Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| occurrence and prevalence of selected zoonotic agents: echinococcus multilocularis, trichinella spiralis and hepatitis e virus (hev) in the population of polish hunters--results of the study conducted in 2010-2012. | in poland the development of the knowledge concerning zoonotic pathogens, of which free-living animals are the reservoir of is gaining in importance both in epidemiological aspect as well as in the context of prevention for improving public health. dietary habits such as the consumption of forest undergrowth products and wild game meat, and the way of those products being prepared (in the process of barbequing) pose a risk factors of infection with the foodborne pathogens such as echinococcus mu ... | 2015 | 27139343 |
| activation and recruitment of regulatory t cells via chemokine receptor activation in trichinella spiralis-infected mice. | as most infections by the helminth parasite elicit the recruitment of cd4(+)cd25(+)foxp3(+) t (treg) cells, many scientists have suggested that these cells could be used for the treatment of immune-mediated inflammation and associated diseases. in order to investigate the distribution and alteration of activated treg cells, we compared the expression levels of treg cell activation markers in the ileum and gastrocnemius tissues 1, 2, and 4 weeks after infection. the number of treg cells was monit ... | 2016 | 27180574 |
| helminth parasites in black rats (rattus rattus) and brown rats (rattus norvegicus) from different environments in the netherlands. | rattus norvegicus (brown rat) and rattus rattus (black rat) are known carriers of bacteria, viruses, and parasites of zoonotic and veterinary importance. moreover, rats may play a role in the transmission of muscle larvae of the zoonotic nematode trichinella spiralis to farm animals. we aimed to study the intestinal and intramuscular helminths in wild rats from three different environments to assess the relevance of rats as carrier of zoonotic parasites for public health. | 2016 | 27193418 |
| regulatory parameters of the lung immune response during the early phase of experimental trichinellosis. | parasitic infection caused by trichinella spiralis provokes an early stimulation of the mucosal immune system which causes an allergic inflammatory response in the lungs. the present work was intended to characterize the kinetics of emergence of regulatory parameters in wistar rat lungs during this early inflammatory response, between days 0 and 13p.i. the presence of regulatory cells such as regulatory t cells (tregs) and alternatively activated macrophages (aam) was analyzed in lung cell suspe ... | 2016 | 27211081 |
| trichinella spiralis in road-killed raccoon dogs (nyctereutes procyonoides) in western poland. | trichinellosis is still one of the most important food-borne parasitic zoonoses and is considered as a threat to public health worldwide. the aim of this study was to use genotyping techniques to determine the prevalence of trichinella species in wild raccoon dogs (nyctereutes procyonoides) in western poland. the infection rate in raccoon dogs was 0.8%. all infections were due to t. spiralis. | 2016 | 27262962 |
| serglycin proteoglycans limit enteropathy in trichinella spiralis-infected mice. | serglycin proteoglycans are essential for maturation of secretory granules and for the correct granular storage of cationic proteases in hematopoietic cells, e.g. mast cells. however, little is known about the in vivo functions of serglycin proteoglycans during infection. here we investigated the potential role of serglycin proteoglycans in host defense after infection with the nematode trichinella spiralis. | 2016 | 27267469 |
| cloning and characterization of thioredoxin peroxidases from trichinella spiralis. | the intracellular parasitic nematode, trichinella spiralis, can initiate a high level of oxidative stress, especially during rapid growth and generative propagation phases. thioredoxin peroxidases (tpxs) protect helminths against oxidative stress, but none has been identified in t. spiralis. here, 3 members of the tpx family were cloned from t. spiralis muscle larvae (ml). the lengths of tstpx orfs were 747bp, 588bp and 594bp, respectively, and the deduced proteins predicted to contain ahpc-tsa ... | 2016 | 27283449 |
| recombinant trichinella spiralis 53-kda protein activates m2 macrophages and attenuates the lps-induced damage of endotoxemia. | sepsis is a serious clinical condition of excessive systemic immune response to microbial infection. the pro-inflammatory stage of sepsis is generally launched by innate cells such as macrophages. they release inflammatory cytokines, activate other immune cells and cause severe tissue/organ damage. in this study, we have revealed that recombinant trichinella spiralis (ts) excretory-secretory protein (rtsp53) exhibited anti-inflammatory properties and rescued mice from lps-induced endotoxemia, wh ... | 2016 | 27312706 |
| trichinella spiralis infection and transplacental passage in human pregnancy. | infection by trichinella spp. during pregnancy still arises many questions. worldwide an important number of trichinellosis outbreaks have occurred over time where pregnant women were or might have been involved. parasitological and immunoserological parameters, clinical background and transplacental passage of trichinella spiralis of six pregnant women, from different outbreaks, were studied. serum samples were collected from patients and their children: at pregnancy, at delivery, newborn and/o ... | 2016 | 27349972 |
| identification and characterization of cd4(+) t cell epitopes present in trichinella spiralis paramyosin. | trichinellosis is a worldwide zoonosis and vaccinating swine with a potent vaccine is a practical approach to prevent trichinella infections in china. paramyosin of t. spiralis (ts-pmy) was shown in our previous work to be a good vaccine candidate against trichinella infections. because cd4(+) t cells play a crucial role in effective immunity against t. spiralis infection, identifying cd4(+) t cell epitopes of paramyosin is crucial for constructing a chimeric subunit epitope vaccine. twelve cd4( ... | 2016 | 27353726 |
