Publications

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erythema nodosum associated with shigella colitis. 19853888122
[determination of the adenine-specific methylase recognition site of shigella sonnei 47, using hydrazinolysis with subsequent separation of purine oligonucleotides by thin layer chromatography on deae- cellulose].a procedure for separation of oligopurine blocks of different length and composition by two-dimensional thin layer chromatography on deae-cellulose plates has been developed. this method allows a comparative analysis of the purine isostich content in the dnas of various origin. in case of methylated dna, the method permits to compare the substrate specificity of different enzymes responsible for the adenine residue methylation in the dna. in combination with enzymatic treatment of labeled methyl ...19853888295
[comparative pharmacological analysis of the mechanisms of thrombocyte aggregation induced by bacterial toxins and adp].it has been demonstrated in experiments in vitro that exotoxin and endotoxin of shigella sonnei produce the concentration-dependent rabbit platelet aggregation. acetylsalicylic acid and theophylline substantially inhibit the adp-induced aggregation and suppress to a less degree the effects of bacterial toxins. monoiodine acetate inhibits aggregation induced by adp and potentiates the aggregating action of toxins.19853888666
survival of bacterial enteropathogens in the ice of popular drinks.we examined the survival of four bacterial enteropathogens frozen in ice and subsequently allowed to melt in various popular drinks. the counts of all the organisms were markedly lowered by freezing alone, and the numbers were further decreased by exposure to some of the drinks. nevertheless, none of the organisms were completely eliminated as a result of freezing for 24 hours followed by melting in any of the test drinks, even when the drink was 86-proof tequila.19853889393
treatment of severe bacillary dysentery with trimethoprim alone.trimethoprim (300 mg twice daily for five days) and co-trimoxazole (two tablets twice daily for five days) were compared as treatment for adult patients with severe shigellosis in rwanda. excellent bacteriological and clinical results were obtained with both regimens, with the exception of patients infected with a trimethoprim-resistant strain of shigella dysenteriae type 1. since only 20 patients were investigated, the conclusions of our study do not reach statistical significance. before recom ...19853890282
[epidemiology and clinical aspects of campylobacter enteritis in childhood].stool specimens of 478 children with enteritic symptoms were screened for enteropathogenic bacteria over a 5-month period. 28 cases of infection due to campylobacter jejuni were found. the incidence of campylobacter enteritis exceeded that of salmonella and shigella infections recorded over the same period (17 and 11 cases, respectively). infected children-aged between 2 months and 15 1/2 years-presented with symptoms of mild gastroenteritis. 18 patients had bloody diarrhoea, whilst 4 children a ...19853890373
a comparative study of enterotoxigenic escherichia coli, shigella, aeromonas, and vibrio as etiologies of diarrhea in northeastern thailand.the incidence of enterotoxigenic escherichia coli (etec), shigella, aeromonas, and vibrio was determined in patients with diarrhea seen at a hospital in northeastern thailand, and compared with the incidence of these bacteria in household contacts and their neighbors. etec was identified in 17%, shigella in 9%, aeromonas in 9%, v. parahaemolyticus in 5%, and non-01 v. cholerae in 2% of 299 patients with diarrhea. these five species of bacteria were isolated more often from patients with diarrhea ...19853890578
an epidemic of bacillary dysentery at raipur. 19853891837
[shigella serotypes isolated july-october 1983 and their antibiotic sensitivity].twenty four strains of shigella were isolated from the stool of patients who had been admitted to the hospital with the complaint of diarrhea. of these, 17 were found to be sh. flexneri, and 7 sh. boydii. antibiotic susceptibility tests indicated that all of the 24 isolates probably had multiple resistance factors. twenty three of 24 strains were found to be resistant to ampicillin. this may be important from a therapeutic point of view.19853892249
[the shigella flexneri epidemic of 1983/84: the lesson to be learned from this]. 19853892308
[antibiotic resistance in the isolates of shigella flexneri type 2 associated with the "shrimp epidemic"]. 19853892309
[5 cases of reactive arthritis following a shigella flexneri epidemic in the netherlands]. 19853892310
[bacillary dysentery in utrecht: 2 epidemics]. 19853892311
[clinical observations during an epidemic caused by shigella flexneri]. 19853892312
