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in vitro susceptibility testing of dermatophytes isolated in goiania, brazil, against five antifungal agents by broth microdilution method.the antifungal activities of fluconazole, itraconazole, ketoconazole, terbinafine and griseofulvin were tested by broth microdilution technique, against 60 dermatophytes isolated from nail or skin specimens from goiania city patients, brazil. in this study, the microtiter plates were incubated at 28 masculinec allowing a reading of the minimal inhibitory concentration (mic) after four days of incubation for trichophyton mentagrophytes and five days for t. rubrum and microsporum canis. most of th ...200919229384
relation between lipophilicity of alkyl gallates and antifungal activity against yeasts and filamentous fungi.the antifungal activity of a complete series of 15 n-alkyl gallates and six analogues acting against a representative panel of opportunistic pathogenic fungi was studied in order to analyze their role in: the importance of the fungi tested, the importance of the hydroxyls, the influence of the chain length and the hydrophobicity of the compounds. it was demonstrated that dermatophytes were the most susceptible species and that hydroxyls appear to be necessary but not sufficient for the activity. ...200919232493
competence of the housefly, musca domestica, as a vector of microsporum canis under experimental conditions.the role of musca domestica linnaeus as a vector of the dermatophyte microsporum canis was investigated under experimental laboratory conditions. about 400 4-day-old m. domestica flies were divided into two groups. group a consisted of about 200 infected flies and group b comprised about 200 uninfected flies that were used as controls. each trial was run three times. flies from group a were fed for 24 h with a solution of ultra-high temperature-treated (uht) milk containing about 10(6) colony-fo ...200919239611
changing face of tinea capitis in europe.the aim of this review is to update the latest epidemiological situation in europe, explore recent issues in recognition and emerging opportunities in diagnosis and look at progressions in treatment. papers reviewed have, in the main, been published within the last 2 years.200919262376
thymoquinone inhibits germination of dermatophyte arthrospores. 200919271082
influence of different oils on penetration of human hair by fungi.synopsis the hair perforating ability of microsporum gypseum and trichophyton vanbreuseghemii was tested in presence of seventeen oils. clove, olive and turpentine oils were found to be fully inhibitory for hair penetration by both fungi. hair segments smeared with cantheridine oil and keocarpin hair vitalizer failed to reveal any perforation by t. vanbreuseghemii whereas arnica and shikakai oils showed little perforation by this fungus. résumé la capacité de pénétration du microsporum gypseum e ...199319272114
sertaconazole: a review of its use in the management of superficial mycoses in dermatology and gynaecology.sertaconazole (dermofix, ertaczo, ginedermofix, monazol, mykosert or zalain), an imidazole antifungal agent, inhibits the synthesis of ergosterol, an essential cell wall component of fungi. it is indicated in the eu for the treatment of superficial skin mycoses such as dermatophytosis (including tinea corporis, tinea cruris, tinea manus, tinea barbae and tinea pedis), cutaneous candidiasis, pityriasis versicolor and seborrhoeic dermatitis of the scalp, and in the us for tinea pedis only. sertaco ...200919275277
design of conformationally constrained azole antifungals: efficient synthesis and antifungal activity of trans-3-imidazolylflavanones.an efficient protocol has been developed for the preparation of a series of trans-3-imidazolylflavanones and (z)-trans-3-imidazolylflavanone oximes, as potential antifungal agents, by the reaction of 2-imidazolyl-2'-hydroxyacetophenone with different benzaldehyde derivatives and subsequence oximation reaction. the stereochemical and conformational aspects of compounds were also assigned by (1)h nmr spectroscopy. the aryl group present at c-2 and the imidazole ring present at c-3 were assigned to ...200919291101
dermatophyte species in superficial mycoses in the kraków district, poland in the years 1972-2007.considerable changes in the dermatophyte spectrum have been observed in the past century. hence, many authors point out the necessity of performing periodical overviews of the mycological flora producing mycoses in humans in a given area. analysis of dermatophyte species was performed, which were isolated from the lesions in patients suspected of superficial mycosis and referred to the department of mycology. the materials were isolated from patients suspected of superficial mycosis from kraków ...201019298357
leptoglycin: a new glycine/leucine-rich antimicrobial peptide isolated from the skin secretion of the south american frog leptodactylus pentadactylus (leptodactylidae).antimicrobial peptides are components of innate immunity that is the first-line defense against invading pathogens for a wide range of organisms. here, we describe the isolation, biological characterization and amino acid sequencing of a novel neutral glycine/leucine-rich antimicrobial peptide from skin secretion of leptodactylus pentadactylus named leptoglycin. the amino acid sequence of the peptide purified by rp-hplc (c(18) column) was deduced by mass spectrometric de novo sequencing and conf ...200919298834
tinea capitis among children in the columbus area, ohio, usa.tinea capitis is a fungal infection of the hair follicles of the scalp. in the us, the most common organisms have traditionally been trichophyton tonsurans, and occasionally microsporum canis. this study was designed to examine patterns of organisms causing tinea capitis and determine factors associated with infection. a retrospective database analysis was conducted to locate records of patients with tinea capitis from may 2001 to may 2006 at nationwide children's hospital in columbus, oh. descr ...201019302461
antioxidant and antidermatophytic activities of essential oil and extracts of metasequoia glyptostroboides miki ex hu.this study was undertaken to assess the antioxidant and antidermatophytic potential of the essential oil and extracts (hexane, chloroform, ethyl acetate and methanol) of metasequoia glyptostroboides miki ex hu. antioxidant activity was evaluated by using 1,1-diphenyl-2-picrylhydrazyl (dpph) assay. the free radical scavenging activities of the oil and ethyl acetate extract were found to be superior (ic(50)=9.1 and 14.24 microg/ml, respectively) as compared to butylatedhydroxyanisole (bha), (ic(50 ...200919303043
