Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [second report on intestinal parasites among the patients of seoul paik hospital (1984-1992)]. | the results of fecal examination for helminth eggs and protozoan cysts in seoul paik hospital during 1984-1992 are reported. fecal specimens of a total of 52,552 out- or inpatients were examined by formalin-ether sedimentation and/or direct smear method. the overall egg positive rate of helminths was 6.5% and the cyst positive rate of protozoa 2.5%. the egg positive rate (number of positive cases) for each species of helminth was; clonorchis sinensis 3.2% (1,667), trichuris trichiura 2.0% (1,089 ... | 1994 | 8167105 |
| [analysis of pathogenicity of entamoeba histolytica by polymerase chain reaction]. | dna from five isolates of entamoeba histolytica were examined for their pathogenicity by polymerase chain reaction. three isolates sh-3,sh-6,sh-8 were isolated from patients with acute amoebic dysentery, whereas sh-5 and sh-7 were isolated from asymptomatic cyst passers. gel electrophoresis of pcr products showed that primers p11, p12 for pathogenic strains could amplify genomic dna extracted from sh-8, and primers p13, p14 for nonpathogenic strains could amplify genomic dna extracted from sh-3, ... | 1993 | 8168237 |
| parasitic infection among children living in two orphanages in cairo. | children living in institutions as orphanages are more exposed to intestinal parasitism, since crowding and behavioral pattern contribute greatly to the spread of parasitic infection. the present study was conducted on 100 children living in ain-shams and el-mowassa orphanages, cairo. twenty children living under appropriate health conditions were studied as controls. ages of both groups ranged from 6-12 years. the results of this study revealed that 69/100 (69%) orphanage children were positive ... | 1994 | 8169435 |
| the prevalence of infection by intestinal parasites in an urban slum in são paulo, brazil. | sedimentation parasitological examinations were performed in stool specimens from a random household-based sample of 407 dwellers, all age groups being represented, in a slum of são paulo, south-eastern brazil. intestinal parasites were detected in 45.7% of the specimens. the most prevalent parasites in this population were ascaris lumbricoides (23.8%) and trichuris trichiura (17.2%); only 17 individuals (4.2%) harboured giardia duodenalis and 6 (1.5%) harboured entamoeba histolytica. taenia and ... | 1994 | 8170003 |
| a comparative study of different isolated fractions of entamoeba histolytica in amoebic liver abscess. | fifty six patients with amoebic liver ubscess were assessed for antibody production and lymphokine release using different antigenic fractions such as sephadex g-200 eluted fraction-i (f-i), shed of bile salt treated amoebae (sbsta), amoebic membrane glycoprotein (amg), amoebic rna and whole amoebic lysate (wal). antibody production in response to wal, f-i, sbsta, amg and rna was assessed by indirect haemagglutination assay. five and 53 fold increased titres of iha was observed with f-i and sbst ... | 1993 | 8171729 |
| cardiac tamponade secondary to intrapericardial rupture of a hepatic amoebic abscess. | we report the case of a patient who underwent an emergency subxiphoid pericardiectomy with evacuation of 1700 cc of anchovy sauce-like fluid. on pericardial inspection a fistulous communication was seen with a cavity in the left hepatic lobe. no loculi or adhesions were found in the pericardial sac and no further surgical procedure was advised. the hepatic cavity and pericardium were drained: bacteriological investigation of the fluid yielded entamoeba histolytica and chloroquine was administere ... | 1994 | 8172716 |
| ultrastructural arguments for cytoplasmic engulfment phenomenon in nucleus of entamoeba histolytica. | the electron optic study of a pathogenic e. histolytica strain revealed particular aspects concerning the shape, structure and distribution in the nuclear space of the intranuclear structures known as button-like bodies. these aspects are ultrastructural arguments useful in interpreting the intranuclear inclusions as sections through the cytoplasmic material sequestered in the nuclear space by karyophagocytosis. | 1993 | 8173125 |
| cloning and expression of the gene for an nadp(+)-dependent aldehyde dehydrogenase of entamoeba histolytica. | 1994 | 8183316 | |
| molecular cloning of an entamoeba histolytica gene encoding a putative mos family serine/threonine-kinase. | molecular cloning techniques were used to isolate an ameba gene encoding a putative 33-kda serine/thyronine-kinase. the open reading frame of this gene, called ehmfk1 for e. histolytica mos family kinase, shows a 24% positional identity with the human proto-oncogene mos, including 32 of 33 amino acids conserved in other serine/threonine-kinases. | 1994 | 8186259 |
| protection of gerbils from amebic liver abscess by immunization with a recombinant protein derived from the 170-kilodalton surface adhesin of entamoeba histolytica. | the protozoan parasite entamoeba histolytica causes extensive morbidity and mortality worldwide through intestinal infection and amebic liver abscess. here we show that vaccination of gerbils, a standard model for amebic liver abscess, with recombinant proteins derived from the 170-kda galactose-binding adhesin of e. histolytica and the serine-rich e. histolytica protein or a combination of the two recombinant antigens provides excellent protection against subsequent hepatic challenge with virul ... | 1994 | 8188384 |
| use of micro samples of finger prick blood dried on filter paper for a quick and simple dipstick dot-eia for diagnosis of amebic liver abscess (ala). | filter paper was used as a support to absorb micro samples of finger prick blood for detection of antibodies to entamoeba histolytica (causative organism of amebiasis) by a rapid dipstick dot-eia technique; 8 microliters of blood was sufficient to saturate discs (diameter 6 mm) of whatman paper no. 3. conditions of elution of blood from the discs were optimized and the best results were obtained when 0.4 ml of buffer was used for elution for 30 minutes at room temperature. the filter paper techn ... | 1994 | 8189328 |
| cloning and sequence determination of the gene coding for the pyruvate phosphate dikinase of entamoeba histolytica. | we isolated three overlapping clones from a dna genomic library of entamoeba histolytica strain hm1:imss, whose translated nucleotide (nt) sequence shows similarities of 51, 48 and 47% with the amino acid (aa) sequences reported for the pyruvate phosphate dikinases from bacteroides symbiosus, maize and flaveria trinervia, respectively. the reading frame determined codes for a protein of 886 aa. | 1994 | 8194759 |
