Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| trichomoniasis. | trichomoniasis is perhaps the most common curable sexually transmitted disease worldwide, yet few resources are devoted to its control. it is associated with potentially serious complications such as preterm birth and human immunodeficiency virus acquisition and transmission. the immunology of a related organism, tritrichomonas foetus, which causes disease in cattle, has been investigated to some extent, but more work is needed for the human strain, trichomonas vaginalis. in addition, although t ... | 2004 | 15489349 |
| infections of the lower female genital tract during childhood and adolescence. | to review the pathogenesis, clinical presentation, diagnosis and treatment of lower female genital tract infections at a young age. | 2004 | 15491059 |
| targeted screening for trichomonas vaginalis with culture using a two-step method in women presenting for std evaluation. | trichomonas vaginalis is the most common nonviral sexually transmitted pathogen. wet mount microscopy performs poorly compared with culture; however, universal screening using culture would be cost-prohibitive. | 2004 | 15502673 |
| reproductive tract infections in rural women from the highlands, jungle, and coastal regions of peru. | to define the prevalences and manifestations of reproductive tract infections (rtis) in rural peruvian women. | 2004 | 15508193 |
| non-mitochondrial complex i proteins in a hydrogenosomal oxidoreductase complex. | trichomonas vaginalis is a unicellular microaerophilic eukaryote that lacks mitochondria yet contains an alternative organelle, the hydrogenosome, involved in pyruvate metabolism. pathways between the two organelles differ substantially: in hydrogenosomes, pyruvate oxidation is catalysed by pyruvate:ferredoxin oxidoreductase (pfor), with electrons donated to an [fe]-hydrogenase which produces hydrogen. atp is generated exclusively by substrate-level phosphorylation in hydrogenosomes, as opposed ... | 2004 | 15510149 |
| trichomonads under microscopy. | trichomonads are flagellate protists, and among them trichomonas vaginalis and tritrichomonas foetus are the most studied because they are parasites of the urogenital tract of humans and cattle, respectively. microscopy provides new insights into the cell biology and morphology of these parasites, and thus allows better understanding of the main aspects of their physiology. here, we review the ultrastructure of t. foetus and t. vaginalis, stressing the participation of the axostyle in the proces ... | 2004 | 15525428 |
| trichomonas vaginalis inhibits proinflammatory cytokine production in macrophages by suppressing nf-kappab activation. | activation of nf-kappab leads to the production of proinflammatory cytokines such as il-12 and tnf-alpha that are involved in innate and adaptive immunity. we determined whether t. vaginalis-induced inflammatory response in macrophages associated with nf-kappab. t. vaginalis adhesion led to transient nf-kappab activation at 6 h but activation declined dramatically by 8 h. super-shift assays showed that the gel-shifted complexes consisted of p65 (rel a) and p50 (nf-kappab1). nf-kappab activation ... | 2004 | 15528993 |
| evaluation of a cognitive-behavioral, group, randomized controlled intervention trial to prevent sexually transmitted infections and unintended pregnancies in young women. | few cognitive-behavioral interventions have focused on preventing sexually transmitted infections (stis) and unintended pregnancies (ups) in young, sexually active women in a single study. military recruit training provides a well-defined, national, nonclinic sample in which to evaluate such an intervention. | 2005 | 15530594 |
| chronic prostatitis: charlottesville to seattle. | since few men with chronic prostatitis/chronic pelvic pain syndrome (cp/cpps) have culturable bacteria by traditional approaches, we used sensitive molecular methods to determine presence of fastidious microorganisms. | 2004 | 15538207 |
| barriers to infectious disease care among lesbians. | despite the considerable number of women in the united states who identify as lesbian, few data exist that address lesbians' health needs. the institute of medicine emphasized that data on sexually transmitted infections, pap smear screening, and cervical dysplasia among lesbians were needed to guide clinical practice, policy development, and patient education. use of surveillance data for this purpose is limited because risk classifications exclude same-gender sex among women or subsume it unde ... | 2004 | 15550210 |
| health risk factors among detained adolescent females. | to identify the prevalence of health risk factors among a sample of detained adolescent females and determine whether there are racial/ethnic differences. | 2004 | 15556741 |
| comparative in vitro cytotoxic effects of ornidazole, metronidazole and ciprofloxacin against trichomonas vaginalis trophozoites. | a strain of trichomonas vaginalis, isolated from a patient complaining of vaginal discharge, was incubated and cultivated to compare the in vitro effects of ornidazole, metronidazole and ciprofloxacin on t. vaginalis trophozoites in terms of minimal inhibition concentrations (mics) and minimal lethal concentrations (mlcs). mic levels at 24 hours for ornidazole, metronidazole and ciprofloxacin were 50 mg/ml, 50 mg/ml, and 750 mg/ml. corresponding mlc levels were the same. in this in vitro study, ... | 2004 | 15565912 |
| local antigen in serodiagnosis of trichomoniasis vaginalis. | 2004 | 15573226 | |
| trichomonas hydrogenosomes contain the nadh dehydrogenase module of mitochondrial complex i. | hydrogenosomes are double-membraned atp-producing and hydrogen-producing organelles of diverse anaerobic eukaryotes. in some versions of endosymbiotic theory they are suggested to be homologues of mitochondria, but alternative views suggest they arose from an anaerobic bacterium that was distinct from the mitochondrial endosymbiont. here we show that the 51-kda and 24-kda subunits of the nadh dehydrogenase module in complex i, the first step in the mitochondrial respiratory chain, are active in ... | 2004 | 15577909 |
