Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| antimicrobial properties of tris(homoleptic) ruthenium(ii) 2-pyridyl-1,2,3-triazole "click" complexes against pathogenic bacteria, including methicillin-resistant staphylococcus aureus (mrsa). | a series of tris(homoleptic) ruthenium(ii) complexes of 2-(1-r-1h-1,2,3-triazol-4-yl)pyridine "click" ligands (r-pytri) with various aliphatic (r = butyl, hexyl, octyl, dodecyl, and hexdecyl) and aromatic (r = phenyl and benzyl) substituents was synthesized in good yields (52%-66%). the [ru(r-pytri)3](2+)(x(-))2 complexes (where x(-) = pf6(-) or cl(-)) were characterized by elemental analysis, high-resolution electrospray ionization mass spectrometry (hr-esi-ms), (1)h and (13)c nuclear magnetic ... | 2016 | 27657170 |
| number of grooming partners is associated with hookworm infection in wild vervet monkeys (chlorocebus aethiops). | there are many known benefits of social grooming among primates, including maintenance of social relationships, removal of ectoparasites, and improved physiological condition. recently, however, researchers have noted that social grooming and social contact may also present a significant cost by facilitating transmission of some parasites and pathogens. we investigated whether the number of social grooming partners varied based on infection status for gastrointestinal parasites. we used focal an ... | 2016 | 27682258 |
| blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: implications for a minimally invasive biomarker of mitochondrial health. | blood based bioenergetic profiling strategies are emerging as potential reporters of systemic mitochondrial function; however, the extent to which these measures reflect the bioenergetic capacity of other tissues is not known. the premise of this work is that highly metabolically active tissues, such as skeletal and cardiac muscle, are susceptible to differences in systemic bioenergetic capacity. therefore, we tested whether the respiratory capacity of blood cells, monocytes and platelets, are r ... | 2016 | 27693859 |
| protective effects of degraded soybean polysaccharides on renal epithelial cells exposed to oxidative damage. | this study aimed to investigate the protective effects of degraded soybean polysaccharides (dsp) on oxidatively damaged african green monkey kidney epithelial (vero) cells. low dsp concentration (10 μg/ml) elicited an evident protective effect on h2o2-induced cell injury (0.3 mmol/l). the cell viabilities of the h2o2-treated group and the dsp-protected group were 57.3% and 93.1%, respectively. the cell viability decreased to 88.3% when the dosage was increased to 100 μg/ml. dsp protected vero ce ... | 2016 | 27701856 |
| male food defence as a by-product of intersexual cooperation in a non-human primate. | males in a number of group-living species fight in intergroup conflicts to defend access to food resources, a seemingly paradoxical behaviour, given that this resource does not usually limit male fitness directly. we investigated the mechanism(s) driving apparent male food defence in wild vervet monkeys (chlorocebus aethiops pygerythrus) by testing the effect that female resource access, and female audience size and activity had on the response of focal males during simulated intergroup encounte ... | 2016 | 27775042 |
| a single amino acid dictates protein kinase r susceptibility to unrelated viral antagonists. | during millions of years of coevolution with their hosts, cytomegaloviruses (cmvs) have succeeded in adapting to overcome host-specific immune defenses, including the protein kinase r (pkr) pathway. consequently, these adaptations may also contribute to the inability of cmvs to cross species barriers. here, we provide evidence that the evolutionary arms race between the antiviral factor pkr and its cmv antagonist trs1 has led to extensive differences in the species-specificity of primate cmv trs ... | 2016 | 27780231 |
| identification of novel kaposi's sarcoma-associated herpesvirus orf50 transcripts: discovery of new rta isoforms with variable transactivation potential. | kaposi's sarcoma-associated herpesvirus (kshv) is a gammaherpesvirus that has been associated with primary effusion lymphoma and multicentric castleman's disease, as well as its namesake kaposi's sarcoma. as a gammaherpesvirus, kshv is able to acutely replicate, enter latency, and reactivate from this latent state. a key protein involved in both acute replication and reactivation from latency is the replication and transcriptional activator (rta) encoded by the gene orf50 rta is a known transact ... | 2017 | 27795414 |
| isolation of a simian immunodeficiency virus from a malbrouck (chlorocebus cynosuros). | to investigate the diversity of simian immunodeficiency virus (siv) among nonhuman primates (nhps) in zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel siv recovered by co-culturing african green monkey (agm) peripheral blood lymphocytes with human cd4(+) t-cell lines. we report the first described siv (sivagmmal-zmb) from a malbrouck (chlorocebus cynosuros). sivagmmal-zmb was detected by real-time pcr analysis of splenic rna in 3.2% (3/94) of ... | 2017 | 27804019 |
| development and evaluation of topotecan loaded solid lipid nanoparticles: a study in cervical cancer cell lines. | the study aims at statistical development of solid lipid nanoparticles (slns) loaded with topotecan hydrochloride for avoiding the drawbacks of conventional drug therapies used in cervical cancer. twenty sln batches were prepared using organic solvent evaporation method to provide response surface curves. thereafter, optimized slns were obtained using numeric method based on desirability functions providing maximum drug loading and appropriate particle size. physical characterization of optimize ... | 2016 | 27816641 |
| phytosynthesized gold nanoparticles from c. roxburghii dc. leaf and their toxic effects on normal and cancer cell lines. | gold nanoparticles are considered of great importance compared to other noble metal nanoparticles and its wide range of applications like pharmaceutics, therapeutics and diagnostics etc. during the past decade, phytosynthesized gold nanoparticles (aunps) are more focused in in vitro and in vivo study. the present study was focused on the gold chloride and phytosynthesized gold nanoparticles from aqueous leaf extract of cassia roxburghii and their toxic effects on african green monkey normal kidn ... | 2016 | 27855358 |
| uterine angioleiomyoma in an african green monkey (chlorocebus aethiops sabaeus). | a uterine neoplasm was observed, as an incidental finding, during post-mortem examination of a 26-year-old female multiparous african green monkey (chlorocebus aethiops sabaeus). the intramural, expansile, 2 to 3 cm well-demarcated, dark-red, nodular neoplasm was located on the anterior uterine body (corpus) wall. | 2017 | 27859319 |
| female monkeys use both the carrot and the stick to promote male participation in intergroup fights. | group-level cooperation often poses a social dilemma in which joint action may be difficult to achieve. theoretical models and experimental work on humans show that social incentives, such as punishment of defectors and rewarding of cooperators, can promote cooperation in groups of unrelated individuals. here, we demonstrate that these processes can operate in a non-human animal species, and be used to effectively promote the production of a public good. we took advantage of the fact that interg ... | 2016 | 27881752 |
