Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| chemical composition and antibacterial activity of lavandula coronopifolia essential oil against antibiotic-resistant bacteria. | the aim of this study was to analyse the composition of the essential oil (eo) of lavandula coronopifolia from morocco and to evaluate its in vitro antibacterial activity against antibiotic-resistant bacteria isolated from clinical infections. the antimicrobial activity was assessed by a broth micro-well dilution method using multiresistant clinical isolates of 11 pathogenic bacteria: klebsiella pneumoniae subsp. pneumoniae, klebsiella ornithinolytica, escherichia coli, enterobacter cloacae, ent ... | 2015 | 25174508 |
| carbapenem resistance among enterobacter species in a tertiary care hospital in central india. | objective. to detect genes encoding carbapenem resistance among enterobacter species in a tertiary care hospital in central india. methods. bacterial identification of enterobacter spp. isolates from various clinical specimens in patients admitted to intensive care units was performed by routine conventional microbial culture and biochemical tests using standard recommended techniques. antibiotic sensitivity test was performed by standard kirby bauer disc diffusion technique. pcr amplification a ... | 2014 | 25180095 |
| physico-chemical analysis and antimicrobial potential of apis dorsata, apis mellifera and ziziphus jujube honey samples from pakistan. | to evaluate physico-chemical properties and antimicrobial potential of indigenous honey samples against different reference strains including escherichia coli atcc 8739, enterobacter aerogenes atcc 13048, pseudomonas aeroginosa atcc 9027, bacillus subtilis atcc 6633, staphylococcus aureus atcc 25923, salmonella typhi atcc 14028, klebsiella pneumonia atcc 13883, aspergillus niger atcc 16404, rhizopus oligosporus pcsir1, candida albicans atcc 14053 and candida utilis atcc 9950. | 2014 | 25183333 |
| fast and continuous microorganism detection using aptamer-conjugated fluorescent nanoparticles on an optofluidic platform. | fast and accurate pathogen detection in aquatic environments is challenging in many biomedical studies and microbial diagnostic applications. in this study, we developed a real-time, continuous, and non-destructive single cell detection method using target specific aptamer-conjugated fluorescent nanoparticles (a-fnps) and an optofluidic particle-sensor platform. a-fnps selectively bound to the surfaces of target bacteria (escherichia coli) and labeled them with high affinity and selectivity so t ... | 2015 | 25190089 |
| fabrication of swcnt-ag nanoparticle hybrid included self-assemblies for antibacterial applications. | the present article reports the development of soft nanohybrids comprising of single walled carbon nanotube (swcnt) included silver nanoparticles (agnps) having superior antibacterial property. in this regard aqueous dispersing agent of carbon nanotube (cnt) containing a silver ion reducing unit was synthesised by the inclusion of tryptophan and tyrosine within the backbone of the amphiphile. the dispersions were characterized spectroscopically and microscopically using tem, afm and raman spectr ... | 2014 | 25191756 |
| phenotypic and molecular characteristics of carbapenem-resistant enterobacteriaceae in a health care system in los angeles, california, from 2011 to 2013. | carbapenem-resistant enterobacteriaceae (cre) are a concern for health care in the united states but remain relatively uncommon in california. we describe the phenotype, clonality, and carbapenemase-encoding genes present in cre isolated from patients at a californian tertiary health care system. cre for this study were identified by evaluating the antibiograms of enterobacteriaceae isolated in the ucla health system from 2011 to 2013 for isolates that were not susceptible to meropenem and/or im ... | 2014 | 25210072 |
| siderophore-mediated iron acquisition influences motility and is required for full virulence of the xylem-dwelling bacterial phytopathogen pantoea stewartii subsp. stewartii. | iron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form. pantoea stewartii subsp. stewartii, the causal agent of stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. the proteins involved in the biosynthesi ... | 2014 | 25326304 |
| siderophore-mediated iron acquisition influences motility and is required for full virulence of the xylem-dwelling bacterial phytopathogen pantoea stewartii subsp. stewartii. | iron is a key micronutrient for microbial growth but is often present in low concentrations or in biologically unavailable forms. many microorganisms overcome this challenge by producing siderophores, which are ferric-iron chelating compounds that enable the solubilization and acquisition of iron in a bioactive form. pantoea stewartii subsp. stewartii, the causal agent of stewart's wilt of sweet corn, produces a siderophore under iron-limiting conditions. the proteins involved in the biosynthesi ... | 2014 | 25326304 |
