Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| delivering human papillomavirus testing to the public. | 2004 | 14692823 | |
| detection of human papillomavirus in cervical lymph nodes: a highly effective strategy for localizing site of tumor origin. | patients with head and neck squamous cell carcinoma (hnscc) often come to clinical attention with a neck mass due to metastatic spread to lymph nodes. treatment is dictated by the subsequent determination of primary tumor site and stage. however, the primary site remains elusive in some patients even after an exhaustive examination. human papillomavirus type 16 (hpv-16) is an important etiologic agent for hnsccs that arise within the oropharynx but less so for tumors from nonoropharyngeal sites. ... | 2003 | 14695150 |
| notch1 can contribute to viral-induced transformation of primary human keratinocytes. | the human papillomavirus (hpv) is the most significant causative agent in the development of cervical cancer. despite its presence in almost all cervical cancers, hpv by itself is unable to transform a normal cell to a cancerous one. instead, additional cellular mutations are required to supplement the hpv oncoproteins e6 and e7. activation of the notch1 signaling pathway has been proposed as one of the cellular changes that cooperate with the e6 and e7 proteins to cause cervical cancers. this p ... | 2003 | 14695182 |
| geldanamycin and 17-allylamino-17-demethoxygeldanamycin potentiate the in vitro and in vivo radiation response of cervical tumor cells via the heat shock protein 90-mediated intracellular signaling and cytotoxicity. | ansamycin antibiotics inhibit function of the heat shock protein (hsp) 90, causing selective degradation of several intracellular proteins regulating such processes as proliferation, cell cycle regulation, and prosurvival signaling cascades. hsp90 has been identified previously as a molecular target for anticancer agents, including ionizing radiation (ir). therefore, we hypothesized that the ansamycin geldanamycin and its 17-allylamino-17-demethoxy analog (17-aag), which inhibit hsp90, would enh ... | 2003 | 14695217 |
| discovery of small-molecule inhibitors of the atpase activity of human papillomavirus e1 helicase. | the boehringer ingelheim compound collection was screened for inhibitors of the atpase activity of human papillomavirus e1 helicase to develop antiviral agents that inhibit human papillomavirus (hpv) dna replication. this screen led to the discovery of (biphenyl-4-sulfonyl)acetic acid 1, which inhibits the atpase activity of hpv type 6 e1 helicase with a low micromolar ic(50) value. a hit-to-lead exercise rapidly converted 1 into a low nanomolar lead series. | 2004 | 14695816 |
| transition to an irreversible state of senescence in hela cells arrested by repression of hpv e6 and e7 genes. | inhibition of human papillomavirus (hpv) e6 and e7 transcription by means of the e2 protein of bovine papillomavirus 1 (bpv1) has been shown to induce acute growth arrest in hpv-positive cervical carcinoma cells. this state of arrest is marked by the expression of senescence phenotypes including sa beta-gal activity and lipofuscin accumulation. in this study, we examined the reversibility of these phenotypes by exogenously expressing the e6 and e7 genes into hela cells growth-arrested by the dep ... | 2004 | 14706235 |
| a case-control study of endogenous hormones and cervical cancer. | both parity and oral contraceptive use are associated with elevated circulating levels of sex hormones, at least transiently, and with increased risk of cervical cancer in human papillomavirus (hpv)-infected women. we directly evaluated whether elevations in the physiologic levels of these hormones predispose to the development of cervical neoplasia. we identified 67 premenopausal and 43 postmenopausal women with cervical intraepithelial neoplasia 2, 3, or cervical cancer (>/=cin2) diagnosed dur ... | 2004 | 14710222 |
| analysis of the expression and localisation of a lap protein, human scribble, in the normal and neoplastic epithelium of uterine cervix. | recently, a lap protein, scribble, was identified in drosophila epithelia as a basolateral protein that controls the apical-basolateral polarity. loss of scribble causes disorganisation and overgrowth of the epithelia. scribble has a human homologue, human scribble (hscrib), which is a substrate of ubiquitin-mediated degradation by human papillomavirus e6 and the e6ap ubiquitin-protein ligase. in the present study, we revealed that hscrib localised to the basolateral regions of the epithelial ce ... | 2004 | 14710229 |
| molecular detection of infections associated with neoplasia. | this article discusses the molecular targets and the methods for identification of human papillomavirus and the human gammaherpesviruses, epstein-barr virus and human herpesvirus type 8. | 2003 | 14711098 |
| preventative and therapeutic vaccines for cervical cancer. | "high-risk" genotypes of the human papillomavirus (hpv), most commonly hpv genotype 16, are the primary etiologic agents of cervical cancer. indeed hpv dna is detected in 99% of cervical carcinomas. thus, cervical cancer and other hpv-associated malignancies might be prevented or treated by the induction of the appropriate viral-antigen-specific immune responses. transmission of papillomavirus may be prevented by the generation of antibodies to capsid proteins l1 and l2 that neutralize viral inf ... | 2003 | 14711335 |
| women's desired information about human papillomavirus. | as human papillomavirus (hpv) dna testing is incorporated into cervical carcinoma screening programs, educational messages must be developed to inform women's screening choices and manage psychosocial responses to hpv dna test results. however, little is known about women's questions and concerns about hpv or their attitudes toward hpv testing. | 2004 | 14716766 |
| mutation and aberrant expression of caveolin-1 in human oral squamous cell carcinomas and oral cancer cell lines. | caveolin-1, a tumor suppressor gene, was found to be inactivated by structural abnormalities or epigenetic changes in several types of human cancers. in order to investigate the role of caveolin-1 in the pathogenesis of human oral squamous cell carcinoma, the exon 1 and 3 sequences of the caveolin-1 were investigated in 74 oral squamous cell carcinomas and 15 oral cancer cell lines. in addition, caveolin-1 expression was examined in 15 oral cancer cell lines. mutations of the caveolin-1 gene wer ... | 2004 | 14719121 |
| e6/e7 proteins of hpv type 16 and erbb-2 cooperate to induce neoplastic transformation of primary normal oral epithelial cells. | head and neck squamous cell carcinomas (hnscc) are characterized by a marked propensity for local invasion and spread to cervical lymph nodes, with distant metastases developing in 30-40% of cases. hpv-16 is an important risk factor for hnscc. how hpv enhances susceptibility to hnscc is not fully understood, but seems to involve cofactors. in this study, we examined the effect of the cooperation between hpv-16 and the tyrosine kinase receptor erbb-2 on e-cadherin/catenin complex patterns and neo ... | 2004 | 14724563 |