| in vivo evaluation of [(18)f]fecimbi-36, an agonist 5-ht2a/2c receptor pet radioligand in nonhuman primate. | we recently reported the radiosynthesis and in vitro evaluation of [(18)f]-2-(4-bromo-2,5-dimethoxyphenyl)-n-(2-(2-fluoroethoxy)benzyl)ethanamine, ([(18)f]fecimbi-36) or ([(18)f]1), an agonist radioligand for 5ht2a/2c receptors in postmortem samples of human brain. herein we describe the in vivo evaluation of [(18)f]fecimbi-36 in vervet/african green monkeys by pet imaging. pet images show that [(18)f]fecimbi-36 penetrates the blood-brain barrier and a low retention of radioactivity is observed ... | 2017 | 27889455 |
| systematic evaluation of monoclonal antibodies and immunoassays for the detection of interferon-γ and interleukin-2 in old and new world non-human primates. | non-human primates (nhp) provide important animal models for studies on immune responses to infections and vaccines. when assessing cellular immunity in nhp, cytokines are almost exclusively analyzed utilizing cross-reactive anti-human antibodies. the functionality of antibodies has to be empirically established for each assay/application as well as nhp species. a rational approach was employed to identify monoclonal antibodies (mab) cross-reactive with many nhp species. panels of new and establ ... | 2017 | 27889562 |
| cxcr6-mediated simian immunodeficiency virus sivagmsab entry into sabaeus african green monkey lymphocytes implicates widespread use of non-ccr5 pathways in natural host infections. | african green monkeys (agm) and sooty mangabeys (sm) are well-studied natural hosts of simian immunodeficiency virus (siv) that do not progress to aids when infected with their species-specific viruses. natural hosts of siv express very low levels of the canonical entry coreceptor ccr5, and recent studies have shown that ccr5 is dispensable for siv infection of sm in vivo and that blocking of ccr5 does not prevent ex vivo infection of peripheral blood mononuclear cells (pbmc) from sm or vervet a ... | 2017 | 27903799 |
| animal models of respiratory syncytial virus infection. | human respiratory syncytial virus (hrsv) is a major cause of respiratory disease and hospitalisation of infants, worldwide, and is also responsible for significant morbidity in adults and excess deaths in the elderly. there is no licensed hrsv vaccine or effective therapeutic agent. however, there are a growing number of hrsv vaccine candidates that have been developed targeting different populations at risk of hrsv infection. animal models of hrsv play an important role in the preclinical testi ... | 2017 | 27908639 |
| neural stem cells derived from human parthenogenetic stem cells engraft and promote recovery in a nonhuman primate model of parkinsons disease. | cell therapy has attracted considerable interest as a promising therapeutic alternative for patients with parkinsons disease (pd). clinical studies have shown that grafted fetal neural tissue can achieve considerable biochemical and clinical improvements in pd. however, the source of fetal tissue grafts is limited and ethically controversial. human parthenogenetic stem cells offer a good alternative because they are derived from unfertilized oocytes without destroying potentially viable human em ... | 2016 | 27931506 |
| short interfering rna inhibits rift valley fever virus replication and degradation of protein kinase r in human cells. | rift valley fever virus (rvfv) is a mosquito-borne zoonotic pathogen causing severe outbreaks in humans and livestock in sub-saharan africa and the arabian peninsula. human infections are characterized by fever, sometimes leading to encephalitis, retinitis, hemorrhagic fever, and occasionally death. there are currently no fully licensed vaccines or effective therapies for human use. gene silencing mediated by double-stranded short interfering rna (sirna) is a sequence-specific, highly conserved ... | 2016 | 27933051 |
| plague vaccine development: current research and future trends. | plague is one of the world's most lethal human diseases caused by yersinia pestis, a gram-negative bacterium. despite overwhelming studies for many years worldwide, there is no safe and effective vaccine against this fatal disease. inhalation of y. pestis bacilli causes pneumonic plague, a fast growing and deadly dangerous disease. f1/lcrv-based vaccines failed to provide adequate protection in african green monkey model in spite of providing protection in mice and cynomolgus macaques. there is ... | 2016 | 28018363 |
| isolation, molecular characterization, and in vitro schizogonic development of sarcocystis sp. ex accipiter cooperii from a naturally infected cooper's hawk (accipiter cooperii). | raptors serve as the definitive host for several sarcocystis species. the complete life cycles of only a few of these sarcocystis species that use birds of prey as definitive hosts have been described. in the present study, sarcocystis species sporocysts were obtained from the intestine of a cooper's hawk (accipiter cooperii) and were used to infect cell cultures of african green monkey kidney cells to isolate a continuous culture and describe asexual stages of the parasite. two clones of the pa ... | 2017 | 28033565 |
| whole genome analysis provides evidence for porcine-to-simian interspecies transmission of rotavirus-a. | we report here whole genome analysis of a porcine rotavirus-a (rva) strain rva/pig-wt/kna/et8b/2015/g5p[13] detected in a diarrheic piglet, and nearly whole genome (except for vp4 gene) analysis of a simian rva strain rva/simian-wt/kna/08979/2015/g5p[x] detected in a non-diarrheic african green monkey (agm) on the island of st. kitts, caribbean region. strain et8b exhibited a g5-p[13]-i5-r1-c1-m1-a8-n1-t7-e1-h1 genotype constellation that was identical to those of brazilian porcine rva g5p[13] s ... | 2017 | 28039076 |
| antisense oligonucleotides targeting raf-1 block japanese encephalitis virus in vitro and in vivo. | japanese encephalitis virus (jev) infections represent a major health concern in southeast asia since no effective treatments are available. recently, several reports have demonstrated that inhibition of certain host cell proteins prevents viral infection. raf-1 kinase is a central component of many signaling pathways involved in normal cell growth and oncogenic transformation, and ras/raf/erk signaling activation has been observed during viral infections (including jev infection). in this study ... | 2017 | 28051352 |
| detection of picobirnaviruses in vervet monkeys (chlorocebus sabaeus): molecular characterization of complete genomic segment-2. | during 2014-2015, 270 fecal samples were collected from non-diarrheic, captive and wild african green monkeys (agms) on the island of st. kitts, caribbean region. by rna-page, picobirnaviruses (pbvs) were detected in sixteen captive agms. by rt-pcr and sequencing of partial gene segment-2, pbvs in 15 of these 16 samples were assigned to genogroup-i. the full-length nucleotide (nt) sequence of gene segment-2 of one of the genogroup-i pbv strains, strain pbv/african green monkey/kna/016593/2015, w ... | 2017 | 28057480 |
| size-dependent cellular uptake mechanism and cytotoxicity toward calcium oxalate on vero cells. | urinary crystals with various sizes are present in healthy individuals and patients with kidney stone; however, the cellular uptake mechanism of calcium oxalate of various sizes has not been elucidated. this study aims to compare the internalization of nano-/micron-sized (50 nm, 100 nm, and 1 μm) calcium oxalate monohydrate (com) and dihydrate (cod) crystals in african green monkey renal epithelial (vero) cells. the internalization and adhesion of com and cod crystals to vero cells were enhanced ... | 2017 | 28150811 |
