Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| skin-test reactivity and isotype-specific immune responses to recombinant asp f 3, a major allergen of aspergillus fumigatus. | the in vitro diagnostic value of recombinant allergens depends on the correlation between allergen specific ige in serum and ability of the protein to elicit immediate type allergic reactions in sensitized individuals. objective the study was carried out to evaluate the reliability of recombinant asp f 3 (rasp f 3)-based serological determinations in elisa and immunocap compared to skin-test responses in a. fumigatus sensitized subjects. | 1998 | 9720820 |
| cytokine- and t-helper-dependent immunity in murine aspergillosis. | 1998 | 9720962 | |
| pulmonary host defences against aspergillus fumigatus. | 1998 | 9720963 | |
| cytokines in allergic bronchopulmonary aspergillosis. | 1998 | 9720964 | |
| microbiological profile and potential hazards associated with imported and local brands of tomato paste in nigeria. | cans of three tomato paste brands (two of which are imported and one produced locally) showing defective or normal appearance were purchased from various retail outlets and analysed for microbial composition and ph values. substantially higher total viable counts were observed in samples from defective cans but the lowest population was found in the local brand. ratio of mesophilic to thermophilic micro-organisms increased in samples obtained from cans showing visible defects. anaerobic spore co ... | 1998 | 9721645 |
| disease-specific recombinant allergens for the diagnosis of allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa), a severe pulmonary complication caused by aspergillus fumigatus, is considered a complex clinical syndrome with defined serological, pathological radiological and clinical features. the diagnosis of abpa is often difficult because of several overlapping clinical and laboratory findings shared between asthma with sensitization to a. fumigatus and abpa, but essential for treatment to prevent severe deterioration of pulmonary function. we have cloned ... | 1998 | 9723708 |
| acute community-acquired pneumonia due to aspergillus in presumably immunocompetent hosts: clues for recognition of a rare but fatal disease. | this article reports a case of acute community-acquired pneumonia due to aspergillus fumigatus in a healthy patient and reviews 11 previously reported cases occurring in presumably immunocompetent hosts. the diagnosis was delayed for all patients; mortality was 100%. clues that might suggest aspergillus as a pathogen in community-acquired pneumonia include a chest radiograph revealing diffuse infiltrates or new cavitation; lack of bacterial or viral cause; a preceding influenza a infection; and ... | 1998 | 9726758 |
| allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa) is an immunologically mediated lung disease which occurs predominantly in patients with asthma, and is caused by hypersensitivity to colonized aspergillus fumigatus. it is a chronic, relapsing disorder which can clinically range from mild asthma to fibrotic lung disease. the immunopathogenesis of the disease is not clearly understood. early diagnosis and aggressive therapy with oral corticosteroids can prevent the development of fibrotic lung diseas ... | 1998 | 9727282 |
| characterization and optimization of in vitro assay conditions for (1,3)beta-glucan synthase activity from aspergillus fumigatus and candida albicans for enzyme inhibition screening. | (1,3)beta-d-glucan synthase (e.c.2.4.1.34. udp-glucose: 1,3-beta-d-glucan 3-beta-glucosyl transferase) catalyzes the polymerization of glucose ([1-3]-beta-linkages) using udp-glucose as substrate. we have determined optimal in vitro conditions for the assay of (1,3)beta-glucan synthase activity from aspergillus fumigatus and candida albicans. these included lysis of cells in the following for c. albicans, 100 mm hepes, ph 8.0, 10 microm guanosine 5'-o-(3-thiotriphosphate) (gtpgammas), 2 mm ethyl ... | 1998 | 9727393 |
| chest radiographic staging in allergic bronchopulmonary aspergillosis: relationship with immunological findings. | the question of whether a chest radiographic severity staging system could be correlated with standard blood/serum diagnostic indices in allergic bronchopulmonary aspergillosis (abpa) was addressed in 41 patients. asthma and positive aspergillus fumigatus (af) serology were considered essential diagnostic inclusion criteria. eosinophil count, serum immunoglobulin (ig)e and immediate skin hypersensitivity were also tested to grade patients as "definite" or "likely" abpa. definite cases had all fi ... | 1998 | 9727800 |
| primary cutaneous aspergillosis: our experience in 10 years. | we describe nine patients with primary cutaneous aspergillosis who were diagnosed during the 10-year period between 1987 and 1996. all except one were adults. seven of those nine cases had an immunocompromised state. among the immunocompromised patients, six had burn wounds and one was a renal transplant recipient. aspergillus flavus was isolated from seven patients and a. fumigatus from one, and in one other case the species could not be determined. three patients who had total burn surface are ... | 1998 | 9733373 |
| fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide d4e1. | the fungicidal properties of the synthetic peptide d4e1 were studied with nongerminated and germinating conidia of aspergillus flavus, aspergillus fumigatus, aspergillus niger, fusarium moniliforme, and fusarium oxysporum. the minimal lethal concentrations (mlc) needed to kill 100% of germinating conidia of a. fumigatus, a. flavus, and a. niger were 12.5, 12.5, and 25 microm, respectively. the mlc value for nongerminated and germinating conidia of both fusarium spp. was 3.0 microm. except for a. ... | 1998 | 9734302 |
| molecular probes for the detection of pathogenic fungi in the presence of human tissue. | four primer systems, amplifying fragments of the gene coding for the small ribosomal subunit (18s rrna) were characterised with pure cultures of 65 medically relevant fungal species plus two mushrooms. a primer cocktail (tr1/ca1-tr2/af2) amplified 59 of 67 fungal species; the universal fungal primer 1 (uf1) in combination with the eukaryotic primers s3 or eu1 amplified 64 and 65 of 67 fungal species, respectively. the design of an additional primer (rzy1) enabled the amplification of the missing ... | 1998 | 9736163 |
| activity of voriconazole combined with neutrophils or monocytes against aspergillus fumigatus: effects of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. | voriconazole (vcz) was tested for antifungal activity against aspergillus fumigatus hyphae alone or in combination with neutrophils or monocytes. antifungal activity was measured as percent inhibition of hyphal growth in assays using the dye mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] or xtt [2, 3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2h-tetrazolium-5-carboxa nilide ]. with both assays, vcz inhibited hyphal growth at concentrations of <1 microgram/ml and was almost as activ ... | 1998 | 9736553 |