| growth patterns and antigenic analysis of egyptian trichomonas vaginalis isolates. | the vaginal washouts from symptomatic and asymptomatic patients were examined by wet mount examination and culture on modified tym medium. among the 320 cases examined, 10 were positive for t. vaginalis trophozoites by wet mount examination and culture. modified tym medium proved very satisfactory for isolation as well as maintenance of the 10 t. vaginalis isolates. comparison between the growth patterns of all isolates, by counting the number of viable organisms every 24 hours for 7 days, showe ... | 2004 | 15587311 |
| cryopreservation of trichomonas vaginalis: a trial of using four different cryoprotectants. | to reach the maximum survival rate of trichomonas vaginalis after cryopreservation, four substances: dimethylsulphoxide (dmso), glycerol, egg yolk and horse serum were used as cryoprotectants. t. vaginalis trophozoites, in the logarithmic phase, cultured on tym-s medium were used. rapid and slow freezing schedules were followed. examinations for viable organisms were done after a month duration in liquid nitrogen. immediate and delayed thawing were tried. viability scores depended on the presenc ... | 2004 | 15587319 |
| biological variability of trichomonas vaginalis clinical isolates from symptomatic and asymptomatic patients. | the vaginal specimens of sixty women were cultured using modified thioglycolate medium. thirteen samples were positive for trichomonas vaginalis 21.7%. pathogenicity of these clinical isolates was investigated by intra-peritoneal injection to mice. additionally, their susceptibility to metronidazole was evaluated. all the clinical isolates produced infection in mice but marked variations in pathogenicity and susceptibility were observed. four out of 13 isolates (30.8%) produced mortality in mice ... | 2004 | 15587322 |
| prevalence and determinants of genital shedding of herpes simplex virus among women attending italian colposcopy clinics. | to determine the prevalence of herpes simplex virus (hsv) shedding among women attending italian colposcopy clinics and describe their lifestyle, demographic characteristics, genital symptoms and signs. | 2005 | 15596279 |
| [research progress on the detection methods of trichomoniasis vaginalis]. | 2004 | 15597718 | |
| sexually transmitted infections among brothel-based sex workers in bangladesh: high prevalence of asymptomatic infection. | objective and goal: the goal of this study was to study the prevalence of sexually transmitted infections (stis)/reproductive tract infections (rtis) among brothel-based sex workers (sws) in bangladesh. | 2005 | 15614116 |
| neisseria gonorrhoea, chlamydia trachomatis, and treponema pallidum infection in antenatal and gynecological patients at korle-bu teaching hospital, ghana. | five hundred and seventeen women attending the gynecology and obstetrics clinics of the korle-bu teaching hospital were examined for sexually transmitted infections (stis). vaginal swabs were examined for trichomonas vaginalis, candida albicans, and gardnerella vaginalis infection. endocervical swabs were examined for neisseria gonorrhoea and chlamydia trachomatis using a recently developed rna detection kit. strain typing was performed to identify serovars of c. trachomatis. sera were analyzed ... | 2004 | 15623949 |
| contribution of hiv-1 infection to acquisition of sexually transmitted disease: a 10-year prospective study. | sexually transmitted diseases (stds) enhance human immunodeficiency virus (hiv)-1 susceptibility, but few studies have examined the reciprocal effect of hiv-1 on std acquisition. | 2004 | 15633091 |
| [clinical relevance of antibiotic resistance in obstetrics and gynecology]. | antibiotic resistance of microorganisms that cause infections of the urogenital tract is a clinically relevant problem in obstetrics and gynecology. due to methodological difficulties, resistance testing is rarely used for the management of these infections. therefore, solid epidemiological data on resistance rates of most involved pathogens are scarce. antibiotic resistance of several microorganisms appears to be increasing in various areas of the world, mainly trichomonas vaginalis and gardner ... | 2005 | 15644637 |
| aetiological agents of vaginitis in nigerian women. | this study focuses on the identification of aetiological agents of vaginitis in nigerian women. study subjects are drawn from patients presenting with lower abdominal pain, vaginal discharge and itching at the gynaecology clinic of lagos university teaching hospital and at the clinical centre of the nigerian institute of medical research, yaba, lagos, between january 2001 and july 2002. a total of 250 patients gave informed consent to participate in the study. the patients also had pre- and post ... | 2004 | 15649008 |
| sexually transmitted infections and increased risk of co-infection with human immunodeficiency virus. | the incidence of trichomoniasis (trichomonas vaginalis) in the united states is estimated at 5 million cases annually; chlamydia (chlamydia trachomatis) at 3 million; gonorrhea (neisseria gonorrhoeae), 650,000; and syphilis (treponema pallidum), 70,000. however, most sexually transmitted infections (stis) are asymptomatic-contributing to underdiagnosis estimated at 50% or more. diagnosis of an sti signals sexual health risk because an sti facilitates the transmission and acquisition of other sti ... | 2004 | 15653780 |
| a trichomonas vaginalis 120 kda protein with identity to hydrogenosome pyruvate:ferredoxin oxidoreductase is a surface adhesin induced by iron. | trichomonas vaginalis, a human sexually transmitted protozoan, relies on adherence to the vaginal epithelium for colonization and maintenance of infection in the host. thus, adherence molecules play a fundamental role in the trichomonal infection. here, we show the identification and characterization of a 120 kda surface glycoprotein (ap120) induced by iron, which participates in cytoadherence. ap120 is synthesized by the parasite when grown in 250 microm iron medium. antibodies to ap120 and the ... | 2005 | 15659068 |