| draft genome assemblies of enterobacter aerogenes cdc 6003-71, enterobacter cloacae cdc 442-68, and pantoea agglomerans ua 0804-01. | the enterobacteriaceae are environmental and enteric microbes. we sequenced the genomes of two enterobacter reference strains, e. aerogenes cdc 6003-71 and e. cloacae cdc 442-68, as well as one near neighbor used as an exclusionary reference for diagnostics, pantoea agglomerans cdc ua0804-01. the genome sizes range from 4.72 to 5.55 mbp and have g+c contents from 54.6 to 55.1%. | 2014 | 25342683 |
| in response to: "bhat ss, undrakonda v, mukhopadhyay c, parmar pv. outbreak of multidrug-resistant acute postoperative endophthalmitis due to enterobacter aerogenes.". | 2015 | 25356796 | |
| authors reply to letter to the editor--in response to: "bhat ss, undrakonda v, mukhopadhyay c, parmar pv. outbreak of multidrug-resistant acute postoperative endophthalmitis due to enterobacter aerogenes". | 2015 | 25356851 | |
| first report of a clinical, multidrug-resistant enterobacteriaceae isolate coharboring fosfomycin resistance gene fosa3 and carbapenemase gene blakpc-2 on the same transposon, tn1721. | in order to understand the genetic background and dissemination mechanism of carbapenem resistance and fosfomycin resistance in enterobacteriaceae isolates, we studied a clinical escherichia coli strain hs102707 isolate and an enterobacter aerogenes strain hs112625 isolate, both of which were resistant to carbapenem and fosfomycin and positive for the bla(kpc-2) and fosa3 genes. in addition, a clinical klebsiella pneumoniae strain hs092839 isolate which was resistant to carbapenem was also studi ... | 2015 | 25367902 |
| the preliminary assessment of anti-microbial activity of hplc separated components of kirkia wilmsii. | most communities in developing countries rely on traditional medicines for the treatment of diseases. in south africa, the limpopo province, within the lebowakgomo district, uses tuberous roots of kirkia wilmsii, after infusion in water for the treatment of a wide range of diseases by sotho communities. | 2014 | 25371593 |
| infected hydatid cysts bacteria in slaughtered livestock and their effects on protoscoleces degeneration. | the protoscoleces of fertile hydatid cysts are considered as major risks in surgery and producing secondary cysts if rupture the cyst during operation and, cause infecting the dogs with adult worm if eaten by this animal. bacterial infection of the hydatid fluid can lead to sterilization of the cyst. | 2014 | 25371792 |
| effects of eliminating pyruvate node pathways and of coexpression of heterogeneous carboxylation enzymes on succinate production by enterobacter aerogenes. | lowering the ph in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs. newly isolated enterobacter aerogenes strain aj110637, a rapid carbon source assimilator under weakly acidic (ph 5.0) conditions, was selected as a platform for succinate production. our previous work showed that the δadhe/pck strain, developed from aj110637 with inactivated ethanol dehydrogenase and introduced actinobacillus succinogenes phosphoenolpyruvate carboxykinase ... | 2015 | 25416770 |
| cytotoxic, antibacterial and antioxidant activities of extracts of the bark of melia azedarach (china berry). | nature provides a variety of drugs and medicinal agents derived from plants. this study was conducted to determine antimicrobial, antioxidant and cytotoxic activities of extracts of melia azedarach bark with methanol/water (9:1 v/v), chloroform, butanol, hexane, water and ethyl acetate. for the determination of the antimicrobial activities, the agar well diffusion method was employed. cytotoxicity was studied by brine shrimp lethality assay; antioxidant activities were measured using 1,1-dipheny ... | 2015 | 25426766 |
| purification of a secreted lectin from andrias davidianus skin and its antibacterial activity. | a lectin secreted from andrias davidianus skin (adl) was purified by affinity chromatography on porcine stomach mucin (type iii) (psm)-crosslinked albumin, followed by gel filtration on sephadex g-100 and hplc on tsk gel g3000pwxl. the purified lectin was found to be a dimeric protein, as revealed by sds-page and maldi-tof analysis. sds-page showed that the adl protein had a molecular mass of 17 kda. adl produced an 8.5 kda band when examined using sds-page under reducing conditions. adl aggluti ... | 2015 | 25445018 |
