Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| determination of the mechanical properties of the skin of pig foetuses with respect to its structure. | skin is an important barrier protecting the organism against external environmental factors. determination of its mechanical characteristics as regards its structure has significant scientific and application value. in this work, uniaxial tensile tests were conducted to determine the basic mechanical parameters of skin with respect to its structure. the subject of the study were skin samples taken from domestic pig foetuses. they were excised from different parts of body, in the direction parall ... | 2011 | 21761809 |
| mesenchymal stem cells show radioresistance in vivo. | irradiation impacts on the viability and differentiation capacity of tissue-borne mesenchymal stem cells (msc), which play a pivotal role in bone regeneration. as a consequence of radiotherapy, bones may develop osteoradionecrosis. when irradiating human bone-derived msc in vitro with increasing doses, the cells' self-renewal capabilities were greatly reduced. mitotically stalled cells were still capable of differentiating into osteoblasts and preadipocytes. as a large animal model comparable to ... | 2011 | 21762375 |
| mitochondrial targeting of peroxiredoxin 5 is preserved from annelids to mammals but is absent in pig sus scrofa domesticus. | peroxiredoxin 5 (prdx5) is a thioredoxin peroxidase able to reduce hydrogen peroxide, alkyl hydroperoxides and peroxynitrite. in human, prdx5 was reported to be localized in the cytosol, the mitochondria, the peroxisomes and the nucleus. mitochondrial localization results from the presence of an n-terminal mitochondrial targeting sequence (mts). here, we examined the conservation of mitochondrial localization of prdx5 in animal species. we found that prdx5 mts is present and functional in the an ... | 2011 | 21763786 |
| molecular identification and phylogenetic analysis of trichinella isolates from different provinces in mainland china. | polymerase chain reaction (pcr) and sequencing are useful for species identification of trichinella spp., especially when their morphological characteristics useful for identifying taxa are lacking. in the present study, nine trichinella isolates from different provinces in mainland china were identified by the pcr-based method using the 5s ribosomal dna intergene spacer region (5s isr) and the mitochondrial large subunit ribosomal dna genes as molecular markers. the results indicated that eight ... | 2011 | 21773771 |
| prevalence of hepatitis e virus-specific antibodies in humans with occupational exposure to pigs. | due to the increasing number of non-travel-associated hepatitis e virus (hev) infections observed in several industrialised countries including germany, there is a substantial interest in the characterisation of risk factors and transmission routes relevant to autochthonous hev infections. autochthonous cases are believed to be the result of a zoonotic hev transmission from pigs, wild boars and deer. recently, a high prevalence of hev-specific antibodies in the german domestic pig population has ... | 2011 | 21773797 |
| mri studies of cryoinjury infarction in pig hearts: ii. effects of intrapericardial delivery of adipose-derived stem cells (adsc) embedded in agarose gel. | the purpose was to assess effects of intrapericardially deposited adipose-derived stem cells (adsc) as a source of angiogenic factors on cryoinjury infarction. to enhance this effect and reduce incorporation of adsc into tissue, the cells were immobilized in agarose gel patches transplanted onto cryoinjured epicardium. in domestic pigs (15-20?kg) the left ventricular (lv) anterior wall of exposed hearts was cryoinjured using aluminum rod (f=25?mm) cooled in liquid nitrogen. sterilized circular p ... | 2011 | 21774011 |
| cardiac aav9-s100a1 gene therapy rescues post-ischemic heart failure in a preclinical large animal model. | as a prerequisite for clinical application, we determined the long-term therapeutic effectiveness and safety of adeno-associated virus (aav)-s100a1 gene therapy in a preclinical large animal model of heart failure. s100a1, a positive inotropic regulator of myocardial contractility, becomes depleted in failing cardiomyocytes in humans and animals, and myocardial-targeted s100a1 gene transfer rescues cardiac contractile function by restoring sarcoplasmic reticulum calcium (ca(2+)) handling in acut ... | 2011 | 21775667 |
| vertebral growth modulation by hemicircumferential electrocoagulation: an experimental study in pigs. | introduction: this experimental study in pigs was aimed at evaluating spinal growth disorders after partial arrest of the vertebral epiphyseal plates (ep) and neurocentral cartilages (ncc). unilateral and multisegmental single or combined lesions of the physeal structures were performed by electrocogulation throughout a video-assisted thoracoscopical approach. materials and methods: thirty 4-week-old domestic pigs (mean weight 16 kg) were included in the experiments. the superior and inferior ep ... | 2011 | 21779856 |
| suppression of persistent atrial fibrillation by genetic knockdown of caspase 3: a pre-clinical pilot study. | aims atrial fibrillation (af) is linked to cardiomyocyte apoptosis, leading to atrial remodelling and reduction in electrical conduction velocity. we hypothesized that genetic suppression of an apoptotic key enzyme, caspase 3, would prevent the development of persistent af by reducing apoptosis which may serve as an arrhythmogenic substrate. methods and results atrial fibrillation was induced in domestic pigs by atrial burst pacing via an implanted cardiac pacemaker. study animals were then assi ... | 2011 | 21785105 |
| influence of tongue inspection during cysticercosis diagnosis on some behavioral and physiological stress measures in pigs (sus scrofa domestica). | to evaluate the level of stress induced by the tongue inspection method during cysticercosis diagnosis in pigs at 60 and 90 kg, 27 animals were assigned to one of three experimental groups. in the first group (t1), pigs were held with a snout noose. in addition, animals in t2 were subjected to tongue inspection, while in t0, none of the procedures described above were performed. resistance to be handled and serum cortisol concentration were higher (p?<?0.05) than those from t0 in t1 and t2 group ... | 2011 | 21786048 |
| porcine kobuvirus from pig stool specimens in shanghai, china. | in this study, a total of 116 stool specimens were collected from pigs of different ages in three pig farms of shanghai in china during 2010. forty-five (38.8%) stool specimens were positive for porcine kobuvirus using reverse transcriptase-polymerase chain reaction (rt-pcr). the prevalence rate of porcine kobuvirus in the three pig farms which are located in minhang district, qingpu district and fengxian district was 46.7%(21/45), 35.1%(13/37), and 32.4%(11/34), respectively. we demonstrated th ... | 2011 | 21789634 |
| comparison of anterior transgastric access techniques for natural orifice translumenal endoscopic surgery. | background: the advancement of natural orifice translumenal endoscopic surgery (notes) into clinical practice is dependent on its safety, efficacy, and efficiency. access is the obligatory first step in notes and serves as a surrogate to technical difficulties associated with this novel surgical approach. this study aimed to compare endoscopic transgastric access techniques in terms of safety, reproducibility, and efficiency. methods: seven variations for anterior transgastric notes access were ... | 2011 | 21789648 |