| comparison of in vitro activities of the new triazole sch56592 and the echinocandins mk-0991 (l-743,872) and ly303366 against opportunistic filamentous and dimorphic fungi and yeasts. | the in vitro antifungal activities of sch56592, mk-0991, and ly303366 against 83 isolates of acremonium strictum, aspergillus flavus, aspergillus fumigatus, aspergillus terreus, bipolaris spp., blastomyces dermatitidis, cladophialophora bantiana, fusarium oxysporum, fusarium solani, histoplasma capsulatum, phialophora spp., pseudallescheria boydii, rhizopus arrhizus, scedosporium prolificans, and sporothrix schenckii were compared. the in vitro activities of these agents against 104 isolates of ... | 1998 | 9738049 |
| insertional mutagenesis of aspergillus fumigatus. | we have investigated transformation with heterologous dna as a method for insertional mutagenesis of aspergillus fumigatus. two methods, polyethylene glycol-mediated transformation of protoplasts and electroporation of germinating spores, were used to establish conditions leading to single-copy integration of transforming dna at different genomic sites. we have assessed the effect of restriction enzyme-mediated integration (remi) for both methods. non-remi protoplast transformation led to integr ... | 1998 | 9749676 |
| [chronic necrotizing pulmonary aspergillosis resistant to antimycotic drugs and treated by partial pulmonary resection]. | a 46-year-old man with a history of left upper lobectomy for pulmonary tuberculosis was admitted to our hospital because of dilated cardiomyopathy. during hospitalization, fever and weight loss developed. the cause was suspected to be a round mass inside a cavity and a neighboring infiltrative shadow in the left upper lung field as seen on chest radiography. a percutaneous needle biopsy was done, and examination of the specimen showed an aggregate of aspergillus fumigatus hyphae. fluconazole (fc ... | 1998 | 9754002 |
| granulocyte colony-stimulating factor and azole antifungal therapy in murine aspergillosis: role of immune suppression. | outbred icr mice were immune suppressed either with hydrocortisone or with 5-fluorouracil and were infected intranasally with aspergillus fumigatus. beginning 3 days before infection some groups of mice were given recombinant human granulocyte colony-stimulating factor (g-csf), sch56592 (an antifungal triazole), or both. corticosteroid-pretreated mice responded to sch56592 and had reduced counts in lung tissue and prolonged survival. in these mice, g-csf strongly antagonized the antifungal activ ... | 1998 | 9756743 |
| structure-in vitro activity relationships of pentamidine analogues and dication-substituted bis-benzimidazoles as new antifungal agents. | twenty analogues of pentamidine, 7 primary metabolites of pentamidine, and 30 dicationic substituted bis-benzimidazoles were screened for their inhibitory and fungicidal activities against candida albicans and cryptococcus neoformans. a majority of the compounds had mics at which 80% of the strains were inhibited (mic80s) comparable to those of amphotericin b and fluconazole. unlike fluconazole, many of these compounds were found to have potent fungicidal activity. the most potent compound again ... | 1998 | 9756747 |
| in vitro antifungal activities of a series of dication-substituted carbazoles, furans, and benzimidazoles. | aromatic dicationic compounds possess antimicrobial activity against a wide range of eucaryotic pathogens, and in the present study an examination of the structures-functions of a series of compounds against fungi was performed. sixty-seven dicationic molecules were screened for their inhibitory and fungicidal activities against candida albicans and cryptococcus neoformans. the mics of a large number of compounds were comparable to those of the standard antifungal drugs amphotericin b and flucon ... | 1998 | 9756748 |
| accumulation of amphotericin b in human macrophages enhances activity against aspergillus fumigatus conidia: quantification of conidial kill at the single-cell level. | a cytofluorometric assay that allowed assessment of damage to phagocytosed aspergillus fumigatus conidia at the single-cell level was developed. after ingestion by monocyte-derived macrophages (mdms), conidia were reisolated by treatment of the cells with streptolysin o, a pore-forming toxin with lytic properties on mammalian cells but not on fungi. the counts obtained by staining of damaged conidia with propidium iodide and quantification by cytofluorometry correlated with colony counts. by the ... | 1998 | 9756757 |
| [the role of imperfect fungi in etiopathogenesis of allergic rhinitis]. | in this paper the role of imperfect fungi in etiopathogenesis of perennial rhinitis was examined. in a group of 26 patients the concentration of total ige and ige specific of candida albicans, aspergillus fumigatus, alternaria alternata, mucor racemosus and cladosporum herbarum was signified. oversensibility to imperfect fungi was confirmed in 30.8% of patients: to candida albicans in 3.8%, to aspergillus fumigatus in 11.5%, to alternaria alternata in 3.8%, to mucor racemosus in 7.6% and to clad ... | 1998 | 9760768 |
| micellar electrokinetic chromatography: a convenient alternative to colorimetric and high performance liquid chromatographic detection to monitor protease activity. | high performance capillary electrophoresis (hpce) has been exploited as an analytical method alternative to current procedures for the determination of proteolytic activity of elastases from different sources. due to some drawbacks with capillary zone electrophoresis (cze), the mode of operation employed for the assay of elastolytic activity was micellar electrokinetic chromatography (mekc). using a background electrolyte consisting of 35 mm sodium tetraborate, ph 9.3, containing 65 mm sds and 1 ... | 1998 | 9761185 |
| effects of alkaline protease or restrictocin deficient mutants of aspergillus fumigatus on human polymorphonuclear leukocytes. | several substances including proteases and restrictocin have been suggested as candidates for virulence determinants in invasive pulmonary aspergillosis. however, the roles of such substances are not well understood. this study compared the in vitro suppressive effects of aspergillus fumigatus culture filtrates (acfs), on the functions of human polymorphonuclear leukocytes (pmnls), the principal cells in the host defence against aspergillus hyphae, from a clinically isolated wild-type and isogen ... | 1998 | 9762788 |
