Publications

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de novo assembly and annotation of the asian tiger mosquito (aedes albopictus) repeatome with dnapipete from raw genomic reads and comparative analysis with the yellow fever mosquito (aedes aegypti).repetitive dna, including transposable elements (tes), is found throughout eukaryotic genomes. annotating and assembling the "repeatome" during genome-wide analysis often poses a challenge. to address this problem, we present dnapipete-a new bioinformatics pipeline that uses a sample of raw genomic reads. it produces precise estimates of repeated dna content and te consensus sequences, as well as the relative ages of te families. we shows that dnapipete performs well using very low coverage sequ ...201525767248
dr. phil's art corner: tiger mosaic and tiger mosaic close up. 201525793038
combined use of standard and throat microphones for measurement of acoustic voice parameters and voice categorization.the aim of the present study was to evaluate the reliability of the measurements of acoustic voice parameters obtained simultaneously using oral and contact (throat) microphones and to investigate utility of combined use of these microphones for voice categorization.201525795349
shrimp serine proteinase homologues pmmassph-1 and -2 play a role in the activation of the prophenoloxidase system.melanization mediated by the prophenoloxidase (propo) activating system is a rapid immune response used by invertebrates against intruding pathogens. several masquerade-like and serine proteinase homologues (sphs) have been demonstrated to play an essential role in propo activation in insects and crustaceans. in a previous study, we characterized the masquerade-like sph, pmmassph1, in the black tiger shrimp penaeus monodon as a multifunctional immune protein based on its recognition and antimicr ...201525803442
asian tigers, pacific panthers. 201525806699
common misconceptions about back pain in sport: tiger woods' case brings five fundamental questions into sharp focus. 201525807161
shifting elasmobranch community assemblage at cocos island--an isolated marine protected area.fishing pressure has increased the extinction risk of many elasmobranch (shark and ray) species. although many countries have established no-take marine reserves, a paucity of monitoring data means it is still unclear if reserves are effectively protecting these species. we examined data collected by a small group of divers over the past 21 years at one of the world's oldest marine protected areas (mpas), cocos island national park, costa rica. we used mixed effects models to determine trends in ...201525807991
identification and characterization of diverse groups of endogenous retroviruses in felids.endogenous retroviruses (ervs) are genetic elements with a retroviral origin that are integrated into vertebrate genomes. in felids (mammalia, carnivora, felidae), ervs have been described mostly in the domestic cat, and only rarely in wild species. to gain insight into the origins and evolutionary dynamics of endogenous retroviruses in felids, we have identified and characterized partial pro/pol erv sequences from eight neotropical wild cat species, belonging to three distinct lineages of felid ...201525808580
eye of the tiger sign in a 23 year patient with mitochondrial membrane protein associated neurodegeneration. 201525819119
stuart lipton: ufos and flying tigers. 201525819482
sleep and the use of energy products in a combat environment.the use of energy products appears to be widespread among deployed personnel, presumably to combat fatigue and sleep deprivation. however, these products have been associated with unpleasant side effects and adverse events, including insomnia, mood swings, fatigue, cardiac arrest, and even death.201525830795
vertical oviposition preferences of the asian tiger mosquito, aedes albopictus, in temperate north america.as a hyperaggressive mosquito that is also a public health threat, aedes albopictus (skuse), the asian tiger mosquito, is a major priority for control efforts. we examine one aspect of ae. albopictus biology: oviposition height. field-based research in an urban habitat was conducted to determine if a height preference exists for this species. larval and egg counts showed a significant preference for oviposition at ground level (0 m) compared to heights of 1, 2, 3, or 4 m (p < 0.01). an experimen ...201425843091
evaluation of bg-sentinel trap as a management tool to reduce aedes albopictus nuisance in an urban environment in italy.since its introduction and establishment in italy during the early 1990s, the asian tiger mosquito, aedes albopictus, has spread over large parts of italy and other mediterranean countries. aedes albopictus is both a nuisance and a competent vector for various arthropod-borne pathogens. although efficient traps for ae. albopictus exist and are used for population monitoring, their use as a control tool has not yet been studied. we evaluated biogents bg-sentinel mosquito traps, used with the bg l ...201525843172
updated distribution of aedes albopictus in oklahoma, and implications in arbovirus transmission.a series of statewide surveys were conducted in oklahoma in the summers between 1991 and 2004 to identify the distribution of aedes albopictus. adult mosquitoes were identified in 63 counties, bringing the currently known distribution of ae. albopictus in the state to 69 of 77 counties. the widespread presence of ae. albopictus in oklahoma has important current and future public and veterinary health implications for surveillance and control efforts.201525843181
a novel biosurfactant produced by aureobasidium pullulans l3-gpy from a tiger lily wild flower, lilium lancifolium thunb.yeast biosurfactants are important biotechnological products in the food industry, and they have medical and cosmeceutical applications owing to their specific modes of action, low toxicity, and applicability. thus, we have isolated and examined biosurfactant-producing yeast for various industrial and medical applications. a rapid and simple method was developed to screen biosurfactant-producing yeasts for high production of eco-friendly biosurfactants. using this method, several potential niche ...201525849549
landscape-scale accessibility of livestock to tigers: implications of spatial grain for modeling predation risk to mitigate human-carnivore conflict.innovative conservation tools are greatly needed to reduce livelihood losses and wildlife declines resulting from human-carnivore conflict. spatial risk modeling is an emerging method for assessing the spatial patterns of predator-prey interactions, with applications for mitigating carnivore attacks on livestock. large carnivores that ambush prey attack and kill over small areas, requiring models at fine spatial grains to predict livestock depredation hot spots. to detect the best resolution for ...201525859339