[detection of shigella antigens in the feces of patients with acute intestinal diseases using a coagglutination reaction].a total of 113 patients with acute intestinal diseases have been examined with the use of the coagglutination test. 84.95% of the patients showed the presence of different shigella antigens. in patients with bacteriologically confirmed dysentery the corresponding shigella antigens were detected in 96.97% of cases in s. sonnei dysentery, in 90% of cases in s. flexneri dysentery, in 75% of cases in s. newcastle dysentery and in 100% of bases in s. boydii dysentery. in 81.6% of patients with acute ...19853892986
etiology and epidemiology of diarrheal diseases in the united states.accurate data on the frequency of acute diarrheal illness and the distribution of pathogens are not available for several reasons, including the facts that only a small fraction of cases come to the attention of physicians and that available diagnostic tests establish an etiology in only about half of these. in a survey of three groups of patients in a community (upper-middle class and lower class outpatients and hospitalized infants), a possible cause was found in fewer than 20 percent of outpa ...19853893118
nonfluid therapy and selected chemoprophylaxis of acute diarrhea.various available forms of therapy can decrease morbidity and mortality associated with acute diarrhea. oral fluids represent the cornerstone of therapy of all cases. a variety of agents acting nonspecifically can decrease diarrhea and improve other worrisome symptoms associated with enteric infection. kaopectate makes the stool more formed but has little additional effects. bismuth subsalicylate, an antisecretory agent, reduces the number of stools passed by about 50 percent and improves other ...19853893119
[chloramphenicol residues and their toxicity]. 19853893302
sequence specificity of isolated dna-adenine methylases from mycobacterium smegmatis (butyricum) and shigella sonnei 47 cells.a set of four individual dna-adenine methylases differing in pi (isoelectric point) values (mmbu4.2, mmbu6.4, mmbu7.3, and mmbu8.7), and a sole methylating enzyme with the same base specificity (msso9.5) are present in m. smegmatis (butyricum) and sh. sonnei 47 cells, respectively. the sequence specificity of each of those was studied 'in vitro' by a combined approach that comprised isostich (purine tract) analysis and identification of the immediate neighbourhood of the methylated base within t ...19853893435
vaccines for bacterial diarrhoeas. 19853893456
improved enrichment for recovery of shigella sonnei from foods.shigella species were recovered from foods by the procedure described in the bacteriological analytical manual, 5th ed. the method is effective if shigella species are present at about 10(6) cells/g. a 25 g food portion was incubated in gram-negative (gn) and selenite cystine broths for 16 h at 35 degrees c and streaked onto macconkey, levine's eosin methylene blue, desoxycholate citrate, and xylose lysine desoxycholate agars. s. sonnei cells were recovered quantitatively at 44.5 degrees c, and ...19853894320
gastroenteritis caused by escherichia coli and shigella retards the growth of children. 19853895056
neonatal shigellosis with bowel perforation. 19853895181
double immunofluorescence microscopic technique for accurate differentiation of extracellularly and intracellularly located bacteria in cell culture.a double immunofluorescence staining technique is described for differentiation between cell-attached (extracellular) and ingested (intracellular) bacteria by hep-2 cells in cell culture monolayers. this method is based upon the observation that membranes of viable mammalian cells are impermeable for antibodies but are rendered permeable by treatment with fixatives. consequently, extracellular bacteria can be stained by specific rhodamine-labeled antibodies before fixation, and intracellular bac ...19853897263
[acute alithiasic cholecystitis caused by infectious gastroenteritis]. 19853897740
[possibilities of enhancing the sensitivity of the coagglutination reaction].the influence of heating shigella suspension at 60 degrees c (for 3 minutes) and 100 degrees c (for 30 minutes), as well as adding extraneous microorganisms (escherichia coli, proteus) and homologous antibodies to these suspensions, on the sensitivity of the coagglutination test has been studied. the possibility of enhancing the sensitivity of this test 10 to 100 times by heating shigella suspensions at 100 degrees c for 30 minutes has been shown.19853898662
[determination of the structural integrity of dna from pathogenic enterobacteria].among the enterobacterial strains under study, more organisms in the stationary phase of growth have been found to have nicks in their dna than those in the exponential phase. bacteria less sensitive to ultraviolet irradiation have the least number of nicks in each phase of growth. the number of nicks in different strains belonging to the serovar is sufficiently stable. virulent and avirulent forms show no difference in this characteristic.19853898668