| long-term survival and intracellular replication of mycoplasma hominis in trichomonas vaginalis cells: potential role of the protozoon in transmitting bacterial infection. | the existence of a symbiotic relationship between trichomonas vaginalis and mycoplasma hominis, which is the first reported example of symbiosis between two obligate human pathogens, has been recently reported by our research group. in this work, we examined the cellular location of m. hominis in respect to t. vaginalis. by using gentamicin protection assays, double immunofluorescence, and confocal microscopy, we obtained strong evidence that m. hominis is located within protozoan cells. 5-bromo ... | 2005 | 15664961 |
| the appropriateness of core group interventions using presumptive periodic treatment among rural zimbabwean women who exchange sex for gifts or money. | to map the characteristics of rural based sex workers in zimbabwe with regard to demographics, mobility, behavior, hiv and sexually transmitted infection (sti) prevalence, to explore the appropriateness and feasibility of presumptive periodic treatment (ppt) for bacterial stis as an hiv prevention intervention among these women, and to compare tolerability of 2 ppt regimens (1 g of azithromycin and 2 g of metronidazole+/-500 mg of ciprofloxacin). five commercial farms and 2 mines in mashonaland ... | 2005 | 15671806 |
| synthesis and antiparasitic properties of new 4-n-benzylamino-4-hetarylbut-1-enes. | new derivatives of 4-n-benzylamino-4-hetarylbut-1-ene containing a pyridyl nucleus were synthesized from benzylamines and pyridine aldehydes. n-oxide derivatives were obtained from these homoallylamines. study of the antiparasitic properties of obtained pyridine derivatives as well as of four related benzazepines previously described, was carried out using cytotoxicity assays against trichomonas vaginalis and epimastigote form of trypanosoma cruzi protozoa. compounds showing activity against epi ... | 2005 | 15674802 |
| screening injecting drug users for sexually transmitted infections and blood borne viruses using street outreach and self collected sampling. | injecting drug users (idus) are a marginalised population, who infrequently access health services for screening for sexually transmitted infections (stis) and blood borne viruses (bbvs), and are at high risk of these infections. | 2005 | 15681724 |
| mycoplasma genitalium: an organism commonly associated with cervicitis among west african sex workers. | to identify the contribution of mycoplasma genitalium to the aetiology of cervicitis in sub-saharan africa and its relative importance in the overall burden of sexually transmitted infections among female sex workers (fsw). | 2005 | 15681727 |
| [emergency care for women following sexual assault: characteristics of women and six-month post-aggression follow-up]. | this study evaluated the process and results of treatment for women at a university hospital after sexual violence. a prospective study of 166 women (> or = 12 years of age) treated from october 1999 to february 2002 included six months follow-up after aggression. half of the women were under 20 years of age, two were illiterate, 70.0% unmarried, 20.0% used contraceptives, and 80.0% received treatment within the first 24 hours post-aggression. nearly 80.0% of aggressors were unknown to victims a ... | 2005 | 15692652 |
| use of an immunochromatographic assay for rapid detection of trichomonas vaginalis in vaginal specimens. | trichomonas vaginalis infection is estimated to be the most widely prevalent nonviral sexually transmitted infection in the world. wet-mount microscopy is the most common diagnostic method, although it is less sensitive than culture. the osom trichomonas rapid test (genzyme diagnostics, cambridge, mass.) (referred to here as osom) is a new point-of-care diagnostic assay for t. vaginalis that uses an immunochromatographic capillary flow (dipstick) assay and provides results in 10 min. the purpose ... | 2005 | 15695664 |
| [analysis on the relative factors of trichomonal vaginitis in married childbearing age women in rural impoverished area]. | to investigate the status and relative factors of trichomonal vaginitis among married childbearing age women in rural impoverished area of the northwestern part of china and hence identify the risk factors and provide a basis for the prevention and treatment of trichomonal vaginitis. | 2005 | 15702794 |
| [study of acute vulvovaginitis in sexually active adult women, with special reference to candidosis, in patients of the francisco j. muñiz infectious diseases hospital]. | the results of microbiological vaginal secretions samples obtained from 749 women (from july 2001 to july 2002) were studied in the bacteriology unit of the francisco javier muñiz hospital from buenos aires. all patients suffered acute vulvovaginitis were child bearing and sexually active women, 334 of them were hiv-positive. the following are the results of the microbiological studies: lactobacillus spp 50.6%, gardnerella vaginalis 25.6%, candida spp 17.4%, trichomonas vaginalis 5.3%, neisseria ... | 2004 | 15709796 |
| sexually transmitted infections in rural madagascar at an early stage of the hiv epidemic: a 6-month community-based follow-up study. | sexually transmitted infections (stis) in madagascar have primarily been monitored in selected groups of patients attending sti clinics in major cities as part of the hiv surveillance program in madagascar. | 2005 | 15729151 |
| the genome of the protist parasite entamoeba histolytica. | entamoeba histolytica is an intestinal parasite and the causative agent of amoebiasis, which is a significant source of morbidity and mortality in developing countries. here we present the genome of e. histolytica, which reveals a variety of metabolic adaptations shared with two other amitochondrial protist pathogens: giardia lamblia and trichomonas vaginalis. these adaptations include reduction or elimination of most mitochondrial metabolic pathways and the use of oxidative stress enzymes gener ... | 2005 | 15729342 |
| experience with routine vaginal ph testing in a family practice setting. | despite recommendations by centers for disease control and the american college of obstetricians and gynecologists, ph testing is infrequently performed during the evaluation of vaginitis. consequently, little information exists on its use in a primary care setting. | 2004 | 15739819 |
