Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| multiple sclerosis (second of two parts). | 1982 | 6290885 | |
| membrane changes during viral infection. | the effect of viruses on the surface membrane of susceptible cells during the entry and exit process has been studied. haemolytic paramyxoviruses induce a non-specific leakage to low-molecular-weight compounds during entry; other viruses do not show this effect. during exit, no such changes occur with any virus so far studied: some viruses are released without any obvious change at all in surface membrane function; in other cases, uptake of some nutrients is altered and there is a fall in membra ... | 1982 | 6291665 |
| microfilaments associated with paget's disease of bone: comparison with nucleocapsids of measles virus and respiratory syncytial virus. | the structural characteristics and intracellular disposition of microtubules present in osteoclasts in paget's disease of bone were compared with those of nucleocapsids in cells infected with measles virus and respiratory syncytial virus (rsv). the distribution of microtubules in pagetic osteoclasts closely paralleled that of measles nucleocapsids in monkey kidney cells, but the dimensions of the pagetic microtubules were significantly different from those of measles virus. in contrast, pagetic ... | 1982 | 6292129 |
| [a rapid method for the determination of virus-specific igm antibodies]. | a newly developed ion exchange chromatography is reported; it is a simple and quick method to separate a highly purified igm fraction from blood serum. the immunofluorescence test was used to demonstrate that these fractions contain only virus-specific igm antibodies, but no demonstrable igg antibodies, which can often block the specific igm reactivity by competitive inhibition. the specificity of the reaction was tested on sera from 145 persons: the igm test was always positive in clinically pr ... | 1982 | 6293973 |
| different virus antibodies in serum and cerebrospinal fluid of patients suffering from subacute sclerosing panencephalitis. | complement fixing (cf) antibody titers to measles, parainfluenza (pi) types 1 and 3, mumps, herpes type 1, and cytomegalovirus (cmv) in serum and cerebrospinal fluid (csf) of 33 patients with subacute sclerosing panencephalitis (sspe) were evaluated. results were analyzed in comparison to 11 patients with neurological diseases other than sspe and 7 normal subjects. all sspe patients had elevated serum and csf measles antibody titers. the number of sspe patients manifesting elevated titers other ... | 1982 | 6296199 |
| postembedding immunocytochemical localization of paramyxovirus antigens by light and electron microscopy. | a postembedding method is described to localize antigens specific for various paramyxoviruses in sections of cells and tissues that have been fixed and embedded in epoxy resins for conventional electron microscopy. viral antigens were localized in cv-1 cell cultures infected with simian virus 5 (sv5), brains of suckling hamsters inoculated with either neuroadapted mumps virus or hamster-adapted measles virus, and brains of adult mice infected with sendai (parainfluenza i) virus. both 1-micromete ... | 1982 | 6296222 |
| hotting-up the complement-fixation test. | a detailed investigation into the effect of modifying the incubation temperature of the complement-fixation (cf) test is described. for varicella-zoster virus cytomegalovirus and rubella virus, increasing the incubation temperature progressively increased the sensitivity of the cf test to reach a maximum at 15 degrees c, at which temperature the geometric mean titre of seropositive samples was significantly greater than that found at 4 degrees c. for these three viruses, each serum shown to cont ... | 1983 | 6296229 |
| [cellular immunity study of multiple sclerosis in the lymphocyte blast transformation reaction to the viruses of acute human encephalomyelitis, herpes and measles]. | the blast transformation test revealed a high level of lymphocyte sensitization to human acute encephalomyelitis virus in 12 out of 29 (41.9%) patients with multiple sclerosis in the early stages of the disease and in the period of exacerbation in patients with long-term disease. the pattern of the blast transformation test in response to herpes simplex and measles viruses did not depend on the duration of the disease. high, moderate, and low levels of lymphocyte blast transformation reaction to ... | 1982 | 6297170 |
| simultaneous primary infections with epstein-barr virus and measles virus in fatal acute encephalitis. | 1983 | 6297658 | |
| an investigation of the antiviral activity of podophyllum peltatum. | the antiviral activity of an aqueous extract of podophyllum peltatum was investigated. the extract was fractionated by reversed-phase chromatography, and podophyllotoxin was found to be the most active component in inhibiting the replication of measles and herpes simplex type i viruses. beta-peltatin and desoxypodophyllotoxin produced marginal antiviral effects, while alpha-peltatin and picropodophyllotoxin were inactive at the levels tested. | 1982 | 6298369 |
| specific and non-specific b cell activation in measles and varicella. | lymphocytes from eight patients with measles and six patients with varicella were studied during the acute phase (first week) of illness and after recovery for spontaneous and pokeweed mitogen (pwm)-induced production of immunoglobulins (ig) and viral antibodies by an enzyme linked immunosorbent assay (elisa). in both infections acute phase lymphocytes showed increased spontaneous in vitro igm and igg productions including igm and igg antibodies to the aetiological virus as well as igg antibodie ... | 1983 | 6299636 |
| molecular mimicry in virus infection: crossreaction of measles virus phosphoprotein or of herpes simplex virus protein with human intermediate filaments. | using monoclonal antibodies, we demonstrate that the phosphoprotein of measles virus and a protein of herpes simplex virus type 1 crossreact with an intermediate filament protein of human cells. this intermediate filament protein, probably vimentin, has a molecular weight of 52,000, whereas the molecular weights of the measles viral phosphoprotein and the herpes virus protein are 70,000 and 146,000, respectively. crossreactivity was shown by immunofluorescent staining of infected and uninfected ... | 1983 | 6300911 |
