Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| rt-pcr amplification of rna extracted from formalin-fixed, paraffin-embedded oral cancer sections: analysis of p53 pathway. | we present a new approach towards the detection of the mrnas in formalin-fixed, paraffin-embedded samples using a reverse transcriptase (rt)-polymerase chain reaction (pcr). the total rnas were extracted from 10-micron-thick sections and were reverse-transcribed, then the rt-products were subjected to pcr amplification of gapdh mrna for screening the mrna degradation. next, nested pcr was performed for examining the expression of p53-related genes, p21waf1, mdm2, p33ing1 and p14arf. gapdh mrna e ... | 2003 | 12926130 |
| rapidly enlarging growth of the proximal nail fold. | squamous cell carcinoma (scc) of the nail unit is a rare but often underappreciated neoplasm. treatment can be delayed due to confusion with paronychia and verruca. infection with human papilloma virus (hpv) has been associated with this malignancy. | 2003 | 12930348 |
| solution measurement of dna curvature in papillomavirus e2 binding sites. | 'indirect readout' refers to the proposal that proteins can recognize the intrinsic three-dimensional shape or flexibility of a dna binding sequence apart from direct protein contact with dna base pairs. the differing affinities of human papillomavirus (hpv) e2 proteins for different e2 binding sites have been proposed to reflect indirect readout. dna bending has been observed in x-ray structures of e2 protein-dna complexes. x-ray structures of three different e2 dna binding sites revealed diffe ... | 2003 | 12930964 |
| viruses and the 26s proteasome: hacking into destruction. | the discovery that the human papillomavirus e6 oncoprotein could direct the ubiquitination and degradation of the p53 tumour suppressor at the 26s proteasome was the beginning of a new view on virus-host interactions. a decade later, a plethora of viral proteins have been shown to direct host-cell proteins for proteolytic degradation. these activities are required for various aspects of the virus life-cycle from entry, through replication and enhanced cell survival, to viral release. as with onc ... | 2003 | 12932734 |
| determinants of clearance of human papillomavirus infections in colombian women with normal cytology: a population-based, 5-year follow-up study. | little is known about the factors that influence clearance of human papillomavirus (hpv), the primary cause of cervical carcinoma. a total of 227 women cytologically normal and hpv positive at baseline were identified from a population-based cohort of 1,995 bogota, colombia, women aged 13-85 years followed between 1993 and 2000 (mean follow-up, 5.3 years). hpv dna detection and viral load determination were based on a gp5+/gp6+ polymerase chain reaction enzyme immunoassay. rate ratio estimates f ... | 2003 | 12936904 |
| a p53-dependent, telomere-independent proliferative life span barrier in human astrocytes consistent with the molecular genetics of glioma development. | an in vitro model, based on normal (primary) human astrocytes (nhas), was used to investigate the nature of the selection pressures for events that occur during the progression of astrocyte-derived tumors and, in particular, the potential role of proliferative life span barriers (plbs). as with fibroblasts, nhas senesced with elevated p21(waf1) and senescence-associated beta-galactosidase activities. unlike fibroblasts, replicative senescence (m1) occurred much earlier, after approximately 20 pd ... | 2003 | 12941806 |
| are smoking and chlamydial infection risk factors for cin? different results after adjustment for hpv dna and antibodies. | to identify the risk factors for cervical intraepithelial neoplasia (cin), we reanalysed the data from our previous case-control study by adjusting for human papillomavirus (hpv) antibodies. unlike our previous study based only on hpv dna, smoking and chlamydia trachomatis infection were revealed as significant risk factors for cin after adjustment for hpv antibodies. | 2003 | 12942113 |
| ki-67 immunocytochemistry in liquid based cervical cytology: useful as an adjunctive tool? | to test the ability of ki-67 to detect cytological lesions in a screening setting and its use as a surrogate marker of human papillomavirus (hpv) infection. | 2003 | 12944552 |
| risk of invasive cancer of the cervix in relation to the use of injectable progestogen contraceptives and combined estrogen/progestogen oral contraceptives (south africa). | cervical cancer is caused by specific types of the human papilloma virus (hpv), but not all infected women develop cancer. it has been hypothesized that hormonal contraceptives may potentiate the oncogenicity of hpv infection. | 2003 | 12946044 |
| decreased expression of human papillomavirus e2 protein and transforming growth factor-beta1 in human cervical neoplasia as an early marker in carcinogenesis. | human papillomavirus (hpv) is thought to be one of the possible causative factors in cervical carcinogenesis, and cervical carcinoma cells are refractory to tumor transforming growth factor (tgf)-beta1. the purpose of this study is to investigate the possible cause-effect association between hpv and tgf-beta1 during cervical tumorigenesis. | 2003 | 12949986 |
| evidence for at least three alternative mechanisms targeting the p16ink4a/cyclin d/rb pathway in penile carcinoma, one of which is mediated by high-risk human papillomavirus. | a comprehensive analysis of 53 penile carcinomas was performed to determine which mechanisms might be involved in the disruption of the p16(ink4a)/cyclin d/rb pathway. to that end, human papillomavirus (hpv) presence, p16(ink4a) expression and promoter methylation, and expression of the bmi-1 polycomb gene product were studied. sixteen (30%) of the carcinomas were found to harbour high-risk hpv dna, 15 of which contained hpv 16. hpv 16 e6/e7 oncogene transcripts were detected in 13 (87%) of the ... | 2003 | 12950023 |
| role of hpv dna testing in predicting cervical intraepithelial lesions: comparison of hc hpv and ish hpv. | human papillomavirus (hpv) is widely accepted as the primary agent involved in the development of squamous intraepithelial neoplasia and cervical carcinoma. several commercial tests are available for detecting hpv dna. this study compares the efficacy of inform hpv (in situ hybridization [ish] hpv) and hcii (hc hpv) in predicting cervical lesions. a total of 762 sequential papanicolaou (pap) smears determined by cytologic examination to be either atypical squamous cells of undetermined significa ... | 2003 | 12951683 |
| the clinician's view: role of human papillomavirus testing in the american society for colposcopy and cervical pathology guidelines for the management of abnormal cervical cytology and cervical cancer precursors. | the american society for colposcopy and cervical pathology (asccp) national consensus conference for the management of women with cervical cytological abnormalities and cervical cancer precursors was held on the national institutes of health campus in bethesda, md, september 6-8, 2001. the conference was attended by 121 representatives from 29 national organizations interested in cervical cancer screening issues. for the first time, guidelines for the management of women with abnormal cervical c ... | 2003 | 12952506 |
