Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the capability of catabolic utilization of n-acetylneuraminic acid, a sialic acid, is essential for vibrio vulnificus pathogenesis. | n-acetylneuraminic acid (neu5ac, sialic acid) could provide a good substrate for enteropathogenic bacteria in the intestine, when the bacteria invade and colonize in human gut. in order to analyze the role of neu5ac catabolism in vibrio vulnificus pathogenesis, a mutant with disruption of the nana gene encoding neu5ac lyase was constructed by allelic exchanges. the nana mutant was not able to utilize neu5ac as a sole carbon source and revealed an altered colony morphotype with reduced opacity in ... | 2009 | 19487477 |
| [effects of antimicrobial agents on the toll like receptors and myeloid differentiation protein-2 in liver tissue of alcohol-induced liver disease: experiment with rats]. | to investigate the effects of antimicrobial agents on the toll like receptors (tlrs) and myeloid differentiation protein (md)-2 in liver tissue of alcohol-induced liver disease with vibrio vulnificus (vv) sepsis. | 2009 | 19489280 |
| user friendly cloning coupled with chitin-based natural transformation enables rapid mutagenesis of vibrio vulnificus. | vibrio vulnificus is a bacterial contaminant of shellfish and causes highly lethal sepsis and destructive wound infections. a definitive identification of virulence factors using the molecular version of koch's postulates has been hindered because of difficulties in performing molecular genetic analysis of this opportunistic pathogen. for example, conjugation is required to introduce plasmid dna, and allelic exchange suicide vectors that rely on sucrose sensitivity for counterselection are not e ... | 2009 | 19502446 |
| septic shock from penetrating leg injury with vibrio vulnificus infection. | 2009 | 19527215 | |
| benthic ecology of vibrio spp. and pathogenic vibrio species in a coastal mediterranean environment (la spezia gulf, italy). | we carried out a 16-month in situ study to investigate the ecology of vibrio spp. and pathogenic vibrio species in coastal sediments of the mediterranean sea, employing multiple-regression analysis to reveal the major environmental factors controlling their occurrence in the benthic environment. in addition, association between vibrios and sediment-inhabiting meiofauna, which is a major component of benthic ecosystems, was investigated. culturable and total vibrio spp. estimates by most-probable ... | 2009 | 19543938 |
| infections in patients with aplastic anemia. | infection is a major cause of death in patients with aplastic anemia (aa). there are differences between the immunocompromised state of a patient with aa and the patient who is neutropenic due to chemotherapy and this leads to a difference in the infections that they incur. prolonged neutropenia is one of the largest risk factors for the development of infections with the invasive mycoses and bacteria. recovery from neutropenia is directly related to survival, and supportive care plays a large r ... | 2009 | 19549579 |
| comparative evolutionary analysis of the major structural subunit of vibrio vulnificus type iv pili. | type iv pili contribute to virulence in vibrio vulnificus, the bacterium responsible for the majority of fatal seafood-related infections. here, we performed within- and between-species evolutionary analysis of the gene that encodes the major structural subunit of the pilus, pila, by comparing it with pild and gyrb, the genes encoding the type iv prepilin peptidase and beta subunit of dna gyrase, respectively. although the diversity in pild and gyrb is similar to each other and likely to have ac ... | 2009 | 19556347 |
| roles of rseb, sigmae, and degp in virulence and phase variation of colony morphotype of vibrio vulnificus. | vibrio vulnificus is an estuarine bacterium capable of causing serious and often fatal wound infections and primary septicemia. we used alkaline phosphatase insertion mutagenesis to identify genes necessary for the virulence of this pathogen. one mutant had an in-frame fusion of 'phoa to the gene encoding rseb, a periplasmic negative regulator of the alternative sigma factor sigma(e). sigma(e) controls an extensive regulon involved in responding to cell envelope stresses. colonies of the rseb mu ... | 2009 | 19564391 |
| incidence of vibrio vulnificus in estuarine waters of the south texas coastal bend region. | to determine the occurrence of the human pathogen, vibrio vulnificus, in south texas coastal waters. | 2009 | 19566724 |
| iron differentially regulates gene expression and extracellular secretion of vibrio vulnificus cytolysin-hemolysin. | in a previous study, we showed that vibrio vulnificus is a ferrophilic bacterium that requires high levels of available iron for growth. in the present study, we show that iron stimulates, in an unusual manner, the production of cytolysin-hemolysin (vvha), the most potent exotoxin produced by v. vulnificus. the vvha gene possesses a putative ferric uptake regulator (fur)-binding box in the regulatory region, andvvha transcription was repressed by iron and de-repressed by fur mutation. however, e ... | 2009 | 19586415 |
| septic arthritis as the initial manifestation of fatal vibrio vulnificus septicemia in a patient with thalassemia and iron overload. | vibrio vulnificus infection is an uncommon but potentially fatal disease in children such that prompt recognition has prognostic implications. we describe here the case of a 9-year-old female with thalassemia and iron overload who presented with septic arthritis as an atypical initial manifestation of fatal v. vulnificus septicemia. this report underscores the possibility of septic arthritis as an early manifestation of v. vulnificus septicemia. pediatricians should be alert to this extremely in ... | 2009 | 19591224 |
| integrase-directed recovery of functional genes from genomic libraries. | large population sizes, rapid growth and 3.8 billion years of evolution firmly establish microorganisms as a major source of the planet's biological and genetic diversity. however, up to 99% of the microorganisms in a given environment cannot be cultured. culture-independent methods that directly access the genetic potential of an environmental sample can unveil new proteins with diverse functions, but the sequencing of random dna can generate enormous amounts of extraneous data. integrons are r ... | 2009 | 19596808 |
| the fragment structure of a putative hsdr subunit of a type i restriction enzyme from vibrio vulnificus yj016: implications for dna restriction and translocation activity. | among four types of bacterial restriction enzymes that cleave a foreign dna depending on its methylation status, type i enzymes composed of three subunits are interesting because of their unique dna cleavage and translocation mechanisms performed by the restriction subunit (hsdr). the elucidated n-terminal fragment structure of a putative hsdr subunit from vibrio vulnificus yj016 reveals three globular domains. the nucleolytic core within an n-terminal nuclease domain (ntd) is composed of one ba ... | 2009 | 19625490 |
