Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| mutations in the gerp locus of bacillus subtilis and bacillus cereus affect access of germinants to their targets in spores. | the gerp1 transposon insertion mutation of bacillus cereus is responsible for a defect in the germination response of spores to both l-alanine and inosine. the mutant is blocked at an early stage, before loss of heat resistance or release of dipicolinate, and the efficiency of colony formation on nutrient agar from spores is reduced fivefold. the protein profiles of alkaline-extracted spore coats and the spore cortex composition are unchanged in the mutant. permeabilization of gerp mutant spores ... | 2000 | 10715007 |
| bacillus cereus fatal bacteremia and apparent association with nosocomial transmission in an intensive care unit. | bacillus cereus has sometimes been implicated in food poisoning and in opportunistic infections of seriously ill patients. this report describes an unusual case of persistent bacteremia and multiple organ failure associated with b. cereus in a patient admitted to our institution for lung cancer. the patient was undergoing treatment with an antimicrobial agent (imipenem) that was shown to be effective against the micro-organism in vitro. no portal of entry for the strain was detected. after treat ... | 2000 | 10716089 |
| characterization of vim-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a pseudomonas aeruginosa clinical isolate in france. | pseudomonas aeruginosa col-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in marseilles, france, in 1996. this strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. the carbapenem-hydrolyzing beta-lactamase gene of p. aeruginosa col-1 was cloned, sequenced, and expressed in escherichia coli dh10b. the deduced 266-amino-a ... | 2000 | 10722487 |
| the choline binding site of phospholipase c (bacillus cereus): insights into substrate specificity. | the phosphatidylcholine-preferring phospholipase c from bacillus cereus (plc(bc)) is a 28.5 kda enzyme with three zinc ions in its active site. the roles that a number of amino acid residues play as zinc ligands and in binding and catalysis have been elucidated. recent mechanistic studies indicate that the rate of the reaction is limited by a proton-transfer step during chemical hydrolysis and not substrate binding or product release. an x-ray structure of plc(bc) complexed with a phosphonate in ... | 2000 | 10727235 |
| gene cloning, nucleotide sequencing, and purification and characterization of the d-stereospecific amino-acid amidase from ochrobactrum anthropi sv3. | the gene encoding the d-stereospecific amino-acid amidase from ochrobactrum anthropi sv3 was cloned and sequenced. analysis of 7.3 kb of genomic dna revealed the presence of six orfs, one of which (daaa) encodes the d-amino-acid amidase. this enzyme, daaa, is composed of 363 amino-acid residues (molecular mass 40 082 da), and the deduced amino-acid sequence exhibits homology to alkaline d-peptidase from bacillus cereus df4-b (32% identity), dd-peptidase from streptomyces r61 (29% identity), and ... | 2000 | 10727942 |
| growth of bacillus cereus in pasteurized milk products. | 1999 | 10728618 | |
| ethnomedical aspects of the commonly used toothbrush sticks in ethiopia. | to determine the botanical identity, cytotoxicity, and antibacterial property of the commonly used toothbrush sticks in ethiopia. | 1999 | 10734528 |
| enterocin 012, a bacteriocin produced by enterococcus gallinarum isolated from the intestinal tract of ostrich. | enterococcus gallinarum strain 012, isolated from the duodenum of ostrich, produced enterocin 012 which is active against ent. faecalis, lactobacillus acidophilus, lact. sake, listeria innocua, propionibacterium acidipropionici, propionibacterium sp., clostridium perfringens, pseudomonas aeruginosa and salmonella typhimurium. one of the four pathogenic strains of escherichia coli isolated from the intestinal tract of ostrich was inhibited by enterocin 012. no antimicrobial activity was recorded ... | 2000 | 10736005 |
| an algorithm for automated bacterial identification using matrix-assisted laser desorption/ionization mass spectrometry. | an algorithm for bacterial identification using matrix-assisted laser desorption/ionization (maldi) mass spectrometry is being developed. this mass spectral fingerprint comparison algorithm is fully automated and statistically based, providing objective analysis of samples to be identified. based on extraction of reference fingerprint ions from test spectra, this approach should lend itself well to real-world applications where samples are likely to be impure. this algorithm is illustrated using ... | 2000 | 10740862 |
| development of a fluorogenic probe-based pcr assay for detection of bacillus cereus in nonfat dry milk. | a fluorogenic probe-based pcr assay was developed and evaluated for its utility in detecting bacillus cereus in nonfat dry milk. regions of the hemolysin and cereolysin ab genes from an initial group of two b. cereus isolates and two bacillus thuringiensis isolates were cloned and sequenced. three single-base differences in two b. cereus strains were identified in the cereolysin ab gene at nucleotides 866, 875, and 1287, while there were no species-consistent differences found in the hemolysin g ... | 2000 | 10742226 |
| effect of lysozyme on the lectin-mediated phagocytosis of bacillus cereus by haemocytes of the cockroach, blaberus discoidalis. | lysozyme-like activity has been demonstrated in both cell-free haemolymph and, more abundantly, in haemocyte-lysate supernatants of blaberus discoidalis. this activity was non-inducible, but heat-stable, with a maximum activity at ph 6.2. when b. cereus was pre-incubated in a concentration of chicken egg-white lysozyme equivalent to the concentration of lysozyme-like activity in cell-free haemolymph, the phagocytosis of b. cereus opsonized with glcnac-specific lectins, i.e. bdl2, wga and hpa, wa ... | 2000 | 10742514 |
| epidemiology and pathogenesis of bacillus cereus infections. | bacillus cereus is a causative agent in both gastrointestinal and in nongastrointestinal infections. enterotoxins, emetic toxin (cereulide), hemolysins, and phoshpolipase c as well as many enzymes such as beta-lactamases, proteases and collagenases are known as potential virulence factors of b. cereus. a special surface structure of b. cereus cells, the s-layer, has a significant role in the adhesion to host cells, in phagocytosis and in increased radiation resistance. interest in b. cereus has ... | 2000 | 10742691 |
| genetic structure of population of bacillus cereus and b. thuringiensis isolates associated with periodontitis and other human infections. | the genetic diversity and relationships among 35 bacillus cereus and bacillus thuringiensis isolates recovered from marginal and apical periodontitis in humans and from various other human infections were investigated using multilocus enzyme electrophoresis. the strains were isolated in norway, except for three strains isolated from periodontitis patients in brazil. the genetic diversity of these strains was compared to that of 30 isolates from dairies in norway and finland. allelic variation in ... | 2000 | 10747152 |
