Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| sequence of the 5.8s ribosomal gene of pathogenic and non-pathogenic isolates of entamoeba histolytica. | 1993 | 8441643 | |
| determination of the pathogenicity of entamoeba histolytica. | 1993 | 8443325 | |
| molecular cloning of a rho family gene of entamoeba histolytica. | an entamoeba histolytica gene (eh rho1) was cloned that encodes a putative low-molecular-mass gtp-binding protein, most similar to the ras homologue rho. the eh rho1 open reading frame was 208 amino acids long and encoded a 23-kda protein similar to saccharomyces cerevisiae rho1-rho4 and cdc42 and human rhoa, rac1, and g25k gene products. this similarity was greatest at the nh2 terminus of eh rho1 where two gtp-binding sites and a possible effector site were conserved. a cysteine residue at the ... | 1993 | 8459832 |
| cloning of a virulence factor of entamoeba histolytica. pathogenic strains possess a unique cysteine proteinase gene. | cysteine proteinases are hypothesized to be important virulence factors of entamoeba histolytica, the causative agent of amebic dysentery and liver abscesses. the release of a histolytic cysteine proteinase from e. histolytica correlates with the pathogenicity of both axenic strains and recent clinical isolates as determined by clinical history of invasive disease, zymodeme analysis, and cytopathic effect. we now show that pathogenic isolates have a unique cysteine proteinase gene (acp1). two ot ... | 1993 | 8473498 |
| interaction between trophozoites of entamoeba histolytica and the human intestinal cell line ht-29 in the presence or absence of leukocytes. | studies on the interaction between trophozoites of entamoeba histolytica of pathogenic or non-pathogenic origin and epithelial cells of the human intestine can contribute to the understanding of the pathogenesis of invasive amoebiasis. we have examined the interaction of virulent e. histolytica with the human colonic carcinoma cell line ht-29. differentiated ht-29 cells are comparable to the mucosa cells to which e. histolytica attaches physiologically. adherence between e. histolytica trophozoi ... | 1993 | 8475032 |
| expression of sequences related to c-myc in entamoeba. | the evolutionarily conserved proto-oncogene c-myc is involved in both proliferation and differentiation processes of higher eukaryotic cells. we report here the identification and characterization of sequences homologous to c-myc in different entamoeba species using a fragment of the mammalian c-myc gene as a probe. this probe hybridized with fragments of 3.5 and 3.4 kilobases (kb) in e. histolytica hindiii of ecori digested dna. in e. invadens it recognized fragments of 3.1 and 2.8 kb, and in l ... | 1993 | 8475034 |
| phagocytosis and proteinase activity are not related to pathogenicity of e. histolytica. | to examine the relationship between phagocytosis, proteinase activity and pathogenicity of axenically grown trophozoites of e. histolytica strain hm-1:imss four different cultures were used: (1) a culture preserved in our laboratory for over 4 years, which lost its pathogenicity 3 years ago; (2) a culture passaged several times through hamster liver, which lost its pathogenicity recently; (3) a highly virulent culture supplied by another laboratory; and (4) amebas recovered from hamster liver ab ... | 1993 | 8475035 |
| proteinases resistant to sodium dodecyl sulfate-induced autoproteolysis of entamoeba histolytica hk9 trophozoites. | 1993 | 8475037 | |
| complement resistance of pathogenic entamoeba histolytica mediated by trypsin-sensitive surface component(s). | pathogenic forms of the protozoan parasite entamoeba histolytica were reported previously to resist the cytolytic effect of the alternative complement pathway (ap) only temporarily during exposure to complement. in contrast, nonpathogenic forms of e. histolytica had been found to show ap resistance as a stable property. we studied the mechanisms of ap resistance of the two forms. upon exposure to ap activity, resistant pathogenic or nonpathogenic forms bound significantly less c3 products than c ... | 1993 | 8478051 |
| use of a high molecular weight entamoeba histolytica antigen fraction in enzyme-linked immunosorbent assay and thin layer immunoassay tests. | a high molecular weight entamoeba histolytica antigen fraction i was employed for the determination of anti-amoebic antibodies in elisa and thin layer immunoassay (tia) tests. a large number of human serum and immunized guinea pig serum samples were tested against this antigen. in comparison, elisa was found more sensitive than the tia technique. the potent antigen fraction i used in these tests was lyophilized at an optimum concentration of 1 mg/ml for obtaining uniform results. | 1993 | 8481651 |
| nucleotide sequence analysis of the rrna transcription unit of a pathogenic entamoeba histolytica strain hm-1:imss. | 1993 | 8493112 | |
| entamoeba histolytica cysts with a defective wall formed under axenic conditions. | axenic hk9 entamoeba histolytica strain amoebae, maintained in pehs medium, displayed several cystic characteristics that involve an active process of cystic wall formation, cellular volume and density diminution, and one or two nuclear divisions. the differentiation process was asynchronic, beginning after the logarithmic growth phase. the axenic cysts, which were maintained in a 50 mosm/kg medium at 4 degrees c for 72 h, produced growing trophozoites within 1-7 days of incubation at 36 degrees ... | 1993 | 8493243 |
| a human cdna corresponding to a gene overexpressed during cell proliferation encodes a product sharing homology with amoebic and bacterial proteins. | a clone, designated pag, was isolated by differential screening of cdna libraries made from the untransformed and ras-transformed human mammary epithelial cell line hbl100. this cdna corresponds to a gene constitutively expressed in most human cells which is induced to higher levels upon serum stimulation in untransformed and ras-transformed hbl100 cells. however, the abundance of the pag transcript is approximately 3-fold higher in transformed as compared to untransformed cells after 7-15 h of ... | 1993 | 8496166 |
| entamoeba histolytica trophozoites: a surface-associated cysteine protease. | the purpose of this work was to define cell surface proteases on entamoeba histolytica trophozoites. molecular sizes of 2-mercaptoethanol-activated proteases were determined in several ameba cell fractions. inhibited proteases were resolved in sds-polyacrylamide gels and then defined by their ability to digest bovine albumin during electrophoretic migration of ameba bands through a stacking gel containing albumin. this second gel revealed nine gaps of digestion along the horizontal albumin line ... | 1993 | 8500583 |