| the expression of key cell cycle markers and presence of human papillomavirus in squamous cell carcinoma of the tonsil. | chemical carcinogens induce squamous cell carcinoma (scc) of the head and neck by targeting the p53 and the retinoblastoma (prb) pathways. human papillomavirus (hpv) might have an etiologic role in these cancers at particular sites. few studies have compared cell cycle protein expression in hpv-positive and hpv-negative tumors in this region. | 2004 | 14724900 |
| human papillomavirus (hpv) study of 2916 cytological samples by pcr and dna sequencing: genotype spectrum of patients from the west german area. | human papillomaviruses (hpvs) are aetiological agents for cervical cancer. more than 70 different hpv types that infect genital mucosa have been found. in order to develop a sensitive and specific detection and typing assay, a pcr/direct sequencing approach was used. two pairs of consensus primers were used for amplification of hpv dna and the pcr products obtained were analysed by automated sequencing. sequences were compared with those in genbank by using the blast program. in this study, 2916 ... | 2004 | 14729933 |
| risk factors for squamous intraepithelial lesions (sil) of the cervix among women residing at the us-mexico border. | it is now well established that cervical cancer is caused by oncogenic human papillomavirus (hpv) infections that commonly infect women worldwide. what remains to be understood are the factors that contribute to cervical cancer in the presence of hpv infection. we conducted a case-control analysis of women recruited at the us-mexico border to simultaneously evaluate factors associated with 3 cytologic outcomes: atypical squamous cells of undetermined significance or atypical glandular cells of u ... | 2004 | 14735476 |
| enhancement of suicidal dna vaccine potency by delaying suicidal dna-induced cell death. | dna-based alphaviral rna replicon vectors, also called suicidal dna vectors, alleviate the concerns of integration or transformation related to conventional dna vectors since suicidal dna vectors eventually cause apoptosis of transfected cells. however, the expression of inserted genes in these vectors is transient and the potency of suicidal dna vaccines may be compromised because of apoptotic cell death. therefore, to enhance the immune response to the human papillomavirus type 16 (hpv-16) e7 ... | 2004 | 14737094 |
| prospects for controlling cervical cancer at the turn of the century. | cervical cancer morbidity and mortality have decreased substantially during the last 50 years mostly due to successful organized or opportunistic screening with pap cytology in high and middle income countries. in many low income countries pap cytology screening is yet to be effectively implemented or has failed to reduce cervical cancer rates to an appreciable extent. the fact that infection with certain human papillomavirus (hpv) types is now recognized as a necessary cause of this disease has ... | 2003 | 14746030 |
| recruitment of replication protein a by the papillomavirus e1 protein and modulation by single-stranded dna. | with the exception of viral proteins e1 and e2, papillomaviruses depend heavily on host replication machinery for replication of their viral genome. e1 and e2 are known to recruit many of the necessary cellular replication factors to the viral origin of replication. previously, we reported a physical interaction between e1 and the major human single-stranded dna (ssdna)-binding protein, replication protein a (rpa). e1 was determined to bind to the 70-kda subunit of rpa, rpa70. in this study, usi ... | 2004 | 14747526 |
| atypical glandular cells of undetermined significance. outcome predictions based on human papillomavirus testing. | cases of atypical glandular cells (agc) diagnosed on liquid-based preparations were culled from a 3-year period. when available, residual cellular material was analyzed for human papillomavirus (hpv) by polymerase chain reaction and correlated with cytologic and histologic (biopsy) outcome. of 178,994 cytologic cases, 187 (0.1045%) contained agc compared with 8,740 (4.8828%) atypical squamous cells (asc) for an agc/asc ratio of 0.021. hpv results and follow-up were available for 108 specimens fr ... | 2004 | 14750245 |
| hypothesis: the act of taking a papanicolaou smear reduces the prevalence of human papillomavirus infection: a potential impact on the risk of cervical cancer. | based on data collected in a case-control study of hormonal contraceptive use and invasive cervical cancer among south african colored and black women, we have reported a five-fold reduction in incidence among women who in their lifetimes had previously received as few as two papanicolaou (pap) smears. given the conditions prevailing in the community under study, a reduction in risk of this magnitude was surprising. we hypothesized that the act of performing a pap smear may trigger an immune res ... | 2003 | 14750534 |
| the tea polyphenol, (-)-epigallocatechin gallate effects on growth, apoptosis, and telomerase activity in cervical cell lines. | to investigate the effect of the major tea polyphenol, (-)-epigallocatechin gallate (egcg) in cervical carcinogenesis. | 2004 | 14751158 |
| zyc101a for treatment of high-grade cervical intraepithelial neoplasia: a randomized controlled trial. | the objective of this study was to assess the safety and efficacy of a novel therapeutic, zyc101a, for the treatment of women with histologically confirmed cervical intraepithelial neoplasia (cin) 2/3. zyc101a contains plasmid-dna-encoding fragments derived from the e6 and e7 proteins of human papillomavirus (hpv) 16 and 18, and is formulated within small biodegradable microparticles. | 2004 | 14754702 |
| multicenter initiative seeking critical genes in respiratory papillomatosis. | to determine the host genes that govern susceptibility to recurrent respiratory papillomatosis (rrp). rrp is caused by human papillomavirus (hpv) 6 and 11. millions of babies are exposed during the birthing process, but relatively few develop the disease and the aggressiveness of the course is highly variable. genetically encoded host susceptibility is postulated. determining the host genes that govern susceptibility will enhance our understanding not only of rrp but also of host-viral interacti ... | 2004 | 14755217 |
| prevalence of papillomavirus infection in women in ibadan, nigeria: a population-based study. | to investigate the prevalence of and the risk factors for cervical infection with human papillomavirus (hpv) in an inner-city area of ibadan, nigeria, we interviewed and obtained a sample of cervical cells from 932 sexually active women aged 15 years or older. a total of 32 different hpv types were identified with an hpv prevalence of 26.3% overall and 24.8% among women without cervical lesions; or age-standardised to the world standard population of 28.3 and 27.3%, respectively. high-risk hpv t ... | 2004 | 14760378 |