production of peptide antifungal antibiotic and biocontrol activity of bacillus subtilis.among different bacterial cultures, a potent bacillus subtilis mtcc-8114 was isolated from garden soil samples which showed 16 and 14 mm inhibition zones by spot inoculation method and 24 and 22 mm inhibition zones by well agar diffusion method against test fungi i.e. microsporum fulvum and trichophyton species. among four media tested, the maximum growth and antibiotic production was found in trypticase soya broth (tsb) medium at 37 degrees c, ph-7 and 48 h of incubation. the rf value (0.64) by ...200919317353
clinico-etiologic correlates of onychomycosis in sikkim.the etiological spectrum of any superficial mycosis is largely dependent on the flora in the immediate environment of the individual. it is influenced by the geographic, climatic and occupational factors. the study is basically to formulate baseline data for the species prevalence of various dermatophytes in patients with onychomycosis attending the central referral hospital, gangtok, sikkim.200919332910
antimicrobial dihydroisocoumarins from crassocephalum biafrae.bioassay-guided fractionation of the chcl (3)-soluble extract of the stem bark of crassocephalum biafrae (asteraceae) resulted in the isolation of three new dihydroisocoumarins, named biafraecoumarins a ( 1), b ( 2), and c ( 3); two known triterpenes ( 4 and 5); and a known ceramide ( 6). the structures of the new compounds were established as 7-but-15-enyl-6,8-dihydroxy-3( r)-penta-9,11-dienylisochroman-1-one ( 1), 7-butyl-6,8-dihydroxy-3( r)-penta-9,11-dienylisochroman-1-one ( 2), and 7-butyl- ...200919350487
diagnosis: dermatophytid reaction (id reaction). commentary. 200919367834
growth, keratinolytic proteinase activity and thermotolerance of dermatophytes associated with alopecia in uyo, nigeria.mycological research was conducted on the mycelial growth, keratinolytic proteinase activity and thermotolerance ofdermatophytes associated with alopecia patients in uyo, nigeria. the results revealed that microsporum sp. - ap1, epidermophyton sp. - ap2, trichophyton rubrum - ap4, trichophyton mentagrophytes - ap5 and a yeast candida albicans - ap3 isolated exhibited variable growth and keratinase activity at different temperatures. microsporum sp. - ap1 and t. mentagrophytes - ap5 survived heat ...200919388557
heat resistance of dermatophyte's conidiospores from athletes kits stored in nigerian university sport's center.the incidence and heat resistance of conidiospores produced by dermatophytes isolated from athlete's kits (canvasses, stockings and spike shoes) stored in nigerian university sport's centre were investigated. epidermophyton floccosum, microsporum oudouinii, microsporum canis, trichophyton concentricum, trichophyton mentagrophytes and trichophyton rubrum were isolated and their incidence on the athlete's kits varied with the species and type of kits. among the isolates t. mentagrophytes, t. rubru ...200919388558
canine nodular dermatophytosis (kerion): 23 cases.dermatophytosis is a common zoonotic disease, and one of its clinical presentations in the dog is nodular dermatophytosis (kerion). because the infection is located within the dermis, routine diagnostic tests such as a wood's lamp examination, microscopic examination of hair shafts for fungal elements and fungal culture can yield negative results. in such cases, histopathological examination with routine and special stains (periodic acid-schiff, gomori methenamine silver) is required to confirm ...200919392766
antifungal potential of essential oil and various organic extracts of nandina domestica thunb. against skin infectious fungal pathogens.this study was undertaken to assess the in vitro antifungal potential of the essential oil and n-hexane, chloroform, ethyl acetate, and methanol extracts of nandina domestica thunb. against dermatophytes, the casual agents of superficial infections in animals and human beings. the oil (1,000 microg/disc) and extracts (1,500 microg/disc) revealed 31.1-68.6% and 19.2-55.1% antidermatophytic effect against trichophyton rubrum kctc 6345, t. rubrum kctc 6375, t. rubrum kctc 6352, trichophyton mentagr ...200919415265
antimicrobial activity of acacia mellifera extracts and lupane triterpenes.acacia mellifera (vahl) benth (leguminosae) is a subtropical medicinal plant that is widely used in traditional african medicines against various diseases such as pneumonia and malaria.200919429353
nb-002, a novel nanoemulsion with broad antifungal activity against dermatophytes, other filamentous fungi, and candida albicans.nb-002 is an oil-in-water emulsion designed for use for the treatment of skin, hair, and nail infections. the activity of nb-002 was compared to the activities of the available antifungal drugs against the major dermatophytes responsible for cutaneous infections, trichophyton rubrum, trichophyton mentagrophytes, epidermophyton floccosum, and microsporum spp., as well as 12 other genera of filamentous fungi. nb-002 consistently displayed fungicidal activity against all dermatophytes. the comparat ...200919433562
antimicrobial activity of the crude extract, fractions and compounds from stem bark of ficus ovata (moraceae).this study was designed to investigate the antimicrobial activities of the methanol extracts from the stem bark of ficus ovata (fob), fractions (fob1-6) and compounds isolated following bio-guided fractionation [3-friedelanone (1), taraxeryl acetate (2), betulinic acid (3), oleanoïc acid (4), 2-hydroxyisoprunetin (5), 6,7-(2-isopropenyl furo)-5,2,4-trihydroxyisoflavone (6), cajanin (7) and protocatechuic acid (8)].200919450673
tinea capitis in early infancy treated with itraconazole: a pilot study.tinea capitis is the most common fungal infection of the scalp in childhood, but a very rare disorder in the first year of life.200919453785
recent dermatophyte divergence revealed by comparative and phylogenetic analysis of mitochondrial genomes.dermatophytes are fungi that cause superficial infections of the skin, hair, and nails. they are the most common agents of fungal infections worldwide. dermatophytic fungi constitute three genera, trichophyton, epidermophyton, and microsporum, and the evolutionary relationships between these genera are epidemiologically important. mitochondria are considered to be of monophyletic origin and mitochondrial sequences offer many advantages for phylogenetic studies. however, only one complete dermato ...200919457268