| association of gp91phox homolog nox1 with anchorage-independent growth and map kinase-activation of transformed human keratinocytes. | among five members of the nadph oxidase (nox) family, nox1 confers mitogenic properties and is implicated to participate in the process of cell transformation. we have established two phenotypes of carcinogenesis model by ethanol treatment of human gingival keratinocytes immortalized with e6/e7 oncogenes of human papillomavirus type16: immortalized (epi) nontransformed cells with epithelium-like morphology and more advanced transformed (fib) cells with spindle fibroblastic-shape morphology. fib ... | 2003 | 12955083 |
| adenocarcinoma of the uterine cervix: the presence of human papillomavirus and the method of detection. | effective screening programs have contributed to a decrease in the incidence of cervical squamous cell carcinomas but have had a limited sensitivity in the detection of adenocarcinoma precursor lesions. the aim of our study was to analyze cervical adenocarcinoma in greater detail: symptoms preceding the detection, the method of detection and the prevalence of human papillomavirus (hpv) with respect to age at diagnosis. | 2003 | 12956848 |
| pyrazoloacridine is active in multidrug-resistant neuroblastoma cell lines with nonfunctional p53. | the purpose of this study was to determine the activity of pyrazoloacridine (pza) in neuroblastomas that have acquired high-level resistance to multiple drugs (not associated with multidrug resistance-associated protein or p-glycoprotein) during therapy, including those with loss of p53 function. | 2003 | 12960142 |
| oridonin induces growth inhibition and apoptosis of a variety of human cancer cells. | pc-spes is an eight herbal mixture that was shown to have activity against prostate cancer. recently, we purified oridonin from rabdosia rubescens, one component of pc-spes, by high performance liquid chromatography (hplc). the ability of oridonin to inhibit the proliferation of cancer cells was examined by mtt assay. oridonin effectively inhibited the proliferation of a wide variety of cancer cells including those from prostate (lncap, du145, pc3), breast (mcf-7, mda-mb231), non-small cell lung ... | 2003 | 12964003 |
| hpv infection and number of lifetime sexual partners are strong predictors for 'natural' regression of cin 2 and 3. | the aim of this paper was to evaluate the factors that predict regression of untreated cin 2 and 3. a total of 93 patients with colposcopic persistent cin 2 and 3 lesions after biopsy were followed for 6 months. human papillomavirus (hpv) types were determined by polymerase chain reaction at enrolment. we analysed the biologic and demographic predictors of natural regression using univariate and multivariate methods. the overall regression rate was 52% (48 out of 93), including 58% (22 out of 38 ... | 2003 | 12966426 |
| mrna splicing regulates human papillomavirus type 11 e1 protein production and dna replication. | the papillomavirus replicative helicase e1 and the origin recognition protein e2 are required for efficient viral dna replication. we fused the green fluorescent protein (gfp) to the human papillomavirus type 11 e1 protein either in a plasmid with the e1 coding region alone (nucleotides [nt] 832 to 2781) (pgfp-11e1) or in a plasmid containing both the e1 and e2 regions (nt 2723 to 3826) and the viral origin of replication (ori) (p11rc). the former supported transient replication of an ori plasmi ... | 2003 | 12970406 |
| direct activation of cyclin-dependent kinase 2 by human papillomavirus e7. | addition of human papillomavirus (hpv) e7 cdk2/cyclin a or cdk2/cyclin e, purified from either insect cells or bacteria, dramatically upregulates histone h1 kinase activity. activation is substrate specific, with a smaller effect noted for retinoblastoma protein (rb). the cdk2 stimulatory activity is equivalent in high-risk (hpv type 16 [hpv16] and hpv31) and low-risk (hpv6b) e7. mutational analyses of hpv16 e7 indicate that the major activity resides in amino acids 9 to 38, spanning cr1 and cr2 ... | 2003 | 12970441 |
| interaction between the hpv e7 oncoprotein and the transcriptional coactivator p300. | infection with high-risk human papillomaviruses (hpv) can lead to the development of cervical cancer. this process depends on the interaction of the virus-encoded oncoproteins, e6 and e7, with a variety of host regulatory proteins. as e7 shares both functional and structural similarities with the adenovirus e1a (ad e1a) protein, we were interested in investigating the possible interactions between e7 and the transcriptional coactivator p300, since it was originally identified as a target of ad e ... | 2003 | 12970734 |
| imiquimod. | the imidazoquinoline, imiquimod, is a low molecular weight, synthetic immune response modifier that is used for the treatment of external genital and perianal warts. it is formulated in a 5% vanishing cream as aldara. this self-applied therapy has shown good efficacy and safety in the treatment of external genital and perianal warts caused by human papillomavirus (hpv) infection. the antiviral mechanism of action of this compound is unlike any other approved antiviral therapy in that it induces ... | 1999 | 12973395 |
| [population-based study of human papillomavirus infection in high-risk area for cervical cancer in shanxi province, china]. | to investigate the prevalence of oncogenic type of human papillomavirus (hpv) infection and identify the high risk population for conducting immuno/chemoprevention of cervical cancer. | 2003 | 12974078 |
| human papillomaviruses in the normal oral cavity of children in japan. | the purpose of this study was to determine the frequency of human papillomavirus (hpv) infections in the normal oral cavity of children in japan. oral squamous cell specimens were collected from 77 children (44 boys and 33 girls), aged 3 and 5 years. extracted dna was evaluated for hpv infections by polymerase chain reaction (pcr) methods, using consensus primers for the l1 region, specific primers, and direct dna sequencing analysis. thirty-seven of 77 specimens (48.1%) were positive for hpv dn ... | 2003 | 13679205 |
| [the effect of human papilloma virus extracts on rabbit endothelial explants]. | 1963 | 14064685 | |
| x chromosome inactivation in cervical cancer patients. | development of cervical carcinomas is strongly associated with presence of human papilloma virus (hpv). recently we found that young patients with breast cancer had a higher frequency of skewed x inactivation in peripheral blood cells, indicating an effect of x-linked genes on breast cancer development. in this study, we investigated the frequency of skewed x-inactivation pattern in blood and tumor biopsies from patients with cervical cancer. no difference in the frequency of skewed x inactivati ... | 2003 | 14499700 |