| the multistage process of carcinogenesis in human esophageal epithelial cells induced by human papillomavirus. | to investigate the multistage process of carcinogenesis, the progressive alteration of the morphology, telomerase, cytogenesis, oncogenes and tumorigenicity in the process of immortalization and malignant transformation of the human fetal esophageal epithelial cell (shee) was studied. the shee cells were immortalized by gene e6e7 of human papilloma virus (hpv) type 18 in our laboratory and continually cultivated over 100 passages, which had been malignantly transformed. cells at the 11th, 35th, ... | 2004 | 14767516 |
| oral human papillomavirus infection in adults is associated with sexual behavior and hiv serostatus. | the prevalence and risk factors for oral human papillomavirus (hpv) infection are unknown, despite evidence for an etiological role for hpv in oral cancers. oral samples from human immunodeficiency virus (hiv)-seronegative (n=396) and hiv-seropositive (n=190) adults were tested for hpv dna. high-risk hpv infections were present in 2.1% of tonsil and 6.3% of oral-rinse specimens. the prevalence of oral high-risk hpv infection was greater in hiv-seropositive individuals (13.7% vs. 4.5%; p<.001). i ... | 2004 | 14767823 |
| screening for cervical cancer in developing countries. | cervical cancer is the most common malignancy amongst females in developing countries, mainly due to a lack of precursor screening. this absence of screening is the result of inherent disadvantages of the pap smear: high cost, low sensitivity, the need for a laboratory with high human expertise and a complex screening program logistic system. the prerequisites for screening in a developing country include a screening method that is affordable, which can be effectively applied once in a lifetime ... | 2004 | 14871510 |
| detection and typing of human papillomavirus in non-small cell lung cancer. | lung cancer is the most frequent cause of death in both men and women. smoking is the greatest risk factor for lung cancer and the relation of human papillomavirus (hpv) infection with lung cancer has been reported. hpv can be detected in small cell lung cancer samples with the methods like in situ hybridization, polymerase chain reaction (pcr), southern blotting, dot blotting. | 2004 | 14872117 |
| expression of human papillomavirus type 16 e6 and e7 oncoproteins in primary foreskin keratinocytes is sufficient to alter the expression of angiogenic factors. | human papillomavirus (hpv) 16 is involved in causing cervical cancer. the e6 and e7 proteins of hpv 16 immortalize human keratinocytes and this is due, at least in part, to inactivation of the tumor suppressor proteins p53 and prb. these tumor suppressor proteins also regulate the expression of pro- and antiangiogenic factors by cells. for this reason, experiments were conducted to determine whether the expression of e6 and e7 in primary keratinocytes alters the phenotype of these cells such tha ... | 2004 | 14968115 |
| liquid-based cytology findings of glassy cell carcinoma of the cervix. report of a case with histologic correlation and molecular analysis. | glassy cell carcinoma is a rare form of poorly differentiated carcinoma of the cervix with no obvious squamous or glandular differentiation. its liquid-based cytology findings have not been described before. | 2004 | 14969191 |
| virologic versus cytologic triage of women with equivocal pap smears: a meta-analysis of the accuracy to detect high-grade intraepithelial neoplasia. | the appropriate management of women with minor cytologic lesions in their cervix is unclear. we performed a meta-analysis to assess the accuracy of human papillomavirus (hpv) dna testing as an alternative to repeat cytology in women who had equivocal results on a previous pap smear. | 2004 | 14970277 |
| [low yield of hpv dna determination in the distal region of the male urethra]. | to assess the prevalence of human papillomavirus (hpv) deoxyribonucleic acid (dna), and of clinical and subclinical condilomatosis in men whose sex partners had been diagnosed with cervical intraepithelial neoplasia. | 2003 | 14974269 |
| polymorphism of transporter associated with antigen presentation 1 as a potential determinant for severity of disease in recurrent respiratory papillomatosis caused by human papillomavirus types 6 and 11. | recurrent respiratory papillomatosis (rrp) is a rare disease caused by human papillomaviruses (hpvs). it is characterized by multiple recurrences of benign neoplasms and has a variable clinical course, ranging from infrequent recurrence to acute airway obstruction. one way in which hpv subverts the immune system in rrp is by interfering with tap1 (transporter associated with antigen presentation 1). we examined whether a known tap1 polymorphism in the atpase domain altered the severity of diseas ... | 2004 | 14976605 |
| human papillomavirus and cervical cancer: issues for biobehavioral and psychosocial research. | there is now overwhelming evidence that high-risk, sexually transmitted types of human papillomavirus (hpv) are the main causal agent in cervical cancer. biobehavioral and psychosocial research is uniquely capable of addressing many of the issues raised by hpv and its link with cervical cancer. in this article we review current findings in this area and identify issues for future research. the first of the three sections explores issues associated with the introduction of hpv testing for the det ... | 2004 | 14979865 |
| genomic diversity of human papillomavirus-16, 18, 31, and 35 isolates in a mexican population and relationship to european, african, and native american variants. | cervical cancer, mainly caused by infection with human papillomaviruses (hpvs), is a major public health problem in mexico. during a study of the prevalence of hpv types in northeastern mexico, we identified, as expected from worldwide comparisons, hpv-16, 18, 31, and 35 as highly prevalent. it is well known that the genomes of hpv types differ geographically because of evolution linked to ethnic groups separated in prehistoric times. as hpv intra-type variation results in pathogenic differences ... | 2004 | 14980491 |
| prognostic significance of lymph node variables and human papillomavirus dna in invasive vulvar carcinoma. | the present study investigates the influence of lymph node pathological features and hpv dna status on the prognosis of vulvar invasive tumors. | 2004 | 14984953 |
| a phase ii trial of interleukin-12 in patients with advanced cervical cancer: clinical and immunologic correlates. eastern cooperative oncology group study e1e96. | the ability to mount lymphoproliferative responses to peptides derived from the human papillomavirus (hpv) e6 and e7 oncoproteins has been associated with regression of dysplastic lesions of the uterine cervix and loss of associated hpv infection. interleukin-12 (il-12) is a potent immunopotentiator of t-cell function, and has been shown in phase i clinical trials to be tolerable. | 2004 | 14984966 |