| cloning of the eh cdc2 gene from entamoeba histolytica encoding a protein kinase p34cdc2 homologue. | to understand better growth regulation in the protozoan parasite, entamoeba histolytica (eh), a homologue of the cdc2 gene encoding the yeast cyclin-dependent protein kinase, p34cdc2, has been cloned and sequenced. this gene, called eh cdc2, contains a 79-bp intron located in the same place as the second of four introns in the schizosaccharomyces pombe cdc2 gene. the sequence of an eh cdc2 cdna confirms the conserved eukaryotic splice donor (gt) and acceptor (ag) sites and shows that eh is able ... | 1993 | 8500762 |
| in vitro effect of 5-hydroxytryptamine on concanavalin-a agglutinability and multiplication of entamoeba histolytica. | six isolates of e. histolytica isolated and maintained by serial passage in modified boeck and drbohlav's medium were used in this study. when five of the isolates were grown in the above medium with 5-hydroxytryptamine (5-ht) added to the overlay, the agglutinability of the amoebae by concanavalin- a (con-a) was significantly increased compared to corresponding control cultures. four isolates of e. histolytica grown with 5-ht had 1.5 to 2 times higher counts than the control cultures. similarly ... | 1993 | 8500845 |
| trichuris vulpis infection in an indian tribal population. | stools from 28 of the 82 inhabitants on remote little andaman island in india were examined for parasite eggs and cysts. trichuris trichiura eggs were found in 27, trichuris vulpis eggs in 5, strongyloides stercoralis larvae in 3, hookworm eggs in 15, entamoeba histolytica and entamoeba coli cysts each in 9, giardia lamblia in 6, retortamonas sp. in 3, iodoamoeba sp. in 2, and chilomastix sp. in 2 stools. ascaris lumbricoides eggs were not seen. the occurrence of t. vulpis eggs in 5 stools and t ... | 1993 | 8501609 |
| ulcerative colitis and antineutrophil cytoplasmic antibodies in hong kong chinese. | inflammatory bowel diseases are known to be rare among the chinese. the diagnosis of ulcerative colitis has been difficult in some of the asian countries where infective colitis is more prevalent. twenty-three hong kong chinese patients diagnosed to have ulcerative colitis were reviewed. the symptoms were relatively mild and extraintestinal manifestation had been rare. patients responded well to steroid therapy and sulfasalazine. three patients in this series were found to have cyst and/or troph ... | 1993 | 8503382 |
| induction of complement resistance in cloned pathogenic entamoeba histolytica. | the lytic effect of complement activated through the alternative pathway (ap) was studied on pathogenic and nonpathogenic entamoeba histolytica recently isolated from stool samples. recent nonpathogenic isolates were nearly unaffected by exposure to ap whereas recent pathogenic stool isolates were highly susceptible to ap dependent complement-mediated lysis. complement susceptible pathogenic stool isolates developed complement resistance in vivo during hamster liver passage and in vitro during c ... | 1993 | 8506118 |
| quassinoids exhibit greater selectivity against plasmodium falciparum than against entamoeba histolytica, giardia intestinalis or toxoplasma gondii in vitro. | the in vitro activities of a series of quassinoids against plasmodium falciparum, entamoeba histolytica, giardia intestinalis and toxoplasma gondii have been compared with their in vitro cytotoxic effects against kb cells (human epidermoid carcinoma of the nasopharynx). all of the compounds tested were more toxic to kb cells than to g. intestinalis, but four (ailanthinone, bruceine d, brusatol and glaucarubinone) were slightly less toxic to kb cells than to e. histolytica. glaucarubinone was sim ... | 1993 | 8508162 |
| a redescription of entamoeba histolytica schaudinn, 1903 (emended walker, 1911) separating it from entamoeba dispar brumpt, 1925. | explaining the low incidence of invasive disease (10%) in humans infected with entamoeba histolytica has occupied the attention of generations of both clinical and nonclinical investigators. one possible explanation would be the existence of two morphologically identical species-one an invasive pathogen, the other noninvasive. this was first proposed by brumpt in 1925, but his explanation was virtually ignored until 1978 when the first of several publications appeared suggesting that e. histolyt ... | 1993 | 8508172 |
| comparison of pore-forming peptides from pathogenic and nonpathogenic entamoeba histolytica. | similar to the findings obtained with pathogenic entamoeba histolytica, nonpathogenic isolates were found to kill mammalian cells in vitro, and cell extract caused pore formation in liposome membranes. a pore-forming peptide termed apnp was isolated from a nonpathogenic isolate using the schedule developed for the purification of app or amoebapore, the homologous peptide of the pathogenic isolate hm-1:imss. compared to app, the specific activity of apnp in pore formation was 60% lower. cdna sequ ... | 1993 | 8515772 |
| identification and characterization of a myosin heavy chain gene (mhca) from the human parasitic pathogen entamoeba histolytica. | the mhca gene from the human pathogen entamoeba histolytica was identified using the polymerase chain reaction. it is a single copy gene expressed as a 6.4-kb mrna. the deduced mhca protein sequence is highly similar to myosin ii from both dictyostelium discoideum and acanthamoeba castellanii. the globular head domain of mhca contains the specific regions involved in atp binding, actin binding, and interaction with myosin light chain. the tail domain is organized in an alpha-helical coiled coil ... | 1993 | 8515774 |
| molecular karyotype of related clones of entamoeba histolytica. | the molecular karyotype of 3 clones derived from strain hm1:imss of entamoeba histolytica was studied by transverse alternating field electrophoresis. 11-20 bands ranging between 0.3 and over 3 mb were resolved. hybridization with total dna detected highly repetitive sequences in the slow-migrating molecules, while non-repetitive sequences were located in the intermediate and fast-migrating molecules. rdna, tubulin, actin, cysteine proteases dna fragments, and a variable dna sequence (ehvr1) loc ... | 1993 | 8515781 |
| a family of transcripts (k2) of entamoeba histolytica contains polymorphic repetitive regions with highly conserved elements. | sera from patients with invasive amebiasis were used to identify a cdna clone (k2p-1) encoding a commonly recognized, repeat-containing antigen of the pathogenic entamoeba histolytica hm-1:imss. k2p-1 was used to isolate 3 cdna clones (k2 clones); one k2p-1 related clone from the same pathogenic e. histolytica strain and 2 from the nonpathogenic e. histolytica strain saw-142. the nucleotide sequence and predicted amino acid sequence revealed a closely related family of transcripts differing main ... | 1993 | 8515783 |