| characterization of a thienylcarboxamide derivative that inhibits the transactivation functions of cytomegalovirus ie2 and varicella zoster virus ie62. | previously we established reporter cell lines for human cytomegalovirus (hcmv) and varicella zoster virus (vzv) and identified several antiviral compounds against these viruses using the reporter cells. in this study, we found that one of the identified anti-hcmv compounds, a thienylcarboxamide derivative (coded as 133g4), was effective against not only hcmv but also vzv. the following findings indicate that 133g4 inhibits the activation of early gene promoters by hcmv ie2 and vzv ie62: i) 133g4 ... | 2017 | 28161581 |
| the demographic and adaptive history of the african green monkey. | relatively little is known about the evolutionary history of the african green monkey (genus chlorocebus) due to the lack of sampled polymorphism data from wild populations. yet, this characterization of genetic diversity is not only critical for a better understanding of their own history, but also for human biomedical research given that they are one of the most widely used primate models. here, i analyze the demographic and selective history of the african green monkey, utilizing one of the m ... | 2017 | 28199709 |
| γ-butenolide and furanone derivatives from the soil-derived fungus aspergillus sclerotiorum psu-rspg178. | chromatographic separation of the broth extract of the soil-derived fungus aspergillus sclerotiorum psu-rspg178 resulted in isolation of four γ-butenolide-furanone dimers, aspersclerotiorones a-d, a furanone derivative, aspersclerotiorone e, and two γ-butenolide derivatives, aspersclerotiorones f and g, together with six known compounds, penicillic acid, dihydropenicillic acid, 5,6-dihydro-6-hydroxypenicillic acid, 6-methoxy-5,6-dihydropenicillic acid, coculnol and (4r,5r)-4,5-dihydroxy-3-methox ... | 2017 | 28228227 |
| regulators of mitochondrial quality control differ in subcutaneous fat of metabolically healthy and unhealthy obese monkeys. | obesity exists with and without accompanying cardiometabolic disease, termed metabolically unhealthy obesity (muo) and healthy obesity (mho), respectively. underlying differences in the ability of subcutaneous (sq) fat to respond to nutrient excess are emerging as a key pathway. this study aimed to document the first spontaneous animal model of mho and muo and differences in sq adipose tissue. | 2017 | 28236433 |
| attenuated human parainfluenza virus type 1 expressing ebola virus glycoprotein gp administered intranasally is immunogenic in african green monkeys. | the recent 2014-2016 ebola virus (ebov) outbreak prompted increased efforts to develop vaccines against ebov disease. we describe the development and preclinical evaluation of an attenuated recombinant human parainfluenza virus type 1 (rhpiv1) expressing the membrane-anchored form of ebov glycoprotein gp, as an intranasal (in) ebov vaccine. gp was codon optimized and expressed either as a full-length protein or an engineered chimeric form in which its transmembrane and cytoplasmic tail (tmct) do ... | 2017 | 28250127 |
| comparison of saliva collection methods for the determination of salivary cortisol levels in rhesus macaques (macaca mulatta), cynomolgus macaques (macaca fascicularis), and african green monkeys (chlorocebus aethiops). | the ability to quickly and accurately determine cortisol as a biomarker for stress is a valuable tool in assessing thewellbeing of nhp. in this study, 2 methods of collecting saliva (a commercial collection device and passive drool) and the resulting free salivary cortisol levels were compared with total serum cortisol concentration in rhesus macaques (macaca mulatta),cynomolgus macaques (macaca fascicularis) and african green monkeys (chlorocebus aethiops) at 2 collection time points.serum and ... | 2017 | 28256186 |
| nanoethosomal formulation of gammaoryzanol for skin-aging protection and wrinkle improvement: a histopathological study. | free radical scavengers and antioxidants, with the main focus on enhanced targeting to the skin layers, can provide protection against skin ageing. | 2017 | 28277843 |
| seroprevalence of zika virus in wild african green monkeys and baboons. | zika virus (zikv) has recently spread through the americas and has been associated with a range of health effects, including birth defects in children born to women infected during pregnancy. although the natural reservoir of zikv remains poorly defined, the virus was first identified in a captive "sentinel" macaque monkey in africa in 1947. however, the virus has not been reported in humans or nonhuman primates (nhps) in africa outside gabon in over a decade. here, we examine zikv infection in ... | 2017 | 28289727 |
| a single dose recombinant piv5 expressing respiratory syncytial virus f and g proteins protected cotton rats and african green monkeys from rsv challenge. | human respiratory syncytial virus (rsv) is a common cause of severe respiratory disease among infants, immunocompromised individuals and the elderly. no licensed vaccine is currently available. in this study, we evaluated two parainfluenza virus 5 (piv5)-vectored vaccines expressing rsv f (piv5/f) or g (piv5/g) proteins in the cotton rat and african green monkey models for their replication, immunogenicity and efficacy of protection against rsv challenge. following a single intranasal inoculatio ... | 2017 | 28298602 |
| comparison of saliva collection methods for the determination of salivary cortisol levels in rhesus macaques (macaca mulatta), cynomolgus macaques (macaca fascicularis), and african green monkeys (chlorocebus aethiops). | the ability to quickly and accurately determine cortisol as a biomarker for stress is a valuable tool in assessing the wellbeing of nhp. in this study, 2 methods of collecting saliva (a commercial collection device and passive drool) and the resulting free salivary cortisol levels were compared with total serum cortisol concentration in rhesus macaques (macaca mulatta), cynomolgus macaques (macaca fascicularis) and african green monkeys (chlorocebus aethiops) at 2 collection time points. serum a ... | 2017 | 28315649 |
| convergent balancing selection on the mu-opioid receptor in primates. | the mu opioid receptor is involved in many natural processes including stress response, pleasure, and pain. mutations in the gene also have been associated with opiate and alcohol addictions as well as with responsivity to medication targeting these disorders. two common and mutually exclusive polymorphisms have been identified in humans, a118g (n40d), found commonly in non-african populations, and c17t (v6a), found almost exclusively in african populations. while a118g has been studied extensiv ... | 2017 | 28333316 |
| biofilm formation of hypermucoviscous and non-hypermucoviscous klebsiella pneumoniae recovered from clinically affected african green monkey (chlorocebus aethiops sabaeus). | in recent years, an emergent klebsiella pneumoniae hypermucoviscous (hmv) phenotype has been associated with increased invasiveness and pathogenicity in primates. the hmv phenotype is characterized by different capsular serotypes, associated with several genes including the rmpa (regulator of mucoid phenotype) and maga (mucoviscosity-associated) genes. in african green monkeys (agm) (chlorocebus aethiops sabaeus) serotypes k1 and k5 have been implicated in fatal multisystemic abscesses. in order ... | 2017 | 28366827 |