| [screening and therapy of anal intraepithelial neoplasia (ain) and anal carcinoma in patients with hiv-infection]. | anal intraepithelial neoplasia (ain) is a potential precursor of invasive anal carcinoma. introduction of highly active antiretroviral therapy (haart) in the treatment of hiv infection substantially reduced the incidence of some diseases associated with opportunistic viral infections. however, the incidence of ain is reported to increase and haart seems to have only little impact on the regression or progression of ain. paradoxically, improvement of survival in the haart era results in an increa ... | 2003 | 14502448 |
| prostate cancer risk and serologic evidence of human papilloma virus infection: a population-based case-control study. | epidemiological evidence is accumulating that sexual history may be associated with prostate cancer, and some studies have suggested a relation between human papilloma virus (hpv) infections and prostate cancer. we measured the presence of antibodies to the major oncogenic hpv types 16, 18, and 33 among 238 subjects with untreated prostate cancer and 210 population-based control subjects. odds ratios (ors) were estimated from multivariate logistic regression models, controlling for age and hpv t ... | 2003 | 14504197 |
| lifestyle and cancer: effect of widowhood and divorce. | limited data are available on the possible changes in cancer risk brought about by widowhood and divorce, an increasing segment of the population. we calculated standardized incidence ratios (sirs) for cancer among 47,000 widows/widowers and 60,000 divorced people, based on the swedish family-cancer database. persons had to be identified with the same civil status in the census of years 1960 and 1970; the comparison group was married people according to the same censuses. cancers were followed f ... | 2003 | 14504201 |
| safety and immunogenicity of a peptide containing the cross-neutralization epitope of hpv16 l2 administered nasally in healthy volunteers. | amino acid (aa) 108-120 of l2 protein of human papillomavirus (hpv) type 16 contains a cross-neutralization epitope against genital hpv. we designed a placebo-controlled trial in healthy adults to evaluate the safety and immunogenicity of a synthetic peptide consisting of the aa 108-120 of hpv16 l2 (l2-108/120) region. a total of 13 volunteers were given nasal inoculations with 0.1 (n=5) or 0.5mg (n=5) doses of the peptides or placebo (n=3) without adjuvant at weeks 0, 4, and 12. sera were colle ... | 2003 | 14505907 |
| a subset of head and neck squamous cell carcinomas exhibits integration of hpv 16/18 dna and overexpression of p16ink4a and p53 in the absence of mutations in p53 exons 5-8. | besides well-known risk factors such as tobacco use and alcohol consumption, oncogenic human papillomavirus (hpv) infection also has recently been suggested to promote head and neck tumorigenesis. hpv is known to cause cancer by inactivation of cell cycle regulators p53 and prb via expression of viral oncoproteins e6 and e7. this indicates that p53 mutations are not a prerequisite in hpv-induced tumor development. however, discrepancy exists with respect to the frequency of head and neck squamou ... | 2003 | 14506739 |
| prevalence and physical status of human papillomavirus in squamous cell carcinomas of the head and neck. | fresh-frozen biopsies were obtained from 61 patients at diagnosis of squamous cell carcinoma of the head and neck (hnscc) for study of the prevalence and physical status of human papillomavirus (hpv) dna. the frequency of hpv dna and genotypes were determined by spf10 pcr screening with a general probe hybridization and inno-lipa hpv genotyping assay. in addition, a single-phase pcr with primers fap 59/64 and a nested pcr with primers cp 65/70 and cp 66/69 served to detect particularly cutaneous ... | 2003 | 14506740 |
| human papillomavirus type 16 and 18 l1 protein peptide binding to vero and hela cells inhibits their vlps binding. | human papillomaviruses (hpvs) are the cause of epithelial lesions, hpv type 16 and type 18 being associated with the development of anogenital cancer. the l1 major capsid protein (l1) represents about 90% of total hpv protein and is involved in virus-host cell interaction, but little is known about this binding process. l1 sequences from hpv types 16 and 18 were synthesized in 56 20-mer peptides, covering the entire protein, hplc-purified, (125)i-radiolabeled and tested in vero and hela cell-bin ... | 2003 | 14506742 |
| genetic analysis of cutaneous squamous cell carcinomas arising from different areas. | although cutaneous squamous cell carcinomas (scc) occur most frequently in sun-exposed areas of the skin, they can also arise in non-sun-exposed areas. some risk factors for cutaneous scc, such as ultraviolet (uv) light, are well known. however, the major factor for carcinogenesis may depend on the site of the tumor as well as the ethnicity of the patient. in this study we examined 41 japanese cutaneous scc cases, focusing on the area of appearance and the presence of genetic alteration, with 27 ... | 2003 | 14507317 |
| augmentation of dna vaccine potency through secretory heat shock protein-mediated antigen targeting. | in an effort to enhance the potency of dna vaccines, we have developed a new strategy to increase antigen presentation by dendritic cells, one that results in markedly improved cytotoxic t-lymphocyte responses, antibody production, and antitumor effects in vivo. here, we present the rationale and design of a vaccine encoding a secreted antigen-heat shock protein 70 fusion molecule, targeted to the mhc class i cross-presentation pathway of dendritic cells. using the human papilloma virus 16 e7 pr ... | 2003 | 14511955 |
| human papillomavirus and grzybowski's generalized eruptive keratoacanthoma. | 2003 | 14512942 | |
| differential deletions of chromosome 3p are associated with the development of uterine cervical carcinoma in indian patients. | deletions in chromosome 3 occur frequently in uterine cervical carcinoma (ca-cx). the common consensus regions deleted during ca-cx development are not well defined, and have not been correlated with tumour progression. | 2003 | 14514919 |
| dissection of human papillomavirus type 33 l2 domains involved in nuclear domains (nd) 10 homing and reorganization. | we have recently shown that the minor capsid protein l2 of human papillomavirus type 33 (hpv33) recruits the transcriptional repressor daxx into nuclear domains (nd) 10 and causes the loss of the transcriptional activator sp100 from these subnuclear structures. in order to dissect l2 domains involved in nuclear translocation, nd10 homing, loss of sp100, and recruitment of daxx, a detailed deletion mutagenesis of l2 was performed. using immunofluorescence and green fluorescent protein fusions, we ... | 2003 | 14517069 |
| the adeno-associated virus major regulatory protein rep78-c-jun-dna motif complex modulates ap-1 activity. | multiple epidemiologic studies show that adeno-associated virus (aav) is negatively associated with cervical cancer (cx ca), a cancer which is positively associated with human papillomavirus (hpv) infection. mechanisms for this correlation may be by rep78's (aav's major regulatory protein) ability to bind the hpv-16 p97 promoter dna and inhibit transcription, to bind and interfere with the functions of the e7 oncoprotein of hpv-16, and to bind a variety of hpv-important cellular transcription fa ... | 2003 | 14517094 |