| the basic isoform of profilin in pathogenic entamoeba histolytica. cdna cloning, heterologous expression, and actin-binding properties. | in the human parasite entamoeba histolytica, components of the cytoskeleton are involved in the pathogenicity by their contribution to immune evasion by antibody capping and shedding. in this study, we focus on profilin as a central regulatory component of the cytoskeleton. profilin was isolated from trophozoites of the pathogenic e. histolytica strain sfl-3, and partial amino acid sequences were used to devise a probe for isolating a profilin cdna. the deduced complete primary structure was div ... | 1995 | 8521867 |
| [amebic liver abscess: review of 13 cases]. | amebian hepatic abscess (aha) is the most frequent extraintestinal localization of infection by entamoeba histolytica. despite being a disease mainly of tropical zones, it is currently also observed with higher frequency in spain. thirteen cases of aha diagnosed in the authors' hospital over the last 11 years were retrospectively analyzed comparing the data in this series with that of other published series and undertaking a review of this subject. the diagnosis of aha was established on the bas ... | 1995 | 8523930 |
| intestinal parasites in school food handlers in the city of uberlândia, minas gerais, brazil. | in order to verify the presence of intestinal parasites in food handlers, stool samples were collected from 104 cooks and their helpers that were working in food preparation in 20 public elementary schools, in various areas of the city of uberlândia, minas gerais, brazil. the samples were collected during the months of november and december, 1988, in plastic flasks containing a 10% formaldehyde solution and processed by the hoffmann, pons & janer method. the sediment was examined using triplicat ... | 1995 | 8525262 |
| influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of entamoeba histolytica. | at this moment, the duality of species suggested for e. histolytica is being considered for discussion. in order to contribute to settling this question, we investigated the possibility of conversion of avirulent ameba to virulent ones, as well as, the possibility of increasing virulence of virulent strains, by means of association with bacteria. five strains of e. histolytica were employed, two of them regarded as avirulent and three virulent ones. amebas were associated with the bacteria esche ... | 1995 | 8525263 |
| opportunistic and non-opportunistic parasites in hiv-positive and negative patients with diarrhoea in tanzania. | a survey on intestinal parasites in a rural area of tanzania revealed the presence of eight protozoa and seven helminths in 287 subjects (81.8%). the prevalence of entamoeba histolytica and ascaris lumbricoides was higher in hiv-negative than in hiv-positive patients (p < 0.01; p < 0.04) (25.1% and 12.5% for e. histolytica; 10.5% and 3.7% for a. lumbricoides). on the other hand, cryptosporidium parvum, isospora belli and strongyloides stercoralis prevalence was higher in hiv-positive than in hiv ... | 1995 | 8525281 |
| prevalence of intestinal parasitic infections among asian female house keepers in abha district, saudi arabia. | a group of 5,518 female asian house keepers working in abha district of saudi arabia was examined (1990 through 1992) to determine the prevalence of intestinal parasitic infections. they came from indonesia, sri lanka, philippines and thailand. fresh stool specimens were obtained in special containers and examined by light microscopy of wet smears in normal saline and lugoll's iodine solution within one hour of collection. the study revealed an overall prevalence of 46.5% which was higher than t ... | 1995 | 8525400 |
| entamoeba histolytica adherence: inhibition by iga monoclonal antibodies. | 1995 | 8526018 | |
| a novel transcribed repeat element from entamoeba histolytica. | we have identified an unusual 0.55-kb dna repeat element specific to entamoeba histolytica (eh) which we call interspersed element (ie). the ie is a common feature in independently isolated genomic and cdna fragments. hybridization of labeled ie sequences to trophozoite dna, rna and first-strand cdna prepared from poly(a)-enriched mrna indicate that the ie are reiterated about 500 times per eh trophozoite and that one or more can be found as rna transcripts. these features and the degree of cons ... | 1995 | 8529889 |
| viral diarrhoea in a rural coastal region of karnataka india. | a total of 106 children below 5 years of age admitted to the kasturba medical college hospital manipal karnataka (south india) were investigated over a period of 6 months to determine the aetiological role of viruses in acute diarrhoea. viral aetiological agents isolated were rotaviruses in 12 (11 per cent) cases, adenoviruses in 3 (3 per cent) cases, coronavirus and astroviruses in two (2 per cent) cases each. non-viral isolates were cryptosporidium and salmonella typhimurium in two cases each, ... | 1995 | 8531264 |
| amoebicidal compounds from medicinal plants. | a series of the natural constituents with amoebicidal activity isolated from several medicinal plants is shown. a list of the medicinal plants potentially active as amoebicide and/or against dysentery also is demonstrated. the present data grouping of the natural compounds and medicinal plants can be an important source of information for the selection of research plant material by the investigators interested in the discovery of new biologically active compounds as amoebicide. | 1995 | 8532364 |
| evaluation of three serological tests for the detection of antiamebic antibodies applied to sera of patients from an area endemic for amebiasis. | two enzyme immuno assays based on a single recombinant entamoeba histolytica antigen (p1-eia) or soluble e. histolytica extract (sa-eia) as well as a latex agglutination test using an e. histolytica membrane fraction (m-la) were evaluated for its use to detect anti-amebic serum antibodies in patients from durban, south africa, an area endemic for amebiasis. in a previous study, all three test systems were found to be reliable in terms of sensitivity and specificity when applied to sera of europe ... | 1995 | 8533021 |
| [clinico-epidemiological implications of autochthonous infection caused by entamoeba histolytica]. | 1995 | 8534547 | |
| surface localization, regulation, and biologic properties of the 96-kda alcohol/aldehyde dehydrogenase (ehadh2) of pathogenic entamoeba histolytica. | the 96-kda surface antigen of entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (ehadh2). ehadh2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. of importance, using rnase protection assays, specific mrna coding for the ehadh2 gene product(s) was up-regu ... | 1996 | 8537663 |
| analysis of the 170-kda lectin gene promoter of entamoeba histolytica. | the promoter region driving the gene for the 170-kda heavy subunit of the entamoeba histolytica galactose-inhibitable lectin was analysed by transient transfection using the chloramphenicol acetyltransferase gene as reporter. s1 mapping confirmed our previous notion that the promoter is located within a 1.35-kb intergenic sequence preceding the structural lectin gene. transcripts derived from the chloramphenicol acetyltransferase gene of transfected trophozoites were found to be polyadenylated a ... | 1995 | 8538679 |