serum biochemical reference intervals for free-living sand tiger sharks (carcharias taurus) from east australian waters.sharks are top-order predators in ocean food chains and the star attractions in aquaria worldwide. unfortunately, blood biochemistry reference intervals (ri) have been determined for few species.201525865808
selecting representative microsatellite loci for genetic monitoring and analyzing genetic structure of an outbred population of orange tabby cats in china.we optimized a panel of microsatellite markers from cat and tiger genetic data for efficient genetic monitoring and used it to analyze the genetic structure of an outbred cat stock in china. we selected a set of rich polymorphic microsatellite loci from 131 cat microsatellite loci and 3 sumatran tiger microsatellite loci using agarose gel electrophoresis. next, the set of optimized genetic markers was used to analyze the genetic variation in an outbred population of orange tabby cats in china by ...201525867323
lambl's excrescence, migrainous headaches, and "tiger stripes": puzzling findings in one patient.lambl's excrescences are mobile, thin, filiform structures that occur at sites of valve closure. even though many clinicians consider them to be part of the normal aging process, evidence suggests an association between lambl's excrescences and ischemic stroke, migrainous headaches, and acute coronary syndromes. as a thin filamentous structure, a lambl's excrescence is better detected and characterized with transesophageal than with transthoracic echocardiography. intracardiac oscillatory struct ...201525873805
do cryptic species exist in hoplobatrachus rugulosus? an examination using four nuclear genes, the cyt b gene and the complete mt genome.he chinese tiger frog hoplobatrachus rugulosus is widely distributed in southern china, malaysia, myanmar, thailand, and vietnam. it is listed in appendix ii of cites as the only class ii nationally-protected frog in china. the bred tiger frog known as the thailand tiger frog, is also identified as h. rugulosus. our analysis of the cyt b gene showed high genetic divergence (13.8%) between wild and bred samples of tiger frog. unexpected genetic divergence of the complete mt genome (14.0%) was als ...201525875761
folate and folic acid in the periconceptional period: recommendations from official health organizations in thirty-six countries worldwide and who.to summarize the recommendations on folate intake and folic acid supplementation and fortification in the periconceptional period, aimed at prevention of neural tube defects (ntd), provided by official health organizations in different countries worldwide and who.201625877429
strategies of a thirteen year surveillance programme on aedes albopictus (stegomyia albopicta) in southern switzerland.in ticino, a canton located south of the alps in switzerland, a surveillance programme on aedes albopictus (stegomyia albopicta) started in 2000 seeing that the region was considered at high risk of introduction based on the rapid spread of this mosquito in neighbouring italy.201525890173
molecular markers for analyses of intraspecific genetic diversity in the asian tiger mosquito, aedes albopictus.the dramatic worldwide expansion of aedes albopictus (the asian tiger mosquito) and its vector competence for numerous arboviruses represent a growing threat to public health security. molecular markers are crucially needed for tracking the rapid spread of this mosquito and to obtain a deeper knowledge of population structure. this is a fundamental requirement for the development of strict monitoring protocols and for the improvement of sustainable control measures.201525890257
tigers of sundarbans in india: is the population a separate conservation unit?the sundarbans tiger inhabits a unique mangrove habitat and are morphologically distinct from the recognized tiger subspecies in terms of skull morphometrics and body size. thus, there is an urgent need to assess their ecological and genetic distinctiveness and determine if sundarbans tigers should be defined and managed as separate conservation unit. we utilized nine microsatellites and 3 kb from four mitochondrial dna (mtdna) genes to estimate genetic variability, population structure, demogra ...201425919139
native wolbachia from aedes albopictus blocks chikungunya virus infection in cellulo.wolbachia, a widespread endosymbiont of terrestrial arthropods, can protect its host against viral and parasitic infections, a phenotype called "pathogen blocking". however, in some cases wolbachia may have no effect or even enhance pathogen infection, depending on the host-wolbachia-pathogen combination. the tiger mosquito aedes albopictus is naturally infected by two strains of wolbachia, walba and walbb, and is a competent vector for different arboviruses such as dengue virus (denv) and chiku ...201525923352
displacement, deprivation and development: the impact of relocation on income and livelihood of tribes in similipal tiger and biosphere reserve, india.a large volume of literature describes adverse consequences of conservation-induced displacement on indigenous communities depended on natural resources of wildlife habitat. resettlement policies in protected areas the world over are mainly designed and implemented without consideration of social and economic costs of exclusion. this study examined income and poverty profile of tribal residents in similipal tiger and biosphere reserve in india, relative to the households relocated out of the res ...201525931297
ethnomedicinal uses, pharmacological activities, and cultivation of lignosus spp. (tiger׳s milk mushrooms) in malaysia - a review.several members of the genus lignosus, which are collectively known as cendawan susu rimau (in malay) or tiger׳s milk mushrooms (tmm), are regarded as important local medicine particularly by the indigenous communities in malaysia. the mushroom sclerotia are purportedly effective in treating cancer, coughs, asthma, fever, and other ailments. the most commonly encountered lignosus spp. in malaysia was authenticated as lignosus rhinocerotis (cooke) ryvarden (synonym: polyporus rhinocerus), which i ...201525937256
