Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular characterization of a cryptic plasmid from campylobacter jejuni. | campylobacter jejuni is a leading bacterial cause of human enterocolitis. molecular genetic characterization of this pathogen has been hampered by the lack of genetic tools that are functional in this organism. cloning vectors commonly used in other organisms usually do not replicate within c. jejuni. to develop a system for functional analysis of c. jejuni genes, a small plasmid (pcj01) identified in a poultry isolate of c. jejuni was sequenced and characterized in this study. by using inverse ... | 2001 | 11322827 |
| come, a competence protein from neisseria gonorrhoeae with dna-binding activity. | neisseria gonorrhoeae is naturally able to take up exogenous dna and undergo genetic transformation. this ability correlates with the presence of functional type iv pili, and uptake of dna is dependent on the presence of a specific 10-bp sequence. among the known competence factors in n. gonorrhoeae, none has been shown to interact with the incoming dna. here we describe come, a dna-binding protein involved in neisserial competence. the gene come was identified through similarity searches in the ... | 2001 | 11325945 |
| rapid pulsed-field gel electrophoresis protocol for subtyping of campylobacter jejuni. | we developed a rapid pulsed-field gel electrophoresis (pfge) protocol for subtyping campylobacter isolates based on the standardized protocols used by pulsenet laboratories for the subtyping of other food-borne bacterial pathogens. various combinations of buffers, reagents, reaction conditions (e.g., cell suspension concentration, lysis time, lysis temperature, and restriction enzyme concentration), and electrophoretic parameters were evaluated in an effort to devise a protocol that is simple, r ... | 2001 | 11326009 |
| comparison of two methods for serotyping campylobacter spp. | two serotyping schemes (penner and laboratory of enteric pathogens [lep]) based on soluble heat-stable antigens were used to analyze 3,788 campylobacter sp. isolates. a significant percentage (36.6%) was untypeable using lep serotyping; greater cross-reaction was also observed. the relative discrimination capabilities of the techniques were similar. penner serotyping fulfils more of the requisite criteria for typing methods. | 2001 | 11326013 |
| axonal guillain-barré syndrome: a critical review. | axonal guillain-barré syndrome (gbs) was first described by feasby et al. in 1986, challenging the existent notion of gbs being a primarily demyelinating disease. the severe course and slow recovery commonly seen in these patients was ascribed to axonal degeneration. other authors challenged this claim on several grounds. amidst these controversies, epidemics of a similar illness were reported from china, which were given the acronym aman, having exclusive motor involvement in contrast to the ca ... | 2001 | 11328201 |
| antecedent symptoms in guillain-barré syndrome: an important indicator for clinical and serological subgroups. | to examine whether guillain-barré syndrome (gbs) can be classified in clinical and immunological subgroups based on the type of prior illness. background - the existence of antecedent symptoms supports the diagnosis of gbs in patients who experience acute muscle weakness progression. however, little is known about additional meanings of determining antecedent symptoms. | 2001 | 11328202 |
| the accuracy of serologic diagnosis of helicobacter pylori infection in school-aged children of mixed ethnicity. | the present study evaluated two non-invasive diagnostic methods for h. pylori infection in children, i.e. an in-house elisa using sonicated campylobacter jejuni antigen for absorption of cross-reacting antibodies and an immunoblot kit (helico blot 2.0, genelabs, singapore). 13c -urea breath test (13c-ubt) was used as reference method: sera and questionnaires were collected from 695/858 (81%) swedish school children with mixed ethnic backgrounds within a cross-sectional, community-based study. of ... | 2001 | 11328362 |
| fecal shedding and antimicrobial susceptibility of selected bacterial pathogens and a survey of intestinal parasites in free-living waterfowl. | free-living waterfowl residing in metropolitan parks in central ohio were surveyed for the fecal shedding and antimicrobial susceptibility patterns of campylobacter jejuni, escherichia coli, salmonella spp., and pasteurella multocida. in addition, a survey for intestinal parasites was also conducted in these same waterfowl to determine parasite burdens in free-living waterfowl. prevalences of 67%, 50%, and 0.2% of e. coli, c. jejuni, and salmonella spp., respectively, were observed for all water ... | 2001 | 11332473 |
| [predominance of campylobacter jejuni in cases of bacterial gastroenteritis in the adult]. | 2001 | 11333596 | |
| [bacteremia due to campylobacter jejuni: report of two cases]. | 2001 | 11333614 | |
| molecular mimicry in campylobacter jejuni and helicobacter pylori lipopolysaccharides: contribution of gastrointestinal infections to autoimmunity. | molecular mimicry of host structures by the saccharide portion of lipopolysaccharides (lps) of the gastrointestinal pathogens campylobacter jejuni and helicobacter pylori is thought to be associated with the development of autoimmune sequelae. c. jejuni, a leading cause of gastroenteritis, is the most common antecedent infection in guillain-barré syndrome (gbs), an inflammatory neuropathy. chemical analyses of the core oligosaccharides of neuropathy-associated c. jejuni strains have revealed str ... | 2001 | 11334489 |
| secretion of the virulence-associated campylobacter invasion antigens from campylobacter jejuni requires a stimulatory signal. | campylobacter jejuni are a common cause of human diarrheal illness. previous work has demonstrated that c. jejuni synthesize a novel set of proteins upon coculturing with epithelial cells, some of which are secreted. the secreted proteins have been collectively referred to as campylobacter invasion antigens (cia proteins). metabolic labeling experiments revealed that cia protein synthesis and secretion are separable and that secretion is the rate-limiting step of these processes. additional work ... | 2001 | 11343209 |
| polarity in action: asymmetric protein localization in bacteria. | 2001 | 11344132 | |
| functional analysis of rela and rsha, two rela/spot homologues of streptomyces coelicolor a3(2). | deletion of the (p)ppgpp synthetase gene, rela, of streptomyces coelicolor a3(2) results in loss of production of the antibiotics actinorhodin (act) and undecylprodigiosin (red) and delayed morphological differentiation when the mutant is grown under conditions of nitrogen limitation. to analyze the role of (p)ppgpp as an intracellular signaling molecule for the initiation of antibiotic production, several c-terminally deleted derivatives of s. coelicolor rela that could potentially function in ... | 2001 | 11344157 |