[structural characteristics of the remote interaction of shigella and salmonella with enterocytes of experimental animals].the electron-microscopic analysis of the adhesion of shigella and salmonella nonfimbriated strains to the surface of enterocytes of laboratory animals in vivo and in vitro has revealed the presence of structural links, whose formation occurs with the participation of the glycocalix of the interacting cells and the extracellular gel.19853898674
plasmid-mediated drug resistance of shigellae in kuwait.of 153 clinical isolates of shigellae examined, 64.7% belonged to shigella flexneri, 18.9% to sh. sonnei, 11.8% to sh. boydii and 4.6% to sh. dysenteriae. part of these isolates were resistant to sulfamethoxazole and streptomycin (88.2% each), ampicillin (66.7%), tetracycline (63.4%) and co-trimoxazole (43.1%), with levels of resistance (mic50 and mic90) being invariably high. resistance to three or more drugs (multidrug resistance) was seen in 77.8% of the isolates. all the 25 strains examined ...19853899007
dna hybridization in the diagnosis of bacterial diarrhea.dna hybridization with either cloned genes for enteropathogenic determinants or dna segments that are species-specific is a valuable tool to identify certain bacterial enteric pathogens. thus far, only e. coli and v. cholerae enterotoxin gene probes have been used to identify etec and v. cholerae in clinical specimens. dna probes developed for salmonella, shigella, campylobacter, and enteroinvasive and enteropathogenic e. coli need to be evaluated with clinical specimens. the major contribution ...19853899478
[interrelation of erythropoiesis and lymphopoiesis in experimental infectious processes and in viral leukemogenesis].interrelation of erythropoiesis and immune response was studied in mice of various lines infected with mycoplasma species, shigella flexneri 2a and rauscher leukemia virus (r-mlv). intensity of erythropoiesis was estimated by the endocolonization data of sublethally irradiated mice and 59fe incorporation, while immune reactivity--by generation of splenic antibody-forming cells in response to immunization with sheep erythrocytes. the inverse correlation between the ability of infectious agents to ...19853899611
four cases of shigella septicemia in israel.four cases of shigella septicemia are presented. in two children infection was caused by shigella flexneri and in two adults, who had underlying diseases which caused immunosuppression, by shigella sonnei and shigella schmitzi. all four patients responded to intravenous antibiotic therapy. in one patient the organism persisted in the stool, but was eliminated after oral administration of chloramphenicol. shigella septicemia is not uncommon and blood cultures should be obtained in suspected cases ...19853899637
epidemiology of shigella boydii serotype 2, a strain indigenous to spain. 19853899639
hla-b27 and clinical aspects of ankylosing spondylitis: results of prospective studies. 19853899917
shigella septicemia in elderly patients. 19853899990
trat gene sequences, serum resistance and pathogenicity-related factors in clinical isolates of escherichia coli and other gram-negative bacteria.the r6-5 plasmid-specified outer membrane protein, trat protein, has previously been shown to mediate resistance to bacterial killing by serum. colony hybridization with a 700 bp dna fragment carrying most of the trat gene was used to examine the prevalence of trat in gram-negative bacteria, particularly strains of escherichia coli, isolated from clinical specimens. trat was found in isolates of e. coli, salmonella, shigella and klebsiella, but not in pseudomonas, aeromonas or plesiomonas, nor i ...19853900279
shigellosis (antibiotic resistance and transfer of r-factor). 19853900357
[functional status of monocytes of the peripheral blood in dysentery]. 19853900573
comparative biology of intracellular parasitism. 19853900672
[effectiveness of thymalin and prodigiozan in the complex treatment of patients with dysentery]. 19853901509
[local immunity in the parenteral immunization of guinea pigs with a ribosomal dysentery vaccine].after immunization of guinea pigs with shigella sonnei ribosomal vaccine o-antibodies appeared not only in the blood serum of the animals, but also in their lacrimal fluid. since no correlation between the levels of serum and secretory antibodies was detected and since the time course of changes in these antibody levels was quite different (serum antibodies reached their peak on day 7 while secretory antibodies, on day 14 after vaccination), antibodies in lacrimal fluid were supposed to reflect ...19853901625