| entamoeba histolytica: electron-dense granule secretion, collagenase activity and virulence are altered in the cytoskeleton mutant bg-3. | hm-1:imss, a pathogenic strain of entamoeba histolytica, and its mutant bg-3, identified by resistance to cytochalasin d, were tested for their capacity to: (i) secrete electron-dense granules; (ii) adhere and digest native type i collagen gels; and (iii) produce liver abscesses in new-born hamsters. the results demonstrate that the mutant has low adherence to collagen, low electron-dense granule secretion and collagenolytic activity, and low capacity to produce liver lesions in vivo, compared w ... | 1994 | 8196550 |
| amoebiasis: a rare cause of cardiac tamponade. | cardiac tamponade secondary to perforation of a hepatic amoebic abscess developed six years after the patient had visited an area where entamoeba histolytica is endemic. he was treated with metronidazole and imipenem, emergency percutaneous catheter drainage, and open surgical drainage. | 1994 | 8198889 |
| role of mucosal secretory immunity in the development of an amebiasis vaccine. | invasive colonic infection by the enteric protozoan entamoeba histolytica elicits a mucosal anti-amebic iga antibody response. the e. histolytica galactose-inhibitable adherence protein (giap) mediates parasite binding to colonic mucins and epithelial cells. anti-giap secretory iga antibodies are found during invasive amebiasis and can be elicited by immunization of rats with the native protein. entamoeba histolytica contains potent iga degradative activities due to its cysteine-specific proteas ... | 1994 | 8203722 |
| change of isoenzyme pattern during long-term polyxenic cultivation of entamoeba histolytica. | isoenzymes of phosphoglucomutase and hexokinase were repeatedly evaluated using starch gel electrophoresis in polyxenic cultures of entamoeba histolytica. in two out of 18 strains spontaneous changes of isoenzyme patterns were recorded. while originally they were categorized into virulent group of zymodemes, following isoenzyme analysis classified them as non-virulent. the relation between virulence and isoenzyme pattern is questionable. | 1993 | 8215112 |
| synthesis of some 4-oxo-delta 2-thiazolin-2-ylhydrazones as potential antiprotozoal agents. | several 4-oxo-delta 2-thiazolin-2-ylhydrazone derivatives 6 (6-1 - 6-19) derived from the cyclization reaction between the thiosemicarbazones 5 and homologous alpha-chloroacids were prepared. only compound 6-2 was found to be active against entamoeba histolytica and trichomonas vaginalis. | 1993 | 8216678 |
| the calcium binding protein of entamoeba histolytica: expression in escherichia coli and immunochemical characterization. | the gene encoding calcium binding protein (cabp) of entamoeba histolytica is single-copy and transcribes a single class of mrna. the coding region of the gene, amplified from a genomic clone of cabp by polymerase chain reaction, was cloned into the escherichia coli expression vector pet-3c. the expressed protein was 15 kd in size, which agreed with the predicted size from the open reading frame. the recombinant protein showed altered mobility in sodium dodecyl sulfate-polyacrylamide gel electrop ... | 1993 | 8220585 |
| purification of helicobacter pylori superoxide dismutase and cloning and sequencing of the gene. | the superoxide dismutase (sod) of helicobacter pylori, a pathogenic bacterium which colonizes the gastric mucosa, evoking a marked inflammatory response, was purified and characterized, and the n-terminal amino acid sequence was determined. the enzyme consists of two identical subunits each with an apparent molecular weight of 24,000. analysis of the primary structure and inhibition studies revealed that h. pylori possesses a typical procaryotic iron-containing enzyme. no other isoenzymes could ... | 1993 | 8225605 |
| influence of bacteria on electrophoretic proteinase patterns of entamoeba histolytica isolates. | gelatin sds- polyacrylamide gel electrophoresis was used to compare proteinase banding patterns under reducing conditions from whole cell lysates of four axenic and four xenic pathogenic strains of entamoeba histolytica. all strains shared major bands in the 34 and 66-68 kda regions, whereas only the axenic strains produced major bands at 26, 28, 30 and 45 kda. one axenic strain, nih 200, when reassociated with mixed bacterial flora, reverted to an electrophoretic banding pattern characteristic ... | 1993 | 8225772 |
| pharmacokinetics and amoebicidal activity of (+-)-(e)-3-(4- methylsulphinylstyryl)-1,2,4-oxadiazole (bti 2286e) and its sulphone metabolite (bti 2571e) in the golden hamster, mesocricetus auratus. | bti 2286e is a 1,2,4-oxadiazole with amoebicidal activity. three groups of golden hamsters received single doses of bti 2286e or its sulphone metabolite bti 2571e as either bti 2286e 60 mg/kg po or bti 2571e 60 mg/kg po or bti 2571e 60 mg/kg ip. blood samples were collected up to 8 h post-dose and plasma concentrations of bti 2286e and bti 2571e were assayed by hplc. bti 2286e was rapidly absorbed, extensively metabolized during first pass and rapidly eliminated with a plasma elimination half-li ... | 1993 | 8226401 |
| structural analysis of the light subunit of the entamoeba histolytica galactose-specific adherence lectin. | adherence of entamoeba histolytica trophozoites to colonic mucins and resistance to lysis by the membrane attack complex of complement are mediated by a galactose- and n-acetyl-d-galactosamine-specific cell-surface lectin. this lectin is a heterodimeric glycoprotein of heavy (170 kda) and light (35/31 kda) subunits. in this work, the amino acid sequence and membrane anchor of the light subunit were analyzed. the light subunit cdna encoded a protein with a calculated molecular mass of 32 kda cont ... | 1993 | 8226970 |
| degradation of human iga by entamoeba histolytica. | to determine whether the virulent enteric pathogen entamoeba histolytica degrades human iga molecules, serum and secretory iga was exposed to viable axenic trophozoites (strain hm1:imss), a parasite sonicate, and medium conditioned by incubation with live trophozoites. iga was completely degraded under all conditions, proteinase activity was maximal at a neutral ph, and there was a four- to eightfold enrichment of amebic iga proteolytic activity in a soluble fraction of amebic sonicate. degradat ... | 1993 | 8228372 |
| cerebral amoebiasis. | 1993 | 8230659 | |