| microbiological status of commercially available medicinal herbal drugs--a screening study. | one hundred and thirty-eight medicinal herbal drugs obtained from different suppliers were examined for microbial contaminants and for the detectability of pathogenic microorganisms. for this purpose, several microbiological standard parameters (total aerobic mesophilic count, enterobacteria, coliforms, aerobic sporeformers, yeasts and moulds, enterococci, lactobacilli, pseudomonades and aeromades) and selective methods for the detection of indicator microorganisms and pathogens (e. coli, entero ... | 2001 | 11345700 |
| enrichment media for isolation of campylobacter jejuni from inoculated ground beef and chicken skin under normal atmosphere. | the efficiency of hunt broth containing oxyrase was compared with the gas replacement method for detection of campylobacter jejuni in inoculated ground beef and chicken skin. five strains of c. jejuni were inoculated individually into samples and cultured with various media under conditions generated by either flushing with a mixture of gases or supplementing with oxyrase. oxyrase media added with 7% lysed blood, 2.5% charcoal, or 6% ground cooked meat were compared with examinations from chicke ... | 2001 | 11347991 |
| effects of feed withdrawal and transport on cecal environment and campylobacter concentrations in a swine surgical model. | the objective of the present study was to evaluate how feed withdrawal and transportation influenced the cecal environment and cecal populations of campylobacter in swine. four miniature yucatan gilts (8.8 kg), naturally infected with campylobacter jejuni, were surgically implanted with cecal cannulas. the gilts were fasted for 48 h. samples of cecal contents were collected for 7 days prior to and for 7 days after the fast, and mean values were determined for ph, volatile fatty acids (vfa), and ... | 2001 | 11348010 |
| mycobacterium bovis bcg reca deletion mutant shows increased susceptibility to dna-damaging agents but wild-type survival in a mouse infection model. | pathogenic microorganisms possess antioxidant defense mechanisms for protection from reactive oxygen metabolites which are generated during the respiratory burst of phagocytic cells. these defense mechanisms include enzymes such as catalase, which detoxifies reactive oxygen species, and dna repair systems, which repair damage resulting from oxidative stress. to (i) determine the relative importance of the dna repair system when oxidative stress is encountered by the mycobacterium tuberculosis co ... | 2001 | 11349014 |
| polymorphisms in pilin glycosylation locus of neisseria meningitidis expressing class ii pili. | we have located a locus, pgl, in neisseria meningitidis strain nmb required for the glycosylation of class ii pili. between five and eight open reading frames (orfs) (pglf, pglb, pglc, pglb2, orf2, orf3, orf8, and avta) were present in the pgl clusters of different meningococcal isolates. the class i pilus-expressing strains neisseria gonorrhoeae ms11 and n. meningitidis mc58 each contain a pgl cluster in which orf2 and orf3 have been deleted. strain nmb and other meningococcal isolates which ex ... | 2001 | 11349019 |
| perr controls oxidative stress resistance and iron storage proteins and is required for virulence in staphylococcus aureus. | the staphylococcus aureus genome encodes three ferric uptake regulator (fur) homologues: fur, perr, and zur. to determine the exact role of perr, we inactivated the gene by allelic replacement using a kanamycin cassette, creating strain mjh001 (perr). perr was found to control transcription of the genes encoding the oxidative stress resistance proteins catalase (kata), alkyl hydroperoxide reductase (ahpcf), bacterioferritin comigratory protein (bcp), and thioredoxin reductase (trxb). furthermore ... | 2001 | 11349039 |
| superoxide dismutase-deficient mutants of helicobacter pylori are hypersensitive to oxidative stress and defective in host colonization. | superoxide dismutase (sod) is a nearly ubiquitous enzyme among organisms that are exposed to oxic environments. the single sod of helicobacter pylori, encoded by the sodb gene, has been suspected to be a virulence factor for this pathogenic microaerophile, but mutations in this gene have not been reported previously. we have isolated mutants with interruptions in the sodb gene and have characterized them with respect to their response to oxidative stress and ability to colonize the mouse stomach ... | 2001 | 11349073 |
| comparison of the activity spectra against pathogens of bacterial strains producing a mutacin or a lantibiotic. | the increase of drug resistance among bacterial pathogens is currently a major threat in hospital settings. new and more efficient antibiotic compounds have to be developed to fight infectious diseases. in the present work, a deferred antagonism test was used to determine the activity of different bacterial strains producing either a mutacin or a lantibiotic against bacterial pathogens. the mutacins a, b, c, d, i, k, l, m, and nisins a and z were active against all enterococci tested. mutacins a ... | 2001 | 11358172 |
| a phase-variable capsule is involved in virulence of campylobacter jejuni 81-176. | campylobacter jejuni strain 81-176 (hs36, 23) synthesizes two distinct glycan structures, as visualized by immunoblotting of proteinase k-digested whole-cell preparations. a site-specific insertional mutant in the kpsm gene results in loss of expression of a high-molecular-weight (hmw) glycan (apparent mr 26 kda to > 85 kda) and increased resolution of a second ladder-like glycan (apparent mr 26-50 kda). the kpsm mutant of 81-176 is no longer typeable in either hs23 or hs36 antisera, indicating ... | 2001 | 11359581 |
| hiv and peripheral neuropathy. | manifestations and treatments of many types of common illnesses associated with peripheral neuropathy (pn) in hiv-infected people are presented. specific topics examine guillain-barre syndrome, chronic inflammatory demyelinating neuropathy, mononeuritis multiplex, distal symmetrical polyneuropathy, cmv polyradiculopathy, neurotoxic neuropathy, and campylobacter jejuni infection. physicians who treat hiv-1 patients should be aware that pns are common, disabling, and treatable complications of hiv ... | 1995 | 11362726 |
| genetic characterization of the klebsiella pneumoniae waa gene cluster, involved in core lipopolysaccharide biosynthesis. | a recombinant cosmid containing genes involved in klebsiella pneumoniae c3 core lipopolysaccharide biosynthesis was identified by its ability to confer bacteriocin 28b resistance to escherichia coli k-12. the recombinant cosmid contains 12 genes, the whole waa gene cluster, flanked by kbl and coad genes, as was found in e. coli k-12. pcr amplification analysis showed that this cluster is conserved in representative k. pneumoniae strains. partial nucleotide sequence determination showed that the ... | 2001 | 11371519 |
