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[contact tracing in genital chlamydia infections].only one in five general practitioners in norway initiates notification of partner(s) after diagnosing a case of genital chlamydial infection. in order to increase the use of this efficient case-finding method we review the aims of notifying partners and the judicial, ethical and economical aspects of the method. we describe both provider-referral and patient-referral methods. the first approach is chosen when the index patient wants to remain anonymous. general practitioners are advised not to ...19921509433
ofloxacin versus doxycycline for treatment of cervical infection with chlamydia trachomatis.women with culture-proven chlamydia trachomatis cervical infection were randomized to receive either ofloxacin (300 mg) or doxycycline (100 mg), orally twice daily for 7 days. all 56 had negative cultures 5 to 9 days after treatment. four weeks after treatment, 26 (93%) of 28 ofloxacin-treated patients and all 22 doxycycline-treated patients were cured. we conclude that 300 mg of ofloxacin given twice daily for 7 days provides effective therapy for chlamydial infection of the cervix.19921510408
detection of chlamydia trachomatis by use of polymerase chain reaction.a polymerase chain reaction (pcr) assay was developed for detection of chlamydia trachomatis dna. one primer set was used, from the published sequence of the common c. trachomatis plasmid. detection of amplified sequences was carried out by agarose gel electrophoresis. analysis of 106 clinical samples tested by cell culture and pcr showed a sensitivity of 100% when pcr was compared with cell culture.19921513346
a woman with too much facial hair. evaluating the possibility of attenuated 21-hydroxylase deficiency in hirsutism. 20061513383
severity of illness and resource allocation in dnr patients in icu.this study examined the differences in severity of illness and resource allocation between patients with do-not-resuscitate (dnr) orders and non-dnr patients in intensive care units. severely ill intensive care patients with dnr orders continued receiving aggressive medical care following cardiac or respiratory arrest. the continued use of advanced therapies for dnr patients raises important questions about the use of costly intensive care resources.20061513394
lead screening and follow-up in an urban pediatric clinic.the purpose of this study was to examine screening and follow-up procedures used in testing for lead toxicity at an urban pediatric clinic. a retrospective chart review of all patients with elevated zinc protoporphyrin levels was undertaken. in the first four months of 1990, 66 of 438 patients screened (15.1%) had elevated zinc protoporphyrin levels. sixty charts were located and reviewed to evaluate five key interventional strategies. iron therapy was implemented in 31 (51.2%), and follow-up wa ...20061513512
location of the origin of replication for the 7.5-kb chlamydia trachomatis plasmid.the hypothetical origin of replication for the 7.5-kb plasmid common to chlamydia trachomatis is believed to be in a region of the plasmid that contains four 22-bp tandem repeats preceded by an a-t-rich region. to test this hypothesis, replication of plasmid dna in metabolically active reticulate bodies of the lymphogranuloma venereum biovar of c. trachomatis was examined by electron microscopy. the results presented show that the origin of replication appears to be near the tandem repeats of pc ...19921513879
d-penicillamine and prussian blue as antidotes against thallium intoxication in rats.d-penicillamine (dp) and prussian blue (pb), given alone and in combination, were evaluated in rats as treatments against acute thallotoxicosis. animals were poisoned by intraperitoneal (i.p.) injection of thallium(i) acetate at different doses (16, 30, 40, 50 and 70 mg/kg). later (24 h), treatments were administered until day 5, as follows: d-penicillamine (dp), 25 mg/kg, i.p. route, twice daily; prussian blue (pb), 50 mg/kg, oral route, twice daily. ld50 values were estimated for each treatmen ...19921514189
potential endocervical pathogens before termination. 19921515838
potential endocervical pathogens before termination. 19921515839
potential endocervical pathogens before termination. 19921515840
epidemiology and therapy of chlamydia trachomatis genital infection in women.out of 1172 females recruited in a screening program for genital infections, 144 (12.28%) were cervical and/or urethral positive for chlamydia trachomatis (ct) by enzyme immunoassay (eia). patients positive for ct showed no significant differences in terms of demography, sexual practices and clinical evidence as compared to a control group formed by ct-negative females randomly selected. historical data showed a higher frequency of previous pelvic inflammatory disease (pid) in patients as compar ...19921517809
recurrent genitourinary chlamydial infections in sexually active female adolescents.to determine the recurrence rate of chlamydial infections, we initially screened an urban population of 1308 sexually active female adolescents for chlamydial infection at the urethral and endocervical sites; these young women were followed and had additional examinations for infection. chlamydial infection was documented by tissue culture in 31.1% (407) of them at some time during the study. after appropriate antibiotic treatment, 68.3% (278/407) returned for test-of-cure cultures within 3 mont ...19921517932
alteration in t cell/macrophage ratio may reveal lymphocyte proliferation specific for the triggering antigen in reactive arthritis.it has previously been shown that synovial fluid (sf) mononuclear cells (mnc) from patients with reactive arthritis (rea) and some patients with undifferentiated oligoarthritis (uoa) respond specifically to the triggering bacterium (specific responders). however, in some patients there is a response to two or more bacteria (non-specific responders) and in a third group no response is found (non-responders). we assessed whether the proportion of synovial mnc which were macrophage-monocyte (mamo) ...19921519037