| antiretroviral therapy and the clinical evolution of human papillomavirus-associated genital lesions in hiv-positive women. | the effect of antiretroviral therapy on the natural history of human papillomavirus (hpv)-associated genital lesions was evaluated in 201 human immunodeficiency virus (hiv)-infected women who were followed-up for 1-6 years. gynecologic examinations were performed every 6-12 months. hpv sequences in cervico-vaginal cells, analyzed by polymerase chain reaction and typed by restriction fragment-length polymorphism analysis, were repeatedly detected in 126 women; 29 had transient hpv infection. geni ... | 2004 | 14986260 |
| cisplatin restores p53 function and enhances the radiosensitivity in hpv16 e6 containing siha cells. | most hpv-positive cervical cancer cells possess wild type p53 gene, but its normal p53 functions are disrupted by expression of hpvs e6. treatment with 0-20 microm cisplatin for 24 h in hpv16 e6 containing siha cells suppressed e6 mrna, reduced e6 protein, and restored p53 expression in dose-dependent manners. dual-parameter flow cytometric analysis indicated that sub-g(1) apoptotic cells, but not necrotic cells were the major species for cisplatin-induced cytotoxicity in siha cells. after 0-10 ... | 2004 | 14991767 |
| human papillomavirus genotyping by the dna chip in the cervical neoplasia. | human papillomavirus (hpv) is implicated as an etiologic agent in neoplasitc lesions of the cervix. in this study, we used an hpv dna chip to detect the type-specific sequence of hpv from cervical swabs in women with biopsy- proven neoplastic lesions of the cervix. four hundred seventy-one patients were involved and classified into four groups based on the cytopathologic diagnosis: group i (normal, n = 290), group ii (low-grade squamous intraepithelial lesions (sil), n = 68), group iii (high-gra ... | 2004 | 15000752 |
| cancer of the anal canal. | anal cancer is an uncommon tumour that represents 4% of all cancers of the lower gastrointestinal tract. its pathogenesis and treatment have undergone substantial reassessment over the past two decades, and this is likely to continue. anal cancer can be cured by synchronous chemoradiotherapy, a treatment that both enables anal continence to be retained and reserves abdominoperineal resection of the rectum and anal canal (with formation of a permanent colostomy) for recurrent or residual disease ... | 2004 | 15003197 |
| comparison of human papillomavirus dna levels in gynecological cancers: implication for cancer development. | we have previously demonstrated the presence of human papillomavirus (hpv) dna in several gynecological cancers using conventional pcr. in the present study, to further understand the role of hpv in malignant transformation of these cancers, the infection rates and viral loads of hpv 16 and 18 in gynecological cancers were analyzed using real-time quantitative pcr (qpcr). hpv 16 dna was detected in 61.0% (58/95), 15.2% (7/46) and 32.1% (18/56) of cases of cervical, endometrial and ovarian cancer ... | 2003 | 15004491 |
| the diagnostic utility of human papillomavirus-testing in combination with immunohistochemistry in advanced gynaecologic pelvic tumours: a new diagnostic approach. | gynaecologic pelvic tumours comprise a range of histological entities that are highly variable with respect to their clinical behaviour. the distinction of these tumours can be extremely difficult. accurate identification of the primary tumour site has significant impact on the treatment strategy and prognosis. in this study we investigated the diagnostic and clinical utility of hpv (human papillomavirus)-testing in combination with selected immunohistochemical markers in advanced pelvic tumours ... | 2004 | 15010819 |
| human papillomavirus detection: verification with cervical cytology. | thirteen specific types of human papillomavirus (hpv), classified as high-risk for the development of cervical cancer, have been reported in 99.7% of all cervical cancers. for this reason, and because of the reported lack of sensitivity of the papanicolaou (pap) smear for detecting hpv, some experts believe that the use of papillomavirus dna testing may replace cytology for routine gynecological screening. our goal was to validate a commercial assay, the digene hybrid capture-2 for the detection ... | 2004 | 15011974 |
| adults' knowledge and behaviors related to human papillomavirus infection. | our objective was to assess adults' knowledge, risk behaviors, and preference for information about human papillomavirus (hpv). | 2004 | 15014049 |
| simultaneous human papilloma virus type 16 e7 and cdk inhibitor p21 expression induces apoptosis and cathepsin b activation. | human papillomavirus type 16 (hpv-16) is the major risk factor for development of cervical cancer. the major oncoprotein e7 enhances cell growth control. however, e7 has in some reports been shown to induce apoptosis suggesting that there is a delicate balance between cell proliferation and induction of cell death. we have used the osteosarcoma cell line u2os cells provided with e7 and the cdk2 inhibitor p21 (cip1/waf1) under inducible control, as a model system for the analysis of e7-mediated a ... | 2004 | 15016552 |
| restriction fragment length polymorphism of l1 amplicon using rsa 1 detects five different human papillomavirus types and their co-infections among women attending a gynaecological outpatient department. | detection of human papillomavirus (hpv) types 6, 11, 16, 18, and 33 including co-infections among females attending gynaecological outpatient department and cancer clinics, was done by restriction fragment length polymorphism (using rsa-1), of approximately 450bp amplicon, obtained by the amplification of the l1 region of hpv genome with consensus primers my09/11 [cancer cells 7 (1989) 209]. the results were further tested with hpv type specific primers [j. med. virol. 29 (1989) 20]. the techniq ... | 2004 | 15019264 |
| adolescent sexually transmitted diseases: recent developments. | adolescents and young adults continue to have the highest rates of sexually transmitted diseases. new chlamydia and gonorrhea diagnostic tests are being used in innovative ways to increase the number of infections that are detected. nevertheless, challenges such as gonorrhea resistance and partner notification and treatment continue to hinder efforts to reduce the prevalence of these two bacterial infections. although recent surveillance data suggest a decreasing trend of herpes simplex virus 2 ... | 2004 | 15023277 |
| [expression of fascin 1 gene in the process of the immortalized esophageal carcinoma carcinogenesis]. | fascin 1 is the 55kda f-actin- binding cytoskeleton protein. fascin 1 gene was cloned from a human teratocarcinoma. up to now, the carcinogenesis mechanism of esophageal squamous cell carcinoma is unclear. the study was designed to identify the differentially expressed proteins and mrnas between the human immortalized esophageal epithelial cell line (shee) transfected by human papillomavirus type 18 e6e7 and the malignant transformation cell line (sheemt), which is derivated from shee, and to fu ... | 2004 | 15025950 |