| changes in the carriage of campylobacter strains by poultry carcasses during processing in abattoirs. | the recent development of simple, rapid genotyping techniques for campylobacter species has enabled investigation of the determinative epidemiology of these organisms in a variety of situations. in this study we have used the technique of fla typing (pcr-restriction fragment length polymorphism analysis of the flaa and flab genes) to identify the sources of strains contaminating the carcasses of five campylobacter-positive and two campylobacter-negative broiler flocks during abattoir processing. ... | 2001 | 11375174 |
| evidence that certain clones of campylobacter jejuni persist during successive broiler flock rotations. | through the national surveillance program for campylobacter spp., nine broiler chicken farms that were infected with campylobacter jejuni in at least five rotations in 1998 were identified. one additional farm, located at the island of bornholm where divided slaughter is used extensively, was also selected. twelve broiler houses located on 10 farms were included in the study. the c. jejuni isolates collected from the selected houses during the surveillance were typed using fla typing and macrore ... | 2001 | 11375189 |
| etiology of children's diarrhea in montevideo, uruguay: associated pathogens and unusual isolates. | we studied microorganisms associated with infant diarrhea in a group of 256 children admitted to a public pediatric hospital in montevideo, uruguay. diagnostic procedures were updated to optimize detection of potential pathogens, which were found in 63.8% of cases, and to be able to define their characteristics down to molecular or antigenic type. coinfection with two or more agents was detected in more than one-third of positive studies. escherichia coli enteric virotypes, especially enteropath ... | 2001 | 11376047 |
| coinfection of enteric helicobacter spp. and campylobacter spp. in cats. | during a 6-year period, 64 of 227 commercially reared cats had microaerobic bacteria isolated from their feces. all the isolates were initially identified as campylobacter-like organisms based on biochemical and phenotypic characteristics. dna extractions from 51 of these isolates were subjected to pcr using primers specific for helicobacter spp. and campylobacter spp. of the isolates, 92% (47 of 51 isolates) were positive for campylobacter spp., 41% (21 of 51 isolates) were positive for helicob ... | 2001 | 11376052 |
| rapid identification of campylobacter spp. by melting peak analysis of biprobes in real-time pcr. | we describe rapid pcr-biprobe identification of campylobacter spp. this is based on real-time pcr with product analysis in the same system. the assay identifies enteropathogenic campylobacters to the species level on the basis of their degree of hybridization to three 16s ribosomal dna (rdna) biprobes. first-round symmetric pcr is performed with genus-specific primers which selectively target and amplify a portion of the 16s rrna gene common to all campylobacter species. second-round asymmetric ... | 2001 | 11376061 |
| comparative study using amplified fragment length polymorphism fingerprinting, pcr genotyping, and phenotyping to differentiate campylobacter fetus strains isolated from animals. | a collection of campylobacter fetus strains, including both c. fetus subsp. fetus and c. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by pcr and amplified fragment length polymorphism (aflp) analysis. phenotypic subspecies determination methods were unreliable. genotyping of the strains by pcr and aflp showed a clear discrimination between the two subspecies. | 2001 | 11376071 |
| identification of a putative chromosomal replication origin from helicobacter pylori and its interaction with the initiator protein dnaa. | the key elements of the initiation of helicobacter pylori chromosome replication, dnaa protein and putative oric region, have been characterized. the gene arrangement in the h.pylori dnaa region differs from that found in many other eubacterial dnaa regions (rnpa-rmph-dnaa-dnan-recf-gyrb). helicobacter pylori dnaa is flanked by two open reading frames with unknown function, while dnan-gyrb and rnpa-rmph loci are separated from the dnaa gene by 600 and 90 kb, respectively. we show that the dnaa g ... | 2001 | 11376143 |
| cloning and sequencing of the asta gene encoding arylsulfate sulfotransferase from salmonella typhimurium. | arylsulfate sulfotransferase (asst) transfers a sulfate group from a phenolic sulfate ester to a phenolic acceptor substrate. in the present study, the gene encoding asst was cloned from a genomic library of salmonella typhimurium. the gene was subcloned into the vector pkf3 and was sequenced. a recombinant clone harboring the gene was directly identified using a fluorescent assay. sequencing revealed two contiguous open reading frames (orfs) on the same strand. based on amino acid sequence homo ... | 2001 | 11379783 |
| tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. | tetracyclines were discovered in the 1940s and exhibited activity against a wide range of microorganisms including gram-positive and gram-negative bacteria, chlamydiae, mycoplasmas, rickettsiae, and protozoan parasites. they are inexpensive antibiotics, which have been used extensively in the prophlylaxis and therapy of human and animal infections and also at subtherapeutic levels in animal feed as growth promoters. the first tetracycline-resistant bacterium, shigella dysenteriae, was isolated i ... | 2001 | 11381101 |
| escherichia coli infections and hemolytic-uremic syndrome. | 2001 | 11387907 | |
| changes in campylobacter jejuni and campylobacter coli carriage rates in the zenica region [correction of zenica canton] of bosnia and herzegovina in the pre- and postwar periods. | 2001 | 11388169 | |
| the iron- and temperature-regulated cjrbc genes of shigella and enteroinvasive escherichia coli strains code for colicin js uptake. | a cosmid library of dna from colicin js-sensitive enteroinvasive escherichia coli (eiec) strain o164 was made in colicin js-resistant strain e. coli vcs257, and colicin js-sensitive clones were identified. sensitivity to colicin js was associated with the carriage of a three-gene operon upstream of and partially overlapping senb. the open reading frames were designated cjrabc (for colicin js receptor), coding for proteins of 291, 258, and 753 amino acids, respectively. tn7 insertions in any of t ... | 2001 | 11395459 |