effectiveness and efficiency of selective vs universal screening for chlamydial infection in sexually active young women.since chlamydial cervicitis is not associated with specific complaints, screening asymptomatic women is an important initiative to prevent pelvic inflammatory disease and its sequelae. compared with universal screening, selective screening is less costly but less effective so the cost savings vs the consequences of missing infected women need to be weighed carefully.19921520050
chlamydia keratoconjunctivitis determination of chlamydia trachomatis specific secretory immunoglobulin a in tears by enzyme immunoassay.determination of chlamydia trachomatis specific antibodies in serum and tears, isolation of c. trachomatis in cell culture and c. trachomatis antigen detection by direct immunofluorescence test were evaluated for the laboratory diagnosis of chlamydia trachomatis infection in 30 patients with chronic conjunctivitis. c. trachomatis specific secretory immunoglobulin a (s-iga) was detected in the tears of all 8 patients with a positive result in culture and the direct immunofluorescence test. in 4 a ...19921521804
establishment and characterization of a sv40 t-antigen immortalized human bronchial epithelial cell line. 19921522038
prerequisite work-up of the couple before in-vitro fertilization.prerequisite examinations, including immune status for rubella and hepatitis b antigens, cultures for chlamydia trachomatis and mycoplasmas, pap smear and hystersalpingogram were performed in 227 couples before their enrollment into an in-vitro fertilization (ivf) programme. the examinations were completed in 187 couples. immune status for rubella had already been documented in 45% of the women: of the remainder, 11 patients were not immune (6%). a screening test for hepatitis b antigens had alr ...19921522189
male genital chlamydia trachomatis infections.the role of chlamydia trachomatis in infections of the male genital tract is reviewed. the organism is an important cause of non-gonococcal urethritis, post-gonococcal urethritis and epididymitis, but does not appear to play a major part in the pathogenesis of chronic abacterial prostatitis or in proctitis in anoreceptive homosexual men.19921522336
genital chlamydia trachomatis infections in the female. 19921522337
extra-genital chlamydia trachomatis infection as sexually-acquired reactive arthritis. 19921522338
advances in the antimicrobial therapy of chlamydial genital infections. 19921522339
laboratory methods for chlamydial infections. 19921522340
the laboratory diagnosis of male chlamydia trachomatis infections--a time for change?we carried out a two-phased study comparing the effectiveness of first-catch early morning urine (fcu) samples against urethral swabs for the detection of c. trachomatis in men. four hundred and seventeen new and re-booked consecutive men, who attended the department of genito-urinary medicine, bristol, having held their urine overnight, were recruited. patients who had received antimicrobial chemotherapy in the preceding 2 months were excluded. early morning fcu samples were obtained from 208 m ...19921522341
the differentiation of chlamydia species by antigen detection in sputum specimens from patients with community-acquired acute respiratory infections.an amplified enzyme immunoassay (ideia iii: dako diagnostics ltd) for detecting genus-specific chlamydia antigen was evaluated prospectively on 286 respiratory specimens from 275 patients presenting with community-acquired pneumonia or persistent chest infection. nineteen patients had evidence of recent chlamydial infection, having two or more positive sputum or serological markers. sputa from two other patients were elisa-positive in the absence of other positive criteria and were regarded as f ...19921522342
prevalence of antibodies to chlamydia pneumoniae in a belgian population.to evaluate the prevalence of antibodies to chlamydia pneumoniae in a healthy adult belgian population a study group of 150 medical students was chosen. sera were collected in the period between march and october 1990 and assessed by the microimmunofluorescence test. sixty-one per cent were found to have igg antibodies to c. pneumoniae in a titre greater than or equal to 16, which showed evidence of past infection. twenty-one per cent had iga in a titre greater than or equal to 8. in none were a ...19921522343
a comparison of the seroepidemiology of chlamydial infection in pigeon fanciers and farmers in the u.k.a detailed comparative seroepidemiological study of antibody responses was performed in 271 pigeon fanciers and 100 farmers. overall 73% of pigeon fanciers had igg antibodies at a titre greater than or equal to 16 to chlamydia pneumoniae, 39% to chlamydia psittaci, and 6.6% to chlamydia trachomatis. the prevalence of chlamydial antibodies was significantly lower in the farmers at 47% for c. pneumoniae, 6% for c. psittaci, and 2% for c. trachomatis. both populations were exposed to complex microb ...19921522344
[the philadelphia chromosome 30 years later].we review the changing concepts regarding the nature of the ph chromosome during the last thirty years. the role of molecular biology techniques in its identification are discussed as well as its present status in the diagnosis, prognosis and management of chronic myelogenous leukemia and acute lymphoblastic leukemia.20061523343
test-of-cure for asymptomatic genital chlamydial infections in women. a cost-benefit analysis.asymptomatic genital infection caused by chlamydia trachomatis is common, and one or more test-of-cure consultations in such cases is routine. the economic implications of two post-treatment strategies, either no test-of-cure, or one test-of-cure consultation with a single test for c. trachomatis, renewed treatment, and another test-of-cure of those still chlamydia-positive, and so on, have been compared. the costs of the test-of-cure strategy are twice those of the no-test regimen. without test ...19921523529