| free radical detoxification in giardia duodenalis. | non-denaturing polyacrylamide gels were used to analyse superoxide dismutase (sod), catalase, peroxidase, nadh oxidase and nadh peroxidase in the microaerophilic protozoan parasite giardia duodenalis. a cytosolic h2o-producing nadh oxidase and membrane-associated nadh peroxidase were readily detected from g. duodenalis. in all giardia strains investigated the nadh oxidase was present in high levels (1.2-2 u (mg protein)-1). using the same technique, nadh oxidase activity was also detected in the ... | 1995 | 8538699 |
| zoonotic intestinal parasites of hamadryas baboons papio hamadryas in the western and northern regions of saudi arabia. | six hundred and thirty-three faecal samples were randomly collected and examined for ova and cysts of intestinal parasites from five groups of hamadryas baboons of different population densities, with different human contact and in different ecological conditions (al-baha, turabah and al-taif in south-western and al-rihat and al-akhal in north-western saudi arabia). nine parasites were recorded (giardia lamblia, entamoeba histolytica, escherichia coli, balantidium coli, enterobius sp., trichuris ... | 1995 | 8544227 |
| carbohydrate epitopes of entamoeba histolytica cell surface glycoproteins are major targets of the human humoral response. | the antigens of entamoeba histolytica recognized by antibodies in 11 individual sera from patients treated for amebic liver abscess were determined both by immunoprecipitation of metabolically-radiolabeled whole trophozoite proteins and by immunoblotting.collectively, twenty-s even antigens ranging from 167 to 21 kda were detected in immunoblots of whole trophozoite extracts; eight of these were recognized by all tested patient sera. immunoprecipitation studies also revealed a complex amebic ant ... | 1995 | 8546039 |
| the seroprevalence of ten zoonoses in two villages of crete, greece. | the seroprevalence and incidence of 10 zoonoses due to rickettsia typhi, r. conorii, coxiella burnetii, burcella sp., borrelia sp., toxoplasma sp., leishmania sp., entamoeba histolytica, echinococcus granulosus and fasciola hepatica were studied in an animal husbandry and a farming village in crete, greece. the serum conversion incidence of each infectious agent was determined by testing 2 blood samples, collected in 1985 and in 1987. the surveillance was conducted using detailed transparent map ... | 1995 | 8549708 |
| the relationship of water sources and other determinants to prevalence of intestinal protozoal infections in a rural community of saudi arabia. | a study was carried out in a rural area of asir region in southwest of saudi arabia where information on the types of domestic water supply and sociodemography in relation to intestinal protozoal infections were obtained from 1417 inhabitants (81.4% of the total) of nine villages. stool samples were collected from the study population and examined for intestinal parasites. overall, the prevalence rates of infection with the common water-borne parasites, giardia lamblia and entamoeba histolytica ... | 1995 | 8550870 |
| the association of the amoebic colitis and chronic ulcerative colitis. | three patients with idiopathic ulcerative colitis and infection of entamoeba histolytica were reviewed. they represented three different relationships between the two conditions, vis asymptomatic carrier, coexisting infection and superinfection causing exacerbation of colitis. a two-way relationship between amoebic colitis and ulcerative colitis has been discussed and the possible mechanisms in the literature have been reviewed. in patients with a short history of diarrhoea and in tropical count ... | 1995 | 8553098 |
| entamoeba histolytica axenic growth improvement by ox bile. | axenic entamoeba histolytica cultures, grown in pehps medium, showed increasing yields and growth velocity when added with 0.03 to 0.6 g/l gallbladder ox bile, while higher doses were toxic for amebas. the highest density (505 +/- 29 x 10(3) trophozoites/ml) and shortest duplication time (dt = 10.74 +/- 0.2 h) corresponded to cultures added with 0.24 g bile/l. their yields were 1.74 and 3.34 times higher, respectively, than those which were reached by the non-added pehps controls and by growth o ... | 1995 | 8555742 |
| prevalence of antibodies against entamoeba histolytica in mexico measured by elisa. | the prevalence of antibodies against entamoeba histolytica was studied in the mexican population using an immunoenzyme assay in solid phase (elisa) and semiautomatic equipment. the antigen was a mixture of membrane proteins obtained by triton x-100 extraction from an axenic culture of entamoeba histolytica hm1-imss. the method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. from the 60,538 samples supplied by the national seroepidemiology ... | 1995 | 8557086 |
| purification and characterization of the acetate forming enzyme, acetyl-coa synthetase (adp-forming) from the amitochondriate protist, giardia lamblia. | giardia lamblia, an amitochondriate eukaryote, contains acetyl-coa synthetase (adp-forming), an enzyme known only from one other eukaryote (entamoeba histolytica) and a few anaerobic prokaryotes. the enzyme has been purified about 350-fold. the activity in the direction of acetate formation was dependent on adp and inorganic phosphate. the reverse reaction could not be detected. succinyl-coa, propionyl-coa and dadp were utilized with lower efficiency. the enzyme did not utilize amp plus ppi thus ... | 1996 | 8557109 |
| spontaneous release of cysteine proteinases but not of pore-forming peptides by viable entamoeba histolytica. | invasive properties of pathogenic entamoeba histolytica have been postulated to depend on the secretion or release of cysteine proteinases and pore-forming peptides (amoebapores) by trophozoites. to establish whether such toxic molecules are released by viable trophozoites or upon cellular disintegration, amoebae were maintained in various culture media, and activities in supernatants were monitored over time in correlation to cellular integrity. by measuring the release of the cytoplasmic marke ... | 1995 | 8559589 |
| activity of a new oxadiazole compound, against experimental infections with entamoeba histolytica and giardia lamblia in animal models. | bti 2286e(+/-)-e-3-(4-methylsulphinylstyryl)-1,2,4-oxadiazole has demonstrated potent amoebicidal activity in a single-dose treatment against entamoeba histolytica infection in the livers of golden hamsters and the caeca of mice, hamsters and rats. it has intra luminal activity against entamoeba criceti, a natural infection in golden hamster, and anti-giardial activity against giardia lamblia infection in suckling mice. bti 2286e is more potent than metronidazole in extra intestinal, intra lumin ... | 1993 | 8561524 |