| improvement of the detection of listeria monocytogenes by the application of aloa, a diagnostic, chromogenic isolation medium. | a new selective agar medium, aloa, for the selective and differential isolation of listeria monocytogenes has been evaluated. all stressed cultures of l. monocytogenes serovars tested grew on the medium as bluish colonies surrounded by a distinctive opaque halo and gave a productivity ratio of at least 0.95. non-pathogenic listeria sp. produced bluish colonies without a halo as was also the case for some enterococci and bacilli. special attention must be paid to some bacillus cereus strains and ... | 2000 | 10747223 |
| purification and characterization of neutral sphingomyelinase from helicobacter pylori. | phospholipase activities of human gastric bacterium, helicobacter pylori, are regarded as the pathogenic factors owing to their actions on epithelial cell membranes. in this study, we purified and characterized neutral sphingomyelinase (n-smase) from the superficial components of h. pylori strains for the first time. n-smase was purified 2083-fold with an overall recovery of 37%. the purification steps included acid glycine extraction, ammonium sulfate precipitation, cm-sepharose, mono-q, and se ... | 2000 | 10769141 |
| [taxonomy of denitrifying bacteria in soddy podzolic soil]. | the taxonomic composition of denitrifying bacteria in soddy podzolic soil was studied by the succession analysis method. this method revealed a significant variation in the taxonomic composition of denitrifying microorganisms in the course of succession. in contrast to succession analysis, the single microbiological analysis of soil samples reflected only the late stage of succession and thus led to an underestimation of the major members of succession. myxobacteria were found to be the most act ... | 2000 | 10776633 |
| characterization of chitinases excreted by bacillus cereus ch. | bacillus cereus ch was shown to excrete chitinases into the culture supernatant when cultivated in a medium containing 0.2% colloidal chitin, whereas the removal of colloidal chitin resulted in a low activity. after concentration of the culture supernatant by precipitation with ammonium sulfate, the induced chitinases were purified by sequential chromatography. four different chitinases, a, b1, b2, and b3 with molecular masses of 35, 47, 58, and 64 kda, respectively, were separated. all chitinas ... | 2000 | 10779874 |
| macrophage-released proteoglycans enhance ldl aggregation: studies in aorta from apolipoprotein e-deficient mice. | aggregated low-density lipoprotein (ldl) was shown to be present in the atherosclerotic lesion, but the mechanism responsible for its formation in vivo is not known yet. to find out whether ldl aggregation occurs in the arterial wall during atherogenesis, ldls were extracted from the aortas of apolipoprotein e-deficient (e(0)) mice during their aging (and the development of atherosclerosis), and were analyzed for their aggregation states, in comparison to ldls isolated from aortas of control mic ... | 2000 | 10781639 |
| high incorporation of l-amino acids to cereulide, an emetic toxin from bacillus cereus. | cereulide is a principal toxin causing emetic syndrome produced by bacillus cereus. this paper deals with biosynthetic studies on this unusual cyclic depsipeptide toxin from 13c labeled l-amino acid precursors (val, leu, ala) upon cultivation in synthetic media. the analyses were made at atomic level of the constituent amino- or oxy-acids through nmr and esi-ms/ms spectroscopic methods on cereulide and its hydrolysate dipeptides. the incorporation of the 13c atom was 95% in each o-val, o-leu and ... | 2000 | 10782675 |
| products from the incomplete metabolism of pyrene by polycyclic aromatic hydrocarbon-degrading bacteria. | pyrene is a regulated pollutant at sites contaminated with polycyclic aromatic hydrocarbons (pah). it is mineralized by some bacteria but is also transformed to nonmineral products by a variety of other pah-degrading bacteria. we examined the formation of such products by four bacterial strains and identified and further characterized the most apparently significant of these metabolites. pseudomonas stutzeri strain p16 and bacillus cereus strain p21 transformed pyrene primarily to cis-4,5-dihydr ... | 2000 | 10788360 |
| production and characterization of monoclonal antibodies against vegetative cells of bacillus cereus. | two monoclonal antibodies (mabs) against bacillus cereus were produced. the mabs (8d3 and 9b7) were selected by enzyme-linked immunosorbent assay for their reactivity with b. cereus vegetative cells. they reacted with b. cereus vegetative cells while failing to recognize b. cereus spores. immunoblotting revealed that mab 8d3 recognized a 22-kda antigen, while mab 9b7 recognized two antigens with molecular masses of approximately 58 and 62 kda. the use of mabs 8d3 and 9b7 in combination to develo ... | 2000 | 10788418 |
| effect of temperature on growth characteristics of bacillus cereus tz415. | the effect of temperature on the maximal specific growth rate was studied in bacillus cereus between 5 and 40 degrees c cultivated in courgette broth and rich medium (j broth). b. cereus grown from 5 to 38 degrees c in rich medium. no growth was observed in courgette broth below 10 degrees c. the arrhenius plot was fitted from experimental data of b. cereus grown in rich medium and at regulated ph, oxygen and temperature. two domains which are separated by a critical temperature around 13 degree ... | 2000 | 10791720 |
| taking injuries of surviving bacteria into account for optimising heat treatments. | the main assets of early conventional models applied in the field of canned food industries are their simplicity and their robustness. moreover, a certain standardisation of these models allows the intrinsic quantification of a food process like sterilisation, regardless of the nature of concerned microbial populations. however, a first drawback of conventional models is their monofactorial nature: only temperature is considered for the evaluation of microbial heat resistance. a second limit of ... | 2000 | 10791740 |
| relationship between the apparent heat resistance of bacillus cereus spores and the ph and nacl concentration of the recovery medium. | conventional heat resistance data, d values, were previously established by other workers at optimal condition for spores outgrowth. however, in canned food conditions of outgrowth are generally suboptimal in term of ph, salt concentration, water activity. the combined effects of ph and nacl level of the recovery medium for the d value and z(ph) value were studied. spores of bacillus cereus were heated at 95 degrees c in phosphate-citrate buffer media at ph 7. cells were recovered at 25 degrees ... | 2000 | 10791747 |
| prevalence, characterization and growth of bacillus cereus in commercial cooked chilled foods containing vegetables. | in cooked-chilled and pasteurized vegetable products, initial numbers of bacillus cereus were below 10 cfu g-1. before the appearance of spoilage, numbers reached 6-8 log cfu g-1 at 20 degrees c and 4-6 log cfu g-1 at 10 degrees c. bacillus cereus was not detected in samples stored at 4 degrees c. ten percent of strains isolated from the products were able to grow at 5 degrees c and 63% at 10 degrees c. bacillus cereus strains unable to degrade starch, a feature linked to the production of emeti ... | 2000 | 10792519 |