| description of a seven-year prospective study of human papillomavirus infection and cervical neoplasia among 10000 women in guanacaste, costa rica,. | the guanacaste study ("guanacaste project," or gp), was designed to investigate the role of human papillomavirus (hpv) infection and its cofactors in the development of cervical neoplasia and to evaluate new cervical cancer screening technologies. the follow-up phase of the gp was designed to study why a small proportion of women infected with hpv develop cervical intraepithelial neoplasia grade 2 (cin 2), cin 3, or cancer (these three together are globally referred to as > or = cin 2, that is, ... | 2004 | 15030652 |
| anti human papillomavirus vaccine: the checkmate to human papillomavirus? | cervical cancer is the second most frequently found neoplasia in women worldwide. at least 95% of cervical cancers contain viral dna which, in 80% of cases, belongs to one of the human papillomavirus (hpv) viral types at high oncogenic risk: 16, 18, 31 and 45. hpv is, at this point, considered the first "necessary cause" of cervical cancer, against which primary prevention with a reduction in the risk of infection can be carried out. numerous molecular biological studies have been conducted to i ... | 2004 | 15032271 |
| resistance to apoptosis of hpv 16-infected laryngeal cancer cells is associated with decreased bak and increased bcl-2 expression. | human papillomavirus type 16 (hpv 16) plays an etiological role in human laryngeal carcinoma. apoptosis is closely associated with various biological processes including oncogenesis. this study investigated how hpv 16 oncoproteins e6 and e7 affect apoptosis in human laryngeal cancer cells. we established two human laryngeal cancer cell lines that expressed hpv 16 e6 and e7, respectively. using these two cell lines, we found that both e6 and e7 exhibited an inhibitive effect on apoptosis induced ... | 2004 | 15036664 |
| detection of human papillomavirus dna in sperm using polymerase chain reaction. | 2004 | 15037790 | |
| analysis of human papillomavirus prevalence and tp53 polymorphism in head and neck squamous cell carcinomas. | head and neck squamous cell carcinoma is a disease associated with tobacco and alcohol abuse. there is evidence that the oncogenic human papillomavirus (hpv) may also be a risk for upper aerodigestive tract cancers. high-risk hpvs encode two early proteins, e6 and e7, that can bind to p53 and prb, respectively, and induce its degradation or inactivation. the tp53 gene has a single polymorphism at codon 72 of exon 4 that encodes either arginine (arg) or proline (pro). the purpose of this study wa ... | 2004 | 15041222 |
| distinction of endocervical and endometrial adenocarcinomas: immunohistochemical p16 expression correlated with human papillomavirus (hpv) dna detection. | determining the origin of uterine adenocarcinomas can be difficult in biopsy and curettage specimens because the morphologic spectrum of endocervical and endometrial adenocarcinomas overlaps. in hysterectomy specimens, the primary site is often equivocal for tumors that involve the lower uterine segment and endocervix and lack identifiable precursor lesions. most endocervical adenocarcinomas (ecas) contain high-risk human papillomavirus (hpv) dna, whereas endometrial adenocarcinomas (emas) rarel ... | 2004 | 15043304 |
| prevalence of single and multiple hpv types in cervical carcinomas in jakarta, indonesia. | cervical cancer is the second most frequently occurring type of cancer in women worldwide. a persistent infection with high-risk human papillomavirus (hpv) is a necessary causal factor in cervical carcinogenesis. the distribution of hpv types in populations has been studied worldwide. in indonesia, however, few data are available describing the prevalence of hpv. cervical carcinoma is the most common female cancer in indonesia and causes high morbidity and mortality figures. with hpv vaccination ... | 2004 | 15047213 |
| nfi-ski interactions mediate transforming growth factor beta modulation of human papillomavirus type 16 early gene expression. | human papillomaviruses (hpvs) are present in virtually all cervical cancers. an important step in the development of malignant disease, including cervical cancer, involves a loss of sensitivity to transforming growth factor beta (tgf-beta). hpv type 16 (hpv16) early gene expression, including that of the e6 and e7 oncoprotein genes, is under the control of the upstream regulatory region (urr), and e6 and e7 expression in hpv16-immortalized human epithelial cells is inhibited at the transcription ... | 2004 | 15047811 |
| cervical smears and human papillomavirus typing in sex workers. | sex workers are at increased risk for sexually transmitted infections (sti), human papillomavirus (hpv) and hence cervical cancer. in belgium screening for cervical cancer starts at the age of 25, and is at 3 yearly intervals. the aim of this study is to assess risks for abnormal cervical smears and hpv in sex workers and decide whether the current screening policy is sufficient for them. | 2004 | 15054172 |
| detection of human papillomavirus from liquid-based cytology specimens by in-house pcr: a pilot study. | the papanicolaou smear remains the most common method for the detection of precancerous changes in cervical cytology. however, the introduction of a liquid-based cytology (lbc) technique expands the possibility of cervical intraepithelial neoplasia (cin) diagnosis, and permits detection of precancerous changes and human papillomavirus (hpv) simultaneously. in the pilot study reported here, using an in-house polymerase chain reaction (pcr) method, high-grade hpv was detected in 32% of a cohort of ... | 2004 | 15058739 |
| adding hpv16 testing to abnormal cervical smear detection is useful for predicting cin3: a prospective study. | the aim of this prospective study was to estimate whether adding human papillomavirus 16 (hpv16) testing to abnormal cervical smears is useful in the prediction of cervical intraepithelial neoplasia 3 (cin3). | 2004 | 15059166 |
| control of alpha subunit of eukaryotic translation initiation factor 2 (eif2 alpha) phosphorylation by the human papillomavirus type 18 e6 oncoprotein: implications for eif2 alpha-dependent gene expression and cell death. | phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eif2alpha) at serine 51 inhibits protein synthesis in cells subjected to various forms of stress including virus infection. the human papillomavirus (hpv) e6 oncoprotein contributes to virus-induced pathogenicity through multiple mechanisms including the inhibition of apoptosis and the blockade of interferon (ifn) action. we have investigated a possible functional relationship between the e6 oncoprotein and eif2a ... | 2004 | 15060162 |