| proteomic analysis of differentially expressed proteins in the three developmental stages of trichinella spiralis. | trichinella spiralis, an intracellular parasitic nematode, can cause severe foodborne zoonosis, trichinellosis. the life cycle of t. spiralis consists of adult (ad), muscle larvae (ml) and newborn larvae (nbl). the protein profiles in different developmental stages of the parasite remain unknown. in the present study, proteins from lysates of ad, ml and nbl were identified by isobaric tags for relative and absolute quantitation (itraq). a total of 4691 proteins were identified in all the develop ... | 2016 | 27357750 |
| evaluation of protective efficacy induced by virus-like particles containing a trichinella spiralis excretory-secretory (es) protein in mice. | the frequent outbreaks of human trichinellosis globally underscore the need to develop effective vaccine. we hypothesized that a novel vaccine could improve vaccine efficacy against trichinella spiralis. | 2016 | 27378450 |
| partially protective immunity induced by the 14-3-3 protein from trichinella spiralis. | trichinellosis, a widespread zoonosis, is considered to be an emerging or re-emerging infectious parasitic disease. the development of vaccines to prevent trichinella infection in domestic animals and humans is important for disease control. in a previous study, we identified ts14-3-3 as an immunodominant protein from trichinella spiralis (t. spiralis) adult worms recognized by early infection sera from pigs and mice. in this study, we further confirmed that ts14-3-3 mrna is expressed in both ad ... | 2016 | 27387374 |
| proteomic analysis and immunodetection of antigens from early developmental stages of trichinella spiralis. | trichinella spiralis is an ubiquitous parasitic nematode that lives in muscle tissue of many hosts and causes trichinellosis in humans. numerous efforts have been directed at specific detection of this infection and strategies for its control. tsl-1 and other antigens, mainly from muscle larvae (ml), have been used to induce partial protection in rodents. an improvement in protective immunity may be achieved by using antigens from other parasite stages. further, identification of other parasite ... | 2016 | 27396501 |
| analyses of compact trichinella kinomes reveal a mos-like protein kinase with a unique n-terminal domain. | parasitic worms of the genus trichinella (phylum nematoda; class enoplea) represent a complex of at least twelve taxa that infect a range of different host animals, including humans, around the world. they are foodborne, intracellular nematodes, and their life cycles differ substantially from those of other nematodes. the recent characterization of the genomes and transcriptomes of all twelve recognized taxa of trichinella now allows, for the first time, detailed studies of their molecular biolo ... | 2016 | 27412987 |
| effect of probiotic bacteria on phagocytosis and respiratory burst activity of blood polymorphonuclear leukocytes (pmnl) in mice infected with trichinella spiralis. | this study focusses on the effect of probiotic (bacteriocinogenic) strains on parasite infection and innate immunity - phagocytosis and oxidative burst of blood monocytes and polymorphonuclear leukocytes (pmnl) in mice infected with trichinella spiralis. bacteriocinogenic and probiotic strains of different origin (enterococcus faecium al41=ccm8558, enterococcus durans ed26e/7, lactobacillus fermentum ad1=ccm7421, lactobacillus plantarum 17l/1) were administered daily in dose of 10(9)cfu/ml in 10 ... | 2016 | 27425573 |
| trichinella spiralis: adaptation and parasitism. | publication of the genome from the clade i organism, trichinella spiralis, has provided us an avenue to address more holistic problems in parasitology; namely the processes of adaptation and the evolution of parasitism. parasitism among nematodes has evolved in multiple, independent events. deciphering processes that drive species diversity and adaptation are keys to understanding parasitism and advancing control strategies. studies have been put forth on morphological and physiological aspects ... | 2016 | 27425574 |
| validation studies of the latex agglutination test for the detection of trichinella larvae in meat products. | human trichinellosis is a foodborne disease caused by ingestion of meat infected with trichinella muscle larvae. to control trichinella spp. infection in the european union, all slaughtered pigs from holdings that are not officially recognized as applying controlled housing conditions and other animals susceptible to trichinella infection and intended for human consumption should be examined by one of the approved digestion methods described in regulation (eu) no. 2015/1375. in the past, trichin ... | 2016 | 27436490 |
| in vitro alterations of erythrocyte aggregation by action of trichinella spiralis newborn larvae. | possible changes in the erythrocyte membrane, by in vitro interaction with newborn larvae of t. spiralis (nl), were evaluated analyzing the alterations in erythrocyte aggregation by digital image analysis and laser transmission in a new optical chip aggregometer. nl were obtained from cbi mice infected with t. spiralis. rbcs samples from healthy donors where in vitro exposed to nl (concentration (3000 ± 500) larvae/ml) to assess its effect on rbc aggregation. individual cell coefficient (cca) an ... | 2017 | 27447422 |
| developmental profile of select immune cells in mice infected with trichinella spiralis during the intestinal phase. | trichinella spiralis can cause immunosuppression during the intestinal phase of early infection. however, changes in the peripheral blood during t. spiralis early infection remain unclear. here, select immune cells in mice infected with 500 muscle larvae (ml) of t. spiralis during the intestinal phase of infection were studied. first, the recovery rates of the intestinal worms and female fecundity were determined, and the results showed that the intestinal worms were completely eliminated at 17 ... | 2016 | 27501987 |