| probing the active site of mitogillin, a fungal ribotoxin. | fungal ribotoxins, such as mitogillin and the related aspergillus toxins restrictocin and alpha-sarcin, are highly specific ribonucleases, which inactivate the ribosome enzymatically by cleaving the eukaryotic 28s rna of the large ribosomal subunit at a single phosphodiester bond. the site of cleavage occurs between g4325 and a4326, which are present in a 14-base sequence (the alpha-sarcin loop) conserved among the large subunit rrnas of all living species. the amino acid residues involved in th ... | 1998 | 9767570 |
| [evaluation of an elisa for the detection of anti-aspergillus fumigatus antibodies]. | an indirect microelisa system for the detection of anti-aspergillus fumigatus antibodies using human anti-igg conjugated to peroxidase was standardized. serum samples of two patients having an aspergilloma diagnosed by clinical, microbiological, and serological criteria were used as positive controls. in addition, 119 serum samples of blood donors and 216 serum samples of patients having chronic pneumopathies were also studied. the standardized system may be used as a diagnostic complement for i ... | 1992 | 9768220 |
| aspergillus nidulans infection in chronic granulomatous disease. | chronic granulomatous disease (cgd) is a rare inherited disorder of the nadph oxidase complex in which phagocytes are defective in generating reactive oxidants. as a result, patients with cgd suffer from recurrent bacterial and fungal infections. the most common fungal infections are caused by aspergillus species. aspergillus nidulans is a rare pathogen in most patient populations with quantitative or qualitative neutrophil defects. we have reviewed all cases in which a. nidulans was isolated fr ... | 1998 | 9772923 |
| comparison of pcr, (1-->3)-beta-d-glucan and galactomannan assays in sera of rats with experimental invasive aspergillosis. | we compared pcr, galactomannan detection assay using a latex agglutination test and (1-->3)-beta-d-glucan detection assay in detecting infection in rats experimentally infected with aspergillus fumigatus. on day 2 after inoculation, (1-->3)-beta-d-glucan and nested pcr were positive for 80%, while galactomannan detection assay was positive for 60%. in addition, the positive result of nested pcr (87.5%) was higher than those of galactomannan detection assay (75%) and (1-->3)-beta-d-glucan (71.4%) ... | 1998 | 9773954 |
| [intrahospital necrotizing pneumonia]. | 1998 | 9774886 | |
| does hydrocortisone modify the in vitro susceptibility of aspergillus fumigatus to itraconazole and amphotericin b? | to analyse if hydrocortisone could modify the in vitro susceptibility of aspergillus fumigatus to antifungal drugs, we developed a procedure to test the susceptibility of an a. fumigatus strain to amphotericin b and itraconazole, grown in the presence and in the absence of hydrocortisone. conidia were germinated in the presence or the absence of hydrocortisone in czapek medium without antifungal drug. a dilution of these cultures (5x10(3) conidia ml(-1)) was spread onto czapek-agarose plates con ... | 1998 | 9776816 |
| polar lipids of aspergillus fumigatus, a. niger, a. nidulans, a. flavus and a. terreus. | little is known of the phospholipid composition of aspergillus species. the aim of this study was to determine individual phospholipid analogues in aspergillus. twenty-nine clinical and environmental isolates from five aspergillus species were analysed. fast atom bombardment mass spectrometric data were considered in two ranges, m/z 190-500 and m/z 500-1000, to facilitate the recognition of major fatty acyl groups and phospholipids. quantitative comparison of major anions in both m/z ranges was ... | 1998 | 9776825 |
| the identification and phylogenetic relationship of pathogenic species of aspergillus based on the mitochondrial cytochrome b gene. | to study the identification and phylogeny of pathogenic isolates of aspergillus, we designed primers from known cytochrome b amino acid sequences. using these primers, 426 bp fragments of a mitochondrial (mt) cytochrome b gene were amplified by polymerase chain reaction (pcr), directly sequenced, and compared among aspergillus fumigatus, a. flavus, a. niger, a. terreus and emericella nidulans. except for e. nidulans, all strains produced the 426 bp fragment by pcr. the e. nidulans strains demons ... | 1998 | 9776828 |
| concentrations of airborne aspergillus compared to the incidence of invasive aspergillosis: lack of correlation. | air sampling of the rooms and corridors of the oncology wards of the hospital was carried out over a 54-week period to assess the concentration of viable aspergillus conidia. a. fumigatus and a. flavus were recovered at a mean of 1.83 cfu m-3 air sampled. individual samplings yielded concentrations of up to 11.6 cfu m-3. other aspergillus spp. were recovered at a mean of 2.38 cfu m-3 (maximum 32.6 cfu m-3). concentration was not correlated with season or hospital ward. review of autopsy results ... | 1998 | 9776829 |
| specific identification of penicillium marneffei by a polymerase chain reaction/hybridization technique. | penicillium marneffei has been described recently as a cause of an emerging mycotic infection in hiv-infected patients. a pcr/hybridization assay was developed to rapidly identify this pathogen. the nucleotide sequence of the 631-bp region of 18s ribosomal dna of p. marneffei was determined using the standard dideoxy chain termination method. an oligonucleotide probe was designed on the basis of the analysed sequences of p. marneffei and 18s rdna sequences of other fungi in the genbank database. ... | 1998 | 9776830 |
| kinetic study of host defense and inflammatory response to aspergillus fumigatus in steroid-induced immunosuppressed mice. | the sequential pathogenesis of pulmonary aspergillosis was studied and the role of inflammatory cytokines in host response to aspergillus fumigatus was characterized in immunocompetent and immunosuppressed mice. two distinct phases were observed in immunocompetent mice: first, an intense clearance of a. fumigatus occurred, possibly through alveolar macrophages and recruited neutrophils (pmnl), accompanied by rapid release of tumor necrosis factor-alpha, interleukin (il)-6, and il-1beta, and seco ... | 1998 | 9780270 |
| mechanism and specificity of immunoprotection induced by mycobacterial proteins against experimental tuberculosis in mice. | mechanism of immunoprotection and specificity of two highly immunoprotective mycobacterial proteins, viz. 71 and 30 kda were investigated. the adoptive transfer studies indicated that immunoprotection was mainly mediated by cooperative effect of cd4+ and cd8+ (66.7-73.3% on the basis of percent survival) which was further enhanced marginally by supplementation of b cells, natural killer cells, dendritic cells, macrophages and other immune cells. the specificity studies indicated that both the pr ... | 1998 | 9782782 |