| microbial origin of plant-type 2-keto-3-deoxy-d-arabino-heptulosonate 7-phosphate synthases, exemplified by the chorismate- and tryptophan-regulated enzyme from xanthomonas campestris. | enzymes performing the initial reaction of aromatic amino acid biosynthesis, 2-keto-3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthases, exist as two distinct homology classes. the three classic escherichia coli paralogs are aroa(i) proteins, but many members of the bacteria possess the aroa(ii) class of enzyme, sometimes in combination with aroa(i) proteins. aroa(ii) dahp synthases until now have been shown to be specifically dedicated to secondary metabolism (e.g., formation of ansamy ... | 2001 | 11395471 |
| use of continuous culture bioreactors for the study of pathogens such as campylobacter jejuni and escherichia coli o157 in biofilms. | 2001 | 11398452 | |
| potential intervention of campylobacter jejuni in the modulation of murine immune response. | campylobacter jejuni has been reported to produce different toxins that may modulate the immune response in both animals and humans. the effect of c. jejuni enterotoxin on the immune response was investigated in two groups of balb/c mice. one of them was inoculated intraperitoneally with 1010 colony forming units (cfu) of an enterotoxigenic strain (ccug 7580), and the second one with a non-enterotoxigenic strain (ccug 7440). the number of polymorphonuclear (pmn) cells from spleen increased in bo ... | 2001 | 11400072 |
| absence of clonality of campylobacter jejuni in serotypes other than hs:19 associated with guillain-barré syndrome and gastroenteritis. | guillain-barré syndrome (gbs) is recognized as a complication that occurs after campylobacter infection. certain penner serotypes, such as hs:19, are linked particularly to gbs in some parts of the world, and there is good evidence for restricted genetic diversity in these isolates. however, gbs also occurs after campylobacter infection due to other serotypes. therefore, we asked whether campylobacter jejuni non-hs:19 serotypes associated with gbs have a clonal structure and differ from strains ... | 2001 | 11400076 |
| molecular population genetic analysis of campylobacter jejuni hs:19 associated with guillain-barré syndrome and gastroenteritis. | infection with campylobacter jejuni serotype hs:19 is associated with the development of guillain-barré syndrome (gbs). to determine whether a particular hs:19 clone is associated with gbs, multilocus enzyme electrophoresis (mlee) was used to analyze a worldwide collection of isolates. there were 34 electropherotypes (ets) in 3 phylogenetic clusters among 83 c. jejuni isolates. cluster i contained all hs:19 strains, and a single et (et4) accounted for most hs:19 strains. hs:19 strains did not oc ... | 2001 | 11400077 |
| motility and the polar flagellum are required for aeromonas caviae adherence to hep-2 cells. | aeromonas caviae is increasingly being recognized as a cause of gastroenteritis, especially among the young. the adherence of aeromonads to human epithelial cells in vitro has been correlated with enteropathogenicity, but the mechanism is far from well understood. initial investigations demonstrated that adherence of a. caviae to hep-2 cells was significantly reduced by either pretreating bacterial cells with an antipolar flagellin antibody or by pretreating hep-2 cells with partially purified f ... | 2001 | 11401962 |
| cdta, cdtb, and cdtc form a tripartite complex that is required for cytolethal distending toxin activity. | campylobacter jejuni encodes a cytolethal distending toxin (cdt) that causes cells to arrest in the g(2)/m transition phase of the cell cycle. highly related toxins are also produced by other important bacterial pathogens. cdt activity requires the function of three genes: cdta, cdtb, and cdtc. recent studies have established that cdtb is the active subunit of cdt, exerting its effect as a nuclease that damages the dna and triggers cell cycle arrest. microinjection of cdtb into target cells led ... | 2001 | 11401974 |
| gastroenteritis-associated guillain-barré syndrome on the caribbean island curaçao. | the number of patients with guillain-barré syndrome (gbs) who have been observed in curaçao, the netherlands antilles, may be increasing. | 2001 | 11402102 |
| survival and death of salmonella typhimurium and campylobacter jejuni in processing water and on chicken skin during poultry scalding and chilling. | salmonella typhimurium and campylobacter jejuni were inoculated in scalding water, in chilled water, and on chicken skins to examine the effects of scalding temperature (50, 55, and 60 degrees c) and the chlorine level in chilled water (0, 10, 30, and 50 ppm), associated with the ages of scalding water (0 and 10 h) and chilled water (0 and 8 h), on bacterial survival or death. after scalding at 50 and 60 degrees c, the reductions of c. jejuni were 1.5 and 6.2 log cfu/ml in water and <1 and >2 lo ... | 2001 | 11403124 |
| antimicrobial susceptibility of intestinal bacteria from swiss poultry flocks before the ban of antimicrobial growth promoters. | from the crop and the caecum of swiss broilers slaughtered between november 1997 and january 1998, escherichia coli, enterococci, staphylococci, lactobacilli and campylobacter species were isolated. after identification to the genus or species level, their minimal inhibitory concentrations (mic's) for several clinically used antimicrobial agents were determined with the e-test stripes and compared to those from studies in other european countries. all strains of enterococcus faecalis (n = 38), e ... | 2001 | 11403390 |
| investigation of infection with campylobacter jejuni in a man with hypogammaglobulinaemia using pcr-single-stranded conformational polymorphism (pcr-sscp) typing. | this study investigated several episodes of infection of campylobacter jejuni in an immunocompromised male with hypogammaglobulinaemia, presenting with diarrhoea and bacteraemia over a 16-month period, by employing three phenotyping and four genotyping schemes, including the single-stranded conformational polymorphism (sscp) technique to establish if infection was reinfection or persistent infection. four isolates from blood culture and two faecal isolates of campylobacter jejuni were obtained f ... | 2001 | 11403407 |
| dna gyrase-mediated natural resistance to fluoroquinolones in ehrlichia spp. | fluoroquinolone susceptibility heterogeneity between various ehrlichia species has been previously demonstrated. in gram-negative bacteria, resistance to fluoroquinolones most often corresponds to specific amino acid variations in a portion of the protein sequence of the a subunit of dna gyrase (gyra), referred to as the quinolone resistance-determining region (qrdr). we suspected a similar mechanism to be responsible for natural resistance in some ehrlichia species. to verify this hypothesis, w ... | 2001 | 11408229 |