| the roles of supernatant of macrophage treated by excretory-secretory products from muscle larvae of trichinella spiralis on the differentiation of c2c12 myoblasts. | the excretory-secretory products (esps) released by the muscle-larvae (ml) stage of trichinella spiralis have been suggested to be involved in nurse cell formation. however, the molecular mechanisms by which ml-esps modulate nurse cell formation remain unclear. macrophages exert either beneficial or deleterious effects on tissue repair, depending on their activation/polarization state. they are crucial for skeletal muscle repair, notably, via their actions on myogenic precursor cells. however, t ... | 2016 | 27501988 |
| carbonic anhydrase enzymes regulate mast cell-mediated inflammation. | type 2 cytokine responses are necessary for the development of protective immunity to helminth parasites but also cause the inflammation associated with allergies and asthma. recent studies have found that peripheral hematopoietic progenitor cells contribute to type 2 cytokine-mediated inflammation through their enhanced ability to develop into mast cells. in this study, we show that carbonic anhydrase (car) enzymes are up-regulated in type 2-associated progenitor cells and demonstrate that car ... | 2016 | 27526715 |
| p2x7 receptor-dependent tuning of gut epithelial responses to infection. | infection and injury of the gut are associated with cell damage and release of molecules such as extracellular adenosine 5'-triphosphate (atp), which is recognised by the purinergic p2x7 receptor (p2x7r). p2x7r is widely expressed in the gut by antigen-presenting cells (apcs) and epithelial cells, but the role of the p2x7r on epithelial cells is poorly understood. we investigated p2x7r in intestinal epithelium in vitro and in vivo using two model infections, toxoplasma gondii and trichinella spi ... | 2017 | 27559003 |
| [effect of trichinella spiralis and its worm-derived proteins on clp-induced sepsis in mice]. | to observe the effect of trichinella spiralis and its worm-derived proteins on cecal ligation and puncture (clp)-induced sepsis in mice. | 2016 | 27578571 |
| nitazoxanide anthelmintic activity against the enteral and parenteral phases of trichinellosis in experimentally infected rats. | most of the drugs used for the treatment of trichinellosis show a limited bioavailability and a high degree of resistance. therefore, this study aimed to characterize the anthelmintic potential activity of nitazoxanide (ntz) in a rat model of experimental trichinellosis. animals were divided into three groups; group i, infected and non-treated; group ii, received ntz for three days post-infection (dpi) and group iii, received ntz 30 dpi for 14 consecutive days. treatment efficacy was assessed by ... | 2016 | 27585500 |
| oral vaccination with attenuated salmonella typhimurium-delivered tspmy dna vaccine elicits protective immunity against trichinella spiralis in balb/c mice. | our previous studies showed that trichinella spiralis paramyosin (tspmy) is an immunomodulatory protein that inhibits complement c1q and c8/c9 to evade host complement attack. vaccination with recombinant tspmy protein induced protective immunity against t. spiralis larval challenge. due to the difficulty in producing tspmy as a soluble recombinant protein, we prepared a dna vaccine as an alternative approach in order to elicit a robust immunity against trichinella infection. | 2016 | 27589591 |
| survey of trichinella infection from domestic pigs in the historical endemic areas of henan province, central china. | the aim of this work was to investigate the current situation of trichinella infection from domestic pigs in the historical endemic areas of henan province, central china. a total of 823 diaphragm samples from the indoor-raised pigs were collected in five cities of henan during 2014-2015 and examined by artificial digestion method. the overall prevalence of trichinella infection in pigs was 0.61 % (5/823). trichinella larvae were detected in 0.91 % (5/550) of pigs from nanyang city of henan. the ... | 2016 | 27601238 |
| invasion by trichinella spiralis infective larvae affects the levels of inflammatory cytokines in intestinal epithelial cells in vitro. | as we all know, invasion of host intestinal epithelium is very important for t. spiralis to complete successfully their life cycle. however, the mechanisms that the intestinal infective larvae (iil) invade and migrate in the intestinal epithailial cells (iecs) remain unclear until now. the related researches have been hindered since a readily operable in vitro normal model. in our earlier study, an in vitro normal iec invasion model was established for the first time, and the abilities of the no ... | 2016 | 27717772 |
| serological evidence of exposure to globally relevant zoonotic parasites in the estonian population. | we investigated estonian population and its selected subgroups for serological evidence of exposure to ascaris lumbricoides, echinococcus spp., taenia solium, toxocara canis, toxoplasma gondii, and trichinella spiralis. serum samples from 999 adults representing general population, 248 children aged 14-18, 158 veterinarians, 375 animal caretakers, and 144 hunters were tested for specific immunoglobulin g antibodies against the selected parasites using commercial enzyme immunoassays (elisa). sera ... | 2016 | 27723790 |
| nonencapsulated trichinella pseudospiralis infection impairs follicular helper t cell differentiation with subclass-selective decreases in antibody responses. | infectious microorganisms often modify host immunity to escape from immune elimination. trichinella is a unique nematode of the helminth family, whose members parasitize the muscle cells inside the host without robust eliminative reactions. there are several species of trichinella; some develop in muscle cells that become encapsulated (e.g., trichinella spiralis) and others in cells that do not encapsulate (e.g., trichinella pseudospiralis). it has already been established that trichinella infec ... | 2016 | 27736779 |