evaluation of a new chemiluminometric immunoassay, magic lite chlamydia, for detecting chlamydia trachomatis antigen from urogenital specimens.the chemiluminometric sandwich immunoassay, magic lite chlamydia by ciba corning (medfield, ma), using one chlamydia trachomatis-specific monoclonal antibody conjugated with acridinium ester and one polyclonal immobilized to magnetic particles, was compared to cell culture in 291 urogenital specimens from 92 men and 102 women tested both in the cervix and urethra. the proportion of patients with positive culture results ranged from 11.8% among the women to 15% among the men, using microculture p ...19921523532
a comparison of urine sample to urethral swab for detection of chlamydia trachomatis in asymptomatic young men using two enzyme immunoassays.first catch early morning urine samples were compared to urethral swabs for detection of chlamydia trachomatis in 405 male military conscripts, using 2 enzyme immunoassays, syva microtrak eia (sme) and abbott chlamydiazyme (ac). the prevalence rate of c. trachomatis was 7.9% (95% confidence limits 5.5%-11.0%), and the asymptomatic carrier rate was 7.8% (95% confidence limits 5.2%-11.2%). only 5 of 32 conscripts with positive test results for c. trachomatis complained of urethritis symptoms, and ...19921523533
[which women should be tested for chlamydia trachomatis in general practice?].specimens to test for chlamydia trachomatis and neisseria gonorrhoeae were collected as a routine from female patients undergoing gynecological examinations in general practice. in 3.9% of 517 non-pregnant women the culture for c trachomatis was positive. none were infected with n gonorrhoeae. c trachomatis was detected in ten of 138 (7.2%) women with genital symptoms, compared with ten of 379 (2.6%) without symptoms. the annual incidence rate for c trachomatis in asymptomatic patients was 0.3% ...19921523627
[chlamydia trachomatis and neisseria gonorrhoeae among women seeking abortion in norway. results from a nationwide study].in april and may 1991 all women requesting abortion in norway were screened for chlamydia trachomatis and neisseria gonorrhoeae. during the study period 2,194 abortions were carried out. the study included 2,110 women with representative tests for c trachomatis from the cervix uteri. only 1,702 women were tested for n gonorrhoeae. the prevalence of n gonorrhoeae was 0.5% (8:1,702), and of c trachomatis 5.4% (113:2,100). the prevalence of c trachomatis decreased from 9.1% among women less than or ...19921523628
[study of chlamydia trachomatis in chronic prostatitis].to assess the pathogenetic role of chlamydia trachomatis in non-bacterial prostatitis (nbp), aspiration biopsied specimens were examined for c. trachomatis by using in situ dna hybridization and antibody titer to c. trachomatis was measured. an enzyme-linked immunosorbent assay (elisa) for c. trachomatis specific iga was employed using purified c. trachomatis type l2 ebs. the positive rates of iga antibodies to c. trachomatis in serum, eps and vb3 were 25.6%, 31.5% and 29.4%, respectively. they ...19921523985
comparison of totally implanted and external catheters in paediatric oncology patients.from june 1982 until december 1989, 93 permanent central venous catheters [59 external catheters (ecs) and 34 implanted catheters (ics)] were placed in 69 patients. the median age of these patients at placement was 5.6 years for ecs and 8.8 years for ics (p less than 0.05). follow-up evaluation was possible on 86 catheters (58 ecs and 28 ics). the median time of insertion was 236 days and 316 days for ecs and ics, respectively (p less than 0.05). the median number of open days was 58 for ecs and ...20061524904
autoradiographic localization in polychaete embryos of tritiated mesulergine, a selective antagonist of serotonin receptors that inhibits early polychaete development.developing embryos of the polychaete ophryotrochal labronica were exposed to tritiated mesulergine, a selective antagonist of the serotonin receptors 5-ht1c and 5-ht2, that also has significant affinity to dopamine d-2 sites, and the labeling was analyzed by autoradiography. already at the earliest developmental stages (1-4 cells), numerous silver grains visualizing 3h-mesulergine binding sites and possibly also serotonin receptors were recorded over the cytoplasm, mostly in association with dec ...20061525017
relation between antibodies to chlamydia trachomatis and spontaneous abortion following in vitro fertilization.many couples undergo in vitro fertilization due to occlusion of the fallopian tubes. chlamydia trachomatis infections are a major cause of this tubal damage. since this organism has also been associated with poor pregnancy outcome, we investigated whether a past exposure to c. trachomatis was associated with spontaneous abortion following in vitro fertilization and embryo transfer.19921525448
the molecular biology and diagnostics of chlamydia trachomatis.the rapid development of biotechnological methods provides the potential of dissecting the molecular structure of microorganisms. in this review the molecular biology of chlamydia is described. the genus chlamydia contains three species c. trachomatis, c. psittaci, and c. pneumonia which all are important human pathogens. chlamydia is obligate intracellular bacteria with a unique biphasic life cycle. the extracellularly chlamydial elementary bodies (eb) are small, metabolic inactive, infectious ...19921526183
the value of non-culture techniques for diagnosis of chlamydia trachomatis infections: making the best of a bad job. 19921526232
accuracy of two enzyme immunoassays and cell culture in the detection of chlamydia trachomatis in low and high risk populations in senegal.two enzyme immunoassays (eias), chlamydiazyme (cz; abbott laboratories) and pathfinder (pf; kallestadt), were compared with a cell culture technique in the detection of cervical chlamydia trachomatis infection in 670 women in urban settings in senegal (377 pregnant women and 293 prostitutes). positive cz and positive pf specimens were tested a second time using a monoclonal antibody blocking technique. true positive specimens were defined as those positive on culture or positive on eia with conf ...19921526236