| use of aiia gene amplification for ahl-lactonase production from endophytic bacterium enterobacter species. | ahl-lactonase has gained renewed interest due to biotechnological applications such as antiquorum sensing, antibiofilm strategies, biofouling, etc. in our study, the production of ahl-lactonase from endophytic bacteria enterobacter aerogenes vt66 was optimized by response surface methodology (rsm) using central composite design (ccd) for four different cultural conditions. the relative activity of ahl-lactonase was correlated with amplification of aiia homologous gene amplification with respect ... | 2015 | 25451751 |
| combining urban wastewater treatment with biohydrogen production--an integrated microalgae-based approach. | the aim of the present work was the simultaneous treatment of urban wastewater using microalgae and the energetic valorization of the obtained biomass. chlorella vulgaris (cv), scenedesmus obliquus (sc) and a naturally occurring algal consortium c (consc) were grown in an urban wastewater. the nutrient removals were quite high and the treated water fits the legislation (pt dec-lei 236/98) in what concerns the parameters analysed (n, p, cod). after nutrient depletion the microalgae remained two m ... | 2015 | 25453433 |
| egcg assisted green synthesis of zno nanopowders: photodegradative, antimicrobial and antioxidant activities. | zinc oxide nanopowders were synthesized by solution combustion method using epigallocatechin gallate (egcg) a tea catechin as fuel. the structure and morphology of the product was characterized by powder x-ray diffraction, scanning electron microscopy, photoluminescence and uv-visible spectroscopy. the nanopowders (nps) were subjected to photocatalytic and biological activities such as antimicrobial and antioxidant studies. pxrd patterns demonstrate that the formed product belongs to hexagonal w ... | 2015 | 25459708 |
| restrictive reporting of selected antimicrobial susceptibilities influences clinical prescribing. | cascade and restrictive reporting are useful strategies to enhance antibiotic stewardship programs. | 2016 | 25466592 |
| effect of white mustard essential oil on the growth of foodborne pathogens and spoilage microorganisms and the effect of food components on its efficacy. | antimicrobial preservative compounds are added to foods to target specific pathogens and spoilage organisms. white mustard essential oil (wmeo) is an extract that contains 4-hydroxybenzyl isothiocyanate, a compound which has been demonstrated to have antimicrobial activity in limited studies. the objective of this research was to determine the in vitro antimicrobial activity of wmeo against gram-positive and gram-negative spoilage and pathogenic bacteria and determine the effect of food componen ... | 2014 | 25474051 |
| antioxidant and antibacterial potential of pomegranate peel extracts. | pomegranate peels of ganesh variety were subjected to extraction using different solvents viz. water, methanol and ethanol either alone or in combination with water. the extraction yield, antioxidant activity (dpph and abts inhibition) and total phenolic contents were evaluated. highest yield was obtained from 50 % ethanol: 50 % water (16.3 ± 1.99 %). the dpph and abts inhibition activity was found to be the highest for methanol and 70 % ethanol: 30 % water extract (79.5 ± 6.5; 94.6 ± 6.10), res ... | 2014 | 25477693 |
| production of humic substances through coal-solubilizing bacteria. | in this paper, the production of humic substances (hs) through the bacterial solubilization of low rank coal (lrc) was evaluated. the evaluation was carried out by 19 bacterial strains isolated in microenvironments with high contents of coal wastes. the biotransformed lrc and the hs produced were quantified in vitro in a liquid growth medium. the humic acids (ha) obtained from the most active bacterial strain were characterized via elemental composition (c, h, n, o), ir analyses, and the e4/e6 r ... | 2014 | 25477925 |
| screening of wild type streptomyces isolates able to overproduce clavulanic acid. | the selection of new microorganisms able to produce antimicrobial compounds is hoped for to reduce their production costs and the side effects caused by synthetic drugs. clavulanic acid is a β-lactam antibiotic produced by submerged culture, which is widely used in medicine as a powerful inhibitor of β-lactamases, enzymes produced by bacteria resistant to antibiotics such penicillin and cephalosporin. the purpose of this work was to select the best clavulanic acid producer among strains of strep ... | 2014 | 25477926 |