| entamoeba histolytica encodes a highly divergent beta-tubulin. | the microtubules of the amitochondrial parasite entamoeba histolytica are atypical in certain respects. consistent with this, we report that e. histolytica encodes the most divergent beta-tubulin identified to date, with only 54% to 58% identity to beta-tubulins from various species. a similarly divergent beta-tubulin is encoded by the related entamoeba invadens; single gene copies appear to be present in both organisms. the entamoeba sequences were compared with a database of 101 beta-tubulins, ... | 1996 | 8563707 |
| evaluation of an enzyme-linked immunoassay for the detection in serum of antibodies to entamoeba histolytica. | a commercially available enzyme-linked immunoassay (elisa) was compared to an indirect hemagglutination test (iha) for the detection of antibodies to entamoeba histolytica on 225 patients' serums. all of the 107 serums that had iha titers of < 32 (interpreted as excluding the presence of invasive amebiasis) were elisa negative. sixty-three of 68 (93%) serums that had iha titers of > or = 128 (interpreted as indicative of the presence of active or recent infection) were elisa positive. fifty seru ... | 1995 | 8565416 |
| isolation and axenization of two entamoeba histolytica strains. | we describe the isolation and axenization of two e. histolytica strains, obtained from the stools of two patients with the clinical diagnosis of dysentery. we used pavlova's medium for initial polixenic culture, and tyi-s-33 (diamond's) medium for monoxenic and axenic cultures. in order to eliminate the microorganism contaminating the stools the following antibiotics were used: penicillin, streptomycin, ampicillin, chloramphenicol, kanamycin, nistatin, ceftriaxone and amphoterycin b. both strain ... | 1995 | 8566669 |
| rapid diagnosis of entamoeba infection by using entamoeba and entamoeba histolytica stool antigen detection kits. | humans are infected by two morphologically identical species of entamoeba: entamoeba histolytica causes amebic colitis and liver abscess, and entamoeba dispar is noninvasive. several weeks of culture and isoenzyme (zymodeme) analysis are required to differentiate e. histolytica from e. dispar. here we report a field trial of commercial antigen detection kits designed to rapidly detect and differentiate e. histolytica from e. dispar in stool specimens. stool specimens from 202 patients with diarr ... | 1995 | 8567882 |
| prevalence of enteric parasitic infection among hiv-infected attendees of an inner city aids clinic. | one hundred individuals confirmed to be infected with the human immunodeficiency virus (hiv), volunteered to participate in a pilot study comparing clinical status and enteric parasite burden with race, age, cd4 levels, risk factors and sexual practice. the prevalence of enteric parasites was 55 (55%) for giardia lamblia, 6 (6%) for cryptosporidium, 10 (10%) for isospora belli and 3 (3%) for entamoeba histolytica. there was no associations between demographic variables and the presence of parasi ... | 1995 | 8574144 |
| molecular analysis of two hexokinase isoenzymes from entamoeba histolytica. | the zymodemes, electrophoretic patterns of hexokinase, phosphoglucomutase and glucose phosphate isomerase isoenzymes, have been widely used to determine the pathogenicity of entamoeba histolytica isolates. although pathogenic and nonpathogenic forms of e. histolytica differ clearly in sequences of many homologous genes, a conversion between pathogenic and nonpathogenic zymodemes has been reported by several laboratories. to approach the question what might be the basis for the observed conversio ... | 1995 | 8577326 |
| identification and analysis of the start site of ribosomal rna transcription of entamoeba histolytica. | in this article we report the identification of the start site of ribosomal rna transcription unit of the enteric parasite e. histolytica. we cloned the upstream region of the ribosomal rna and we defined the 5' boundary of the transcription unit with nuclear run-on assays. we report that ribosomal transcription starts 2447 bp upstream the ssu ribosomal gene, at an adenosine residue. this data was supported both by s1 mapping and by primer extension analysis; that the mapped site was indeed the ... | 1995 | 8577327 |
| transcription of protein-coding genes in entamoeba histolytica is insensitive to high concentrations of alpha-amanitin. | 1995 | 8577336 | |
| kinetic mechanism of pyrophosphate-dependent phosphofructokinase from giardia lamblia. | the steady-state kinetics of the reaction catalyzed by inorganic-pyrophosphate-dependent d-fructose-6-phosphate 1-phosphotransferase from giardia lamblia have been investigated. the reactants for the forward and reverse reactions were the mg-chelated complexes of pyrophosphate (ppi) and pi. uncomplexed ligands were not substrates. in the direction of phosphorylation of fructose-6-phosphate (f6p), initial velocity double-reciprocal plots for both ppi and f6p were intersecting suggesting sequentia ... | 1995 | 8577346 |
| 'the tropics in our bathroom': chronic diarrhoea after return from the tropics. | interest in imported tropical diseases has increased with the rising number of travellers to the tropics. this is especially true in the case of tropical gastroenterologic disorders. the causative organisms of chronic diarrhoea are different from those causing acute diarrhoea. bacteria are relatively unusual; parasites, e.g. entamoeba histolytica or giardia lamblia or an opportunistic parasitic infestation associated with an hiv infection are more likely. furthermore, non-infectious causes, such ... | 1995 | 8578232 |
| intestinal opportunistic parasites among different groups of immunocompromised hosts. | the present study was designed to find out the prevalence of different intestinal opportunistic parasites in different groups of immunocompromised hosts; patients suffering from malignancy with or without immuno-suppressive drugs, or with chronic renal failure, or diabetes mellitus, or subjects under cortisone therapy. examination of stool samples collected from 427 immunocompromised hosts revealed the detection of intestinal opportunistic parasites in 98 samples, with a prevalence of 23%. infec ... | 1995 | 8586866 |
| chronic diarrhea among adults in kigali, rwanda: association with bacterial enteropathogens, rectocolonic inflammation, and human immunodeficiency virus infection. | one hundred patients with chronic diarrhea were seen in the department of internal medicine at the centre hospitalier de kigali, rwanda; stool and/or rectal swab culture was performed for these patients, and they underwent rectoscopy and serological testing for human immunodeficiency virus type 1 (hiv-1). enteropathogenic bacteria were isolated from 39 (39%) of the patients: shigella species (22 of 100 patients tested), non-typhi salmonella (11/100), aeromonas species (5/60), and campylobacter s ... | 1995 | 8589155 |