value of confirmation of chlamydiazyme enzyme immunoassay results in the detection of chlamydia trachomatis.the specificity of an eia (chlamydiazyme, abbott) for detection of chlamydia trachomatis was evaluated by means of a monoclonal antibody blocking reagent (abbott) and a direct fluorescent antibody test (dfa) (micro trak, syva). of the 12,864 tested specimens from the urethra and cervix, 666 (5.2%) were positive. when retested with the same technique 625 (4.9%) were again positive. the result in 514 (4.0%) specimens was verified by means of the blocking reagent. of specimens with absorbance value ...19921526241
evaluation of two rapid tests for the diagnosis of chlamydia trachomatis genital infections.the performance of two new enzyme immunoassays (eia) for the detection of chlamydia trachomatis in a practice setting was compared. a consecutive series of 207 female patients seen at an inner-city sexually transmitted disease clinic were tested by cell culture, the kodak surecell (sc) and abbott testpack chlamydia (tp) eias. in addition 210 male patients, selected by physicians on the basis of the fact that multiple urethral samples could be obtained, were tested by cell culture and sc. the pre ...19921526242
antimicrobial susceptibility and serotyping of chlamydia trachomatis strains isolated before and after treatment with ciprofloxacin and doxycycline. 19921526246
effects of vitamin a on retinal pigment epithelial cells in vitro.human retinal pigment epithelial (rpe) cell monolayers were used in an in vitro wound-healing experiment. wound closure were studied over time in the presence of various concentrations of all-trans retinol (vitamin a). it was found that 5-20 micrograms/ml (17.5-70 mumol/l) of vitamin a significantly inhibited wound closure by inhibiting cell migration and proliferation. in a collagen gel-contraction model, similar doses of vitamin a partially inhibited cell contractility. cellular morphology, as ...19921526732
bilateral occipital lobe infarction in acute migraine: clinical, neurophysiological, and neuroradiological study.a woman having common migraine attacks coincident with an asymmetrical bilateral occipital lobe infarction that spared the brainstem and cerebellum underwent these studies: serial electroencephalography, brainstem auditory, visual and somatosensory evoked potentials, magnetic resonance imaging of the brain and cerebral arteriography. the patient's vision improved greatly during a one-year follow-up. the absence of risk factors for stroke suggested that migraine caused the infarction in the poste ...20061526769
evaluation of the humoral immune response in trachoma to chlamydia trachomatis major outer membrane proteins by sequence-defined immunoassay.the chlamydia trachomatis immunodominant major outer membrane protein (momp) is both a target of neutralizing antibodies and the serotyping antigen and thus has been a focus of diagnostic, seroepidemiologic, and experimental investigations. the microimmunofluorescence (mif) test has been the principal tool in serologic investigations of chlamydial infections but is difficult and expensive for routine use; moreover, since it uses whole organisms as antigen, it is incapable of revealing the molecu ...19921527430
heterotypic protection of mice against chlamydial salpingitis and colonization of the lower genital tract with a human serovar f isolate of chlamydia trachomatis by prior immunization with recombinant serovar l1 major outer-membrane protein.intrauterine infection of mice with a human genital tract isolate of chlamydia trachomatis (serovar f) resulted in salpingitis. in some cases, oviduct damage was sufficient to cause infertility due to lumenal blockage. parenteral immunization with a purified, heterologous, recombinant major outer-membrane (rmomp) preparation reduced the proportion of animals developing severe salpingitis by 77% compared with mock-immunized controls, but failed to reduce chlamydial colonization of the lower genit ...19921527511
[chlamydia trachomatis antigens in endocervical samples and serum igg antibodies in sterile-infertile women using elisa].in this study, ch.trachomatis antigen was investigated by elisa, endocervical swab material of 35 infertile and sterile women, and 4 of them were antigen positive. all of these 4 cases were secondary sterile and 3 of them showed ch.trachomatis igg seropositivity. chlamydia igg seropositivity was seen in 8 of 35 cases. in this study, 68 patients were investigated for ch.trachomatis igg antibodies and 17 patients were found to be seropositive. these findings indicate the importance of ch.trachomat ...19921528141
detection of cervical chlamydia trachomatis and neisseria gonorrhoeae with deoxyribonucleic acid probe assays in obstetric patients.the gen-probe pace 2 deoxyribonucleic acid probe assays for the detection of chlamydia trachomatis and neisseria gonorrhoeae are targeted against the ribosomal ribonucleic acid of each pathogen. our study compared the performance of the probe assays with culture for chlamydia trachomatis (246 patients) and neisseria gonorrhoeae (310 patients) while screening obstetric patients.19921530008
microbial etiology of urban emergency department acute salpingitis: treatment with ofloxacin.we attempted to define the microbiologic characteristics of acute salpingitis in women presenting to an urban emergency department with pelvic inflammatory disease and to determine the effectiveness of ofloxacin in treating this disease.19921530018
monocyte disorders associated with t cell defects in patients with solid tumors.t cells proliferate in response to autologous monocytes in the autologous mixed lymphocyte reaction (amlr). amlr was found to be impaired in patients with advanced cancer (stages iii and iv), whereas normal values were found in the early stages of the disease (stages i and ii). peripheral t lymphocytes from patients with advanced stages also exhibited a decreased ability to produce interleukin-2 (il-2) during an amlr response, whereas production of il-2 by t cells in stages i and ii was comparab ...20061533752