| molecular cloning of a rac family protein kinase and identification of a serine/threonine protein kinase gene family of entamoeba histolytica. | eleven entamoeba histolytica protein-serine/threonine-kinase gene segments were identified using the polymerase chain reaction (pcr) and degenerate oligonucleotide primers to conserved amino acids in subdomains vi and viii of the catalytic domain of protein-serine/threonine kinases. these ameba gene segments were homologous to myosin light chain kinases, protein kinase c, phosphorylase b kinase, and kinases that regulate glucose repression in yeast and cell growth in mammalian cells. one of thes ... | 1993 | 8232409 |
| the cdna sequence of an abundant entamoeba histolytica 20-kilodalton protein containing four repetitive domains. | 1993 | 8232422 | |
| [amebic brain abscess. apropos of a case]. | we report a case of double primary amoebic brain abscess in a 22 year old man, with signs of intracranial hypertension and deficit. computed tomography found two hypodense lesions, not suggestive of pyogenic abscesses. aspiration of one of the two abscesses, revealed the organism, and the two abscesses regressed in response to medical treatment. the authors insist on the rarity of these abscesses, their gravity, and the difficulty of the diagnosis. | 1993 | 8239477 |
| design, synthesis and biological study of new antiparasitic spiroarsoranes. | thirty-eight new spiroarsoranes were synthesized after structure-activity relationship studies from the first series. these compounds were predicted to cross more easily the membrane of protozoae or the cuticle of nematodes and to reach their biological target with efficiency. the spiroarsoranes were evaluated for their antiparasitic properties, on helminths and protozoa models in regard of their parent arsonic acids. the following parasite models were used: entamoeba histolytica and trichomonas ... | 1993 | 8240448 |
| a variable dna region of entamoeba histolytica is expressed in several transcripts which differ in genetically related clones. | a highly variable dna region (ehvr1), isolated from entamoeba histolytica clone a, strain hm1:imss, is transcribed into several transcripts, which differ in genetically related clones. ehvr1 (3.5 kb) is composed of two contiguous fragments; one of these 1.9 kb long, at the 3' end, identified similar transcripts in clones a, l6 and c2 (all derived from strain hm1:imms), the other of 1.6 kb, at the 5' end, detected 0.5, 0.6 and 0.7 kb transcripts only in clone a. variability of the 1.6 kb fragment ... | 1993 | 8246880 |
| entamoeba histolytica: identification of a major surface antigen in liver abscess pus using a monoclonal antibody. | 1993 | 8249093 | |
| adherence of entamoeba histolytica to hydrophobic matrices: a simple method for measuring cell surface hydrophobicity. | 1993 | 8249095 | |
| entamoeba histolytica: a method for isolate identification. | the ability to identify individual isolates of entamoeba histolytica schaudinn 1903 (emend. walker 1911) is necessary before several important epidemiological questions can be answered. we have developed such a method based on our discovery of extensive polymorphism in two e. histolytica genes--the serine-rich antigen gene and the "strain specific gene"--each of which has an internal tandemly repeated structure. using the polymerase chain reaction we detected both size and restriction site polym ... | 1993 | 8253158 |
| entamoeba histolytica: an explanation for the reported conversion of "nonpathogenic" amebae to the "pathogenic" form. | the reported conversion of "nonpathogenic" entamoeba histolytica isolates to the "pathogenic" form during attempted axenization of the amebae is highly controversial. after failing to obtain conversions ourselves we concluded that the simplest explanation for the published observations would be contamination of nonpathogenic cultures with pathogenic amebae. to address this possibility we used a method based on analysis of stable dna polymorphisms that allows the positive identification of indivi ... | 1993 | 8253159 |
| [drinking water and parasites]. | entamoeba histolytica, giardia lamblia, cryptosporidium parvum, isospora belli, balantidium coli, and microsporidia spp. are cosmopolitan parasites. they often cause diarrheal diseases. the waterborn transmission of all these parasites is possible (41). surface water supplies used for drinking water are potential sources of contamination. giardia lamblia and cryptosporidium spp. have received great attention in industrialized countries during the last years because they are the etiological agent ... | 1993 | 8253478 |
| amebiasis and "nonpathogenic" intestinal protozoa. | infection with single or multiple species of intestinal protozoa is common in humans and can result in either asymptomatic colonization or symptoms of intestinal disease. entamoeba histolytica serves as a paradigm for invasive colonic protozoal infection. the key to diagnosis and treatment of amebiasis is knowledge of the epidemiologic risk factors and clinical manifestations, a rational approach to diagnosis, and an understanding of the sites of action and uses of anti-amebic drugs. this knowle ... | 1993 | 8254155 |
| [ameboma of the large intestine and rectum]. | amoeboma was diagnosed in three patients: a bengal seaman aged 40 and two dutch citizens who had lived in the tropics, a man aged 41 and a woman aged 56. the first-mentioned patient had a rectal tumour easily inspected by sigmoidoscopy; a biopsy sample contained entamoeba histolytica. the faeces contained no cysts. the two other patients had coecal amoebomas. they had been operated elsewhere on suspicion of appendicitis and had been treated with metronidazole, which had failed to eliminate the a ... | 1993 | 8255341 |
| schistosomiasis and intestinal parasites in rural villages in southwest nigeria: an indication for expanded programme on drug distribution and integrated control programme in nigeria. | a cross sectional survey of schistosomiasis, intestinal parasite infections and pattern of schistosomiasis transmission was undertaken in four rural villages around the erinle dam, nigeria. the prevalence of schistosoma haematobium ranged from 10% to 60%. 30% of infected school children excreted over 50 eggs/10 ml urine and high rates of haematuria, proteinuria, leucocyturia and nitrites in urine were observed in infected children and the villagers. prevalence of infection with s. mansoni ranged ... | 1993 | 8256092 |
| the proteases and pathogenicity of parasitic protozoa. | protozoan parasites are among the most prevalent pathogens worldwide. diseases like malaria, leishmaniasis, amebiasis, and trypanosomiasis affect hundreds of millions of people. recent advances in our understanding of the biochemistry and molecular biology of these organisms has focused attention on specific parasite molecules that are key to the parasite life cycle or the pathogenesis of the diseases they produce. one group of enzymes that plays myriad roles in these processes are the parasite- ... | 1993 | 8257117 |