a comparison of food habits and prey preference of amur tiger (panthera tigris altaica) at three sites in the russian far east.prey availability is one of the principal drivers of tiger distribution and abundance. therefore, formulating effective conservation strategies requires a clear understanding of tiger diet. we used scat analysis in combination with data on the abundance of several prey species to estimate amur tiger diet and preference at 3 sites in the russian far east. we also examined the effect of pseudoreplication on estimates of tiger diet. we collected 770 scats across the 3 sites. similar to previous stu ...201525939758
canine distemper virus as a threat to wild tigers in russia and across their range.canine distemper virus (cdv) has recently been identified in populations of wild tigers in russia and india. tiger populations are generally too small to maintain cdv for long periods, but are at risk of infections arising from more abundant susceptible hosts that constitute a reservoir of infection. because cdv is an additive mortality factor, it could represent a significant threat to small, isolated tiger populations. in russia, cdv was associated with the deaths of tigers in 2004 and 2010, a ...201525939829
teaching braille letters, numerals, punctuation, and contractions to sighted individuals.braille-character recognition is one of the foundational skills required for teachers of braille. prior research has evaluated computer programming for teaching braille-to-print letter relations (e.g., scheithauer & tiger, 2012). in the current study, we developed a program (the visual braille trainer) to teach not only letters but also numerals, punctuation, symbols, and contractions; we evaluated this program with 4 sighted undergraduate participants. exposure to this program resulted in maste ...201525943465
genetic variation, structure, and gene flow in a sloth bear (melursus ursinus) meta-population in the satpura-maikal landscape of central india.sloth bears (melursus ursinus) are endemic to the indian subcontinent. as a result of continued habitat loss and degradation over the past century, sloth bear populations have been in steady decline and now exist only in isolated or fragmented habitat across the entire range. we investigated the genetic connectivity of the sloth bear meta-population in five tiger reserves in the satpura-maikal landscape of central india. we used noninvasively collected fecal and hair samples to obtain genotypic ...201525945939
on the identity of the tiger beetle cicindela philippinensis mandl, 1956 (coleoptera: carabidae: cicindelinae).in the course of preparing a key to the philippine tiger beetle genera (pangantihon & zettel, in prep.) it became necessary to examine the holotype of cicindela philippinensis mandl, 1956, the only specimen from the philippines that has been tentatively assigned to the genus myriochila motschulsky, 1857.201525947531
a new species micrarctia kautti (lepidoptera: erebidae, arctiinae) from west china.a new tiger moth, micrarctia kautti sp. n., from southwest china, sichuan is described. a diagnostic comparison with micrarctia trigona (leech, 1899) is provided. during recent years the chinese arctiini have been intensively collected and explored (dubatolov, 1996a; dubatolov, 1996b; dubatolov, 2003; dubatolov, kishida & wu, 2005; fang & cao, 1984; fang, 2000 and numerous other publications) so the discovery of a striking new species by sergey murzin in the dafengding mountains, sichuan provinc ...201525947852
the development and application of a multiplex short tandem repeat (str) system for identifying subspecies, individuals and sex in tigers.poaching and trans-boundary trafficking of tigers and body parts are threatening the world's last remaining wild tigers. development of an efficient molecular genetic assay for tracing the origins of confiscated specimens will assist in law enforcement and wildlife forensics for this iconic flagship species. we developed a multiplex genotyping system "tigrisplex" to simultaneously assess 22 short tandem repeat (str, or microsatellite) loci and a gender-identifying sry gene, all amplified in 4 re ...201525950598
telling the tiger by its stripes: mapping the genomics of kidney graft tolerance in real time.though the majority of kidney allografts are eventually lost to the process of chronic rejection, there are instances when kidney function is maintained after patients have stopped their immunosuppression. baron and colleagues have examined the blood gene signature of patients with spontaneous kidney tolerance and identified a series of genes that they suggest define kidney graft acceptance. this exciting development provides a potential list of biomarkers defining immunological tolerance in hum ...201525951065
curtain eruptions from enceladus' south-polar terrain.observations of the south pole of the saturnian moon enceladus revealed large rifts in the south-polar terrain, informally called 'tiger stripes', named alexandria, baghdad, cairo and damascus sulci. these fractures have been shown to be the sources of the observed jets of water vapour and icy particles and to exhibit higher temperatures than the surrounding terrain. subsequent observations have focused on obtaining close-up imaging of this region to better characterize these emissions. recent w ...201525951283
defining the eye-of-the-tiger sign. 201525961178
defining the eye-of-the-tiger sign-reply. 201525961179
mammalian gastrointestinal parasites in rainforest remnants of anamalai hills, western ghats, india.habitat fragmentation is postulated to be a major factor influencing infectious disease dynamics in wildlife populations and may also be responsible, at least in part, for the recent spurt in the emergence, or re-emergence, of infectious diseases in humans. the mechanism behind these relationships are poorly understood due to the lack of insights into the interacting local factors and insufficient baseline data in ecological parasitology of wildlife. here, we studied the gastrointestinal parasit ...201525963266
identification and characterisation of putative seminal fluid proteins from male reproductive tissue est libraries in tiger beetles.the study of proteins transferred through semen can provide important information for biological questions such as adaptive evolution, the origin of new species and species richness. the objective of this study was to identify seminal fluid proteins (sfps) that may contribute to the study of the reproductive system of tiger beetles (cicindelids), a group of more than 2,500 species distributed worldwide that occupy a great diversity of habitats.201525981911