epidemiological markers of shigella sonnei infections: r-plasmid fingerprinting, phage-typing and biotyping.in 1980, the number of shigella sonnei strains isolated in sicily increased markedly. approximately 80% of the isolates belonged to phage-type 3 and showed the same antibiotic resistance pattern, suggesting that an epidemic had been going on for several months. plasmid analysis of strains isolated at various times in different places supported this view. agarose gel electrophoresis of plasmid preparations from seven selected phage-type 3 isolates showed the presence of two plasmids, of 80 megada ...19853901879
sensitivity to bile salts of shigella flexneri sublethally heat stressed in buffer or broth.batch cultures of shigella flexneri m4243 were grown at 37 degrees c in broth to early stationary phase, washed, and heated at 50 degrees c in 0.1 m phosphate buffer (ph 7.0). cells were surface plated on a tryptic phytone glucose agar (tpga), tpga with 0.15 or 0.85% bile salts no. 3 (tpga-bs 0.15 or tpga-bs 0.85), or tpga with 0.25 or 0.50% sodium deoxycholate (tpga-dc 0.25 or tpga-dc 0.50). cells sampled after no heating produced colony counts on tpga-bs 0.85 or on tpga-dc 0.50 that were no mo ...19853901919
changes in virulence of waterborne enteropathogens with chlorine injury.we designed experiments to assess the effect of chlorine injury on the virulence of waterborne enteropathogens. higher chlorine doses (0.9 to 1.5 mg/liter) were necessary to produce injured yersinia enterocolitica, salmonella typhimurium, and shigella spp. than to produce injured enterotoxigenic escherichia coli or coliform bacteria (0.25 to 0.5 mg/liter) in the test system used; 50% lethal dose experiments in which mice were used showed that injured y. enterocolitica cells were 20 times less vi ...19853901920
susceptibility and hla-b27 in post-dysenteric arthropathies.a recent outbreak of bacillary dysentery in the netherlands revealed that, despite the close association of hla-b27 with post-dysenteric or reactive arthritis (rea), not even in one family did all hla-b27 positive patients infected by an arthritogenic bacterium, develop rea. this dissociation shows that additional factors beside b27 may determine susceptibility to rea.19853902627
oral vaccination against enteric bacterial infections: an overview.the present situation and the future prospects for the use of oral vaccines against the major enteric diseases typhoid fever, shigellosis and cholera are discussed in this paper. no significant protection could be demonstrated for oral inactivated whole-cell vaccines. in contrast, an oral live vaccine based on the attenuated salmonella typhi strain ty 21a was highly efficacious in volunteer challenge studies and in a controlled field trial. two attenuated strains are presently being tested in vo ...19853902655
[functional status of the small intestine in dysentery sonnei and flexneri]. 19853904028
[fractionation and purification of dna-methylases from shigella sonnei 47].possible applications of various column chromatography techniques and isoelectrofocusing for the study of dna-methylases of shigella sonnei 47 cells were analyzed. a simple, rapid and convenient procedure based on the use of cation-exchange chromatography was developed for obtaining a highly active total preparation of methylases. affinity chromatography on heparin-sepharose was shown to be a promising approach for separating methylases according to their specificity towards nitrous bases. isoel ...19853904838
decreased chemiluminescent associated phagocytic response of peripheral blood mononuclear cells to chlamydia trachomatis in patients with hla-b27+ anterior uveitis.the chemiluminescent (cl) associated phagocytic response of peripheral blood monocytes to two serovars of chlamydia trachomatis, shigella flexneri and zymosan was assessed in a group of 26 patients with anterior uveitis (au). hla-b27+ patients with au, when compared to hla-b27- patients with au and appropriate controls, had a significantly decreased cl response to c. trachomatis but no difference between groups in the response to s. flexneri and zymosan. the decreased chemiluminescent (phagocyti ...19853904888
identification and antigenic characterization of virulence-associated, plasmid-coded proteins of shigella spp. and enteroinvasive escherichia coli.seven plasmid-coded polypeptides, designated a through g, were identified by two-dimensional nonequilibrium ph gradient electrophoresis of radiolabeled extracts from minicells of virulent shigella flexneri serotypes 2a and 5 and enteroinvasive escherichia coli o143. these polypeptides were deemed to be products of 140-megadalton (mda) virulence-associated plasmids because they were not synthesized in minicells which were not harboring a 140-mda plasmid or in minicells which were carrying an f la ...19853905608
characterization of monoclonal antibodies against shiga-like toxin from escherichia coli.three monoclonal antibodies, designated mab 16e6, mab 13c4, and mab 19g8, were produced which recognize shiga-like toxin (slt) from escherichia coli. all three monoclonal antibodies neutralized the cytotoxicity of e. coli slt and were able to immunoprecipitate intact labeled toxin with staphylococcus aureus protein a. the three antibodies were of the g1 heavy and kappa light chain classes. mab 16e6 bound to the b subunit of slt in western blots and also neutralized the lethality of the toxin for ...19853905611