| molecular characterization of the thermomonospora curvata agla gene encoding a thermotolerant alpha-1,4-glucosidase. | the cloning, sequencing and structural characterization of a gene encoding a thermostable alpha-1,4-glucosidase from thermomonospora curvata is described. dna sequence analysis revealed four open reading frames designated agla, aglr, agle and aglf. the agla gene encodes a thermostable alpha-1,4-glucosidase from t. curvata and is situated between two genes, aglr and agle. genes agla, agle and aglf are transcribed in the same direction, while aglr is transcribed in the opposite direction. by compa ... | 2000 | 10792537 |
| epidemiological typing of bacillus cereus by amplified fragment length polymorphism. | a simple amplified fragment length polymorphism method was developed for the epidemiological typing of bacillus cereus. the method was applied to 21 cultures from seven food poisoning and eight non-food poisoning incidents. results were compared with those obtained by conventional serotyping using flagellar antigens and assessed in relation to epidemiological data. amplified fragment length polymorphism was found to be highly reproducible and 16 different profiles (each unique to the 15 incident ... | 2000 | 10792663 |
| hybrid isolation by recovery of rna-dna hybrids from agar using s1 nuclease. | a method for recovering rna-dna hybrids from agar employing a single strand specific nuclease is described. the procedure is suitable for large scale isolations, and immobilization of the dna in agar prior to hybridization allows a high yield of hybrid without interference by dna reannealing. | 1974 | 10793757 |
| production of recombinant l-leucine dehydrogenase from bacillus cereus in pilot scale using the runaway replication system e. coli[piet98]. | a method for the production of recombinant l-leucine dehydrogenase from bacillus cereus in pilot scale is described employing the temperature induced runaway replication vector piet98 and the escherichia coli host strain bl21. fed-batch cultivation using a semi-synthetic high-cell densitiy medium was adjusted in 5-l scale to yield a constant growth rate of 0,17 h(-1) and a final cell concentration of 27 g dry weight/l by exponentially increasing the nutrient supply. runaway replication and thus, ... | 2000 | 10797242 |
| biological screening of brazilian medicinal plants. | in this study, we screened sixty medicinal plant species from the brazilian savanna ("cerrado") that could contain useful compounds for the control of tropical diseases. the plant selection was based on existing ethnobotanic information and interviews with local healers. plant extracts were screened for: (a) molluscicidal activity against biomphalaria glabrata, (b) toxicity to brine shrimp (artemia salina l.), (c) antifungal activity in the bioautographic assay with cladosporium sphaerospermum a ... | 2000 | 10800195 |
| [prevalence of bacterial contamination of standard platelet units: prospective study]. | platelet concentrates contaminated with bacteria are a main source of transfusion-associated sepsis. several studies have reported a very wide incidence (0-10%) of contamination. the aim of this study is to assess the prevalence of bacterial contamination of standard platelet units at the regional blood transfusion center in casablanca. | 2000 | 10812660 |
| synthesis and antibacterial activity of some new non-proteinogenic amino acids containing thiazole residues. | some new thioamides and thiazoles have been synthesized using canavanine, s-cysteine, homo-s-cysteinesulfonamides and their n-omega aminoethylated derivatives as adducts in order to investigate the structure-antimicrobial activity relationships. the compounds showed substantial antibacterial activity in vitro against various gram-positive (staphylococcus aureus, bacillus cereus etc.) and gram-negative (escherichia coli, proteus vulgaris etc.) bacteria. these findings indicate that the presence o ... | 2000 | 10817409 |
| triterpene saponins from the fruits of hedera helix. | six triterpene saponins, including two new compounds, were isolated from the fruits of hedera helix l. (araliaceae). the structures of the new compounds, named helixosides a and b, were established as 3-o-beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranosyl hederagenin 28-o-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl ester, and 3-o-beta-d-glucopyranosyl-(1-->2)-beta-d-glucopyranosyl oleanolic acid 28-o-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl ester, respectively, on the basis o ... | 2000 | 10820801 |
| structure and absolute configuration of new acetylenic compounds isolated from cultures of clitocybe catinus. | investigations of the extracts of a culture of clitocybe catinus gave rise to the isolation of new acetylenic diols 1-3. their structure was determined on the basis of 1h- and 13c-nmr evidence and the absolute configuration elucidated by means of the modified mosher's method. | 2000 | 10820836 |
| effects of pertussis toxin on extracellular signal-regulated kinase activation in hepatocytes by hormones and receptor-independent agents: evidence suggesting a stimulatory role of g(i) proteins at a level distal to receptor coupling. | it was previously found that pertussis toxin (ptx) pretreatment inhibits the activation of extracellular signal-regulated kinases erk1 (p44(mapk)) and erk2 (p42(mapk)) in hepatocytes in response to either agonists that bind to heptahelical receptors or epidermal growth factor (egf), suggesting a role of g(i) proteins in stimulatory mechanisms for erk1/2. the present work shows that erk1/2 is activated in a ptx-sensitive way not only by vasopressin, angiotensin ii, prostaglandin (pg) f(2alpha), a ... | 2000 | 10825231 |
| antimicrobial activity of carvacrol toward bacillus cereus on rice. | the antimicrobial activity of carvacrol, a compound present in the essential oil fraction of oreganum and thyme, toward the foodborne pathogen bacillus cereus on rice was studied. carvacrol showed a dose-related inhibition of growth of the pathogen. concentrations of 0.15 mg/g and higher inhibited the growth and the extent of inhibition depended on the initial inoculum size. to decrease the input of carvacrol on the taste and flavor of the product, a combined treatment with the structure analog ... | 2000 | 10826719 |
| catalytic mechanism of an abzyme displaying a beta-lactamase-like activity. | a catalytic igg (ab2) displaying a beta-lactamase-like activity was previously obtained by using the antiidiotypic pathway: the particularity of this antibody is that it is a true antiidiotype of the beta-lactamase active site. we have previously demonstrated that this igg has retained some of the structural information displayed by the beta-lactamase active site, evident from data that polyclonal anti-ab2 antibodies (ab3) recognize beta-lactamase. in this article, we investigated the catalytic ... | 2000 | 10826957 |