| evolutionary biology: the hydrogenosome's murky past. | 2005 | 15744282 | |
| effects of bacterial vaginosis and other genital infections on the natural history of human papillomavirus infection in hiv-1-infected and high-risk hiv-1-uninfected women. | whether the natural history of human papillomavirus (hpv) infection is affected by bacterial vaginosis (bv) or trichomonas vaginalis (tv) infection has not been adequately investigated in prospective studies. | 2005 | 15747249 |
| heterologous expression in tritrichomonas foetus of functional trichomonas vaginalis ap65 adhesin. | trichomonosis, caused by trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. the interaction of t. vaginalis with vaginal epithelial cells (vecs), a step preparatory to infection, is mediated in part by the prominent surface protein ap65. the bovine trichomonad, tritrichomonas foetus, adheres poorly to human vecs. thus, we established a transfection system for heterologous expression of the t. vagin ... | 2005 | 15748280 |
| [frequency of vaginal infections in pregnant women in the department of perinatology and gynaecology in zabrze]. | the analysis of vaginal infections in pregnant women admitted to the department of perinatology and gynaecology in zabrze, from january 2001 to september 2003. | 2004 | 15751213 |
| spliceosomal introns in the deep-branching eukaryote trichomonas vaginalis. | eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. nuclear premrna splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. here we demonstrate the presence of a splicing apparatus in the protist trichomonas vaginalis and show that rna motifs found in yeast and metazoan introns are required for splicing. we also describe the first introns in this deep-br ... | 2005 | 15764705 |
| is the presence of trichomonas vaginalis a reliable predictor of coinfection with chlamydia trachomatis and/or neisseria gonorrhoeae in female ed patients? | the aim of the study was to determine if the presence of trichomonas vaginalis (tv) is associated with an increased risk of coinfection with chlamydia trachomatis (ct) and/or neisseria gonorrhoeae (ng) in female patients presenting to the ed with symptoms consistent with a sexually transmitted disease (std). | 2005 | 15765329 |
| prevalence of candida albicans and trichomonas vaginalis in pregnant women in havana city by an immunologic latex agglutination test. | we aimed to estimate the prevalence of candida albicans and trichomonas vaginalis in immunocompetent pregnant women living in havana city, cuba, with or without symptoms of vaginitis, using a sample of 640 women from 6 gyneco-obstetrics hospitals, which represents 2.5% of total yearly pregnant women. diagnosis was made using a new latex agglutination kit (newvagin c-kure, la habana, cuba). clinical sensitivity and specificity of this assay were validated against culture method, with 467 and 489 ... | 2004 | 15775877 |
| nematocidal and trichomonacidal activities of 2-substituted quinolines. | several quinolines were synthesized and evaluated in vitro and in vivo against the nematodes caenorhabditis elegans, heligmosomoides polygyrus and the protozoa trichomonas vaginalis. if some of them have shown in vitro nematocide activity (at 10 microm), however, their trichomonacidal activity reached 50% reduction at only 100 microm. the in vivo activity on trichinella spiralis model was evaluated for some of the most in vitro active quinolines. | 2005 | 15784240 |
| vaginal swabs versus lavage for detection of trichomonas vaginalis and bacterial vaginosis among hiv-positive women. | cervicovaginal lavage (cvl) is often used for research and may be easier and more accurate than vaginal swabs as a specimen collection method. | 2005 | 15788920 |
| the prevalence of chlamydia, gonorrhea, and trichomonas in sexual partnerships: implications for partner notification and treatment. | treatment of sex partners by patient-delivered partner therapy (pdpt) may prove to be an effective strategy in reducing reinfection and preventing the sequelae of sexually transmitted infections (stis). however, limited data exists regarding stis within sexual partnerships (dyads). | 2005 | 15788928 |
| clinically resistant trichomoniasis. | 2005 | 15800103 | |
| collecting the essence of man: semen collection for hiv transmission studies in sub-saharan africa. | 2005 | 15800108 | |
| antibiotics for bacterial vaginosis or trichomonas vaginalis in pregnancy: a systematic review. | to determine whether antibiotic treatment for bacterial vaginosis or trichomonas vaginalis during pregnancy decreases the risk of preterm birth and associated adverse outcomes. | 2005 | 15802417 |
| sexually transmitted infections among pregnant women attending antenatal clinics in mongolia: potential impact on the mongolian hiv epidemic. | we conducted mongolia's first nation-wide cross-sectional survey of sexually transmitted infections (stis) among pregnant women attending prenatal care. among our 2000 participants, 386 (19.3%) were infected with chlamydia trachomatis, 133 (6.7%) with trichomonas vaginalis, 121 (6.1%) with neisseria gonorrhoeae, and 128 (6.4%) were seropositive for treponemal antibodies. none of our participants were seropositive for hiv infection. additionally, 605 (30.3%) of the women had at least one sti, 133 ... | 2005 | 15807944 |
| synthesis and biological properties of new 5-nitroindazole derivatives. | a series of new 3-alkoxy- or 3-hydroxy-1-[omega-(dialkylamino)alkyl]-5-nitroindazoles have been synthesized and their trichomonacidal, antichagasic and antineoplastic properties studied. five derivatives (5, 6, 8, 9 and 17) showed remarkable trichomonacidal activity against trichomonas vaginalis at 10 microg/ml concentration. three compounds (8, 10, 11) exhibited interesting antichagasic activity and these same compounds moderate antineoplastic activity against tk-10 and ht-29 cell lines. unspec ... | 2005 | 15809155 |