| [fatal transmural colitis caused by entamoeba histolytica. differential diagnosis of chronic inflammatory bowel disease and colon cancer]. | perforation of the colon is a rare but frequently fatal complication of amoebiasis. we report a case of a 53 year-old male, with no history of travel abroad, who was admitted to hospital with haematochezia. a tumor of the rectum was diagnosed clinically. due to acute intestinal obstruction, laparotomy was performed, revealing multiple perforations of the large bowel and severe peritonitis leading to subtotal colectomy. the histological examinations revealed transmural amoebic colitis. the patien ... | 1993 | 8259618 |
| molecular and cell biology of opportunistic infections in aids. entamoeba histolytica. | 1993 | 8261181 | |
| differentiation of pathogenic entamoeba histolytica infections from nonpathogenic infections by detection of galactose-inhibitable adherence protein antigen in sera and feces. | we determined whether epitope-specific monoclonal antibodies to the galactose-inhibitable adherence protein (giap) of entamoeba histolytica could be used in an enzyme-linked immunosorbent assay (elisa) to detect antigen in serum and feces and differentiate between nonpathogenic zymodemes and the potentially invasive pathogenic organisms that require treatment. overall, 57% of subjects from cairo, egypt, with symptomatic intestinal amebiasis and 42% with asymptomatic infection possessed giap anti ... | 1993 | 8263165 |
| sequence analysis of genes encoding the light subunit of the entamoeba histolytica galactose-specific adhesin. | 1993 | 8264736 | |
| intestinal parasitic infections in campalagian district, south sulawesi, indonesia. | intestinal parasitic infections were surveyed in the inhabitants of 3 coastal and 2 inland villages of campalagian district, south sulawesi, indonesia, in july 1992. a total of 398 fecal samples were examined by using kato-katz thick smear, harada-mori culture and agar-plate culture techniques. protozoan cysts were examined by formalin ether concentration technique on 380 fecal samples. soil-transmitted helminth infections were highly prevalent with the overall positive rates as follows: ascaris ... | 1993 | 8266235 |
| unusual gene organization in the protozoan parasite entamoeba histolytica. | we have analyzed three independent genomic loci of the protozoan parasite entamoeba histolytica that contain coding regions for the iron-containing superoxide dismutase, the pore-forming peptide, and the galactose-inhibitable lectin. all of the three structural genes were found to be closely linked unidirectionally to other coding sequences. the intergenic regions did not exceed 1,350 nucleotides. nuclear run-on data demonstrated that at least the galactose-inhibitable lectin gene is transcribed ... | 1993 | 8274224 |
| entamoeba histolytica: inhibition of malic enzyme and alcohol dehydrogenase by (+/- )-, (+)-, and (-)- gossypol. | gossypol, a natural racemic mixture with action on nadp- and nad-oxidoreductases from diverse species, has been proposed as a possible antiamebic medication considering several of its pharmacological properties. in this study it was found that malic enzyme and alcohol dehydrogenase from entamoeba histolytica are strongly inhibited by (+/-)-gossypol, and both (+)- and (-)- enantiomers. the inhibition was of the noncompetitive type among their respective substrates in all cases. the (+/-), (+)-, ( ... | 1993 | 8274846 |
| pathogenicity of entamoeba histolytica isolates from shanghai, china. | 1993 | 8278345 | |
| the occurrence of human intestinal parasites in finland. | the survey of the occurrence of intestinal parasites presented here covers 726 hospital patients, 175 healthy adults and 212 children, and 976 healthy finnish holiday-makers prior to their trip abroad. two faecal samples from each individual were examined. further, 344 children were tested for enterobius vermicularis. the total prevalence of intestinal parasites in the hospital patients was 4.2%, and that of pathogenic parasites 2.9%. the prevalence rates in healthy subjects were 4.9% and 1.5%, ... | 1993 | 8284655 |
| a fluorescence-based quantitative adhesion assay to study interactions between entamoeba histolytica and human enterocytes. effect of proinflammatory cytokines. | in order to study the initial events during infection of target cells by the enteric pathogen entamoeba histolytica, we developed a quantitative adhesion assay based on the use of a human colonic cell line (caco-2) and biotinylated amoebae tagged with fluorescein. to prevent the strong and rapid lytic activity of entamoeba histolytica on colonic cells, which would otherwise impede the study of the primary adhesion steps, parasites were mildly fixed, biotinylated and labelled with streptavidin-fi ... | 1993 | 8288877 |
| a study of the aetiological agents of childhood diarrhoea in lagos, nigeria. | from december 1989 to may 1990, 315 faecal samples from children under 5 years old with diarrhoea (215) and without diarrhoea (100) seen at paediatric clinics were investigated for bacterial, viral and parasitic enteropathogens. standard and recently described methods were used for the investigations, which revealed that 74.9% of children with diarrhoea were infected with enteropathogens compared with 28% of controls. in the diarrhoeal group, 59.1% had a bacterial, 26.5% a viral and 2.3% a paras ... | 1994 | 8289209 |
| production and characterization of monoclonal antibodies against a highly immunogenic fraction of entamoeba histolytica (nih:200) and their application in the detection of current amoebic infection. | six monoclonal antibodies (mabs) were produced against a highly immunogenic fraction derived by the chromatographic separation of the soluble preparation of axenic entamoeba histolytica (strain nih:200) trophozoites. isotype characterization of the six mabs revealed that four belonged to the igm class and one each to the igg1 and the igg2a subclasses. the immunoreactivity patterns and the specificity of the mabs with homologous and heterologous antigens were analyzed by the enzyme-linked immunot ... | 1993 | 8292992 |
| amoebic liver abscess--an unusual presentation. | 1993 | 8294371 | |
| correlation between membrane glycoprotein and detergent dissected membrane protein in the assessment of lmif: a report of 51 ala cases. | assessment of 51 amoebic liver abscess cases for leukocyte migration inhibition factor released using membrane glycoprotein and detergent dissected membrane protein (ddmp) of axenic entamoeba histolytica (nih:200). lymphokines release by t lymphocytes in response to purified amoebic membrane glycoprotein (pamg) against whole amoebic lysate (wal), dissect out protein against whole amoebic lysate and membrane glycoprotein against dissected protein was tested by leukocyte migration inhibition test ... | 1993 | 8300172 |