| recovery of bacillus thuringiensis from marine sediments of japan. | marine sediments from a japanese bay were examined for the occurrence of bacillus thuringiensis. of 1313 colonies belonging to the bacillus cereus/b. thuringiensis group, 22 (1.7%) were allocated to b. thuringiensis. marine isolates of b. thuringiensis consisted of heterogeneous multiple h serogroups; 10 isolates were assigned to the eight serovars (kurstaki, sumiyoshiensis, sotto, aizawai, darmstadiensis, thompsoni, neoleonensis, and higo); two motile isolates failed to react with the reference ... | 2000 | 10827286 |
| bacillus anthracis, bacillus cereus, and bacillus thuringiensis--one species on the basis of genetic evidence. | bacillus anthracis, bacillus cereus, and bacillus thuringiensis are members of the bacillus cereus group of bacteria, demonstrating widely different phenotypes and pathological effects. b. anthracis causes the acute fatal disease anthrax and is a potential biological weapon due to its high toxicity. b. thuringiensis produces intracellular protein crystals toxic to a wide number of insect larvae and is the most commonly used biological pesticide worldwide. b. cereus is a probably ubiquitous soil ... | 2000 | 10831447 |
| tripartite haemolysin bl: isolation and characterization of two distinct homologous sets of components from a single bacillus cereus isolate. | haemolysin bl (hbl), a three-component enterotoxic/necrotizing/vascular permeability toxin, is a likely virulence factor of bacillus cereus diarrhoeal food poisoning and necrotic infections. this paper describes the isolation of two distinct homologous sets of all three hbl components from a single b. cereus isolate, mgbc 145. the proteins of one set (designated hbl, consisting of b, l(1) and l(2)), were about 87-100% identical in n-terminal amino acid sequences to their respective prototype com ... | 2000 | 10846215 |
| potentiation of the lethal effect of peroxygen on bacillus cereus spores by alkali and enzyme wash. | bacillus cereus present in pipes and heat-exchangers represents a potential quality problem for dairy industry. the peroxygen-containing disinfectants investigated had only negligible sporicidal effect when applied at the recommended in-use temperature and concentration. however, cleaning agents used before disinfection potentiated their lethal activity. pre-exposure of b. cereus spores to 1% sodium hydroxide at temperatures over 40 degrees c increased the sporicidal effect of the peroxygen-cont ... | 2000 | 10857927 |
| effects of intravitreal corticosteroids in the treatment of bacillus cereus endophthalmitis. | to investigate whether intravitreal corticosteroid therapy reduces the extent of inflammatory intraocular tissue damage caused by bacillus cereus endophthalmitis. | 2000 | 10865318 |
| phenotypic and genotypic comparisons of 23 strains from the bacillus cereus complex for a selection of known and putative b. thuringiensis virulence factors. | sixteen bacillus thuringiensis, four bacillus cereus and three bacillus anthracis isolates were screened for a selection of known and putative b. thuringiensis virulence factors. pcr primers were designed to detect genes for phosphatidylcholine specific phospholipase c, phosphatidylinositol specific phospholipase c, immune inhibitor a, vegetative insecticidal protein 3a, a protein proposed to be involved in capsule synthesis, a newly identified ser/thr kinase homologue and enterotoxin ents. moti ... | 2000 | 10867226 |
| vrrb, a hypervariable open reading frame in bacillus anthracis. | bacillus anthracis appears to be the most molecularly homogeneous bacterial species known. extensive surveys of worldwide isolates have revealed vanishingly small amounts of genomic variation. the biological importance of the resting-stage spore may lead to very low evolutionary rates and, perhaps, to the lack of potentially adaptive genetic variation. in contrast to the overall homogeneity, some gene coding regions contain hypervariability that is translated into protein variation. during marke ... | 2000 | 10869077 |
| lupene-type triterpenes from periploca aphylla. | two new lupane derivatives, 3beta,6alpha-dihydroxylup-20(29)-ene (1) and 6alpha-hydroxylup-20(29)-en-3beta-octadecanoate (2), have been isolated from the stems of periploca aphylla, in addition to beta-sitosterol and lupeol. the structures of 1 and 2 were determined by spectral and chemical methods. compound 1 showed strong inhibition of alpha-glucosidase type vi and a moderate antibacterial activity. | 2000 | 10869229 |
| human placenta sphingomyelinase, an exogenous acidic ph-optimum sphingomyelinase, induces oxidative stress, glutathione depletion, and apoptosis in rat hepatocytes. | ceramide has been identified as a putative lipid messenger that mediates diverse cellular processes including cell death. since glutathione (gsh) depletion is known to sensitize cells to many cytotoxic agents and as a result of the reported regulation of neutral sphyngomyelinase (nsmase) by gsh, the present study compared the role of individual smases in the induction of oxidative stress, regulation of cellular gsh, and apoptosis of rat hepatocytes. exposure of cultured rat hepatocytes to exogen ... | 2000 | 10869289 |
| control of bacterial spores. | bacterial spores are much more resistant than their vegetative counterparts. the most dangerous spore-former is clostridium botulinum which produces a potent neurotoxin that can prove fatal. the most common food poisoning from a spore-former is caused by c. perfringens. other food poisoning spore-formers include bacillus cereus, b. subtilis and b. licheniformis. there are a number of non-pathogenic spore-formers including butyric and thermophilic anaerobes that cause significant economic losses ... | 2000 | 10885113 |
| kombucha fermentation and its antimicrobial activity. | kombucha was prepared in a tea broth (0.5% w/v) supplemented with sucrose (10% w/v) by using a commercially available starter culture. the ph decreased steadily from 5 to 2.5 during the fermentation while the weight of the "tea fungus" and the od of the tea broth increased through 4 days of the fermentation and remained fairly constant thereafter. the counts of acetic acid-producing bacteria and yeasts in the broth increased up to 4 days of fermentation and decreased afterward. the antimicrobial ... | 2000 | 10888589 |
| food safety implication: certain antibiotics may rapidly contaminate egg albumen during the process of its formation. | 1. egg white formation occurs in 3 phases: synthesis and storage of albumen proteins prior to ovulation, secretion of proteins during passage of the ovum down the reproductive tract (preplumping) and addition of water (plumping phase). 2. this study was to determine if oxytetracycline would transfer into egg albumen during the latter 2 phases of albumen formation. 3. in 2 experiments 48 hens were injected with either 400 mg/kg oxytetracycline or physiological saline. hens were dosed at 0.5 h (pr ... | 2000 | 10890213 |
| photochemical inactivation of bacteria and hiv in buffy-coat-derived platelet concentrates under conditions that preserve in vitro platelet function. | a photochemical process has been tested for the inactivation of viruses and bacteria in buffy-coat derived platelet concentrates (bc pcs). | 2000 | 10895093 |