| immunological and clinical responses in women with vulval intraepithelial neoplasia vaccinated with a vaccinia virus encoding human papillomavirus 16/18 oncoproteins. | this study assessed the immunological and clinical responses of women with human papillomavirus (hpv) 16-associated high-grade vulval intraepithelial neoplasia (vin) vaccinated with ta-hpv, a recombinant vaccinia virus encoding modified hpv 16 and 18 e6 and e7. eighteen women with hpv 16-positive high-grade vin were vaccinated with ta-hpv. the extent of their baseline disease was compared after 24 weeks by lesion measurements and histological analysis. viral load was assessed pre- and postvaccin ... | 2003 | 14522932 |
| hpv testing can reduce the number of follow-up visits in women treated for cervical intraepithelial neoplasia grade 3. | we evaluated high-risk human papillomavirus (hpv) testing by hybrid capture ii (hc ii) in addition to cytology to predict recurrent/residual cervical intraepithelial neoplasia (cin) 2/3 and cervical cancer in women treated for cin 3. | 2003 | 14529664 |
| boosting with recombinant vaccinia increases hpv-16 e7-specific t cell precursor frequencies and antitumor effects of hpv-16 e7-expressing sindbis virus replicon particles. | immunotherapy using the heterologous prime-boost regimen has emerged as an attractive approach for generating antigen-specific t-cell-mediated immune responses against tumors and infectious diseases. we have previously linked the mycobacterium tuberculosis heat-shock protein 70 (hsp70) to the hpv-16 e7 antigen creating a chimera, e7/hsp70. we found that nucleic acid vaccines encoding e7/hsp70 can generate strong antitumor immunity. recently, replication-defective sindbis virus replicon particle ... | 2003 | 14529828 |
| enhanced effector and memory ctl responses generated by incorporation of receptor activator of nf-kappa b (rank)/rank ligand costimulatory molecules into dendritic cell immunogens expressing a human tumor-specific antigen. | the outcome of dendritic cell (dc) presentation of ag to t cells via the tcr/mhc synapse is determined by second signaling through cd80/86 and, importantly, by ligation of costimulatory ligands and receptors located at the dc and t cell surfaces. downstream signaling triggered by costimulatory molecule ligation results in reciprocal dc and t cell activation and survival, which predisposes to enhanced t cell-mediated immune responses. in this study, we used adenoviral vectors to express a model t ... | 2003 | 14530334 |
| human papillomavirus in a 16th century mummy. | 2003 | 14550719 | |
| lower airway papillomatosis in children. | laryngeal papilloma in children is a frequent disease caused by human papilloma virus (hpv) type 6 or type 11. this disease has a tendency to recur and the changes are histologically benign. in some cases papilloma may affect the lower levels of the respiratory tract. in this study, among 90 patients treated for laryngeal papillomatosis, in four children papilloma of trachea, bronchi and lung tissue were detected in endoscopic and radiological examination. this constitutes 4.4% of all patients. ... | 2003 | 14550967 |
| differential deletions in 3p are associated with the development of head and neck squamous cell carcinoma in indian patients. | in this study we performed detailed deletion mapping of two broad regions in the short arm (p) of chromosome 3 (i.e., 3p21.2 approximately p22 and 3p12 approximately p13), which were shown to have a high rate of deletions in head and neck lesions in our previous study. using 18 highly polymorphic microsatellite markers, the deletion mapping was done in 35 dysplastic lesions and 46 primary head and neck squamous cell carcinoma (hnscc) samples from indian patients. within the 21.6-megabase (mb) re ... | 2003 | 14553947 |
| human papillomavirus type 59 immortalized keratinocytes express late viral proteins and infectious virus after calcium stimulation. | human papillomavirus type 59 (hpv 59) is an oncogenic type related to hpv 18. hpv 59 was recently propagated in the athymic mouse xenograft system. a continuous keratinocyte cell line infected with hpv 59 was created from a foreskin xenograft grown in an athymic mouse. cells were cultured beyond passage 50. the cells were highly pleomorphic, containing numerous abnormally shaped nuclei and mitotic figures. hpv 59 sequences were detected in the cells by dna in situ hybridization in a diffuse nucl ... | 2003 | 14554084 |
| identification of a human papillomavirus type 16-specific epitope on the c-terminal arm of the major capsid protein l1. | to characterize epitopes on human papillomavirus (hpv) virus-like particles (vlps), a panel of mutated hpv-16 vlps was created. each mutated vlp had residues substituted from hpv-31 or hpv-52 l1 sequences to the hpv-16 l1 backbone. mutations were created on the hpv-31 and -52 l1 proteins to determine if hpv-16 type-specific recognition could be transferred. correct folding of the mutated proteins was verified by resistance to trypsin digestion and by binding to one or more conformation-dependent ... | 2003 | 14557648 |
| [value of acetic acid smear test for cervical cancer screening]. | at present, the incidence of cervical cancer is still high in less developed areas of china; one of the reasons is that cytology in screening for cervical cancer is not feasible in these areas. the aim of this study was to analyze the sensitivity and specificity of acetic acid smear test as a cervical cancer screening test, and evaluate the value of this method for cervical cancer screening. | 2003 | 14558960 |
| the fidelity of hpv16 e1/e2-mediated dna replication. | human papillomaviruses (hpv) are causative agents in a variety of human diseases; for example over 99% of cervical carcinomas contain hpv dna sequences. often in cervical carcinoma the hpv genome is integrated into the host genome resulting in unregulated expression of the viral transforming proteins e6 and e7. therefore viral integration is a step toward hpv-induced carcinogenesis. integration of the hpv genome could occur following double-strand dna breaks that could arise during viral dna rep ... | 2003 | 14559922 |
| efficiency of the hybrid capture 2 hpv dna test in cervical cancer screening. a study by the french society of clinical cytology. | the aim of this study was to determine the efficiency of the hybrid capture 2 (hc2; digene, gaithersburg, md) human papillomavirus (hpv) assay for the detection of cervical neoplasia. of the 1,785 patients recruited, 462 (25.88%) were referred for colposcopy owing to previously detected cytologic abnormalities, and 1,323 (74.12%) were voluntary candidates for screening. for all patients, a papanicolaou smear and a monolayer smear (thinprep, cytyc, boxborough, ma) were done. hpv dna was detected ... | 2003 | 14560561 |
| preferential integration of human papillomavirus type 18 near the c-myc locus in cervical carcinoma. | the development of cervical cancer is highly associated with human papillomavirus (hpv) infection. greater than 99% of all cervical tumors contain hpv dna. integration of high-risk hpv has been temporally associated with the acquisition of a malignant phenotype. recent work from our lab has shown that hpv16, the most common high-risk hpv associated with cervical carcinoma, preferentially integrates at loci containing human common fragile sites (cfss). cfss are regions of genomic instability that ... | 2003 | 14562053 |