| [detection of hpv16 e6 gene in cervical tissues by quantitative polymerase chain reaction]. | to establish a method for detection of human papillomavirus (hpv) type16 e6 gene in cervical carcinomas specimens. to study the relationship between the quantities of hpv16 e6 (human papillomavirus type16 e6 gene) in cervical tissues and the course of cervical disease in xinjiang. | 2004 | 15061964 |
| nmr probing of in silico identification of anti-hpv16 e7 mab linear peptide epitope. | a proteomics-based approach was exploited in order to individuate peptide sequences having the immunogenic potential to evoke humoral response. the epitope search utilized two parameters: the similarity level of the peptide sequence to the host's proteins, and the peptide capability to bind to the major histocompatibility complex class ii molecules. by this approach, the human papillomavirus 16 e7(49-63) rahynivtfcckcds peptide was individuated as the immunogenic epitope recognized by an anti-hp ... | 2004 | 15063005 |
| predictive significance of the alterations of p16ink4a, p14arf, p53, and proliferating cell nuclear antigen expression in the progression of cervical cancer. | the purpose of this research was to evaluate the clinical significance of p16ink4a, p14arf, p53, and proliferating cell nuclear antigen (pcna) expression in tumor progression of cervical cancer. | 2004 | 15073118 |
| the human papillomavirus 16 e6 protein binds to fas-associated death domain and protects cells from fas-triggered apoptosis. | high risk strains of human papillomavirus (hpv), such as hpv 16, cause human cervical carcinoma. the e6 protein of hpv 16 mediates the rapid degradation of the tumor suppressor p53, although this is not the only function of e6 and cannot completely explain its transforming potential. previous work in our laboratory has demonstrated that e6 can protect cells from tumor necrosis factor-induced apoptosis by binding to the c-terminal end of tumor necrosis factor r1, thus blocking apoptotic signal tr ... | 2004 | 15073179 |
| hdac inhibitors trigger apoptosis in hpv-positive cells by inducing the e2f-p73 pathway. | histone deacetylase (hdac) inhibitors induce an intrinsic type of apoptosis in human papillomavirus (hpv)-positive cells by disrupting the mitochondrial transmembrane potential (deltapsim). loss of deltapsim was only detected in e7, but not in e6 oncogene-expressing cells. hdac inhibition led to a time-dependent degradation of the pocket proteins prb, p107 and p130, releasing 'free' e2f-1 following initial g1 arrest. inhibition of proteasomal proteolysis, but not of caspase activity rescued prb ... | 2004 | 15077164 |
| human papillomavirus type 77 e6 protein selectively inhibits p53-dependent transcription of proapoptotic genes following uv-b irradiation. | dna damage, such as that elicited by uv-b, can induce either a cell cycle arrest or apoptosis that can be signalled by the p53 protein through the activation of a number of downstream cellular target genes. in contrast to oncogenic anogenital human papillomaviruses (hpvs), which mediate proteolytic degradation of p53, the e6 protein of cutaneous hpvs, such as hpv 77, do not promote p53 degradation. we have previously shown, however, that expression of hpv 77 e6 can effectively block uv-induced a ... | 2004 | 15077176 |
| telomere erosion and chromosomal instability in cells expressing the hpv oncogene 16e6. | progression to advanced-stage cervical carcinomas is characterized by a recurrent pattern of chromosomal rearrangements. structural chromosome rearrangements are generated through the fusion of broken chromosome ends. these chromosome breaks may be induced by mutagenic agents such as ionizing radiation, or chromosome ends may be exposed through extensive telomere shortening. the human papilloma virus oncogene 16e6 induces telomerase activity in human keratinocytes, a model system for cervical tu ... | 2004 | 15077181 |
| secretory heat-shock protein as a dendritic cell-targeting molecule: a new strategy to enhance the potency of genetic vaccines. | dna vaccines are an appealing strategy for inducing cytotoxic t-lymphocyte and antibody responses against tumor cells as well as infectious agents. dendritic cells (dcs) play a critical role in inducing immune responses, but their potential is not fully utilized in the dna vaccine setting since they take up only a minor fraction of the injected dna. here we describe a novel dna vaccination strategy based on the targeting of a modified tumor-associated antigen, the human papilloma virus (hpv) typ ... | 2004 | 15085173 |
| double cervix with bilateral and synchronous hsil associated with different high-risk hpv types. a case report. | high grade squamous intraepithelial lesion (hsil) of the cervix is well known to be associated with human papillomavirus (hpv) infection. hsil and invasive carcinomas occurring synchronously in genital malformations, such as a double cervix, have been reported. it has been postulated that the field effect phenomenon of hpv infection is responsible for this synchronous infection. however, there is no information in the literature on the specific types of hpv causing the concomitant lesions in cas ... | 2004 | 15085767 |
| are muslim women less susceptible to oncogenic human papillomavirus infection? a study from rural eastern india. | muslim women are known to have lower incidences of cervical cancer and/or human papillomavirus (hpv) infection. here we aim to determine any association that may be present between the oncogenic hpv16/18 infections and abnormal cytological lesions along with demographic and other attributes among indian muslim women (n = 478) and compare with the neighboring hindus (n = 534) from a prospective cohort study. agewise distribution of both subject-groups is similar. hpv16/18 infection is present in ... | 2004 | 15086729 |
| cpg-odn-stimulated dendritic cells act as a potent adjuvant for e7 protein delivery to induce antigen-specific antitumour immunity in a hpv 16 e7-associated animal tumour model. | we previously reported that both e7 and cpg-oligodeoxynucleotide (odn) are required for protecting animals from human papillomavirus (hpv) 16 e7-associated tumour challenge. here we investigate dendritic cells (dc)-based approach in this protection. in the study, we isolated bone marrow-derived dc and stimulated dc with e7 and odn. in vitro stimulation of dc with e7 plus odn resulted in more production of interleukin-12, as compared to that with e7 or odn alone. further injection with e7+odn-sti ... | 2004 | 15096191 |
| an inducible surface presentation system improves cellular immunity against human papillomavirus type 16 e7 antigen in mice after nasal administration with recombinant lactococci. | human papillomavirus type 16 (hpv-16) is the major causative agent of cervical cancer. to date, vaccine strategies against hpv-16 are based on the ability of the e7 oncoprotein to elicit an immune response against this virus. in this study, the use of an inducible or a constitutive system to produce the hpv-16 e7 protein in lactococcus lactis, a non-pathogenic and non-invasive gram-positive bacterium, was compared. the highest e7 production was obtained with the inducible system. when mice were ... | 2004 | 15096553 |