laparoscopic cholecystectomy: an australian view. 20061534009
polymorphism in the rd (d6s45) gene.the rd (d6s45) gene in the class iii region of the hla major histocompatibility complex encodes a protein normally containing 24 consecutive basic-acidic dipeptide repeats. we determined the frequency of variations in the number of repeats by use of the polymerase chain reaction. of 107 subjects 7 (3.3%) carried genes encoding 22 or 23 repeats. there was no difference in the frequency of such polymorphisms between normal individuals and those with systemic lupus erythematosus, a disease associat ...19921534063
juvenile dermatomyositis.juvenile dermatomyositis is a relatively rare, multisystem disease characterized by a nonsuppurative myositis which causes symmetrical weakness, rash and vasculitis; this last can affect the gastrointestinal tract and the myocardium. late development of calcinosis is seen in approximately two thirds of patients. its etiology is unknown, although there are clues that it may be an unusual response to a viral infection. some 50% of children will have a very acute, rapidly progressive disease, while ...19931534378
[a case of fitz-hugh-curtis syndrome confirmed by laparoscopy].a 25-year-old japanese female was admitted to the department of surgery in kyushu koseinenkin hospital because of serious right hypochondralgia. gastrofiberscopy, abdominal ultrasonography, intravenous pyelography and irrigoscopy did not reveal the origin of the pain, and she was introduced to the department of internal medicine. because enzyme immunoassay of the uterine cervical specimen in the department of urology showed positive chlamydial antigen, we suspected her of perihepatitis induced b ...19921535331
restricted work-activity programs minimize injury compensation cost. 19911535427
use of an animal model for the study of the role of human immunodeficiency virus 1 in the human intestine.the gastrointestinal manifestations of human immunodeficiency virus (hiv)-1 disease have centered on identifiable causes of intestinal dysfunction such as parasitic and bacterial pathogens. the lamina propria of the intestine contains cell that harbor hiv-1, but the significance of this observation remains unknown. because limited animal models are available to evaluate the gastrointestinal effects of this infection, a system that uses human fetal intestine transplanted subcutaneously onto the b ...19921537521
detection of chlamydia pneumoniae by polymerase chain reaction.while criteria for serodiagnosis of chlamydia pneumoniae infection are well established, isolation of the organism is often difficult. to increase detection of this organism, c. pneumoniae-specific sequences were identified to permit amplification of c. pneumoniae by polymerase chain reaction (pcr). a cloned c. pneumoniae 474-bp psti fragment was shown by dot blot and southern hybridization to differentiate c. pneumoniae from the other chlamydia spp., react with all c. pneumoniae isolates tested ...19921537913
urinary leukocyte esterase test: a screening method for the detection of asymptomatic chlamydial and gonococcal infections in men.the ability of a urinary dipstick leukocyte esterase test (let) to predict culture-verified asymptomatic urethral infections of chlamydia trachomatis and neisseria gonorrhoeae was determined in 1095 men (aged 18-39) who presented to an urban hospital for acute general medical care. prevalence of chlamydial and gonococcal infections were 4% and 2.5% respectively; let sensitivity was 41% and specificity was 91%. in the youngest one-third, the prevalence of infection and let sensitivity and specifi ...19921538163
pathfinder direct fluorescent antigen test for diagnosing maternal chlamydial infections. an evaluation.chlamydia trachomatis is a frequent cause of genital infections in young women. chlamydial infections may adversely affect perinatal outcome. we compared the pathfinder direct fluorescent antigen (dfa) test for chlamydial infections to tissue culture isolation in an obstetric population. among 984 samples, 152 (15.4%) were inadequate for evaluation using the pathfinder dfa test. among 783 evaluable specimen pairs, the sensitivity, specificity, and positive and negative predictive values of the p ...19921538363
[homeopathy and its relation to the unconscious--of the physician]. 20061539121
transjugular intrahepatic portacaval stent shunt as a rescue treatment for life-threatening variceal bleeding in a cirrhotic patient with severe liver failure.variceal bleeding in cirrhotic patients with severe liver failure that is not controllable by endoscopic sclerotherapy is a life-threatening situation. we report the case of a patient with decompensated cirrhosis (pugh class c) who bled repeatedly from gastric varices despite multiple sessions of sclerotherapy. the portal vein was catheterized by a transjugular approach. a tract between a hepatic vein and the portal vein was created after balloon dilatation, and this opening was stented with an ...20061539575
detection of the tight junction-associated protein zo-1 in astrocytes and other nonepithelial cell types.zo-1 is a high molecular mass phosphoprotein peripherally associated with the cytoplasmic surface of tight junctions in epithelial and endothelial cells. we report here that zo-1 is also present in several nonepithelial cell types in vitro that are not believed to form tight junctions, including primary cultures of astrocytes, schwann cells, and dermal fibroblasts and the c6 glioma, s-180 (sarcoma), and p3 myeloma cell lines. immunoblots of cell extracts probed with a zo-1-specific monoclonal an ...19921539634
models for recall and recognition. 20061539943
muscle strength, endurance, and aerobic capacity in rheumatoid arthritis: a comparative study with healthy subjects.isometric/isokinetic muscle strength and isokinetic endurance of the lower extremities as well as aerobic capacity were evaluated in 67 patients (43 female, 24 male; mean age 53 years, range 23-65) with classical/definite rheumatoid arthritis (ra) of functional class ii. results obtained were compared with those of a healthy reference group matched for age and sex. disease characteristics of the group with ra were registered and lifestyle characteristics, such as work load, exercise, diet, smoki ...20061540034