| catheter-based renal sympathetic denervation: chronic preclinical evidence for renal artery safety. | background: renal sympathetic hyperactivity is associated with hypertension, a leading cause of mortality worldwide. renal sympathetic denervation via the symplicity catheter system has been shown to decrease blood pressure by 33/11 mmhg by 6 months, with no radiofrequency (rf)-related adverse sequelae visible by ct/mr angiography or renal duplex ultrasound 6 months after the procedure. here, we present preclinical work predating those clinical results. we performed therapeutic renal sympathetic ... | 2011 | 21796327 |
| connexin 43 gene therapy prevents persistent atrial fibrillation in a porcine model. | aims atrial fibrillation (af) is the most common sustained cardiac arrhythmia, and effective treatment of af still remains an unmet medical need. af is associated with atrial conduction disturbances caused by electrical and/or structural remodeling. we hypothesized that af suppresses expression of the gap junction protein connexin 43, and that cx43 gene transfer to both atria would prevent persistent atrial fibrillation. the first aim of this study was to assess whether atrial fibrillation (af) ... | 2011 | 21799069 |
| african swine fever virus p72 genotype ix in domestic pigs, congo, 2009. | african swine fever virus p72 genotype ix, associated with outbreaks in eastern africa, is cocirculating in the republic of the congo with west african genotype i. data suggest that viruses from eastern africa are moving into western africa, increasing the threat of outbreaks caused by novel viruses in this region. | 2011 | 21801650 |
| the codon usage model of the context flanking each cleavage site in the polyprotein of foot-and-mouth disease virus. | to investigate the codon usage pattern of the contexts flanking 11 cleavage sites of foot-and-mouth disease virus (fmdv) polyprotein, the codon usage model of the corresponding codon position and the synonymous codon usage in the target contexts of 66 strains were characterized by two simple methods based on the relative synonymous codon usage value. the synonymous codons usage pattern was also compared between this virus and two species of hosts (cattle and domestic pig). it is indicated that f ... | 2011 | 21801856 |
| hepatitis e virus in domestic pigs and surface waters in slovenia: prevalence and molecular characterization of a novel genotype 3 lineage. | a novel hepatitis e virus (hev) genotype 3 lineage was identified in slovenian pig herds. stool samples from six slovenian pig farms were collected and tested for the presence of hev rna. of 85 individual samples 15 (20.3%) were positive for hev rna of which 2/38 (5.3%), 6/21 (28.6%) and 7/26 (26.9%) were from suckling, weanling and fattening pigs, respectively. additionally, 51 pooled porcine stool samples were tested in one of the biggest pig farm and the estimated infection rate of individual ... | 2011 | 21802527 |
| systemic gene delivery in large species for targeting spinal cord, brain, and peripheral tissues for pediatric disorders. | adeno-associated virus type 9 (aav9) is a powerful tool for delivering genes throughout the central nervous system (cns) following intravenous injection. preclinical results in pediatric models of spinal muscular atrophy (sma) and lysosomal storage disorders provide a compelling case for advancing aav9 to the clinic. an important translational step is to demonstrate efficient cns targeting in large animals at various ages. in the present study, we tested systemically injected aav9 in cynomolgus ... | 2011 | 21811247 |
| measurement of the midpalatal suture width. | abstract objective: to conduct a pilot study to investigate the potentiality to determine the midpalatal sutural width radiographically with a flat-panel volume computed tomography (fpvct) in a porcine model. materials and methods: bone samples from the midpalatal suture of five young (16 weeks) and five old (200 weeks) sus scrofa domestica were gathered. the midpalatal suture width was measured via fpvct and compared to respective histological preparations. results with p < .05 were considered ... | 2011 | 21812573 |
| hemodynamic and histologic characterization of a swine (sus scrofa domestica) model of chronic pulmonary arterial hypertension. | the purpose of this work was to develop and characterize an aortopulmonary shunt model of chronic pulmonary hypertension in swine and provide sequential hemodynamic, angiographic, and histologic data by using an experimental endoarterial biopsy catheter. nine yucatan female microswine (sus scrofa domestica) underwent surgical anastomosis of the left pulmonary artery to the descending aorta. sequential hemodynamic, angiographic, and pulmonary vascular samples were obtained. six pigs (mean weight, ... | 2011 | 21819696 |
| relative distribution of drugs in decomposed skeletal tissue. | skeletal tissues from a domestic pig exposed to amitriptyline, diazepam, and pentobarbital were analyzed to determine the relative distribution of these drugs in bone. following drug exposure and euthanasia, remains were allowed to decompose outdoors to complete skeletonization between summer 2007 and fall 2009. remains were recovered and separated according to bone type. twelve different bone types were pulverized and sampled in triplicate. each bone sample underwent methanolic extraction (96 h ... | 2010 | 21819797 |
| selection of reference genes for gene expression studies in pbmc from bama miniature pig under heat stress. | heat stress decreases immune function and increases disease susceptibility in stressed animals, which are important factors for industry and public health. we investigated the molecular mechanisms underlying heat stress by profiling the expression of target genes involved in the cellular response in the blood of bama miniature pigs (sus scrofa domestica) over 21 days with the use of quantitative rt-pcr (qrt-pcr). reliable standards were established for the normalization of qrt-pcr. six potential ... | 2011 | 21820186 |
| genetic detection and analysis of porcine bocavirus type 1 (pobov1) in european wild boar (sus scrofa). | novel porcine parvoviruses showing the genetic characteristics of bocaviruses have recently been identified. the first such porcine bocavirus (pobov1), described as boca-like virus (pbo-likev), was discovered in pmws affected pigs in sweden. later, several other bocaviruses with divergent genomes were reported under various names in domestic pigs. this is the first report of the presence of bocaviruses in european wild boars. 842 wild boar samples originating from the western region of romania ( ... | 2011 | 21822671 |
| influence of the halothane gene (hal) on pork quality in two commercial crossbreeds. | we evaluated the effect of the halothane (hal) gene on the quality of pork in domestic pigs. half-carcasses from two different commercial pig (sus domestica) crossbreeds were analyzed, 46 of which were homozygous dominant (hal(nn)) and 69 of which were heterozygous (hal(nn)) for the halothane gene. the measures included backfat thickness, lean meat percentage, carcass weight, ph 24 h after slaughtering, color, and drip loss; dna was extracted from the haunch muscle. swine with the hal(nn) genoty ... | 2011 | 21823098 |