| sodium alginate and gum acacia hydrogels of zinc oxide nanoparticles reduce hemolytic and oxidative stress inflicted by zinc oxide nanoparticles on mammalian cells. | zinc oxide nanoparticles are important nanomaterials currently under research due to their applicability in nanomedicine. toxicity of zno nps has been extensively studied and has been shown to affect various cell types and animal systems. in this study, we investigated hemolytic potential and oxidative stress inflicted by zno nps and zno nps-loaded-sodium alginate-gum acacia hydrogels on horse erythrocytes and african green monkey kidney (vero) cells. our study provides a better understanding of ... | 2017 | 28373047 |
| loss in lung volume and changes in the immune response demonstrate disease progression in african green monkeys infected by small-particle aerosol and intratracheal exposure to nipah virus. | nipah virus (niv) is a paramyxovirus (genus henipavirus) that emerged in the late 1990s in malaysia and has since been identified as the cause of sporadic outbreaks of severe febrile disease in bangladesh and india. niv infection is frequently associated with severe respiratory or neurological disease in infected humans with transmission to humans through inhalation, contact or consumption of niv contaminated foods. in the work presented here, the development of disease was investigated in the a ... | 2017 | 28388650 |
| distribution of interstitial telomeric sequences in primates and the pygmy tree shrew (scandentia). | it has been hypothesized that interstitial telomeric sequences (itss), i.e., repeated telomeric dna sequences found at intrachromosomal sites in many vertebrates, could be correlated to chromosomal rearrangements and plasticity. to test this hypothesis, we hybridized a telomeric pna probe through fish on representative species of 2 primate infraorders, strepsirrhini (lemur catta, otolemur garnettii, nycticebus coucang) and catarrhini (erythrocebus patas, cercopithecus petaurista, chlorocebus aet ... | 2017 | 28423373 |
| effects of naturally occurring charged mutations on the structure, stability, and binding of the pin1 ww domain. | pin1 is a peptidyl-prolyl cis-trans isomerase, whose ww domain specifically recognizes the pser/thr-pro motif. pin1 is involved in multiple phosphorylation events that regulate the activities of various substrates, and pin1 deregulation has been reported in various diseases, including cancer and alzheimer's disease. the ww domain of pin1 has been used as a small model protein to investigate the folding mechanisms of the β-sheet structure by studying the effect of mutations or its naturally occur ... | 2017 | 28431929 |
| assessment of potential mirna biomarkers of vero-cell tumorigenicity in a new line (agmk1-9t7) of african green monkey kidney cells. | patterns of microrna expression appear to delineate the process of spontaneous neoplastic development-transformation (spndt) occurring in the african green monkey kidney (agmk) vero cell line (teferedegne et al., 2010). analysis of microarray data identified 6 micrornas whose high-level of expression peaked when the world health organization 10-87 vero cells became tumorigenic at passage (p) 190. six mirnas were identified as potential biomarkers for the expression of the vero-cell tumorigenic p ... | 2017 | 28434690 |
| cytotoxic t cell functions accumulate when cd4 is downregulated by cd4(+) t cells in african green monkeys. | african green monkeys (agms) are a natural host of siv that do not develop simian aids. adult agms naturally have low numbers of cd4(+) t cells and a large population of mhc class ii-restricted cd8αα t cells that are generated through cd4 downregulation in cd4(+) t cells. in this article, we study the functional profiles and siv infection status in vivo of cd4(+) t cells, cd8αα t cells, and cd8αβ t cells in lymph nodes, peripheral blood, and bronchoalveolar lavage fluid of agms and rhesus macaqu ... | 2017 | 28438898 |
| vaccine cigb 247 is potentially safe for use as a novel therapeutic vaccine against cancer in chlorocebus aethiops monkeys. | cigb 247 is a novel cancer therapeutic vaccine based on human vascular endothelial growth factor (vegf) variant molecule as antigen, in combination with a bacterial adjuvant. this vaccine candidate has previously demonstrated efficacy and safety in mice, rats, rabbits and non-human primates. in the present study we evaluated the effects on the clinical, hematological and biochemical parameters of cigb 247 vaccine in chlorocebus aethiops monkeys. three groups of monkeys were immunized with three ... | 2017 | 28463787 |
| characterization of porcine epidemic diarrhea virus infectivity in human embryonic kidney cells. | porcine epidemic diarrhea virus (pedv), a causative agent of porcine epidemic diarrhea, causes economic loss in the global swine industry. vero cell, an african green monkey kidney cell line, has been commonly used to isolate and propagate pedv. however, since the production of interferon in these cells is defective, vero cells are not the ideal cell type to study the molecular mechanisms of pedv infection and the host antiviral innate immune response. in this study, we observed that human embry ... | 2017 | 28470417 |
| radiosynthesis and in vivo evaluation of [(11)c]a1070722, a high affinity gsk-3 pet tracer in primate brain. | dysfunction of glycogen synthase kinase 3 (gsk-3) is implicated in the etiology of alzheimer's disease, parkinson's disease, diabetes, pain, and cancer. a radiotracer for functional positron emission tomography (pet) imaging could be used to study the kinase in brain disorders and to facilitate the development of small molecule inhibitors of gsk-3 for treatment. at present, there is no target-specific or validated pet tracer available for the in vivo monitoring of gsk-3. we radiolabeled the smal ... | 2017 | 28485573 |
| vacuolation activity and intracellular trafficking of artb, the binding subunit of an ab5 toxin produced by salmonella enterica serovar typhi. | various salmonella enterica serovars, including s. enterica serovar typhi, encode an ab5 toxin (artab), the a subunit of which is an adp-ribosyltransferase related to the s1 subunit of pertussis toxin. however, although the a subunit is able to catalyze adp-ribosylation of host g proteins, a cytotoxic phenotype has yet to be identified for the holotoxin. here we show that its b subunit pentamer (artb) binds to receptors on the surface of vero (african green monkey kidney) cell, cho (chinese hams ... | 2017 | 28533468 |
| hematologic and biochemical ris for an aged population of captive african green monkeys (chlorocebus aethiops sabaeus). | established ris for geriatric african green monkeys (chlorocebus aethiops sabaeus) are critical for clinical differentiation of normal aging from disease-related changes in this population. | 2017 | 28543372 |
| optimized method for isolation of immature intracytoplasmic retroviral particles from mammalian cells. | to biochemically and structurally characterize viral intracytoplasmic particles (icaps), a sample of high purity and homogeneity is usually required. production of icaps in the system closely related to their natural host cells is crucial for the analysis of host-cell binding proteins involved in icaps assembly, transport and budding. however, this approach is often hampered by problems with low yield of the icaps due to either low expression or fast release from the host cell. another obstacle ... | 2017 | 28619602 |
| time-dependent subcellular structure injuries induced by nano-/micron-sized calcium oxalate monohydrate and dihydrate crystals. | comparative studies were conducted to investigate the time effect of cell injury induced by nano-sized (50nm) and micron-sized (10μm) calcium oxalate monohydrate (com) and dihydrate (cod) crystals in african green monkey renal epithelial (vero) cells. the effects of nano-/micron-sized com and cod exposure on vero cells were investigated by detecting the cell viability, cell morphology, ldh release, reactive oxygen species, mitochondrial membrane potential, cell cycle, and cell apoptosis, as well ... | 2017 | 28629039 |