chlamydia trachomatis serovar differentiation by direct sequence analysis of the variable segment 4 region of the major outer membrane protein gene.the polymerase chain reaction method was used to amplify dna from the fourth variable segment of the gene encoding the major outer membrane protein of chlamydia trachomatis. direct sequencing of the amplified dna from prototype strains confirmed previously identified nucleotide sequence differences that were specific for each serovar. this analysis revealed differences in the dna sequences of prototype strains c/uw-1 and g/iol-238 from those of prototype strains c/tw-3 and g/uw-57, sequenced pre ...19921541524
chlamydia trachomatis pneumonia induces in vivo production of interleukin-1 and -6.cytokine induction during chlamydia trachomatis pneumonia may alter the pathogenesis or course of disease. we examined interleukin-1 (il-1) and il-6 production by measuring mrna and bioactivity in murine lungs. mrna and bioactivity for il-1 alpha, il-1 beta, and il-6 increased after chlamydia infection. these cytokines may be important in regulating host defenses against c. trachomatis.19921541536
antigenic analysis of the chlamydial 75-kilodalton protein.both b- and t-cell immunogenicity of a chlamydial 75-kda protein was analyzed by using 131 partially overlapped decapeptide homologs of the 75-kda protein from chlamydia trachomatis serovar l2. six rabbit antiserum specimens raised with serovars b, c, and l2 were used to assay the antibody reactivities of the decapeptides. seventy-five of the 131 decapeptides were recognized by at least one antiserum specimen, and two peptides were found to be immunodominant and surface accessible on native orga ...19921541537
[a case report of coronary arteriovenous fistula between the left coronary artery and superior vena cava].a 52-year-old man with coronary arteriovenous fistula between left coronary artery and superior vena cava was reported. he was admitted to our hospital for chest pain, but had no murmur. the operation was performed without the use of cardiopulmonary bypass. the fistula was ligated near its inlet to the superior vena cava. postoperative course of the patient was uneventful.19921542202
the synapsin i brain distribution in ischemia.we examined the distribution of synapsin i in the gerbil brain and investigated ischemic damage of presynaptic terminals immunohistochemically by using this protein as a marker protein of synaptic vesicles. the reaction for synapsin i in normal gerbil brain is exclusively localized in the neuropil, and other brain structures such as neuronal soma, dendrites, axon bundles, glia and endothelial cells exhibited little immunoreactivity. in a reproducible gerbil model of unilateral cerebral ischemia, ...19871542407
prevalence of urogenital chlamydia trachomatis infection in el salvador. i. infection during pregnancy and perinatal transmission.one-hundred and twenty-nine pregnant women in labour (age range 15-46 years; median age 23) and 42 infants born to chlamydia-positive mothers (age range 5-15 days; median age 10) were investigated to estimate the prevalence and incidence, respectively, of chlamydia trachomatis infection in san salvador, el salvador. urethral and cervical samples were obtained from all women and conjunctival specimens were taken from both eyes of each child. the chlamydial antigen was detected with the commercial ...19921543765
consider chlamydia. 19921546251
[a clinical study of xanthogranulomatous pyelonephritis (xgp) with special emphasis on the magnetic resonance imaging].xanthogranulomatous pyelonephritis (xgp) is an uncommon form of granulomatous inflammation characterized by destruction and replacement of the renal parenchyma by masses of lipid-laden macrophages. we report the first case of the pyonephrotic type of xgp in which magnetic resonance imaging (mri) was used in japan, and summarize the clinical characteristics of 163 cases with xgp in the japanese literature for age, sex, laboratory data, preoperative diagnosis and operation. a 56-year-old female wa ...19831546568
chlamydiazyme plus blocking assay to detect chlamydia trachomatis in endocervical specimens.three methods to detect chlamydia trachomatis in endocervical swab specimens collected from 502 women with genitourinary or abdominopelvic symptoms were evaluated: (1) a direct immunofluorescence assay, (2) an enzyme-linked immunoabsorbent assay, confirming positive samples with a blocking assay, and (3) conventional tissue cell culture. c. trachomatis was detected by at least one method in 72 specimens, of which 56 (11%) were determined to be true-positive results by repeated testing and by per ...19921546689
goya's living skeleton. 19831547443
intravitreal antibiotic therapy with vancomycin and aminoglycoside: examination of the retinal toxicity of repetitive injections after vitreous and lens surgery.the combination of vancomycin and an aminoglycoside antibiotic is frequently injected into aphakic/vitrectomized eyes for the treatment of endophthalmitis. at 2 weeks after lens and vitreous removal, rabbit eyes received an injection of a combination of 1 mg vancomycin and either 400 micrograms amikacin or 100 micrograms gentamicin. furthermore, the effects of repeating the same combination a second and third time at intervals of 48 h were examined by light and transmission electron microscopy a ...19921547968
chlamydia trachomatis detection. 19921548017
the emergence and recurrence of child firesetting: a one-year prospective study.this study provides 1-year, prospective data regarding the firesetting histories of a sample of 138 children (ages 6-13 years) recruited from patient and non-patient populations. parent and child interviews were conducted at initial assessment and 1-year followup. of 78 children initially classified as nonfiresetters, 14 children (18%) later had set a fire. of 60 children initially identified as firesetters, 21 (35%) had set an additional fire by followup. late starting was associated with limit ...19831548392