| structural implications of novel diversity in eucaryal rnase p rna. | previous eucaryotic rnase p rna secondary structural models have been based on limited diversity, representing only two of the approximately 30 phylogenetic kingdoms of the domain eucarya. to elucidate a more generally applicable structure, we used biochemical, bioinformatic, and molecular approaches to obtain rnase p rna sequences from diverse organisms including representatives of six additional kingdoms of eucaryotes. novel sequences were from acanthamoeba (acathamoeba castellanii, balamuthia ... | 2005 | 15811915 |
| seroepidemiology of trichomonas vaginalis in rural women in zimbabwe and patterns of association with hiv infection. | serological assays using dried blood spots from 5221 women in rural areas of eastern zimbabwe were used to assess the epidemiology of trichomonas vaginalis infection, and its association with hiv. antibodies to t. vaginalis and to hiv were detected by enzyme immunoassays. behavioural and demographic data were collected by confidential questionnaires. in total, 516 (9.9%) women were seropositive for t. vaginalis and seroprevalence increased with age among younger women. divorced, widowed and sing ... | 2005 | 15816157 |
| identification of a subversive substrate of trichomonas vaginalis purine nucleoside phosphorylase and the crystal structure of the enzyme-substrate complex. | trichomonas vaginalis is an anaerobic protozoan parasite that causes trichomoniasis, a common sexually transmitted disease with worldwide impact. one of the pivotal enzymes in its purine salvage pathway, purine nucleoside phosphorylase (pnp), shows physical properties and substrate specificities similar to those of the high molecular mass bacterial pnps but differing from those of human pnp. while carrying out studies to identify inhibitors of t. vaginalis pnp (tvpnp), we discovered that the non ... | 2005 | 15817485 |
| effect of piroxicam, metamizol, and s-adenosylmethionine in a murine model of experimental trichomoniasis. | biological effects of piroxicam, metamizol, and s-adenosylmethionine (s-amet) have been tested in nmri mice infected intraperitoneally with trichomonas vaginalis. an intraperitoneal treatment during ten preinfection days with piroxicam (10 mg/kg/day), or metamizol (275 mg/kg/day), but not with s-amet (117 mg/kg/day) induced a significant decrease of abdominal lesions and mortality, assessed by means of a pathogenicity index. the trichomonicidal activity of piroxicam, metamizol, and s-amet was te ... | 2005 | 15828587 |
| the diagnosis of trichomonas vaginalis in liquid-based pap tests: morphological characteristics. | we investigated the morphological features of trichomonas vaginalis in liquid-based papanicolaou (pap) (lbp) tests, in order to assess the impact of this new slide preparation system on recognition of t. vaginalis organisms. we reviewed 88 lbp test slides previously interpreted as showing t. vaginalis and assessed morphological characteristics of the organisms and the presence of secondary features. eighty-six of 88 slides showed, at least focally, organisms with cytoplasmic granules, nuclei, an ... | 2005 | 15830365 |
| in vitro resistance of neisseria gonorrhoeae to metronidazole. | susceptibility of 64 clinical isolates of neisseria gonorrhoeae to metronidazole was determined by the agar dilution technique using mueller-hinton and chocolate agar media. all isolates were resistant to metronidazole at 12.5 mug/ml in either medium. this lack of activity of metronidazole against n. gonorrhoeae would suggest that the ability to recover n. gonorrhoeae in patients being treated with metronidazole for concurrent trichomonas vaginalis infections should not be hampered. | 1974 | 15830482 |
| [study on surface adhesion protein 33 gene sequence of different trichomonas vaginalis isolates]. | to study genetic polymorphism of surface adhesion protein 33 (ap33) gene on the seven isolates of trichomonas vaginalis. | 2004 | 15830862 |
| molecular cloning and characterization of a rac1 homologue cdna from trichomonas vaginalis. | to clone and characterize a rac1 homologue from trichomonas vaginalis for studying cell cycle of the organism. | 2004 | 15830884 |
| [routine examination of leukorrhea in 3347 patients with trichomonal vaginitis]. | 2004 | 15830896 | |
| increased prevalence of leukocytes and elevated cytokine levels in semen from schistosoma haematobium-infected individuals. | in this study, we investigated the seminal inflammatory response to egg infestation of the urogenital organs in 240 semen-donating men aged 15-49 years living in a schistosoma haematobium-endemic area of madagascar. in 29 subjects (12%) with excretion of > or =5 ova/ejaculate, leukocytospermia (>10(6) leukocytes/ml) and the presence of seminal lymphocytes and eosinophil leukocytes were each significantly more prevalent than in 74 subjects (31%) who were s. haematobium negative (p<.01). in additi ... | 2005 | 15838790 |
| clinical presentation and management of alleged sexually assaulted females at mulago hospital, kampala, uganda. | to determine the presentation and treatment offered to sexually assaulted females attending emergency gynaecological ward in mulago hospital, kampala, uganda. | 2005 | 15843131 |
| identification of trichomonas vaginalis cysteine proteases that induce apoptosis in human vaginal epithelial cells. | a secreted cysteine protease (cp) fraction from trichomonas vaginalis is shown here to induce apoptosis in human vaginal epithelial cells (hvec) and is analyzed by mass spectrometry. the trichomonad parasite t. vaginalis causes one of the most common non-viral sexually transmitted infection in humans, trichomoniasis. the parasite as well as a secreted cysteine protease (cp) fraction, isolated by affinity chromatography followed by bio-gel p-60 column chromatography, are shown to induce hvec apop ... | 2005 | 15843376 |
| trichomonas vaginalis polyamine metabolism is linked to host cell adherence and cytotoxicity. | trichomonas vaginalis secretes putrescine that is readily detected in vaginal secretions. we wanted to examine the effect of decreased putrescine synthesis by inhibition of ornithine decarboxylase (odc) on t. vaginalis. one reason is because inhibition of tritrichomonas foetus odc results in growth arrest, destruction of hydrogenosomes, and decreased amounts of hydrogenosomal enzymes. treatment of t. vaginalis t016 with >/=20 mm 1,4-diamino-2-butanone (dab) to inhibit odc resulted in growth arre ... | 2005 | 15845462 |