| performance assessment of eight high-throughput pcr assays for viral load quantitation of oncogenic hpv types. | infection with mucosotropic human papillomavirus (hpv) is the necessary cause of cervical intraepithelial neoplasia. several epidemiological studies suggest that hpv viral load can be a risk factor of cervical dysplasia. the purpose of the present study was to evaluate a methodology to determine hpv viral load of eight oncogenic hpv types (16, 18, 31, 39, 45, 51, 52, and 58). the quantitation assay is based on a high-throughput real-time pcr. the e6-e7 region of hpv types 16, 18, 45, and 51 were ... | 2004 | 15096567 |
| distribution of human papillomavirus type 16 variants in human immunodeficiency virus type 1-positive and -negative women. | the prevalence of human papillomavirus type 16 e6 variant lineages was characterized in a cross-sectional study of 24 human immunodeficiency virus type 1 (hiv)-positive and 33 hiv-negative women in new orleans. the european prototype was the predominant variant in the hiv-negative women (39.4 %), while in the hiv-positive women the european 350g variant was predominant (29.1 %). in exact logistic regression models, hiv-positive women were significantly more likely to harbour any variant with a n ... | 2004 | 15105540 |
| vulvar acanthosis with altered differentiation: a precursor to verrucous carcinoma? | verrucous carcinoma (vc) of the vulva is a rare variant of squamous cell carcinoma (scc) of the vulva that afflicts older women and is characterized by a well-differentiated morphology with minimal nuclear atypia. the pathogenesis of vc is uncertain and a putative role for human papillomavirus (hpv) is doubtful. we analyzed 9 vulvar vcs from 7 patients diagnosed as vc of the vulva over the past 10 years at brigham and women's hospital and beth israel deaconess medical center. the patients ranged ... | 2004 | 15105653 |
| anogenital human papillomavirus infection in males. | 2003 | 15122164 | |
| relationship between smoking and human papillomavirus infections in hiv-infected and -uninfected women. | background. smoking may increase the risk of cervical cancer, a disease that is related to human papillomavirus (hpv) infection. however, the effects of smoking on the natural history of hpv are poorly understood, especially in women coinfected with human immunodeficiency virus (hiv).methods. hiv-infected (n=1797) and hiv-uninfected (n=496) women were assessed every 6 months for type-specific hpv dna. smoking status was self-reported. covariates included age, parity, sexual behavior, hiv load, c ... | 2004 | 15122518 |
| role of the retinoblastoma pathway in senescence triggered by repression of the human papillomavirus e7 protein in cervical carcinoma cells. | repression of the endogenous human papillomavirus (hpv) type 18 e7 gene in hela cervical carcinoma cells by the bovine papillomavirus e2 transcription factor activates the retinoblastoma (rb) pathway and induces cells to undergo senescence. to determine whether activation of the rb pathway is responsible for senescence in response to hpv18 e7 repression, we tested the ability of wild-type and mutant e7 proteins to affect the activity of the rb pathway and to modulate senescence in these cells. e ... | 2004 | 15126344 |
| combined oral contraceptives and cervical cancer. | the issue of whether there might be an increased risk of cervical cancer associated with the use of oral contraceptives has been debated for decades. early studies found a modest association with long-term use. a literature review was performed over the past 3 years, to establish whether there is any new evidence linking cervical cancer with the use of oral contraceptives. | 2004 | 15128004 |
| immune cellular response to hpv: current concepts. | although cellular immunity is essential for the elimination of human papillomavirus (hpv), the mechanisms involved are still poorly understood. we summarize the main mechanisms involved in cellular immune response to infections caused by hpv. immunotherapies for hpv-related cancers require the disruption of t-cell response control mechanisms, associated with the stimulation of the th1 cytokine response. | 2004 | 15137933 |
| life style, environmental and genetic susceptibility to cervical cancer. | we have studied cervical cancer (cc) patients from venezuela and the us to elucidate the contribution of certain acquired and genetic susceptibility factors to the development of the disease. for acquired susceptibility factors, infection with high risk human papilloma virus (hpv), having multiple sex partners and having early sexual activities are significant risk factors for cc in venezuela. the latter two are not significant risk factors for the us population. infection with high risk hpv is ... | 2004 | 15138035 |
| failure of gamma interferon but not interleukin-10 expression in response to human papillomavirus type 11 e6 protein in respiratory papillomatosis. | recurrent respiratory papillomatosis (rrp) is a chronic, debilitating disease of the upper airway caused by human papillomavirus type 6 (hpv-6) or hpv-11. we describe responses of peripheral blood mononuclear cells (pbmc) and t cells from rrp patients and controls to the hpv-11 early proteins e6 and e7. pbmc were exposed in vitro to purified e6 or e7 proteins or transduced with fusion proteins containing the first 11 amino acids of the human immunodeficiency virus type 1 protein tat fused to e6 ... | 2004 | 15138179 |
| degradation of p53, not telomerase activation, by e6 is required for bypass of crisis and immortalization by human papillomavirus type 16 e6/e7. | bypass of two arrest points is essential in the process of cellular immortalization, one of the components of the transformation process. expression of human papillomavirus type 16 e6 and e7 together can escape both senescence and crisis, processes which normally limit the proliferative capacity of primary human keratinocytes. crisis is thought to be mediated by telomere shortening. because e6 stimulates telomerase activity and exogenous expression of the tert gene with e7 can immortalize kerati ... | 2004 | 15140967 |
| homogeneous real-time detection and quantification of nucleic acid amplification using restriction enzyme digestion. | a method for real-time fluorescent detection and quantification of nucleic acid amplification using a restriction endonuclease was developed. in this homogeneous system detection is mediated by a primer containing a reporter and quencher moiety at its 5' terminus separated by a short section of dna encoding a restriction enzyme recognition sequence. in the single stranded form, the signal from the fluorescent reporter is quenched due to fluorescence resonance energy transfer. however, as the pri ... | 2004 | 15144892 |
| human papillomavirus infection and cervical ectopy. | to investigate the prevalence of human papillomavirus (hpv), and hpv type 16 (hpv16) infection in cervical ectopy, and the presence of anti-hpv16 secretory iga (siga) antibodies. | 2004 | 15145262 |