| toxoplasma gondii in switzerland: a serosurvey based on meat juice analysis of slaughtered pigs, wild boar, sheep and cattle. | toxoplasmosis is one of the most important zoonotic diseases worldwide and is caused by the protozoan toxoplasma gondii. besides vertical infection during pregnancy, humans can get infected post-natally either by peroral uptake of sporulated toxoplasma oocysts or by ingestion of tissue cysts upon consumption of raw or undercooked meat. the aim of this study was to approximate the risk of human infection via meat consumption by estimating the seroprevalence of t. gondii in slaughtered animals in ... | 2011 | 21824348 |
| genome engineering with zinc-finger nucleases. | zinc-finger nucleases (zfns) are targetable dna cleavage reagents that have been adopted as gene-targeting tools. zfn-induced double-strand breaks are subject to cellular dna repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. this article briefly reviews the history of zfn development and summarizes applications that have been made to genome editing in many different organisms and situations. considerable progress has been made i ... | 2011 | 21828278 |
| feasibility of initiating extracorporeal life support during mechanical chest compression cpr: a porcine pilot study. | background: recently, portable extracorporeal membrane oxygenation (ecmo) machines have become commercially available. this creates the potential to utilize extracorporeal life support (ecls) for the treatment of sudden cardiac arrest in the emergency department, and potentially in the out-of-hospital setting. objective: we sought to determine the feasibility of installing the ecmo circuit during delivery of mechanical chest compression cpr. methods: we used 5 mixed-breed domestic swine with a m ... | 2011 | 21835144 |
| impact of epididymal maturation, ejaculation and in vitro capacitation on tyrosine phosphorylation patterns exhibited of boar (sus domesticus) spermatozoa. | mammalian spermatozoa acquire functionality during epididymal maturation and ability to penetrate and fertilize the oocyte during capacitation. the aim of this study was to investigate the impact of epididymal maturation, ejaculation and capacitation on phosphotyrosine content of sperm proteins. western blot, immunocytochemical and flow cytometry analyses demonstrated that epididymal maturation in vivo is associated with a progressive loss of phosphotyrosine residues of the sperm head followed b ... | 2011 | 21835448 |
| pseudorabies virus in wild swine: a global perspective. | suid herpesvirus 1 (suhv1, syn. aujeszky's disease virus [adv] or pseudorabies virus [prv]), which belongs to the family herpesviridae, subfamily alphaherpesvirinae, genus varicellovirus is the causative agent of aujeszky's disease (ad, pseudorabies), a notifiable disease, that causes substantial economic losses to the swine industry in countries, where ad is present. members of the family suidae (true pigs) are the only natural hosts for prv, although the virus can infect numerous other mammals ... | 2011 | 21837416 |
| neisseria meningitidis and escherichia coli are protected from leukocyte phagocytosis by binding to erythrocyte complement receptor 1 in human blood. | the initial interaction of gram-negative bacteria with erythrocytes and its implications on leukocyte phagocytosis and oxidative burst in human whole blood were examined. alexa-labeled escherichia coli, wild-type h44/76 n. meningitidis and the h44/76lpxa lipopolysaccharide (lps)-deficient mutant were incubated with whole blood using lepirudin as anticoagulant which has no adverse effects on complement. bacteria free in plasma, bound to erythrocytes or phagocytized by granulocytes and monocytes w ... | 2011 | 21839519 |
| cartographical analysis of african swine fever outbreaks in the territory of the russian federation and computer modeling of the basic reproduction ratio. | african swine fever (asf), have been introduced into the russian federation from transcaucasia countries, has spread widely across the territory of the southern region of russia since 2008. in this work we present an analysis of the spatial and temporal spread of the disease, determine risk factors by means of gis tools and model the dynamics of the epidemic process both within infected premises (farms) and at the between-farm level to estimate the basic reproduction ratio r(0). the analysis all ... | 2011 | 21840611 |
| Spatial distribution of Trichinella britovi, T. spiralis and T. pseudospiralis of domestic pigs and wild boars (Sus scrofa) in Hungary. | Trichinellosis is a foodborne disease caused by the consumption of raw meat and raw meat-derived products from swine, horse and some game animals infected with nematode worms of the genus Trichinella. Between June 2006 and February 2011, 16 million domestic pigs and 0.22 million wild boars (Sus scrofa) were tested for Trichinella sp. in Hungary. Trichinella infection was not found in any pigs slaughtered for public consumption. Nevertheless, Trichinella spiralis was detected in four backyard pig ... | 2012 | 21852037 |
| morphological changes and immunohistochemical expression of rage and its ligands in the sciatic nerve of hyperglycemic pig (sus scrofa). | the aim of our project was to study the effect of streptozotocin (stz)-induced hyperglycemia on sciatic nerve morphology, blood plasma markers and immunohistochemical expression of rage (the receptor for advanced glycation end-products), and its ligands-s100b and carboxymethyl lysine (cml)-advanced glycation endproduct (age) in the laboratory pig. six months after stz-injections, blood plasma measurements, morphometric analysis of sciatic nerve fiber density, immunofluorescent distribution of po ... | 2010 | 21852897 |
| clinically plausible hyperventilation does not exert adverse hemodynamic effects during cpr but markedly reduces end-tidal pco(2). | aims: ventilation at high respiratory rates is considered detrimental during cpr because it may increase intrathoracic pressure limiting venous return and forward blood flow generation. we examined whether ventilation at high, yet clinically plausible, tidal volumes could also be detrimental, and further examined effects on end-tidal pco(2) (p(et)co(2)). methods: sixteen domestic pigs were randomized to one of four ventilatory patterns representing two levels of respiratory rate (min(-1)) and tw ... | 2011 | 21854734 |
| CT fluoroscopy-guided placement of inferior vena cava filters: feasibility study in pigs. | To evaluate the feasibility of computed tomography (CT)-guided placement of inferior vena cava (IVC) filters in a swine model. | 2011 | 21856172 |
| comparison of short- and long-term results of drug-eluting vs. bare metal stenting in the porcine internal carotid artery. | to evaluate the development of neointimal hyperplasia after implantation of drug-eluting stents (paclitaxel) compared to bare metal stents in porcine internal carotid arteries (icas). | 2011 | 21861747 |