| is a change in the vaginal flora associated with an increased risk of preterm birth? | the purpose of this study was to determine if a change in the vaginal flora was associated with an increased risk of preterm birth, and to determine if metronidazole therapy before 32 weeks increased the risk of preterm birth. | 2005 | 15846235 |
| [application of an alternative method to counting in neubauer chamber to determine trichomonas vaginalis concentrations]. | it was demonstrated that there is a relation of cell concentration with the parasite reading in an elisa reader. it was determined that absorbance showed significant values in wavelengths of visible range and the possible minimum wavelength (450 nm)was chosen to guarantee the maximum sensitivity. likewise, it was confirmed that absorbance significantly increased (p < 0.001) after the plate was filled up, it stabilized in the 40m-2h time interval when applying 100, 150, 200 or 300 ml per well of ... | 2001 | 15846921 |
| synthesis and antiprotozoan evaluation of new alkyl-linked bis(2-thioxo-[1,3,5]thiadiazinan-3-yl) carboxylic acids. | two new series of several alkyl-linked bis(2-thioxo-[1,3,5]thiadiazinan-3-yl) carboxylic acids were synthesized in a two step procedure from the corresponding alkyl bis-dithiocarbamic salt intermediary. the novel compounds were evaluated for their activity in vitro against trypanosoma cruzi strain cl (clone cl b5) and trichomonas vaginalis strain jh 31a. | 2005 | 15848753 |
| [behavior of vaginal trichomoniasis in a group of adolescents]. | 275 adolescents aged 10-19 receiving attention at the infantojuvenile department of pregnancy interruption and of sexually transmitted infections of "ramón gonzalez coro" and "eusebio hernández" gynecoobstetric teaching hospitals, both in havana city, were studied from august, 1999, to september, 2000, aimed at knowing the behavior of the infection caused by trichomonas vaginalis in this population group. the results yielded 18.1 % of positivity to parasitosis. it was proved by the calculation o ... | 2003 | 15849923 |
| [frequency of trichomonas vaginalis infection in couples with fertility problems]. | one-hundred and seventy two couples which went for the first time to the infertility service of the national institute of endocrinology from june 1999 to june 2000, were studied to find out the frequency of trichomonas vaginalis infection in this group, and determine its interaction with a number of clinical and risk variables. the results yielded that 10.5% were positive to the parasite, the prevailing symptom was leukorrea in women and 96.6% of men showed no symptoms. it was highly significant ... | 2002 | 15849932 |
| rifaximin, a peculiar rifamycin derivative: established and potential clinical use outside the gastrointestinal tract. | rifaximin is a poorly absorbed semisynthetic rifamycin derivative with a broad spectrum of antibacterial activity including gram-positive and gram-negative bacteria, both aerobes and anaerobes. although originally developed for the treatment of infectious diarrhea, the appreciation of the pathogenic role of gut bacteria in several organic and functional gastrointestinal diseases has increasingly broadened its clinical use. the availability of a topical formulation (a cream containing 5% of the d ... | 2005 | 15855757 |
| trichomonas vaginalis: identification of soluble and membrane-associated phospholipase a1 and a2 activities with direct and indirect hemolytic effects. | a direct hemolytic activity, dependent on phospholipase a (pla) activity, was located in the particulate subcellular fraction (p30) of trichomonas vaginalis. we identified soluble direct and indirect hemolytic activities in the spent medium and soluble fraction (s30) of t. vaginalis strain gt-13. spent medium showed the highest specific indirect hemolytic activity (siha) at ph 6.0 (91 indirect hemolytic units [hu]/mg/hr). spent medium and p30, but not s30, showed direct hemolytic activity. pla a ... | 2005 | 15856864 |
| measuring the significance of field validation in the college of american pathologists interlaboratory comparison program in cervicovaginal cytology: how good are the experts? | expert opinion is often used as a gold standard for gynecologic cytology in the evaluation of new technologies, in the legal setting, and in the validation of cases for use in educational programs and proficiency testing. however, the reliability of expert opinion alone in selecting slides of a specific cytodiagnosis that can be reproducibly and reliably identified by subsequent reviewers has not been determined. | 2005 | 15859630 |
| increased sensitivity of trichomonas vaginalis isolation from vaginal secretions by subsequent blind passage of preliminary negative cultures. | trichomonas vaginalis is a vaginal protozoon causing the most prevalent nonviral sexually transmitted disease. culture is considered as the most reliable and gold standard among conventional diagnostic methods in the detection of t. vaginalis. in the present study, the diagnostic effect of subsequent blind culturing of vaginal secretion samples in diamond's complete medium at every 48 h was investigated in 93 women. vaginal secretion samples were examined by wet mount and inoculated into diamond ... | 2005 | 15878448 |
| the diagnosis of trichomonas vaginalis in liquid-based pap tests: correlation with pcr. | the conventional papanicolaou smear (cps) is not considered accurate for the diagnosis of trichomonas vaginalis (t. vaginalis), and women noted to carry the organism on cps are recommended to undergo confirmatory testing. liquid-based preparations have been shown to facilitate the diagnosis of squamous lesions and may also facilitate the diagnosis of t. vaginalis. we used polymerase chain reaction (pcr) to investigate the accuracy of the diagnosis of t. vaginalis by the liquid-based pap test (lb ... | 2005 | 15880709 |
| bacterial vaginosis and intravaginal practices: association with hiv. | to determine the prevalence of bacterial vaginosis and to identify risk factors associated with this condition among urban women in harare, zimbabwe. | 2004 | 15881309 |