| activity of eravacycline against enterobacteriaceae and acinetobacter baumannii, including multidrug-resistant isolates, from new york city. | eravacycline demonstrated in vitro activity against a contemporary collection of more than 4,000 gram-negative pathogens from new york city hospitals, with mic50/mic90 values, respectively, for escherichia coli of 0.12/0.5 μg/ml, klebsiella pneumoniae of 0.25/1 μg/ml, enterobacter aerogenes of 0.25/1 μg/ml, enterobacter cloacae 0.5/1 μg/ml, and acinetobacter baumannii of 0.5/1 μg/ml. activity was retained against multidrug-resistant isolates, including those expressing kpc and oxa carbapenemases ... | 2015 | 25534744 |
| polygalacturonase production by calcium alginate immobilized enterobacter aerogenes nbo2 cells. | bacterial cells of enterobacter aerogenes nbo2 were entrapped in calcium alginate beads in order to enhance polygalacturonase production compared to free cells. the optimized condition of 5 % (w/v) sodium alginate concentration, agitation speed of 250 rpm, and 15 beads of calcium alginate with inoculum size of 4 % (v/v; 5.4 × 10(7) cells/ml) produced 23.48 u/ml of polygalacturonase compared to free cells of 18.54 u/ml. there was about 26.6 % increment in polygalaturonase production. however, in ... | 2015 | 25547814 |
| four carbapenem-resistant gram-negative species carrying distinct carbapenemases in a single patient. | carbapenem-resistant escherichia coli, klebsiella pneumoniae, enterobacter aerogenes, and acinetobacter baumannii were isolated from a single patient, each producing different carbapenemases (ndm-1, kpc-2, imp, and oxa-23, respectively). the ndm-1-producing e. coli strain was preceded by a clonally related carbapenem-susceptible strain a month earlier, suggesting in vivo acquisition of blandm-1. | 2015 | 25552359 |
| structure of n-formimino-l-glutamate iminohydrolase from pseudomonas aeruginosa. | n-formimino-l-glutamate iminohydrolase (hutf), from pseudomonas aeruginosa with a locus tag of pa5106 ( gi|15600299 ), is a member of the amidohydrolase superfamily. this enzyme catalyzes the deamination of n-formimino-l-glutamate to n-formyl-l-glutamate and ammonia in the histidine degradation pathway. the crystal structure of pa5106 was determined in the presence of the inhibitors n-formimino-l-aspartate and n-guanidino-l-glutaric acid at resolutions of 1.9 and 1.4 å, respectively. the structu ... | 2015 | 25559274 |
| chemical composition and antimicrobial evaluation of the essential oils of bocageopsis pleiosperma maas. | essential oils from the leaves, twigs and barks of bocageopsis pleiosperma maas were obtained by using hydrodistillation and analysed by using gas chromatography coupled to mass spectrometry. several compounds (51) were detected and identified, being β-bisabolene the main component in all aerial parts of the plant, with higher concentration in the leaves (55.77%), followed by barks (38.53%) and twigs (34.37%). in order to increase the biological knowledge about the essential oil of bocageopsis s ... | 2015 | 25562370 |
| a new antimicrobial and radical-scavenging glycoside from paullinia pinnata var. cameroonensis. | a new glycoside, pinnatoside a (1), together with two known compounds (2 and 3), were isolated from the stems of paullinia pinnata. their structures were elucidated on the basis of extensive spectroscopic analysis and chemical methods. compound 1 showed significant antibacterial activity with a minimum inhibitory concentration (mic) value of 1.56 μg/ml against escherichia coli, and 2 displayed significant antibacterial activity with a mic value of 1.56 μg/ml against enterobacter aerogenes and e. ... | 2015 | 25563339 |
| amym, a novel maltohexaose-forming α-amylase from corallococcus sp. strain egb. | a novel α-amylase, amym, was purified from the culture supernatant of corallococcus sp. strain egb. amym is a maltohexaose-forming exoamylase with an apparent molecular mass of 43 kda. based on the results of matrix-assisted laser desorption ionization-time of flight mass spectrometry and peptide mass fingerprinting of amym and by comparison to the genome sequence of corallococcus coralloides dsm 2259, the amym gene was identified and cloned into escherichia coli. amym encodes a secretory amylas ... | 2015 | 25576603 |
| reduction of urease activity by interaction with the flap covering the active site. | with the increasing appreciation for the human microbiome coupled with the global rise of antibiotic resistant organisms, it is imperative that new methods be developed to specifically target pathogens. to that end, a novel computational approach was devised to identify compounds that reduce the activity of urease, a medically important enzyme of helicobacter pylori, proteus mirabilis, and many other microorganisms. urease contains a flexible loop that covers its active site; glide was used to i ... | 2015 | 25594724 |