| growth of bacillus cereus on solid media as affected by agar, sodium chloride, and potassium sorbate. | the effect of two independent variables: microstructure, as modified by the agar content (1.0, 4.0, 7.0%), and water activity (a(w)), as modified by the nacl content (0.5, 2.5, 4.5%), in the absence or in the presence of potassium sorbate (0.0; 2,000 ppm) on bacillus cereus growth on solid media was studied. the time to visible growth (tvg) and the radial growth rate (rgr) of colonies were evaluated. tvg was not affected by microstructure and k-sorbate, although when a(w) was reduced, tvg tended ... | 2000 | 10914662 |
| investigating expression systems for the stable large-scale production of recombinant l-leucine-dehydrogenase from bacillus cereus in escherichia coli. | the established escherichia coli expression vectors ptrc99a, pkk223-3, ppllambda, pask75, pra95, and pra96, which differ in copy number, mode of induction, selection marker, and use of par sequences for stabilization, were investigated for the stable expression of recombinant l-leucine dehydrogenase from bacillus cereus with a view to large-scale production. best results were achieved with piet98, a runaway-replication system derived from pra96. expression of l-leucine dehydrogenase was controll ... | 2000 | 10919324 |
| molecular cloning, overproduction and characterization of the bacillus cereus imp dehydrogenase. | the gene of imp dehydrogenase of bacillus cereus ts-4, a temperature-sensitive mutant of b. cereus jcm 2152, was subcloned and its sequence was analyzed. a b. cereus ts-4 dna fragment of 2,065 bp containing the entire impdh gene and flanking regions was sequenced. the fragment contained an open reading frame of 1,527 bp encoding 509 amino acids with a calculated molecular mass of 55,390 da. the impdh sequence of jcm 2152 was also analyzed by ta cloning using pcr products amplified with primers f ... | 2000 | 10923792 |
| [ocular trauma in a 35 year old male]. | 2000 | 10932399 | |
| hazard analysis and critical control points of weaning foods. | hazards and critical control points were identified during the preparation, feeding and storage of weaning foods fed to the children (6-24 months) belonging to low income group (lig) families using survey methods, spot and indepth observations and microbial analysis. high microbial counts were obtained for the weaning food samples procured from the families that were rated as poor for both personal hygiene and environmental sanitation. food samples that were held at ambient temperature (25-35 de ... | 2000 | 10932959 |
| structural effects of the active site mutation cysteine to serine in bacillus cereus zinc-beta-lactamase. | beta-lactamases are involved in bacterial resistance. members of the metallo-enzyme class are now found in many pathogenic bacteria and are becoming thus of major clinical importance. despite the availability of zn-beta-lactamase x-ray structures their mechanism of action is still unclear. one puzzling observation is the presence of one or two zincs in the active site. to aid in assessing the role of zinc content in beta-lactam hydrolysis, the replacement by ser of the zinc-liganding residue cys ... | 2000 | 10933508 |
| recombinant acylheptapeptide lichenysin: high level of production by bacillus subtilis cells. | peptide synthetases are multi-domain proteins that catalyze the assembly, from amino acids and amino acid derivatives, of peptides and lipopeptides, some of which exhibit activities (pharmaceutical, surfactant, etc.) of considerable biotechnological importance. although there is substantial interest in the generation of greater peptide diversity, in order to create new biotechnologically interesting products, attempts reported so far to exchange amino acid-activating minimal modules between enzy ... | 2000 | 10939247 |
| biological variability and exposure assessment. | predictive models are now commonly used for exposure assessment, with growth parameters defined for each microbial species. in this study, we tried to take into account microbial growth variability among strains of a single species. bacillus cereus in pasteurized milk was chosen to illustrate the influence of the biological variability on the outcome of exposure assessment. each parameter of the exposure assessment (growth parameters, shelf-life conditions) was characterized by a probability dis ... | 2000 | 10939270 |
| cytotoxic cardenolides and antibacterial terpenoids from crossopetalum gaumeri. | from the methanol extract of the roots of (crossopetalum gaumeri, four new highly cytotoxic cardenolides, securigenin-3beta-o-beta-6-deoxyguloside (2), 19-hydroxy-sarmentogenin-3beta-o-beta-6-deoxyguloside (4), sarmentogenin-3beta-o-[alpha-allosyl-(1-->4)-beta-6-deoxy alloside] (5), and securigenin-3beta-o-[alpha-allosyl-(1-->4)-beta-6-deoxyal loside] (6) were isolated. the dichloromethane extract afforded the new diterpene 3,15-dihydroxy-18-norabieta-3,8,11,13-tetraene (7) as well as the new tr ... | 2000 | 10939358 |
| evidence for contribution of tripartite hemolysin bl, phosphatidylcholine-preferring phospholipase c, and collagenase to virulence of bacillus cereus endophthalmitis. | bacillus cereus causes a highly fulminant endophthalmitis which usually results in blindness. we previously concluded that hemolysin bl (hbl), a tripartite necrotizing pore-forming toxin, is a probable endophthalmitis virulence factor because it is highly toxic to retinal tissue in vitro and in vivo. we also determined that b. cereus produces additional retinal toxins that might contribute to virulence. here we fractionated crude b. cereus culture supernatant by anion-exchange chromatography and ... | 2000 | 10948154 |
| refractory bacillus cereus infection in a neonate. | bacillus cereus is a gram-positive aerobic or facultatively anaerobic spore-forming rod, which usually causes food poisoning. its recognition as a pathogen in neonates has increased over the past two decades. the clinical course of a neonate (gestation 24 weeks) with b. cereus infection refractory to therapy is described. death occurred after withdrawal of support following persistently positive blood and bone marrow cultures despite therapy with vancomycin, gentamicin, imipenum, clindamycin, ci ... | 2000 | 10954966 |
| the crystal structure of bacillus cereus phosphonoacetaldehyde hydrolase: insight into catalysis of phosphorus bond cleavage and catalytic diversification within the had enzyme superfamily. | phosphonoacetaldehyde hydrolase (phosphonatase) catalyzes the hydrolysis of phosphonoacetaldehyde to acetaldehyde and phosphate using mg(ii) as cofactor. the reaction proceeds via a novel bicovalent catalytic mechanism in which an active-site nucleophile abstracts the phosphoryl group from the schiff-base intermediate formed from lys53 and phosphonoacetaldehyde. in this study, the x-ray crystal structure of the bacillus cereus phosphonatase homodimer complexed with the phosphate (product) analog ... | 2000 | 10956028 |