| mapping and analysis of hpv16 integration sites in a head and neck cancer cell line. | human papillomavirus (hpv) is a circular double-stranded dna virus implicated in at least 90% of cervical and anogenital cancers and has been observed in approximately 20% of squamous cell carcinomas of the head and neck (scchn). transcription of the viral oncogenes e6 and e7 is regulated by expression of the e2 protein. disruption of the e2 gene sequence due to viral integration results in upregulation of e6 and e7, which promote tumorigenesis by abrogating p53 and prb functions. hpv integratio ... | 2004 | 15146560 |
| increased risk of skin cancer associated with the presence of epidermodysplasia verruciformis human papillomavirus types in normal skin. | human papillomaviruses (hpvs) are found in normal skin and in benign and malignant skin conditions. epidermodysplasia verruciformis (ev) hpv types are those most plausibly linked to the development of squamous cell carcinomas of the skin. | 2004 | 15149508 |
| [effects of anti-hpv16 e6-ribozyme on invasiveness of cervical carcinoma cell line caski and vascular endothelial growth factor expression]. | to investigate the changes in the invasiveness of cervical cancer cell line caski and the expression of vascular endothelial growth factor (vegf) in response to treatment with anti-hpv16 e6-ribozyme, which plays a important role in the malignant phenotype and conversion of cervical cancer cells. | 2004 | 15151824 |
| self-testing for human papillomavirus using a vaginal swab: placing prevention of cervical cancer in the patient's hands. | 2004 | 15151937 | |
| disease-stage variance in functional cd4(+) t-cell responses against novel pan-human leukocyte antigen-d region presented human papillomavirus-16 e7 epitopes. | given the anticipated clinical importance of helper and regulatory cd4(+) t cells reactive against human papillomavirus-16 e7 in the cervical carcinoma setting, we performed this study to identify novel e7-derived t helper (th) epitopes and to characterize functional anti-e7 th responses in normal donors and patients with cervical intraepithelial neoplasia i-iii or cervical cancer. candidate pan-hla-dr (d region) binding peptides were identified and synthesized based on results obtained using a ... | 2004 | 15161683 |
| analysis of aberrant dna methylation and human papillomavirus dna in cervicovaginal specimens to detect invasive cervical cancer and its precursors. | cancer of the uterine cervix is an important cause of death in women worldwide. pap smears as a tool for screening decreased the incidence and mortality of cervical cancer dramatically. this proof of principle study aimed to develop a potential tool for cervical screening using a test that can be applied by patients without visiting a physician and to increase the coverage rate, especially of the high-risk population with low socioeconomic status. | 2004 | 15161694 |
| an evaluation of liquid-based cytology and human papillomavirus testing within the uk cervical cancer screening programme. | the aim of this study is to evaluate different options for introducing liquid-based cytology (lbc) and human papillomavirus (hpv) testing into the uk cervical cancer screening programme. these include options that incorporate hpv testing either as a triage for mild and borderline smear abnormalities or as a primary screening test. outcomes include the predicted impact on resource use, total cost, life years and cost-effectiveness. extensive sensitivity analysis has been carried out to explore th ... | 2004 | 15162150 |
| identification of membrane proteins differentially expressed in human papillomavirus type 16 e5-transfected human keratinocytes by nanoelectrospray ionization mass spectrometry. | membrane proteins differentially expressed in human papillomavirus type 16 (hpv-16) e5-transfected hacat cells have been identified. membrane proteins were isolated and separated by two-dimensional gel electrophoresis. spots showing quantitative differences between e5-transfected and control cells were extracted and the proteins were identified by nanoelectrospray ionization mass spectrometry. a total of 24 spots was analysed. among the proteins showing differential expression, a decreased amoun ... | 2004 | 15166425 |
| effect of antiretroviral therapy on the incidence of genital warts and vulvar neoplasia among women with the human immunodeficiency virus. | the purpose of this study was to determine the incidence and predictors of genital warts and vulvar intraepithelial neoplasia among women with the human immunodeficiency virus. | 2004 | 15167825 |
| oral isotretinoin in the treatment of recalcitrant condylomata acuminata of the cervix: a randomised placebo controlled trial. | conventional therapies for human papillomavirus (hpv) infection are often associated with unsatisfactory response rates and high recurrence rates. the use of a systemic agent may more effectively control the virus. | 2004 | 15170007 |
| human papillomavirus and squamous cell carcinoma in a solitary tracheal papilloma. | we report the case of a 52-year-old woman suffering from breathlessness on exertion. a chest radiograph identified a lesion in the intrathoracic trachea. a tumor 0.4 x 0.5 x 0.7 cm in size causing an approximately 80% reduction in the cross-sectional area of the trachea 3 cm above the carina was removed, and histology showed moderately differentiated squamous cell carcinoma. intrathoracic resection of the tumor and anastomosis was performed through a right lateral thoracotomy. the final histolog ... | 2004 | 15172304 |
| systematic review of genomic integration sites of human papillomavirus genomes in epithelial dysplasia and invasive cancer of the female lower genital tract. | cancers of the anogenital tract as well as some head and neck cancers are caused by persistent infections with high-risk type human papillomaviruses (hpvs). two viral oncogenes, e6 and e7, induce severe chromosomal instability associated with centrosome aberrations, anaphase bridges, chromosome lagging, and breaking. this occurs early in preneoplastic lesions, when the viral genome still persists in an episomal state. in most invasive cancers and also in a few high-grade dysplastic lesions, howe ... | 2004 | 15172997 |
| identification of cytomegalovirus in a liquid-based gynecologic sample using morphology, immunohistochemistry, and dna real-time pcr detection. | the viral cytopathic effects of cytomegalovirus (cmv) are rarely encountered in conventional cervical vaginal smears and have never been reported in a liquid-based gynecologic sample (surepath). we present results of a liquid-based gynecologic sample (surepath) from an asymptomatic patient with classic cmv-associated granular or dense cyanophilic intracytoplasmic and intranuclear inclusion bodies with a clear surrounding zone. these inclusions were immunohistochemically positive for cmv. the pat ... | 2004 | 15176029 |