epidemiology of fungal scalp infections in the west of scotland 2000-2006.fungal infections of the scalp commonly affect the paediatric population and are caused by dermatophytes which have the ability to invade the keratinised structures of skin, hair and nails. this study analyses the changes in the epidemiology of fungal scalp infections in the west of scotland during the period 2000-2006.200919530495
tinea corporis caused by an unusual strain of microsporum audouinii that perforates hair in vitro.we report on a dermatophyte infection acquired by a young woman from germany who had worked in ghana. the strain isolated from her skin lesions showed morphological and physiological features compatible with microsporum audouinii but a clearly positive hair perforation test made its definite identification by conventional methods equivocal. a genetic analysis finally unambiguously revealed microsporum audouinii. this is the first observation of a microsporum audouinii strain with a positive hair ...201019538519
causative agents of superficial mycoses in istanbul, turkey: retrospective study.the aim of the present study was to determine the percentage of agents, which can give rise to superficial fungal infections in istanbul, turkey. between 2000 and 2007, the clinical samples collected from 8,200 patients attending the outpatient dermatology clinic at mihrimahsultan medical center were examined by direct microscopy and culture. pathogen fungi were detected in 5,722 of the patients. of the isolates were 4,218 (74%) dermatophytes, 1,196 (21%) candida sp., 170 (3%) malassezia furfur, ...200919544086
antifungal activity of bis-azasqualenes, inhibitors of oxidosqualene cyclase.the antifungal activity and in vitro toxicity toward animal cells of two inhibitors of oxidosqualene cyclase, squalene bis-diethylamine (sbd) and squalene bis-diethylmethylammonium iodide (sbdi) were studied. minimum inhibitory concentration (mic) against dermatophytes and other fungi involved in cutaneous and systemic infections (12 isolates from seven species) were determined by the broth microdilution method based on the reference documents m38-a and m27-a2 of clinical and laboratory standard ...201019549106
ultrastructural changes on clinical isolates of trichophyton rubrum, trichophyton mentagrophytes, and microsporum gypseum caused by solanum chrysotrichum saponin sc-2.worldwide, dermatophytoses represent a high percentage of all superficial mycoses. the most frequently isolated dermatophyte is trichophyton rubrum. solanum chrysotrichum is a vegetal species widely used in mexican traditional medicine to treat skin infections; its extract has been used to formulate an herbal medicinal product that is used successfully to treat tinea pedis. spirostanic saponin sc-2 from s. chrysotrichum possesses high activity against dermatophytes. the present study reports the ...200919551614
survey of keratinophilic fungi in sewage sludge from wastewater treatment plants of mazandaran, islamic republic of iran.to isolate keratinophilic fungi in sewage sludge from wastewater treatment plants in sari city, mazandaran province, islamic republic of iran, samples were taken from 7 plants with different sewage treatment technologies. from 35 sludge samples cultured on sabouraud's agar with cycloheximide and chloramphenicol, 326 fungal colonies belonging to 7 species were isolated. geotrichum (59.5%), cladosporium (13.8%), alternaria (11.3%) and penicillium (10.7%) species were the most prevalent. no growth ...200919554993
antifungal and marker effects of talisia esculenta lectin on microsporum canis in vitro.the purpose of this study was to demonstrate the usefulness of lectin obtained from talisia esculenta (tel) seeds as a tool to recognize and study microsporum canis. for this purpose, we investigated the antifungal and marker action of this lectin and the relationship of these effects with the presence of carbohydrates on the structure of this fungus.200919558469
antimicrobial activity of hymenaea martiana towards dermatophytes and cryptococcus neoformans.the biological activity of crude extract and fractions of hymenaea martiana was evaluated against a panel of human pathogenic fungi. the crude extracts and hydroalcoholic fractions (e) showed a high activity against cryptococcus neoformans species complex isolates with mics between 2 and 64 μg ml(-1). the methanolic (c) and butanolic (d) fractions were the most active against trichopyton rubrum, trichopyton mentagrophytes and microsporum canis with mics between 8 and 256 μg ml(-1). none of the e ...201019563478
a 20-year survey of tinea faciei.tinea faciei is an uncommon dermatophytosis affecting the glabrous skin of the face. between 1988 and 2007 at the dermatology department of cagliari university, 107 cases of tinea faciei have been diagnosed, involving 72 females and 35 males, aged 2-72 years. incidence peaks were observed between 6 and 15 years (48.59%) and between 36 and 45 years (17.76%). males below and females above 15 years of age were the most affected. in 61 patients (57.1%), typical forms of tinea faciei were observed, w ...201019563490
[tinea of the eyebrows. about six cases].tinea of the eyebrows is a part of tinea capitis. m. canis, t. violaceum, t. verrucosum and t. mentagrophytes are the most frequently implicated fungi in tinea of the eyebrows.200919569609
dermatophytes, related keratinophilic and opportunistic fungi in indoor dust of houses and hospitals.dermatophytes, related keratinophilic and opportunistic fungi were isolated from indoor dust samples of 46 hospitals and 47 houses in kanpur. a total of 19 fungi represented by 11 genera were isolated by the hair-baiting technique from 230 and 235 samples from hospitals and houses respectively. the isolated fungi are acremonium implicatum (indian type culture collection) itcc 5266, a. strictum (germplasm centre for keratinophilic fungi) gpck 1137, aphanoascus fulvescens gpck 1081, arthroderma si ...200919584506