| genemarks: a self-training method for prediction of gene starts in microbial genomes. implications for finding sequence motifs in regulatory regions. | improving the accuracy of prediction of gene starts is one of a few remaining open problems in computer prediction of prokaryotic genes. its difficulty is caused by the absence of relatively strong sequence patterns identifying true translation initiation sites. in the current paper we show that the accuracy of gene start prediction can be improved by combining models of protein-coding and non-coding regions and models of regulatory sites near gene start within an iterative hidden markov model b ... | 2001 | 11410670 |
| range of cross reactivity of anti-gm1 igg antibody in guillain-barré syndrome. | the cross reactivity of anti-gm1 igg antibody with various gangliosides and asialo-gm1 in serum samples from 27 patients with guillain-barré syndrome was investigated. an enzyme linked immunosorbent assay (elisa) absorption study showed that anti-gm1 igg antibody cross reacted with asialo-gm1 in 52% of the patients, gm1b in 41%, gd1b in 22%, and galnac-gd1a in 19%, and that it did not cross react with gm2, gt1b, or gq1b. the antibody that cross reacted with gd1b was associated with a high freque ... | 2001 | 11413278 |
| sodium ion-driven serine/threonine transport in porphyromonas gingivalis. | porphyromonas gingivalis is an asaccharolytic, gram-negative bacterium that relies on the fermentation of amino acids for metabolic energy. when grown in continuous culture in complex medium containing 4 mm (each) free serine, threonine, and arginine, p. gingivalis assimilated mainly glutamate/glutamine, serine, threonine, aspartate/asparagine, and leucine in free and/or peptide form. serine and threonine were assimilated in approximately equal amounts in free and peptide form. we characterized ... | 2001 | 11418553 |
| characterization of an aminoacylase from the hyperthermophilic archaeon pyrococcus furiosus. | aminoacylase was identified in cell extracts of the hyperthermophilic archaeon pyrococcus furiosus by its ability to hydrolyze n-acetyl-l-methionine and was purified by multistep chromatography. the enzyme is a homotetramer (42.06 kda per subunit) and, as purified, contains 1.0 +/- 0.48 g-atoms of zinc per subunit. treatment of the purified enzyme with edta resulted in complete loss of activity. this was restored to 86% of the original value (200 u/mg) by treatment with zncl(2) (and to 74% by th ... | 2001 | 11418567 |
| the physiology and metabolism of campylobacter jejuni and helicobacter pylori. | 2001 | 11422557 | |
| post genome analysis of campylobacter jejuni. | 2001 | 11422559 | |
| animal models of campylobacter jejuni colonization and disease and the lessons to be learned from similar helicobacter pylori models. | 2001 | 11422561 | |
| guillain--barré syndrome and campylobacter jejuni infection. | 2001 | 11422570 | |
| evolution of gene order conservation in prokaryotes. | as more complete genomes are sequenced, conservation of gene order between different organisms is emerging as an informative property of the genomes. conservation of gene order has been used for predicting function and functional interactions of proteins, as well as for studying the evolutionary relationships between genomes. the reasons for the maintenance of gene order are still not well understood, as the organization of the prokaryote genome into operons and lateral gene transfer cannot poss ... | 2001 | 11423009 |
| serotype and genotype diversity and hatchery transmission of campylobacter jejuni in commercial poultry flocks. | we investigated the genotype and serotype diversity of campylobacter coli and c. jejuni in two parent flocks of adult hens and their offspring over two rotations in order to evaluate the role of hatchery mediated transmission and/or vertical transmission of campylobacters in broiler flocks. in total, 314 c. jejuni and 32 c. coli isolates from parent and broiler flocks and from the surroundings of broiler houses were typed by flagellin gene pcr/rflp (fla-typing), and selected isolates were also t ... | 2001 | 11423205 |
| comparison of genotypes and serotypes of campylobacter jejuni isolated from danish wild mammals and birds and from broiler flocks and humans. | the incidence of human infection with campylobacter jejuni is increasing in most developed countries and the reason for this is largely unknown. although poultry meat is considered to be a major source, it is evident that other reservoirs exist, possibly common to humans and poultry. environmental sources are believed to be important reservoirs of campylobacter infection in broiler chicken flocks. we investigated the potential importance of wildlife as a source of infection in commercial poultry ... | 2001 | 11425730 |
| natural transformation in mesophilic and thermophilic bacteria: identification and characterization of novel, closely related competence genes in acinetobacter sp. strain bd413 and thermus thermophilus hb27. | the mesophile acinetobacter sp. strain bd413 and the extreme thermophile thermus thermophilus hb27 display high frequencies of natural transformation. in this study we identified and characterized a novel competence gene in acinetobacter sp. strain bd413, coma, whose product displays significant similarities to the competence proteins coma and comec in neisseria and bacillus species. transcription of coma correlated with growth phase-dependent transcriptional regulation of the recently identifie ... | 2001 | 11425734 |
| production of bartonella genus-specific monoclonal antibodies. | monoclonal antibodies (mabs) which react with heat-resistant proteins with molecular masses of 32 to 33 kda of 14 different bartonella species were produced. these antibodies did not react with antigens of 26 diverse bacterial strains by microimmunofluorescence assay except mab b3d4, which reacted with chlamydia psittaci and chlamydia trachomatis at low titers. the identification of a common bartonella antigenic protein will make it possible to later produce a diagnostic antigen by cloning and e ... | 2001 | 11427441 |
| evaluation of methods for subtyping campylobacter jejuni during an outbreak involving a food handler. | in october 1998, the centers for disease control and prevention (cdc) assisted in an investigation of an outbreak of campylobacteriosis at a school in salina, kansas. twenty-two isolates were submitted from the kansas state public health laboratory to cdc, 9 associated with the outbreak and 13 epidemiologically unrelated sporadic isolates. pulsed-field gel electrophoresis (pfge) using smai and sali was initially used to validate the epidemiologic data. we then tested the ability of other subtypi ... | 2001 | 11427543 |