| [interleukin-8 gene expression in the human colon epithelial cell line, ht-29, exposed to entamoeba histolytica]. | the protozoan parasite, entamoeba histolytica, is one of major causative agents of intestinal disease all over the world. in acute experimental infection, the early host response to e. histolytica is characterized by an infiltration of neutrophils. however, the chemotactic signal for this response is not well known. based on the finding that human epithelial cells produce the potent neutrophil chemoattractant and activator, interleukin-8 (il-8), il-8 gene expression was examined thoroughly in hu ... | 1995 | 8591014 |
| axenization of entamoeba histolytica, a korean strain ys-27. | in the present communication, ys-27, a korean strain of entamoeba histolytica is described for the isolation and establishment of axenic cultivation. e. histolytica, designated as strain "ys-27", was isolated from the pus of a hepatic abscess obtained from a 72 year old inpatient of august 10, 1969. specimens, were obtained by needle aspiration, inoculated immediately and weekly cultured in a modified diphasic medium at 37 degrees c. strain ys-27 had been maintained for more than 15 years by wee ... | 1995 | 8591018 |
| [parasitic diseases of the liver]. | the liver seems to be very attractive for many parasites which may either inhabit the organ or pass through during their normal development or may be carried to the liver leading to destruction there. malaria parasites have a short developmental stage in the hepatocytes, but they do not damage the organ. for the leishmanias (leishmania donovani complex) are the kupffer cells are the target cells causing visceral leishmaniasis or kala azar. nematodes of the ascarid group have a special affinity f ... | 1995 | 8600687 |
| oral immunization with a recombinant cysteine-rich section of the entamoeba histolytica galactose-inhibitable lectin elicits an intestinal secretory immunoglobulin a response that has in vitro adherence inhibition activity. | the lc3-encoded 52-kda recombinant protein includes amino acids 758 to 1134 of the 170-kda subunit of the galactose-inhibitable lectin. oral immunization of balb/c mice with the lc3-encoded protein and cholera holotoxin induced an intestinal secretory immunoglobulin a (iga) response (p < 0.01 compared with the control). there was a negative correlation (p = 0.001) between intestinal anti-lc3 iga and serum iga and igg antibody responses. intestinal secretions from immunized mice completely inhibi ... | 1996 | 8606122 |
| cloning and expression of an entamoeba histolytica napd+(-)dependent alcohol dehydrogenase gene. | in this paper we cloned, sequenced and expressed a novel entamoeba histolytica alcohol dehydrogenase gene (ehadh3). ehadh3 has a predicted 383 amino acids open reading frame, encoding for a 42.3 kda protein. the deduced amino acid sequence showed 24 to 26% identity to other type iii alcohol dehydrogenases found in prokaryotic and lower eukaryotic organisms, but not in mammalia. there are at least two ehadh3 gene copies in the genome, but only a 1.2 kb transcript was detected. the ehadh3 fusion p ... | 1996 | 8611619 |
| the outpatient management of bloody diarrhea in young children. | 1995 | 8613324 | |
| oral immunization with an attenuated vaccine strain of salmonella typhimurium expressing the serine-rich entamoeba histolytica protein induces an antiamebic immune response and protects gerbils from amebic liver abscess. | attenuated salmonellae represent attractive candidates for the delivery of foreign antigens by oral vaccination. in this report, we describe the high-level expression of a recombinant fusion protein containing the serine-rich entamoeba histolytica protein (srehp), a protective antigen derived from virulent amebae, and a bacterially derived maltose-binding protein (mbp) in an attenuated strain of salmonella typhimurium. mice and gerbils immunized with s. typhimurium expressing srehp-mbp produced ... | 1996 | 8613356 |
| fibronectin-induced intracellular calcium rise in entamoeba histolytica trophozoites: effect on adhesion and the actin cytoskeleton. | the interaction of entamoeba histolytica trophozoites with fibronectin (fn) promotes adhesion of the protein to the cells and its later degradation by locally released proteases. binding to fn-covered surfaces induces, in addition, the formation of actin adhesion plates and focal contacts in the amebas. the signaling mechanisms underlying the response to fn are incompletely understood. in this paper we examined the modifications of cytosolic free calcium ([ca2+]i) induced in the trophozoites by ... | 1996 | 8617326 |
| entamoeba histolytica: isoprenylation of p21ras and p21rap in vitro. | 1996 | 8617332 | |
| entamoeba histolytica: involvement of pp125fak in collagen-induced signal transduction. | the interaction of entamoeba histolytica trophozoites with collagen involves cell adherence, formation, and release of electron dense granules (edgs) containing collagenase activity leading to the degradation of the bound protein. the binding is thought to be mediated by an "integrin-like" collagen receptor. since the signal transduction mechanisms triggered by the collagen-trophozoite interaction are unknown, but clearly involve cytoskeletal organization, we decided to explore the role of prote ... | 1996 | 8617343 |
| entamoeba histolytica: localization of a 30-kda cysteine proteinase using a monoclonal antibody. | we produced a monoclonal antibody against a major cysteine proteinase of 30kda from trophozoites of entamoeba histolytica strain hm1:imss. the specificity of the monoclonal antibody was confirmed by specific inhibition of azocasein digestion and by electrophoretic analysis, in the presence of sodium dodecyl sulfate or on a substrate gel, of the antigen precipitated by the antibody. immunofluorescent staining of trophozoites with the monoclonal antibody revealed heterogeneity in the intensity of ... | 1996 | 8617344 |
| identification of the galactose-adherence lectin epitopes of entamoeba histolytica that stimulate tumor necrosis factor-alpha production by macrophages. | the 170-kda subunit of the galactose-adherence lectin (gal-lectin) of entamoeba histolytica mediates adherence to human colonic mucins and intestinal epithelium as a prerequisite to amebic invasion. the gal-lectin is an immunodominant molecule and a protective antigen in the gerbil model of amebiasis. tumor necrosis factor alpha (tnf-alpha) produced by activated macrophages enhances nitric oxide-dependent cytotoxicity in host defense against e. histolytica. the purpose of this study was to ident ... | 1995 | 8618866 |
| the effect of chronic intestinal parasitic infection on maternal and perinatal outcome. | the main objective of this prospective cohort study was to investigate the prevalence, risk factors and maternal-perinatal consequences of chronic asymptomatic intestinal parasitic infection during pregnancy. | 1996 | 8620997 |