| prevalence and expression of enterotoxins in bacillus cereus and other bacillus spp., a literature review. | members of the bacillus genus are ubiquitous soil microorganisms and are generally considered harmless contaminants. however, a few species are known toxin producers, including the foodborne pathogen, b. cereus. this species produces two distinct types of foodborne illness, the emetic (vomit-inducing) syndrome, associated with consumption of toxin in cooked rice dishes, and the diarrheal illness seen occasionally following consumption of contaminated meats, sauces, and certain dairy products. in ... | 2000 | 10959569 |
| a novel class of zinc-binding inhibitors for the phosphatidylcholine-preferring phospholipase c from bacillus cereus. | the phospholipase c (plc) isozymes catalyze the hydrolysis of phospholipids to provide diacylglycerol (dag) and a phosphorylated headgroup. because dag has been implicated in cellular signal transduction cascades in mammalian systems, there has been considerable interest in the development of inhibitors of these enzymes. toward this end, we have discovered that the cyclic n,n'-dihydroxyureas 6-10 inhibit the phosphatidylcholine preferring plc from bacillus cereus (plcbc). this class of inhibitor ... | 2000 | 10959851 |
| synthesis and antibacterial activity of new arylamido derivatives of 6 beta-aminopenicillanic, 7 beta-aminocephalosporanic and 7 beta-aminodesacetoxycephalosporanic acids. | new semisynthetic penicillins and cephalosporins have been synthesized by acylation of 6 beta-aminopenicillanic, 7 beta-aminocephalosporanic and 7 beta-aminodesacetoxycephalosporanic acids with ortho-substituted aromatic acids, using the method of mixed anhydrides. the chemical structures of the compounds obtained were confirmed by elemental analysis and by ir- and 1h-nmr spectra. antibacterial activities of the compounds were determined by the macrodilution susceptibility test in brain-heart in ... | 2000 | 10965424 |
| rapid characterization of spores of bacillus cereus group bacteria by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. | matrix-assisted laser desorption-ionization (maldi) time-of-flight mass spectrometry was used to characterize the spores of 14 microorganisms of the bacillus cereus group. this group includes the four bacillus species b. anthracis, b. cereus, b. mycoides, and b. thuringiensis. maldi mass spectra obtained from whole bacterial spores showed many similarities between the species, except for b. mycoides. at the same time, unique mass spectra could be obtained for the different b. cereus and b. thuri ... | 2000 | 10966397 |
| antibacterial activity of water and acetone extracts of the roots of euclea natalensis. | water and acetone extracts of the roots of euclea natalensis a.dc. were investigated for their in vitro antibacterial properties. the gram-positive bacteria tested appeared to be more susceptible to the extracts than the gram-negative bacteria. the water and acetone extracts inhibited the growth of bacillus cereus, bacillus pumilus, bacillus subtilis, micrococcus kristinae and staphylococcus aureus at concentrations ranging between 0.1 and 6.0 mg/ml. the water extract did not exert any inhibitor ... | 2000 | 10967488 |
| characterization and mechanism of action of cerein 7, a bacteriocin produced by bacillus cereus bc7. | cerein 7 is a peptidic antibiotic produced by bacillus cereus bc7 (cect 5148) at the end of exponential growth but before sporulation onset. cerein 7 has a broad spectrum of antibacterial activity against gram-positive bacteria, but it is inactive against gram-negative bacteria. the sequence of its amino-terminal end and its characteristics of hydrophobicity and molecular mass make cerein 7 unique among the bacteriocins produced by the soil bacterium b. cereus. in this paper a further characteri ... | 2000 | 10971770 |
| pulsed electric field inactivation of diarrhoeagenic bacillus cereus through irreversible electroporation. | the physical effects of high-intensity pulsed electric fields (pef) on the inactivation of diarrhoeagenic bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (tem). the levels of pef-induced microbial cell death were determined by enumeration on tryptone soy yeast extract agar and bacillus cereus-selective agar plates. following exposure to lethal levels of pef, tem investigation revealed irreversible cell membrane rupture at a number of locati ... | 2000 | 10972710 |
| selective amplification of bacterial rna: use of a dna primer containing mismatched bases near its 3' terminus to reduce false-positive signals. | a reverse transcription pcr (rt-pcr) method designed to reduce false-positive results due to the co-amplification of contaminating genomic dna is reported. feasibility of the method was evaluated using 16s rrna sequences specific to bacillus cereus. a dna oligonucleotide primer, consisting of 22-bases containing three consecutive mismatched bases near its 3' terminus (primer b16rt), was used for reverse transcription and in subsequent cdna amplification. specific rrna was reverse transcribed at ... | 2000 | 10972726 |
| fatal bacillus cereus bacteremia in a patient with diabetes. | this report describes a fatal case of bacillus cereus septicemia in a patient with uncontrolled diabetes and re-emphasizes the potential seriousness of bacillus infections in patients with compromised immune function. | 2000 | 10976178 |
| microbiological quality of reconstituted enteral formulations used in hospitals. | contamination of enteral feeds may occur during preparation, storage, decanting, and administration to patients. the aim of this study was to investigate the microbiological quality of reconstituted enteral feeds, residual feeds from feed delivery systems, and the water used to reconstitute powdered feeds in hospital. hazard analysis critical control points (haccp) system was implemented to control microbiological contamination of the enteral feeding formulations. before the implementation of th ... | 2000 | 10978852 |
| common occurrence of enterotoxin genes and enterotoxicity in bacillus thuringiensis. | seventy-four strains of bacillus thuringiensis thuringiensis representing 24 serovars were examined for the presence of three enterotoxin genes/operons; the non-haemolytic enterotoxin nhe, the haemolytic enterotoxin hbl and the bacillus cereus toxin bcet using polymerase chain reaction. the nhebc genes were found in all strains examined, the hblcd genes in 65 of the 74 strains and bcet in 63 strains. there was little consistency of the distribution of enterotoxin loci among strains of the same s ... | 2000 | 10981706 |
| stereocontrolled synthesis of a sphingomyelin methylene analogue as a sphingomyelinase inhibitor. | [reaction: see text]efficient synthesis of a sphingomyelin methylene analogue, which was designed as a sphingomyelinase inhibitor, was stereoselectively achieved. the hofmann rearrangement of the alpha-hydroxyethyl-beta-hydroxy amide 4 followed by the intramolecular oxazolidinone ring formation was one of the key steps. | 2000 | 10990413 |
| new antibacterial metabolites from the cyanobacterium nostoc commune(eawag 122b). | two new compounds, a diterpenoid and an anthraquinone, as well as an indane derivative, which is reported as a natural product for the first time, have been isolated from the cells of the cultured cyanobacterium nostoc commune (eawag 122b) by means of bioguided isolation. the structures were determined by spectroscopic methods, mainly nmr, infrared spectroscopy, and mass spectrometry. all isolates exhibit antibacterial activity. | 2000 | 11000038 |