| helicobacter winghamensis sp. nov., a novel helicobacter sp. isolated from patients with gastroenteritis. | from 1997 to 1999 seven isolates of campylobacter-like organisms from five patients that were exhibiting symptoms of gastroenteritis, including fever, stomach malaise, and diarrhea, were investigated. the organisms were isolated from stool samples and found to exhibit a diverse colony morphology; hence multiple isolates were submitted from one of the patients. all isolates were found to be identical. the organisms were catalase, urease, alkaline phosphatase, and nitrate negative but oxidase and ... | 2001 | 11427547 |
| rpob sequence analysis of cultured tropheryma whippelii. | until recently no isolate of tropheryma whippelii was available, and therefore genetic studies were limited to those based on pcr amplification of conserved genes. in this study we determined the nucleotide sequence of rpob (encoding the beta-subunit of rna polymerase) from a cultured strain of t. whippelii using degenerate consensus pcr and genome walking. the t. whippelii rpob consists of 3,657 bp with a 50.4% gc content and encodes 1,218 amino acids with a calculated molecular mass of 138 kda ... | 2001 | 11427549 |
| genomic heterogeneity and o-antigenic diversity of campylobacter upsaliensis and campylobacter helveticus strains isolated from dogs and cats in germany. | a serotyping scheme based on heat-stable surface antigens was established for 101 campylobacter upsaliensis and 10 campylobacter helveticus strains isolated from 261 dogs and 46 cats of different ages originating from two geographically distinct regions in germany. the prevalence of c. upsaliensis varied between 27.8% in juvenile dogs (<12 months of age) and 55.4% in adult dogs (p < 0.05). of the cats, 19.6% harbored c. upsaliensis, whereas 21.7% carried c. helveticus. of the c. upsaliensis isol ... | 2001 | 11427567 |
| fluorogenic pcr-based quantitative detection of a murine pathogen, helicobacter hepaticus. | helicobacter hepaticus infection in mice is being used as an animal model for elucidating the pathogenesis of gastrointestinal and biliary diseases in humans. h. hepaticus, which forms a spreading film on selective agar, is not amenable to routine quantitative counts of organisms in tissues using a cfu method. in this study, a fluorogenic pcr-based assay was developed to quantitatively detect h. hepaticus in mouse ceca and feces using the abi prism 7700 sequence detection system. a pair of prime ... | 2001 | 11427576 |
| direct detection of legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of lightcycler pcr, in situ hybridization, direct fluorescence antigen detection, and culture. | we developed a rapid thermocycling, real-time detection (also known as real-time pcr) method for the detection of legionella species directly from clinical specimens. this method uses the lightcycler (roche molecular biochemicals, indianapolis, ind.) and requires approximately 1 to 2 h to perform. both a legionella genus pcr assay and legionella pneumophila species-specific pcr assay were designed. a total of 43 archived specimens from 35 patients were evaluated, including 19 bronchoalveolar lav ... | 2001 | 11427579 |
| basis of the superiority of cefoperazone amphotericin teicoplanin for isolating campylobacter upsaliensis from stools. | the optimum method for isolating campylobacter upsaliensis from stools has not been clearly defined. in a preliminary study, cefoperazone amphotericin teicoplanin (cat) selective medium isolated six c. upsaliensis strains which were not detected using modified cefoperazone charcoal deoxycholate (mccda). in order to identify the factors that underlie the superiority of cat over mccda for isolating c. upsaliensis, we examined the effect of incubation time and antibiotic content of culture media on ... | 2001 | 11427603 |
| a campylobacter jejuni gene associated with immune-mediated neuropathy. | 2001 | 11433317 | |
| [anti-gq1b antibodies: usefulness of its detection for the diagnosis of miller-fisher syndrome]. | to study the presence of anti-gq1b antibodies as a tool for the diagnosis of miller-fisher syndrome (mfs). | 2001 | 11440679 |
| negative selection of t cells by helicobacter pylori as a model for bacterial strain selection by immune evasion. | the majority of humans infected with helicobacter pylori maintain a lifelong infection with strains bearing the cag pathogenicity island (pai). h. pylori inhibits t cell responses and evades immunity so the mechanism by which infection impairs responsiveness was investigated. h. pylori caused apoptotic t cell death, whereas campylobacter jejuni did not. the induction of apoptosis by h. pylori was blocked by an anti-fas ab (zb4) or a caspase 8 inhibitor. in addition, a t cell line with the fas re ... | 2001 | 11441100 |
| screening of patients with complex regional pain syndrome for antecedent infections. | this study was designed to investigate whether complex regional pain syndrome type i (crps i) could be linked to any previous infection. | 2001 | 11444711 |
| contribution of mn-cofactored superoxide dismutase (soda) to the virulence of streptococcus agalactiae. | superoxide dismutases convert superoxide anions to molecular oxygen and hydrogen peroxide, which, in turn, is metabolized by catalases and/or peroxidases. these enzymes constitute one of the major defense mechanisms of cells against oxidative stress and hence play a role in the pathogenesis of certain bacteria. we previously demonstrated that group b streptococci (gbs) possess a single mn-cofactored superoxide dismutase (soda). to analyze the role of this enzyme in the pathogenicity of gbs, we c ... | 2001 | 11447191 |
| identification and analysis of staphylococcus aureus components expressed by a model system of growth in serum. | a model system mimicking staphylococcus aureus bacteremia was developed by growth in serum under microaerobic conditions. eight genes induced by growth in serum were identified, including an antimicrobial peptide biosynthesis locus, amino acid biosynthetic loci, and genes encoding putative surface proteins. nine independent insertions were found in the major lysine biosynthesis operon, which encodes eight genes, is repressed by lysine in vitro, and is expressed in vivo. | 2001 | 11447207 |
| development of a ceue-based multiplex polymerase chain reaction (pcr) assay for direct detection and differentiation of campylobacter jejuni and campylobacter coli in thailand. | a novel ceue-based multiplex pcr system was developed as an efficient diagnostics test to detect and differentiate c. jejuni and c. coli. there is no cross reactivity between c. jejuni and c. coli. in addition, the assay does not produce a positive signal from other enteric bacteria including salmonella, shigella and escherichia coli strains. campylobacter detection sensitivity was determined to be equivalent to previously reported pcr for other enteric bacteria. we also noticed that silicon dio ... | 2001 | 11448558 |