| monoclonal expansion with integration of high-risk type human papillomaviruses is an initial step for cervical carcinogenesis: association of clonal status and human papillomavirus infection with clinical outcome in cervical intraepithelial neoplasia. | to define the natural history of cervical intraepithelial neoplasia (cin) as related to clonal status, we evaluated 20 cases of cin1 and 18 cases of cin2 that had been clinically followed for 7 to 48 months at osaka university hospital. these included 10 cases that progressed, 15 cases that persisted, and 13 cases that regressed. we analyzed the clonal status of each case by analysis of the pattern of x-chromosomal inactivation. human papillomavirus (hpv) infection was detected by pcr-rflp analy ... | 2003 | 14563953 |
| human papillomavirus infection of the epididymis and ductus deferens: an evaluation by nested polymerase chain reaction. | human papillomaviruses (hpvs) play an important role in the etiology of squamous cell carcinoma of the uterine cervix. the possible role of the male urogenital tract as a reservoir of hpv infection is not fully understood. we inferred from our previous observation of hpv-31 in epididymal tissue in a case of chronic epididymitis that hpv might be commonly present in cases of epididymitis caused by sexually transmitted pathogens. | 2003 | 14567721 |
| epstein-barr virus detected in a head and neck squamous cell carcinoma cell line derived from an immunocompromised patient. | dna tumor viruses potentially play a role in the development of squamous cell carcinoma of the head and neck (scchn). human papillomavirus is found in up to 50% of scchn specimens, and epstein-barr virus (ebv) has been detected in nasopharyngeal carcinoma, burkitt lymphoma, and other lymphomas. however, the role of ebv in nonnasopharyngeal scchn has not been thoroughly investigated. | 2003 | 14568799 |
| il-10 mediates suppression of the cd8 t cell ifn-gamma response to a novel viral epitope in a primed host. | priming to ag can inhibit subsequent induction of an immune response to a new epitope incorporated into that ag, a phenomenon referred to as original antigenic sin. in this study, we show that prior immunity to a virus capsid can inhibit subsequent induction of the ifn-gamma effector t cell response to a novel cd8-restricted antigenic epitope associated with the virus capsid. inhibition does not involve ab to the virus capsid, as it is observed in animals lacking b cells. cd8-restricted virus-sp ... | 2003 | 14568953 |
| increased expression of the huntingtin interacting protein-1 gene in cells from hutchinson gilford syndrome (progeria) patients and aged donors. | hutchinson gilford syndrome (progeria [pg]) is a human disease associated with accelerated aging. to elucidate the acceleration mechanism, we first tried to transform a pg-derived cell line by infection of a recombinant adenovirus expressing hpv (human papilloma virus)-e6 and hpv-e7 genes. the transfected pg cells had a greater number of population doublings (pd) (>80), faster doubling time, and less staining of senescence-associated ss-galactosidase than the nontransfected pg cells. the transfe ... | 2003 | 14570852 |
| [contribution of high risk human papillomavirus testing to the management of premalignant and malignant lesions of the uterine cervix]. | high risk human papillomaviruses (hr-hpv) are consistently associated with premalignant and malignant lesions of the uterine cervix. thus, the use of molecular techniques to detect hpv has been proposed to improve the results of conventional diagnostic strategies. in the present study, we evaluated the usefulness of the detection of hr-hpv in a cervical pathology unit. | 2003 | 14572367 |
| identification and characterization of a cluster of transcription start sites located in the e6 orf of human papillomavirus type 16. | human papillomavirus type 16 (hpv-16) is the prototype strain among the malignant types of hpv in the western world. the main promoter, p97, located in front of the e6 orf, has been shown to control expression of the oncogenes e6 and e7. these oncogenes are expressed continuously in hpv-16-transformed cells. in contrast to malignant hpv types, non-malignant hpv types have separate promoters driving the expression of e6 and e7. experiments have shown that the translation of e7 is more efficient f ... | 2003 | 14573795 |
| the regulatory e2 proteins of human genital papillomaviruses are pro-apoptotic. | cervical carcinomas are frequently associated with infection by human papillomaviruses (hpvs). these viruses encode two oncogenes e6 and e7, which promote cell proliferation and immortalization. the viral e2 protein represses transcription of the e6/e7 oncogenes and activates viral dna replication together with the viral e1 helicase. the e2 protein is specifically inactivated in hpv18-associated carcinoma, suggesting that it may prevent carcinogenic progression. indeed, e2 was shown to exhibit a ... | 2003 | 14585548 |
| re: "genital human papillomavirus infection: incidence and risk factors in a cohort of female university students". | 2003 | 14585772 | |
| toward a reduction of the global burden of cervical cancer. | of the 190,000 deaths from cervical cancer that occur annually worldwide, the majority take place in developing countries. recent advances in our understanding of the causes and natural history of cervical neoplasia and, in particular, the establishment of the central role of human papilloma virus (hpv) infection have created opportunities for the primary and secondary prevention of cervical cancer. in the future, prevention efforts will include the incorporation of hpv testing as an adjunct to ... | 2003 | 14586318 |
| replication and interaction of herpes simplex virus and human papillomavirus in differentiating host epithelial tissue. | we have investigated the interactions and consequences of superinfecting and coreplication of human papillomavirus (hpv) and herpes simplex virus (hsv) in human epithelial organotypic (raft) culture tissues. in hpv-positive tissues, hsv infection and replication induced significant cytopathic effects (cpe), but the tissues were able to recover and maintain a certain degree of tissue integrity and architecture. hpv31b not only maintained the episomal state of its genomic dna but also maintained i ... | 2003 | 14592758 |
| the dna-binding domain of human papillomavirus type 18 e1. crystal structure, dimerization, and dna binding. | high risk types of human papillomavirus, such as type 18 (hpv-18), cause cervical carcinoma, one of the most frequent causes of cancer death in women worldwide. dna replication is one of the central processes in viral maintenance, and the machinery involved is an excellent target for the design of antiviral therapy. the papillomaviral dna replication initiation protein e1 has origin recognition and atp-dependent dna melting and helicase activities, and it consists of a dna-binding domain and an ... | 2004 | 14593106 |