| Carrion beetles visiting pig carcasses during early spring in urban, forest and agricultural biotopes of Western Europe. | Carrion beetles are important in terrestrial ecosystems, consuming dead mammals and promoting the recycling of organic matter into ecosystems. Most forensic studies are focused on succession of Diptera while neglecting Coleoptera. So far, little information is available on carrion beetles postmortem colonization and decomposition process in temperate biogeoclimatic countries. These beetles are however part of the entomofaunal colonization of a dead body. Forensic entomologists need databases con ... | 2011 | 21867439 |
| occurrence of giardia and cryptosporidium in pigs on prince edward island, canada. | in a cross-sectional study of 633 pigs from 21 herds on prince edward island, canada (pei), the prevalence of infection with cryptosporidium and giardia, and the genotypes and species of isolates were determined in order to establish the zoonotic potential of pigs in this region. as determined by direct immunofluorescence microscopy (dfa), 18 herds (86%) and 163 animals (26%; 95% ci: 22-29%) tested positive for cryptosporidium, while just 3 herds (14%) and 6 animals (1%; 95% ci: 0.4-2%) tested p ... | 2011 | 21868167 |
| sensitive mie scattering immunoagglutination assay of porcine reproductive and respiratory syndrome virus (prrsv) from lung tissue samples in a microfluidic chip. | a microfluidic immunosensor utilizing mie scattering immunoaggultination assay was developed for rapid and sensitive detection of porcine reproductive and respiratory syndrome virus (prrsv) from lung tissue samples of domesticated pigs. antibodies against prrsv were conjugated to the surface of highly carboxylated polystyrene microparticles (diameter=920nm) and mixed with the diluted prrsv tissue samples in a y-shaped microchannel. antibody-antigen binding induced microparticle immunoagglutinati ... | 2011 | 21871925 |
| high prevalence of hepatitis e virus in french domestic pigs. | the importance of the domestic pig reservoir for hepatitis e virus (hev) was assessed by estimating the seroprevalence and prevalence of hev contaminated livers in french slaughter-aged pigs. 6565 sera and 3715 livers were randomly sampled from 186 pig farms throughout the country. taking the sampling design into account, the farm-level seroprevalence was 65% (95% ci 57-74) and 31% (95% ci 24-38) of the slaughter-aged pigs had antibodies against hev. the individual prevalence of hev rna positive ... | 2011 | 21872929 |
| enhanced endothelial progenitor cell angiogenic potency, present in early experimental renovascular hypertension, deteriorates with disease duration. | endothelial progenitor cells (epcs) augment neovascularization and repair of damaged tissues, but may undergo functional changes during exposure to cardiovascular risk factors. this study tested the hypothesis that early renovascular hypertension (rvh) modulates the temporal pattern of epc function that deteriorates with disease duration. | 2011 | 21873884 |
| Purification of tropomyosin, paramyosin, actin, tubulin, troponin and kinases for chemiproteomics and its application to different scientific fields. | p-aminobenzamidine (p-ABA) is used as a ligand in the purification of many serine proteases and in their removal from heterogeneous samples. Moreover, p-ABA has a potent ability to bind Ca(2+)-binding proteins. The binding ability and use of p-ABA in purification processes is still not fully understood. | 2011 | 21876731 |
| localization and neurochemical characteristics of the extrinsic sympathetic neurons projecting to the pylorus in the domestic pig. | the pylorus, an important part of the digestive tract controlling the flow of chyme between the stomach and the duodenum, is widely innervated by intrinsic and extrinsic nerves. to determine the locations of postganglionic sympathetic perikarya that innervate the pylorus of the domestic pig, a retrograde tracing method with application of fast blue tracer was used. all positive neuronal cell bodies (ca. 1750) were found in the celiac-cranial mesenteric ganglion complex (csmg), however, the coeli ... | 2011 | 21903161 |
| genetic suppression of atrial fibrillation using a dominant-negative ether-a-go-go-related gene mutant. | background: atrial fibrillation (af) is the most common sustained cardiac arrhythmia. gene therapy-dependent modulation of atrial electrophysiology may provide a more specific alternative to pharmacological and ablative treatment strategies. objective: we hypothesized that genetic inactivation of atrial repolarizing ether-a-go-go-related gene (erg) k(+) currents using a dominant-negative mutant would provide rhythm control in af. methods: ten domestic swine underwent pacemaker implantation and w ... | 2011 | 21907172 |
| pseudorabies virus infection in oklahoma hunting dogs. | pseudorabies is caused by suid herpesvirus 1, a member of the alphaherpesvirinae subfamily. although pigs are the natural host of pseudorabies virus (prv), the virus has a broad host range and may cause fatal encephalitis in many species. the united states obtained prv-free status in 2004 after the virus was eradicated from domestic swineherds, but the virus is still present in feral swine populations. the current report describes prv infection in 3 dogs that were used to hunt feral swine. the d ... | 2011 | 21908347 |
| burst strength testing of porcine intestinal anastomoses following negative pressure therapy. | the effect of negative pressure therapy (npt; the abthera™ open abdomen negative pressure therapy system, kci usa, inc., san antonio, tx) on the integrity of small intestinal anastomoses was evaluated using in situ burst strength testing in a domestic pig model. in each of 3 swine, 8 anastomoses were created, 4 using sutures and 4 using staples. after 24 hours of continuous npt, each anastomosis was subjected to burst strength testing in situ. mean ratios of burst strength of sutured anastomoses ... | 2011 | 21914702 |
| endovascular treatment of peripheral arterial injury with covered stents: an experimental study in pigs. | to evaluate the feasibility of using endovascular repair to treat penetrating arterial injuries with covered stents. feasibility was examined according to the circumferential extent of the injury. | 2011 | 21915495 |
| the first report on cryptosporidium suis and cryptosporidium pig genotype ii in eurasian wild boars (sus scrofa) (czech republic). | a total of 193 faecal samples of adult eurasian wild boars were collected at 12 enclosures across the czech republic and examined for cryptosporidium infection using both microscopic and molecular tools. cryptosporidium oocysts were not detected in any of the 193 faecal samples examined using the aniline-carbol-methyl violet staining method. thirty-two positive cases of cryptosporidium infection were detected using either genus- or species-specific nested pcr. mono-infection with cryptosporidium ... | 2011 | 21917378 |
| robust spinal motor neuron transduction following intrathecal delivery of aav9 in pigs. | adeno-associated viral vector 9 (aav9) has recently been shown to penetrate the blood-brain barrier via intravascular administration, making it a good candidate for diffuse gene delivery. however, the potential side effects of systemic delivery are unknown. intrathecal viral vector administration may be more invasive than intravenous injections, but it requires far less vector and it can be performed on an outpatient basis, making it an ideal route of delivery for clinical translation. a total o ... | 2011 | 21918551 |