| activity of bms284756 (t-3811) tested against anaerobic bacteria, campylobacter jejuni, helicobacter pylori and legionella spp. | bms284756, a novel des-fluoro (6) quinolone (formerly t-3811), was tested for activity and spectrum using reference agar dilution (ad) and etest (ab biodisk, solna, sweden) methods. the antimicrobial activities of bms284756, ciprofloxacin, gatifloxacin, levofloxacin, and trovafloxacin were evaluated against campylobacter jejuni (38 strains), helicobacter pylori (21 strains), legionella spp. (66 strains), and 197 anaerobic isolates. bms284756 (mic(90), 0.008 microg/ml) was four-fold more active t ... | 2001 | 11448563 |
| gt160-246, a toxin binding polymer for treatment of clostridium difficile colitis. | gt160-246, a high-molecular-weight soluble anionic polymer, was tested in vitro and in vivo for neutralization of clostridium difficile toxin a and b activities. five milligrams of gt160-246 per ml neutralized toxin-mediated inhibition of protein synthesis in vero cells induced by 5 ng of toxin a per ml or 1.25 ng of toxin b per ml. in ligated rat ileal loops, 1 mg of gt160-246 neutralized fluid accumulation caused by 5 microg of toxin a. at doses as high as 80 mg/loop, cholestyramine provided i ... | 2001 | 11451694 |
| rad54 protein stimulates the postsynaptic phase of rad51 protein-mediated dna strand exchange. | rad54 and rad51 are important proteins for the repair of double-stranded dna breaks by homologous recombination in eukaryotes. as previously shown, rad51 protein forms nucleoprotein filaments on single-stranded dna, and rad54 protein directly interacts with such filaments to enhance synapsis, the homologous pairing with a double-stranded dna partner. here we demonstrate that saccharomyces cerevisiae rad54 protein has an additional role in the postsynaptic phase of dna strand exchange by stimulat ... | 2001 | 11459988 |
| identification of the carbohydrate moieties and glycosylation motifs in campylobacter jejuni flagellin. | flagellins from three strains of campylobacter jejuni and one strain of campylobacter coli were shown to be extensively modified by glycosyl residues, imparting an approximate 6000-da shift from the molecular mass of the protein predicted from the dna sequence. tryptic peptides from c. jejuni 81-176 flagellin were subjected to capillary liquid chromatography-electrospray mass spectrometry with a high/low orifice stepping to identify peptide segments of aberrant masses together with their corresp ... | 2001 | 11461915 |
| more than one way to sense chemicals. | 2001 | 11466269 | |
| genome sequence and comparative analysis of the solvent-producing bacterium clostridium acetobutylicum. | the genome sequence of the solvent-producing bacterium clostridium acetobutylicum atcc 824 has been determined by the shotgun approach. the genome consists of a 3.94-mb chromosome and a 192-kb megaplasmid that contains the majority of genes responsible for solvent production. comparison of c. acetobutylicum to bacillus subtilis reveals significant local conservation of gene order, which has not been seen in comparisons of other genomes with similar, or, in some cases closer, phylogenetic proximi ... | 2001 | 11466286 |
| structure-function analysis of bfpb, a secretin-like protein encoded by the bundle-forming-pilus operon of enteropathogenic escherichia coli. | production of type iv bundle-forming pili by enteropathogenic escherichia coli (epec) requires bfpb, an outer-membrane lipoprotein and member of the secretin protein superfamily. bfpb was found to compose a ring-shaped, high-molecular-weight outer-membrane complex that is stable in 4% sodium dodecyl sulfate at temperatures of < or = 65 degrees c. chemical cross-linking and immunoprecipitation experiments disclosed that the bfpb multimeric complex interacts with bfpg, and mutational studies showe ... | 2001 | 11466288 |
| iron-dependent transcription of the frpb gene of helicobacter pylori is controlled by the fur repressor protein. | we have overexpressed and purified the helicobacter pylori fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake (frpb and ceue) and oxygen radical detoxification (kata and tsaa). dnase i footprint analysis showed that fur binds specifically to a high-affinity site overlapping the p(frpb) promoter and to low-affinity sites located upstream from promoters within both the frpb-kata and ceue-tsaa intergenic regions. construction of an isogen ... | 2001 | 11466300 |
| [molecular mimicry of bacterial polysaccharides and their role in etiology of infectious and autoimmune diseases]. | molecular mimicry is one of the most important pathogenic factor of microorganism and is defined as a structural similarity of microbial molecules to host tissue contributing to the pathogenicity. mimicry can be observed at the molecular, serological and functional level. in the review the infectious diseases have been discussed where the mimicry phenomenon may occur, and also autoimmune disease where due to the molecular mimicry bacterial structures are potent to induce adverse immune reactions ... | 2001 | 11468971 |
| assessment of the genetic diversity among strains of xanthomonas cynarae by randomly amplified polymorphic dna analysis and development of specific characterized amplified regions for the rapid identification of x. cynarae. | the randomly amplified polymorphic dna (rapd) method was used to investigate the genetic diversity in xanthomonas cynarae, which causes bacterial bract spot disease of artichoke. this rapd analysis was also intended to identify molecular markers characteristic of this species, in order to develop pcr-based markers which can be used to detect this pathogenic bacterium in artichoke fields. among the 340 rapd primers tested, 40 were selected on their ability to produce reproducible and reliable fin ... | 2001 | 11472907 |
| the ability of fla-typing schemes to discriminate between strains of campylobacter jejuni. | the aim of this investigation was to compare the usefulness of two previously published flagellin pcr-rflp typing (fla-typing) techniques for the subtyping of campylobacter jejuni strains, in terms of ease of use and discriminatory power. | 2001 | 11473586 |
| campylobacter contamination in french chicken production from farm to consumers. use of a pcr assay for detection and identification of campylobacter jejuni and camp. coli. | campylobacter contamination in french chicken production from the farm to the consumer was determined using a pcr assay for bacteria detection and identification. | 2001 | 11473590 |