| antibacterial efficacy of five medicinal plants against multidrug-resistant enteropathogenic bacteria infecting under-5 hospitalized children. | to evaluate in vitro antibacterial effectiveness of five medicinal plants used by an indian aborigine, against 8 multidrug-resistant (mdr) enteropathogenic bacteria isolated from clinical samples of under-5 hospitalized children. | 2015 | 25609371 |
| a sequential statistical approach towards an optimized production of a broad spectrum bacteriocin substance from a soil bacterium bacillus sp. yas 1 strain. | bacteriocins, ribosomally synthesized antimicrobial peptides, display potential applications in agriculture, medicine, and industry. the present study highlights integral statistical optimization and partial characterization of a bacteriocin substance from a soil bacterium taxonomically affiliated as bacillus sp. yas 1 after biochemical and molecular identifications. a sequential statistical approach (plackett-burman and box-behnken) was employed to optimize bacteriocin (bac yas 1) production. u ... | 2014 | 25614886 |
| prevalence and antibiotic resistance of gram-negative pathogenic bacteria species isolated from periplaneta americana and blattella germanica in varanasi, india. | cockroaches are among the medically important pests found within the human habitations that cause serious public health problems. they may harbor a number of pathogenic bacteria on the external surface with antibiotic resistance. hence, they are regarded as major microbial vectors. this study investigates the prevalence and antibiotic resistance of gram-negative pathogenic bacteria species isolated from periplaneta americana and blattella germanica in varanasi, india. | 2013 | 25629061 |
| aerobic microbial community of insectary population of phlebotomus papatasi. | microbes particularly bacteria presenting in the gut of haematophagous insects may have an important role in the epidemiology of human infectious disease. | 2013 | 25629067 |
| aerobic microbial community of insectary population of phlebotomus papatasi. | microbes particularly bacteria presenting in the gut of haematophagous insects may have an important role in the epidemiology of human infectious disease. | 2013 | 25629067 |
| association of tellurite resistance with hypervirulent clonal groups of klebsiella pneumoniae. | tellurite-based selective growth media are used for several bacterial pathogens. we found that, in klebsiella pneumoniae, tellurite resistance is strongly associated with hypervirulent clonal group 23 (cg23), cg65, and cg86, providing a novel approach for screening environmental or carriage samples. the terw gene was also associated with these groups. | 2015 | 25631812 |
| probiotic potential of lactobacillus strains isolated from sorghum-based traditional fermented food. | sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. the isolates were identified by biochemical, physiological and genetic methods. species identification was done by 16s rrna sequence analysis. the functional probiotic potential of the two lactobacillus species viz., lactobacillus plantarum (lact. plantarum) and lactobacillus pentosus (lact. pentosus) was assessed by different standard parameters. the strains were tolerant to ph 2 for 1 h and resi ... | 2015 | 25666113 |
| safety, efficacy and utility of methods of transferring adhesive and cohesive escherichia coli cells to microplates to avoid aerosols. | the virtual colony count (vcc) microbiological assay has been utilized for over a decade to measure the antimicrobial activity of peptides such as defensins and ll-37 against biosafety level (bsl)-1 and bsl-2 bacteria including escherichia coli, staphylococcus aureus, bacillus cereus, and enterobacter aerogenes. in addition, a modified pipetting technique was presented in a 2011 study of defensin activity against the bsl-3 pathogen bacillus anthracis. both studies were published in the journal ... | 2014 | 25671086 |
| pyrosequencing analysis of microbial community and food-borne bacteria on restaurant cutting boards collected in seri kembangan, malaysia, and their correlation with grades of food premises. | this study adopts the pyrosequencing technique to identify bacteria present on 26 kitchen cutting boards collected from different grades of food premises around seri kembangan, a city in malaysia. pyrosequencing generated 452,401 of total reads of otus with an average of 1.4×10(7) bacterial cells/cm(2). proteobacteria, firmicutes and bacteroides were identified as the most abundant phyla in the samples. taxonomic richness was generally high with >1000 operational taxonomic units (otus) observed ... | 2015 | 25679309 |