interspecific reconstitution of maltose transport and chemotaxis in escherichia coli with maltose-binding protein from various enteric bacteria.in escherichia coli, the periplasmic maltose-binding protein (mbp), the product of the male gene, is the primary recognition component of the transport system for maltose and maltodextrins. it is also the maltose chemoreceptor, in which capacity it interacts with the signal transducer tar (taxis to aspartate and some repellents). in studies of the maltose system in other members of the family enterobacteriaceae, we found that mbp is produced by salmonella typhimurium, klebsiella pneumoniae, ente ...19853905762
transferable resistance to trimethoprim in enteric pathogens isolated in kuwait.trimethoprim resistance was seen in 14.8% of 500 strains of salmonella, 43.1% of 153 strains of shigella and 59% of 27 strains of escherichia coli isolated from stools of patients with diarrhoea. strains with a high level of trimethoprim resistance (mic of much greater than 512 micrograms) were subjected to conjugal transfer. trimethoprim resistance was plasmid-mediated in all of 42 strains of shigella and 12 strains of e. coli examined. however, 2 of the 47 strains of salmonella could not trans ...19853905958
changing pattern of resistant shiga bacillus (shigella dysenteriae type 1) and shigella flexneri in bangladesh.shigella dysenteriae type 1 (shiga bacillus) has made a dramatic comeback in bangladesh after 10 years when shigella flexneri was the dominant serogroup. whereas s. flexneri showed little increase in resistance to the commonly used antimicrobial agents--namely, trimethoprim-sulfamethoxazole (tmp-smx) and ampicillin-over the years, s. dysenteriae type 1 underwent rapid changes with regard to sensitivity pattern during the last two years. the first tmp-smx-resistant strain of s. dysenteriae type 1 ...19853905980
the changing pattern of resistance to ampicillin and co-trimoxazole in shigella serotypes in bangalore, southern india. 19853905991
[3-year study of shigellosis epidemic in rwanda, central africa. problems of public health and bacteriological aspects].since 1979, the lakes region (kivu in zaïre, western uganda, rwanda, burundi) has been stricken by a bacillary dysentery epidemic at s. dysenteriae type 1. supposed to be extinct since the 1950s., this bacillus has revealed a very noticeable virulence as well by the number of registered cases (524/100.000 in 1983 at ruhengeri-rwanda) as its fast break through. demographic density (from 400 to 600/km2) has played an important role and has facilitated the transition toward epidemicity. systematic ...19853906345
acute childhood diarrhoea in naples: an aetiologic study.a potential aetiologic agent was detected in the stools of 56% of 118 children hospitalized in naples for acute diarrhoea. rotavirus and salmonella were the agents most commonly associated with disease, accounting for 23 and 17 percent of cases, respectively. campylobacter jejuni, enterotoxigenic escherichia coli, yersinia and shigella were less frequently isolated (total, 11% of cases). these findings fit well with the epidemiological picture described for other developed countries, except for ...19853906366
[ribosomal dysentery vaccine. study of response and antigenic activity in healthy volunteers].in earlier studies shigella sonnei ribosomal vaccine was shown to be highly protective for guinea pigs and monkeys. the object of the present study, carried out in 20 healthy volunteers, was the safety and the antigenic activity of this vaccine. the subcutaneous injection of the ribosomal vaccine in doses of 100 micrograms and 200 micrograms produced no febrile reactions nor biochemical and histological changes. the minimal local reaction was observed after injection into the subscapular region: ...19853907215
[the nature of the cyclic course of the epidemiological process in dysentery]. 19853907219
shigella and salmonella species from kigali (rwanda) (1976-1982). 19853907525
[site-specificity of dna methylases from shigella sonnei 47 cells].a complex approach involving isoplith analysis, enzymatic treatment of methylated isopliths and a computer analysis of experimental data has been used for determining site specificity of six methylases from shigella sonnei 47 cells termed according to their specificity for a nitrous base and pi as mc4.2, mc5.3, mc6.2, mc7.4, mc8.4 and ma9.5. it has been found that the recognition site of ma9.5 is a palyndrome six-member structure of the 5'...gaattc...3' type and that this enzyme is an isometimer ...19853907723
outbreak of shigella dysenteriae type-1 dysentery in himachal pradesh--aetiologic investigation in distt: shimla. 19853908554
[significance of isolating shigella under epidemiological circumstances]. 19853911318