| milk oligosaccharides inhibit human rotavirus infectivity in ma104 cells. | background: oligosaccharides in milk act as soluble decoy receptors and prevent pathogen adhesion to the infant gut. milk oligosaccharides reduce infectivity of a porcine rotavirus strain; however, the effects on human rotaviruses are less well understood.objective: in this study, we determined the effect of specific and abundant milk oligosaccharides on the infectivity of 2 globally dominant human rotavirus strains.methods: four milk oligosaccharides-2'-fucosyllactose (2'fl), 3'-sialyllactose ( ... | 2017 | 28637685 |
| renal vascular and glomerular pathologies associated with spontaneous hypertension in the nonhuman primate chlorocebus aethiops sabaeus. | hypertension is a complex, multifactorial disease affecting an estimated 78 million adults in the united states. despite scientific gains, the etiology of human essential hypertension is unknown and current experimental models do not recapitulate all the behavioral and physiological characteristics of the pathology. researchers should assess the translational capacity of these models and look to other animal models for the discovery of new therapies. chlorocebus aethiops sabaeus, the african gre ... | 2017 | 28659284 |
| modern-day siv viral diversity generated by extensive recombination and cross-species transmission. | cross-species transmission (cst) has led to many devastating epidemics, but is still a poorly understood phenomenon. hiv-1 and hiv-2 (human immunodeficiency virus 1 and 2), which have collectively caused over 35 million deaths, are the result of multiple csts from chimpanzees, gorillas, and sooty mangabeys. while the immediate history of hiv is known, there are over 45 lentiviruses that infect specific species of primates, and patterns of host switching are not well characterized. we thus took a ... | 2017 | 28672035 |
| comparison of gastrointestinal parasite communities in vervet monkeys. | 2017 | 28685946 | |
| in vitro and in vivo anticancer studies of 2'-hydroxy chalcone derivatives exhibit apoptosis in colon cancer cells by hdac inhibition and cell cycle arrest. | considering the therapeutic values of bioflavonoids in colon cancer treatment, six 2'-hydroxy chalcones (c1-c6) were synthesized, characterized and screened for in vitro cytotoxicity on human colon carcinoma (hct116) and african green monkey kidney epithelial cells (vero). only c5 showed selective cytotoxicity against hct116 cells. other potent cytotoxic compounds were c1, c2 and c3. further screening included enzyme inhibition studies on histone deacetylase (hdac) enzyme where c1 showed lowest ... | 2017 | 28694750 |
| smart release nano-formulation of cytochrome c and hyaluronic acid induces apoptosis in cancer cells. | herein we tested a nanosized cancer-cell targeted delivery system based on cytochrome c (cyt c) and hyaluronic acid. cyt c was chosen since it is a per se non-toxic protein but causes apoptosis when delivered to the cytoplasm of target cells. hyaluronic acid was employed to create the nanosized delivery system with passive targeting capability in order to exploit the enhanced permeation and retention (epr) effect and active targeting capability of hyaluronic acid. in addition, our goal was to in ... | 2017 | 28706754 |
| the genetic stability of piv5-vectored rsv vaccine candidates after in vitro and in vivo passage. | human respiratory syncytial virus (rsv) is the leading etiologic agent of lower respiratory tract infections in children, but no licensed vaccine exists. previously, our laboratory developed two parainfluenza virus 5 (piv5)-based rsv vaccine candidates that protect mice against rsv challenge. piv5 was engineered to express either the rsv fusion protein (f) or the rsv major attachment glycoprotein (g) between the hn and l genes of the piv5 genome [piv5-rsv-f (hn-l) or piv5-rsv-g (hn-l)]. to inves ... | 2017 | 28747497 |
| identification and characterization of the tyrosinase gene (tyr) and its transcript variants (tyr_1 and tyr_2) in the crab-eating macaque (macaca fascicularis). | tyrosinase is a copper-containing enzyme that regulates melanin biosynthesis and is encoded by the tyrosinase (tyr) gene. previous studies demonstrated that mutations in tyr could lead to oculocutaneous albinism type 1 (oca1) owing to the failure of melanin formation. although a previous study found that albinism in the rhesus monkey was derived from a mutation in tyr, the identification and characterization of this gene in non-human primates has not been achieved thus far. thus, using the rapid ... | 2017 | 28756020 |
| filovirus research: how it began. | the first reported filovirus outbreak occurred in august 1967, when laboratory workers in marburg and frankfurt, germany, and belgrade, yugoslavia (now serbia) became infected with an unknown highly pathogenic agent. the disease was characterized by high fever, malaise, rash, hemorrhagic and tetanic manifestations, and high lethality, amounting to 25%. the disease was introduced to europe by grivets (chlorocebus aethiops), which were used for biomedical research and vaccine production. the causa ... | 2017 | 28766193 |
| cyclophilin a potentiates trim5α inhibition of hiv-1 nuclear import without promoting trim5α binding to the viral capsid. | the host immunophilin cyclophilin a (cypa) binds to the capsid protein (ca) of hiv-1 and regulates its infectivity. depending on the target cell type, cypa can either promote or inhibit hiv-1 infection. the ability of cypa to promote hiv-1 infection has been extensively studied and linked to several steps in early replication including uncoating, reverse transcription and nuclear import. by contrast, the mechanism by which cypa inhibits infection is less well understood. we investigated the mech ... | 2017 | 28767697 |
| genomic tools for the use of nonhuman primates in translational research. | nonhuman primates (nhps) are important preclinical models for understanding the etiology of human diseases and for developing therapies and vaccines to cure or eliminate disease. most human diseases have genetic components. therefore, to be of maximal utility, the nhp species used for translational science should be as well characterized in regard to their genome and transcriptome as possible. this article reviews the current status of genomic information for the five nhp species used most often ... | 2017 | 28838069 |
| standardization of dna residual quantification method of vero cell rabies vaccine for human use. | normalize the quantification of residual dna from vero cells in the rabies vaccine for use in human vahv i, by quantitative pcr in real time and the design of primers that amplified, highly repetitive sequences of cercopithecus aethiops and a constitutive gene according to sequences reported in the genbank and quantifying the residual dna in the vaccine vahv i in three consecutive batches according to the standard set by the world health organization. | 2017 | 28839473 |
| inflammatory monocytes expressing tissue factor drive siv and hiv coagulopathy. | in hiv infection, persistent inflammation despite effective antiretroviral therapy is linked to increased risk of noninfectious chronic complications such as cardiovascular and thromboembolic disease. a better understanding of inflammatory and coagulation pathways in hiv infection is needed to optimize clinical care. markers of monocyte activation and coagulation independently predict morbidity and mortality associated with non-aids events. we identified a specific subset of monocytes that expre ... | 2017 | 28855397 |