transcriptome profiling and genetic study reveal amplified carboxylesterase genes implicated in temephos resistance, in the asian tiger mosquito aedes albopictus.the control of aedes albopictus, a major vector for viral diseases, such as dengue fever and chikungunya, has been largely reliant on the use of the larvicide temephos for many decades. this insecticide remains a primary control tool for several countries and it is a potential reliable reserve, for emergency epidemics or new invasion cases, in regions such as europe which have banned its use. resistance to temephos has been detected in some regions, but the mechanism responsible for the trait ha ...201526000638
coalescence vs. concatenation: sophisticated analyses vs. first principles applied to rooting the angiosperms.it has recently been concluded that phylogenomic data from 310 nuclear genes support the clade of (amborellales, nymphaeales) as sister to the remaining angiosperms and that shortcut coalescent phylogenetic methods outperformed concatenation for these data. we falsify both of those conclusions here by demonstrating that discrepant results between the coalescent and concatenation analyses are primarily caused by the coalescent methods applied (mp-est and star) not being robust to the highly diver ...201526002829
stability and change in adjustment profiles among chinese american adolescents: the role of parenting.asian american adolescents are often depicted as academically successful but psychologically distressed, a pattern known as the achievement/adjustment paradox. in a sample of 444 chinese american adolescents (54 % females), we identified three distinct patterns of adjustment in early adolescence, middle adolescence, and emerging adulthood: the well-adjusted group, which was the largest, exhibited high achievement and low psychological distress; the poorly-adjusted group exhibited poor achievemen ...201526022414
some like it hot: thermal tolerance and oxygen supply capacity in two eurythermal crustaceans.thermal sensitivity of the cardiorespiratory oxygen supply capacity has been proposed as the cardinal link underlying the upper boundary of the temperature niche in aquatic ectotherms. here we examined the evidence for this link in two eurythermal decapods, the giant tiger shrimp (penaeus monodon) and the european crayfish (astacus astacus). we found that both species have a temperature resistant cardiorespiratory system, capable of maintaining oxygen delivery up to their upper critical temperat ...201526030412
social structure and space use of amur tigers (panthera tigris altaica) in southern russian far east based on gps telemetry data.to better understand the spatial structure of amur tigers (panthera tigris altaica) at the southern edge of their range we fitted 14 tigers (6♀♀ and 8♂♂) with 15 gps-argos collars between 2008 and 2011 in 2 study sites: the ussuriskii reserve of southern sikhote-alin and the land of the leopard national park in southwest primorye, russian far east. fixed kernel estimates of male home ranges were larger than those of female home ranges (p < 0.05 [mean 95% fixed kernel(♀) = 401 ± 205 km(2) ; mean ...201526037451
population dynamics of amur tigers (panthera tigris altaica) in sikhote-alin biosphere zapovednik: 1966-2012.in 2010, the world's tiger (panthera tigris) range countries agreed to the goal of doubling tiger numbers over 12 years, but whether such an increase is biologically feasible has not been assessed. long-term monitoring of tigers in sikhote-alin biosphere zapovednik (sabz), russia provided an opportunity to determine growth rates of a recovering population. a 41-year growth phase was followed by a rapid decline in tiger numbers. annual growth rates during the growth phase averaged 4.6%, beginning ...201526037543
responses of tadpoles to hybrid predator odours: strong maternal signatures and the potential risk/response mismatch.previous studies have established that when a prey animal knows the identity of a particular predator, it can use this knowledge to make an 'educated guess' about similar novel predators. such generalization of predator recognition may be particularly beneficial when prey are exposed to introduced and invasive species of predators or hybrids. here, we examined generalization of predator recognition for woodfrog tadpoles exposed to novel trout predators. tadpoles conditioned to recognize tiger tr ...026041358
discrimination factors of carbon and nitrogen stable isotopes from diet to hair and scat in captive tigers (panthera tigris) and snow leopards (uncia uncia).in order to use stable isotope ratio values obtained from wild animal tissues, we must accurately calculate the differences in isotope ratios between diet and consumer (δtissue - δdiet). these values, called trophic discrimination factors (tdfs, denoted with ∆), are necessary for stable isotope ecology studies and are best calculated in controlled environments.201526044274
fatal tiger shark, galeocerdo cuvier attack in new caledonia erroneously ascribed to great white shark, carcharodon carcharias.to understand the causes and patterns of shark attacks on humans, accurate identification of the shark species involved is necessary. often, the only reliable evidence for this comes from the characteristics of the wounds exhibited by the victim. the present case report is intended as a reappraisal of the luengoni, 2007 case (international shark attack file no. 4299) where a single shark bite provoked the death of a swimmer by haemorrhagic shock. our examination of the wounds on the body of the ...201526048500
repeated, long-distance migrations by a philopatric predator targeting highly contrasting ecosystems.long-distance movements of animals are an important driver of population spatial dynamics and determine the extent of overlap with area-focused human activities, such as fishing. despite global concerns of declining shark populations, a major limitation in assessments of population trends or spatial management options is the lack of information on their long-term migratory behaviour. for a large marine predator, the tiger shark galeocerdo cuvier, we show from individuals satellite-tracked for mu ...201526057337
glucocorticoid stress responses of reintroduced tigers in relation to anthropogenic disturbance in sariska tiger reserve in india.tiger (panthera tigris), an endangered species, is under severe threat from poaching, habitat loss, prey depletion and habitat disturbance. such factors have been reported causing local extermination of tiger populations including in one of the most important reserves in india, namely sariska tiger reserve (str) in northwestern india. consequently, tigers were reintroduced in str between 2008 and 2010, but inadequate breeding success was observed over the years, thus invoking an investigation to ...201526061171