in vitro activity of tachyplesin iii alone and in combination with terbinafine against clinical isolates of dermatophytes.aim of our study was to investigate the in vitro effects of tachyplesin iii (tp), a potent disulfide-linked peptide, in dermatophytes infections, with respect to or in combination with terbinafine (terb), against 20 clinical isolates of dermatophytes belonging to four species. a broth microdilution method following the clsi recommendations (m38-a) was used for testing drugs alone and in combination. terb mics were significantly lower than those observed for tp (p<0.001). testing for antifungal a ...200919591887
evaluation of a single-tube real-time pcr for detection and identification of 11 dermatophyte species in clinical material.we developed a dermatophyte-specific single-tube real-time pcr assay based on internal transcribed sequences. this assay allows the rapid detection and identification of 11 clinically relevant species within the three dermatophyte genera trichophyton, microsporum and epidermophyton in nail, skin and hair samples within a few hours. analysis of 145 clinical samples (107 nail, 36 skin scale, and two hair) by both real-time pcr and a pcr-reverse line blot (pcr-rlb) assay described earlier revealed ...201019624518
development of transformation system for trichophyton rubrum by electroporation of germinated conidia.dermatophytes are the fungi that can cause infections of skin, hair, and nails due to their ability to utilize keratin. the genetic transformation systems of dermatophytes were successfully applied to trichophyton mentagrophytes and microsporum canis. here we describe the procedure for genetic transformation of trichophyton rubrum by electroporation of their germinated conidia. a linearized transformation vector (pchsh75-pch/gfp/ttrpc) containing bacterial hygromycin b phosphotransferase gene (h ...200919629488
incidence of dermatophytosis in a public hospital of são bernardo do campo, são paulo state, brazil.the incidence of cases of dermatophytosis in dermatology outpatient departments has increased in recent years. infection control is essential to prevent transmission, and accurate diagnosis of this type of infection is important to avoid confusion with other dermatological processes caused by non-fungal agents. the objective of this study was to determine the incidence of dermatophytosis and its etiological agents in a public hospital of são bernardo do campo, brazil. from february 2005 to may 2 ...200919631161
[microsporum canis on dermatologically healthy cats in temuco city, chile].microsporum canis is the most common cause of feline dermatophytosis and the most pathogenic fungus isolated from the skin and hair of healthy cats. cats are considered to be the natural reservoir and infection sourse of this disease in human and domestic animals.200919635446
antioxidant and antidermatophytic activities of essential oil and extracts of magnolia liliflora desr.this study was carried out to assess the antioxidant and antidermatophytic activities of the essential oil and extracts of magnolia liliflora desr. antioxidant activity was evaluated by using 1,1-diphenyl-2-picrylhydrazyl (dpph) assay. the free radical scavenging activities of the oil and ethyl acetate extract were found to be superior (ic(50) values=10.11 and 16.17 microg/ml, respectively) as compared to butylatedhydreoxyanisole (bha), (ic(50) value=18.27 microg/ml). also the ethyl acetate extr ...200919635519
distribution of dermatophytes and other related fungi in jaipur city, with particular reference to soil ph.screening of 217 soil samples of different habitats, such as pg study centre, garden, farmhouse, nursery, roadside, hostel, animal habitat, bird habitat, marriage garden, temple, vegetable market and house dust, was carried out for the presence of dermatophytes and related fungi in relation to soil ph. a total of 461 isolates belonging to 26 genera and 34 species were recorded. soil ph values vary from 3 to 10.5. trichophyton verrucosum, microsporum audouinii and m. canis were isolated for the f ...201119638000
tinea capitis mimicking folliculitis decalvans.we report on an adult patient with tinea capitis caused by microsporum canis, who presented with diffuse alopecia and follicular pustules, mimicking folliculitis decalvans. examination of the scalp showed severe alopecia with prominent involvement of the frontal and vertex scalp: the skin was markedly erythematous with pustules and brownish crusts. videodermoscopy revealed visible follicular ostia, numerous pustular lesions and several comma hairs. fluconazole 150 mg a week for 8 weeks associate ...201119638002
case of kerion celsi caused by microsporum gypseum (arthroderma gypseum ) in a child.we report a case of kerion celsi caused by microsporum gypseum and present some epidemiological statistics and a distribution of the mating types of m. gypseum . a 10-year-old healthy boy living in narita, chiba prefecture, visited the narita red cross hospital in october 2004 with complaints of a scaly erythematous plaque and alopecia. before the visit, he had been treated with steroid lotions and antibiotics without success. a direct examination of the diseased hair shaft using a potassium hyd ...200919654448
[summarization of tinea capitis cases encountered at a clinic in the past 5 years].twelve cases of tinea capitis were encountered at a clinic in kumamoto prefecture between april 2004 and december 2008. patients were boys aged between 2 and 18 in 11 cases, and a woman aged 50 years in 1 case. the pathogen was microsporum canis in 6 cases, and trichophyton tonsurans in 6 cases. in the cases with m. canis , patients were boys aged 2 to 8 years in 5 cases, and the type of disease was non-inflammatory in 5 cases and inflammatory in 1 case. the patients with t. tonsurans were boys ...200919654449
in vitro response of cutaneous mycosis fungal agents to the most widely used systemic antifungals in dermatology.the high frequency of cutaneous mycosis justify the need to evaluate the possible contribution of in vitro profile of susceptibility to antifungal agents.200919668938
[infection with a rare geophilic dermatophyte].there are 12 dermatologically relevant microsporum (m.) species. the most frequent species are m. canis, m. audouinii and m. gypseum. we report an infection of the right forearm with the rare dermatophyte m. fulvum. a koh examination of scales revealed a tinea corporis. the scales were cultured on dermasel(r) agar with the identification of the geophilic dermatophyte m. gypseum. however, its sequencing and mass spectrometry revealed m. fulvum as the correct pathogen.201019680615
comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods.the purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (mic) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species trichophyton rubrum, trichophyton mentagrophytes and microsporum canis. the percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, ...200919684970
extensive neonatal dermatophytoses.we report a 25-day-old boy who was referred to our dermatology unit for evaluation of extensive annular erythematous lesions on his body. we initially considered the differential diagnoses of candidiasis and neonatal lupus erythematosus but investigations revealed the case as tinea corporis due to a relatively uncommon causative agent, microsporum gypseum. to the best of our knowledge it is the first case of extensive neonatal dermatophytoses caused by this organism. the possible causes and the ...201019686303
tinea capitis caused by trichophyton soudanense and microsporum audouinii in an adult: a case report.this report presents a rare case of tinea capitis caused by trichophyton soudanense and microsporum audouinii in a 31-year-old woman from senegal. the patient showed atrophic skin lesions causing cicatricial alopecia, scarring being caused by two aetiological agents uncommon in spain.201119712077
prevalence of tinea pedis, tinea unguium of toenails and tinea capitis in school children from barcelona.to evaluate the prevalence of tinea capitis, tinea pedis, and tinea unguium in children from several schools of barcelona city.200919766517
rp-hplc analysis of rhinacanthins in rhinacanthus nasutus: validation and application for the preparation of rhinacanthin high-yielding extract.a reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of rhinacanthin-c, rhinacanthin-d, and rhinacanthin-n in rhinacanthus nasutus leaves. the method involved the use of a tsk-gel ods-80ts column (5 microm, 4.6 x 150 mm i.d.) with the mixture of methanol and 5% aqueous acetic acid (80:20, v/v) as the mobile phase. the parameters of linearity, repeatability, accuracy, and specificity of the method were evaluated. the recovery of the metho ...200919772749
evaluation of internal transcribed spacer 2-rflp analysis for the identification of dermatophytes.a total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. arthroderma (15 species), chrysosporium (two), epidermophyton (one), microsporum (three) and trichophyton (12), were studied using internal transcribed spacer 2 (its2)-pcr-rflp analysis (its2-rflp), consisting of amplification of the its2 region, restriction digestion with bstui (cg/cg) and restriction fragment length determination by capillary electrophoresis. its2-rflp analysis proved to be most useful for identi ...201019779030
direct detection of five common dermatophyte species in clinical samples using a rapid and sensitive 24-h pcr-elisa technique open to protocol transfer.identification of dermatophytes is usually based on morphological characteristics determined by time-consuming microscopic and cultural examinations. an effective pcr-elisa method has been developed for rapid detection of dermatophyte species directly from clinical specimens within 24 h. isolated genomic dna of skin scrapings and nail samples from patients with suspected dermatophyte infections is amplified with species-specific digoxigenin-labelled primers targeting the topoisomerase ii gene. t ...201119793206
binding of dc-hil to dermatophytic fungi induces tyrosine phosphorylation and potentiates antigen presenting cell function.apcs express receptors recognizing microbes and regulating immune responses by binding to corresponding ligands on immune cells. having discovered a novel inhibitory pathway triggered by ligation of dc-hil on apc to a heparin/heparan sulfate-like saccharide of syndecan-4 on activated t cells, we posited dc-hil can recognize microbial pathogens in a similar manner. we showed soluble recombinant dc-hil to bind the dermatophytes trichophyton rubrum and microsporum audouinii, but not several bacteri ...200919794069
lactone derivatives from the marine-derived fungus penicillium sp. psu-f44.two new fungal metabolites, penicipyrone (1), and penicilactone (2), were isolated from the marine-derived fungus penicillium sp. psu-f44 along with three known macrolides, (+)-brefeldin a (3), (+)-brefeldin c (4), and 7-oxobrefeldin a (5). their antimicrobial activities against methicillin-resistant staphylococcus aureus sk1 and microsporum gypseum sh-mu-4 were examined.200919801865
outbreak of ringworm in a traditional iberian pig farm in spain.an outbreak of dermatophytosis caused by microsporum nanum in a traditional iberian extensive farm is described. the morbidity was 100% among lactating sows; however, suckling and weaning pigs, as well as boars never developed the lesions seen in the sows. the clinical aspects of porcine ringworm caused by this fungus are discussed and the ecology of the organism is reviewed.201119821905
intraspecific variation and interspecific differences in the bacterial and fungal assemblages of blue tit (cyanistes caeruleus) and great tit (parus major) nests.although interest in the relationship between birds and microorganisms is increasing, few studies have compared nest microbial assemblages in wild passerines to determine variation within and between species. culturing microorganisms from blue tit (cyanistes caeruleus) and great tit (parus major) nests from the same study site demonstrated diverse microbial communities with 32 bacterial and 13 fungal species being isolated. dominant bacteria were pseudomonas fluorescens, pseudomonas putida, and ...201019830477
antifungal activity of eugenia umbelliflora against dermatophytes.antifungal activities of eugenia umbelliflora berg. (myrtaceae) were tested in vitro against a panel of standard and clinical isolates of human fungal pathogens (dermatophytes and opportunistic saprobes). methanol extracts of leaves and fruits of e. umbelliflora were separately prepared and partitioned, to yield dichloromethane (dcm), ethyl acetate (etoac) and aqueous fractions (aq). three compounds (1-3) were obtained from the dcm extract using chromatographic procedures. antifungal assays were ...200919831024
majocchi's granuloma caused by microsporum canis in an immunocompetent patient. 201019832861
