologies in the study of parasites]. | 1996 | 9011513 | |
| differentiation of entamoeba histolytica and entamoeba dispar in cyst-passers by immunoblot. | differentiation of invasive strains of entamoeba histolytica according to their pathogenicity has been a topic of long debate, but now the pathogenic species only is regarded as e. histolytica while the non pathogenic species is e. dispar. the present study applied immunoblot to differentiate infections of the two species among microscopically-detected cyst-passers in korea. the crude extract of e. histolytica separated in 5.20% gradient gels, revealed many fractions of 94, 81, 71, 50, 44, 38.5, ... | 1996 | 9017910 |
| does anti-parasitic treatment normalize platelets morphology in patients infested with entamoeba histolytica? | platelets are part of body defence system, especially the anti-parasite immunologic response. platelets manifest their functions only after their activation. thrombine activates platelets inducing change of their shape and causing secretion of certain substances. this study was designed to estimate platelet morphology as an indicator of their activation and effectiveness of anti-parasite therapy. the study was conducted in group of 30 patients infested with e. histolytica before treatment (a) an ... | 1996 | 9020537 |
| rapid postmortem invasion of cecal mucosa of macaques by nonpathogenic entamoeba chattoni. | although entamoeba histolytica is the third leading parasitic cause of death in the world, most infections in humans are asymptomatic and restricted to the intestinal lumen. entamoeba histolytica infections have also been reported in most species of captive nonhuman primates, with new world monkeys being particularly susceptible to fatal invasive amebiasis. in contrast, old world monkeys appear to be resistant to the disease, although tissue invasion in asymptomatic monkeys has been reported. ou ... | 1996 | 9025684 |
| seroepidemiology of entamoeba histolytica in a slum in northeastern brazil. | infection with the human pathogenic parasite entamoeba histolytica has not been well-characterized in northeastern brazil. in this study, the prevalence of e. histolytica infection in a slum in northeastern brazil was assayed using an enzyme-linked immunosorbent assay (elisa) for antibodies against the galactose/n-acetyl-d-galactosamine (gal/galnac)-inhibitable adherence lectin of e. histolytica. sera from a total of 335 individuals were examined for anti-gal/galnac lectin antibodies. the overal ... | 1996 | 9025700 |
| stable episomal transfection and gene expression in entamoeba dispar. | 1996 | 9027760 | |
| pyruvate: ferredoxin oxidoreductase from entamoeba histolytica recognized by a monoclonal antibody. | a mouse monoclonal antibody, eh208c2-2 mab, raised against whole cell antigens of entamoeba histolytica trophozoites of the pathogenic strain hm-1: imss and polyclonal antisera (pab) against membrane antigens of e. histolytica trophozoites of strain hth-56: mutm were screened against a cdna library of the pathogenic strain, sfl3. the monoconal antibody detected many phage plaques expressing an e. histolytica protein. the dna sequence encoding the protein was approximately 55% identical, over 1,1 ... | 1996 | 9031403 |
| a 964 bp repetitive dna in entamoeba histolytica is associated with linear "chromosomal" dnas of variable sizes. | 1997 | 9032991 | |
| identification of putative linear and circular dna molecules in the entamoeba histolytica molecular karyotype. | 1997 | 9032992 | |
| organization of entamoeba histolytica ribosomal and structural genes in pfge. | 1997 | 9032993 | |
| in situ location of the ehredox and 16s rdna genes in entamoeba histolytica trophozoites. | 1997 | 9032994 | |
| molecular cloning of histone h1 in entamoeba histolytica and histone h2b in entamoeba histolytica and entamoeba dispar. | 1997 | 9032995 | |
| detection of a human p53-like protein in e. histolytica. | 1997 | 9032996 | |
| the sequence of a highly homologous gene to hgl2* of entamoeba histolytica that is present and expressed in e.dispar. | 1997 | 9032997 | |
| localization of a retinoblastoma-like protein in entamoeba histolytica. | 1997 | 9032998 | |
| a model for unscheduled dna replication in entamoeba histolytica trophozoites. | 1997 | 9032999 | |
| phenotype variability and genetic polymorphism in entamoeba histolytica clonal populations. | 1997 | 9033000 | |
| possible role of transcribed intergenic sequences in entamoeba histolytica. | 1997 | 9033001 | |
| inducible gene expression in entamoeba histolytica mediated by a tetracycline responsive prokaryotic repressor. | 1997 | 9033002 | |
| establishment of an inducible expression system in entamoeba histolytica. | 1997 | 9033003 | |
| upstream regions of rp l21 genes play a role in regulation of expression at the post-transcriptional level in e. histolytica and e. dispar. | 1997 | 9033004 | |
| control of gene expression in entamoeba histolytica by a cis-acting upstream regulatory element. | 1997 | 9033005 | |
| three conserved cis-acting sequences in the core promoter control gene expression in the protozoan parasite entamoeba histolytica. | 1997 | 9033006 |