| lymphocyte adherence in multiple sclerosis: lack of virus specificity. | the virus specificity of adherence of peripheral blood mononuclear leukocytes from patients with multiple sclerosis and from age- and sex-matched healthy volunteers to tissue culture cells infected with measles virus, newcastle disease virus, and vesicular stomatitis virus was studied. lymphocyte adherence to uninfected cells is uniformly low (5-15% tissue culture cells with greater than 3 lymphocytes adhered). adherence to cells infected with virus is enhanced 2- to 4-fold in controls and 2- to ... | 1983 | 6303601 |
| inactivation of measles and herpes simplex viruses by trypan blue. | the dye trypan blue inhibits infection of cells in tissue culture by measles and herpes simplex viruses at concentrations in excess of 0.01% (w/v) principally by direct inactivation of the virions. the inactivation is temperature-dependent (10(4) times greater at 37 degrees c than at 4 degrees c) and its mechanism appears to include inhibition of virus adsorption to cells. | 1983 | 6304237 |
| effect of antibody-induced modulation of measles (sspe) virus membrane proteins on beta-adrenergic receptor-mediated adenylate cyclase activity. | the effect of measles virus antiserum on the expression of viral glycoproteins on the membranes of measles (sspe) persistently infected c6 rat glioma cells was studied. there was a gradual loss of virus membrane antigen from these cells until no antigen was detectable 18 days after initiation of antiserum treatment. at this stage approximately 25% of cells still displayed intracellular virus antigen which was also lost after further cell passages. there was an accompanying recovery of the previo ... | 1983 | 6306911 |
| hybridoma cell lines secreting monoclonal antibodies against foot-and-mouth disease virus. 1. cell culturing requirements. | the production of hybridoma cell lines secreting antibody against foot-and-mouth disease virus (fmdv) was more difficult than the production of similar cell lines secreting antibody against vesicular stomatitis virus or measles virus. a rapid and efficient protocol for the selection and culturing of 'anti-fmdv' hybridoma cultures was therefore developed and is described. this required the determination of the optimal culture medium (commercially available), source of serum supplement, line of my ... | 1983 | 6309848 |
| amphotericin b selection of mutant chinese hamster cells with defects in the receptor-mediated endocytosis of low density lipoprotein and cholesterol biosynthesis. | this paper describes a rapid and efficient two-step procedure for the isolation of mutant cells with defects in receptor-mediated endocytosis. the procedure takes advantage of two fungal metabolites, compactin (ml236b), a potent inhibitor of cholesterol biosynthesis, and amphotericin b, a polyene antibiotic that forms toxic complexes with sterols in membranes. mutagen-treated chinese hamster ovary cells were preincubated overnight in a medium containing mevalonate, low density lipoprotein (ldl), ... | 1983 | 6310583 |
| sera from irradiated rats contain antibodies to a ubiquitous tumour-associated antigen. | female rats of the inbred strains bn/birij and wag/rij were irradiated with 30 kv x-rays, or 15 mev or 0.5 mev fast neutrons. sera were collected several months after irradiation and found to be negative for antibodies reacting with the murine mammary tumour virus as tested by a solid-phase radioimmunoassay and an immunofluorescence absorption test. we found, however, that in an immunofluorescence assay several sera from irradiated rats reacted with a cytoplasmic antigen in rat mammary, ureter a ... | 1983 | 6311562 |
| [antibodies to viruses in children with diabetes mellitus]. | serological studies were carried out in the time course of insulin-dependent diabetes mellitus in 419 children, among whom paired sera from 66 were studied in the very beginning of diabetes mellitus. by seroconversion in 83% of the children early in the disease, different, frequently mixed virus infections were diagnosed: coxsackie b2, b3, b4 (46%), rubella (41%), influenza a, b, c (38%), parainfluenza types 1-3 (35%), mumps (23%), adenovirus infection (18%), hb virus infection (4.5%). acute res ... | 1983 | 6314665 |
| myxoviruses do not induce non-specific alterations in membrane permeability early on in infection. | the permeability characteristics of cells infected with myxoviruses have been studied by measuring the concentrative uptake of nutrients, the concentration of intracellular k+, and the maintenance of the na+ gradient across the plasma membrane. cells either show no change at all (sendai virus-infected bhk cells and measles virus-infected vero cells) or they show a decreased ability to concentrate nutrients, while intracellular k+ and the na+ gradient remain unchanged (sendai and influenza virus- ... | 1983 | 6314933 |
| [bronchiolitis obliterans: technics of exploration and diagnosis]. | bronchiolitis obliterans is an anatomo-clinical entity which may, in young pediatric patients, follow severe viral infections, especially due to measles virus and adenoviruses. after a transient remission, the disease progresses to chronic respiratory failure with dyspneic paroxysms. diagnosis is based on different investigations which confirm the absence of proximal bronchiolar obstruction and the presence of bronchiolar obliteration. deficient pulmonary perfusion due to ventilatory defects are ... | 1983 | 6316524 |