| resiniferatoxin lowers tnf-α, no and pge2 in the intestinal phase and the parasite burden in the muscular phase of trichinella spiralis infection. | during the course of infection with trichinella spiralis, an inflammatory response is triggered at the intestinal level in the host, playing a crucial role in the expulsion and elimination of the parasite. however, several studies have demonstrated that this inflammatory response is harmful to the host; hence, the importance of studying molecules with therapeutic potential like resiniferatoxin, which is known to have an anti-inflammatory effect both in vitro and in vivo. in this article, we eval ... | 2017 | 27743486 |
| first findings of trichinella spiralis and dna of echinococcus multilocularis in wild raccoon dogs in the netherlands. | the recent invasion of the raccoon dog in the netherlands may be associated with the risk of introduction and spread of zoonotic pathogens. the aim of this study was to assess whether echinococcus multilocularis and trichinella spp. infections are present in dutch raccoon dogs. between 2013 and 2014, nine raccoon dogs, mainly road kills, were collected for necropsies. one raccoon dog tested repeatedly positive in the qpcr for e. multilocularis. the positive raccoon dog was collected in the provi ... | 2016 | 27747155 |
| proteomic analysis of trichinella spiralis adult worm excretory-secretory proteins recognized by early infection sera. | at the intestinal stage of a trichinella spiralis (t. spiralis) infection, the excretory-secretory (es) antigens produced by adult worms (aws) result in an early exposure to the host's immune system and elicit the production of specific antibodies; the aw es proteins might provide early diagnostic markers of trichinellosis. the aim of this study was to identify early serodiagnostic markers from t. spiralis aw es antigens. t. spiralis aws were collected at 72h post infection, and their es antigen ... | 2016 | 27760716 |
| immunomodulatory potential of particular trichinella spiralis muscle larvae excretory-secretory components. | excretory-secretory antigens of trichinella spiralis muscle larvae can induce the semi-matured status of rat dendritic cells. this may at least partly be the consequence of transient activation of extracellular signal-regulated kinases 1/2 (erk1/2). here we investigated the potential of several components of excretory-secretory antigens (native fraction containing 45, 49 and 53kda proteins and recombinant tsp53, representing one of the constituents of this fraction) to demonstrate previously obs ... | 2016 | 27773519 |
| development of an immunodiagnosis method using recombinant pscp for detection of paragonimus skrjabini infection in human. | paragonimiasis skrjabini is a kind of zoonosis and prevalent in 16 provinces in china, such as chongqing, fujian, sichuan, and yunnan. however, sensitive and efficient diagnostic methods for the infection are limited. in order to provide a more convenient and simple method for serologic diagnosis, the recombinant p. skrjabini cysteine protease (pscp) was expressed, purified, and then used to develop an indirect enzyme-linked immunosorbent assay (elisa) for detecting anti-pscp antibodies in human ... | 2017 | 27796563 |
| anti-arthritic activity of schistosoma mansoni and trichinella spiralis derived-antigens in adjuvant arthritis in rats: role of foxp3+ treg cells. | a growing body of evidence supports the concept of helminths therapy in a variety of autoimmune diseases. here, we aimed to investigate the protective effects of autoclaved schistosoma mansoni antigen (asma) and trichinella spiralis antigen (atsa) on the clinical and immunopathological features of rheumatoid arthritis (ra). adjuvant arthritis was induced by subcutaneous and intradermal injections of complete freund's adjuvant into the plantar surface of the right hind paw and the root of the tai ... | 2016 | 27802332 |
| trichinella spiralis paramyosin activates mouse bone marrow-derived dendritic cells and induces regulatory t cells. | dendritic cells (dcs) are antigen-presenting cells that regulate t cell responses for many infectious diseases. the tissue-dwelling nematode trichinella spiralis expresses paramyosin (tspmy) not only as a structural protein but also as an immunomodulator to alleviate complement attack by binding to some host complement components. whether tspmy is involved in other immunomodulatory pathway and how tspmy interacts with host dcs is still unknown. | 2016 | 27809931 |
| immune correlates of resistance to trichinella spiralis reinfection in mice. | the immune correlate of host resistance induced by reinfection of trichinella spiralis remains unclear. in this study, we investigated immune correlates between the resistance and serum igg antibody level, cd23(+) igm(+) b cells, and eosinophil responses induced by t. spiralis reinfection. mice were primarily infected with 10 or 100 t. spiralis larvae (10 ts, 100 ts), respectively, and after 4 weeks, they were challenge infected with 100 t. spiralis larvae (10-100 ts, 100-100 ts). upon challenge ... | 2016 | 27853121 |
| first report of the occurrence of trichinella-specific antibodies in domestic pigs in central and eastern uganda. | previous research on trichinellosis in africa focused on isolating trichinella from wildlife while the role of domestic pigs has remained highly under-researched. pig keeping in uganda is historically recent, and evidence on zoonotic pig diseases, including infection with trichinella species, is scarce. a cross-sectional survey on trichinella seroprevalence in pigs was conducted in three districts in central and eastern uganda from april 2013 to january 2015. serum from a random sample of 1125 p ... | 2016 | 27870858 |
| ubiquitin-dependent modification of skeletal muscle by the parasitic nematode, trichinella spiralis. | trichinella spiralis is a muscle-specific parasitic worm that is uniquely intracellular. t. spiralis reprograms terminally differentiated skeletal muscle cells causing them to de-differentiate and re-enter the cell cycle, a process that cannot occur naturally in mammalian skeletal muscle cells, but one that holds great therapeutic potential. although the host ubiquitin pathway is a common target for viruses and bacteria during infection, its role in parasite pathogenesis has been largely overloo ... | 2016 | 27870901 |