| natural orifice transluminal endoscopic surgery (notes): assessment of peritoneal bacterial load after intraperitoneal antimicrobial wash and evaluation of hemodynamic changes in a porcine model. | abstract aims. natural orifice transluminal endoscopic surgery (notes) is a promising newly developed procedure; however, it is associated with many complications. the main aim of our study is to assess whether peritoneal wash with antibiotics decreases the bacterial load contamination related to the transgastric approach. methods. ten female farm pigs underwent transgastric peritoneoscopy with fallopian tubal ligation. five pigs were randomized to antibiotic wash of the peritoneal cavity and fi ... | 2011 | 21919809 |
| correlation of angiographic late loss with neointimal proliferation in stents evaluated by oct and histology in porcine coronary arteries. | we aimed to evaluate the correlation of angiographic late loss (ll) with the degree of in-stent neointimal proliferation assessed by optical coherence tomography (oct) and histology. | 2011 | 21920339 |
| differential decomposition patterns in charred versus un-charred remains. | although researchers have examined many aspects of fire modification, the rate and pattern of decomposition in charred remains have not been studied previously. this study utilized 48 domestic pigs, divided into 24 charred (head, neck, and limbs burned to crow-glassman level 1 and torso to level 2) and 24 un-charred pig carcasses. decomposition of control carcasses was scored at 50 accumulated degree days (add) intervals, and charred carcasses were also observed and photographed at this time. a ... | 2011 | 21923798 |
| molecular study of porcine circovirus type 2 circulating in portugal. | porcine circovirus type 2 (pcv2) belongs to the genus circovirus of the circoviridae family and has been associated with a disease named postweaning multisystemic wasting syndrome (pmws). in portugal the disease caused severe problems mainly between 1995 and 2003, but is nowadays less evident probably as the result of vaccination programmes initiated in 2007. this study reports the molecular analysis of 22 pcv2 strains obtained from domestic pigs originated from different regions of portugal. on ... | 2011 | 21925289 |
| preserved heart rate variability during therapeutic hypothermia correlated to 96 hours neurological outcomes and survival in a pig model of cardiac arrest. | objective:: therapeutic hypothermia initiated with cardiopulmonary resuscitation improves neurologic outcomes and survival after prolonged cardiac arrest. however, the potential mechanism by which hypothermia improves neurologic outcomes remains unclear. in the current study, we investigated the effect of rapid head cooling on 96-hr neurologic outcomes and survival by heart rate variability analysis in a pig model of prolonged cardiac arrest. design:: prospective randomized controlled animal stu ... | 2011 | 21926589 |
| tracking cats: problems with placing feline carnivores on δo, δd isoscapes. | several felids are endangered and threatened by the illegal wildlife trade. establishing geographic origin of tissues of endangered species is thus crucial for wildlife crime investigations and effective conservation strategies. as shown in other species, stable isotope analysis of hydrogen and oxygen in hair (δd(h), δ(18)o(h)) can be used as a tool for provenance determination. however, reliably predicting the spatial distribution of δd(h) and δ(18)o(h) requires confirmation from animal tissues ... | 2011 | 21931770 |
| Influence of castration-induced sex hormone deficiency on serum lipid levels and the genes expression in male pigs. | It has been known that the changes in gonadal steroids are closely associated with adipose tissue metabolism. Domestic pigs have been a well-recognized experimental animal in biomedical research because of their similarity to humans in body size and other physiological/anatomical features. The aims of this study were to investigate the influence of castration-induced sex hormone deficiency on serum lipid levels and the genes expression of key enzymes associated with lipogenic and lipolytic proce ... | 2011 | 21932170 |
| Seroprevalence of St-Valerien-like caliciviruses in Italian swine. | St-Valérien-like viruses are newly recognized porcine caliciviruses recently detected in North America and Europe. In this study, baculovirus-expressed virus-like particles of the St-Valérien strain 25A/ITA were generated and used for the development of an antibody-detection ELISA kit to assess the seroprevalence of these novel caliciviruses in swine. Antibodies specific for St-Valérien-like virus were detected in 63 (10.3?%) of 614 serum samples tested with titres ranging from 1?:?50 (28.6?%) t ... | 2012 | 21940412 |
| experimental infection of bama miniature pigs with a highly virulent classical swine fever virus. | currently, larger domestic pigs are only animals widely used in vaccine evaluation and pathogenicity study of classical swine fever virus (csfv). this study was aimed to create an alternative animal experimental infection model of csfv. | 2011 | 21943299 |
| Endoscopic full-thickness resection in the colon by using a clip-and-cut technique: an animal study. | Although endoscopic resection techniques have been established for definitive therapy of mucosal neoplasia, complete histopathological assessment or resection of subepithelial lesions is not reliably possible. | 2011 | 21944313 |
| optical properties of in situ eye lenses measured with x-ray talbot interferometry: a novel measure of growth processes. | the lens, a major optical component of the eye, has a gradient refractive index, which is required to provide sufficient refractive power and image quality. the refractive index variations across the lens are dependent on the distributions and concentrations of the varying protein classes. in this study, we present the first measurements of the refractive index in the in situ eye lens from five species using a specially constructed x-ray talbot grating interferometer. the measurements have been ... | 2011 | 21949870 |
| The evolutionary importance of cell ratio between notochordal and nucleus pulposus cells: an experimental 3-D co-culture study. | INTRODUCTION: Notochordal cells and nucleus pulposus cells are co-existing in the intervertebral disc at various ratios among different mammalians. This fact rises the question about the interactions and the evolutionary relevance of this phenomenon. It has been described that these relatively large notochordal cells are mainly dominant in early lifetime of all vertebrates and then differences occur with ageing. Human, cattle, sheep, and goat lose the cells with age, wherea ... | 2011 | 21953383 |
| myocardial bridges in domestic pig--morphological aspects. | the morphology of myocardial bridges (mb) in the heart of the domestic pig remain an open issue. despite numerous analyses of the subject, many controversies still exist. opinions also differ when the influence of the mb on haemodynamic processes in the coronal vessel system is concerned. in the examined group of 150 domestic pig's hearts, the length of the detected mb varied from 1.8 to 39.7 mm while their thickness amounted to 0.8 - 4.7 mm. both the longest and the thickest bridges were connec ... | 2011 | 21957735 |