| comparison of the viral proteins of canine parvovirus-2, mink enteritis virus and feline panleukopenia virus. | canine parvovirus-2 (cpv-2), mink enteritis virus (mev) and feline panleukopenia virus (fpv) were produced using identical cell culture and purification techniques. the distributions of the haemagglutinating activity of the three different parvoviruses in a cscl gradient were similar with haemagglutinating peaks identified at 1.48-1.49, 1.42, 1.36 and 1.30-1.31 g cm-3. the number and distribution of the viral proteins and the equivalent protein molecular weights are similar for all three viruses ... | 1983 | 6316627 |
| is pregnancy immunosuppressive? humoral immunity against viruses. | serum samples were obtained during the first trimester, second trimester, third trimester, at delivery and 6 weeks postpartum from each of 50 pregnant women. all 250 sera were tested for their content of antibodies specific for herpes simplex, measles, rubella and influenza a viruses. the geometric mean titres of antiviral antibody were shown to decline by 18-48% between the times of booking and delivery, and to return to initial values by the end of the puerperium. by means of two-way analysis ... | 1983 | 6317008 |
| [the viral hypothesis of schizophrenia. results of virological research]. | 1983 | 6317208 | |
| multiple sclerosis: subclasses of intrathecally synthesized igg and measles and varicella zoster virus igg antibodies. | paired samples of serum and concentrated cerebrospinal fluid (csf) from 20 multiple sclerosis (ms) patients and 10 controls with no central nervous system disease were electrofocused and analysed for igg and measles and varicella zoster virus igg antibodies by imprint immunofixation using igg subclass specific monoclonal antibodies. all but one of the ms patients had intrathecally synthesized bands of oligoclonal igg in the csf. in fifteen of the ms patients the oligoclonal igg bands were restri ... | 1983 | 6317243 |
| [epidemiological study on the assay of antibodies to anti-torch agents and to hbv in a group of mother/child pairs in palermo]. | the torch group: toxoplasma, rubeola, cytomegalovirus (cmv), herpes virus hominis (hsv) and also the b-virus of hepatitis (hbv) determine congenital malformations in the newborns. for this reason, the authors leaded an epidemiologic study testing with some antibodies (anti-torch and anti-hbv) the serum of groups of mother-newborn of the obstetrical clinic of palermo. this study became manageable because now there are available high specific immunoenzymatic methods, for example elisa, the passive ... | 1983 | 6322822 |
| measles virus: conditions for the propagation and purification of infectious virus in high yield. | tissue culture conditions for the efficient propagation of cell-free measles virus, and a novel method for the purification of infectious virus in milligram quantities are described in this report. infected suspension cultures of hela cells incubated at 32.5 degrees c yielded virus titers approaching 10(8) pfu/ml, 30-50% of which was cell-free. after concentration by ultrafiltration and sedimentation, infectious virus was separated from host cell membranes and proteins by density equilibrium cen ... | 1984 | 6323505 |
| is there an indigenous viral flora in man? | 1984 | 6325702 | |
| oligoclonal igg bands in the cerebrospinal fluid of monkeys with experimental allergic encephalomyelitis. | eae in monkeys resulted in csf ocb appearing 2 to 3 weeks p.i., with bp and complete freund's adjuvant. clinical signs appeared close to the time that the bands were first detected. the animals had serum measles antibodies, however, the titers did not change during the development of eae and no measles antibodies were detected in the csf. | 1984 | 6326160 |
| immune balance in the cytomegalovirus-infected host: in vitro studies of virus-lymphocyte interactions and the effects on specific lymphocyte function. | 1984 | 6329358 | |
| [immunological effectiveness of a booster inoculation against measles in children remaining seronegative after vaccination]. | booster immunization against measles with a highly immunogenic vaccine leads to the development of prolonged postvaccinal immunity lasting at least 6-7 years (the term of observation) in the groups of children found to be seronegative after the titration of their blood sera with 1 hemagglutinating unit (hau) of the antigen. the booster immunization of children in whose blood sera the minimal concentrations of antibodies can be determined in the presence of 1 hau of the antigen (seronegative in t ... | 1984 | 6333122 |
| decreased interferon synthesis and responsiveness to interferon by leukocytes from multiple sclerosis patients given natural alpha interferon. | despite the use of interferon (ifn) in numerous clinical trials, relatively little is known about how ifn therapy influences leukocyte function. to evaluate some of its effects, leukocytes from multiple sclerosis (ms) patients given daily treatments of natural alpha ifn (ifn-alpha) were evaluated for ifn synthesis and two responses to ifn in vitro: enhancement (priming) of ifn synthesis and suppression of concanavalin a (con a)-induced t-cell mitogenesis. the ifn therapy had no effect on the sen ... | 1984 | 6333472 |
| cell mediated immunity in patients with subacute sclerosing panencephalitis. | cell mediated immunity was assessed on the basis of total lymphocytes (tl), total t lymphocytes (ttl) counts, delayed skin test responses and in vitro leucocyte migration inhibition test (lif) production in 25 patients with sspe, classified according to the clinical stages of the disease. the patients in stage i of the disease did not show any defect in cellular immunity while the patients in stage ii showed decreased tl and ttl counts, more negative skin test responses to pha, sksd and ppd, and ... | 1984 | 6333831 |
| measles virus variants: viral replication and cytopathogenicity in human t lymphocytes and b lymphoblastoid cells. | measles virus can replicate in circulating t cells, b cell and monocytes of man as well as in different human lymphoblastoid cell lines. in the present report we describe the effects on lymphoblastoid cells and on a defined population of human t lymphocytes of infection with three measles virus variants characterized by different cytopathogenicity in human and simian epithelial cell lines. the aim of the investigation was to establish some evidence that the evolution of the viral infection and t ... | 1984 | 6334219 |