| human secretory immunoglobulin a anti-entamoeba histolytica antibodies inhibit adherence of amebae to mdck cells. | the presence of secretory immunoglobulin a (iga) anti-entamoeba histolytica antibodies in the saliva of patients with intestinal amebiasis was demonstrated by immunoblot assay, and the capacity of these antibodies to inhibit amebic adherence to a monolayer of mdck cells was analyzed. inhibition was due to iga antiamebic antibodies and in part to anti-gal-binding-lectin antibodies, as demonstrated by absorption experiments with total amebic extract and with the fraction of gal-binding lectin. the ... | 1994 | 8300242 |
| cutaneous amebiasis in children. | a 7-month-old girl developed cutaneous amebiasis of the perianal and genital areas in association with amebic dysentery. the diagnosis was based on the identification of entamoeba histolytica by skin biopsy. she was treated with dehydroemetine and metronidazole with excellent results. this is only the eighth reported case of this disease in a child. | 1993 | 8302738 |
| the microbiology of acute diarrhoeal disease in the eastern province of saudi arabia. | a prospective study of acute diarrhoeal diseases in the eastern province of saudi arabia was carried out over a 19-month period to determine aetiology, risk factors and other epidemiological characteristics. of the 853 subjects studied, 344 were cases and 509 controls. more cases were seen in children than in adults. enteric pathogens were detected in 49% of the cases, but none in the controls. of the pathogens, 68% were bacterial with salmonella (34%) and shigella species (14.7%) being the most ... | 1993 | 8306900 |
| serologic reactivity to purified recombinant and native 29-kilodalton peripheral membrane protein of pathogenic entamoeba histolytica. | the 29-kda peripheral membrane protein of entamoeba histolytica has recently been demonstrated to have epitopes on pathogenic clinical isolates which were not detected by monoclonal antibodies on nonpathogenic isolates. to analyze the serological response to this protein, we tested 93 serum specimens (from 33 patients with amebic liver abscess, 7 patients with colitis, 2 patients with ameboma, 18 individuals harboring a nonpathogenic zymodeme strain, 10 healthy mexican migrant workers, and 23 he ... | 1993 | 8314979 |
| campylobacter-associated diarrhoea in egyptian infants: epidemiology and clinical manifestations of disease and high frequency of concomitant infections. | a diarrhoeal disease survey in alexandria, egypt determined the prevalence, seasonality, and household risk factors for campylobacter-associated diarrhoea in young children. the study population was 880 children (mean age = 9.8 months) presenting with diarrhoea at one of two hospitals. a control group consisted of 1,079 healthy children (mean age = 8.8 months) attending two nearby vaccination clinics. the overall isolation frequencies for campylobacter spp. were 16.8% for cases and 6.4% for the ... | 1993 | 8315255 |
| early branchings in the evolution of eukaryotes: ancient divergence of entamoeba that lacks mitochondria revealed by protein sequence data. | phylogenetic analyses of ribosomal rna sequences have played an important role in the study of early evolution of life. however, loomis and smith suggested that the ribosomal rna tree is sometimes misleading--especially when g+c content differs widely among lineages--and that a protein tree from amino acid sequences may be more reliable. in this study, we analyzed amino acid sequence data of elongation factor-1 alpha by a maximum likelihood method to clarify branching orders in the early evoluti ... | 1993 | 8315658 |
| 31p-nmr analysis of entamoeba histolytica. occurrence of high amounts of two inositol phosphates. | perchloric-acid extracts of axenic entamoeba histolytica were investigated by 31p-nmr spectroscopy. all major 31p resonances observed were assigned to specific compounds. the cells contained inorganic phosphate (1039 nmol/g wet cells), pyrophosphate (16 nmol/g wet cells), nucleoside diphosphates (91 nmol/g wet cells), nucleoside triphosphates (275 nmol/g wet cells), nad(p) (60 nmol/g wet cells), phosphocholine (184 nmol/g wet cells), phosphoethanolamine (214 nmol/g wet cells), cytidine 5'-diphos ... | 1993 | 8319681 |
| interaction between facilitated diffusion of glucose across the plasma membrane and its metabolism in trichomonas vaginalis. | the parasitic protist trichomonas vaginalis transports glucose across the plasma membrane by facilitated diffusion. the km of the transporter for glucose was 1.6 mm. the uptake of labelled glucose in a minimal medium not allowing growth reached saturation only after 2.5 h, indicating the turnover of storage carbohydrate. organisms grown on glucose showed higher activities both of the transporter and of the subsequent metabolic pathway than organisms grown on maltose. at low external glucose conc ... | 1993 | 8319891 |
| effect of recombinant tumor necrosis factor alpha on in vitro amoebicidal activity of murine kupffer cells. | the present study was undertaken to determine whether recombinant tumor necrosis factor alpha (tnf) could induce kupffer cells to kill entamoeba histolytica parasite in vitro. c57bl/6 mice were used in this study. the liver was perfused and kupffer cells harvested and treated with tnf for 6 h. it was found that kupffer cells treated with tnf are able to kill amoebic trophozoites in vitro. these results further show that amoebicidal activity of tnf-activated kupffer cells is dependent on the rati ... | 1993 | 8326852 |
| [carriers and excretors of protozoa]. | causative agents of intestinal infections in humans are about 10 pathogenic or facultative pathogenic species of protozoa of which giardia lamblia, entamoeba histolytica, cryptosporidium parvum and enterozytozoon bieneusi are discussed. g. lamblia from humans is morphologically indistinguishable from giardia isolates originating from several species of domestic and wild mammals. swiss giardia isolates of human, sheep, cattle and dog origin could be transmitted to giardia-free rodents and were ra ... | 1993 | 8333895 |
| electrophoretic karyotype and chromosome assignments for a pathogenic and a nonpathogenic strain of entamoeba histolytica. | the electrophoretic karyotypes of a pathogenic and a nonpathogenic strain of entamoeba histolytica were determined by pulsed-field gel electrophoresis. a number of previously isolated genes were assigned to specific chromosomal bands. significant differences between the chromosomal patterns of these strains as well as in the assignment of most genes were found. | 1993 | 8335392 |