| MRI study of cryoinjury infarction in pig hearts: i. Effects of intrapericardial delivery of bFGF/VEGF embedded in alginate beads. | The aim of the study was the testing of sustained intrapericardial delivery of vascular growth factors (GFs) from alginate beads on cryoinjury size and perfusion. In domestic pigs (15-20?kg, n?=?21), the left ventricular (LV) anterolateral wall of exposed hearts was cryoinjured using an aluminum rod (25?mm o.d.) cooled in liquid nitrogen. Alginate beads (d?=?3.2?±?0.2?mm), containing human recombinant basic fibroblast GF (bFGF, 50?µg) and vascular endothelial GF (VEGF, 50?µg)?+?heparin (50?µg) o ... | 2011 | 21960023 |
| pandemic a/h1n1/2009 influenza virus in swine, cameroon, 2010. | although swine origin a/h1n1/2009 influenza virus (hereafter "ph1n1″) has been detected in swine in 20 countries, there has been no published surveillance of the virus in african livestock. the objective of this study was to assess the circulation of influenza a viruses, including ph1n1 in swine in cameroon, central africa. we collected 108 nasal swabs and 98 sera samples from domestic pigs randomly sampled at 11 herds in villages and farms in cameroon. ph1n1 was isolated from two swine sampled ... | 2011 | 21963416 |
| ornithodoros moubata complement inhibitor is an equally effective c5 inhibitor in pigs and humans. | experimental evidence suggests that c inhibition and more particularly combined inhibition of c and the tlr coreceptor cd14 may be of therapeutic benefit in sepsis and other inflammatory conditions. a barrier to the testing and further development of many inhibitors is that their activity is species specific. pig is a relevant species for experimental models of human disease, and this study undertakes a comprehensive comparison of the inhibitory efficacy of the c5 inhibitor ornithodoros moubata ... | 2011 | 21964028 |
| antibodies to henipavirus or henipa-like viruses in domestic pigs in ghana, west africa. | henipaviruses, hendra virus (hev) and nipah virus (niv), have pteropid bats as their known natural reservoirs. antibodies against henipaviruses have been found in eidolon helvum, an old world fruit bat species, and henipavirus-like nucleic acid has been detected in faecal samples from e. helvum in ghana. the initial outbreak of niv in malaysia led to over 265 human encephalitis cases, including 105 deaths, with infected pigs acting as amplifier hosts for niv during the outbreak. we detected non- ... | 2011 | 21966471 |
| Development and evaluation of a selective medium for Brucella suis. | A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter- ... | 2011 | 21968103 |
| Tyrosine phosphorylation of lactate dehydrogenase A is important for NADH/NAD(+) redox homeostasis in cancer cells. | The Warburg effect describes an increase in aerobic glycolysis and enhanced lactate production in cancer cells. Lactate dehydrogenase A (LDH-A) regulates the last step of glycolysis that generates lactate and permits the regeneration of NAD(+). LDH-A gene expression is believed to be upregulated by both HIF and Myc in cancer cells to achieve increased lactate production. However, how oncogenic signals activate LDH-A to regulate cancer cell metabolism remains unclear. We found that the oncogenic ... | 2011 | 21969607 |
| Nitric oxide involvement in the acrosome reaction triggered by leptin in pig sperm. | Nitric oxide (NO) is a signaling molecule produced by intracellular nitric oxide synthase (NOS) enzymes. This free radical appears to affect sperm capacitation, a maturation step preceding acrosome reaction. Recent studies have reported leptin ability to promote capacitation and acrosome reaction in pig male gametes. | 2011 | 21970701 |
| vascular responses to drug-eluting and bare metal stents in diabetic/hypercholesterolemic and nonatherosclerotic porcine coronary arteries. | animal models used to gain insight into the vascular response to drug-eluting stents are generally juvenile and nonatherosclerotic, whereas stents are placed in patients with complex atherosclerosis and comorbidities. hence, models reflecting these complexities are needed to help elucidate the vascular effects of drug-eluting stents. we compared the vascular responses with bare metal stent (bms) and paclitaxel-eluting stent (pes) implantation in a diabetic/hypercholesterolemic (dm/hc) porcine mo ... | 2011 | 21972400 |
| reepithelialization of mid-dermal porcine burns after rapid enzymatic debridement with debrase®. | reepithelialization of mid-dermal burns is delayed by the presence of a layer of necrotic eschar. the authors hypothesized that rapid selective debridement using an enzymatic bromelain-based preparation, debrase®, would speed reepithelialization. forty mid-dermal burns (2.5 × 2.5 cm) were created on the back and flanks of two anesthetized domestic pigs (25 kg) using an aluminum bar (150 g) preheated in hot water (80°c) and applied for 20 seconds. the burns were randomized to a 4-hour topical app ... | 2011 | 21979853 |
| medicinal animals used in ethnoveterinary practices of the 'cariri paraibano', ne brazil. | zootherapy is important in various socio-cultural environments, and innumerous examples of the use of animal derived remedies can currently be found in many urban, semi-urban and more remote localities in all parts of the world, particularly in developing countries. however, although a number of ethnobiological inventories concerning the use of medicinal animals in human health care have been compiled in brazil in recent years, zootherapeutic practices in ethnoveterinary medicine (evm) are poorl ... | 2011 | 21985401 |
| reston ebolavirus in humans and animals in the philippines: a review. | the 2008 reston ebolavirus infection event in domestic pigs has triggered continuing epidemiologic investigations among philippine health and veterinary agencies in collaboration with international filovirus experts. prior to this, there were only 3 known and documented reston ebolavirus outbreaks in nonhuman primates in the world, all traced back to a single geographic source in the philippines in a monkey breeding/export facility. the first one in 1989 was the first-ever ebola virus that emerg ... | 2011 | 21987747 |
| assessment of rodents as animal models for reston ebolavirus. | the emergence of reston ebolavirus (rebov) in domestic swine in the philippines has caused a renewed interest in rebov pathogenicity. here, the use of different rodent species as animal disease models for rebov was investigated. balb/c and stat1(-)(/-) mice, hartley guinea pigs, and syrian hamsters were inoculated intraperitoneally with rebov strain pennsylvania or reston08-a. although virus replication occurred in guinea pigs, hamsters, and stat1(-/-) mice, progression to disease was only obser ... | 2011 | 21987777 |
| comparison of a lightweight polypropylene mesh (optilene® lp) and a large-pore knitted ptfe mesh (gore® infinit® mesh)-biocompatibility in a standardized endoscopic extraperitoneal hernia model. | purpose: the use of a mesh with good biocompatibility properties is of decisive importance for the avoidance of recurrences and chronic pain in endoscopic hernia repair surgery. as we know from numerous experiments and clinical experience, large-pore, lightweight polypropylene meshes possess the best biocompatibility. however, large-pore meshes of different polymers may be used as well and might be an alternative solution. me ... | 2011 | 21989559 |