| in vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by e6 sirna. | human papillomavirus type 16 (hpv16), a causative agent of cervical cancers, encodes the e6 and e7 oncogenes, whose simultaneous expression is pivotal for malignant transformation and maintenance of malignant phenotypes. in the hope of developing a gene-specific therapy for hpv-related cancer, we examined the effects of e6 short-interfering rna (sirna) on the expression of these oncogenes and on the cell growth of hpv16-related cervical cancer cells. using siha cervical cancer cells, we demonstr ... | 2003 | 14599809 |
| in vitro downregulation of growth factors by insulin-like growth factor binding protein-3 in cervical cancer. | our hypothesis is that insulin-like growth factor binding protein 3 (igf-bp3) would downregulate epidermal growth factor receptor (egf-r) levels in cervical cancer cell lines, thereby reducing cellular igf-ii and angiogenesis-related vascular endothelial cell growth factor (vegf). as folate deficiency is a risk factor in cervical cancer, we sought to determine if folic acid treatment might increase igf-bp3 production, thereby inhibiting malignant cell proliferation. | 2003 | 14599874 |
| innovations in understanding the biology of cervical cancer. | revelation of the connection between the human papillomavirus (hpv) and cervical neoplasia and invasive cervical cancer is prompting new investigations to expand that understanding and promote vaccines, gene therapy, and other interventions. at the second international conference on cervical cancer (houston, tx, april 11-14, 2002), laboratory and clinical researchers reported advances in new studies meant to increase understanding of the natural history of hpv and cervical intraepithelial neopla ... | 2003 | 14603543 |
| distribution of human papillomavirus types 16 and 18 variants in squamous cell carcinomas and adenocarcinomas of the cervix. | the distributions of human papillomavirus (hpv) types detected in cervical adenocarcinomas and squamous cell tumors differ. however, whether the distributions of intratypic hpv variants seen in these two histological forms of cervical disease differ is unknown. our objective was to compare the distribution of hpv intratypic variants observed in squamous cell carcinomas (scc) and cervical tumors of glandular origin (e.g., adenocarcinomas; ac) for two hpv types commonly observed in cervical tumors ... | 2003 | 14612516 |
| the prevalence of human papillomavirus genotypes in nonmelanoma skin cancers of nonimmunosuppressed individuals identifies high-risk genital types as possible risk factors. | nonmelanoma skin cancer is the most commonly diagnosed malignant disease in caucasians. known risk factors include fair skin, sun exposure, male gender, advancing age, and the presence of solar keratosis. no viral risk factors have been established thus far. to examine the association between nonmelanoma skin cancer and infection with human papilloma virus (hpv) types, we performed a retrospective study in which skin biopsies were collected from 496 nonimmunosuppressed patients attending dermato ... | 2003 | 14612553 |
| extended duration of the detectable stage by adding hpv test in cervical cancer screening. | the human papillomavirus test (hpv) test could improve the (cost-) effectiveness of cervical screening by selecting women with a very low risk for cervical cancer during a long period. an analysis of a longitudinal study suggests that women with a negative pap smear and a negative hpv test have a strongly reduced risk of developing cervical abnormalities in the years following the test, and that hpv testing lengthens the detectable stage by 2-5 years, compared to pap smear detection alone. | 2003 | 14612887 |
| cervical cytology screening in teens. | new guidelines for when to initiate cervical cancer screening have recently been revised. the american cancer society now recommends that screening be initiated within 3 years of the onset of vaginal intercourse but no later than 21 years of age. natural history studies of human papillomavirus (hpv; the cause of abnormal cytology and cervical cancer) suggest that there is little risk of a significant precancerous lesion going undetected within the first 3 to 5 years after the onset of sexual act ... | 2003 | 14613663 |
| vaccinia-expressed human papillomavirus 16 and 18 e6 and e7 as a therapeutic vaccination for vulval and vaginal intraepithelial neoplasia. | anogenital intraepithelial neoplasia is a chronic disorder associated with infection by high-risk human papillomavirus (hpv) types. it is frequently multifocal and recurrence after conventional treatment is high. boosting hpv-specific cell-mediated immune responses may reduce progression to carcinoma and could lead to disease clearance. we have tested the safety, immunogenicity, and efficacy of a recombinant vaccinia candidate vaccine (ta-hpv) in women with anogenital intraepithelial neoplasia. | 2003 | 14614000 |
| peptides inhibitory for the transcriptional regulatory function of human papillomavirus e2. | human papillomavirus (hpv) infections are associated with cervical neoplasia. cellular and viral proteins are known to interact with the papillomavirus e2 protein to initiate transcription and dna replication in the hpv life cycle. our aim was to identify peptides that bind to the hpv16 e2 protein and thereby inhibit its ability to alter the transcriptional activity of other genes. | 2003 | 14614029 |
| detection and genotyping of human papillomavirus dna in cervical cancer tissues with fluorescence polarization. | to evaluate the type-specific prevalence of eight common types of human papillomavirus (hpv) in patients with cervical cancer living in shanxi, china, with fluorescence polarization detection, crude dna extracted from 137 samples of early-stage cervical cancer (within stage iia) and chronic cervicitis was subjected to hpv l1 consensus gp5+/gp6+ system. then, the hpv-positive products identified by gp5 + /gp6+ pcr were genotyped based on template-directed dye-terminator incorporation assay with f ... | 2003 | 14614542 |
| microsatellite analysis of early stage (ia-iib) uterine cervical squamous carcinoma. | cervical cancer is the most common gynecologic malignancy of the developing world. the oncogenic role of human papilloma virus (hpv) is well known. attention is now focusing on the complicit genetic changes, which allow progression of these tumors. regarding these changes, deletion of tumor suppressor genes (loss of heterozygosity [loh]) is the preferred pathway of progression with only a subset manifesting microsatellite instability (msi). implicated loci include 3p14.1-22. several studies sugg ... | 2003 | 14615820 |
| viral warts in organ transplant recipients: new aspects in therapy. | the long-term success of organ transplantation depends on the prevention of allograft rejection and improvement in quality of life for the patients. this has been achieved through better immunosuppressive regimens with lower dosages and a new generation of immunosuppressive drugs. however, these immunosuppressive agents not only impair the patient's reactivity to the graft, but also to infectious organisms, thereby making them more susceptible to opportunistic pathogens. because of this, organ t ... | 2003 | 14616341 |