| evaluation of methods for detection of toxins in specimens of feces submitted for diagnosis of clostridium difficile-associated diarrhea. | clostridium difficile is the principal pathogen associated with hospital-acquired acute diarrheal disease. we have evaluated the performances of six approaches for diagnosis of c. difficile-associated diarrhea (cdad). consecutive stool specimens (n = 200) from 133 patients were examined by cytotoxin assay, by culture of c. difficile on cycloserine-cefoxitin-fructose agar, and by toxin detection using four rapid immunoassay systems (oxoid toxin a test, immunocard toxin a test, techlab tox a/b ii ... | 2001 | 11474001 |
| relationship of campylobacter toxigenicity in vitro to the development of postinfectious irritable bowel syndrome. | campylobacter enteritis is associated with a significant risk of developing irritable bowel syndrome, but the mechanism is unknown. this study ascertained bowel symptoms in 93 patients 3 months after campylobacter jejuni enteritis infection. the infecting organisms were cultured, and the effects of culture supernatants on toxin-sensitive epithelial cell monolayers (hep-2, green monkey kidney epithelial [vero], and cho-k1) were investigated. in all, 50, 43, and 41 of the isolates showed toxigenic ... | 2001 | 11474430 |
| antimicrobial susceptibilities of campylobacter strains isolated from food animals in belgium. | campylobacter spp. are a frequent cause of diarrhoea in man, originating mostly from poultry. it has been suggested that the veterinary use of antibiotics is largely responsible for resistance in human isolates, particularly to quinolones. during a 6 month period from june to december 1998, 677 campylobacter isolates were obtained from healthy poultry and pigs. samples were taken at belgian slaughterhouses. species identification was performed by biochemical tests, multiplex pcr and sds-page of ... | 2001 | 11481294 |
| a genomic island in pseudomonas aeruginosa carries the determinants of flagellin glycosylation. | protein glycosylation has been long recognized as an important posttranslational modification process in eukaryotic cells. glycoproteins, predominantly secreted or surface localized, have also been identified in bacteria. we have identified a cluster of 14 genes, encoding the determinants of the flagellin glycosylation machinery in pseudomonas aeruginosa pak, which we called the flagellin glycosylation island. flagellin glycosylation can be detected only in bacteria expressing the a-type flagell ... | 2001 | 11481492 |
| occurrence of campylobacter jejuni in vegetables. | in order to understand the importance of vegetables in the transmission of thermophilic campylobacter, 56 samples of different vegetables were screened. out of these, 2 samples (1 spinach and 1 fenugreek) revealed the presence of campylobacter jejuni biotype i. both the isolates were enteropathogenic in rat ileal loop test. | 2001 | 11482564 |
| three patients with ophthalmoplegia associated with campylobacter jejuni. | cranial polyneuropathy is idiopathic in most patients. idiopathic cranial polyneuropathy is an acute postinfectious syndrome, along with guillain-barré syndrome and miller fisher syndrome, in which the common preceding pathogen is campylobacter jejuni. serum anti-gq1b antibodies are elevated in miller fisher syndrome and guillain-barré syndrome with ophthalmoplegia. three patients with idiopathic cranial polyneuropathy with predominant ocular involvement are presented. c. jejuni isolated from st ... | 2001 | 11483401 |
| identification of the helicobacter pylori anti-sigma28 factor. | flagellar motility is essential for colonization of the human gastric mucosa by helicobacter pylori. the flagellar filament is composed of two subunits, flaa and flab. transcription of the genes encoding these proteins is controlled by the sigma28 and sigma54 factors of rna polymerase respectively. the expression of flagellar genes is regulated, but no sigma28-specific effector was identified. it was also unclear whether h. pylori possessed a checkpoint for flagellar synthesis, and no gene encod ... | 2001 | 11489132 |
| characterizations of highly expressed genes of four fast-growing bacteria. | predicted highly expressed (phx) genes are characterized for the completely sequenced genomes of the four fast-growing bacteria escherichia coli, haemophilus influenzae, vibrio cholerae, and bacillus subtilis. our approach to ascertaining gene expression levels relates to codon usage differences among certain gene classes: the collection of all genes (average gene), the ensemble of ribosomal protein genes, major translation/transcription processing factors, and genes for polypeptides of chaperon ... | 2001 | 11489855 |
| characterization of haemophilus ducreyi cdta, cdtb, and cdtc mutants in in vitro and in vivo systems. | haemophilus ducreyi expresses a soluble cytolethal distending toxin (cdt) that is encoded by the cdtabc gene cluster and can be detected in culture supernatant fluid by its ability to kill hela cells. the cdta, cdtb, and cdtc genes of h. ducreyi were cloned independently into plasmid vectors, and their encoded proteins expressed singly or in various combinations in an escherichia coli background. all three gene products had to be expressed in order for e. coli-derived culture supernatant fluids ... | 2001 | 11500438 |
| cytolethal distending toxin demonstrates genotoxic activity in a yeast model. | cytolethal distending toxins (cdts) are multisubunit proteins produced by a variety of bacterial pathogens that cause enlargement, cell cycle arrest, and apoptosis in mammalian cells. while their function remains uncertain, recent studies suggest that they can act as intracellular dnases in mammalian cells. here we establish a novel yeast model for understanding cdt-associated disease. expression of the cdtb subunit in yeast causes a g2/m arrest, as seen in mammalian cells. cdtb toxicity is not ... | 2001 | 11500452 |
| cloning, expression, and catalytic activity of helicobacter hepaticus urease. | helicobacter hepaticus causes disease in the liver and lower intestinal tract of mice. it is strongly urease positive, although it does not live in an acidic environment. the h. hepaticus urease gene cluster was expressed in escherichia coli with and without coexpression of the helicobacter pylori nickel transporter nixa. as for h. pylori, it was difficult to obtain enzymatic activity from recombinant h. hepaticus urease; special conditions including nicl2 supplementation were required. the h. h ... | 2001 | 11500473 |
| demonstration of polysaccharide capsule in campylobacter jejuni using electron microscopy. | recently, we reported that campylobacter jejuni, an important gastrointestinal pathogen, has the genetic determinants to produce a capsular polysaccharide (karlyshev et al., mol. microbiol. 35:529-541, 2000). despite these data, the presence of a capsule in these bacteria has remained controversial. in this study we stain c. jejuni cells with the cationic dye alcian blue and demonstrate for the first time by electron microscopy that c. jejuni cells produce a polysaccharide capsule that is retain ... | 2001 | 11500474 |