| predictive value of diagnostic tests and prevalence of invasive entamoeba histolytica infection. | 1993 | 8335998 | |
| human immunodeficiency virus and leprosy. | in summary, clinical leprosy does not appear to be more frequent in hiv-positive than in hiv-negative people in areas where both infections are endemic. there is no evidence that the paucibacillary to multibacillary distribution of patients is altered by hiv infection. there are reports that neuritis is more severe in co-infected people, and that reversal reactions (or, at least, new lesions) may be more frequent after therapy; but these reports are either poorly documented or poorly controlled. ... | 1993 | 8341127 |
| pathogenic entamoeba histolytica: cdna cloning of a histone h3 with a divergent primary structure. | entamoeba histolytica has an unusual nuclear structure characterized by a low degree of chromatin condensation and the absence of stainable metaphase chromosomes. although nucleosome-like particles were observed, no information about histones was available so far. in this paper we describe a cdna clone with significant homology to h3 histones that was isolated from a library of pathogenic e. histolytica. the complete cdna encodes a 15-kda polypeptide, which like the histone sequence from volvox ... | 1993 | 8341328 |
| expression of a recombinant entamoeba histolytica antigen in a salmonella typhimurium vaccine strain. | the expression of a major surface antigen of the intestinal protozoal parasite entamoeba histolytica in an attenuated salmonella typhimurium vaccine strain is described. a polymerase chain reaction fragment derived from cdna encoding the serine-rich entamoeba histolytica protein, srehp, was introduced into s. typhimurium chi 3987 (delta cya delta crp delta asd) using a plasmid expression vector (pya292) containing the aspartate semialdehyde (asd) gene. s. typhimurium expressing recombinant srehp ... | 1993 | 8342325 |
| entamoeba histolytica: the ehzc3 cdna clone encodes a zinc-binding protein. | 1993 | 8344401 | |
| improved method for the concentration and purification of faecal cysts of entamoeba histolytica for use as antigen. | human stool samples are the most widely used source of e. histolytica cysts for diagnosis and research. cysts concentrated from faecal samples are contaminated with faecal debris and bacteria and are unsuitable for use as antigens. other gradient purification and sedimentation procedures result in considerable cyst losses. we present an effective and simple purification procedure using varying densities of percoll that yields an adequate cyst concentration from a moderate cyst load in a single s ... | 1993 | 8345546 |
| entamoeba histolytica: antibody response to recent and past invasive events. | sero-epidemiological data from endemic amoebiasis areas are difficult to evaluate because the serology of individuals affected by an active process of entamoeba histolytica tissue invasion is, at present, almost impossible to distinguish from that of individuals who have had an invasive event in the past. the present study compares serum antigenic recognition frequencies among three groups of individuals with different infective conditions: amoebic liver abscess patients; asymptomatic cyst passe ... | 1993 | 8346990 |
| newer stool based diagnostic tests in infectious diseases: possibilities and pitfalls. | 1993 | 8354530 | |
| detection of entamoeba histolytica antigens in stool in amebiasis. | stool microscopy, the conventional method of diagnosing intestinal amebiasis, fails to detect entamoeba histolytica in more than 30-40% of clinically suspected cases. demonstration of parasite products in clinical specimens has been suggested as an alternative. however, the usefulness of demonstrating amebic antigen in the stools of clinical cases needs to be assessed. | 1993 | 8354532 |
| imported opisthorchis viverrini and parasite infections from thai labourers in taiwan. | in order to determine the prevalence of intestinal parasites among labourers from thailand in taiwan, the stool samples from 1364 thai labourers were examined by the formalin-ether concentration method and 18.0% were found to be infected with one or more parasites. the infection rates of hookworm, opisthorchis viverrini, strongyloides stercoralis, enterobius vermicularis, ascaris lumbricoides, hymenolepis nana, capillaria philippinensis, schistosoma japonicum, echinostoma sp., entamoeba coli, gi ... | 1993 | 8354853 |
| correlation between antiamoebic igg and autoreactive anti-igg in amoebic liver abscess cases. | igg isolated and purified from a healthy human serum through sephadex g-200 and protein a cl 4b sepharose chromatography was used for detection of its own antibodies and correlated with the antiamoebic antibody titres in amoebic liver abscess cases. the mean titres with standard deviation of the self reactive antibodies to serum igg both ala cases and healthy controls show a highly significant difference, and antiamoebic antibody titres (igg) are very much correlated with the autoreactive anti i ... | 1993 | 8359844 |
| recognition of entamoeba histolytica in liver abscess cases through their specific antiamoebic antibodies. | 1993 | 8359857 | |
| serum-independent and serum-dependent cytoadherence in the interaction of entamoeba histolytica with mammalian target cells. | entamoeba histolytica kills target cells only on direct contact, suggesting that trophozoite-mediated cytolysis is initiated by the contact between trophozoites and target cells. we have shown that adherence between e. histolytica and target cells (polymorphonuclear granulocytes, erythrocytes, chinese hamster ovary cells, human colon carcinoma cells) was inhibited by specific carbohydrates, and adherence between e. histolytica and polymorphonuclear granulocytes (pmn) was enhanced by preincubatio ... | 1993 | 8359985 |
| some characteristics of hospitalized hiv seropositive patients in myanmar. | twenty-two hospitalized hiv seropositive patients were studied prospectively between july 1991 and january 1992. the majority of the patients were intravenous drug users (ivdus). their age ranged from 20 to 38 years with a male preponderance of 12 to 1. anemia, lymphopenia and thrombocytopenia were observed in 100%, 36% and 41%, respectively. the common pathogens like malaria parasites, mycobacterium tuberculosis, entamoeba histolytica, streptococcus and salmonella were isolated/identified rathe ... | 1993 | 8362294 |