malpractice cases in other jurisdictions. 19831549251
a developmentally regulated chlamydial gene with apparent homology to eukaryotic histone h1.we have developed a method for the isolation of genes whose expression is developmentally regulated from the murine strain of chlamydia trachomatis. here we describe the identification of two developmental stage-specific genes, one of which is predicted to encode a 26-kda lysine- and alanine-rich protein that appears to be homologous to several eukaryotic histone h1 proteins. a substantial proportion of this homology relates to its distinctive amino acid composition. no sequence homology was obs ...19921549572
peroxidative damage to cardiac mitochondria: identification and purification of modified adenine nucleotide translocase.rat myocardial membranes exposed to free radical-generating systems exhibit both lipid peroxidation and protein alterations. the most sensitive protein, a 28-kda polypeptide, was previously shown to increase slightly in apparent molecular weight before disappearing completely from the protein profile [n. l. parinandi, c. w. zwizinski, and h. h. o. schmid (1991) arch. biochem. biophys. 289, 118-123]. we now report that isolated cardiac mitochondria contain a 28-kda protein which responds in the s ...19921550344
survey of satisfaction with care in a rheumatology outpatient clinic.consumer satisfaction is increasingly recognised by hospital administrators and health care providers as an important aspect of health care. a study was undertaken to investigate the satisfaction with care among patients with rheumatoid arthritis (ra) attending a rheumatology outpatient clinic at leeds general infirmary. the leeds satisfaction questionnaire was developed and rigorously tested for reliability (cronbachs alpha) and stability (test/retest). the leeds satisfaction questionnaire was ...20061550403
interspecies structural diversity among chlamydial genes encoding histone h1.recently, a eukaryotic histone h1-like protein has been detected in chlamydia trachomatis serovar l2 [hackstadt et al., proc. natl. acad. sci. usa 88 (1991) 3937-3941; tao et al., j. bacteriol. 173 (1991) 2818-2822]. we have cloned the corresponding gene from c. trachomatis serovar j and the chlamydia psittaci strain mn. sequencing demonstrated absolute gene identity between the two c. trachomatis serovars l2 and j, but divergence in the c. psittaci strain mn. these differences resulted in alter ...19921551591
the gene for the s7 ribosomal protein of chlamydia trachomatis: characterization within the chlamydial str operon.the prokaryotic ribosomal operon, str, contains open reading frames for the two elongation factors, elongation factor g (ef-g) and elongation factor tu (ef-tu), and ribosomal proteins s7 and s12. the dna sequence and predicted amino acid sequence for s7 from chlamydia trachomatis are presented and compared with homologues from other prokaryotes. also, the relationship of the s7 gene to the open reading frames for ribosomal protein s12 and ef-g is described. significant amino acid homology is als ...19921552847
the natural history of leber's congenital amaurosis. age-related findings in 35 patients.the authors studied 35 patients with leber's congenital amaurosis and assessed visual acuity, fundus appearance, and systemic findings. the patients were arbitrarily divided into five age groups. visual acuities were comparable at all ages. of 22 patients seen for follow-up examinations (mean length of follow-up, 5 years), vision worsened slightly in only 4 patients (3 with macular coloboma-like lesions and 1 with keratoconus). fifty percent of retinal examinations in patients younger than 1 yea ...20031553215
[patent ductus arteriosus in a dog: modified method of double ligation].patent ductus arteriosus, a common congenital cardio-vascular disease, needs surgery for correction. three methods are described: 1. transsection and suturing of the ductus ends; 2. dissection of the ductus itself and occlusion by double ligation (classical method), 3. modified double ligation described by jackson and henderson. a modification of the technique is described, based on the previous methods of double ligation.19921553518
computerized identification of patients at high risk for hospital-acquired infection.surveillance for hospital-acquired infections is required in u.s. hospitals, and statistical methods have been used to predict the risk of infection. we used the help (health evaluation through logical processing) hospital information system at lds hospital to develop computerized methods to identify and verify hospital-acquired infections. the criteria for hospital-acquired infection are standardized and based on the guidelines of the study of the efficacy of nosocomial infection control and th ...19911554148
frequency of ocular chlamydial infection among young adults.young adults are at particular risk for chlamydia trachomatis infection. to determine whether there is a high rate of asymptomatic ocular chlamydial infection in this population, 131 eyes from 72 patients aged 18 to 30 years with no symptoms of conjunctivitis were tested for c. trachomatis by means of mccoy cell culture and a direct enzyme immunoassay. in addition, 51 of the patients underwent serologic testing to detect systemic chlamydial disease. ocular chlamydial infection was not found in a ...19921555129
transforming growth factor-beta induces selective increase of proteoglycan production and changes in the copolymeric structure of dermatan sulphate in human skin fibroblasts.human embryonic skin fibroblasts were pretreated with transforming growth factor-beta (tgf-beta) for 6 h and then labeled with [35s]sulphate and [3h]leucine for 24 h. radiolabeled proteoglycans from the culture medium and the cell layer were isolated and separated by isopycnic density-gradient centrifugation, followed by gel, ion-exchange and hydrophobic-interaction chromatography. the major proteoglycan species were examined by polyacrylamide gel electrophoresis in sodium dodecyl sulphate befor ...19921555588