| in vitro and in vivo antimicrobial activity of water-soluble chitosan oligosaccharides against vibrio vulnificus. | vibrio vulnificus is a gram-negative bacterium that induces severely rapid pathological progress. in this study, we evaluated the antibacterial activity of two water-soluble chitosan oligosaccharides, cos a (mw, 10,000 da) and cos b (mw, 1,000 da), from 90-95% deacetylated chitosan, against v. vulnificus in vitro and in vivo. treatment with cos a resulted in significantly higher suppressive effects on the growth of v. vulnificus than treatment with cos b. the growth of v. vulnificus was inhibite ... | 2009 | 19639224 |
| calcium is essential for the major pseudopilin in the type 2 secretion system. | the pseudopilus is a key feature of the type 2 secretion system (t2ss) and is made up of multiple pseudopilins that are similar in fold to the type 4 pilins. however, pilins have disulfide bridges, whereas the major pseudopilins of t2ss do not. a key question is therefore how the pseudopilins, and in particular, the most abundant major pseudopilin, gspg, obtain sufficient stability to perform their function. crystal structures of vibrio cholerae, vibrio vulnificus, and enterohemorrhagic escheric ... | 2009 | 19640838 |
| phase variation, capsular polysaccharide, pilus and flagella contribute to uptake of vibrio vulnificus by the eastern oyster (crassostrea virginica). | vibrio vulnificus infections are associated with raw oyster consumption, and disease reservoirs are determined by the ability of this bacterium to infect and persist in oysters. surface structures, such as capsular polysaccharide (cps), pili and flagella, function as virulence factors in mouse infection models. furthermore, virulence is related to phase variation in colony morphology, which reflects cps expression and includes opaque (encapsulated, virulent), translucent (reduced encapsulation, ... | 2009 | 19689704 |
| structure of a polysaccharide from the lipopolysaccharides of vibrio vulnificus strains cect 5198 and s3-i2-36, which is remarkably similar to the o-polysaccharide of pseudoalteromonas rubra atcc 29570. | high-molecular-mass polysaccharides were released by mild acid degradation of the lipopolysaccharides of two wild-type vibrio vulnificus strain, a flagellated motile strain cect 5198 and a non-flagellated non-motile strain s3-i2-36. studies by sugar analysis and partial acid hydrolysis along with (1)h and (13)c nmr spectroscopies showed that the polysaccharides from both strains have the same trisaccharide repeating unit of the following structure: -->4)-beta-d-glcpnac3nacylan-(1-->4)-alpha-l-ga ... | 2009 | 19695563 |
| rigorous vibrio vulnificus soft tissue infection of the lower leg in a renal transplant patient managed by vacuum therapy and autologous growth factors. | vibrio vulnificus is a gram-negative marine bacterium that grows well in coastal waters. it is an opportunistic pathogen that can cause serious life-threatening infections in patients with certain health conditions. vibrio-induced wound infections in immunosuppressed patients are difficult to treat because the healing process may be significantly delayed. reconstructive surgery may not be successful in early treatment as skin grafts are likely to fail, and there may be increased morbidity of don ... | 2009 | 19706229 |
| role of catalase and oxyr in the viable but nonculturable state of vibrio vulnificus. | vibrio vulnificus has proven difficult to culture from water or shellfish during winter months, which is attributed to the viable but nonculturable (vbnc) state. because reactive oxygen species were found to be involved in the low temperature-induced entrance of v. vulnificus into this state, we generated an oxyr mutant which lacks catalase activity. this strain is nonculturable on solid media even at ambient temperature, due to the presence of h(2)o(2) in such media. low temperature incubation ... | 2004 | 19712354 |
| ecology of vibrio vulnificus and vibrio parahaemolyticus in brackish environments of the sada river in shimane prefecture, japan. | while there are several studies on the ecology of vibrio vulnificus and vibrio parahaemolyticus in estuarine water environments around the world, there is little information on the distribution of both organisms during the cold-weather months. thus, we conducted a multi-year study on the ecology of both organisms in brackish environments of the sada river, a drainage canal flowing slowly into the japan sea from lake shinji in shimane prefecture, japan. water samples were collected twice a month ... | 2004 | 19712405 |
| seasonal shifts in population structure of vibrio vulnificus in an estuarine environment as revealed by partial 16s ribosomal dna sequencing. | abstract the partial sequence (600 bp) containing the most variable region of vibrio vulnificus 16s ribosomal dna (rdna) was determined for 208 randomly selected v. vulnificus strains isolated from galveston bay, tx, usa between june 2000 and june 2001. a comparative analysis of the determined partial 16s rdna sequences revealed the existence of two different partial 16s rdna sequences (type a and type b, 1.3% base substitutions) among the 208 v. vulnificus isolates. a higher proportion of 16s r ... | 2003 | 19719603 |
| growth and survival differences of vibrio vulnificus and vibrio parahaemolyticus strains during cold storage. | vibrio vulnificus and vibrio parahaemolyticus are the most common vibrio species associated with seafood illness in the united states. our study was conducted to determine if strain-to-strain differences exist in the growth and survival of 8 different v. vulnificus and v. parahaemolyticus strains at low temperatures. by day 10, v. vulnificus strain 515-4c2 had significantly higher counts (p < 0.05) (1.97 log cfu/g) compared with strains 3315, 1007, 29306 at 5 degrees c, which reached nondetectab ... | 2009 | 19723217 |
| occurrence of potentially pathogenic vibrios in final effluents of a wastewater treatment facility in a rural community of the eastern cape province of south africa. | we assessed the occurrence of vibrio pathogens in the final effluents of a rural wastewater treatment facility in the eastern cape province of south africa as free or plankton-associated (180 microm, 60 microm and 20 microm plankton sizes) entities using standard culture-based and molecular techniques. the free-living vibrio densities varied from 0 to 3.45 x 10(1) cfu ml(-1), while the plankton-associated vibrio densities vary with plankton sizes as follows: 180 microm (0-4.50 x 10(3) cfu ml(-1) ... | 2009 | 19732825 |
| discrimination of viable and dead vibrio vulnificus after refrigerated and frozen storage using ema, sodium deoxycholate and real-time pcr. | the effect of refrigerated and frozen storage on the viability of vibrio vulnificus was evaluated using cell suspensions (1x10(8) cfu/ml). ethidium bromide monoazide (ema) was utilized to selectively allow real-time (rti) pcr amplification of target dna from viable but not dead cells. bacterial survivors from the ema rti-pcr were evaluated by comparison with the plate count assay following different temperature exposures (-20 and 4 degrees c) every 24 h for 72 h. the log cfu values from the ema ... | 2009 | 19733199 |