autochthonous chikungunya transmission and extreme climate events in southern france.extreme precipitation events are increasing as a result of ongoing global warming, but controversy surrounds the relationship between flooding and mosquito-borne diseases. a common view among the scientific community and public health officers is that heavy rainfalls have a flushing effect on breeding sites, which negatively affects vector populations, thereby diminishing disease transmission. during 2014 in montpellier, france, there were at least 11 autochthonous cases of chikungunya caused by ...201526079620
quantitative methods of identifying the key nodes in the illegal wildlife trade network.innovative approaches are needed to combat the illegal trade in wildlife. here, we used network analysis and a new database, healthmap wildlife trade, to identify the key nodes (countries) that support the illegal wildlife trade. we identified key exporters and importers from the number of shipments a country sent and received and from the number of connections a country had to other countries over a given time period. we used flow betweenness centrality measurements to identify key intermediary ...201526080413
yeast surface display of two proteins previously shown to be protective against white spot syndrome virus (wssv) in shrimp.cell surface display using the yeasts saccharomyces cerevisiae and pichia pastoris has been extensively developed for application in bioindustrial processes. due to the rigid structure of their cell walls, a number of proteins have been successfully displayed on their cell surfaces. it was previously reported that the viral binding protein rab7 from the giant tiger shrimp penaeus monodon (pmrab7) and its binding partner envelope protein vp28 of white spot syndrome virus (wssv) could independentl ...201526083446
conservation conflict: factor people into tiger conservation. 201526085259
pathology in captive wild felids at german zoological gardens.this retrospective study provides an overview on spontaneous diseases occurring in 38 captive wild felids submitted for necropsy by german zoological gardens between 2004 and 2013. species included 18 tigers, 8 leopards, 7 lions, 3 cheetahs and 2 cougars with an age ranging from 0.5 to 22 years. renal lesions, predominantly tubular alterations (intra-tubular concrements, tubular degeneration, necrosis, intra-tubular cellular debris, proteinaceous casts, dilated tubuli) followed by interstitial ( ...201526086731
seasonal acclimatization determined by non-invasive measurements of coat insulation.seasonal acclimatization in terrestrial mammals in the northern hemisphere involves changes in coat insulation. it is more economical to provide increased insulation than increased heat production for protection against the cold. this study was done to test a technique for the non-invasive measurement of mammal coat insulation and to measure coat insulation over several seasons on captive exotics. the working hypothesis was that species that have no coat or have a coat that does not change seaso ...201526087320
the insect growth regulator pyriproxyfen terminates egg diapause in the asian tiger mosquito, aedes albopictus.the asian tiger mosquito, aedes albopictus, is a highly invasive mosquito species that transmits chikungunya and dengue. this species overwinters as diapausing eggs in temperate climates. early diapause termination may be a beneficial strategy for winter mosquito control; however, a mechanism to terminate the diapause process using chemicals is not known. we tested the hypothesis that a hormonal imbalance caused by the administration of juvenile hormone analog would terminate egg diapause in a. ...201526090954
the amur tiger in northeast asia: conservation and ecology of an endangered subspecies. 201526096297
amur tiger conservation education program: a pilot study on program effectiveness.anthropogenic impacts are the primary threats to amur tigers (panthera tigris altaica) and their habitat. villagers living in proximity to tigers tend to view them negatively and, often, as a source of revenue on black markets. we aim to reduce human-tiger conflict by working with young students of ternei county in the heart of tiger habitat in primorskii krai (province). to inform and influence ternei county's future decision-makers, we developed "safe conduct", a year-long education program he ...201526096458
identifying ecological corridors for amur tigers (panthera tigris altaica) and amur leopards (panthera pardus orientalis).the rapid explosion of human populations and the associated development of human-dominated landscapes have drastically reduced and fragmented habitat for tigers (panthera tigris) and leopards (panthera pardus) across asia, resulting in multiple small populations. however, amur tiger (panthera tigris altaica) habitat in russia has remained largely interconnected, except for a break between tigers in southwest primorye and the southern sikhote-alin mountains. this habitat patch in southwest primor ...201526096589
mortality of amur tigers: the more things change, the more they stay the same.poaching as well as loss of habitat and prey are identified as causes of tiger population declines. although some studies have examined habitat requirements and prey availability, few studies have quantified cause-specific mortality of tigers. we used cumulative incidence functions (cifs) to quantify cause-specific mortality rates of tigers, expanding and refining earlier studies to assess the potential impact of a newly emerging disease. to quantify changes in tiger mortality over time, we re-e ...201526096683
cytauxzoonosis in cats: abcd guidelines on prevention and management.cytauxzoon species are apicomplexan haemoparasites, which may cause severe disease in domestic cats, as well as lions and tigers. for many years, cytauxzoonosis in domestic cats was only reported in north and south america, but in recent years the infection has also been seen in europe (spain, france and italy).201526101317
correction: tigers of sundarbans in india: is the population a separate conservation unit? 201526106892
colour polymorphism torn apart by opposing positive frequency-dependent selection, yet maintained in space.polymorphic warning signals in aposematic species are enigmatic because predator learning and discrimination should select for the most common coloration, resulting in positive frequency-dependent survival selection. here, we investigated whether differential mating success could create sufficiently strong negative frequency-dependent selection for rare morphs to explain polymorphic (white and yellow) warning coloration in male wood tiger moths (parasemia plantaginis). we conducted an experiment ...201526114930