| structural investigation of c6/36 and vero cell cultures infected with a brazilian zika virus. | zika virus (zikv) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand rna enclosed in a capsid and surrounded by a membrane. studies on the replication dynamics of zikv are scarce, which limits the development of antiviral agents and vaccines directed against zikv. in this study, aedes albopictus mosquito lineage cells (c6/36 cells) and african green monkey kidney epithelial cells (vero cells) were inoculated with a zikv sample isolated from a b ... | 2017 | 28898286 |
| pathogenic correlates of simian immunodeficiency virus-associated b cell dysfunction. | we compared and contrasted pathogenic (in pig-tailed macaques [ptms]) and nonpathogenic (in african green monkeys [agms]) sivsab infections to assess the significance of the b cell dysfunction observed in simian (siv) and human immunodeficiency virus (hiv) infections. we report that the loss of b cells is specifically associated with the pathogenic siv infection, while in the natural hosts, in which siv is nonpathogenic, b cells rapidly increase in both lymph nodes (lns) and intestine. siv-assoc ... | 2017 | 28931679 |
| synthesis, characterization and biological applications of mycosynthesized silver nanoparticles. | silver nanoparticles (agnps) have been known for their inhibitory and bactericidal effects. in the present study, less toxic agnps using cunninghamella echinulata is reported for the first time. the obtained agnps were characterized using uv-visible spectrophotometer, xrd, ft-ir, fe-sem with edax and hr-tem. agnps showed the maximum absorbance at 420-430 nm. the transmission electron micrograph revealed the formation of considerably uniform-sized agnps with an average size of 20-50 nm. the reduc ... | 2017 | 28955630 |
| in vitro cytotoxicity of zinc oxide, iron oxide and copper nanopowders prepared by green synthesis. | in vitro cytotoxic effects of zno, feo and cu metallic nanopowders (nps) on vero (african green monkey kidney cell line), pk 15 (pig kidney cell line) and madin darby bovine kidney (mdbk) cell lines were investigated at different time intervals (24 and 48 h). mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine the cytotoxic effects of green synthesized (plant based) nanopowders. the comparative effects of exposure period and concentration of nanopowders ... | 2017 | 28959669 |
| natural killer cells migrate into and control simian immunodeficiency virus replication in lymph node follicles in african green monkeys. | natural killer (nk) cells play an essential role in antiviral immunity, but knowledge of their function in secondary lymphoid organs is incomplete. lymph node follicles constitute a major viral reservoir during infections with hiv-1 and simian immunodeficiency virus of macaques (sivmac). in contrast, during nonpathogenic infection with siv from african green monkeys (sivagm), follicles remain generally virus free. we show that nk cells in secondary lymphoid organs from chronically sivagm-infecte ... | 2017 | 29035370 |
| direct estimate of the spontaneous germ line mutation rate in african green monkeys. | here, i provide the first direct estimate of the spontaneous mutation rate in an old world monkey, using a seven individual, three-generation pedigree of african green monkeys. eight de novo mutations were identified within ∼1.5 gbp of accessible genome, corresponding to an estimated point mutation rate of 0.94 × 10-8per site per generation, suggesting an effective population size of ∼12000 for the species. this estimation represents a significant improvement in our knowledge of the population g ... | 2017 | 29068052 |
| peripheral blood biomarkers of disease outcome in a monkey model of rift valley fever encephalitis. | rift valley fever (rvf) is an emerging arboviral disease of livestock and humans. although the disease is caused by a mosquito-borne virus, humans are infected through contact with, or inhalation of, virus-laden particles from contaminated animal carcasses. some individuals infected with rvf virus (rvfv) develop meningoencephalitis, resulting in morbidity and mortality. little is known about the pathogenic mechanisms that lead to neurologic sequelae, and thus, animal models that represent human ... | 2018 | 29118127 |
| respiratory syncytial virus elicits enriched cd8+ t lymphocyte responses in lung compared with blood in african green monkeys. | respiratory syncytial virus (rsv) is a leading cause of serious lower respiratory tract disease in young children and older adults throughout the world. prevention of severe rsv disease through active immunization is optimal but no rsv vaccine has been licensed so far. immune mechanisms of protection against rsv infection in humans have not been fully established, thus a comprehensive characterization of virus-specific immune responses in a relevant animal model will be beneficial in defining co ... | 2017 | 29121080 |
| siv coreceptor specificity in natural and non-natural host infection: implications for cell targeting and differential outcomes from infection. | pathogenic hiv-1 infection of humans and sivmac infection of macaques are the result of zoonotic transfer of primate immunodeficiency viruses from their natural hosts into non-natural host species. natural host infections do not result in pathogenesis despite high levels of virus replication, and evidence suggests that differences in anatomical location and specific subsets of cd4+ t cells infected may underlie distinct outcomes from infection. the coreceptor ccr5 has long been considered the so ... | 2017 | 29173179 |
| bobcats (lynx rufus) are natural definitive host of besnoitia darlingi. | bovine besnoitiosis, caused by besnoitia besnoiti, is an economically important disease of cattle in many countries but its transmission remains a mystery. wild felids are suspected to be its definitive hosts. the domestic cat (felis catus) is known experimental definitive host for besnoitia species of rodents. here, we report for besnoitia darlingi the first identification of a natural definitive host, the bobcat (lynx rufus). oocysts resembling toxoplasma gondii (unsporulated; 10.9±0.8×12.1±0. ... | 2017 | 29173547 |
| antiviral activity and metal ion-binding properties of some 2-hydroxy-3-methoxyphenyl acylhydrazones. | here we report on the results obtained from an antiviral screening, including herpes simplex virus, vaccinia virus, vesicular stomatitis virus, coxsackie b4 virus or respiratory syncytial virus, parainfluenza-3 virus, reovirus-1 and punta toro virus, of three 2-hydroxy-3-methoxyphenyl acylhydrazone compounds in three cell lines (i.e. human embryonic lung fibroblast cells, human cervix carcinoma cells, and african green monkey kidney cells). interesting antiviral ec50 values are obtained against ... | 2018 | 29209895 |
| obesity and obesogenic growth are both highly heritable and modified by diet in a nonhuman primate model, the african green monkey (chlorocebus aethiops sabaeus). | in humans, the ontogeny of obesity throughout the life course and the genetics underlying it has been historically difficult to study. we compared, in a non-human primate model, the lifelong growth trajectories of obese and non-obese adults to assess the heritability of and map potential genomic regions implicated in growth and obesity. | 2017 | 29211707 |