| subacute sclerosing panencephalitis. | sspe is a rare, late manifestation of measles-virus infection. discussed are its clinical features, laboratory findings, pathology, epidemiology, pathogenesis, and treatment. | 1984 | 6334226 |
| antibody response to measles immunization in india. | antibody response to measles vaccine was measured in 238 subjects aged 6-15 months. seroconversion rates ranged from 74% at 6 months of age to 100% at 13-15 months; the differences in age-specific rates were not statistically significant. the postimmunization antibody titres increased with increasing age of the vaccinee. seroconversion rates and antibody titres in 49 subjects with grades i and ii malnutrition were not significantly different from those in the 189 normal subjects.these results su ... | 1984 | 6334571 |
| characterization of a non-syncytiogenic autonomously replicating variant of measles virus. | a variant, derived from both measles and subacute sclerosing panencephalitis virus preparations, incapable of producing intercellular fusion or syncytia typical of measles virus infections is described. this non-syncytiogenic (n-s) virus was easily detected using immunofluorescence, was genetically stable and replicated autonomously. the variant was defective in the production of both infectious virus and haemagglutinin in vero and hep2 cells. persistent infections in hep2 cells were readily est ... | 1983 | 6338153 |
| biosynthesis of measles virus hemagglutinin in persistently infected cells. | the synthesis of the hemagglutinin (ha) glycoprotein of measles virus was investigated in a persistently infected cell line using a monoclonal anti-ha. the synthesis of the ha protein was shown to be associated with the rough endoplasmic reticulum. the unglycosylated (ha0) apoprotein is synthesized as a 65,000 dalton peptide and is inserted into the rough endoplasmic reticulum as a transmembrane protein with approximately 2 to 3000 daltons of the peptide exposed to the cytoplasmic membrane surfa ... | 1983 | 6338870 |
| successful immunization of children with and without maternal antibody by aerosolized measles vaccine. i. different results with undiluted human diploid cell and chick embryo fibroblast vaccines. | inhalation of undiluted, aerosolized measles vaccine was immunogenic in 100% of 4- to 6-month-old and older children with and without residual maternal antibody when the human diploid cell (hdc) vaccine containing the ikić (edmonston-zagreb) strain and 1% human albumin was used but in a smaller percentage of infants given a chick embryo fibroblast (cef) vaccine, which contained the edmonston-schwarz strain, ten times more virus, and hypertonic sugar solution but no added protein. prevaccination ... | 1983 | 6341638 |
| discriminant analysis of data in enzyme immunoassay. | a critical step in the development of both qualitative and quantitative enzyme immunoassays is establishing the positive/negative discrimination, or cut-off, value. data derived from an indirect immunofluorescence assay, hemagglutination inhibition, and enzyme immunoassay to detect igg antibodies to measles virus were applied to a discriminant analysis program to determine the positive/negative cut-off value. application of the discriminant analysis demonstrated a greater utilization of the sens ... | 1983 | 6343498 |
| a new solid-phase enzyme-linked immunosorbent assay for specific antibodies to measles virus. | a convenient and flexible enzyme-linked immunosorbent assay system for the detection of specific antibodies to measles virus has been developed. in this system infected cells are desiccated on 96-well microtiter plates and stored at room temperature. after incubation of samples to be tested in the cell-coated plates and subsequent washing, bound antibodies are detected with a peroxidase-conjugated staphylococcal protein a probe. after another washing and the addition of the appropriate substrate ... | 1983 | 6343523 |
| the proteins of morbilliviruses. | 1983 | 6343552 | |
| detection of igm antibodies to measles virus by enzyme-immunoassay. | a method of solid-phase enzyme-immunoassay (eia) with horseradish-peroxidase-conjugated anti-mu-globulin was used to determine igm antibodies to measles virus in human sera. antigens prepared from measles-infected and noninfected vero cells passively adsorbed to polystyrene cuvettes were both important for the tests, because sera from many patients convalescing from viral infections and sera from rheumatoid arthritis patients were able to bind to control antigen. sera from measles patients, pati ... | 1983 | 6343817 |
| comparison of morbillivirus proteins by limited proteolysis. | proteins of a number of measles and sspe virus strains have been compared by limited proteolysis and they appear to be largely conserved amongst the various strains. viruses derived from sspe cannot be distinguished from other measles viruses by this technique. small differences in the digest patterns of the m proteins have been observed between the edmonston and other measles virus strains. furthermore, in some strains where the m proteins migrate slower in sds-page the limited proteolysis patt ... | 1983 | 6343818 |
| fate of microfilaments in vero cells infected with measles virus and herpes simplex virus type 1. | in herpes simplex virus type 1-infected vero cells, reorganization of microfilaments was observed approximately 4 h postinfection. conversion of f (filamentous) actin to g (globular) actin, as assessed by a dnase i inhibition assay, was continuous over the next 12 to 16 h, at which time a level of g actin of about twice that observed in uninfected cells was measured. fluorescent localization of f actin, using 7-nitrobenz-2-oxa-1,3-diazole (nbd)-phallacidin, demonstrated that microfilament fibers ... | 1983 | 6343843 |