| application of 2 microbioassays for evaluating the pollution present in the xochimilco and lerma-santiago basins]. | pollution due to urban-agricultural and urban-industrial activities, on the enzymatic activity of two microorganisms was evaluated. the zones under study are located in the caltongo "embarcadero", in xochimilco, d.f., and the basin of lerma-santiago river, state of mexico. nine and ten stations were established, respectively. samples of water and sediment were taken, in order to determine their ph, salinity, organic matter, as well as the toxic effect produced on escherichia coli beta-galactosid ... | 1999 | 11001710 |
| phosphatidylcholine-specific phospholipase c-mediated induction of phospholipase d activity in fas-expressing murine cells. | we have previously reported that fas cross-linking resulted in the activation of phosphatidylcholine-specific phospholipase c (pc-plc) and the subsequent activation of protein kinase c (pkc) and phospholipase d (pld) in a20 cells. in an attempt to correlate the existence of pc-plc activity and activation of pld by fas activation among various fas-expressing murine cell lines, we have investigated the effect of anti-fas monoclonal antibody on pc-plc and pld activities in a20, p388d1 and yac-1 cel ... | 2000 | 11007187 |
| research on factors allowing a risk assessment of spore-forming pathogenic bacteria in cooked chilled foods containing vegetables: a fair collaborative project. | vegetables are frequent ingredients of cooked chilled foods and are frequently contaminated with spore-forming bacteria (sfb). therefore, risk assessment studies have been carried out, including the following: hazard identification and characterisation--from an extensive literature review and expertise of the participants, b. cereus and c. botulinum were identified as the main hazards; exposure assessment--consisting of determination of the prevalence of hazardous sfb in cooked chilled foods con ... | 2000 | 11016602 |
| new developments in chromogenic and fluorogenic culture media. | this review describes some recent developments in chromogenic and fluorogenic culture media in microbiological diagnostic. the detection of beta-d-glucuronidase (gud) activity for enumeration of escherichia coli is well known. e. coli o157:h7 strains are usually gud-negative and do not ferment sorbitol. these characteristics are used in selective media for these organisms and new chromogenic media are available. some of the new chromogenic media make the salmonella diagnostic easier and faster. ... | 2000 | 11016610 |
| properties of bacillus cereus and other bacilli contaminating biomaterial-based industrial processes. | this paper is an overview on bacilli in industrial processes, with focus on food grade paper and paperboard production. paperboards mainly contain sporeforming bacteria belonging to the genera bacillus, paenibacillus and brevibacillus, usually found in quantities from < 50 to 250 cfu g(-1) homogenized paperboard. of those frequently found, bacillus cereus group, b. licheniformis, b. subtilis and brevibacillus brevis are important for food hygiene because of their hydrolytic activities on food co ... | 2000 | 11016612 |
| structural model requirements to describe microbial inactivation during a mild heat treatment. | the classical concept of d and z values, established for sterilisation processes, is unable to deal with the typical non-loglinear behaviour of survivor curves occurring during the mild heat treatment of sous vide or cook-chill food products. structural model requirements are formulated, eliminating immediately some candidate model types. promising modelling approaches are thoroughly analysed and, if applicable, adapted to the specific needs: two models developed by casolari (1988), the inactiva ... | 2000 | 11020040 |
| comparison of pcr-rflp, ribotyping and eric-pcr for typing bacillus anthracis and bacillus cereus strains. | pcr-rflp analysis of the vrra gene and cerab gene was used to investigate the genomic diversity in 21 strains of bacillus anthracis and 28 strains of bacillus cereus, and was compared with results obtained by ribotyping and enterobacterial repetitive intergenic consensus-pcr (eric-pcr) analysis. vrra-typing divided the b. anthracis into four groups. except for one pasteur vaccine strain, the vrra pcr-rflp profiles of the b. anthracis were separated into three groups, which were different from th ... | 2000 | 11021577 |
| soybean vegetable protein (tofu) preserved with high pressure. | tofu is a soybean vegetable protein that asians have long consumed; its intake is increasing in other countries. tofu was purchased at a local shop. the tofu samples were already preserved in plastic bags subjected to vacuum and storage (5 c). tofu samples were subjected to high pressure (hp) of 400 mpa at 5 c for 5, 30, and 45 mm. microbial analysis, sensorial evaluation, and structure were determined. hp treatment in tofu reduces the microbial population. most of the microorganisms found in th ... | 2000 | 11032484 |
| characterization of the chemotaxis fli y and che a genes in bacillus cereus. | this paper describes the first identification of chemotaxis genes in bacillus cereus. we sequenced and studied the genomic organization and the expression of the chea and fliy genes in two different b. cereus strains, atcc 14579 and atcc 10987. while chea encodes a highly conserved protein acting as the main regulator of the chemotactic response in flagellated eubacteria, fliy, which has been previously described only in b. subtilis, is one of the three genes encoding proteins of the flagellar s ... | 2000 | 11034287 |
| involvement of a pasteurizer in the contamination of milk by bacillus cereus in a commercial dairy plant. | 2000 | 11037242 | |
| antimicrobial effect of rosemary extracts. | a rosemary extract commercially exploited (oxy'less) as an antioxidant of lipids in foods was dissolved in ethanol (100 mg/ml), and the solution was tested against foodborne microorganisms. for gram-positive bacteria, the mic of the ethanolic solution was 1% for leuconostoc mesenteroides, 0.5% for listeria monocytogenes, 0.5% for staphylococcus aureus, 0.13% for streptococcus mutans, and 0.06% for bacillus cereus. it slowed the growth of penicillium roquefortii and botrytis cinerea. up to 1% of ... | 2000 | 11041135 |
| human cell exposure assays of bacillus thuringiensis commercial insecticides: production of bacillus cereus-like cytolytic effects from outgrowth of spores. | most contemporary bioinsecticides are derived from scaled-up cultures of bacillus thuringiensis subspecies israelensis (bti) and kurstaki (btk), whose particulate fractions contain mostly b. thuringiensis spores (> 10(12)/l) and proteinaceous aggregates, including crystal-like parasporal inclusion bodies (pib). based on concerns over relatedness to b. cereus-group pathogens, we conducted extensive testing of b. thuringiensis (bt) products and their subfractions using seven human cell types. the ... | 2000 | 11049810 |