| tumor necrosis factor-related apoptosis-inducing ligand (trail) decoy receptor trail-r3 is up-regulated by p53 in breast tumor cells through a mechanism involving an intronic p53-binding site. | tumor necrosis factor-related apoptosis-inducing ligand receptor 3 (trail-r3) is a decoy receptor for trail, a member of the tumor necrosis factor family. in several cell types decoy receptors inhibit trail-induced apoptosis by binding trail and thus preventing its binding to proapoptotic trail receptors. we studied the regulation of trail-r3 gene expression in breast tumor cells treated with the genotoxic drug doxorubicin (dxr). the breast tumor cell line mcf-7 (p53 wild type) responded to dxr ... | 2004 | 14623878 |
| rna interference: a potential tool against kaposi's sarcoma-associated herpesvirus. | rna interference is a conserved cellular function that controls viral infection, the expression of transposable elements, repetitive sequences and genes in embryonic development. originally described as an antiviral mechanism in plants, known as posttranscriptional gene silencing, it is now appreciated that this phenomenon occurs in all living cells. double-stranded rna, when acting as part of rna interference, reduces expression of genes with sequence similarity, but has no effect on the expres ... | 2003 | 14624111 |
| molecular analysis of human papillomavirus type 52 isolates detected in the genital tract of human immunodeficiency virus-seropositive and -seronegative women. | human papillomavirus (hpv) type 52 dna was detected in cervicovaginal lavage samples from 91 (12.4%) of 732 human immunodeficiency virus (hiv)-seropositive women and 23 (7.1%) of 323 hiv-seronegative women (p=.0004). hiv infection was an independent predictor for hpv-52 infection when controlling for age and sexual activity (odds ratio, 2.21; 95% confidence interval, 1.30-3.75: p=.003). we describe the genomic polymorphism of 114 hpv-52 isolates. long control region (lcr) mutations defined 27 hp ... | 2003 | 14624377 |
| successful immortalization of endometrial glandular cells with normal structural and functional characteristics. | the human endometrium is a dynamic tissue, the proliferative activity of which dramatically changes throughout the menstrual cycle, with exquisite regulation by sex-steroid hormones. primary endometrial epithelial cells fall into senescence within 2 weeks when cultured on plastic dishes, and more complete understanding of endometrial biology has been delayed because of, in part, a lack of an in vitro culture model for endometrial epithelial cells. our goal was to establish immortalized human end ... | 2003 | 14633600 |
| an alpha helix conformationally restricted peptide is recognized by cervical carcinoma patients' sera. | human papillomavirus type 16 (hpv-16) represents the major cervical carcinoma associated virus among women, especially in colombia. it has thus become important to develop reliable inexpensive tests for detecting the presence of this virus. it has been shown that hpv16-e7 oncoprotein structural features have three alpha-helical structures and a loop-like structure. the hydrazone link approach was used to mimic helix secondary substructures. sera from women with invasive cervical carcinoma were t ... | 2003 | 14640547 |
| optical systems for in vivo molecular imaging of cancer. | progress toward a molecular characterization of cancer would have important clinical benefits; thus, there is an important need to image the molecular features of cancer in vivo. in this paper, we describe a comprehensive strategy to develop inexpensive, rugged and portable optical imaging systems for molecular imaging of cancer, which couples the development of optically active contrast agents with advances in functional genomics of cancer. we describe initial results obtained using optically a ... | 2003 | 14640761 |
| association between esophageal squamous cell carcinoma and human papillomavirus detected by hybrid capture ii assay. | from july 1999 to july 2000, 40 patients from the surgical oncology and gastroenterology departments of irmandade da santa casa de misericórdia de porto alegre were enrolled in a prospective study in order to find the frequency of human papillomavirus dna (hpv-dna) in esophageal squamous cell carcinoma. they were compared to 10 normal individuals (controls). digene inc. (digene corporation, gaithersburg md, usa) hybrid capture ii assay, a chemoluminescent method, was used in both groups. the sam ... | 2003 | 14641314 |
| human papillomavirus dna in sera of cervical cancer patients as tumor marker. | persistent infection with human papillomavirus (hpv) has been widely recognized to induce carcinoma of the uterine cervix. viral dna has been documented to occur as tumor dna in the circulation of patients with primary tumors caused by viral infection. the aim of this study was to evaluate the utility of serum hpv dna as tumor marker in cervical cancer patients. sera taken from 94 cervical cancer patients at the date of diagnosis and follow-up serum samples from 24 patients were examined for hpv ... | 2003 | 14643453 |
| genetic susceptibility to infection with human papillomavirus and development of cervical cancer in women in brazil. | human papillomavirus (hpv) is considered to be a necessary but not sufficient cause for cervical cancer and, therefore, other factors contribute to the carcinogenic process. a hereditary component for this neoplasia has been reported and several studies indicate that genetic background of the host is important for cervical cancer susceptibility. among genetic factors that could participate in the susceptibility to this tumor and disease outcome, polymorphic genes of the major histocompatibility ... | 2003 | 14644340 |
| infection, replication, and cytopathology of human papillomavirus type 31 in trophoblasts. | human papillomavirus (hpv) dna is preferentially found in spontaneous abortions, specifically residing in trophoblasts, and transfected hpv-16 dna replicates and produces progeny in 3a trophoblasts in culture. in this study 3a trophoblasts were shown to display both hpv receptors and infection by hpv-31b and hpv-6 virus resulted in de novo (increasing) hpv dna replication in these cells (inhibited by neutralizing anti-hpv31b antibodies). reverse transcription-polymerase chain reaction analysis r ... | 2003 | 14644610 |
| an adenoviral vector cancer vaccine that delivers a tumor-associated antigen/cd40-ligand fusion protein to dendritic cells. | to develop a method to overcome the anergy that exists in tumor hosts to cancer, we have designed an adenoviral vector for the in vivo activation and tumor antigen loading of dendritic cells. this adenoviral vector encodes a fusion protein composed of an amino-terminal tumor-associated antigen fragment fused to the cd40 ligand (cd40l). subcutaneous injection of an adenoviral vector encoding a fusion protein of the human papillomavirus e7 foreign antigen linked to the cd40l generates cd8+ t cell- ... | 2003 | 14645711 |
| socioeconomic factors and cervical cancer mortality in spain during the period 1989-1997. | a study was made of cervical cancer (cc) mortality trends in spain during the period 1989-1997 at national, autonomous community and provincial levels, in relation to different socioeconomic factors. data were obtained from the spanish national institute of statistics (instituto nacional de estadística, ine). the crude mortality rates were age-adjusted using the indirect method and gross domestic product (gdp) as socioeconomic status (ses) indicator. | 2004 | 14648177 |