| a fourteen-year study to identify measles antigen in urine specimens by fluorescent-antibody methods. | between 1965 and 1979 inclusive, 426 morning urine specimens from 410 patients admitted to hospital with the provisional diagnosis of measles were tested for measles antigen by fluorescent-antibody methods. one hundred and forty-two specimens (33.3 per cent) were positive. of 74 patients who were clinically assessed not to have measles, 6/77 specimens (7.8 per cent) gave false positive results, and 71/77 (92.2 per cent) gave true negative results. three hundred and forty-nine urine specimens wer ... | 1983 | 6348178 |
| measles immunization in iran. | mass immunization with live measles vaccine has been carried out in rural iran since 1968. two strains have been used: primary baby calf kidney cell-adapted sugiyama strain and human diploid cell-adapted aik strain. more than 94% of the susceptible children experimentally vaccinated with either of the two vaccines have shown seroconversion. mass immunization in rural regions has covered about 80% of susceptible children. it is now recommended that the live vaccine be administered twice: the firs ... | 1983 | 6348917 |
| immunization against measles by aerosol. | about 20 years ago, japanese and american investigators found that inhalation of relatively small amounts of aerosolized, partly attenuated measles vaccines was consistently immunogenic in nearly all susceptible children. the resulting modified measles was not transmissible to susceptible children. in 1965-1966, japanese investigators showed that levels of neutralizing antibody produced by killed measles virus vaccine, which prevented subsequent immunization when live vaccine was given subcutane ... | 1983 | 6348918 |
| evidence for a viral etiology of paget's disease of bone. | ultrastructural studies of the bone cells in patients with paget's disease of bone have revealed the presence of nuclear and cytoplasmic inclusions that resemble the nucleocapsids of paramyxoviridae virus. immunocytologic studies of bone biopsy specimens and cultured bone cells have demonstrated positive responses with antisera against measles virus and/or respiratory syncytial virus. these observations suggest that paget's disease may be a slow viral infection of bone. the exact nature of the p ... | 1983 | 6349897 |
| antibody assay for measles virus: comparisons of immune-adherence haemagglutination, single radial haemolysis, enzyme immunoassay and haemagglutination inhibition. | immune-adherence haemagglutination (iaha); single radial haemolysis and enzyme immunoassay (elisa) versus haemagglutination inhibition (hai) for detecting antibodies to measles virus were evaluated. the rank correlation computed according to spearman for the sera of healthy individuals gave values of 0.66, 0.73, 0.72, respectively, for hai-iaha, hai-single radial haemolysis (srh) and hai-elisa. high percentages of accordance were observed as regards sera from both healthy and vaccinated individu ... | 1983 | 6350336 |
| characterization of four cell lines persistently infected with measles virus. | persistently infected cell lines were established by infecting vero cells with four different strains of measles virus: edmonston "wild type", schwarz vaccine strain passaged at high multiplicity of infection, hallé sspe strain, and a temperature sensitive mutant of edmonston strain, designated ts 841. the four cell lines have continued to produce virus at a constant low level over a period of more than two years, although cytopathology and hemagglutinating ability have varied with cell passage. ... | 1983 | 6354137 |
| [bacterial adsorption on cultivated mammalian cells in conditions of mixed infection]. | the method based on the use of 3h-labeled bacteria was applied to study the relationship between the magnitude of uropathogenic e. coli strain adsorption on mammalian cells in vitro and preinfection of the latter ones with virus. the presence of measles virus in cell culture raised the adsorption level of bacteria. meanwhile oncoviruses did not alter the adsorption level. the possibility of generalized alterations of the cell surface by virus infection is discussed. | 1983 | 6354301 |
| use of monoclonal antibodies to human immunoglobulin m in "capture" assays for measles and rubella immunoglobulin m. | monoclonal antibodies to human immunoglobulin m (igm) were used in a four-phase enzyme immunofluorescence "capture" assay for determination of igm antibodies to measles and rubella viruses. little or no background reactivity was seen in the test system, and interfering effects of rheumatoid factor were avoided by preabsorption of test sera with aggregated human igg. virus-specific igm antibody was demonstrable in 23 of 24 patients with serological evidence of measles virus infections and in 36 o ... | 1983 | 6355153 |
| sensitivity and reproducibility of enzyme immunoassay for measles virus antibody detection. | the immunoenzymatic elisa method has been applied to the detection of antibody to measles virus by using a single serum dilution. the reproducibility has been tested over three groups of sera negative, intermediate and strongly positive, in 48 different assays. the standard deviations and the variation coefficients are reported which show very good reproducibility both within and between runs. as regard sensitivity elisa has proved more sensitive than haemagglutination inhibition (hai) by testin ... | 1983 | 6355776 |
| evidence for both respiratory syncytial virus and measles virus antigens in the osteoclasts of patients with paget's disease of bone. | recent ultrastructural and immunohistochemical evidence supports the hypothesis that paget's disease of bone is a slow viral infection of the paramyxoviridae family. conflicting evidence for the presence of respiratory syncytial virus (rsv), a pneumovirus, or measles, a morbillivirus, has been reported. by the indirect fluorescent antibody assay, four rsv antisera were compared with four measles antisera on serial sections of pagetic bone or replicate coverslips of cells from pagetic bone grown ... | 1984 | 6365389 |