| [a case of amoebiasis as a latent infection]. | a 69-year-old male was admitted because of constipation and abdominal distension. a colon fiberscopic examination revealed the formation of ulceration. the trophozite of entamoeba histolytica was detected from from the specimens of colon mucosal biopsy of the ulcer. the stool was positive for the cysts of e. histolytica too. he had been in china and southeast asia during world war ii. he returned home and experienced amoebic dysentry, and was treated with emethin hydrochlolide. he suffered from ... | 1996 | 8621966 |
| prevalence of enteropathogens in stools of rural maasai children under five years of age in the maasailand region of the kenyan rift valley. | stool samples were collected during august 1994 from seventy rural maasai children under the age of five years who were living in the maasailand region of the kenyan rift valley. microbiological analysis was carried out on these samples to identify which intestinal pathogens were present among the infant population of the maasai. of the samples studied 54% were pathogen positive. the most common pathogen isolated was giardia lamblia which was detected in 31% of the samples. other pathogens that ... | 1996 | 8625866 |
| evidence for the existence of both proteasomes and a novel high molecular weight peptidase in entamoeba histolytica. | to screen for high molecular weight proteases in entamoeba histolytica, we subjected a soluble amebal extract to density gradient centrifugation and tested the fractions for activity against the chymotryptic peptide substrate, suc-leucyl-leucyl-valyl-tyrosyl-4-methylcoumaryl-7-amide. two peaks of activity, of approximately 11 and 20 s, were clearly separated. based on sds-electrophoretic pattern and immunoblot analysis, we ascribe the 20 s activity to proteasomes. the 11 s protein was purified f ... | 1996 | 8626412 |
| replication initiates at multiple dispersed sites in the ribosomal dna plasmid of the protozoan parasite entamoeba histolytica. | in the protozoan parasite entamoeba histolytica (which causes amoebiasis in humans), the rrna genes (rdna) in the nucleus are carried on an extrachromosomal circular plasmid. for strain hm-1:imss, the size of the rdna plasmid is 24.5 kb, and 200 copies per genome are present. each circle contains two rrna transcription units as inverted repeats separated by upstream and downstream spacers. we have studied the replication of this molecule by neutral/neutral two-dimensional gel electrophoresis and ... | 1996 | 8628298 |
| shortened amoebapore analogs with enhanced antibacterial and cytolytic activity. | amoebapores are cytolytic peptides of entamoeba histolytica which function by the formation of ion channels in target cell membranes. three isoforms (amoebapore a, b, and c) exist in amoebic cytoplasmic granules. they are composed of 77 amino acid residues arranged in four alpha-helical domains. in order to analyze the structure-function relationships, 15 synthetic peptides of 24-25 residues were constructed based on the assumption that the third helix is the membrane-penetrating domain and on t ... | 1996 | 8641476 |
| unbiased estimation of symmetrical directional mutation pressure from protein-coding dna. | the most generally applicable procedure for obtaining estimates of the symmetrical, or strandnonspecific, directional mutation pressure (microd) on protein-coding dna sequences is to determine the g+c content at synonymous codon sites (psyn), and to divide psyn by twice the arithmetic mean of the g+c content at synonymous codon sites of a large number of randomly generated, synonymously coding dna sequences (psyn). unfortunately, the original procedure yields biased estimates of psyn and microd ... | 1996 | 8642618 |
| intestinal protozoa. | giardia is the best known cause of protozoal gastrointestinal disease in north america, producing significant but not life-threatening gastrointestinal distress and diarrhea. although diagnosis of giardiasis may be challenging, treatment is usually successful. entamoeba histolytica poses a rarer but far more difficult clinical challenge. dysentery caused by e. histolytica may be the most feared intestinal protozoal infection, although cryptosporidium parvum, balantidium coli, isospora belli, sar ... | 1996 | 8644565 |
| pyrophosphate-dependent phosphofructokinase of entamoeba histolytica: molecular cloning, recombinant expression and inhibition by pyrophosphate analogues. | by using oligonucleotide primers derived from regions highly conserved in prokaryotic and eukaryotic phosphofructokinase sequences, a genomic dna fragment was amplified and used to isolate cdna and genomic clones coding for ppi-dependent phosphofructokinase (ppi-pfk) of entamoeba histolytica. the open reading frame consists of 1308 bp and the corresponding protein has a calculated molecular mass of 47.6 kda. the n-terminal half of the protein shows 27-35% identity with ppi-pfks or atp-dependent ... | 1996 | 8645233 |
| [low digestive hemorrhage caused by amebic colitis]. | amebiasis is an infectious disease produced by entamoeba histolytica, which has invasion capacity of the colon mucosa. it has different clinical forms, varying from the asymptomatic carrier state to severe, although not frequent, fulminant or necrotizing colitis, with an important necrosis of the colon mucosa. perforation or intestinal bleeding are possible. we report one case of patient who had a history of recent travel to india. was admitted with a clinical picture of abdominal pain, diarrhea ... | 1996 | 8645519 |
| humoral and mucosal iga antibody response to a recombinant 52-kda cysteine-rich portion of the entamoeba histolytica galactose-inhibitable lectin correlates with detection of native 170-kda lectin antigen in serum of patients with amebic colitis. | humoral and mucosal iga responses to a recombinant cysteine-rich portion (designated lc3) of the entamoeba histolytica galactose-inhibitable lectin's 170-kda subunit were determined in patients with amebic colitis. all patients had 170-kda amebic antigen in serum, compared with 1 of 50 cyst passers and 1 of 31 controls (p < .01). seven days after treatment, serum and fecal 170-kda antigen became undetectable in 12 of the 13 patients (p < .001). serum anti-lc3 iga was found in 83.8% of colitis pa ... | 1996 | 8655985 |
| diagnosis of amoebic infection of the liver: report of 36 cases. | the classical clinical picture of amoebic infection of the liver consists of fever, right upper quadrant pain and hepatomegaly. in recent years, the widespread availability of ultrasound and serology made an early diagnosis possible, which could result in less prominent clinical pictures. thirty six cases of liver amoebiasis diagnosed in antwerp between 1985 and 1992, were reviewed. three patients acquired their infection in belgium. for the other patients, the average delay between arrival in b ... | 1995 | 8669977 |