| albendazole microcrystal formulations based on chitosan and cellulose derivatives: physicochemical characterization and in vitro parasiticidal activity in trichinella spiralis adult worms. | the oral route has notable advantages to administering dosage forms. one of the most important questions to solve is the poor solubility of most drugs which produces low bioavailability and delivery problems, a major challenge for the pharmaceutical industry. albendazole is a benzimidazole carbamate extensively used in oral chemotherapy against intestinal parasites, due to its extended spectrum activity and low cost. nevertheless, the main disadvantage is the poor bioavailability due to its very ... | 2016 | 27882479 |
| the induction of the collagen capsule synthesis by trichinella spiralis is closely related to protease-activated receptor 2. | the muscle-stage larvae of the parasite trichinella spiralis have the ability to survive within host muscle tissue by virtue of the formation a nurse cell-parasite complex, which is surrounded by collagen. the formation of the complex is initiated by excretory-secretory (es) proteins produced by the parasite. to determine the mechanisms underlying collagen capsule formation, we investigated the expression levels of several types of collagen genes and tgf-βi signaling-related genes (smad2 and sma ... | 2016 | 27884442 |
| implication of artemisinin nematocidal activity on experimental trichinellosis: in vitro and in vivo studies. | benzimidazole drugs are used for treatment of trichinellosis, but they have a limited effect against encapsulated larval stages of trichinella spiralis. hence, there is a considerable interest in developing new anthelmintic drugs. our aim is to investigate the possible effect of artemisinin on t. spiralis in in vitro and in vivo studies. t. spiralis worms were isolated from infected mice and transferred to 3 culture media; group i: with no drugs, group ii: contained artemisinin and group iii: co ... | 2017 | 27894908 |
| microrna-mediated regulation of immune responses to intestinal helminth infections. | intestinal helminth infections are highly prevalent in the developing world, often resulting in chronic infection and inflicting high host morbidity. with the emergence of drug-resistant parasites, a limited number of chemotherapeutic drugs available and stalling vaccine efforts, an increased understanding of antihelminth immunity is essential to provide new avenues to therapeutic intervention. micrornas are a class of small, nonprotein coding rnas which negatively regulate mrna translation, thu ... | 2017 | 27977850 |
| early detection of trichinella spiralis dna in the feces of experimentally infected mice by using pcr. | the aim of this study was to detect trichinella spiralis dna in mouse feces during the early stages of infection using pcr. the target gene fragment, a 1.6kb repetitive sequence of t. spiralis genome, was amplified by pcr from feces of mice infected with 100 or 300 larvae at 3-24h post infection (hpi) and 2-28dpi. the sensitivity of pcr was 0.016 larvae in feces. the primers used were highly specific for t. spiralis. no cross-reactivity was observed with the dna of other intestinal helminths. t. ... | 2017 | 27983972 |
| piezo2: a candidate biomarker for visceral hypersensitivity in irritable bowel syndrome? | currently, there exists no biomarker for visceral hypersensitivity in irritable bowel syndrome (ibs). piezo proteins have been proven to play an important role in the mechanical stimulation to induce visceral pain in other tissues and may also be a biomarker candidate. the aim of this study was to test the expressions of piezo1 and piezo2 proteins in the intestinal epithelial cells from different intestinal segments and to explore the correlation between piezo proteins expression and visceral pa ... | 2017 | 28044050 |
| immune responses in mice vaccinated with a dna vaccine expressing serine protease-like protein from the new-born larval stage of trichinella spiralis. | trichinella spiralis is a parasitic helminth that can infect almost all mammals, including humans. trichinella spiralis infection elicits a typical type 2 immune responses, while suppresses type 1 immune responses, which is in favour of their parasitism. dna vaccines have been shown to be capable of eliciting balanced cd4+ and cd8+ t cell responses as well as humoral immune responses in small-animal models, which will be advantage to induce protective immune response against helminth infection. ... | 2017 | 28069101 |
| study on the mitochondrial apoptosis pathways of small cell lung cancer h446 cells induced by trichinella spiralis muscle larvae esps. | trichinella spiralis (t.spiralis) muscle-larva (ml) excretory-secretory proteins (esps) contain antitumour-active substances. esps have been shown to inhibit tumour growth. to explore the effects of these proteins on small cell lung cancer cells and the possible mechanisms of their antineoplastic action, h446 sclc cells were co-cultured with different concentrations of t. spiralis ml esps for 12, 24 and 48 h. our results showed that t. spiralis ml esps significantly inhibited h446 cell prolifera ... | 2017 | 28073393 |
| paraoxonase-1 activity is related to trichinella spiralis-induced hepatitis in rats. | little is known about the potential adverse effects of a chronic zoonotic nematode trichinella spiralis infection on hepatic inflammation and its relationship to paraoxonase (pon)-1 and butyrylcholinesterase (buche) activities. therefore, we aimed to examine the effects of t. spiralis infection on hepatic synthesis of pon1. | 2017 | 28103386 |