| trichomonas vaginalis adherence mediates differential gene expression in human vaginal epithelial cells. | trichomonas vaginalis, an ancient protist, colonizes the vaginal mucosa causing trichomonosis, a vaginitis that sometimes leads to severe health complications. preparatory to colonization of the vagina is the adhesion to vaginal epithelial cells (vecs) by trichomonads. we hypothesized that vecs alter the gene expression to form a complex signalling cascade in response to trichomonal adherence. in order to identify the genes that are upregulated, we constructed a subtraction cdna library after co ... | 2005 | 15888089 |
| trichomonas vaginalis: intrastrain polymorphisms within the ribosomal intergenic spacer do not correlate with clinical presentation. | trichomoniasis presents a broad spectrum of clinical patterns ranging from asymptomatic to severe vaginitis and cervicitis. despite its importance, very little is known about the genetic relatedness of its causative agent, trichomonas vaginalis, and the clinical phenotypes. to address this question, analysis of restriction length polymorphism (rflp) within the intergenic spacer of the ribosomal dna (igs) from 60 clinically defined isolates of t. vaginalis was performed. this is the first descrip ... | 2005 | 15888292 |
| n-acetyl-beta-d-hexosaminidase from trichomonas vaginalis: substrate specificity and activity of inhibitors. | among chitinolytic activities previously described in trichomonas vaginalis, n-acetyl-beta-d-hexosaminidase (nahase) was the enzyme system expressing the highest level of specific activity. we report here some biochemical characteristics of nahase purified from t. vaginalis. we found at first that the use of 4-methylumbellifferyl-substrate was responsible for a substrate affinity for the enzyme, about 1000-fold higher than those when using p-nitrophenyl-substrates (pnp). whereas the optimum ph w ... | 2005 | 15890491 |
| treatment failure of vaginal trichomoniasis in clinical practice. | we have conducted a retrospective study of treatment failure of trichomonas vaginalis (tv) infection in a busy, urban genitourinary medicine clinic in birmingham, uk, between 1998 and 2002. the prevalence of non-response to standard doses of metronidazole without any history of reinfection or nonadherence was 1.7% (28/1603) overall, and has significantly increased from 0.38% in 1999 to 3.5% in 2002 (p = 0.001, chi2 test). there were no associated demographic factors. the majority of patients (53 ... | 2005 | 15899079 |
| unexpected diversity and differential success of dna transposons in four species of entamoeba protozoans. | we report the first comprehensive analysis of transposable element content in the compact genomes (approximately 20 mb) of four species of entamoeba unicellular protozoans for which draft sequences are now available. entamoeba histolytica and entamoeba dispar, two human parasites, have many retrotransposons, but few dna transposons. in contrast, the reptile parasite entamoeba invadens and the free-living entamoeba moshkovskii contain few long interspersed elements but harbor diverse and recently ... | 2005 | 15901838 |
| rubrerythrin and peroxiredoxin: two novel putative peroxidases in the hydrogenosomes of the microaerophilic protozoon trichomonas vaginalis. | the parasitic flagellate trichomonas vaginalis contains hydrogenosomes, anaerobic organelles related to mitochondria, that generate atp from the fermentative conversion of pyruvate to acetate, co2 and molecular hydrogen. although an essentially anaerobic organism, trichomonas encounters low oxygen concentrations in its natural habitat and has to protect itself, and especially the oxygen-sensitve enzymes of hydrogenosomal metabolism, from oxidative damage. we have identified two novel proteins in ... | 2005 | 15904985 |
| a high-throughput colorimetric and fluorometric microassay for the evaluation of nitroimidazole derivatives anti-trichomonas activity. | several nitroimidazole derivatives were synthesized and tested as possible trichomonicidal agents. a fast, simple, practical and reliable in vitro colorimetric method was applied to the screening of the nitroimidazole derivatives anti-trichomonas activity. the colorimetric technique was based on the use of alamar blue as a redox-indicator. the test was carried out both qualitatively (minimal inhibitory concentration determined by naked eye observation) and quantitatively (fluorometric determinat ... | 2005 | 15963680 |
| mucopurulent cervicitis: no longer ignored, but still misunderstood. | the last decade has produced considerable advances in the diagnosis of the common etiologies of mucopurulent cervicitis (mpc), including chlamydia trachomatis and neisseria gonorrhoeae, and in the delineation of key aspects of their pathogenesis. despite this, clear understanding of why these bacteria cause cervical inflammation in a minority of women who is infected with either organism is limited. furthermore, many women who have mpc have neither of these infections detected, even when highly ... | 2005 | 15963875 |
| cytologic findings in pap smears with actinomyces-like organisms. | to detect whether there is a relationship between the presence ofactinomyces-like organisms (alos) and cytologic findings. | 2005 | 15966286 |
| association between trichomoniasis and bacterial vaginosis: examination of 600 cervicovaginal smears. | to determine whether there is a relationship between trichomoniasis and bacterial vaginosis (bv), cervicovaginal smears obtained from 600 women were stained with the papanicolaou technique and examined cytologically. thirty-six (6%) of 600 women were diagnosed as having trichomonas vaginalis [tv(+)]. sixteen (44.4%) of 36 tv(+) cases were observed as having bv [bv(+)]. thirty-one of 564 tv(-) vaginal smears were also positive for bv (5.5%). there was a statistically significant correlation betwe ... | 2005 | 15969458 |
| trichomonas vaginalis-induced epithelial monolayer disruption and human immunodeficiency virus type 1 (hiv-1) replication: implications for the sexual transmission of hiv-1. | the objective of this study was to evaluate potential mechanisms of trichomonas vaginalis involvement in human immunodeficiency virus type 1 (hiv-1) transmission. polarized monolayer integrity of primary cervical and prostate epithelial cells or cell lines cultured with t. vaginalis was measured by monitoring transepithelium resistance. the effect of t. vaginalis isolates on hiv-1 passage through polarized epithelial cell monolayers was evaluated for hiv-1 p24gag in the basolateral supernatants. ... | 2005 | 15972505 |