| biological activity of a monoclonal antibody to a measles virus haemagglutinin epitope detected late in infection. | a hybrid cell line secreting monoclonal antibody 2h9c (mab 2h9c) with measles virus haemagglutinin (ha) specificity was produced. the mab 2h9c was non-reactive in haemagglutination inhibition and neutralization assays. a protein of apparent mol. wt. 79000 was immune-precipitated using a triton x-100/sds/sodium deoxycholate detergent buffer and two proteins with mol. wt. of 79000 and 69000 were immune-precipitated using 1% nonidet p40/sodium deoxycholate buffer in sds-page assays. similar results ... | 1984 | 6366120 |
| [subacute sclerosing panencephalitis]. | 1983 | 6367219 | |
| successful immunization of infants with and without maternal antibody by aerosolized measles vaccine. ii. vaccine comparisons and evidence for multiple antibody response. | in 4- and 5-month-old infants in whom the undiluted chick embryo fibroblast (cef) schwarz strain measles vaccine had a poor immunogenic effect, there was an increase in immunogenicity when the high sugar concentration was diminished without reference to added albumin. the human diploid cell (hdc) measles vaccine was still superior in this age group even in a lower concentration of the ikić, edmonston-zagreb strain of virus. more aerosolized, hdc ikić strain virus was required for high seroconver ... | 1984 | 6368888 |
| enzyme-linked immunosorbent assay for evaluation of immunity to measles virus. | an enzyme-linked immunosorbent assay for the determination of immunity to measles virus was developed and standardized; it was compared to the hemagglutination inhibition and plaque reduction neutralization methods for sensitivity and specificity. the conditions of the enzyme-linked immunosorbent assay were adjusted such that groups of susceptible and immune individuals were clearly separable on the basis of the reactivity of a single (1:100) dilution of their sera to viral and control antigens. ... | 1984 | 6371044 |
| [measles virus--antigenic structure]. | 1983 | 6372050 | |
| polymorphonuclear leukocyte-associated antigenemia in measles: demonstration and significance. | an indirect immunoperoxidase technique was used for the identification of measles virus (mev) antigen in routinely prepared peripheral blood smears. characteristic brown immunoperoxidase staining was present in the polymorphonuclear (pmn) leukocytes in 25 of 31 children during the exanthematous stage of measles. no peroxidase staining was seen on peripheral blood smears in any of 9 children convalescing from measles or in 9 children with upper respiratory tract infections ( urti ) without exanth ... | 1984 | 6374033 |
| recognition of host-membrane antigens in the envelope of measles virions. | trypsin- and acetone-treated virions from either of two strains of measles virus grown in vero cells stimulated the production in guinea pigs of (i) virus-specific antibodies to polypeptide (p) of molecular weight 70,000 (70k) and to the portion of the ha spike embedded in the viral envelope, but not to m protein, and (ii) antibodies to two host cell membrane antigens which were identified as glycoproteins with molecular weights of 108k and 104k. these host cell antigens were present in increase ... | 1984 | 6376421 |
| [chronic viral infections]. | 1984 | 6376937 | |
| prospects for the eradication of infectious diseases. | the meaning of eradication, which is an irreversible conclusion, is considered primarily to distinguish it from elimination, which is reversible from outside the area. poliomyelitis and measles are at present the diseases for which conditions most favor an attempt to produce eradication. poliomyelitis has now reached a frequency in the developing world as high as it was in the prevaccine era of the united states. the use of oral vaccine is a deliberate attempt to substitute the wild-type poliovi ... | 1984 | 6377445 |
| measles virus persistency and disease. | 1984 | 6379751 | |
| membrane proteins and virus virulence. | 1984 | 6382744 | |
| clinical trial of a new trivalent measles-mumps-rubella vaccine in young children. | trivalent measles-mumps-rubella virus vaccines, containing either schwarz (two dosage levels), urabe am 9, and ra 27/3 strains or moraten, jeryl lynn, and ra 27/3 strains, were evaluated in 174 children aged 14 to 24 months. each of the three vaccines induced a nearly 100% seroconversion for measles and mumps and a full 100% seroconversion for rubella. close home monitoring disclosed a high incidence and a relatively constant pattern of vaccine-associated reactions, similar for all three vaccine ... | 1984 | 6383020 |
| comparison of nonspecific reactivity in indirect and reverse immunoassays for measles and mumps immunoglobulin m antibodies. | serum specimens collected from patients convalescing from acute measles or mumps infections, other viral infections, or rheumatoid arthritis and from blood donors were tested in indirect and reverse assays for measles and mumps immunoglobulin m (igm) antibodies. all the samples from patients convalescing from acute mumps and measles infections gave positive igm results in both tests. however, 6% of sera from patients recovering from other viral infections, 68.4% of sera from patients with rheuma ... | 1984 | 6392329 |
| detection by hybridization of viral infection of the human central nervous system. | 1984 | 6398012 | |
| measles serology in children with a history of measles in early life. | 1983 | 6405849 | |
| intrathecal antibody synthesis to virus antigens in multiple sclerosis. | intrathecal antibody synthesis against 17 common viruses and mycoplasma pneumoniae lipid antigen was measured in 30 multiple sclerosis patients and 30 patients with other neurological diseases. antibody synthesis was found against all of the antigens in at least a few of the ms patients. the highest number of patients had intrathecal synthesis of antibodies to measles, rubella and paramyxo type viruses and the lowest frequency was against m. pneumoniae, herpes simplex virus, adenovirus and cytom ... | 1983 | 6407791 |
| development and characteristics of live aik-c measles virus vaccine: a brief report. | 1983 | 6410489 | |