| immunological characterization of asp f 2, a major allergen from aspergillus fumigatus associated with allergic bronchopulmonary aspergillosis. | the 37-kda recombinant protein asp f 2, encoding an allergen of aspergillus fumigatus, was expressed in a prokaryotic expression system and immunologically evaluated for its functional and structural properties. the open reading frame for a 310-amino-acid-long protein was shown to encode a signal peptide of 31 amino acids. a native 37-kda culture filtrate protein and a 55-kda mycelial glycoprotein (gp55) exhibited complete n-terminal sequence homology to asp f 2. a genbank search for homologous ... | 1998 | 9784519 |
| in-vitro activities of amphotericin b, itraconazole and voriconazole against 150 clinical and environmental aspergillus fumigatus isolates. | the in-vitro activity of amphotericin b, itraconazole and voriconazole against 130 clinical and 20 environmental aspergillus fumigatus isolates was tested with an agar dilution method using rpmi 1640 medium. itraconazole-susceptible (af71) and -resistant (af90) isolates were included in each test and all isolates were tested in duplicate. geometric mean mic values (ranges) at 48 h and 72 h were, respectively, amphotericin b, 0.91 (0.25-2) and 1.50 (1-4) mg/l; itraconazole, 0.25 (0.06-1) and 0.48 ... | 1998 | 9786480 |
| molecular concepts of ige-initiated inflammation in atopic and nonatopic asthma. | atopic and nonatopic (intrinsic) asthmatics were characterized by a broadly conserved bronchial mucosal proeosinophilic cytokine network in which il-5 appears to play a key role. inappropriate ige-mediated mechanisms may occur in asthma, irrespective of its atopic status, as suggested by elevated serum ige concentrations and bronchial mucosal expression of fcepsilonri, il-4, il-13, iepsilon, and cepsilon. in general, these observations support the concept that these subtypes of asthma, despite s ... | 1998 | 9788702 |
| renal abscesses in an aids patient caused by aspergillus fumigatus. | aspergillus abscesses of the kidneys are very rare complications of aids which usually have fatal outcome. the 4 patients described in the literature, 2 of whom had bilateral involvement, died due to this opportunistic infection. | 1998 | 9792986 |
| [specific fungal antisera production in rabbits]. | a method for fungic antisera production against aspergillus fumigatus, histoplasma capsulatum, paracoccidioides brasiliensis and coccidioides immitis in rabbits was evaluated. intradermic via and antigen in the dilution used for routine tests (ud) were employed to produce positive control serum for immunodiffusion test in agar gel. a. fumigatus, h. capsulatum and c. immitis antigens were prepared as described in cdc's procedure manual, p. brasiliensis antigen was prepared as previously described ... | 1998 | 9793140 |
| cerebral aspergillosis: long term efficacy and safety of liposomal amphotericin b in kidney transplant. | 1998 | 9794583 | |
| fatal hemorrhage from rupture of the intracranial internal carotid artery caused by aspergillus arteritis. | to the best of our knowledge, this is the first reported case of combined intracranial and extracranial hemorrhage due to aspergillus cerebral arteritis. knowledge of the imaging and the importance of early diagnosis and treatment are emphasized. a 78 year old man developed progressive right-sided visual impairment and diplopia. magnetic resonance imaging demonstrated a mass lesion located in the right orbital apex, with extension to the cavernous sinus and the right middle cranial fossa. cerebr ... | 1998 | 9795962 |
| effects of aspergillus fumigatus phytase on phosphorus digestibility, phosphorus excretion, bone strength and performance in pigs. | phytic-phosphorus has a very low bioavailability for monogastric animals and the non-utilized mineral contributes to the phosphorus (p) pollution problems. phytases may ameliorate phytic-p antinutritive properties. however, phytases are very sensitive to the pelleting temperature commonly used for compound feed production and thus the challenge to produce a more thermostable phytase is very important. pure aspergillus fumigatus phytase (afp) has the ability to refold into a native-like fully act ... | 1998 | 9795986 |
| sordarins: in vitro activities of new antifungal derivatives against pathogenic yeasts, pneumocystis carinii, and filamentous fungi. | gm 193663, gm 211676, gm 222712, and gm 237354 are new semisynthetic derivatives of the sordarin class. the in vitro antifungal activities of gm 193663, gm 211676, gm 222712, and gm 237354 against 111 clinical yeast isolates of candida albicans, candida kefyr, candida glabrata, candida parapsilosis, candida krusei, and cryptococcus neoformans were compared. the in vitro activities of some of these compounds against pneumocystis carinii, 20 isolates each of aspergillus fumigatus and aspergillus f ... | 1998 | 9797217 |
| antifungal efficacy, safety, and single-dose pharmacokinetics of ly303366, a novel echinocandin b, in experimental pulmonary aspergillosis in persistently neutropenic rabbits. | ly303366 is a novel semisynthetic derivative of echinocandin b and a potent inhibitor of fungal (1,3)-beta-d-glucan synthase. the antifungal efficacy and safety of ly303366 were investigated in treatment and prophylaxis of primary pulmonary aspergillosis due to aspergillus fumigatus in persistently neutropenic rabbits. treatment study groups were either not treated (controls) or treated with amphotericin b (amb) at 1 mg/kg of body weight per day or with ly303366 at 1, 5, 10, and 20 mg/kg/day. in ... | 1998 | 9797223 |
| organism-dependent fungicidal activities of azoles. | we investigated the antifungal activities of itraconazole and voriconazole on aspergillus species by time kill studies, and the results were compared with those obtained for candida species. exposure of aspergillus fumigatus conidia to varying concentrations (1.25 to 10 microg/ml) of itraconazole and voriconazole resulted in cellular death; the cytocidal effect was time and concentration dependent. in contrast, no killing of candida albicans occurred in the presence of itraconazole and voriconaz ... | 1998 | 9797246 |
| comparison of the thermostability properties of three acid phosphatases from molds: aspergillus fumigatus phytase, a. niger phytase, and a. niger ph 2.5 acid phosphatase. | enzymes that are used as animal feed supplements should be able to withstand temperatures of 60 to 90 degrees c, which may be reached during the feed pelleting process. the thermostability properties of three histidine acid phosphatases, aspergillus fumigatus phytase, aspergillus niger phytase, and a. niger optimum ph 2.5 acid phosphatase, were investigated by measuring circular dichroism, fluorescence, and enzymatic activity. the phytases of a. fumigatus and a. niger were both denatured at temp ... | 1998 | 9797305 |