| t-cell clones in human trichinellosis: evidence for a mixed th1/th2 response. | in humans, studies on the cellular immune response against trichinella are scarce. aim of this study was to characterize the cytokine profile of t cells specific for trichinella britovi in trichinellosis patients. peripheral blood mononuclear cells (pbmc) were obtained from five patients involved in a trichinellosis outbreak caused by t. britovi, which occurred in 2013 in tuscany (italy). all the patients resulted positive for trichinella-specific igg, ige and presented eosinophilia. t cells wer ... | 2017 | 28106258 |
| experimental arthritis is dependent on mouse mast cell protease-5. | the constitutive heparin(+) (hp) mast cells (mcs) in mice express mouse mc protease (mmcp)-5 and carboxypeptidase a (mmc-cpa). the amino acid sequence of mmcp-5 is most similar to that of human chymase-1, as are the nucleotide sequences of their genes and transcripts. using a homologous recombination approach, a c57bl/6 mouse line was created that possessed a disrupted mmcp-5 gene. the resulting mice were fertile and had no obvious developmental abnormality. lack of mmcp-5 protein did not alter ... | 2017 | 28193842 |
| new insights on serodiagnosis of trichinellosis during window period: early diagnostic antigens from trichinella spiralis intestinal worms. | the clinical diagnosis of trichinellosis is difficult because its clinical manifestations are nonspecific. detection of anti-trichinella igg by elisa using t. spiralis muscle larval excretory-secretory (es) antigens is the most commonly used serological method for diagnosis of trichinellosis, but the main disadvantage is false negativity during the early stage of infection. there is an obvious window period between trichinella infection and antibody positivity.during the intestinal stage of tric ... | 2017 | 28219418 |
| identification and characterization of conserved mirnas with its targets mrna in trichinella spiralis. | micrornas (small regulatory non-coding rnas) have an important role in gene regulation and evolutionarily conserved molecules. trichinella spiralis infect majority of species. therefore, it is of interest to identify conserved mirnas and their targets using sequences from est, gss and full length nucleotides obtained from ncbi against previously reported worm mirnas. we identify 11 novel mirnas in t. spiralis by using bioinformatics-homology based search. in addition, we predicted target mrna ge ... | 2016 | 28246461 |
| a systematic review of taeniasis, cysticercosis and trichinellosis in vietnam. | taeniasis, cysticercosis and trichinellosis have been ranked as the most important food-borne parasites of humans in terms of public health, socioeconomic and trade impact. despite this, information on these food-borne zoonoses in vietnam is scarce and fragmented, and many local reports remain inaccessible to the international research community. this study aims to conduct comprehensive literature searches to report on the incidence and estimate the true prevalence of taeniasis in humans and t. ... | 2017 | 28320455 |
| tspka-r: a potential immunodiagnostic antigen for the detection of porcine cysticercosis. | cysticercosis, caused by metacestodes of taenia solium, has a significant soci-economic impact and is of considerable importance in public health. however, there are no specific diagnostic antigens to distinguish between t. solim and taenia hydatigena. in the present study, camp-dependent protein kinase regulatory subunit (tspka-r), an excretory/secretary (es) antigen of t. solium, was used to establish a specific and sensitive diagnostic tool for detection of porcine cysticercosis. the full-len ... | 2017 | 28359828 |
| innate immunity modulation in the duodenal mucosa induced by rem sleep deprivation during infection with trichinella spirallis. | sleep is considered to be an important predictor of the immunity, since the absence of sleep can affect the development of the immune response, and consequently increase the susceptibility to contract an infection. the aim of the present study was to investigate if sleep deprivation and stress induce dysregulation of the duodenal mucous membrane during the acute infection with trichinella spiralis. our results shows that, in the intestinal mucous membrane, stress and sleep deprivation, produces ... | 2017 | 28374797 |
| t-bet independent development of ifnγ secreting natural t helper 1 cell population in the absence of itk. | th1, th2, th9 and th17 cells are conventional cd4(+) effector t cells identified as secretors of prototypical cytokines ifnγ, il4, il9, and il-17a respectively. recently, populations of natural th17 and th1 cells (nth17 and nth1) with innate-like phenotype have been identified in the thymus that are distinct from conventional th17 and th1 cells. the absence of the tec family kinase interleukin-2 inducible t cell kinase (itk) results in t cell immunodeficiency in mice and humans. here we show tha ... | 2017 | 28406139 |
| influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from trichinella spiralis. | nematodes of the genus trichinella are one of the most widespread zoonotic pathogens on the world, and they can still cause major public health problems in many parts of the world. vaccination against the helminth nematode trichinella could be a good strategy to reduce the risk of human and animal infection. it was our aim to evaluate three adjuvants, which could be used as an efficient vaccine for animals in combination with rts-serpin antigen. in this study, balb/c mice were vaccinated by an i ... | 2017 | 28445612 |
| trichinella spiralis impact on mesenchymal stem cells: immunohistochemical study by image analyzer in murine model. | this study aims to elucidate whether trichinella spiralis infection or its crude antigen administration can stimulate recruitment of cd105(+ve)/cd45(-ve) cells that could represent mscs in intestine and skeletal muscle of experimental balb/c albino mice compared to healthy control mice. studied mice were divided into: 20 healthy control, 20 with orally induced t. spiralis infection, 20 received adult worm crude antigen orally and 20 received larval crude antigen intramuscular. according to speci ... | 2017 | 28456661 |