two fatal tiger attacks in zoos.two captive tiger attacks are presented that took place in cologne and münster zoos. both attacks occurred when the handlers, intent on cleaning the enclosures, entered whilst the tigers accidently retained access to the location, and thus defended their territory against the perceived intruders. both victims suffered fatal neck injuries from the bites. at münster, colleagues managed to lure the tiger away from its victim to enable treatment, whilst the cologne zoo tiger had to be shot in order ...201626115644
oxidative stress response of the black tiger shrimp penaeus monodon to vibrio parahaemolyticus challenge.vibrio parahaemolyticus is a virulent pathogen that affects shrimp aquaculture. reactive oxygen species are produced by the immune system that defends the host against foreign microorganisms. in the present study, the oxidative stress response in hepatopancreas and gills of penaeus monodon to v. parahaemolyticus challenge were studied, such as respiratory burst, ros production (·o2(-) and ·oh), activities of antioxidant enzymes (cat, gpx, sod, pod and gst) and oxidative damage to lipid and prote ...201526142143
conceptual framework and quantification of population vulnerability for effective emergency response planning.the study focused on the methodological advancement and analytical approach of using multilevel data to define population vulnerability and risk in bioemergency disaster planning.201526150366
machine learning approach for pooled dna sample calibration.despite ongoing reduction in genotyping costs, genomic studies involving large numbers of species with low economic value (such as black tiger prawns) remain cost prohibitive. in this scenario dna pooling is an attractive option to reduce genotyping costs. however, genotyping of pooled samples comprising dna from many individuals is challenging due to the presence of errors that exceed the allele frequency quantisation size and therefore cannot be simply corrected by clustering techniques. the s ...201526156142
male enhancement nutraceuticals in the middle east market: claim, pharmaceutical quality and safety assessments.the global market is invaded by male enhancement nutraceuticals claimed to be of natural origin sold with a major therapeutic claim. most of these products have been reported by international systems like the food and drug administration (fda). we hypothesize that these products could represent a major threat to the health of the consumers. in this paper, pharmaceutical evaluation of some of these nutraceutical products sold in egypt under the therapeutic claim of treating erectile dysfunction, ...201526165612
larvicidal spirobisnaphthalenes from the endophytic fungus berkleasmium sp. against aedes albopictus.in our screening programme for new agrochemicals from endophytic fungi, the ethyl acetate extract of an endophytic berkleasmium sp. isolated from the medicinal plant dioscorea zingiberensis was found to possess strong larvicidal activity against the asian tiger mosquito, aedes albopictus.201626171851
traditional medicines: tiger-bone trade could threaten lions. 201526178954
established population of the invasive mosquito species aedes albopictus in romania, 2012-14.during an entomological investigation carried out in bucharest and surroundings in fall of 2012, 45 adult mosquitoes (38 females and 7 males) of aedes albopictus were collected in a neighborhood from the southern area of the city. the morphological identification of the species was further confirmed by sequencing 2 mitochondrial dna markers: the cytochrome c oxidase subunit i and nadh dehydrogenase subunit 5 genes. aedes albopictus was collected again in 2013 in the same area from july until oct ...201526181695
epigenetic alterations and decreasing insecticide sensitivity of the asian tiger mosquito aedes albopictus.a range of environmental factors, including chemicals, can affect epigenetic processes in organisms leading to variations in phenotype. thus, epigenetics displays an important environmentally responsive element. the transgenerational impact of environmental stressors on dna methylation and phenotype was the focus of this study. the influence of two known dna methylation-changing agents, the phytoestrogen genistein and the fungicide vinclozolin, on the overall dna methylation level in the asian t ...201526188644
non-alcoholic fatty liver disease-related hepatocellular carcinoma: a sleeping tiger in the asia pacific.the epidemiology of hepatocellular carcinoma (hcc) in the asia pacific will undergo significant change over the next few decades as the prevalence of viral hepatitis declines and the burden of metabolic diseases increases. as the asia pacific embraces continued affluence, obesity and diabetes rates are burgeoning, becoming increasingly important to the incidence of hcc. obesity and diabetes are established risk factors for hcc, either as substrates for non-alcoholic fatty liver disease (nafld) o ...201326202297
first report of the invasive mosquito species aedes koreicus in the swiss-italian border region.in 2012 and 2013, an entomological survey of aedes albopictus, the asian tiger mosquito, was carried out in the border region of southern switzerland and northern italy, using ovitraps. in july 2013, besides a. albopictus already known to the region several unusual eggs were recovered.201526223377
gnathostoma spinigerum mitochondrial genome sequence: a novel gene arrangement and its phylogenetic position within the class chromadorea.human gnathostomiasis is an emerging food-borne parasitic disease caused by nematodes in the genus gnathostoma. in spite of their significance as pathogens, these parasites remain poorly understood at the molecular level. in the present study, we sequenced the mitochondrial (mt) genome of g. spinigerum, which infects a range of definitive hosts including dogs, cats, tigers, leopards and humans. the mt genome of g. spinigerum is 14,079 bp in size and shows substantial changes in gene order compar ...201526228511
phytochemical constituents from the florets of tiger grass thysanolaena latifolia from nepal.phytochemical investigation on the florets of thysanolaena latifolia leads to the isolation of a new compound 6″-o-acetylorientin-2″-o-α-l-rhamnopyranoside (1), named amrisoside and other 34 known compounds. the chemical structures of the compounds were determined from the interpretation of spectroscopic data including nmr, ms, and ir. this is the first report of phytochemical constituents from the monotypic genus thysanolaena.201626230153