| [tracheobronchial aspergillosis in a patient with buckley's syndrome]. | 1998 | 9798465 | |
| [usefulness of bronchoalveolar lavage in the renal transplant patient with suspected respiratory infection]. | in this retrospective study we aimed to assess the diagnostic yield of bronchoalveolar lavage (bal) in kidney transplant patients who were suspected of having severe respiratory infection or in whom empirical antibiotic treatment had failed. all bal procedures performed on kidney transplanted patients suspected of having respiratory infections between january 1, 1988 and july 31, 1996 were analyzed. bal was carried out in the standard way and samples were sent for cytologic and bacteriologic stu ... | 1998 | 9803276 |
| disseminated aspergillus fumigatus infection: case report. | a case of disseminated aspergillus fumigatus infection is reported in a 43 year old ugandan female with no known immune system disorder(not neutropenic and hiv-1 sero negative). she presented with multiple cutaneous ulcers, recurrent empyema thoracis, a past history of intra-abdominal abscess and bowel infarction. empirical treatment for tuberculosis was previously given without improvement. a diagnosis of aspergillus fumigatus based on a combination of tissue wet potassium hydroxide preparation ... | 1998 | 9803639 |
| interleukin-9 promotes allergen-induced eosinophilic inflammation and airway hyperresponsiveness in transgenic mice. | human atopic asthma is a complex heritable inflammatory disorder of the airways associated with clinical signs of allergic inflammation and airway hyperresponsiveness. recent studies demonstrate that the degree of airway responsiveness is strongly associated with interleukin (il)-9 expression in murine lung. to investigate the contribution of il-9 to airway hyperresponsiveness, and to explore directly its relationship to airway inflammation, we studied transgenic mice overexpressing il-9. in thi ... | 1998 | 9806735 |
| oxidized low density lipoprotein inhibits the hemolytic activity of asp-hemolysin from aspergillus fumigatus. | we have examined the effect of chemically modified human low density lipoproteins (ldls), acetylated ldl and oxidized ldl, on the hemolytic activity of asp-hemolysin. oxidized ldl, but not acetylated ldl, inhibited the hemolytic activity of this toxin. the inhibitory effects of oxidized ldl increased with the time of cu(2+)-induced ldl oxidation. similar inhibition was observed in the filtrate which was separated from the incubation mixture of asp-hemolysin with oxidized ldl (for 2 h of oxidatio ... | 1998 | 9809428 |
| imipenem inhibits in vitro activity of amphotericin b directed against aspergillus fumigatus. | a decrease of the susceptibility of aspergillus fumigatus strains to amphotericin b was found when tested in combination with high concentrations (128-2048 mg/l) of the antibacterial agent imipenem by checkerboard titration and agar diffusion assay. only bacteriologically active imipenem showed the antagonism. the mechanism of action is unknown. however, imipenem and amphotericin b did not react directly, as shown by checkerboard titration with bacterial strains as well as by hplc analysis. it i ... | 1995 | 9810647 |
| aspergillus and lung transplant recipients: a mycologic and molecular epidemiologic study. | after lung transplantation, filamentous fungi and more particularly aspergillus fumigatus are commonly isolated, although the origin of contamination is unclear. | 1998 | 9811404 |
| cytokine- and t helper-dependent lung mucosal immunity in mice with invasive pulmonary aspergillosis. | the role of cytokine- and t helper (th)-dependent lung mucosal antifungal immunity in murine invasive pulmonary aspergillosis (ipa) was investigated. intact or leukopenic dba/2 mice were resistant or highly susceptible, respectively, to infection caused by multiple intranasal injections of viable aspergillus fumigatus conidia. resistance was associated with unimpaired innate antifungal activity of pulmonary phagocytic cells, concomitant with high-level production of tumor necrosis factor (tnf)-a ... | 1998 | 9815229 |
| aspergillus fumigatus strains recovered from immunocompromised patients (icp): subtyping of strains by rapd analysis. | invasive pulmonary aspergillosis in immunocompromised patients (icp) is the second most frequent opportunistic fungal infection. the causative organism includes 16 species of aspergillus, of which a. fumigatus dominates the ubiquitous incidence of invasive or allergic broncho-pulmonary aspergillosis (abpa). the definitive diagnosis of invasive aspergillosis is difficult. we have analyzed 24 strains of a. fumigatus recovered from icp using the rapd technique. the profiles generated with the 20 pr ... | 1998 | 9818093 |
| in-vitro testing of susceptibility to amphotericin b is a reliable predictor of clinical outcome in invasive aspergillosis. | invasive aspergillosis is a life-threatening fungal infection which, in neutropenic patients, is associated with an extremely high mortality rate despite optimal treatment. in order to investigate microbiological risk factors for treatment failures in more detail, aspergillus spp. obtained from 29 patients with haematological diseases after myelo-ablative chemotherapy and bone marrow transplantation were analysed for their susceptibility to amphotericin b in vitro and this was compared with clin ... | 1998 | 9818749 |
| comparison of the in-vitro activity of voriconazole (uk-109,496), itraconazole and amphotericin b against clinical isolates of aspergillus fumigatus. | voriconazole was compared with amphotericin b and itraconazole by a modification of the nccls microdilution reference method for yeasts against 62 clinical isolates of aspergillus fumigatus. mics of voriconazole were slightly lower than those of amphotericin b and itraconazole. the mic of voriconazole at which 90% of isolates were inhibited was 1 mg/l and the mic range was 0.25-2 mg/l. voriconazole is a new antifungal agent with potential for use in the treatment of a. fumigatus infections. | 1998 | 9818755 |
| sch56592 treatment of murine invasive aspergillosis. | mice were immunosuppressed using corticosteroids and infected with conidia of aspergillus fumigatus. beginning 1 day after infection, mice were treated orally either with noble agar or with sch56592 suspended in noble agar, or intraperitoneally with amphotericin b. sch56592 prolonged survival and reduced lung tissue counts of a. fumigatus, while amphotericin b had marginal benefit. sch56592 merits further development for treatment of aspergillosis. | 1998 | 9818757 |
| hemoptysis and a lung mass in a 51-year-old patient with asthma. | 1998 | 9824030 | |