| role of ntrc-regulated exopolysaccharides in the biofilm formation and pathogenic interaction of vibrio vulnificus. | vibrio vulnificus has been shown to require a global transcription factor, ntrc for mature biofilm development via controlling the biosyntheses of lipopolysaccharide and exopolysaccharide (eps). biofilm formation and eps production were dramatically increased in a medium including a tricarboxylic acid cycle-intermediate as a carbon source. these phenotypes required functional ntrc and were abolished by the addition of ammonium chloride. during the initial stage of biofilm formation, both express ... | 2009 | 19737353 |
| characterization of clinical and environmental types of vibrio vulnificus isolates from louisiana oysters. | vibrio vulnificus, a naturally occurring estuarine bacterium frequently transmitted to humans via raw oysters, is a leading cause of seafood-related deaths in the united states. although unique virulence markers have not been identified to date, multiple biomarkers have been used previously to associate strains with clinical or environmental types of v. vulnificus. in this study, we evaluated the usefulness of these biomarkers in characterizing 349 v. vulnificus oyster isolates by the presence/a ... | 2009 | 19743928 |
| epidemiologic study of vibrio vulnificus infections by using variable number tandem repeats. | a 3-year environmental and clinical vibrio vulnificus survey using simple-sequence repeats typing shows that v. vulnificus biotype 3 constitutes approximately 21% of the bacterium population in tested aquaculture ponds as opposed to approximately 86% of clinical cases. simple-sequence repeats proved to be a useful epidemiologic tool, providing information on the environmental source of the pathogen. | 2009 | 19751592 |
| ai-2 quorum-sensing inhibitors affect the starvation response and reduce virulence in several vibrio species, most likely by interfering with luxpq. | the increase of disease outbreaks caused by vibrio species in aquatic organisms as well as in humans, together with the emergence of antibiotic resistance in vibrio species, has led to a growing interest in alternative disease control measures. quorum sensing (qs) is a mechanism for regulating microbial gene expression in a cell density-dependent way. while there is good evidence for the involvement of auto-inducer 2 (ai-2)-based interspecies qs in the control of virulence in multiple vibrio spe ... | 2009 | 19778962 |
| effect of antimicrobial agents on the toll-like receptors and inflammatory cytokines in liver tissue of the alcohol-induced liver disease in rats with vibrio vulnificus sepsis. | septicemia and inflammation-mediated septic shock caused by vibrio vulnificus (vv) is strongly associated with chronic liver disease. this study examined the effects of antimicrobial therapy on expression of hepatic toll-like receptors and inflammatory cytokines in rats with alcohol-induced liver disease complicated by vv sepsis. | 2009 | 19781370 |
| correlation of mannitol fermentation with virulence-associated genotypic characteristics in vibrio vulnificus isolates from oysters and water samples in the gulf of mexico. | vibrio vulnificus strains (n = 469) isolated from the gulf of mexico oysters and waters over a period of 2 years were subjected to phenotypic and genotypic characterizations. of the strains that could be definitively genotyped (n = 465), 58% were classified as genotype a, 29% as genotype b, and 13% as genotype a/b by 16s rrna genotyping. when the same strain bank was characterized by virulence-correlated gene (vcg) typing, 65% were genotype e while 35% were genotype c. further analysis focusing ... | 2010 | 19785540 |
| knowledge of vibrio vulnificus infection among japanese patients with liver diseases: a prospective multicenter study. | vibrio vulnificus (v. vulnificus) is a seafood-borne infectious pathogen that can be lethal to humans. the infection has been correlated with pre-existing liver disease, particularly liver cirrhosis. awareness of v. vulnificus infection among japanese citizens is low, despite the increasing number of patients with hepatocellular carcinoma (hcc). the present study was conducted to assess the level of knowledge of patients with liver disease regarding v. vulnificus infection. | 2009 | 19789520 |
| evaluation of dna colony hybridization and real-time pcr for detection of vibrio parahaemolyticus and vibrio vulnificus in postharvest-processed oysters. | the applicability of real-time pcr was examined for detection of vibrios from postharvest-processed (php) oysters to allow for a more rapid assay and higher sample throughput than currently used. during june to october 2004, 68 php oyster samples were collected directly from php firms or from retail markets across the united states. php oysters were examined to determine the effectiveness of treatments in the reduction of vibrio levels and to compare the analytical methods utilized. the latter i ... | 2009 | 19833033 |
| survey of postharvest-processed oysters in the united states for levels of vibrio vulnificus and vibrio parahaemolyticus. | from june through october 2004, the u.s. food and drug administration collected oysters (61 samples) that had been subjected to postharvest processing (php) methods, including mild heat treatment, freezing, and high hydrostatic pressure, from processors and retail markets in various states to determine vibrio vulnificus and v. parahaemolyticus levels. presence in a 25-g sample and most probable number (mpn) using standard enrichment and selective isolation procedures were utilized. suspect colon ... | 2009 | 19833034 |
| tropical dermatology: marine and aquatic dermatology. | dermatoses caused by marine organisms are frequently seen in dermatology clinics worldwide. cutaneous injuries after exposure to marine environments include bacterial and fungal infections and lesions caused by aquatic plants and protists. some of these diseases are well known by dermatologists, such as vibrio vulnificus septicemia and erysipeloid, but others are uncommon, such as envenomation caused by ingestion or contact with certain dinoflagellates or cyanobacteria, which are associated with ... | 2009 | 19836641 |
| identification of the vibrio vulnificus ahpcl gene and its influence on survival under oxidative stress and virulence. | pathogens have evolved sophisticated mechanisms to survive oxidative stresses imposed by host defense systems, and the mechanisms are closely linked to their virulence. in the present study, ahpcl, a homologue of escherichia coli ahpc encoding a peroxiredoxin, was identified among the vibrio vulnificus genes specifically induced by exposure to h(2)o(2). in order to analyze the role of ahpcl in the pathogenesis of v. vulnificus, a mutant, in which the ahpcl gene was disrupted, was constructed by ... | 2009 | 19851736 |
| vibrio vulnificus infection: a case infected by contaminated fishing gear. | 2009 | 19878399 | |
| molecular cloning, characterization and expression analysis of a c-type lectin (ec-ctl) in orange-spotted grouper, epinephelus coioides. | c-type lectins play crucial roles in pathogen recognition, innate immunity, and cell-cell interactions. in this study, a new c-type lectin (ec-ctl) gene was cloned from grouper, epinephelus coioides by expressed sequence tag (est) and rapid amplification of cdna ends (race) pcr. the full-length cdna of ec-ctl was composed of 840 bp with a 651 bp open reading frame (orf) that encodes a 216-residue protein. the deduced amino acid sequence of ec-ctl possessed all conserved features crucial for the ... | 2010 | 19879363 |