molecular characterization of selected dermatophytes and their identification by electrophoretic mutation scanning.dermatophytes are fungi that can be contagious and cause infections in the keratinized skin of mammals, including humans. the etiological diagnosis of dermatophytosis relies on a combination of in vitro-culture and microscopic methods. effective molecular tools could overcome the limitations of conventional methods of identification. in the present study, following phenetic identification as m. canis, m. fulvum, m. gypseum, t. mentagrophytes and t. terrestre, we genetically characterized key der ...200919862737
prevalence of tinea capitis in southeastern austria between 1985 and 2008: up-to-date picture of the current situation.tinea capitis is the most common dermatophyte infection in childhood, but may rarely occur in adults and the elderly. causative agents vary within different geographical areas as well as during decades. the aim of this study was to evaluate the prevalence and causative agents of tinea capitis in southeastern austria. retrospective analysis of 714 patients diagnosed with tinea capitis seen at the outpatient department of dermatology/medical university of graz during the time period 1985-2008 was ...201119863725
organization and evolutionary trajectory of the mating type (mat) locus in dermatophyte and dimorphic fungal pathogens.sexual reproduction in fungi is governed by a specialized genomic region, the mating type (mat) locus, whose gene identity, organization, and complexity are diverse. we identified the mat locus of five dermatophyte fungal pathogens (microsporum gypseum, microsporum canis, trichophyton equinum, trichophyton rubrum, and trichophyton tonsurans) and a dimorphic fungus, paracoccidioides brasiliensis, and performed phylogenetic analyses. the identified mat locus idiomorphs of m. gypseum control cell t ...201019880755
comparison of the activities of four antifungal agents in an in vitro model of dermatophyte nail infection.onychomycosis is a difficult condition to treat and cure rates are disappointing. moreover fungicidal action of antifungal agents in nccls assays and their rapid accumulation in nails in vivo are not compatible with the duration of treatment.200819882010
dynamics of dermatophytosis frequency in mexico: an analysis of 2084 cases.we analysed 15,101 biological samples from patients presenting with superficial mycoses who attended outpatient services over a 10-year period. scale samples were processed for direct microscopic examination with 15% koh and cultured on sabouraud glucose agar plus chloramphenicol and cycloheximide. laboratory examination confirmed 4,709 cases of superficial mycosis (31.18%), of which 2,084 (44.26%) were dermatophytoses. the species most frequently encountered was trichophyton rubrum (71.2%), fol ...201019886762
diagnostic pcr tests for microsporum audouinii, m. canis and trichophyton infections.since traditional diagnosis of dermatophyte infections is slow, we present a rapid new pcr test for detection of trichophyton spp., microsporum canis and m. audouinii infections. the performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human dna control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from guinea pigs experimentally infected with m. canis; and two samples from un-infected control animals. dna ...201019886764
evaluation of a pcr melting profile method for intraspecies differentiation of trichophyton rubrum and trichophyton interdigitale.in order to identify the source of infections caused by dermatophytes, as well as the pathogen transmission pathway, there is a need to determine methods that allow detailed genetic differentiation of the strains within the dermatophyte genera. in this work, a pcr melting profile (pcr-mp) technique based on the ligation of adaptors and the difference in melting temperatures of dna restriction fragments was used for the first time for intraspecies genotyping of dermatophytes. clinical isolates an ...201019892858
use of western blot to study microsporum canis antigenic proteins in canine dermatophytosis.western blotting was used to describe the microsporum canis proteins with antigenic activity in dogs with dermatophytosis. electrophoretic separation of whole fungal strain extract cultured from a cat was performed under denaturing conditions. the proteins were blotted onto nitrocellulose and probed with sera collected from 22 dogs with dermatophytosis (18 m. canis, 3 m. gypseum, 1 trichophyton mentagrophytes; group a), 20 dogs with skin diseases other than dermatophytosis, and 22 dogs with no c ...201119912544
argentinean propolis from zuccagnia punctata cav. (caesalpinieae) exudates: phytochemical characterization and antifungal activity.this paper reports the in vitro antifungal activity of propolis extracts from the province of tucuman (argentina) as well as the identification of their main antifungal compounds and botanical origin. the antifungal activity was determined by the microdilution technique, using reference microorganisms and clinical isolates. all dermatophytes and yeasts tested were strongly inhibited by different propolis extracts (mics between 16 and 125 microg ml(-1)). the most susceptible species were microspo ...201019916546
the spectrum of fungal infections with a special reference to dermatophytoses in the capital area of kuwait during 2000-2005: a retrospective analysis.fungal infections constitute a major health problem all over the world. signs and symptoms induced by various dermatophytic infections are difficult to distinguish clinically from each other. so, characterization by in vitro culture is required for appropriate diagnosis and treatment as well as to study the epidemiological characteristics in a region.201019921463
a 5-year survey of dermatomycoses in southwest poland, years 2003-2007.the study was performed to analyse the spectrum of dermatomycoses in southwest poland during the period 2003-2007. a total of 10,486 patients were investigated for fungal skin infections by means of native specimen and cultivating procedures. skin scrapings, plucked hairs and nail clippings were examined and identified by direct microscopy and culture. from 2468 patients, 2753 fungi were identified including dermatophytes, yeast and moulds. among the dermatophytes, the most common pathogen isola ...201119925570
tinea capitis in southeastern china: a 16-year survey.this survey was a retrospective of a 16-year (1993-2008) study on the incidence, clinical features, and etiological agents of tinea capitis mainly representing the southeastern china. the diagnosis was confirmed by direct microscopic examination. eight hundred and sixty-six patients with tinea capitis, 381 males (44%) and 485 females (56%), were enrolled in this study. patients were between 20 days and 84 years old with an average of 10.5 years and the peak incidence was in the age group of 6-10 ...201019936963