| pulmonary edema following central nervous system lesions induced by a non- mouse-adapted ev71 strain in neonatal balb/c mice. | enterovirus (ev) infection has been a serious health issue in asia-pacific region. it has been indicated that the occurrence of fatal hand foot and mouth disease (hfmd) cases following ev71 infection is mainly attributed to pulmonary edema. however, the development of pulmonary disorders after ev71 infection remains largely unknown. to establish an ev71-infected animal model and further explore the underlying association of central nervous system (cns) invasion with pulmonary edema, we isolated ... | 2017 | 29282065 |
| novel endogenous simian retroviral integrations in vero cells: implications for quality control of a human vaccine cell substrate. | african green monkey (agm)-derived vero cells have been utilized to produce various human vaccines. the vero cell genome harbors a variety of simian endogenous type d retrovirus (serv) sequences. in this study, a transcriptome analysis showed that dna hypomethylation released the epigenetic repression of servs in vero cells. moreover, comparative genomic analysis of three vero cell sublines and an agm reference revealed that the genomes of the sublines have ~80 serv integrations. among them, ~60 ... | 2018 | 29330501 |
| complete genome sequence of the african green monkey simian foamy virus serotype 3 strain fv2014 (sfvcae_fv2014). | the full-length sequence of simian foamy virus serotype 3 (sfv-3) strain fv2014, an african green monkey (chlorocebus aethiops) isolate, was obtained using high-throughput sequencing. sfvcae_fv2014 consisted of 13,127 bp and had a genomic organization similar to those of other sfvs but was distinct from sfv strain lk3, isolated from the same monkey species. | 2018 | 29348335 |
| aging african green monkeys manifest transcriptional, pathological, and cognitive hallmarks of human alzheimer's disease. | while many preclinical models of alzheimer's disease (ad) have been reported, none fully recapitulate the disease. in an effort to identify an appropriate preclinical disease model, we characterized age-related changes in 2 higher order species, the african green monkey (agm) and the rhesus macaque. gene expression profiles in the dorsolateral prefrontal cortex and the visual cortex showed age-related changes in agms that are strikingly reminiscent of ad, whereas aged rhesus were most similar to ... | 2017 | 29353102 |
| highly sensitive bioluminescent probe for thiol detection in living cells. | the sensitive detection of thiols including glutathione and cysteine is desirable owing to their roles as indispensable biomolecules in maintaining intracellular biological redox homeostasis. herein, we report the design and synthesis of seluc-1 (sulfinate ester luciferin), a chemoselective probe exhibiting a ratiometric and turn-on response towards thiols selectively in fluorescence and bioluminescence, respectively. the probe, which was designed based on the "caged" luciferin strategy, display ... | 2018 | 29359483 |
| renal epithelial cell injury induced by calcium oxalate monohydrate depends on their structural features: size, surface, and crystalline structure. | urinary crystals in normal and kidney stone patients often differ in crystal sizes and surface structures, but the effects of different crystal properties on renal tubular epithelial cells remain unclear. this study aimed to compare the cytotoxicity of micron/nano-calcium oxalate monohydrate (com) crystals with sizes of 50 nm, 200 nm, 1 μm, 3 μm, and 10 μm to african green monkey renal epithelial (vero) cells, to reveal the effect of crystal size and surface structure on cell injury, and to inve ... | 2016 | 29364612 |
| autosomal recessive congenital cataract in captive-bred vervet monkeys (chlorocebus aethiops). | the aim of the study was to evaluate the genetic predisposition of congenital cataract in a colony of captive-bred vervet monkeys. | 2018 | 29377145 |
| the choice of linker for conjugating r848 to inactivated influenza virus determines the stimulatory capacity for innate immune cells. | inactivated influenza vaccines are not approved for use in infants less than 6 months of age due to poor immunogenicity in that population. while the live attenuated influenza vaccine has the potential to be more immunogenic, it is not an option for infants and other vulnerable populations, including the elderly and immunocompromised individuals due to safety concerns. in an effort to improve the immunogenicity of the inactivated vaccine for use in vulnerable populations, we have used an approac ... | 2018 | 29398273 |
| havcr1 (cd365) and its mouse ortholog are functional hepatitis a virus (hav) cellular receptors that mediate hav infection. | the hepatitis a virus (hav) cellular receptor 1 (havcr1), classified as cd365, was initially discovered as a hav cellular receptor using an expression cloning strategy. due to the lack of hav receptor-negative replication-competent cells, it was not possible to fully prove that havcr1 was a functional hav receptor. however, biochemistry, classical virology, and epidemiology studies further supported the functional role of havcr1 as a hav receptor. here, we show that an anti-havcr1 monoclonal ant ... | 2018 | 29437974 |
| intranasal live influenza vaccine priming elicits localized b cell responses in mediastinal lymph nodes. | pandemic live attenuated influenza vaccines (plaiv) prime subjects for a robust neutralizing antibody response upon subsequent administration of inactivated subunit vaccine (pisv). however, a difference was not detected in h5-specific memory b cells in the peripheral blood between plaiv-primed and unprimed subjects prior to pisv boost. to investigate the mechanism underlying plaiv priming, we vaccinated groups of 12 african green monkeys (agms) with h5n1 pisv or plaiv alone or h5n1 plaiv followe ... | 2018 | 29444938 |
| schistosoma mansoni and other intestinal parasitic infections in schoolchildren and vervet monkeys in lake ziway area, ethiopia. | to assess schistosoma mansoni and other intestinal parasitic infections in schoolchildren and vervet monkeys (chlorocebus aethiops) in bochessa village, ziway, ethiopia. | 2018 | 29463304 |
| neurodegenerative disease biomarkers aβ1-40, aβ1-42, tau, and p-tau181 in the vervet monkey cerebrospinal fluid: relation to normal aging, genetic influences, and cerebral amyloid angiopathy. | the caribbean vervet monkey (chlorocebus aethiops sabaeus) is a potentially valuable animal model of neurodegenerative disease. however, the trajectory of aging in vervets and its relationship to human disease is incompletely understood. | 2018 | 29484263 |
| predominant envelope variable loop 2-specific and gp120-specific antibody-dependent cellular cytotoxicity antibody responses in acutely siv-infected african green monkeys. | the initial envelope (env)-specific antibody response in acutely hiv-1-infected individuals and simian immunodeficiency virus (siv)-infected rhesus monkeys (rms) is dominated by non-neutralizing antibodies targeting env gp41. in contrast, natural primate siv hosts, such as african green monkeys (agms), develop a predominant env gp120-specific antibody response to siv infection. however, the fine-epitope specificity and function of siv env-specific plasma igg, and their potential role on autologo ... | 2018 | 29523166 |
| immunogenicity in african green monkeys of m protein mutant vesicular stomatitis virus vectors and contribution of vector-encoded flagellin. | recombinant vesicular stomatitis virus (vsv) is a promising platform for vaccine development. m51r vsv, an attenuated, m protein mutant strain, is an effective inducer of type i interferon and dendritic cell (dc) maturation, which are desirable properties to exploit for vaccine design. we have previously evaluated m51r vsv (m51r) and m51r vsv that produces flagellin (m51r-f) as vaccine vectors using murine models, and found that flagellin enhanced dc activation and vsv-specific antibody producti ... | 2018 | 29562688 |