| cadaverine is transported into vibrio vulnificus through its cadb in alkaline environment. | the exogenously-added cadaverine is effective in protecting vibrio vulnificus from methyl viologen (mv)-induced superoxide stress at ph 8.5. such a protective effect by cadaverine was not observed at ph 7.5. consistently, the accumulated level of intracellular cadaverine at ph 8.5 is approximately four times as much as that of the control cell at ph 7.5. cadaverine accumulation is not affected by mv. the protection of v. vulnificus by cadaverine from superoxide stress was abolished when cadb cod ... | 2009 | 19884768 |
| oligomerization is essential for apoptotic activity of vibrio vulnificus hemolysin. | vibrio vulnificus hemolysin (vvh), a pore forming toxin, is thought to be a virulence factor of this bacterium. it is well known that vvh induces apoptosis as well as cell lysis in susceptible target cells. although pore formation is an essential step in cell lysis, it is unknown whether this step is necessary for vvh-induced apoptosis. in this study, chinese hamster ovary (cho) cells were exposed to non-oligomerized mutant f334i, in which phenylalanine 334 was replaced by isoleucine. the rate o ... | 2009 | 19887751 |
| simultaneous differential detection of human pathogenic and nonpathogenic vibrio species using a multiplex pcr based on gyrb and pnta genes. | to develop a multiplex pcr targeting the gyrb and pnta genes for vibrio species differentiation. | 2010 | 19891709 |
| the aromatic ring of phenylalanine 334 is essential for oligomerization of vibrio vulnificus hemolysin. | vibrio vulnificus hemolysin (vvh) is thought to be a member of the cholesterol-dependent cytolysin (cdc) family of pore-forming toxins. to date, the structure-function relationships of cdcs produced by gram-negative bacteria remain largely unknown. we show here that the aromatic ring of phenylalanine residue conserved in vibrionaceae hemolysins is essential for oligomerization of vvh. we generated the vvh mutants; substituted phe 334 for ile (f334i), ala (f334a), tyr (f334y), or trp (f334w); and ... | 2010 | 19897654 |
| validation of a method for the detection of five species, serogroups, biotypes and virulence factors of vibrio by multiplex pcr in fish and seafood. | in this work a sequential multiplex pcr system was designed and validated for the detection of most frequent foodborne pathogen vibrio species in fish and seafood (vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio alginoliticus and vibrio mimicus). the method proposed functions in a hierarchical way, being composed of an end-point multiplex pcr to detect the presence of dna belonging to the studied species, followed by multiplex pcr and fragment analysis allowing the viability ... | 2010 | 19913702 |
| rtxa1-induced expression of the small gtpase rac2 plays a key role in the pathogenicity of vibrio vulnificus. | infection with the human pathogen vibrio vulnificus leads to the generation of reactive oxygen species (ros) via nad(p)h oxidase (nox) in host cells. in the present study, we employed mutant v. vulnificus strains to identify an essential virulence factor responsible for this ros generation. we found that repeats-in-toxin a1 (rtxa1) expressed by v. vulnificus acts via nox1 to induce significant ros generation in the intestine epithelial cells, which ultimately results in cell death. furthermore, ... | 2010 | 19919301 |
| american oyster, crassostrea virginica, expresses a potent antibacterial histone h2b protein. | an antibacterial protein was purified from acidified gill extract of a bivalve mollusk, the american oyster (crassostrea virginica). protein isolation was best accomplished by briefly boiling the tissues in a weak acetic acid solution. adding protease inhibitors while boiling did not have a major effect on activity recovery. in contrast, use of only protease inhibitors (without boiling) resulted in virtually no recovery of this activity. the amino acid sequence of this antibacterial protein was ... | 2010 | 19949827 |
| [development of taqman real-time pcr in detection of aeromonas hydrophila]. | to develop a taqman real-time pcr for the detection of aeromonas hydrophila. | 2009 | 19954074 |
| presence of pathogens and indicator microbes at a non-point source subtropical recreational marine beach. | swimming in ocean water, including ocean water at beaches not impacted by known point sources of pollution, is an increasing health concern. this study was an initial evaluation of the presence of indicator microbes and pathogens and the association among the indicator microbes, pathogens, and environmental conditions at a subtropical, recreational marine beach in south florida impacted by non-point sources of pollution. twelve water and eight sand samples were collected during four sampling eve ... | 2010 | 19966020 |
| a recombinant metalloprotease antigen of vibrio vulnificus elicits protective antibodies in a murine model. | to investigate whether vibrio vulnificus metalloprotease (vvpe) can induce the production of specific anti-vvpe antibody to confer effective protection against vibrio vulnificus infection and to evaluate the possibility of vvpe as a potential vaccine candidate against disease caused by v. vulnificus. | 2010 | 20002577 |
| evaluation of bactericidal activity of weakly acidic electrolyzed water (waew) against vibrio vulnificus and vibrio parahaemolyticus. | vibrio parahaemolyticus and vibriovulnificus cause severe foodborne illness in humans; thus, to reduce outbreaks of disease, it is clearly important to reduce food contamination by these pathogens. although electrolyzed oxidizing (eo) water has been reported to exhibit strong bactericidal activities against many pathogens, it has never been tested against v. vulnificus and v. parahaemolyticus. the purpose of this study was to evaluate the bactericidal activity of weakly acidic electrolyzed water ... | 2010 | 20004034 |
| thrombocytopenia and thrombosis in disseminated intravascular coagulation (dic). | disseminated intravascular coagulation (dic) is the physiologic result of pathologic overstimulation of the coagulation system. despite multiple triggers, a myriad of laboratory abnormalities, and a clinical presentation ranging from gross hemostatic failure to life-threatening thrombosis, or even both simultaneously, a simplified clinical approach augmented by a few readily available tests allows prompt identification of the process and elucidation of treatment opportunities. platelet counts in ... | 2009 | 20008204 |
| [vibrio vulnificus infection outside of the fish ponds in northern israel: acquisition in the "einot tzukim" springs near the dead sea]. | vibrio vulnificus is a gram-negative bacterium that may cause severe skin and systemic infection after exposure of open wounds to contaminated water, especially in patients with underlying disease such as immune-deficiency, iron overload or end stage liver or renal disease. the v. vulnificus infection has been reported in israel almost exclusively after exposure to tilapia fish cultivated in fresh water fish ponds in northern israel. the authors report the first case of v. vulnificus infection a ... | 2009 | 20027981 |