determination of antifungal activity of polyenzyme preparation candidaseb using in vitro methods.a new polyenzyme formulation 'candidaseb' was tested for its ability to inhibit clinically important fungi. after its antifungal activity was established, an assay was standardized to determine whether any potentiation effect was possible if candidaseb was used in conjunction with three standard antifungal agents viz, amphotericin b, miconazole and ketoconazole against 5 clinically significant fungi viz., candida albicans, cryptococcus neoformans, aspergillusniger, microsporum gypseum and tricho ...200719957538
cd4+ and cd8+ t cells mediated direct cytotoxic effect against trichophyton rubrum and trichophyton mentagrophytes.the cellular immune system is the most dominant factor in curing acute dermatophytosis. however, the exact immune mechanisms involved in generating this defense are complex and still obscure. the aim of this study was to investigate the fungicidal mechanism of t cells in the normal population versus patients with chronic fungal infections.201019968718
secreted subtilisin sub3 from microsporum canis is required for adherence to but not for invasion of the epidermis.microsporum canis is a pathogenic dermatophyte that causes a superficial cutaneous mycosis, mainly in cats and humans. proteolytic enzymes, including subtilisins, have been postulated to be key factors involved in adherence and invasion of the stratum corneum and keratinized epidermal structures.201019995373
tinea capitis: still an unsolved problem?tinea capitis is a fungal infection specifically involving the scalp and hair. it is the most common dermatophyte infection in children under 12 years of age, with a predominance in those of sub-saharan african descent. common signs include hair loss, scaling, erythema and impetigo-like plaques. adults may also be affected, but to a lesser degree. the causative species are from the microsporum and trichophyton genera. limited treatment options and diverse modes of transmission complicate the cli ...201120002884
identification of antibacterial and antifungal pharmacophore sites for potent bacteria and fungi inhibition: indolenyl sulfonamide derivatives.synthesis of seven new indolenyl sulfonamides, have been prepared by the condensation reaction of indole-3-carboxaldehyde with different sulfonamides such as, sulphanilamide, sulfaguanidine, sulfathiazole, sulfamethoxazole, sulfisoxazole, sulfadiazine and sulfamethazine. these synthesized compounds have been used as potential ligands for complexation with some selective divalent transition metal ions (cobalt, copper, nickel & zinc). structure of the synthesized ligands has been deduced from thei ...201020005022
composition and biological activity of the essential oil from leaves of plinia cerrocampanensis, a new source of alpha-bisabolol.the essential oil from fresh leaves of plinia cerrocampanensis barrie (myrtaceae), obtained by hydrodistillation, was analysed by gc-fid and gc-ms. forty components, representing more than 91% of the oil, were identified. oxygenated sesquiterpenes represented the main fraction with alpha-bisabolol (42.8%) as the major constituent, making this plant a new and good source of this substance. biological activity of the essential oil was evaluated against several bacterial and fungal strains as well ...201020015638
synthesis, structure, and antifungal evaluation of some novel 1,2,4-triazolylmercaptoacetylthiosemicarbazide and 1,2,4-triazolylmercaptomethyl-1,3,4-thiadiazole analogs.novel 1-[[4-(4-bromophenyl)-5-(2-furyl)-4h-1,2,4-triazole-3-yl]mercaptoacetyl]-4-alkyl/aryl-3-thiosemicarbazides (5-12) were synthesized by the reaction of 4-(4-bromophenyl)-5-(2-furyl)-4h-1,2,4-triazole-3-ylmercaptoacetylhydrazide (4) with substituted isothiocyanates. cyclodehydration of thiosemicarbazides with concentrated sulfuric acid yielded 2-[4-(4-bromophenyl)-5-(2-furyl)-4h-1,2,4-triazole-3-yl]mercaptomethyl-5-alkyl/arylamino-1,3, 4-thiadiazoles (13-17). the new compounds were evaluated ...201020030516
in vitro antimicrobial activity of four ficus carica latex fractions against resistant human pathogens (antimicrobial activity of ficus carica latex).methanolic, hexanoïc, chloroformic and ethyl acetate extracts of ficus carica latex were investigated for their in vitro antimicrobial proprieties against five bacteria species and seven strains of fungi. the green fruit latex was collected from chott mariam souse, middle east coast of tunisia. the antimicrobial activity of the extracts was evaluated and based respectively on the inhibition zone using the disc-diffusion assay, minimal inhibition concentration (mic) for bacterial testing and the ...201020067867
circumscribed inflammatory plaque at the scalp: follicular impetigo or tinea capitis? 201020084590
steroidal glycosides from the underground parts of allium ursinum l. and their cytostatic and antimicrobial activity.the aim of this study was the isolation and structural elucidation of steroidal glycosides from the underground parts of ramson allium ursinum l. the structures of the isolated compounds were established based upon chromatographic methods and 1d- and 2d-nmr, ms and ir analyses. the mixture of two steroidal saponins: (25r)-spirost-5-en-3b-ol tetrasaccharide and (25r)-spirost-5, 25(27)-dien-3b-ol tetrasaccharide, along with a 3-hydroxypregna-5,16-dien-20-one glycoside were identified. the results ...200620085228
synthesis and in vitro microbiological evaluation of an array of biolabile 2-morpholino-n-(4,6-diarylpyrimidin-2-yl)acetamides.biolabile 2-morpholino-n-(4,6-diarylpyrimidin-2-yl)acetamides 34-42 have been synthesized and evaluated for their in vitro antibacterial and antifungal activities. the minimum inhibitory concentration tested for the same compounds against the same set of bacterial and fungal strains shows that the compounds 36 and 38 against beta-heamolytic streptococcus and klebsiella pneumonia, 40 against escherichia coli and pseudomonas, have excellent antibacterial activity. compounds 36, 38 and 42 show inhi ...201020106563
recovery of dermatophytes in pet grooming tools from veterinary clinics and pet grooming salons.fomites such as brushes and clippers are known to be potential risk factors for the transmission of dermatophytes between pet animals. the aim of this study was to investigate this risk by examining those grooming implements for the presence of any viable dermatophytes.201020137008