| prevalence and correlates of chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis infections, and bacterial vaginosis among a cohort of young injection drug users in baltimore, maryland. | injection drug users (idus) consistently demonstrate high-risk behaviors for sexually transmitted infections (stis). this study examines sti prevalence and correlates among young idus. | 2005 | 15976603 |
| appearance of virus-like particles in tritrichomonas foetus after drug treatment. | tritrichomonas foetus is a parasitic protist that infects the urogenital tract of cattle causing bovine trichomonosis. virus-like particles (vlps) in protozoa have been reported in several parasites including trichomonas vaginalis, a human flagellate, but viruses were never described in t. foetus so far. herein we show for the first time the presence of vlps in t. foetus after several drug treatments. they were detected by electron microscopy and were confirmed by immunofluorescence microscopy u ... | 2005 | 15979116 |
| predicting adolescents' longitudinal risk for sexually transmitted infection: results from the national longitudinal study of adolescent health. | influencing adolescents' sexual behaviors has the potential to influence trajectories of risk for sexually transmitted infections (stis) among young adults. | 2005 | 15997000 |
| management of metronidazole-resistant trichomonas vaginalis--a new approach. | metronidazole-resistant trichomoniasis is an infrequent but challenging problem with no universally successful treatment. since 1994, we have been using a combination regimen consisting of high-dose tinidazole plus a broad-spectrum antibiotic, i.e. doxycycline or ampicillin, and clotrimazole pessaries, for 7--14 days in this condition. a retrospective case review identified 11 cases of resistant trichomonas between 1994 and 2002. in the absence of resistance testing, resistance was defined clini ... | 2005 | 16004628 |
| trichomonas vaginalis in a sheffield genitourinary medicine department. | the case-notes of all patients who were diagnosed with a first episode of trichomonas vaginalis (tv) between 1 october 2002 and 30 september 2003 were reviewed. a total of 78 patients were suitable for inclusion in the study. analysis of their notes revealed that, although the majority of patients presented with symptoms, 15% (n=12) of cases were asymptomatic. a raised vaginal ph was found in 94% (n=47) of the patients in whom it was measured. in all, 97% (n=76) of patients received treatment in ... | 2005 | 16004629 |
| recalcitrant trichomonas vaginalis infection--a case series. | the objective of this study was (1) to estimate the prevalence of recalcitrant trichomonas vaginalis (tv) infection in a uk genitourinary medicine clinic; (2) to use a case series and literature review to suggest an algorithm for management of recalcitrant tv (rtv). a retrospective review of laboratory records, case-notes and literature review was conducted. fifteen patients were studied, representing 1.16% of the cases presenting during the study period. a wide variety of therapeutic agents was ... | 2005 | 16004634 |
| a linear discrimination analysis based virtual screening of trichomonacidal lead-like compounds: outcomes of in silico studies supported by experimental results. | a computational (virtual) screening test to identify potential trichomonacidals has been developed. molecular structures of trichomonacidal and non-trichomonacidal drugs were represented using stochastic and non-stochastic atom-based quadratic indices and a linear discrimination analysis (lda) was trained to classify molecules regarding their antiprotozoan activity. validation tests revealed that our lda-qsar models recognize at least 88.24% of trichomonacidal lead-like compounds and suggest usi ... | 2005 | 16005626 |
| frequency of infectious agents for vaginitis in non- and hysterectomized women. | the frequency of infectious agents for vaginitis has shown varying results. bacterial vaginosis and candidiasis are infections related to vaginal ph alteration. vaginal ph is related to endocervical ph in pre- and post-menopaused women, and vaginal ph in hysterectomized women is more acidic than in non-hysterectomized women. the aim of this paper is to verify differences in papanicolaou smear diagnoses of infectious agents in hysterectomized women. a retrospective study was conducted at faculdad ... | 2005 | 16021494 |
| heterogeneity in extracellular nucleotide hydrolysis among clinical isolates of trichomonas vaginalis. | trichomonas vaginalis is a parasitic protozoan that causes trichomonosis, a sexually-transmitted disease, with serious sequelae to women and men. as the host-parasite relationship is complex, it is important to investigate biochemical aspects of the parasite that contribute to our understanding of trichomonal biology and pathogenesis. nucleoside triphosphate diphosphohydrolase 1 (ntpdase 1), which hydrolyses extracellular atp and adp, and ecto-5'-nucleotidase, which hyrolyses amp, have been char ... | 2005 | 16038398 |
| giardia mitosomes and trichomonad hydrogenosomes share a common mode of protein targeting. | mitochondria are archetypal organelles of endosymbiotic origin in eukaryotic cells. some unicellular eukaryotes (protists) were considered to be primarily amitochondrial organisms that diverged from the eukaryotic lineage before the acquisition of the premitochondrial endosymbiont, but their amitochondrial status was recently challenged by the discovery of mitochondria-like double membrane-bound organelles called mitosomes. here, we report that proteins targeted into mitosomes of giardia intesti ... | 2005 | 16040811 |
| [cloning and sequence analysis of trichomonas vaginalis ferredoxin gene]. | to construct a recombinant plasmid containing ferredoxin gene of trichomonas vaginalis. | 2005 | 16042202 |
| [genetic characteristics of trichomonas vaginalis]. | 2005 | 16042209 |