| analysis of the genomic sequence encoding the 29-kda cysteine-rich protein of entamoeba histolytica. | by analyzing cdna and genomic clones coding for the 29-kda cysteine-rich protein of entamoeba histolytica, substantial sequence differences were found to the 5'-end of a previously described full-length cdna coding for the same protein (reed et al., 1992), which was reported to contain an untranslated 5'-sequence of at least 171 nucleotides, unusual for e. histolytica cdnas. we found evidence that the cdna published by reed et al. (1992) represents a hybridclone composed of two unrelated sequenc ... | 1993 | 8367659 |
| diagnosis of pathogenic entamoeba histolytica infection using a stool elisa based on monoclonal antibodies to the galactose-specific adhesin. | monoclonal antibodies (mabs) directed against pathogen-specific epitopes of the galactose adhesin of entamoeba histolytica were used in an elisa to detect antigen from pathogenic e. histolytica. single stool specimens from 74 patients in bangladesh were used. the elisa for pathogenic e. histolytica was positive in all 12 stool specimens with pathogenic amebae subsequently cultured, in no stool specimens with nonpathogenic e. histolytica and in 2 of 40 stools with other or no intestinal parasites ... | 1993 | 8369019 |
| synthesis, antibacterial and antifungal activity of metal (ii) complexes of 2-acetylpyridine thiosemicarbazones. | 2-acetylpyridine-(4-methylthiosemicarbazone), 2-acetylpyridine-(2- methylthiosemicarbazone) and their metal (ii) complexes have been synthesized and characterized by chemical and spectral methods. they were studied for their antibacterial, antifungal and amoebicidal activity in vitro and for their toxicity in vivo. the ligands and their metal (ii) complexes exhibited significant activity against a wide spectrum of microorganisms. the metal (ii) complexes derived from 2-acetylpyridine-(4-methylth ... | 1993 | 8373503 |
| entamoeba histolytica: electrophysiologic and morphologic effects of trophozoite lysates on rabbit colon. | although in acute intestinal amoebiasis the events occurring during the initial interaction of entamoeba histolytica trophozoites with the mucosa of the large intestine probably determine the invasion by the parasites, an appropriate experimental model to study them does not exist. to develop such a model we used rabbit colonic segments (0.28 cm2) mounted in ussing-type chambers. these preparations had electrophysiological properties (potential difference, short-circuit current, and electrical r ... | 1993 | 8375485 |
| entamoeba histolytica proteins modulate the respiratory burst potential by murine macrophages. | reactive oxygen intermediates are important components of macrophage microbicidal mechanisms and pathogenesis of parasitic disease. the purpose of the present study was to investigate the effect of virulent entamoeba histolytica (strain hm1-imss) on respiratory burst potential of macrophages. pretreatment of elicited peritoneal macrophages (epm) with crude soluble amoebic proteins from 1 to 6 hr was found to prime epm for enhanced o2 and h2o2 release in response to phorbol myristate acetate (pma ... | 1993 | 8386134 |
| electron transport components of the parasitic protozoon giardia lamblia. | the energy metabolism of the intestinal parasite, giardia lamblia, involves the iron-sulphur protein, pyruvate:ferredoxin oxidoreductase. cell fractionation studies showed that this enzyme is associated with the membranes. nadh and nadph dehydrogenases were found in both the membrane and cytosolic fractions. epr spectroscopic studies showed the presence of iron-sulphur clusters in the membrane fraction and in the cytosolic fraction, non-sedimentable at 6 x 10(6) g.min. an acidic, soluble protein ... | 1993 | 8391475 |
| entamoeba histolytica cyst passers. clinical profile and spontaneous eradication of infection. | the present study was carried out to examine whether entamoeba histolytica cyst passers suffer from any parasite-related bowel symptoms and to assess the frequency of spontaneous eradication of this infection. the study was carried out in two parts. in part i, stool samples were collected at random from 3536 individuals living in rural communities around delhi. e. histolytica was isolated by the culture technique in 345 (9.7%) subjects. there was no increase in the prevalence rate of bowel sympt ... | 1993 | 8404403 |
| in vitro effect of recombinant interferon gamma in combination with lps on amoebicidal activity of murine kupffer cells. | the present study examines the role of liver macrophages (kupffer cells), of c57bl/6 mice, as effector cells responsible for the killing of entamoeba histolytica trophozoites in vitro. it was shown that unstimulated kupffer cells were inefficient in the killing of e. histolytica trophozoites in vitro. interferon gamma (ifn-gamma) alone was not able to activate kupffer cells to amoebicidal state. however, interferon gamma and lipopolysaccharide (lps) acted synergistically in this phenomenon. it s ... | 1993 | 8406558 |
| in vitro encystation and excystation of entamoeba histolytica trophozoites. | 1993 | 8406604 | |
| detection of sialic acid in trophozoites of entamoeba histolytica (nih:200). | in two successive experiments detection of sialic acid was achieved from trophozoites of axenically grown e. histolytica (nih:200) using silicic acid column chromatography for the separation of sialosylated lipids and asialosylated lipids from total lipids. sialic acid of the sialosylated lipids was detected through thiobarbituric acid assay followed by acid hydrolysis. these findings indicate that presence of sialic acid is not only a character of the cystic stage but also that of trophozoites ... | 1993 | 8406633 |
| isoenzyme pattern of clones of entamoeba histolytica. | the isoenzyme pattern of clones of e. histolytica isolated from symptomatic and asymptomatic patients of amoebiasis were determined by starch gel electrophoresis. ten parent cultures (uncloned), 5 from symptomatic and 5 from asymptomatic patients were isolated and established in robinsons medium as xenic cultures. a total of 100 clones were isolated from parent cultures but only 65 could be established. starch gel electrophoresis was carried out on the uncloned and cloned cultures. the analysis ... | 1993 | 8406646 |
| entamoeba histolytica: changes in the zymodeme of cloned nonpathogenic trophozoites cultured under different conditions. | in this paper we report the occurrence of changes in the migration of certain isoenzymes of a cloned strain (mav-cinvestav) of entamoeba histolytica. this strain was isolated from an asymptomatic carrier and showed an initial nonpathogenic zymodeme i. the transfer of polyxenic trophozoites from robinson's medium (7% serum) to jones' medium (30% serum) provoked changes in isoenzyme expression, resulting in the conversion of zymodeme i to a zymodeme that was similar to the xvii zymodeme except for ... | 1993 | 8415539 |