cutaneous mycoflora and cd4:cd8 ratio of cats infected with feline immunodeficiency virus.this study was designed to compare cutaneous mycoflora isolation and cd4+:cd8+ ratio in feline immunodeficiency virus (fiv)-infected cats with that in fiv-uninfected cats. sixty cats were examined. twenty-five were fiv-infected cats and 35 were fiv-uninfected cats. all 60 cats were felv-negative. fungi were speciated and immunophenotyping of peripheral cd4+ and cd8+ t lymphocytes was performed. at least one fungal colony was isolated from 22/25 (88%) fiv-infected cats. among the fiv-uninfected c ...201020156698
antimicrobial activity of pentacyclic triterpenes isolated from acacia mellifera.acacia mellifera has been used widely in traditional african medicines against various diseases. among the kipsigis community of kenya, water extracts from the plant is used for the treatment of skin diseases, coughs and gastrointestinal ailments. the aim of the study was to provide scientific rationale for the use of the plant in traditional medicine through bioassay-guided fractionation of a. mellifera stem bark. bioactivity testing was done against selected microbes using disc diffusion techn ...200820162040
efficacy of local herbal therapy in the management of dermatophytosis among primary school children in cross river state, south-south nigeria.as a contribution to the on-going search for alternative, available and affordable treatment of common infections in sub-saharan africa, the efficacy of local herbs, senna alata(linn) and borreria ocymoides (burm), in comparison with conventional drugs, griseofulvin and clotrimazole in the treatment of dermatophytosis among primary school children, was examined in the three districts of cross river state, south-south nigeria. out of 840 pupils screened, 68 (8.1%) were infected, with incidence ra ...200920175416
chemical constituents from the leaves of boehmeria rugulosa with antidiabetic and antimicrobial activities.three new flavonoid glycosides, named chalcone-6'-hydroxy-2',3,4-trimethoxy-4'-o-beta-d-glucopyranoside (1), isoflavone-3',4',5,6-tetrahydroxy-7-o-[beta-d-glucopyranosyl-(1-->3)-alpha-l-rhamnopyranoside] (2), and isoflavone-3',4',5,6-tetrahydroxy-7-o-[beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl-(1-->3)-alpha-l-rhamnopyranoside] (3), were isolated from the leaves of boehmeria rugulosa, together with five known compounds, beta-sitosterol, quercetin, 3,4-dimeth ...200920183275
synthesis and antifungal activity of 1-aryl-3-phenethylamino-1-propanone hydrochlorides and 3-aroyl-4-aryl-1-phenethyl-4-piperidinols.mono-mannich bases, 1-aryl-3-phenethylamino-1-propanone hydrochlorides, 1a, 2a, 3a, 4a, 5a, 6a, 7a, 8a, 9a, and semi-cyclic mono-mannich bases, 3-aroyl-4-aryl-1-phenethyl-4-piperidinols, 1b, 2b, 3b, 4b, 5b, 6b, 7b, 8b, 9b, were synthesized by a non-classical mannich reaction. the aryl part was: c(6)h(5 )for 1a, 1b; 4-ch(3)c(6)h(4) for 2a, 2b; 4-ch(3)oc(6)h(4 )for 3a, 3b; 4-clc(6)h(4 )for 4a, 4b; 4-fc(6)h(4) for 5a, 5b; 4-brc(6)h(4) for 6a, 6b; 2,4-(cl)(2)c(6)h(3) for 7a, 7b; 4-no(2)c(6)h(4 )for ...201020232370
does this young girl have the same rash as her mother? 201020232720
synthesis and antileishmanial and antimicrobial activities of some 2,3-disubstituted 3h-quinazolin-4-ones.a series of 2,3-disubstituted 3h-quinazolin-4-ones was synthesized. antimicrobial activities of the synthesized compounds were investigated against gram (+ve) and gram (-ve) bacteria, including b. subtilis, s. aureus, s. flexneri, p. aeruginosa, and s. typhi, and six fungi, namely trichophyton longifusus, candida albicans, aspergillus flavus, microsporum canis, fusarium solani, and candida glabrata using the broth microdilution method. compounds 9, 11, and 12 showed significant activities agains ...201020235747
tinea capitis due to microsporon audouini (three cases). 194720283710
microsporum audouini; the effect of yeast extract, thiamine, pyridoxine, and bacillus weidmaniensis on the colony characteristics and macroconidial formation. 194720290993
epidemiology and risk factors for dermatophytoses in rabbit farms.while dermatophytoses of several animal species have been extensively investigated, information on their occurrence and epidemiology in rabbits is limited. we carried out a study from october 2006 to february 2007 of 23 rabbit farms in apulia and basilicata regions (southern italy) in order to investigate the occurrence and risk factors associated with dermatophytoses in breeding rabbits. dermatophytes were isolated from 86.9% (20/23) of the farms and from 51.8% (420/810) of the animals sampled. ...201020297949
a study of the effects of long wave x-rays on the staphylococcus aureus and on the microsporum audouini. 192920317327
[tinea capitis: two years experience in a paediatric hospital of buenos aires, argentina].tinea capitis is an infection caused by dermatophytes of the genera microsporum and trichophyton, and constitutes a major health problem in argentina. the aim of the present study was to find out the incidence of those etiological agents and the therapeutic response in patients attending a high-complexity paediatric hospital within a two-year period. a total of 98 tinea capitis were diagnosed, 13 of which were celsus kerion. microsporum canis was isolated in 61.28%. the range of values for minim ...201020346290
disease progression of dermatophytic pseudomycetoma in a persian cat.dermatophytic pseudomycetoma is a subcutaneous fungal infection by microsporum canis.201020346294
a zoonotic ringworm outbreak caused by a dysgonic strain of microsporum canis from stray cats.cats are frequent carriers of microsporum canis and veterinary students are at high risk of exposure and acquisition of the organism a la infección.201020346301
historical aspects of dermatomycoses.physicians have been aware of superficial fungal infections for centuries, but the causal agents and treatments of fungal infections remained unknown until the mid-1800s, when numerous important findings were reported. among the relevant researchers in the field of superficial mycoses were remak, who found the fungal nature of favus in 1837; berg, who reported oral candidosis in 1841; and wilkinson, who described vaginal candidosis in 1849. tinea versicolor was described clinically in 1846 by ei ...201020347652
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