| outbreak of bacillus cereus infections in a neonatal intensive care unit traced to balloons used in manual ventilation. | in 1998, an outbreak of systemic infections caused by bacillus cereus occurred in the neonatal intensive care unit of the university hospital vrije universiteit, amsterdam, the netherlands. three neonates developed sepsis with positive blood cultures. one neonate died, and the other two neonates recovered. an environmental survey, a prospective surveillance study of neonates, and a case control study were performed, in combination with molecular typing, in order to identify potential sources and ... | 2000 | 11060080 |
| kinetic steps for the hydrolysis of sphingomyelin by bacillus cereus sphingomyelinase in lipid monolayers. | the sphingomyelinase (sphmase) activity degrading sphingomyelin (sphm) monolayers shows a slow-reaction latency period before exhibiting constant rate catalysis. these two kinetic regions are regulated independently by the lateral surface pressure and by lipids that are biomodulators of cell function such as ceramide, glycosphingolipids, fatty acids, and lysophospholipids. knowledge of the interfacial adsorption of sphmase, precatalytic activation, initiation of effective catalysis, and the corr ... | 2000 | 11060353 |
| the plcr regulon is involved in the opportunistic properties of bacillus thuringiensis and bacillus cereus in mice and insects. | bacillus thuringiensis has been widely used for 40 years as a safe biopesticide for controlling agricultural pests and mosquitoes because it produces insecticidal crystal proteins. however, spores have also been shown to contribute to overall entomopathogenicity. here, the opportunistic properties of acrystalliferous b. thuringiensis cry(-) and bacillus cereus strains were investigated in an insect species, galleria mellonella, and in a mammal, balb/c mice. in both animal models, the pathogenici ... | 2000 | 11065361 |
| bacillus cereus produces most emetic toxin at lower temperatures. | seven emetic toxin-producing strains of bacillus cereus were examined for toxin production in skim milk medium at incubation temperatures ranging from 10 to 50 degrees c. minimum and maximum growth temperatures were found to be 12 and 46 degrees c, respectively. at 12 and 15 degrees c, levels of toxin production were significantly higher (p < 0.01) than that observed at 30 degrees c, while no toxin was produced above 37 degrees c. increased levels of sporulation were observed at increased temper ... | 2000 | 11069642 |
| a new cytotoxin from bacillus cereus that may cause necrotic enteritis. | a cytotoxin (cytk) has been isolated from a bacillus cereus strain that caused a severe food poisoning outbreak killing three people. a protein of 34 kda was highly cytotoxic, and the addition of other secreted proteins gave no synergistic effect. cytk was also necrotic and haemolytic. no known b. cereus enterotoxins were produced by this strain. a dna sequence from 1.8 kb upstream to 0.2 kb downstream of the toxin gene was sequenced. the deduced amino acid sequence of the toxin showed similarit ... | 2000 | 11069652 |
| ceramide mass analysis by normal-phase high-performance liquid chromatography. | 2000 | 11070859 | |
| bacillus cereus endophthalmitis secondary to self-inflicted periocular injection. | 2000 | 11074820 | |
| inhibition of bacillus cereus atcc 14579 by plantaricin ug1 in vitro and in food. | the inhibition of bacillus cereus atcc 14579 viable growth by lactobacillus plantarum ug1 bacteriocin (plantaricin ug1) in vitro and in food (pasteurized milk and minced meat) was studied. the inhibitory effect against b. cereus food-borne pathogen noticed in this study was due to plantaricin ug1, but not due to lactic acid produced by the l. plantarum ug1 culture. plataricin ug1 negative clone did not affect viable growth of b. cereus in both broth and meat or pasteurized milk. the inhibitory e ... | 2000 | 11075381 |
| microbiological hazard identification and exposure assessment of street food vending in johannesburg, south africa. | one hundred and thirty-two samples of beef, chicken, salad and gravy were collected from two street vendors over eleven replicate surveys to assess microbiological safety and quality. for each food type samples were collected during preparation and holding. dish water was also collected and food preparation surfaces swabbed during preparation and display. standard methods were used to determine aerobic plate counts, enterobacteriaceae counts, coliform counts and spore counts. six hundred and sev ... | 2000 | 11078164 |
| efficacy of electrolyzed oxidizing (eo) and chemically modified water on different types of foodborne pathogens. | this study was undertaken to evaluate the efficacy of electrolyzed oxidizing (eo) and chemically modified water with properties similar to the eo water for inactivation of different types of foodborne pathogens (escherichia coli o157:h7, listeria monocytogenes and bacillus cereus). a five-strain cocktail of each microorganism was exposed to deionized water (control), eo water and chemically modified water. to evaluate the effect of individual properties (ph, oxidation-reduction potential (orp) a ... | 2000 | 11078171 |
| bacillus cereus group strains, their hemolysin bl activity, and their detection in foods using a 16s rna and hemolysin bl gene-targeted multiplex polymerase chain reaction system. | hemolysin bl (hbl) is a major virulence factor for bacillus cereus group strains. it is also a target enterotoxin for the most commonly used b. cereus detection kit, i.e., the b. cereus enterotoxin (diarrheal type) reversed passive latex agglutination (bcet-rpla) test kit. a survey of the hbl activities and the cytotoxicities to the chinese hamster ovary (cho) cells for the b. cereus group strains, however, showed that although only part of the b. cereus group strains are hbl active, all strains ... | 2000 | 11079690 |
| bacterial spore inhibition and inactivation in foods by pressure, chemical preservatives, and mild heat. | sucrose laurates, sucrose palmitate, sucrose stearates, and monolaurin (lauricidin) were evaluated for inhibitory effects against spores of bacillus sp., clostridium sporogenes pa3679, and alicyclobacillus sp. in a model agar system. the combined treatment of sucrose laurate, high hydrostatic pressure, and mild heat was evaluated on spores of bacillus and alicyclobacillus in foods. the minimum inhibitory concentrations of the sucrose esters were higher than that of lauricidin for all spores test ... | 2000 | 11079691 |
| the synergistic effect of excimer and low-pressure mercury lamps on the disinfection of flowing water. | microorganisms in flowing water were disinfected by uv radiation from two excimer (excited dimer) lamps (emitting at 172 and 222 nm) in combination with two low-pressure mercury lamps (emitting at 254 nm). synergies were investigated among the three types of radiation in the treatment of water spiked in turn with escherichia coli, listeria innocua, shewanella putrefaciens, and spores of bacillus subtilis and bacillus cereus. synergy was demonstrated between radiations at 222 and 254 nm in the tr ... | 2000 | 11079695 |