failure to detect antihuman-immunoglobulinallotype-active antibodies in diagnostic sera directed against some enterobacteria.diagnostic sera to determine antigenic properties of bacteria were tested to clarify the question whether these sera also contain antibodies being active against human immunoglobulin allotypes. sera directed against various strains of escherichia coli, salmonella, and shigella were found to be negative for anti-ig-allotype activity.19853911645
[effect of r plasmids on the growth characteristics of shigella sonnei and escherichia coli].revealing of growth characteristics in plasmid and plasmid-free strains was studied with the use of two different hosts: e. coli 15-3 and sh. sonnei 11-941 containing conjugative r plasmids differing in the set of the resistance markers. it was shown that the r plasmids had no noticeable effect on the period of the lag phase and the time of the microbial cell generation. it was also shown that the number of the viable cells in separate cultures of the plasmid-free strain of sh. sonnei 11-941 and ...19853911872
[preventing bacillary dysentery with magnetized drinking water]. 19853912058
[epidemiologic survey of an outbreak of food-borne bacillary dysentery]. 19853912060
plasmid characterization of shigella spp. isolated from children with shigellosis and asymptomatic excretors.strains of shigella dysenteriae type 1 and sh. flexneri isolated during 1981-82 from children with shigellosis and also from asymptomatic excretors were examined for drug resistance and for the presence of plasmids. shigella strains isolated from dysenteric children showed multiple plasmid bands. thirty-eight percent of the strains transferred the drug resistance factor(s), either partially or fully to a recipient escherichia coli k12 strain. plasmids in the molecular weight range from between 4 ...19853912364
new enteric vaccines: application of new knowledge of receptors and recognition in enteric infections.advances in understanding of the "receptors and recognition" mechanisms of virulence factors of enteric pathogens have been important in the development of enteric vaccines. sophisticated techniques of molecular biology have proved essential to this endeavour. this review summarizes progress in development of vaccines against disease due to vibrio cholerae, escherichia coli, salmonella typhi, shigella and rotavirus enteric infections. all of these vaccines are undergoing, or are about to undergo ...19853913065
shigella flexneri bacteraemia in a patient with acquired immune deficiency syndrome. 19853913261
[vaccines against diarrheal diseases of bacterial origin]. 19853914235
[intestinal cytoprotection]. 19853914439
studies on specificity of protection induced by immunization with outer membrane proteins of shigella.immunization of c3h/hej mice with outer membrane proteins (omp) and with peptidoglycan associated proteins (pgp) isolated from shigella flexneri 3a and from shigella sonnei phase i protected the animals against lethal dose of homologous and heterologous bacteria and against various serotypes of shigella flexneri. neither of the protein preparations protected the animals against challenge with escherichia, klebsiella, citrobacter, salmonella, serratia, proteus and pseudomonas. omp preparations ho ...19853914866
transfer of immunity by means of spleen cells from mice immunized with outer membrane proteins of shigella flexneri.intraperitoneal immunization of mice with a single dose (5 micrograms) of outer membrane proteins (omp) of shigella flexneri was found to evoke in the spleen the appearance of cells by means of which immunity to lethal dose of shigella could be transferred into other mice. active cells capable of transferring immunity appeared in the spleen of the animals as early as on day 3, reached the strongest protective activity on day 4 and disappeared on day 8 after immunization. active cells from animal ...19853914867
[contamination by micro-organisms of health interest in the waters of the imperial canal of aragón]. 19853915153
[epidemiological analysis of the outbreak of bacillary dysentery in xinjiang]. 19853915444
[emergence of resistance to antibiotics in enteropathogens. implications and speculations]. 19853915679
emergence of sulfamethoxazole-trimethoprim resistant shigella flexneri in northeastern brazil.in contrast to prior experience in this setting, three of four shigella flexneri strains recently isolated from patients in northeastern brazil with acute inflammatory diarrhea were found to be resistant to sulfamethoxazole, trimethoprim and the combination in vitro. we performed mating studies to determine if the resistance was transferable, and then isolated and characterized plasmid dna from the resistant shigella isolates, other resistant enterobacteriaceae isolated simultaneously from the s ...19853915680
[shigellemia in a patient with the septic variant of the course of dysentery (clinical case)]. 19853916350