| biochemical and immunological studies on soluble antigens of entamoeba histolytica. | the soluble antigens of entamoeba histolytica trophozoites were analysed in detail by biochemical and immunochemical methods. the antigen was highly complex and heterogeneous as revealed by sephacryl s-300 column chromatography, which showed four distinct fractions. the molecular mass of fractions fi, fii, fiii and fiv was 660, 170, 65 and 13 kda, respectively. protein was the major constituent in crude soluble antigen (csa) and fractions fi and fii (67%, 80% and 90%, respectively). polysacchari ... | 1993 | 8415541 |
| dose- and time-dependent functional and structural damage to the colon mucosa by entamoeba histolytica trophozoite lysates. | analysis of the initial interaction between entamoeba histolytica trophozoites and the large intestine is impossible in humans and very difficult in experimental animals. to circumvent this obstacle we treated the luminal side of full-thickness rabbit colon segments mounted in ussing-type chambers with trophozoite lysates of the e. histolytica hm1 virulent strain. exposure to lysates for up to 90 min produced dose- and time-dependent effects on the colon, consisting of (a) increased decay rates ... | 1993 | 8415570 |
| role of infectious agents in exacerbations of ulcerative colitis in india. a study of clostridium difficile. | fifty patients with idiopathic ulcerative colitis--25 with acute exacerbation of the disease (group i) and 25 in quiescent phase (group ii)--were studied. none of the patients had a history of recent exposure to antimicrobial drugs or hospitalization. evidence of infection with protozoal and bacterial agents and/or presence of clostridium difficile toxin was demonstrated in eight (32%) patients in group i and one (4%) patient in group ii (group i vs. group ii, p < 0.05; chi 2 test with yate's co ... | 1993 | 8421140 |
| molecular characterization of the cdna coding for translation elongation factor-2 of pathogenic entamoeba histolytica. | to investigate the humoral immune response of patients with amoebic dysentry against entamoeba histolytica, immunoglobulin g (igg)-immunopositive cdna clones from the pathogenic strain sfl-3 were examined. a large part of the igg-positive cdna clones obtained with one serum encoded highly conserved intracellular proteins. a clone was found that was homologous to translation elongation factor-2 (ef-2). sequence analysis of the ef-2 cdna showed 63.6% amino acid sequence identity with the human hom ... | 1993 | 8422275 |
| the ca(2+)-atpase activity of entamoeba histolytica is exposed towards the medium and towards the lumen of intracellular vesicles. | 1993 | 8426610 | |
| application of the polymerase chain reaction to the epidemiology of pathogenic and nonpathogenic entamoeba histolytica. | we used the polymerase chain reaction (pcr) to study the epidemiology of pathogenic and nonpathogenic entamoeba histolytica in a rural community in mexico. formalin-fixed stool samples were used for extraction of dna. the pcr amplifications were performed using two sets of primers that discriminate between pathogenic or non-pathogenic e. histolytica. a total of 201 randomly selected individuals were studied. among them, 25 (12%) were diagnosed to be infected with e. histolytica by microscopy; pc ... | 1993 | 8427389 |
| localization of myosin heavy chain a in the human pathogen entamoeba histolytica. | to recognize myosin ii in trophozoites of the human pathogen entamoeba histolytica, a specific antimyosin polyclonal serum was raised against a fusion protein consisting of a 146-amino-acid fragment of the myosin ii heavy chain a of e. histolytica (mhca) fused with beta-galactosidase. the hybrid protein was encoded by a chimera gene formed by a dna fragment, from the mhca gene, amplified by polymerase chain reaction and fused with the lacz gene of escherichia coli. polymerase chain reaction-ampl ... | 1993 | 8432587 |
| [febrile status in a traveler returning from the tropics with right-sided upper abdominal pain]. | this is a case report of a patient who presented multiple liver abscesses with entamoeba histolytica five weeks after returning from an one-month trip to thailand. this makes clear that a febrile patient returning from the tropics may not only be suspected of malaria, but that we should think about other possible febrile diseases. respecting the incubation period and other clinical symptoms and signs, we are in most of the cases able to reduce the differential diagnosis to only a few possible on ... | 1993 | 8434207 |
| role of cysteine proteinases of entamoeba histolytica on the cytopathogenicity of axenic trophozoites on rat and hamster hepatocytes in vitro. | the role of amebic cysteine proteinase(s) on the in vitro cytotoxic and cytolytic effects of axenic trophozoites of a virulent strain (hm-1) of entamoeba histolytica has been studied using freshly isolated rat and hamster hepatocytes as target cells. the cytotoxic effect was defined as cell killing without loss of cell structure and the cytolytic effect as cell disintegration. incubation experiments of axenic trophozoites with rat or hamster liver cells in the presence and absence of bovine seru ... | 1993 | 8437063 |
| antigenicity, immunogenicity and vaccine efficacy of the galactose-specific adherence protein of entamoeba histolytica. | entamoeba histolytica is an enteric protozoan that causes amoebic colitis and liver abscess. human immunity to e. histolytica is apparently mediated by a serum antibody response and amoebicidal cellular mechanisms. the galactose-specific adherence protein of e. histolytica is a 260 kda glycoprotein which mediates amoebic in vitro adherence to human colonic mucins, epithelium, and inflammatory cells. amoebic lysis of cells is dependent upon binding by this adherence protein. serum igg and salivar ... | 1993 | 8438621 |
| intestinal parasitic infections and urbanization. | about a third of the population in the cities of developing countries live in slums and shanty towns. by the year 2000 it is estimated that this number will grow to 2200 million, and by 2025 about 57% of the population in developing countries will be in urban areas. the prevalence of infections caused by entamoeba histolytica and giardia intestinalis and the prevalence and intensity of ascaris lumbricoides and trichuris trichiura infections may increase among the rural populations who are migrat ... | 1993 | 8440028 |