| cervical cancer control, priorities and new directions. | cervical cancer is caused by infection with a range of high risk "oncogenic" human papillomavirus (hpv) types, and it is now accepted that >99% of cervical cancer is initiated by hpv infection. the estimated lifetime risk of cervical cancer is nevertheless relatively low (less than 1 in 20 for most community based studies). although sensitivity and specificity of the available diagnostic techniques are suboptimal, screening for persistent hpv infection is effective in reducing the incidence of c ... | 2004 | 14648697 |
| human papillomavirus and oral cancer: the international agency for research on cancer multicenter study. | human papillomavirus (hpv), the causal agent of cervical cancer, appears to be involved in the etiology of cancer of the oral cavity and oropharynx. to investigate these associations, we conducted a multicenter case-control study of cancer of the oral cavity and oropharynx in nine countries. | 2003 | 14652239 |
| prevalence and determinants of chlamydia trachomatis infections in women from bogota, colombia. | chlamydia trachomatis infection in the cervix and uterus has been hypothesised to be a co-factor for cervical cancer. we performed a cross sectional study in bogota, colombia, where cervical cancer rates are high, to determine the prevalence and determinants of c trachomatis infection, and in particular its association with human papillomavirus (hpv). | 2003 | 14663124 |
| high-risk human papillomaviruses may have an important role in non-oral habits-associated oral squamous cell carcinomas in taiwan. | to evaluate the etiologic role of human papillomavirus (hpv) in oral carcinogenesis, dna samples were purified from 103 oral squamous cell carcinoma (oscc) and 30 normal oral mucosal (nom) specimens. a nested polymerase chain reaction, dna sequencing, and gene-chip hpv typing were used to identify multiple hpv types in our samples. we found that the positive rates of all hpv types and of high-risk hpv types were significantly higher in oscc samples (49.5% and 41.7%, respectively) than in nom sam ... | 2003 | 14671980 |
| serum immunoglobulin a response to human papillomavirus type 16 virus-like particles in human immunodeficiency virus (hiv)-positive and high-risk hiv-negative women. | serum samples from 2008 human immunodeficiency virus (hiv)-positive and 551 hiv-negative women were tested for immunoglobulin a (iga) to human papillomavirus (hpv) type 16 capsids. iga seropositivity was lower than previously reported igg seropositivity (7% vs. 51%), but, like igg antibodies, hpv 16 iga was associated with sexual behavior, cervicovaginal hpv 16 dna, and cytological abnormalities. iga seropositivity was higher in hiv-positive women than in hiv-negative women (7.7% vs. 4.9%; p=.02 ... | 2003 | 14673762 |
| presence of human papillomavirus dna in plucked eyebrow hairs is associated with a history of cutaneous squamous cell carcinoma. | a role for cutaneous human papillomaviruses (hpv) has been proposed in the development of skin cancer. well-designed epidemiologic studies to demonstrate an association between hpv infection and skin cancer are extremely rare. to identify hpv infection as a potential risk factor, we investigated the association between the presence of hpv dna in eyebrow hairs and a history of cutaneous squamous cell carcinoma. a case-control study was designed consisting of 155 immunocompetent individuals with a ... | 2003 | 14675206 |
| the human papillomavirus type 11 and 16 e6 proteins modulate the cell-cycle regulator and transcription cofactor trip-br1. | the genital human papillomaviruses (hpvs) are a taxonomic group including hpv types that preferentially cause genital and laryngeal warts ("low-risk types"), such as hpv-6 and hpv-11, or cancer of the cervix and its precursor lesions ("high-risk types"), such as hpv-16. the transforming processes induced by these viruses depend on the proteins e5, e6, and e7. among these oncoproteins, the e6 protein stands out because it supports a particularly large number of functions and interactions with cel ... | 2003 | 14675634 |
| prevention of cervical cancer in africa: a daunting task? | africa has a high estimated incidence of cervical cancer, thus requiring the development of an effective prevention strategy. cytology-based screening is beyond the capacity of many african countries, hence the need for alternatives. visual inspection of the cervix after application of 3-5% acetic acid (via) is a promising screening test, with similar sensitivity to that of cytology but lower specificity. the same accounts for other via methods using magnification devices, visual inspection afte ... | 2003 | 14677294 |
| cervical human papillomavirus infections in commercial sex workers-risk factors and behaviours. | we have investigated the prevalence of, and risk factors for, cervical human papillomavirus (hpv) infection in commercial sex workers (csws) and controls attending the same sexual health clinics in sydney. a self-administered 'risk factor' questionnaire was completed and a pap smear and a specimen for hpv detection and typing were taken. results from the 288 csws and 266 controls were assessed by univariate and multivariate analyses. no significant difference in the rates of cervical hpv infecti ... | 2003 | 14678594 |
| digene corporation. | digene corporation is a molecular diagnostics company that develops, manufactures and markets proprietary gene-based testing systems for the screening, monitoring and diagnosis of human diseases. the company's primary focus is in women's cancers and infectious diseases. our proprietary, patented hybrid capture((r)) technology has been successfully applied to the development of diagnostic tests for human papillomavirus (hpv), chlamydia, gonorrhea, hepatitis b virus and cytomegalovirus. digene's h ... | 2004 | 14683424 |
| [identification and typification of the human papilloma virus in women using the "timely detection of cancer" program in durango, mexico]. | in mexico, the cervical carcinoma is a public health problem, representing more of 36% of the neoplasms. this carcinoma has been etiologically associated with the human papillomavirus (hpv) infection and the polymerase chain reaction (pcr) has been considered as one of the most sensitive methods for its detection. the aim of this study was to determine the hpv types by pcr-rflps in 111 women attending in the doc programs in gomez palacio, durango. | 2003 | 14686060 |
| the epidemiology of cervical cancer. | cervical cancer is one of the most common neoplastic diseases affecting women, with a combined worldwide incidence of almost half a million new cases annually, second only to breast cancer. basic and epidemiologic research conducted during the past 15-20 years have provided overwhelming evidence for an etiologic role for infection with certain types of sexually-transmitted human papillomavirus (hpv) as the primary cause of cervical cancer. the relative risks of cervical cancer following hpv infe ... | 2003 | 14690309 |