diagnostic value of some matrix components.although most diagnostic markers lie within the cell, a few are found in the matrix. well-known examples of these are amyloid filaments and fibrous long-spacing collagen (luse body). perhaps not so well-known are the diagnostic values of anchoring fibrils and microvillus-matrix associations. the latter two form the subject of this paper.20061557821
ophthalmia neonatorum in northern norway. i: epidemiology and risk factors.during one year, 1928 of all 1958 neonates born in the county of troms in northern norway were followed until 6 weeks of age, regarding the development of ophthalmia neonatorum. conjunctivitis was found in 364 cases (189 per 1000 neonates), including mild and self-limiting cases. in 14.5 cases per 1000 neonates the ophthalmia neonatorum was recorded as severe. boys were more often affected than girls (p = 0.001). the age of the mother and obstetric events did not significantly influence the occu ...19921557969
ophthalmia neonatorum in northern norway. ii. microbiology with emphasis on chlamydia trachomatis.in a population of 1928 neonates in northern norway, ophthalmia neonatorum was diagnosed in 18.9%, including mild and self-limiting cases. sixteen out of 269 (6.0%) cultured cases were positive for chlamydia trachomatis. no gonococcal ophthalmia was seen. in neonates whose symptoms began in the maternity wards, the distribution of the isolated microorganisms (mainly staphylococcus aureus) was different from those in whom symptoms began after discharge (mainly s. aureus, staphylococcus species (c ...19921557970
ureaplasma urealyticum and large colony mycoplasma colonization in female children and its relationship to sexual contact, age, and race.in order to determine if ureaplasma urealyticum (uu) or large colony mycoplasma (lcm) colonization was related to a history of sexual abuse, the type of sexual contact, an enlarged vaginal introitus transverse diameter (greater than 4 mm), age or race, 452 female children, ages 1-12 years, were evaluated by the child sexual abuse team at wake medical center in raleigh, nc. thirty-six girls were deleted because of inadequate cultures. when controlled for race and age, uu throat (t), vaginal (v), ...19921559174
ofloxacin clinical pharmacokinetics.ofloxacin is a new fluoroquinolone with a spectrum of activity similar to other fluoroquinolones with activity which includes chlamydia trachomatis, mycobacterium spp., mycoplasma spp. and legionella pneumophila. through its additional mechanisms of action, ofloxacin may be less susceptible to the development of resistance from staphylococcus aureus commonly seen with currently available fluoroquinolones. the impact of these findings cannot be evaluated without further clinical experience. the p ...19921559306
molecular cloning and nucleotide sequence analysis of the gene encoding the immunoreactive brucella abortus hsp60 protein, ba60k.a recombinant 60 kda brucella abortus protein expressed in escherichia coli was recognized in immunoblots by sera from mice experimentally infected with b. abortus and a dog experimentally infected with b. canis. sera from humans and dogs with naturally acquired brucellosis also recognized this protein, which was designated ba60k. the gene encoding ba60k was localized within an 18 kb b. abortus genomic fragment and its direction of transcription determined by subcloning and maxicell analysis of ...19921560753
[complication of chlamydia trachomatis infection]. 19921560977
risk factors for tubal infertility. influence of history of prior pelvic inflammatory disease.in order to explore possible etiologic differences between tubal infertility in women who had been physician-diagnosed as having pelvic inflammatory disease ("overt" pid) and in women who had not ("silent" pelvic inflammatory disease), we made use of self-reported data from a large, population-based, case-control study of infertility in king county, washington. responses from 33 infertile women with no history of physician-reported pid and 129 infertile women with such a history were compared to ...19921561585
cost-benefit analysis of selective screening criteria for chlamydia trachomatis infection in women attending colorado family planning clinics.women attending family planning clinics in colorado during 1988 were screened for chlamydia trachomatis infection by enzyme immunoassay (eia, chlamydiazyme, abbott laboratories; abbott park, il). cervical specimens from 11,793 women attending 22 family planning clinics were analyzed. patient history and physical exams were used to assess risk factors for infection. a total of 913 individuals (7.7%) had positive culture results for c. trachomatis. multivariate analysis showed that infection was s ...19921561588
[contraception, abortion and sexually transmitted diseases].contraceptives that protect against pregnancy tend to offer the least protection against sexually transmitted diseases (stds), particularly needed by young people who change partners frequently. oral contraceptive (ocs) protect best against pregnancy and salpingitis, but they do not protect against infections of the cervix; thus, there is a higher incidence of chlamydia trachomatis and neisseria gonorrhea among oc users. the iud is also very effective, but there is an elevated risk of infectio ...19921561591
pathogenesis of endometritis and salpingitis in a guinea pig model of chlamydial genital infection.the development of tubal obstruction and subsequent infertility is a major sequelum of upper genital tract infection with chlamydia trachomatis; however, little is known about the pathogenesis of the infection. in this investigation, the authors present a detailed study of the progression of ascending chlamydial infection in female guinea pigs resulting from intravaginal inoculation of the chlamydia psittaci agent of guinea pig inclusion conjunctivitis (gpic). isolation of chlamydiae from differ ...19921562052
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