| oral contraceptive pills: risky or protective in case of trichinella spiralis infection? | the aim of this study was to investigate how trichinella spiralis infection can be affected by contraceptive pills in vivo. methods included six groups of female wistar rats; healthy, trichinella infected, receiving combined contraceptive pills (cocps), receiving progestin only pills (pops), infected receiving cocps and infected receiving pops. parasite burden was measured; adult worm counts, gravidity, larvae and reproductive capacity index). histopathological examination, immunohistochemical d ... | 2017 | 28524239 |
| rifaximin exerts beneficial effects in pi-ibs mouse model beyond gut microbiota. | rifaximin is a minimally absorbed antibiotic which has shown efficacy in irritable bowel syndrome (ibs) patients. however, the mechanism how it effects in ibs is still incompletely defined. in this study, we used trichinella spiralis infected pi-ibs mouse model, to assess the action of rifaximin on visceral hypersensitivity, barrier function, gut inflammation and microbiota. | 2017 | 28573746 |
| resiniferatoxin modulates the th1 immune response and protects the host during intestinal nematode infection. | in the early stage of the intestinal phase of trichinella spiralis infection, the host triggers a th1-type immune response with the aim of eliminating the parasite. however, this response damages the host which favours the survival of the parasite. in the search for novel pharmacological strategies that inhibit the th1 immune response and assist the host against t. spiralis infection, a recent study showed that resiniferatoxin had anti-inflammatory activity contributed to the host in t. spiralis ... | 2017 | 28580764 |
| long-read sequencing improves assembly of trichinella genomes 10-fold, revealing substantial synteny between lineages diverged over 7 million years. | genome assemblies can form the basis of comparative analyses fostering insight into the evolutionary genetics of a parasite's pathogenicity, host-pathogen interactions, environmental constraints and invasion biology; however, the length and complexity of many parasite genomes has hampered the development of well-resolved assemblies. in order to improve trichinella genome assemblies, the genome of the sylvatic encapsulated species trichinella murrelli was sequenced using third-generation, long-re ... | 2017 | 28583210 |
| proteomic analysis of trichinella spiralis adult worm excretory-secretory proteins recognized by sera of patients with early trichinellosis. | the most commonly used serodiagnostic antigens for trichinellosis are the excretory-secretory (es) antigens from t. spiralis muscle larvae (ml), but the specific antibodies against the ml es antigens are usually negative during early stage of trichinella infection. the recent studies demonstrated that t. spiralis adult worm (aw) antigens were recognized by mouse or swine infection sera on western blot as early as 7-15 days post-infection (dpi), the aw antigens might contain the early diagnostic ... | 2017 | 28620363 |
| trichinella spiralis calreticulin binds human complement c1q as an immune evasion strategy. | as a multicellular parasitic nematode, trichinella spiralis regulates host immune responses by producing a variety of immunomodulatory molecules to escape from host immune attack, but the mechanisms underlying the immune evasion are not well understood. here, we identified that t. spiralis calreticulin (ts-crt), a ca(2+)-binding protein, facilitated t. spiralis immune evasion by interacting with the first component of human classical complement pathway, c1q. in the present study, ts-crt was foun ... | 2017 | 28620388 |
| studies on vertical transmission of trichinella spiralis in experimentally infected guinea pigs (cavia porcellus). | an experimental study to enhance knowledge on the capability of trichenella spiralis to pass from guinea pigs to progeny at different periods of pregnancy or lactation was performed. for this purpose, 18 female adult guinea pigs were inoculated with 100 or 1000 t. spiralis muscle larvae (ml) during early, late gestation and during lactation period. the presence of t. spiralis (ml) in mothers and newborns was studied through enzymatic digestion from muscle samples. ml were observed in 9 of 42 new ... | 2017 | 28631164 |
| [a hundred years since the beginning of the great war: trichinosis outbreak in dresden marine in chile: chronicle of an announced infection]. | in commemoration of one hundred years of the beginning of world war i, the occurrence of an outbreak of trichinosis in the crew of the sms dresden, a german ship that participated in that world conflagration, is re-viewed. in september 1915, while the sailors of sms dresden were forcibly detained on isla quiriquina, bío bío region, chile, 60 individuals became ill. the cause of the outbreak was the consumption of sausages made from pork infected with the parasite trichinella spiralis. there was ... | 2017 | 28632832 |
| therapeutic potential of helminths in autoimmune diseases: helminth-derived immune-regulators and immune balance. | helminths have accompanied human throughout history by releasing immune-evasion molecules that could counteract an aberrant immune response within the host. in the past decades, helminth infections are becoming less prevalent possibly due to the developed sanitation. meanwhile, the incidence of autoimmune diseases is increasing, which cannot be exclusively explained by the changes of susceptibility genes. while the hygiene hypothesis casts light on the problem. the infections of helminths are be ... | 2017 | 28664463 |
| development and evaluation of a rapid diagnostic immunochromatographic device to detect antibodies in sera from intestinal capillariasis cases. | intestinal capillariasis, a fish-borne nematodiasis, is an important emerging zoonotic disease. patients present clinical symptoms of borborygmus chronic diarrhea, intermittent abdominal pain, weight loss, and several degrees of painless lower-leg edema. death may occur in cases of misdiagnosis and improper treatment. diagnosis is difficult because of the atypical clinical symptoms and diagnostic confusion with diarrhea caused by gastrointestinal cancer, opportunistic infections in human immunod ... | 2017 | 28667523 |