| [real-time pcr in rapid diagnosis of aeromonas hydrophila necrotizing soft tissue infections]. | we report a case of rapidly progressive necrotizing soft tissue infection and sepsis followed by a patient's death. we suspected vibrio vulnificus infection because the patient's underlying disease was cirrhosis and the course extremely rapid. no microbe had been detected at death. we extracted dna from a blood culture bottle. sybr green i real-time pcr was conducted but could not detect v. vulnificus vvh in the dna sample. aeromonas hydrophila was cultured and identified in blood and necrotized ... | 2009 | 20034324 |
| pilf polymorphism-based pcr to distinguish vibrio vulnificus strains potentially dangerous to public health. | vibrio vulnificus is a heterogeneous species that comprises strains virulent and avirulent for humans and fish, and it is grouped into three biotypes. in this report, we describe a pcr-based methodology that allows both the species identification and discrimination of those isolates that could be considered dangerous to public health. discrimination is based on the amplification of a variable region located within the gene pilf, which seems to be associated with potential human pathogenicity, re ... | 2010 | 20038687 |
| chemical synthesis and biological screening of 2-aminoimidazole-based bacterial and fungal antibiofilm agents. | a collection of 2-aminoimidazole/triazole amides has been synthesized and screened for antibiofilm activity. this class of small molecules was found to modulate the biofilm activity of pseudomonas aeruginosa, a multidrug-resistant strain of acinetobacter baumannii (mdrab), a methicillin-resistant staphylococcus aureus strain (mrsa), escherichia coli, rhodospirillum salexigens, staphylococcus epidermidis, vibrio vulnificus, and vancomycin-resistant enterococcus faecium as well as the yeast candid ... | 2010 | 20049758 |
| role of rpos in the susceptibility of low salinity-adapted vibrio vulnificus to environmental stresses. | vibrio vulnificus is an opportunistic pathogen commonly found in oyster and marine environments, which frequently encounters low salinity stress in its natural and food processing environment. in this study, the responses of a v. vulnificus wild-type strain c78140o and its rpos isogenic mutant ah1 to sublethal low salinity were examined to investigate the role of rpos in this response. both strains, adapted in low salinity (0.4% nacl), were protected against the lethal low salinity (0.1% nacl), ... | 2010 | 20051307 |
| expression, crystallization and preliminary x-ray diffraction analysis of a modification subunit of a putative type i restriction enzyme from vibrio vulnificus yj016. | modification (hsdm) and specificity (hsds) subunits are constituents of an active methyltransferase (mtase) of multifunctional type i restriction enzymes. to provide a molecular background on hsdm, a putative hsdm gene from vibrio vulnificus yj016 (hsdm_vv) was cloned and the expressed protein was purified and crystallized from 22%(w/v) polyethylene glycol 8000, 0.02 m imidazole ph 7.5 and 5 mm beta-mercaptoethanol. diffraction data were collected to 1.86 a resolution using synchrotron radiation ... | 2009 | 20054126 |
| role of tlr4 in the host response to vibrio vulnificus, an emerging pathogen. | the incidence of infection with vibrio vulnificus is increasing due to changing ecologic and demographic factors. most fatal cases are caused by septic shock that results from dysregulation of proinflammatory cytokines such as tumor necrosis factor-alpha (tnfalpha), presumably due to interaction of v. vulnificus components with toll-like receptors (tlrs). the goal of this study was to investigate the role of tlr4 in the host response to v. vulnificus. results obtained using v. vulnificus type st ... | 2010 | 20059544 |
| identification of a c-di-gmp-regulated polysaccharide locus governing stress resistance and biofilm and rugose colony formation in vibrio vulnificus. | as an etiological agent of bacterial sepsis and wound infections, vibrio vulnificus is unique among the vibrionaceae. its continued environmental persistence and transmission are bolstered by its ability to colonize shellfish, form biofilms on various marine biotic surfaces, and generate a morphologically and physiologically distinct rugose (r) variant that yields profuse biofilms. here, we identify a c-di-gmp-regulated locus (brp, for biofilm and rugose polysaccharide) and two transcription fac ... | 2010 | 20065022 |
| cefazolin plus minocycline against a clinical isolate of vibrio vulnificus: in vitro and animal studies. | cefotaxime plus minocycline has been shown to have synergistic activity against vibrio vulnificus; however, the clinical role of cefazolin in combination with minocycline in immunocompromised hosts has not been established. therefore, antimicrobial susceptibility of the v. vulnificus clinical isolate vv05191 was studied by the agar dilution method. antibacterial activity of cefazolin, minocycline, and a combination of the two drugs was investigated by time-kill studies in vitro and further exami ... | 2010 | 20093756 |
| the growth of vibrio vulnificus and the habitat of infected patients in kumamoto. | in japan, vibrio vulnificus (v. vulnificus) infection is very rare, and most infections have occurred in kumamoto prefecture (1), and especially around the ariake and yatsushiro seas. to investigate the relationship between the occurrence of v. vulnificus infection and environmental factors, including the salinity of seawater and the amount of rain in the ariake and yatsushiro seas, we measured the most probable number (mpn) of v. vulnificus in seawater and sea mud. in the ariake sea, we also ob ... | 2007 | 20103883 |
| detection of vibrio vulnificus in seafood, seawater and wastewater samples from a mediterranean coastal area. | vibrio vulnificus is an opportunistic human pathogen that may cause gastroenteritis, severe necrotizing soft-tissue infections and primary septicaemia, with a high lethality rate. illness is associated to ingestion of seafood or to the exposure of contaminated water. the aim of this work was to determine the occurrence of v. vulnificus in water and seafood samples from a coastal area near the mediterranean (valencia, spain). a taqman probe-based real-time pcr assay was optimised and applied to 2 ... | 2010 | 20106642 |
| direct interaction between quorum-sensing regulator smcr and rna polymerase is mediated by integration host factor to activate vvpe encoding elastase in vibrio vulnificus. | it has been suggested that quorum sensing is an important signal transduction system regulating the expression of numerous virulence genes in bacterial pathogens. we previously revealed that smcr, a luxr homologue of vibrio vulnificus, activates promoter s, an rpos-dependent promoter of vvpe encoding a potential virulence factor elastase and binds in vitro to a binding site centered at -196.5. in this study, chromatin immunoprecipitation assays and promoter deletion analyses demonstrated that sm ... | 2010 | 20110369 |