| characterization of adhesion plates induced by the interaction of entamoeba histolytica trophozoites with fibronectin. | entamoeba histolytica trophozoites are pleiomorphic and highly motile cells. although scarce fibrous material can be identified in the cytoplasm as elements of an organized cytoskeleton, clearly defined actin-containing structures are formed at the site of cell-matrix contact upon the interaction of trophozoites with fibronectin (fn) and other cellular matrix substrates. the structures are reminiscent of the adhesion plaques or focal contacts found in higher eukaryotic cells, where actin filamen ... | 1995 | 8674132 |
| [the role of aminoglycosides in hiv-infected patients]. | the frequency of serious infections due to pseudomonas aeruginosa is increasing among patients with advanced hiv infection; most of the cases are community-acquired. early diagnosis and adequate treatment--usually with the combination of an anti-pseudomonas beta-lactam antibiotic and an aminoglycoside--have a crucial bearing on prognosis. the good in vitro activity of aminoglycosides against mycobacteria contrasts with the need for intravenous administration and toxicity. in patients with mycoba ... | 1996 | 8677419 |
| protozoan infections in the male genital tract. | we reviewed the literature on protozoan infections in the male genital tract, with special reference to histopathological findings and their repercussions on different andrological functions. | 1996 | 8683676 |
| amebiasis and the entamoeba histolytica gal/galnac lectin: from lab bench to bedside. | 1996 | 8689398 | |
| antiamoebic activity of piper longum fruits against entamoeba histolytica in vitro and in vivo. | the fruits of piper longum used in traditional remedies against intestinal distress have been tested for their efficacy against experimental caecal amoebiasis of rats. the ethanolic extract, hexane fraction, n-butanol soluble fraction exerted in vitro amoebicidal action at 1000 micrograms/ml and the chloroform fraction at 500 micrograms/ml. the ethanolic extract and piperine, a pure compound, from this plant material cured 90% and 40% of rats with caecal amoebiasis, respectively. | 1996 | 8691851 |
| complementation of an escherichia coli adhe mutant by the entamoeba histolytica ehadh2 gene provides a method for the identification of new antiamebic drugs. | the pathogenic protozoan parasite entamoeba histolytica, the cause of amebic dysentery and amebic liver abscess, is an obligate anaerobe, and derives energy from the fermentation of glucose to ethanol with pyruvate and acetyl coenzyme a as intermediates. we have isolated ehadh2, a key enzyme in this pathway, that is a nad+- and fe2+-dependent bifunctional enzyme with acetaldehyde dehydrogenase and alcohol dehydrogenase activities. ehadh2 is the only known eukaryotic member of a newly defined fam ... | 1996 | 8692838 |
| the influence of conserved aromatic residues on the electron transfer reactivity of 2[4fe-4s] ferredoxins. | the detailed mechanism used by [4fe-4s] ferredoxins to exchange electrons is not known. the importance of two highly conserved aromatic residues, each located close to one cluster of 2[4fe-4s] ferredoxins has been probed by site-directed mutagenesis of clostridium pasteurianum ferredoxin. all generated variants are less stable than the native protein and only hydrophobic residues can replace one of the two conserved aromatic residues. with leucine substituting both aromatics, clostridium pasteur ... | 1996 | 8695647 |
| cloning and expression of a putative alcohol dehydrogenase gene of entamoeba histolytica and its application to immunological examination. | to clone and express the genes encoding major antigens of entamoeba histolytica, we constructed a lambda gt11 cdna library for e. histolytica hm1:imss and screened it with pooled sera from patients with amoebiasis. a 1,223-bp cdna was cloned (clone 1223), and its nucleotide sequence was determined. the amino acid sequence predicted to be encoded by the open reading frame of clone 1223 consisted of 396 residues and showed 32.5 and 32.3% homology to the nadh-dependent butanol dehydrogenases i and ... | 1996 | 8705667 |
| [application of the polymerase chain reaction to the epidemiological study of amoebiasis]. | the polymerase chain reaction (pcr) has been used in studying the pathogenicity of entamoeba histolytica in a rural community in shandong province. formalin-fixed stool samples were used for extraction of dna. the pcr amplifications were performed using two sets of primer that discriminate between pathogenic and nonpathogenic e. histolytica. all of the 32 cases of cyst carrier diagnosed microscopically were identified as positive. three groups were defined through pcr analysis: 6 cases (18.8%) w ... | 1995 | 8706101 |
| transient expression of luciferase in entamoeba histolytica driven by the ferredoxin gene 5' and 3' regions. | we have successfully transfected entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. we have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. our optimal construct gave values of 268 x 10(3) relative light units per second (rlu s-1) when assayed representing a stimulation of 18,000-fold over the control vector. comparison of differing constructs allowed us to conclud ... | 1995 | 8719240 |
| pcr-based quantitation of transfected luciferase dna: evaluation using an entamoeba histolytica reporter construct. | 1996 | 8723913 | |
| role of signalling and cytoskeletal rearrangements in the pathogenesis of entamoeba histolytica. | virulence of entamoeba histolytica is characterized by intestinal tissue invasion, engulfment of host cells and the formation of liver abscesses. the actin-rich cytoskeleton of the amoeba allows rapid changes in morphology in response to signals from external stimuli. cellular and molecular studies have described some of the proteins that participate in signalling and in cytoskeletal changes. | 1996 | 8727599 |
| phylogenetic analysis of beta-tubulin sequences from amitochondrial protozoa. | it has been proposed that certain extant anaerobic protozoa are descended from organisms that diverged early in eukaryotic evolution prior to the acquisition of mitochondria. among these are the extracellular parasites giardia lamblia, trichomonas vaginalis, and entamoeba histolytica, and the obligately intracellular microsporidia. phylogenetic analysis of rrna sequences from these amitochondrial organisms suggests that g. lamblia, t. vaginalis, and microsporidia are near the base of the eukaryo ... | 1996 | 8728394 |
| intestinal parasites of humans in a highland community of irian jaya, indonesia. | an indigenous, highland community in the oksibil valley of irian jaya, indonesia, was examined on two occasions to determine the prevalence of intestinal parasites. overall, 478 subjects from three villages in the study area, about 10% of the available population, were sampled. using a standard, wet-mount technique, 15 different species of parasites were found. hookworm was predominant in all age groups, with prevalences of 51%-86%, followed by ascaris lumbricoides (46%-57%) and trichuris trichi ... | 1996 | 8729627 |