| reduced interstitial cells of cajal and increased intraepithelial lymphocytes are associated with development of small intestinal bacterial overgrowth in post-infectious ibs mouse model. | intestinal dysmotility and immune activation are likely involved in the pathogenesis of small intestinal bacteria overgrowth (sibo) in irritable bowel syndrome (ibs). we aimed at investigating the role of interstitial cells of cajal (icc) and intestinal inflammation in the development of sibo using a post-infectious ibs (pi-ibs) mouse model. | 2017 | 28679338 |
| antibodies to various zoonotic pathogens detected in feral swine (sus scrofa) at abattoirs in texas, usa. | the zoonotic risk posed to employees by slaughtering feral swine (sus scrofa) at two abattoirs in texas was assessed by testing feral swine serum samples for exposure to influenza a virus, leptospira, trichinella spiralis, and toxoplasma gondii. blood was collected from a total of 376 feral swine between the two facilities during six separate collection periods in 2015. antibodies to one or more serovars of leptospira were identified in 48.9% of feral swine tested, with bratislava and pomona as ... | 2017 | 28686494 |
| intraperitoneal inoculation: an atypical route of trichinella spiralis infection. | the intraperitoneal injection is a common method for establishing the experimental animal model infected with parasites. the aim of this study was to investigate if the intraperitoneal injection was another route of trichinella spiralis infection. | 2017 | 28761470 |
| m-rna gene expression of inf-γ and il-10 during intestinal phase of trichinella spiralis after myrrh and albendazole treatment. | the protective response developed against trichinella spiralis infection provokes immune and inflammatory responses mediated by cytokines released from t helper cells. we aimed to evaluate the effect of albendazole or myrrh on the expression of ifn-γ and il-10 in balb/c mice infected with t. spiralis. | 2017 | 28761478 |
| heterologous prime-boost vaccination enhances tspmy's protective immunity against trichinella spiralis infection in a murine model. | tspmy is a paramyosin expressed by parasitic trichinella spiralis and confers a protective immunity when its recombinant protein or dna was used as an immunogen. to improve its immunogenicity and vaccine efficacy, we conducted a heterologous prime-boost strategy by orally delivering one dose of tspmy dna carried by attenuated salmonella typhimurium (sl7207), followed by two doses of recombinant tspmy intramuscularly. this strategy effectively induced intestinal mucosal siga response and an enhan ... | 2017 | 28785255 |
| evaluation of the priocheck™ trichinella aad kit for the digestion and recovery of larvae in pork, horse meat and wild meat. | the artificial digestion magnetic stirrer method using pepsin protease and hydrochloric acid is the standard assay for the detection of trichinella larvae in muscle of infected animals. recently, an alternative enzyme, serine protease, was employed in the development of a commercially available digestion kit (priocheck™ trichinella aad kit). this assay requires a higher digestion temperature of 60°c which kills the larvae during the digestion process, mitigating the risk of environmental contami ... | 2017 | 28807305 |
| vaccination with a paramyosin-based multi-epitope vaccine elicits significant protective immunity against trichinella spiralis infection in mice. | trichinellosis is a worldwide zoonosis and remains a serious public health problem. interrupting parasite transmission via vaccination of livestocks with a potent vaccine is a practical approach to prevent human trichinellosis. our previous studies have identified that paramyosin of trichinella spiralis (ts-pmy) is a good vaccine candidate against trichinellosis. in this study, a novel multi-epitope vaccine (mep) was constructed by using four cd4(+) t cell epitopes (p2, p3, p4, and p5) and one b ... | 2017 | 28824599 |
| crystal structures of nematode (parasitic t. spiralis and free living c. elegans), compared to mammalian, thymidylate synthases (ts). molecular docking and molecular dynamics simulations in search for nematode-specific inhibitors of ts. | three crystal structures are presented of nematode thymidylate synthases (ts), including caenorhabditis elegans (ce) enzyme without ligands and its ternary complex with dump and raltitrexed, and binary complex of trichinella spiralis (ts) enzyme with dump. in search of differences potentially relevant for the development of species-specific inhibitors of the nematode enzyme, a comparison was made of the present ce and ts enzyme structures, as well as binary complex of ce enzyme with dump, with t ... | 2017 | 28826032 |
| single cell phenotyping reveals heterogeneity among haematopoietic stem cells following infection. | the haematopoietic stem cell (hsc) niche provides essential micro-environmental cues for the production and maintenance of hscs within the bone marrow. during inflammation, haematopoietic dynamics are perturbed, but it is not known whether changes to the hsc-niche interaction occur as a result. we visualise hscs directly in vivo, enabling detailed analysis of the 3d niche dynamics and migration patterns in murine bone marrow following trichinella spiralis infection. spatial statistical analysis ... | 2017 | 28833970 |
| [nuclease activity of the recombinant plancitoxin-1-like proteins with mutations in the active site from trichinella spiralis]. | although there are 125 predicted dnase ⅱ-like family genes in the trichinella spiralis genome, plancitoxin-1-like (ts-pt) contains the hkd motif, a typical conserved region of dnase ⅱ, in n- and c-terminal. it is generally believed that histidine is the active site in dnase ⅱ. to study the nuclease activity of recombinant ts-pt with mutations in the active site from t. spiralis, different fragments of the mutated ts-pt genes were cloned using overlap pcr technique and inserted into the expressin ... | 2017 | 28853259 |