| interaction of porcine reproductive and respiratory syndrome virus major envelope proteins gp5 and m with the cellular protein snapin. | porcine reproductive and respiratory syndrome (prrs) is characterized by abortions in pregnant sows and respiratory disease, particularly in young pigs. the causative agent is porcine reproductive and respiratory syndrome virus (prrsv), a member of the arterivirus family. gp5 and m are the major envelope proteins encoded by prrsv. to further characterize these two viral proteins, a yeast two-hybrid approach was utilized to identify interacting partners of prrsv gp5 and m proteins. | 2018 | 29577951 |
| identity construction and symbolic association in farmer-vervet monkey (chlorocebus aethiops sabaeus) interconnections in st. kitts. | primates occupy a liminal space between humans and animals. on the caribbean island of st. kitts, translocated vervet monkeys (chlorocebus aethiops sabaeus) are respected creatures, yet they cause problems. vervets regularly consume crops on farms and are considered "pests"; still, kittitians express empathy and understanding for them based largely on the monkeys' display of human-like behaviours. using data from interviews with 64 kittitian farmers, we deconstruct the symbolism of the vervet mo ... | 2018 | 29631262 |
| species-specific host factors rather than virus-intrinsic virulence determine primate lentiviral pathogenicity. | hiv-1 causes chronic inflammation and aids in humans, whereas related simian immunodeficiency viruses (sivs) replicate efficiently in their natural hosts without causing disease. it is currently unknown to what extent virus-specific properties are responsible for these different clinical outcomes. here, we incorporate two putative hiv-1 virulence determinants, i.e., a vpu protein that antagonizes tetherin and blocks nf-κb activation and a nef protein that fails to suppress t cell activation via ... | 2018 | 29636452 |
| loss of cxcr6 coreceptor usage characterizes pathogenic lentiviruses. | pandemic hiv-1 originated from the cross-species transmission of sivcpz, which infects chimpanzees, while sivcpz itself emerged following the cross-species transmission and recombination of monkey sivs, with env contributed by the sivgsn/mus/mon lineage that infects greater spot-nosed, mustached and mona monkeys. sivcpz and hiv-1 are pathogenic in their respective hosts, while the phenotype of their sivgsn/mus/mon ancestors is unknown. however, two well-studied siv infected natural hosts, sooty ... | 2018 | 29659623 |
| successful application of human-based methyl capture sequencing for methylome analysis in non-human primate models. | the characterization of genomic or epigenomic variation in human and animal models could provide important insight into pathophysiological mechanisms of various diseases, and lead to new developments in disease diagnosis and clinical intervention. the african green monkey (agm; chlorocebus aethiops) and cynomolgus monkey (cm; macaca fascicularis) have long been considered important animal models in biomedical research. however, non-human primate-specific methods applicable to epigenomic analyses ... | 2018 | 29669513 |
| a comparison between the semen and sperm parameters from the captive-bred vervet monkey (chlorocebus aethiops) and rhesus monkey (macaca mulatta). | it is generally accepted that non-human primates (nhp) represent the model of choice for integrative studies of testicular function and endocrine control. however, there are many species-specific differences that necessitate identification prior to the selection of an appropriate model for these studies. in an nhp breeding facility, this opportunity of selection is usually presented during breeding periods when it is crucial to determine which individuals should be maintained as breeders. with r ... | 2018 | 29693257 |
| cathelicidin-derived antimicrobial peptides inhibit zika virus through direct inactivation and interferon pathway. | zika virus (zikv) is a neurotrophic flavivirus that is able to infect pregnant women and cause fetal brain abnormalities. although there is a significant effort in identifying anti-zikv strategies, currently no vaccines or specific therapies are available to treat zikv infection. antimicrobial peptides, which are potent host defense molecules in nearly all forms of life, have been found to be effective against several types of viruses such as hiv-1 and influenza a. however, they have not been te ... | 2018 | 29706959 |
| lymph node cellular and viral dynamics in natural hosts and impact for hiv cure strategies. | combined antiretroviral therapies (carts) efficiently control hiv replication leading to undetectable viremia and drastic increases in lifespan of people living with hiv. however, cart does not cure hiv infection as virus persists in cellular and anatomical reservoirs, from which the virus generally rebounds soon after cart cessation. one major anatomical reservoir are lymph node (ln) follicles, where hiv persists through replication in follicular helper t cells and is also trapped by follicular ... | 2018 | 29725327 |
| electrospun chitosan/polycaprolactone-hyaluronic acid bilayered scaffold for potential wound healing applications. | fabrication of mechanically stable, biocompatible bilayered polymeric scaffold consisting of chitosan(cs)/polycaprolactone(pcl) and hyaluronic acid(ha) using less toxic solvent system is presented in this study. electrospinning technique to make the scaffold was used followed by morphological, physiochemical and mechanical characterizations. average fiber diameter of cs/pcl-ha bilayered scaffold was found 362.2 ± 236 nm which is in the range of collagen fiber found in the extracellular matrices. ... | 2018 | 29777811 |
| signature microrna expression profile is associated with spontaneous hypertension in african green monkey. | chlorocebus aethiops sabaeus, the african green monkey (agm), has been proved to exhibit renal vascular remodeling and spontaneous hypertension. however, little is known about the roles of micrornas (mirnas) in this process.using small rna deep sequencing, we compared the plasma mirna expression patterns between hypertensive (ht) agms and normotensive (nt) agms. expression of mirnas (mir-122, mir-339, mir-296-5p) was validated independently in plasma samples from 10 ht agms and 10 nt agms (fold ... | 2019 | 29787292 |
| behavioral, pharmacokinetic, and cardiovascular evaluation of candidate oximes in african green monkeys. | oxime reactivators are critical antidotes after organophosphate pesticide or nerve agent poisoning, directly restoring the function of inhibited acetylcholinesterase. in the continuing search for more broad-spectrum acetylcholinesterase reactivators, this study evaluated one of the leading next-generation oxime reactivators: methoxime, (1,1'-trimethylene bis[4-(hydroxyimino)methyl]pyridinium dichloride (mmb-4). the pharmacokinetics of both salts of mmb-4 (dichloride [2cl] and dimethanesulphonate ... | 2019 | 29879849 |
| hiv-2/siv viral protein x counteracts hush repressor complex. | to evade host immune defences, human immunodeficiency viruses 1 and 2 (hiv-1 and hiv-2) have evolved auxiliary proteins that target cell restriction factors. viral protein x (vpx) from the hiv-2/sivsmm lineage enhances viral infection by antagonizing samhd1 (refs 1,2), but this antagonism is not sufficient to explain all vpx phenotypes. here, through a proteomic screen, we identified another vpx target-hush (tasor, mpp8 and periphilin)-a complex involved in position-effect variegation3. hush dow ... | 2018 | 29891865 |