| in vitro time-kill activities of ciprofloxacin alone and in combination with the iron chelator deferasirox against vibrio vulnificus. | iron plays a major role in the growth and virulence of ferrophilic organisms like vibrio vulnificus. people who reside in the coastal areas with raw fish eating habits have a high risk of vibrio infection and aggressive therapy can only reduce their mortality. we investigated the in vitro efficacy of ciprofloxacin, a bactericidal drug used in v. vulnificus patients, and the orally active iron chelator deferasirox against v. vulnificus infection. we performed in vitro time-kill studies on two atc ... | 2010 | 20127132 |
| predicting the distribution of vibrio spp. in the chesapeake bay: a vibrio cholerae case study. | vibrio cholerae, the causative agent of cholera, is a naturally occurring inhabitant of the chesapeake bay and serves as a predictor for other clinically important vibrios, including vibrio parahaemolyticus and vibrio vulnificus. a system was constructed to predict the likelihood of the presence of v. cholerae in surface waters of the chesapeake bay, with the goal to provide forecasts of the occurrence of this and related pathogenic vibrio spp. prediction was achieved by driving an available mul ... | 2010 | 20145974 |
| predictors of mortality in skin and soft-tissue infections caused by vibrio vulnificus. | vibrio vulnificus infection can progress rapidly in skin or soft tissue, and it is potentially life-threatening. the purpose of the present study was to explore the predictors of mortality in patients with v. vulnificus infections of skin or soft tissue. | 2010 | 20151130 |
| using an improved tol2 transposon system to produce transgenic zebrafish with epinecidin-1 which enhanced resistance to bacterial infection. | in order to advance the application of antimicrobial peptides in aquaculture, transgenic zebrafish expressing the antimicrobial peptide, epinecidin-1, were developed and are reported on here. first, we cloned the zebrafish mylz2 promoter for this purpose. to characterize the activity of the mylz2 promoter, various fragments of it were analyzed using a firefly luciferase transient expression assay, in which maximum promoter activity was found with a 2.5-kb fragment. in addition, the 2.5-kb fragme ... | 2010 | 20153437 |
| a comparative epizootiologic study of the two fish-pathogenic serovars of vibrio vulnificus biotype 2. | vibrio vulnificus biotype 2 is subdivided into two main serovars, serovar e, able to infect fish and humans, and serovar a, only virulent for fish. serovar e emerged in 1976 as the causative agent of a haemorrhagic septicaemia (warm-water vibriosis) affecting eels cultured in brackish water. serovar a emerged in 2000 in freshwater-cultured eels vaccinated against serovar e, causing warm-water vibriosis with fish showing a haemorrhagic intestine as the main differential sign. the aim of the prese ... | 2010 | 20158583 |
| crystal structure of the transcriptional activator hlyu from vibrio vulnificus cmcp6. | hlyu is a transcription factor of the arsr/smtb family and activates the expression of the pathogenic vibrio vulnificus rtx toxin. in contrast to the other metal-responding arsr/smtb proteins, hlyu does not sense metal ions. to provide its structural information, we elucidated the crystal structure of hlyu from v. vulnificus cmcp6 (hlyu_vv). the monomeric hlyu_vv architecture of five alpha-helices and two beta-strands, some of which constitute a typical dna-binding winged helix-turn-helix (whth) ... | 2010 | 20178784 |
| crystal structure of smcr, a quorum-sensing master regulator of vibrio vulnificus, provides insight into its regulation of transcription. | quorum sensing has been implicated as an important global regulatory system controlling the expression of numerous virulence factors in bacterial pathogens. smcr, a homologue of vibrio harveyi luxr, has been proposed as a quorum-sensing master regulator of vibrio vulnificus, an opportunistic human pathogen. previous studies demonstrated that smcr is essential for the survival and pathogenesis of v. vulnificus, indicating that inhibiting smcr is an attractive approach to combat infections by the ... | 2010 | 20178981 |
| bacterial and viral pathogens in live oysters: 2007 united states market survey. | two samples of market oysters, primarily from retail establishments, were collected twice each month in each of nine states during 2007. samples were shipped refrigerated overnight to five u.s. food and drug administration laboratories on a rotating basis and analyzed by most probable number (mpn) for total and pathogenic vibrio parahaemolyticus and v. vulnificus numbers and for the presence of toxigenic v. cholerae, salmonella spp., norovirus (nov), and hepatitis a virus (hav). levels of indica ... | 2010 | 20190085 |
| involvement of capsular polysaccharide via a tlr2/nf-kappab pathway in vibrio vulnificus-induced il-8 secretion of human intestinal epithelial cells. | in a previous study, we reported that a wbpp gene mutation in vibrio vulnificus was significantly impaired in its ability to synthesize surface capsular polysaccharide (cps). in this study, we evaluated the functions of the v. vulnificus capsular polysaccharide on interleukin (il)-8 production, as well as its underlying mechanisms in human intestinal epithelial cells. the cps-defective wbpp mutant induced significantly lower levels of il-8 production, il-8 gene promoter activation and nf-kappab ... | 2010 | 20198307 |
| identification of a conserved membrane localization domain within numerous large bacterial protein toxins. | vibrio cholerae is the causative agent of the diarrheal disease cholera. many virulence factors contribute to intestinal colonization and disease including the multifunctional autoprocessing rtx toxin (martx(vc)). the rho-inactivation domain (rid) of martx(vc) is responsible for inactivating the rho-family of small gtpases, which leads to depolymerization of the actin cytoskeleton. based on a deletion analysis of rid to determine the minimal functional domain, we have identified a subdomain at t ... | 2010 | 20212166 |
| evidence that vibrio vulnificus ahpc2 is essential for survival under high salinity by modulating intracellular level of ros. | expression of ahpc2 encoding an alkyl hydroperoxide reductase of vibrio vulnificus, a foodborne pathogen, was incrementally induced depending on nacl concentrations in the culture. growth of the ahpc2 mutant was significantly impaired with longer lag phase and lower growth rate when cultured under high salinity. ros was accumulated in v. vulnificus cells when stressed by exposure to high salinity, and the ahpc2 mutant accumulated higher level of ros as compared with the parental wild type. conse ... | 2010 | 20221741 |
| clinical features of vibrio vulnificus infections in the coastal areas of the ariake sea, japan. | vibrio vulnificus infection can result in necrotizing fasciitis and sepsis and is associated with high mortality. most patients infected with this microbe have liver dysfunction as an underlying disease. however, because of the sporadic nature of outbreaks and unidentified cases, extensive evaluation of clinical features and identification of factors affecting prognosis have not been performed. we retrospectively analyzed 37 cases in japan from 1984 to 2008 to review clinical features and to ide ... | 2010 | 20229050 |