forensic science in support of wildlife conservation efforts - developments in morphological and chemical approaches in taiwan.parts from animals that are now endangered species (such as bear bile, rhino horn, and tiger bone) have long been important ingredients of traditional chinese medicine (tcm). deeply believing in the potency of these ingredients and coupled with substantial gains in wealth of the population in taiwan in the 1980s, ugly scenarios came to light. taiwan quickly became the target of investigation pursued by international wildlife conservationists. to provide scientific bases for the government's inve ...201126231168
sound production in the tiger-tail seahorse hippocampus comes: insights into the sound producing mechanisms.acoustic signals of the tiger-tail seahorse (hippocampus comes) during feeding were studied using wavelet transform analysis. the seahorse "click" appears to be a compounded sound, comprising three acoustic components that likely come from two sound producing mechanisms. the click sound begins with a low-frequency precursor signal, followed by a sudden high-frequency spike that decays quickly, and a final, low-frequency sinusoidal component. the first two components can, respectively, be traced ...201526233039
efficacy of two common methods of application of residual insecticide for controlling the asian tiger mosquito, aedes albopictus (skuse), in urban areas.after its first introduction in the 1980's the asian tiger mosquito, aedes albopictus (skuse), has spread throughout southern europe. ae. albopictus is considered an epidemiologically important vector for the transmission of many viral pathogens such as the yellow fever virus, dengue fever and chikungunya fever, as well as several filarial nematodes such as dirofilaria immitis or d. repens. it is therefore crucial to develop measures to reduce the risks of disease transmission by controlling the ...201526248028
age and growth of the tiger shark galeocerdo cuvier off the east coast of australia.total lengths (l(t)) at age and growth rates for south-west pacific galeocerdo cuvier were estimated from vertebral growth-band counts of 202 sagitally sectioned centra from 112 females (71-430 cm l(t)), 79 males (72-351 cm l(t)) and 11 of unknown sex. captive growth data were also examined to complement vertebral age estimations. the sexes combined modelled growth coefficient (k = 0.08) was smaller than previously reported for g. cuvier populations elsewhere. split-band and narrow banding patte ...201526248806
effects of developmental temperature on gametocysts and oocysts of two species of gregarines blabericola migrator and blabericola cubensis (apicomplexa: eugregarinida: blabericolidae) parasitizing blaberid cockroaches (dictyoptera: blaberidae).abiotic environmental conditions, especially temperature and humidity, have profound effects on the growth and development of gregarines, but these effects remain largely undocumented. quantifying the effects of environmental conditions on the growth and development of exogenous gregarine ontogenetic stages is an important first step in understanding the transmission, population dynamics, and environmental persistence of gregarine infection. in this study, we examined the effect of 6 environment ...201526248890
magnetic resonance imaging study in a normal bengal tiger (panthera tigris) stifle joint.the purpose of this study was to describe the normal appearance of the bony and soft tissue structures of the stifle joint of a bengal tiger (panthera tigris) by low-field magnetic resonance imaging (mri), and the use of gross anatomical dissections performed as anatomical reference. a cadaver of a mature female was imaged by mri using specific sequences as the spin-echo (se) t1-weighting and gradient-echo (ge) stir t2-weighting sequences in sagittal, dorsal and transverse planes, with a magnet ...201526260666
informatics competencies in nursing management.this study aimed to identify in scientific literature the informatics competencies required from the nurses to make decision in management process. through a scoping review, literature databases were searched to find articles published in portuguese, english, or spanish, until july 2013. 188 articles were found, and seven were included in this study, published between 1994 and 2011. the studies were written in english (5; 71%), in usa (5; 71%), using experience reports or literature review desig ...201526262314
a shrimp pacifastin light chain-like inhibitor: molecular identification and role in the control of the prophenoloxidase system.pacifastin is a recently classified family of serine proteinase inhibitors that play essential roles in various biological processes, including in the regulation of the melanization cascade. here, a novel pacifastin-related gene, termed pmpacifastin-like, was identified from a reverse suppression subtractive hybridization (ssh) cdna library created from hemocytes of the prophenoloxidase pmpropo1/2 co-silenced black tiger shrimp penaeus monodon. the full-length sequences of pmpacifastin-like and ...201626271600
pseudorhabdosynochus species (monogenoidea, diplectanidae) parasitizing groupers (serranidae, epinephelinae, epinephelini) in the western atlantic ocean and adjacent waters, with descriptions of 13 new species.seventeen of twenty-three species of groupers collected from the western atlantic ocean and adjacent waters were infected with 19 identified species (13 new) of pseudorhabdosynochus yamaguti, 1958 (dactylogyridea, diplectanidae); specimens of the spanish flag gonioplectrus hispanus, coney cephalopholis fulva, marbled grouper dermatolepis inermis, mutton hamlet alphestes afer, and misty grouper hyporthodus mystacinus were not infected; the yellowmouth grouper mycteroperca interstitialis and yello ...201526272242
survey of voice acoustic parameters in iranian female teachers.teachers are one of the professional voice users. voice problems are common among them. female teachers are known to have more voice problems than male ones. furthermore, there are only few studies on the voice of iranian female teachers. the present study investigated the acoustic parameters of voice in iranian female teachers and compares them with nonteachers.201626275636
new yellow head virus genotype (yhv7) in giant tiger shrimp penaeus monodon indigenous to northern australia.in 2012, giant tiger shrimp penaeus monodon originally sourced from joseph bonaparte gulf in northern australia were examined in an attempt to identify the cause of elevated mortalities among broodstock at a queensland hatchery. nucleic acid extracted from ethanol-fixed gills of 3 individual shrimp tested positive using the oie yhv protocol 2 rt-pcr designed to differentiate yellow head virus (yhv1) from gill-associated virus (gav, synonymous with yhv2) and the oie yhv protocol 3 rt-nested pcr d ...201526290511