| tumor necrosis factor alpha enhances antifungal activities of polymorphonuclear and mononuclear phagocytes against aspergillus fumigatus. | invasive aspergillosis is a serious complication in immunocompromised patients. the effects of recombinant human tumor necrosis factor alpha (tnf-alpha) on antifungal activities of human neutrophils (polymorphonuclear leukocytes [pmns]), human monocytes (mncs), and rabbit pulmonary alveolar macrophages (pams) against aspergillus fumigatus were studied. the percentage of pmn-induced hyphal damage was increased after 30 min of incubation of pmns with 0.1 ng of tnf-alpha per ml at 37 degreesc (p = ... | 1998 | 9826384 |
| detection of aspergillus fumigatus pcr products by a microtitre plate based dna hybridisation assay. | to develop a dna based plate hybridisation assay for the detection of polymerase chain reaction (pcr) products amplified from aspergillus fumigatus dna; and to determine the sensitivity of this technique and compare it with southern blotting. | 1998 | 9828823 |
| disseminated aspergillosis in a premature neonate. | 1998 | 9830596 | |
| fungal colonisation and fluconazole therapy in acute liver disease. | fungal infection, particularly with candida spp., has been identified as an important cause of morbidity and mortality in patients with acute liver failure. fungal colonisation of superficial mucosal sites usually precedes invasive infection. we investigated colonisation patterns in patients with acute liver disease receiving fluconazole therapy in order to investigate the possibility of emergence of fluconazole-resistant c. albicans or other species. | 1998 | 9831360 |
| identification of a polyketide synthase gene (pksp) of aspergillus fumigatus involved in conidial pigment biosynthesis and virulence. | aspergillus fumigatus is an important pathogen of the immunocompromised host causing pneumonia and invasive disseminated disease with high mortality. previously, we identified a mutant strain (white, w) lacking conidial pigmentation and, in addition, the conidia showed a smooth surface morphology, whereas wild-type (wt) conidia are grey-green and have a typical ornamentation. w conidia appeared to be less protected against killing by the host defence, e.g., were more susceptible to oxidants in v ... | 1998 | 9832321 |
| a novel method of purifying lung surfactant proteins a and d from the lung lavage of alveolar proteinosis patients and from pooled amniotic fluid. | a simple procedure has been developed for the purification of the surfactant proteins sp-a and sp-d from lung lavage of patients with alveolar proteinosis. the sp-d is purified by fractionation of the supernatant obtained after spinning the lavage at 10000 x g for 40 min, while the bulk of the sp-a is purified by fractionation of the pellet. the supernatant is applied to a maltosyl-agarose column and the bound sp-d is specifically eluted using mncl2. the pellet is solubilised in 6 m urea and, fo ... | 1998 | 9839935 |
| a 1,3-beta-glucanosyltransferase isolated from the cell wall of aspergillus fumigatus is a homologue of the yeast bgl2p. | a 1,3-beta-glucanosyltransferase which introduces intrachain 1,6-beta linkages into 1,3-beta-glucan was isolated from the cell wall of aspergillus fumigatus. the biochemical and molecular characterization of the a. fumigatus transferase showed it was homologous to the saccharomyces cerevisiae and candida albicans transferase bgl2p. a null mutant constructed in a. fumigatus by gene replacement did not show a distinct phenotype from the parental strain. the putative function of this major cell-wal ... | 1998 | 9846753 |
| in-vitro and in-vivo susceptibility of aspergillus fumigatus to a novel conjugated styryl ketone. | we investigated the in-vitro and in-vivo susceptibility of aspergillus fumigatus to the novel conjugated styryl ketone nc1175 and the results were compared with those obtained for amphotericin b and itraconazole. all 20 clinical isolates of a. fumigatus examined were susceptible to nc1175 (mic = 5.54 +/- 2.48 mg/l; range 2.92-11.68 mg/l), and the minimum lethal concentration (mlc) was only twice the mic, suggesting that nc1175 is fungicidal. the mean mic values of amphotericin b (1.22 +/- 0.58 m ... | 1998 | 9848441 |
| toxicity and therapeutic efficacy of amphotericin b delivered through cholesterol hemisuccinate vesicles in the treatment of experimental murine aspergillosis. | we have studied the toxicity and therapeutic efficacy of amphotericin b in cholesterol hemisuccinate vesicles (abcv) in the treatment of invasive aspergillosis in balb/c mice. the toxicity of amphotericin b was significantly reduced when delivered through cholesterol hemisuccinate vesicles as compared to deoxycholate suspension (amb(doc)) as evidenced by reduced nephrotoxicity in balb/c mice, lower in-vitro toxicity to erythrocytes and higher maximum tolerated dose. the latter increased from 2 m ... | 1998 | 9848448 |
| induction of differentiation and apoptosis in human promyelocytic leukemia hl60 cell line by a new type of steroids. | mer-nf8054x is a new type of steroid whose structure has been established as 11-oxo-18, 22-cycloergosta-6, 8(14)-diene-3beta, 5beta, 9beta, 23s-tetraol (an 18, 22-cycloergostane), which has been reported to have antifungal activity against aspergillus fumigatus. however, other biological activities are unknown. herein, we reported that mer-nf8054x inhibited cell growth of hl60 human leukemia cells, when used either singly or in combination with retinoic acid (ra). in addition, mer-nf8054x alone ... | 1998 | 9851872 |
| [aspergillus fumigatus muscle abscess revealing invasive aspergillosis in a patient with aids]. | 1998 | 9856131 | |
| [invasive pulmonary aspergillosis in the course of allergic pulmonary alveolitis]. | 20 years old man with history of acute allergic alveolitis 7 years ago, was admitted to hospital because of dyspnea, fever and cough after massive exposition to organic dust. corticosteroids were introduced. during 3-weeks treatment respiratory failure progressed. chest x-ray showed massive bilateral nodular opacities. antibiotic therapy was not effective. the autopsy revealed invasive pulmonary aspergillosis. presence of precipitins to aspergillus fumigatus was confirmed postmortem. diagnostic ... | 1998 | 9857668 |
| in vitro evaluation of voriconazole against clinical isolates of yeasts, moulds and dermatophytes in comparison with itraconazole, ketoconazole, amphotericin b and griseofulvin. | the in vitro activity of voriconazole (uk-109, 496), a new antifungal triazole derivative, against 650 clinical isolates of yeasts, moulds and dermatophytes was compared with that of itraconazole, ketoconazole, amphotericin b and griseofulvin. the geometric means of the minimum inhibitory concentrations (mics) of voriconazole were 0.05 microgram ml-1 against yeasts (n = 187), 0.58 microgram ml-1 against moulds (n = 260) and 0.08 microgram ml-1 against dermatophytes (n = 203). the overall activit ... | 1998 | 9861837 |