| some aspects of purinergic signaling in the ventricular system of porcine brain. | numerous signaling pathways function in the brain ventricular system, including the most important - gabaergic, glutaminergic and dopaminergic signaling. purinergic signalization system - comprising nucleotide receptors, nucleotidases, atp and adenosine and their degradation products - are also present in the brain. however, the precise role of nucleotide signalling pathway in the ventricular system has been not elucidated so far. the aim of our research was the identification of all three eleme ... | 2011 | 21995888 |
| electromyographic thresholds after thoracic screw stimulation depend on the distance of the screw from the spinal cord and not on pedicle cortex integrity. | background context: present studies concerning the safety and reliability of neurophysiological monitoring during thoracic pedicle screw placement remain inconclusive, and therefore, universally validated threshold levels that confirm osseous breakage of the instrumented pedicles have not been properly established. purpose: the objective of this work was to analyze whether electromyographic (emg) thresholds, after stimulation of the thoracic pedicle screw, depend on the distance between the neur ... | 2011 | 21996524 |
| restricted enzooticity of hepatitis e virus genotypes 1 to 4 in the united states. | hepatitis e is recognized as a zoonosis, and swine are known reservoirs, but how broadly enzootic its causative agent, hepatitis e virus (hev), is remains controversial. to determine the prevalence of hev infection in animals, a serological assay with capability to detect anti-hev-antibody across a wide variety of animal species was devised. recombinant antigens comprising truncated capsid proteins generated from hev-subgenomic constructs that represent all four viral genotypes were used to capt ... | 2011 | 21998412 |
| Temperature-controlled two-wavelength laser soldering of tissues. | Laser tissue soldering is a method for bonding of incisions in tissues. A biological solder is spread over the cut, laser radiation heats the solder and the underlying cut edges and the incision is bonded. This method offers many advantages over conventional techniques (e.g., sutures). Past researches have shown that laser soldering, using a single laser, does not provide sufficient strength for bonding of cuts in thick (>1?mm) tissues. This study introduces a novel method for laser soldering of ... | 2011 | 22006733 |
| Managing tsetse transmitted trypanosomosis by insecticide treated nets--an affordable and sustainable method for resource poor pig farmers in Ghana. | An outbreak of tsetse-transmitted trypanosomiasis resulted in more than 50% losses of domestic pigs in the Eastern Region of Ghana (source: Veterinary Services, Accra; April 2007). In a control trial from May 4(th)-October 10(th) 2007, the efficacy of insecticide-treated mosquito fences to control tsetse was assessed. Two villages were selected--one serving as control with 14 pigsties and one experimental village where 24 pigsties were protected with insecticide treated mosquito fences. The 100 ... | 2011 | 22022625 |
| gastric endoscopic submucosal dissection assisted by a new traction method: the clip-band technique. a feasibility study in a porcine model (with video). | endoscopic submucosal dissection (esd) is the standard of care for treating gastric intramucosal neoplasias in japan. however, it is seldom performed in western countries, mainly because it is technically very challenging. several traction methods have been proposed to facilitate submucosal dissection, but they are usually not widely available or are difficult to apply. | 2011 | 22032320 |
| lactocrine programming of female reproductive tract development: environmental connections to the reproductive continuum. | for eutherian mammals a continuum of maternal support insures that development of progeny follows an optimal program. beginning in utero, such support extends into the early neonatal period when bioactive factors are communicated from mother to offspring in colostrum/milk. defined as lactocrine signaling, communication of milk-borne bioactive factors from mother to offspring as a consequence of nursing is important for development of somatic tissues, including the female reproductive tract (frt) ... | 2011 | 22033320 |
| analysis of clinical samples for early detection of classical swine fever during infection with low, moderate, and highly virulent strains in relation to the onset of clinical signs. | classical swine fever (csf) is a transboundary viral disease affecting swine. the clinical course of disease and the best diagnostic samples for early detection were examined using low, moderate, and highly virulent strains of csfv inoculated into 8-12 week old domestic pigs. clinical signs were monitored and recorded. nasal swabs, tonsil scrapings, blood and tonsils were tested using virus isolation, immunohistochemistry, and real-time reverse transcriptase pcr (rrt-pcr).severe clinical signs a ... | 2012 | 22036595 |
| Comparison of the pig and feline models for full thickness corneal transplantation. | The goal of this study was to report on the advantages and limitations of the pig and feline models for experimental in vivo corneal transplantation. | 2011 | 22050735 |
| fibre types in longissimus dorsi from wild and highly selected pig breeds. | longissimus dorsi muscles from six pig breeds were examined histologically. it was found that mean fibre diameter increased with degree of selection in domestic pigs and that the percentage of red fibres decreased-i.e. from mangulica and black slavonian to large white and swedish landrace. the percentage of white fibres showed the reverse tendency whilst that of the intermediate type was not markedly changed. in semi-wild pigs, the percentage of red fibres was still higher and in forest pigs the ... | 1981 | 22054604 |
| similar rates of chromosomal aberrant secondary oocytes in two indigenous cattle (bos taurus) breeds as determined by dual-color fish. | in vitro-matured metaphase ii (mii) oocytes with corresponding first polar bodies (i pb) from two indigenous cattle (bos taurus) breeds have been investigated to provide specific data upon the incidence of aneuploidy. a total of 165 and 140 in vitro-matured mii oocytes of the podolian (po) and maremmana (ma) breeds, respectively, were analyzed by fluorescence in situ hybridization using xcen and five chromosome-specific painting probes. oocytes with unreduced chromosome number were 13.3% and 6.4 ... | 2011 | 22056011 |
| Analysis of bacterial load and prevalence of mixed infections with Lawsonia intracellularis, Brachyspira hyodysenteriae and/or Brachyspira pilosicoli in German pigs with diarrhoea. | Lawsonia (L.) intracellularis, Brachyspira (B.) hyodysenteriae and B. pilosicoli are important pathogens in domestic pig production world-wide, responsible for porcine intestinal adenomatosis, swine dysentery, and porcine intestinal spirochetosis, respectively. Conventional PCR is the major diagnostic tool in the detection of the three pathogens, but the sole detection of bacterial DNA might lead to misinterpretations of results with respect to their clinical relevance, especially with mixed inf ... | 2011 | 22059295 |