| [2 topics in the recollection of my research]. | 1983 | 6411946 | |
| measles-virus-persistent infection in bgm cells. modification of the incorporation of [3h]arachidonic acid and [14c]stearic acid into lipids. | in bgm cells chronically infected with measles virus, although the composition of the phospholipids is unaltered, the fatty acid composition is modified. uninfected, lytic and persistently infected cells were labelled with [3h]arachidonic acid and [14c]stearic acid and their metabolic fate analysed. no difference in the total incorporation was observed in the different systems. however, the [14c]stearic acid and [3h]arachidonic acid were incorporated up to 2-fold and 13-fold respectively greater ... | 1983 | 6414459 |
| [virus infection in the central nervous system: immunological abnormalities in sspe]. | 1983 | 6414494 | |
| bone tissue in paget's disease treated by ethane-1, hydroxy-1, 1 diphosphonate (ehdp). structure, ultrastructure, and immunocytology. | diphosphonates have been used in the management of paget's disease of bone because of their experimentally demonstrated antiosteoclastic activity. light microscopic examination of bone tissue from 13 patients with paget's bone disease who were receiving (ehdp) treatment showed considerably reduced osteoclastic resorption accompanied by gradual replacement of irregular woven bone by lamellar tissue. electron microscopic examination confirmed that pagetoid osteoclasts are morphologically abnormal ... | 1984 | 6423333 |
| a non-haemagglutinating isolate of mink enteritis virus. | a virus was isolated from mink showing clinical and pathological signs of mink enteritis. this virus was identified as mink enteritis virus (mev) from results of serological tests, determination of its density in cscl (1.415 g cm-3), and morphology, including size (20 nm in diameter). the isolate was designated mev-s. in contrast to other known mev strains, the mev-s isolate has no haemagglutinating (ha) activity with swine red blood cells (rbcs) at 4 degrees c and ph 6.8. neither was there any ... | 1984 | 6437057 |
| [current problems in measles. ii. measles vaccines--recent progress]. | 1984 | 6438773 | |
| measles virus inhibits acquisition of lymphocyte functions but not established effector functions. | 1980 | 6444548 | |
| interferon as a mediator of human lymphocyte suppression. | evidence is presented that interferon (if) is a major mediator of the human concanavalin a (con a) suppressor cell. the suppressive effects of con a-activated lymphocytes on the mitogen responses of normal responder cells were largely abrogated by addition of anti-human leukocyte if serum. similar suppressor activity was generated by coculture of peripheral blood leukocytes (pbl) with a melanoma cell line (mewo) and a hela cell line persistently infected with measles virus that induced the produ ... | 1980 | 6444662 |
| [antibodies to measles virus among the urban and rural population of the andizhan region]. | 1980 | 6449812 | |
| changes in immune response during responses in ms patients. | changes in clinical status and in two measures of immune function were followed for 21 months (median) in 106 multiple sclerosis (ms) patients and cohabitant controls. antibody titers to measles, cytomegalovirus, and herpesvirus 1 and 2, and leukocyte migration inhibition indexes (lmis) to measles and streptokinase/streptodornase (sksd) were measured at 3- to 6-month intervals and at time of exacerbation in the index case. there were 36 exacerbations in 25 patients. mean baseline antibody titers ... | 1981 | 6452588 |
| [chromosome disorders in the leukocytes of healthy children and children with down's syndrome vaccinated and unvaccinated against measles]. | 1981 | 6454997 | |
| measles virus vaccination of measles seropositive individuals suppresses lymphocyte proliferation and chemotactic factor production. | 1981 | 6459900 | |
| natural killer-interferon system in patients with preleukaemic states. | the natural killer (nk)-interferon (ifn) system was investigated in patients with preleukaemic states. endogenous nk cell activity was markedly reduced in all 12 patients studied. no significant correlation was observed between the activity of nk cells and the percentage of blast cells in the peripheral blood or bone marrow. the frequency of large granular lymphocytes (lgl) in lymphocytes of the peripheral blood was not reduced in most patients. the percentage of target binding cells in patients ... | 1984 | 6466573 |
| [epidemiological characteristics of measles in zhuji joint prevention area]. | 1984 | 6467351 | |
| re-evaluation of immune status to measles, mumps and rubella viruses in the canada health survey population and the indochinese refugee population. | 1984 | 6478370 | |
| induction of acute myoclonic encephalopathy in hamsters by subacute sclerosing panencephalitis virus. | myoclonus is a characteristic neurological sign of subacute sclerosing panencephalitis (sspe). attempts were made to induce myoclonus in a large proportion of hamsters with a cell-associated strain of sspe virus (the biken strain) and thereby to establish an experimental model for study of the mechanism of development of this condition. when injected intracerebrally, biken virus induced myoclonus within two to 14 days in 84% of the three- to nine-week-old hamsters tested. electroencephalographic ... | 1984 | 6481183 |
| [regulation of the excisional repair system in in vitro-cultured human cells by measles viruses depending on their attenuation]. | the effect of measles viruses (attenuated strain l-16 and virulent strain edmonston) was studied in human cells (line l-41) according to the criteria of repair: excision of thymine dimers, repair replication of dna and resynthesis of dna breaks induced by uv-irradiation. reproduction of attenuated measles virus in cells was accompanied with stimulation of excision of thymine dimers and repair replication of dna in contradistinction to mild virus. this phenomenon depended on multiplicity of infec ... | 1984 | 6487787 |