| immunodominant peptide epitopes of allergen, asp f 1 from the fungus aspergillus fumigatus. | aspergillus fumigatus ribotoxin asp f 1 is a major allergen with ige binding activity to serum of a majority of patients with allergic bronchopulmonary aspergillosis (abpa). the ige binding epitopes or the t-cell stimulatory peptides of this molecule have not been studied. in the present investigation, we have synthesized linear decapeptides spanning the whole molecule of asp f 1 and analyzed their ige binding properties. we have also synthesized peptides based on their possible t-cell stimulato ... | 1998 | 9864052 |
| [allergic bronchopulmonary aspergillosis effectively treated with itraconazole]. | a 23-year-old man with bronchial asthma presented with fever, cough, and sputum. a chest x-ray examination showed pulmonary infiltrations in the left upper and lower lung fields with central bronchiectasis. although his temperature came down with antibiotics, pulmonary infiltrations persisted with cough and sputum. following bronchoscopy and an allergological examination, the patient was given a diagnosis of allergic bronchopulmonary aspergillosis (abpa) based on rosenberg's criteria, including ... | 1998 | 9866981 |
| production of amylase by aspergillus fumigatus utilizing alpha-methyl-d-glycoside, a synthetic analogue of maltose, as substrate. | a strain of aspergillus fumigatus isolated from soil was able to produce biomass and high amylase activities in media containing alpha-methyl-d-glucoside (alphamg), a synthetic analogue of maltose, as the only carbon source. alphamg was a more effective inducer than starch and maltose at the same concentration: alphamg cultures produced about 3 times more alpha-amylase and glucoamylase activity than starch cultures. maximum production of alpha-amylase (60 u/mg) and glucoamylase (130 u/mg) was ob ... | 1998 | 9867468 |
| modeling, synthesis and biological activity of novel antifungal agents (1). | homology modeling of candida lanosterol c-14 demethylase, synthesis and in vitro antifungal activities of cyclohexyl analogs of restricticin are described. | 1998 | 9873440 |
| synthesis of novel antifungal agents (2). | synthesis of novel cyclohexyl analogs of restricticin using intramolecular radical cyclization and their in vitro and in vivo antifungal activities are described. | 1998 | 9873441 |
| early pulmonary lesions in turkeys produced by nonviable aspergillus fumigatus and/or pasteurella multocida lipopolysaccharide. | this study assessed the potential of lipopolysaccharide (lps) purified from pasteurella multocida to cause pulmonary pathology or exacerbate lesions produced by gamma-irradiated nonviable aspergillus fumigatus conidia when administered via the intra-air sac route in turkeys. lps provoked suppurative airsacculitis, pleuritis, and pneumonia. nonviable conidia produced airsacculitis and transient pneumonitis but did not elicit multinucleate giant cells, which are a feature of the inflammatory proce ... | 1998 | 9876847 |
| susceptibility of convalescent turkeys to pulmonary aspergillosis. | pulmonary lesions resulting from aspergillus fumigatus inoculation were assessed in convalescent turkeys and compared with those in previously noninoculated (control) turkeys. in addition, lesions observed in small beltsville white (sbw) turkeys were compared with those in broad-breasted white (bbw) turkeys challenged with the same inoculum. turkeys were challenged by unilateral posterior thoracic air sac (ptas) inoculation, rechallenged via the contralateral air sac after 5 wk, and then necrops ... | 1998 | 9876849 |
| studies on pulmonary and systemic aspergillus fumigatus-specific ige and igg antibodies in horses affected with chronic obstructive pulmonary disease (copd). | inhalant exposure to aspergillus fumigatus (asp. f.) antigens induces marked inflammatory and immunological alterations in the lungs of horses affected with chronic obstructive pulmonary disease (copd). in this study we investigated the role of specific allergen(s) present in asp. f. on systemic and pulmonary ige and igg responses in control and copd-affected horses, using an enzyme-linked immunosorbent assay (elisa) and immunoblotting techniques. compared with controls, horses affected with cop ... | 1998 | 9880102 |
| the evolutionary biology and population genetics underlying fungal strain typing. | strain typing of medically important fungi and fungal population genetics have been stimulated by new methods of tapping dna variation. the aim of this contribution is to show how awareness of fungal population genetics can increase the utility of strain typing to better serve the interests of medical mycology. knowing two basic features of fungal population biology, the mode of reproduction and genetic differentiation or isolation, can give medical mycologists information about the intraspecifi ... | 1999 | 9880478 |
| suppression of coronary vasculitis in a murine model of kawasaki disease using an angiogenesis inhibitor. | coronary arteritis can be induced in c57bl/6 mice with a single intraperitoneal (ip) injection of lactobacillus casei cell fragments. histologic sections resemble the vasculitis and aneurysms observed in the medium-sized coronary arteries of children with kawasaki disease. since endothelial cells could play an important role in the development of vasculitis, a recently described angiogenesis inhibitor that is not an immunosuppressive agent, agm-1470 (derived from aspergillus fumigatus), was used ... | 1999 | 9884364 |
| [pulmonary aspergilloma clearly demonstrated by digital subtraction bronchography]. | a 69-year-old woman was admitted because of persistent productive cough, low-grade fever and abnormal pulmonary infiltrates. chest roentgenograms revealed right pulmonary cavitary lesions and infiltrates in both upper pulmonary fields. digital subtraction bronchography (dsbg) was performed to detect for the presence of pulmonary cavitary lesions. double-contrast dsbg images clearly revealed marked bronchiectasis in the right pulmonary cavity with aspergilloma in the cavitary lesions. aspergillom ... | 1998 | 9893429 |
| cutaneous purulent aspergillosis in a young man with chronic granulomatous disease. | we describe a case of cutaneous purulent aspergillosis in a 19-year-old man with chronic granulomatous disease (cgd) treated successfully with a 6-month regimen of itraconazole. the therapeutic effect of the drug was seen after 1 month of administration. surgical treatment of the skin lesions, although first planned, was not necessary. | 1998 | 9916460 |