[incidence of enterovirulent escherichia coli in acute diarrheal diseases in preschool children in the area of novi sad]. 19853916706
[effect of sulfonamides on the fatty acid composition of enterobacterial lipids]. 19853917183
effect of human gamma interferon on invasiveness of salmonella typhimurium in hep-2 cell cultures.the effect of human gamma interferon (huifn-gamma) on the invasiveness of salmonella typhimurium, shigella flexneri and yersinia enterocolitica serotype 0:3 in hep-2 cell cultures was examined. the intracellular and extracellular bacteria were identified by a combination of nomarski differential interference contrast microscopy and uv incident light microscopy applied on the same microscope. pretreatment of hep-2 cells with huifn-gamma reduced in a dose dependent manner the number of s. typhimur ...19853921632
properties of human anti-lipopolysaccharide gamma globulin: specificity and protective effects.blood donated to the natal blood transfusion service was screened by an enzyme-linked immunosorbent assay (elisa) for anti-lipopolysaccharide (anti-lps) antibodies. plasma units with high concentrations (greater than 40 micrograms/ml) of anti-lps igg were pooled and fractionated to obtain a gamma globulin (lot lg-1). the binding of lg-1 antibodies to lps prepared from 14 bacterial species and strains was found to be the highest to lps from shigella flexneri, salmonella abortus equi and salmonell ...19853922117
[occurrence of enteritis-causing agents in private households--a pilot study].the increasing occurrence of infectious enteritis caused us to perform a prospective, epidemiological pilotstudy in households, in order to receive informations about the practicability of such a project and the bacteriology of food prepared at home as well. 10 families were asked to gather samples of all foodstuffs consumed during the day in separate containers and to keep them frozen at - 20 degrees c until collected by us once a week. thus we received 4.683 samples within a 6-months-period. i ...19853922161
bactericidal properties of a new water disinfectant.the n-chloramine compound 3-chloro-4,4-dimethyl-2-oxazolidinone (agent i) has been compared with calcium hypochlorite as to its efficacy as a bactericide for the treatment of water. the study included concentration, contact time, ph, temperature, and water quality as controlled variables. the species of bacteria tested were staphylococcus aureus, pseudomonas aeruginosa, and shigella boydii. in general, for highly pure, demand-free water, calcium hypochlorite was the more rapid disinfectant at a ...19853922300
campylobacter jejuni infection in colorado: unexplained excess of cases in males.between january 1, 1981, and december 31, 1982, the colorado department of health received reports of 1,185 culture-confirmed cases of campylobacter jejuni infection. incidence rates were highest among infants less than 1 year old and among persons aged 20-29 years. the distribution of cases by sex showed a predominance among males at all ages except 40-59 years, the most marked predominance occurring in infants under 1 year. the higher rates for males were also significant for all ages combined ...19853923544
infections in british clinical laboratories, 1982-3.during 1982-3, 31 specific and 12 uncharacterized infections were reported from 30 of 240 laboratories, representing 29 223 person-years of experience. thirteen cases of hepatitis included 10 of type b or non-a, non-b hepatitis of probable occupational origin (attributable incidence 34.2 per 100 000 person years) affecting haematology, biochemistry, and postmortem workers. of nine cases of tuberculosis, three were probably acquired in the laboratory (attributable incidence 10.3 per 100 000 perso ...19853926828
evaluation of a new screening system for enteric pathogens.the two-hour rapid sst strip (dms laboratories, inc.) was compared with our standard screening system (triple sugar iron agar, lysine iron agar, and urea) for enteric pathogens. we tested 50 stock cultures of enteric pathogens and 213 stool cultures received in the barnes hospital clinical microbiology laboratory over a two-month period. all enteric pathogens from the stock cultures and clinical specimens were identified correctly with the rapid sst system. more false-positive reactions were obs ...19853928238
in vitro pyocin activity of pseudomonas aeruginosa strains pretreated with antibiotics.fifty-six selected strains of pseudomonas aeruginosa belonging to 8 different pyocin types (h, i, 15, 6, pti-1, pti-2, pti-3, pti-4) were treated with subinhibitory concentrations (mic/2) of either gentamicin or carbenicillin. both treatments induced changes in pyocin patterns for all types but at different levels. the percentage of strains that retained their pyocin pattern were more or less equal in both treatments. in treated and untreated producers, the growth inhibition ability for 5 differ ...19853928466