| induction of delayed-type hypersensitivity to measles virus in mice. | intracutaneous injection of inactivated measles virus (mv) into hind footpads of balb/c mice infected 5 to 11 days previously with mv produces a strong delayed-type hypersensitivity (dth) response. pretreatment of mice with cyclophosphamide (cp) results in a significantly stronger response. in cp-pretreated mice, the optimal infecting dose of live mv and the restimulating amount of inactivated mv are approximately 10(7) plaque-forming units and 2 micrograms/mouse, respectively. the optimal time ... | 1984 | 6488331 |
| [efficacy of measles vaccination in young malnourished african children]. | a survey aiming at assessing the anti-measles vaccination efficiency among malnourished children, 1-3 years old, has been conducted in rural upper-volta. the haemagglutination inhibition technique has been used and antibodies titers higher or equal to 20 were considered as markers of protective immunity. malnutrition does not seem to be a factor that decreases the anti-measles antibodies production, because there is no significative difference between seroconversion percentages of well and malno ... | 1984 | 6488419 |
| complement fixing antibodies in patients of suspected subacute sclerosing panencephalitis in delhi. | 1984 | 6490111 | |
| slow development of measles virus (edmonston strain) infection in the brain of nude mice. | the edmonston strain of measles virus caused neurologic disease in athymic nude mice by intracerebral inoculation. the incubation periods of the disease, however, were extremely long, ranging from 59 to 140 days when the mice were inoculated with 10(4) plaque forming units (pfu) of the virus. the edmonston strain was highly infectious in the nude mouse brain since virus infection was established even with 1 pfu of the virus. virus titers in the brains of infected mice increased with the time of ... | 1984 | 6493073 |
| treatment of multiple sclerosis with anti-measles cow colostrum. | previous virological and immunological studies have suggested that multiple sclerosis (ms) is an auto-immune disease triggered by a virus infection. in order to inhibit the growth of measles virus in the patient's jejunum, we obtained an iga-rich cow colostrum containing anti-measles lactoglobulin resistant to proteases. this colostrum was orally administered to patients with ms to investigate its effect on the course of the disease. measles-positive antibody colostrum was orally administered ev ... | 1984 | 6493135 |
| measles virus and inactivated canine distemper virus induce incomplete immunity to canine distemper. | pairs of specific pathogen free dogs were immunized with two injections of heat inactivated canine distemper virus (cdv) or one injection of a live cdv or live measles virus (mv) vaccine. three unimmunized dogs were used as controls. all 9 dogs were challenged with virulent cdv (snyder hill strain). the three unimmunized dogs developed severe signs of disease with a lethal infection in one. the two dogs immunized with live cdv vaccine developed a strong humoral as well as cellular immune respons ... | 1984 | 6497659 |
| [acute neurologic complications of rubella]. | two cases of rubella with neurological complications are reported. in one case an extensive atypical polyradiculoneuritis was associated with a late albumino-cytologic dissociation. the other presented as a transverse myelitis with meningitis and radiculitis. in neither case was an history of contagion or recent vaccination elicited, and no eruption appeared in either patient. clinical onset was sudden, with an acute meningitis followed several days later by neurological deficits. the course was ... | 1984 | 6505495 |
| [humoral immunological reactivity in subacute sclerosing panencephalitis]. | in a group of 37 children with sspe serum protein and cerebrospinal fluid electrophoresis was done (in 34 and 25 cases respectively) determining immunoglobulins in the serum and cerebrospinal fluid in all 37 cases. the obtained results demonstrated a lack of correlation between the changes observed in the serum and cerebrospinal fluid. the most frequent change in the serum was a rise in alpha 2 globulin level (85%) and reduced iga level (58%), while in the cerebrospinal fluid a rise in gamma glo ... | 1984 | 6514115 |
| [comparative characteristics of the physicochemical properties of the vaccinal strain of measles virus l-16 and its moscow-5 clone]. | certain physical and chemical properties of the measles virus vaccine strain l-16 and its clonal variant m-5 proposed as a duplicate of the vaccine strain were studied. the buoyant density of both viruses in sucrose was 1.18-1.19 g/cm3. virions were found to contain 6 structural proteins. electron microscopic examinations revealed no differences in the morphology of virions of the vaccine l-16 strain and m-5 clone. | 1984 | 6528575 |
| measles virus gene expression in subacute sclerosing panencephalitis. | rna was extracted from the diseased brain of a case of human subacute sclerosing panencephalitis (sspe) and analysed for the expression of measles-specific rna. measles virus-specific mrnas were present, but the amount of matrix (m) protein mrna was greatly reduced in comparison to lytically infected cells and phospho- (p) protein mrna was hardly detectable whereas the level of the corresponding intermediate-sized (is-) rna was greatly increased. rna obtained from the human brain was also transl ... | 1984 | 6534032 |
| field trial of a heat-stable measles vaccine in papua new guinea. | in papua new guinea, consideration has been given to the introduction of measles vaccination, and a trial was conducted to determine the optimum age for vaccination and the suitability of a heat-stable measles vaccine (rimevax) for countrywide use. as much of the vaccination programme is carried out by mch teams on foot patrols, the trial was designed to determine immunogenicity of the vaccine after being transported and used for a week in a standard issue vaccine esky by mch staff. a study of t ... | 1984 | 6534989 |