| histochemical properties of fibre types in muscles of wild and domestic pigs and the effect of growth rate on muscle fibre properties. | this study reports the results of a comparison of the histochemical properties of various types of porcine muscles in wild and domestic pigs. in domestic pigs, the influence of growth rates on these properties were studied in five muscles: longissimus dorsi (ld), semimembranosus (sm), gluteus superficialis (gs), infra spinam (is) and masseter (mas). the growth rate was expressed as: (i) live weight (lw) at 165±2 days and (ii) daily lw gain from day 88 to day 165 (dg) on the experimental diet. ld ... | 2004 | 22061529 |
| differentiation of european wild boar (sus scrofa scrofa) and domestic swine (sus scrofa domestica) meats by pcr analysis targeting the mitochondrial d-loop and the nuclear melanocortin receptor 1 (mc1r) genes. | this work describes the differentiation of european wild boar (sus scrofa scrofa) and domestic swine (sus scrofa domestica) meats by pcr targeting sequences from two molecular markers: the mitochondrial displacement loop (d-loop) region and the nuclear melanocortin receptor 1 (mc1r) gene. a polymorphic d-loop fragment (~270bp) was amplified and sequenced in a number of wild and domestic sus scrofa meat samples, to find a nucleotide region suitable for pcr-rflp analysis. sequence data showed the ... | 2008 | 22062284 |
| Multi-locus analysis reveals a different pattern of genetic diversity for mitochondrial and nuclear DNA between wild and domestic pigs in East Asia. | A major reduction of genetic diversity in mtDNA occurred during the domestication of East Asian pigs. However, the extent to which genetic diversity has been lost in the nuclear genome is uncertain. To reveal levels and patterns of nucleotide diversity and to elucidate the genetic relationships and demographic history of domestic pigs and their ancestors, wild boars, we investigated 14 nuclear markers (including 8 functional genes, 2 pseudogenes and 4 intergenic regions) from 11 different chromo ... | 2011 | 22065995 |
| extensive polymorphism in the porcine toll-like receptor 10 gene. | the great importance of the toll-like receptors (tlrs) in innate immunity is well established, but one family member - tlr10 - remains elusive. tlr10 is expressed in various tissues in several species, but its ligand is not known and its function is still poorly understood. the open reading frame of tlr10 was sequenced in 15 wild boars, representing three populations, and in 15 unrelated domestic pigs of hampshire, landrace and large white origin. amino acid positions corresponding to detected n ... | 2011 | 22077964 |
| The design and recombinant protein expression of a consensus porcine interferon: CoPoIFN-a. | CoPoIFN-a is a recombinant non-naturally occurring porcine interferon-a (IFN-a). It was designed by scanning 17 porcine IFN-a nonallelic subtypes and assigning the most frequently occurring amino acid in each position. We used a porcine IFN-a (PoIFN-a) derived from domestic pig as a control. Both porcine IFN-a genes were introduced into yeast expression vector PpICZa-A and expressed in Pichia pastoris. The antiviral unit of these two IFN-as were assayed in MDBK, PK-15 and MARC-145 cells against ... | 2012 | 22088600 |
| comparative study between trimetazidine and ice slush hypothermia in protection against renal ischemia/reperfusion injury in a porcine model. | the aim of the study was to compare the effects of renal ice slush hypothermia and the use of trimetazidine in the protection against ischemia/reperfusion (i/r) injury. | 2011 | 22099266 |
| Protective effects of therapeutic hypothermia in post-resuscitation myocardium. | AIM OF THE STUDY: Post-resuscitation therapeutic hypothermia has been recommended because of its neuroprotective effects. However, a few studies have reported the effects of therapeutic hypothermia on the heart, especially in ventricular fibrillation cardiac arrest. The aim of this study was to determine whether therapeutic hypothermia attenuates post-resuscitation myocardial injury in a swine cardiac arrest model. METHODS: A prospective animal study was performed in the university hospital anim ... | 2011 | 22101201 |
| Free-ranging wild boar: a disease threat to domestic pigs in Switzerland? | The risk of transmission of pathogens from free-ranging wild boars (Sus scrofa scrofa) to outdoor domestic pigs (S. scrofa domesticus) is of increasing concern in many European countries. We assess this risk, using Switzerland as an example. We estimated 1) the prevalence of important pathogens in wild boars and 2) the risk of interactions between wild boars and outdoor pigs. First, we tested 252 wild boars from selected areas between 2008 and 2010 for infection with Brucella spp. Bacterial prev ... | 2011 | 22102657 |
| Hepatitis E virus genotype 3f in pigs in New Caledonia. | Hepatitis E virus (HEV) is highly prevalent in farm pigs worldwide and an increasing body of data from industrialised countries suggests that it is an agent of a porcine zoonosis. | 2011 | 22103949 |
| evolution of the porcine (sus scrofa domestica) immunoglobulin kappa locus through germline gene conversion. | immunoglobulin (ig) gene rearrangement and expression are central to disease resistance and health maintenance in animals. the ig kappa (igk) locus in swine (sus scrofa domestica) contributes to approximately half of all antibody molecules, in contrast to many other cetartiodactyla, whose members provide the majority of human dietary protein and in which kappa locus utilization is limited. the porcine igk variable locus is 27.9 kb upstream of five ig kappa j genes (igkj) which are separated from ... | 2011 | 22109540 |
| Osseointegration of SLActive implants in diabetic pigs. | OBJECTIVES: Diabetes mellitus is currently classified as a relative contraindication for implant treatment because of microangiopathies with the consequence of impaired bone regeneration and higher rates of implant failure. The study aim was to investigate peri-implant bone formation in a diabetic animal model in comparison to healthy animals and to evaluate the differences between conventional (SLA(®) ) and modified (SLActive(®) ) titanium implant surfaces on osseointegration. MATERIAL AND METH ... | 2011 | 22111960 |
| improved skin wound epithelialization by topical delivery of soluble factors from fibroblast aggregates. | introduction: timely coverage of an excised burn wound with a split-thickness skin graft, and efficient epithelialization at the donor site wound are key components in the treatment of burn patients. prompt healing is dependent on paracrine support from underlying dermal connective tissue fibroblasts. study aim: using the skin graft donor site in pig as a model for epithelialization, our aim was to evaluate if dermal signals, derived from cultured dermal fibroblast aggregates (finectra), can pro ... | 2011 | 22113100 |
| Impaired ß-adrenergic receptor signalling in post-resuscitation myocardial dysfunction. | OBJECTIVE: Post-resuscitation myocardial dysfunction is a major cause of fatality in patients receiving successful cardiopulmonary resuscitation. The mechanism of post-resuscitation myocardial dysfunction is largely unknown, although is generally considered related to ischaemia occurring during cardiac arrest and resuscitation and/or reperfusion injury after restoration of circulation. A key mechanism responsible for reduced contractile reserves in chronic heart failure is impaired ß-adrenergic ... | 2011 | 22115934 |