the in vitro activity and beta-lactamase stability of cefpirome (hr 810), a pyridine cephalosporin agent active against staphylococci, enterobacteriaceae and pseudomonas aeruginosa.the in vitro activity of cefpirome, a new cyclopyridinium cephalosporin, was evaluated against 947 aerobic and anaerobic bacteria. cefpirome inhibited 90% of escherichia coli, klebsiella spp., citrobacter diversus, morganella morganii, proteus vulgaris, proteus mirabilis, aeromonas spp., salmonella spp., shigella spp. and haemophilus and neisseria species at less than or equal to 0.4 mg/l. it had activity comparable to that of cefotaxime, ceftizoxime, ceftazidime, aztreonam, and moxalactam again ...19853928497
[study on an intestinal loop test of virulent shigella bacillus]. 19853932548
identification of 5-acetamido-3,5,7,9-tetradeoxy-7-[(r)-3-hydroxybutyramido]-l- glycero-l-manno-nonulosonic acid as a component of bacterial polysaccharides. 19853933831
colicin e2 production and release by escherichia coli k12 and other enterobacteriaceae.previous work has shown that escherichia coli k12 cole2+ cells undergo a form of partial lysis and exhibit increases in lysophosphatidylethanolamine (lysope) and free fatty acid content due to activation of phospholipase a when induced to produce and release colicin e2. the increase in lysope content was assumed to be essential for efficient colicin release. these same characteristics are also presented by some natural cole2+ isolates, and by other representatives of the enterobacteriaceae after ...19853934329
[enhanced effectiveness of antibiotic therapy with an exogenous lysozyme in dysentery in children].the course of dysentery and the host immune responsiveness were studied in 171 children with acute flexner's and sonne dysentery. 67 children were treated with monomycin in combination with lysozyme and 104 children with monomycin alone. it was shown that the recovery period in children treated with monomycin and lysozyme was shorter as compared to that in children treated with monomycin alone. the immunity characteristics in children treated with monomycin were closer to the physiological value ...19853936408
[virulence of shigella: epithelial cell invasiveness and other dysentery-producing factors]. 19853938489
comparison of loperamide with bismuth subsalicylate for the treatment of acute travelers' diarrhea.loperamide hydrochloride was compared with bismuth subsalicylate for the treatment of acute nondysenteric travelers' diarrhea in 219 students visiting seven countries in latin america. subjects whose condition was not improved with therapy could elect to take trimethoprim-sulfamethoxazole. persons receiving loperamide passed fewer unformed stools when compared with the bismuth subsalicylate group during the first four hours of therapy, from four to 24 hours, and from 24 to 48 hours after therapy ...19863944976
breast feeding as a determinant of severity in shigellosis. evidence for protection throughout the first three years of life in bangladeshi children.little is known about the effect of breast feeding upon the severity of illness due to specific diarrheal pathogens. using a systematically sampled and evaluated population of children aged less than 3 years, who attended a diarrheal disease hospital in bangladesh, the authors performed a case-control study that assessed whether breast feeding reduces the severity of illness in shigellosis. from 540 children presenting with shigellosis between 1980-1982, they created a group of cases (n = 53) wi ...19863953549
risk factors for diarrhoeal deaths: a case-control study at a diarrhoeal disease hospital in bangladesh.the study of diarrhoeal mortality risk has been limited to descriptive epidemiological investigations. this is the first case-control study in this area, and our results show that certain risk factors are predictive of diarrhoeal deaths. we have compared the disease history, associated complications, signs, symptoms and laboratory values of 346 patients who died on the medical wards of dhaka hospital and that for 346 matched controls to identify the risk factors for mortality. patients presentin ...19863957532
ionic, hydrophobic, and hemagglutinating properties of shigella species. 19853965592
modulation of in vitro natural cell-mediated activity against enteropathogenic bacteria by simple sugars.lymphoid cells from mouse peyer's patches and spleens were tested in a 2-h in vitro assay for their natural activity against the enteropathogenic bacteria salmonella typhimurium, salmonella enteritidis, salmonella tel aviv, and shigella sp. x16. the antibacterial activity expressed by normal cells was detected against all the bacterial strains tested with the exception of peyer's patch lymphocytes against s. tel aviv and splenocytes against shigella sp. x16. to determine whether the different ex ...19853967926
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