effects of various fibre-rich extracts on cholesterol binding capacity during in vitro digestion of pork patties.intake of foods containing high levels of cholesterol harms human health, and an increase in the intake of dietary fibre (df) may mitigate these negative effects. the co-products obtained from fruit juice extraction (lemon, grapefruit and pomegranate), lemon ice-cream production and tiger nut "horchata" (beverage) have been used for the production of fibre-rich extracts used as dietary fibre sources. the purpose of this study was to examine the effect of these fibre source additions on cholester ...201526292939
antimicrobial activities of actinomycetes isolated from unexplored regions of sundarbans mangrove ecosystem.new broad spectrum antimicrobial agents are urgently needed to combat frequently emerging multi drug resistant pathogens. actinomycetes, the most talented group of microorganisms isolated from unexplored regions of the world may be the ultimate solution to this problem. thus the aim of this study was to isolate several bioactive actinomycetes strains capable of producing antimicrobial secondary metabolite from sundarbans, the only mangrove tiger land of the world.201526293487
costs and benefits of plant allelochemicals in herbivore diet in a multi enemy world.sequestration of plant defensive chemicals by herbivorous insects is a way of defending themselves against their natural enemies. such herbivores have repeatedly evolved bright colours to advertise their unpalatability to predators, i.e. they are aposematic. this often comes with a cost. in this study, we examined the costs and benefits of sequestration of iridoid glycosides (igs) by the generalist aposematic herbivore, the wood tiger moth, parasemia plantaginis. we also asked whether the defenc ...201526296333
determination of tocopherols and sitosterols in seeds and nuts by quechers-liquid chromatography.in the present work a simple, reliable and affordable sample treatment method for the simultaneous analysis of tocopherols and free phytosterols in nuts was developed. analyte extraction was carried out using the quechers methodology and analyte separation and detection were accomplished using hplc-dad. the use of this methodology for the extraction of natural occurring substances provides advantages such as speed, simplicity and ease of use. the parameters evaluated for the validation of the me ...201626304416
presumed secondary immune-mediated haemolytic anaemia following elapid snake envenomation and its treatment in four dogs.this case series describes secondary immune-mediated haemolytic anaemia (imha) in four dogs following elapid snake envenomation and its treatment. all the dogs initially presented with clinical signs commensurate with mainland tiger snake (notechis scutatus) envenomation. none of the dogs was anaemic at the time of presentation. imha was diagnosed 3-9 days following snake envenomation. the trigger for imha was unclear in each case and may have been a component of the snake venom, antivenom, fres ...201526313210
shotgun proteomic analysis of tiger milk mushroom (lignosus rhinocerotis) and the isolation of a cytotoxic fungal serine protease from its sclerotium.the sclerotium of lignosus rhinocerotis (cooke) ryvarden (tiger milk mushroom) has been traditionally used as a complementary and alternative medicine for cancer treatment by the local communities of southeast asia. despite the continuous research interest in its antiproliferative activity, the identity of the bioactive compound(s) responsible has yet to be determined. this study aims to bridge the gap in existing research literature by using proteomics approach for investigation of the nature o ...201526320692
correction to 'present and future projections of habitat suitability of the asian tiger mosquito, a vector of viral pathogens, from global climate simulation'. 026323769
characterization of the complete mitochondrial genome of the scarlet tiger moth callimorpha dominula (insecta: lepidoptera: arctiidae).the complete mitochondrial genome of the scarlet tiger moth callimorpha dominula (insecta: lepidoptera: arctiidae) has been reconstructed from the whole-genome illumina sequencing data. this circular genome is 15 496 bp in size, and contains 13 protein-coding genes (pcgs), 22 transfer rnas (trnas), two ribosomal rnas (rrnas), and one a + t-rich d-loop or control region. most pcgs are initiated with the atn codons, except for cox1 with the unusual cga as its initiation codon. four pcgs (cox1, cox ...201626329289
tiger, bengal and domestic cat embryos produced by homospecific and interspecific zona-free nuclear transfer.the aim of this study was to evaluate three different cloning strategies in the domestic cat (felis silvestris) and to use the most efficient to generate wild felid embryos by interspecific cloning (iscnt) using bengal (a hybrid formed by the cross of felis silvestris and prionailurus bengalensis) and tiger (panthera tigris) donor cells. in experiment 1, zona-free (zp-free) cloning resulted in higher fusion and expanded blastocyst rates with respect to zona included cloning techniques that invol ...201526332056
high resolution spatial analysis of habitat preference of aedes albopictus (diptera: culicidae) in an urban environment.over the past decades, the asian tiger mosquito (aedes albopictus (skuse, 1895)) has emerged in many countries, and it has colonized new environments, including urban areas. the species is a nuisance and a potential vector of several human pathogens, and a better understanding of the habitat preferences of the species is needed for help in successful prevention and control. so far, the habitat preference in urban environments has not been studied in southern european cities. in this paper, spati ...201526334806
density-dependent oviposition by female aedes albopictus (diptera: culicidae) spreads eggs among containers during the summer but accumulates them in the fall.when possible, oviparous females should deposit eggs in sites that maximize the future performance of their offspring. therefore, studies of oviposition behavior may uncover parameters important to offspring fitness. gravid female aedes albopictus (skuse) were given a choice of containers with leaf infusion or plain water, either open or with a cover with a small opening, and their behavior was compared under summer (long day, higher temperature) or fall (short day, lower temperature) regimes. o ...201526335478
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