| antisporulating and respiration-inhibitory effects of essential oils on filamentous fungi. | sporulation of four species of filamentous fungi, namely aspergillus fumigatus, fusarium solani, penicillium expansum and rhizopus oryzae, was suppressed by gaseous contact with citron, lavender and thyme oils and, to a lesser extent, by that of perilla and tea tree oils. lemongrass and cinnamon bark oils were scarcely active. the antisporulating effect of the essential oils was not observed when they were applied as a solution, indicating that their vapours were the active form. moreover, expos ... | 1998 | 9916464 |
| qualitative identification of volatile metabolites from two fungi and three bacteria species cultivated on two media. | two fungal species, aspergillus fumigatus and penicillium brevicompactum and three bacteria, klebsiella pneumoniae, enterobacter agglomerans and streptomyces albus were cultivated on two media, malt extract agar and dichloran glycerol agar. the volatile metabolite samples from the cultures were adsorbed on tenax ta and analyzed qualitatively by thermal desorption gas chromatography and with a mass selective detector. various hydrocarbons, alcohols, ketones, esters and terpenes were identified. t ... | 1998 | 9919382 |
| isolation of csm1 encoding a class v chitin synthase with a myosin motor-like domain from the rice blast fungus, pyricularia oryzae. | a gene encoding a chitin synthase with a myosin motor-like domain (csm1) was isolated from pyricularia oryzae using a pcr fragment amplified from a fungal chitin synthase conserved region. the deduced amino acid sequence of csm1 is homologous to that of csma of aspergillus nidulans (65% identity). the putative gene product of csm1 is consisted of the myosin motor-like domain and a chitin synthase domain as in a. nidulans csma. the chitin synthase domain of its c-terminus was also homologous to a ... | 1999 | 9919661 |
| [pulmonary cavitation lesions in patients infected with the human immunodeficiency virus: an analysis of a series of 78 cases]. | to assess the clinical, radiologic and microbiological features of lung cavitation and hiv infection. evaluation of the differences related to this disease in the last years. | 1998 | 9922954 |
| comparison of virulence between clinical and environmental isolates of aspergillus fumigatus. | using a mixed infection model of murine invasive pulmonary aspergillosis, the comparative virulence of three clinical and four environmental isolates of aspergillus fumigatus was investigated after intranasal inoculation. coloured conidiospore mutants were first derived from clinical strains by ultraviolet mutagenesis and then compared with the parental strains and environmental strains. when the slight reductions in virulence associated with the spore colour mutations were taken into account, s ... | 1998 | 9923518 |
| biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): molecular size, glycosylation pattern, and engineering of proteolytic resistance. | phytases (myo-inositol hexakisphosphate phosphohydrolases) are found naturally in plants and microorganisms, particularly fungi. interest in these enzymes has been stimulated by the fact that phytase supplements increase the availability of phosphorus in pig and poultry feed and thereby reduce environmental pollution due to excess phosphate excretion in areas where there is intensive livestock production. the wild-type phytases from six different fungi, aspergillus niger, aspergillus terreus, as ... | 1999 | 9925554 |
| biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties. | supplementation with phytase is an effective way to increase the availability of phosphorus in seed-based animal feed. the biochemical characteristics of an ideal phytase for this application are still largely unknown. to extend the biochemical characterization of wild-type phytases, the catalytic properties of a series of fungal phytases, as well as escherichia coli phytase, were determined. the specific activities of the fungal phytases at 37 degreesc ranged from 23 to 196 u. (mg of protein)-1 ... | 1999 | 9925555 |
| pulmonary pearls. allergic bronchopulmonary aspergillosis. | 1998 | 9926702 | |
| sino-orbital aspergillosis in acquired immunodeficiency syndrome. | to describe the clinical features, causes, imaging characteristics, treatment, and outcome of patients with the acquired immunodeficiency syndrome (aids) and sino-orbital aspergillosis. | 1999 | 9930161 |
| role of tnf-alpha in pulmonary host defense in murine invasive aspergillosis. | invasive pulmonary aspergillosis is a common and devastating complication of immunosuppression, whose incidence has increased dramatically in tandem with the increase in the number of immunocompromised patients. given the role of tnf-alpha in other pulmonary infections, we hypothesized that tnf-alpha is an important proximal signal in murine invasive pulmonary aspergillosis. intratracheal challenge with aspergillus fumigatus conidia in both neutropenic (cyclophosphamide-treated) and nonneutropen ... | 1999 | 9973423 |
| comparative study of susceptibilities of germinated and ungerminated conidia of aspergillus fumigatus to various antifungal agents. | conidia are used as inocula for the in vitro susceptibility testing of aspergillus fumigatus. since the mic is defined on the basis of visible mycelial growth, conidia should germinate and produce sporelings (germinated conidia) for monitoring of the growth inhibition and fungicidal activity of a drug. if a compound is capable of inhibiting germination of conidia while affecting or not affecting the growth of the organism, the mic obtained will be the concentration of the drug required for the i ... | 1999 | 9986876 |
| fate of transforming dna in pathogenic fungi. | genetic engineering is an important tool in helping us to define the molecular basis of pathogenicity and is also useful in helping us to identify new therapeutic targets in pathogenic fungi. molecular genetic manipulation of micro-organisms requires the development of plasmid-mediated transformation systems that include: (i) infusion of exogenous dna into recipient cells, (ii) expression of genes present on the incoming dna, and (iii) stable maintenance and replication of the inserted dna leadi ... | 1998 | 9988490 |
| antifungal drug resistance in pathogenic fungi. | failures of drug treatment in fungal infections combined with improvements in performances and standardization of antifungal susceptibility testing have drawn attention to the problem of antifungal resistance and its underlying mechanisms. resistance of candida species and cryptococcus neoformans to flucytosine (5fc) develops during monotherapy. acquired resistance results from a failure to metabolize 5fc to 5futp and 5fdump, or from the loss of feedback control of pyrimidine biosynthesis. a com ... | 1998 | 9988500 |
| mycological control and surveillance of biological waste and compost. | this paper presents some recent developments regarding current work on hygienic aspects, in particular the presence and dispersion of fungi (e.g. aspergillus fumigatus), of biological waste and compost. | 1998 | 9988502 |