| laboratory indicators for early detection and surgical treatment of vibrio necrotizing fasciitis. | vibrio necrotizing fasciitis is a rare and life-threatening soft tissue infection, with fulminant clinical courses and high mortality rates. however, the lack of specific disease characteristics and diagnostic tools during the initial examination may delay diagnosis. | 2010 | 20232179 |
| functional characterization of the ilpa protein of vibrio vulnificus as an adhesin and its role in bacterial pathogenesis. | vibrio vulnificus is a gram-negative bacterium that causes a fatal septicemia. one of its virulence factors is a membrane-bound lipoprotein, ilpa, which can induce cytokine production in human immune cells. in the present study, the role of ilpa as an adhesion molecule was investigated. an ilpa-deleted v. vulnificus mutant showed significantly decreased adherence to int-407 human intestinal epithelial cells, which in turn resulted in reduced cytotoxicity. the deltailpa mutant recovered the adher ... | 2010 | 20308294 |
| assorted ribotypes of vibrio vulnificus human and environmental isolates. | we subtyped 117 vibrio vulnificus isolates from 85 v. vulnificus sepsis patients and 32 environmental samples by performing automated ribotyping for the purpose of molecular epidemiological study. although there was one indistinguishable ribotype among the four human isolates and one environmental isolate, taken as a whole, the ribotypes were highly diverse regardless of sample sources. we report here for the first time the assorted ribotypes of v. vulnificus human and environmental isolates in ... | 2010 | 20332574 |
| presence of typical and atypical virulence genes in vibrio isolates belonging to the harveyi clade. | the study was aimed at investigating the presence of typical and atypical virulence genes in isolates belonging to the harveyi clade (vibrio harveyi and vibrio campbellii). | 2010 | 20345385 |
| pathogenic vibrio activate nlrp3 inflammasome via cytotoxins and tlr/nucleotide-binding oligomerization domain-mediated nf-kappa b signaling. | vibrio vulnificus and vibrio cholerae are gram-negative pathogens that cause serious infectious disease in humans. the beta form of pro-il-1 is thought to be involved in inflammatory responses and disease development during infection with these pathogens, but the mechanism of beta form of pro-il-1 production remains poorly defined. in this study, we demonstrate that infection of mouse macrophages with two pathogenic vibrio triggers the activation of caspase-1 via the nlrp3 inflammasome. activati ... | 2010 | 20348425 |
| assimilation of metal ions bound to porphyrins or porphyrin-peptides by vibrio vulnificus, a human pathogen inhabiting estuarine and marine environments. | vibrio vulnificus, a ubiquitous microorganism in aquatic environments, causes serious septicemia to the immunocompromised host. in addition to protoheme, this species can utilize fe-tcpp [ferric tetrakis (4-carboxyphenyl) porphine] as an iron source. in the present study, heme c bound covalently to the protein in cytochrome c, as well as the fe-tcpp complex formed with a nanopeptide with a high affinity, was found to be useful iron sources for v. vulnificus. this bacterium was also revealed to u ... | 2010 | 20361516 |
| coincidental double erythema caused by vibrio vulnificus. | 2010 | 20398697 | |
| adaptation of vibrio vulnificus and an rpos mutant to bile salts. | vibrio vulnificus is an opportunistic pathogen commonly found in oyster and marine environments, which frequently encounters different stresses in its natural habitat, food processing environment and during infection. in this paper, the adaptation of v. vulnificus to bile and the role of rpos in this process were examined using a wild-type strain and an rpos isogenic mutant. adaptation to bile was readily induced in the exponential phase cells in phosphate-buffered saline with 2% bile salts, and ... | 2010 | 20406715 |
| prognostic factors for primary septicemia and wound infection caused by vibrio vulnificus. | the purpose of this study was to explore the predictive factors for mortality in primary septicemia or wound infections caused by vibrio vulnificus. | 2010 | 20466220 |
| tilapia hepcidin (th)2-3 as a transgene in transgenic fish enhances resistance to vibrio vulnificus infection and causes variations in immune-related genes after infection by different bacterial species. | hepcidin is an antimicrobial peptide (amp) secreted by the liver during inflammation that plays a central role in mammalian iron homeostasis. but the function of hepcidin in fish is still not completely understood. we recently described three different hepcidins (named tilapia hepcidin (th)1-5, th2-2, and th2-3) from tilapia oreochromis mossambicus, the cdna sequences were determined, the predicted peptides were synthesized, and the th2-3 peptide showed antimicrobial activity against several bac ... | 2010 | 20470893 |
| foodborne pathogens in retail oysters in south china. | to investigate the occurrence of important foodborne pathogens in shellstock pacific oysters in the food markets in south china. | 2010 | 20486433 |
| serum antibodies to vibrio vulnificus biotype 3 lipopolysaccharide and susceptibility to disease caused by the homologous v. vulnificus biotype. | in 1996 an outbreak of severe soft tissue infections caused by vibrio vulnificus unexpectedly erupted in fish consumers in israel with relatively little morbidity in fish farmers. to test the hypothesis that recurrent exposure of fishermen to the virulent strain may have provided protection against severe or symptomatic disease, we investigated the association between the immune response to v. vulnificus biotype 3 lipopolysaccharide (bt3 lps) and disease susceptibility in fish farmers and fish c ... | 2011 | 20492749 |
| genetic characterization of vibrio vulnificus strains from tilapia aquaculture in bangladesh. | outbreaks of vibrio vulnificus wound infections in israel were previously attributed to tilapia aquaculture. in this study, v. vulnificus was frequently isolated from coastal but not freshwater aquaculture in bangladesh. phylogenetic analyses showed that strains from bangladesh differed remarkably from isolates commonly recovered elsewhere from fish or oysters and were more closely related to strains of clinical origin. | 2010 | 20495047 |
| genistein inhibits vibrio vulnificus adhesion and cytotoxicity to hela cells. | vibrio vulnificus (v. vulnificus) is a useful model for bacterial septicemia as the bacterial infection generates a wide pathogenic spectrum in addition to a high mortality rate. this study was aimed at investigating the effects of genistein on the growth, cytotoxicity, adhesion, and the mouse mortality caused by v. vulnificus. the results of our study indicated that genistein (50 or 100 mg/l) effectively minimized the morphologic damages and inhibited acute death of hela cells by v. vulnificus. ... | 2010 | 20512479 |
| expression of divergent lsu rrna genes in the vibrio vulnificus cmcp6 genome during both infection and non-pathogenic stages. | the vibrio vulnificus cmcp6 genome harbors nine copies of divergent large subunit (lsu) rrna genes that may express and constitute four kinds of lsu rrna molecules in a single cell. primer extension analyses showed that these heterogeneous lsu rrna transcripts are all expressed and assembled into ribosomes during both infection and nonpathogenic stages. phylogenetic analyses of the internal transcribed spacer between ssu and lsu genes indicated that rrna operons of v. vulnificus cmcp6 can be clu ... | 2011 | 20526601 |
| a fatal case of vibrio vulnificus cellulitis with septicaemia. | vibrio vulnificus is a gram-negative marine bacterium that may cause local wound infection, distinctive soft tissue infection, gastroenteritis and septicaemia with a high mortality rate. a healthy man presented with severe abdominal pain, diarrhoea and fever followed by development of multiple blisters, cellulitis and necrotizing fasciitis of the lower limbs, who progressed rapidly to fulminant sepsis caused by this organism. vibrio vulnificus septicaemia should be suspected in the presence of s ... | 2009 | 20527281 |
| vibrio illness in florida, 1998-2007. | this study characterized the current epidemiology of vibrio infections in florida and examined cases reported from 1998 to 2007. logistic regression was used to determine risk of death. there were 834 vibrio infections in 825 individuals (average annual incidence rate 4·8/1,000,000). common vibrio species reported were vibrio vulnificus (33%), v. parahaemolyticus (29%), and v. alginolyticus (16%). most exposures were attributed to wounds (42%), and the most common clinical syndromes were wound i ... | 2011 | 20546636 |
| resveratrol modulates rtx toxin-induced cytotoxicity through interference in adhesion and toxin production. | host-parasite contact is a prerequisite for the acute cytotoxicity of vibrio vulnificus, which is mediated primarily by rtxa1, a repeat in toxin (rtx) toxin. we found that resveratrol (at 10 or 30 microm), a natural polyphenol, protected hela cells from v. vulnificus cytotoxicity. to further characterize the underlying mechanism, the effect of resveratrol was investigated at the level of the host-microbe interactions. we studied the effects of resveratrol on adhesion, motility, cytotoxicity, and ... | 2010 | 20553907 |
| quantitative detection of vibrio vulnificus in raw oysters by real-time loop-mediated isothermal amplification. | vibrio vulnificus is a significant cause of seafood-related morbidity and mortality in the united states. rapid and sensitive detection assays are needed to facilitate better control of potential v. vulnificus infections from seafood consumption. recently, loop-mediated isothermal amplification (lamp) has been applied to detect v. vulnificus. in this study, the lamp assay was coupled with either fluorescence- or turbidity-based real-time platforms for the quantitative detection of v. vulnificus ... | 2010 | 20584557 |
| multiplex pcr assays for simultaneous detection and characterization of vibrio vulnificus strains. | vibrio vulnificus is a major cause of seafood-related deaths in the united states. several biomarkers, e.g. the virulence-correlated gene (vcg), 16s rrna, and the capsular polysaccharide operon (cps) have been used to differentiate virulent- from nonvirulent-type v. vulnificus strains. in this study, we combined the use of these biomarkers with a species-specific v. vulnificus cytolysin/haemolysin gene (vvha) to develop two pairs of multiplex pcr assays that simultaneously detect and characteriz ... | 2010 | 20586937 |
| a description and illustration of a necrotizing fasciitis by john bell in 1801, hypothetically caused by vibrio vulnificus. | we present an overlooked and noteworthy historical case and illustration of a necrotizing fasciitis, observed and written by john bell (1763-1820), first published in 1801. considering the setting and the clinical presentation, we hypothesize that the pathogen responsible was the species vibrio vulnificus. the typical clinical course of a rapidly progressive illness preceded by saltwater exposure shortly followed by the development of a hemorrhagic skin lesion, with a fatal outcome, should stron ... | 2010 | 20594887 |
| immunomagnetic separation of vibrio vulnificus with antiflagellar monoclonal antibody. | raw oysters are primary vectors for vibrio vulnificus infections, and a rapid detection method for v. vulnificus in raw oysters before distribution would be an indispensable tool for the seafood industry. one approach to improving the recovery and detection of v. vulnificus without sacrificing assay time is through the use of immunomagnetic separation (ims). the aim of this study was to develop and optimize an ims protocol using anti-h (antiflagellar) antibody for determining the level of v. vul ... | 2010 | 20615341 |
| regulation of the vibrio vulnificus vvpe expression by cyclic amp-receptor protein and quorum-sensing regulator smcr. | in vibrio vulnificus, camp-receptor protein (crp) and the quorum-sensing regulator smcr are simultaneously and cooperatively required for the metalloprotease vvpe gene expression, rather than sequentially in a regulatory cascade. however, this study shows a new temporal and functional sequence between the two factors in regulating vvpe expression. a crp mutation inhibited vvpe expression with growth impairment from early stage. in contrast, a smcr mutation inhibited vvpe expression only at the l ... | 2010 | 20638468 |
| clinical outcomes and prognostic factors for patients with vibrio vulnificus infections requiring intensive care: a 10-yr retrospective study. | vibrio vulnificus infection is uncommon but potentially life-threatening. the aim of this study was to evaluate clinical outcomes and prognostic factors for patients with v. vulnificus infections admitted to an intensive care unit. | 2010 | 20657269 |
| an unusual case of sepsis with both vibrio vulnificus and enterococcus casseliflavus. | an 87-year-old man with metastatic prostate cancer on prior dexamethasone and chemotherapy presented to the emergency department with fever, hypotension and diarrhea. blood cultures at the time of admission revealed both vibrio vulnificus and enterococcus casseliflavus. raw oysters harvested from a louisiana marsh were consumed 12 and 13 days pre-admission. v. vulnificus sepsis typically manifests within one to three days of exposure, though prior reports have indicated infections as late as sev ... | 2010 | 20666168 |
| a highly sensitive and specific multiplex pcr assay for simultaneous detection of vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus. | to develop an effective multiplex pcr for simultaneous and rapid detection of vibrio cholerae, vibrio vulnificus and vibrio parahaemolyticus, the three most important vibrio species that can cause devastating health hazards among human. | 2010 | 20666989 |
| outer membrane vesicles of vibrio vulnificus deliver cytolysin-hemolysin vvha into epithelial cells to induce cytotoxicity. | the gram-negative bacterium vibrio vulnificus produces cytotoxins that induce the acute death of host cells. however, the secretory mechanisms of such cytotoxins have not been extensively studied. previously, we reported that substantial amounts of v. vulnificus cytolysin-hemolysin (vvha) are produced in vivo during the bacterial infection in mice and that this cytotoxin, in conjunction with rtxa1, mediates cytotoxicity. in this study, we investigated whether v. vulnificus cells release outer me ... | 2010 | 20682286 |