| spectral and biological evaluation of a synthetic antimicrobial peptide derived from 1-aminocyclohexane carboxylic acid. | ac-gf(a6c)g(a6c)k(a6c)g(a6c)f(a6c)g(a6c)gk(a6c)kkkk-amide (a6c=1-aminocyclohexane carboxylic acid) is a synthetic antimicrobial peptide (amp) that exhibits in vitro inhibitory activity against drug resistant strains of staphylococcus aureus, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa, enterobacter aerogenes, and enterococcus faecium at concentrations ranging from 10.9 to 43μm. spectroscopic investigations were conducted to determine how this amp interacts with simple ... | 2015 | 25684423 |
| engineering tobacco to remove mercury from polluted soil. | tobacco is an ideal plant for modification to remove mercury from soil. although several transgenic tobacco strains have been developed, they either release elemental mercury directly into the air or are only capable of accumulating small quantities of mercury. in this study, we constructed two transgenic tobacco lines: ntk-7 (a tobacco plant transformed with mert-merp-merb1-merb2-ppk) and ntp-36 (tobacco transformed with mert-merp-merb1-merb2-pcs1). the genes mert, merp, merb1, and merb2 were o ... | 2015 | 25690353 |
| identification and inhibition of histamine-forming bacteria in blue scad (decapterus maruadsi) and chub mackerel (scomber japonicus). | in this study, we investigated the differences in histamine accumulation between blue scad and chub mackerel and methods of inhibiting histamine-forming bacteria and controlling histamine accumulation in fish. the free histidine contents in blue scad and chub mackerel were 1.45 and 2.75 mg/g, respectively. the histamine-forming bacteria isolated from them were identified as citrobacter freundii, citrobacter braakii, and enterobacter aerogenes using 16s rdna sequence analysis, the vitek 2 compact ... | 2015 | 25710155 |
| kpc-producing enterobacter aerogenes infection. | enterobacter is a common nosocomial microorganism and its carbapenem's resistance has increased. the management of these cases is unclear. | 2015 | 25722130 |
| extended-spectrum-β-lactamase-producing enterobacteriaceae isolated from vegetables imported from the dominican republic, india, thailand, and vietnam. | to examine to what extent fresh vegetables imported into switzerland represent carriers of extended-spectrum-β-lactamase (esbl)-producing enterobacteriaceae, 169 samples of different types of fresh vegetables imported into switzerland from the dominican republic, india, thailand, and vietnam were analyzed. overall, 25.4% of the vegetable samples yielded one or more esbl-producing enterobacteriaceae, 78.3% of which were multidrug resistant. sixty isolates were obtained: escherichia coli, 26; kleb ... | 2015 | 25724954 |
| common isolation of new delhi metallo-beta-lactamase 1-producing enterobacteriaceae in a large surgical hospital in vietnam. | this study sought to monitor the presence of carbapenem-resistant enterobacteriaceae (cre) and the proportion new delhi metallo-beta-lactamase 1 (ndm-1)-producing bacteria between august 2010 and december 2012 in a surgical hospital in vietnam. we identified 47 cre strains from a total of 4,096 enterobacteriaceae isolates (1.1 %) that were ndm-1-positive from 45 patients admitted to 11 different departments, with the majority being from the urology department. the ndm-1 gene was found in seven d ... | 2015 | 25732142 |
| directed evolution: past, present and future. | directed evolution, the laboratory process by which biological entities with desired traits are created through iterative rounds of genetic diversification and library screening or selection, has become one of the most useful and widespread tools in basic and applied biology. from its roots in classical strain engineering and adaptive evolution, modern directed evolution came of age twenty years ago with the demonstration of repeated rounds of pcr-driven random mutagenesis and activity screening ... | 2013 | 25733775 |
| in vitro activity of ceftazidime, ceftaroline and aztreonam alone and in combination with avibactam against european gram-negative and gram-positive clinical isolates. | recent clinical isolates of key gram-negative and gram-positive bacteria were collected in 2012 from hospitalised patients in medical centres in four european countries (france, germany, italy and spain) and were tested using standard broth microdilution methodology to assess the impact of 4 mg/l avibactam on the in vitro activities of ceftazidime, ceftaroline and aztreonam. against enterobacteriaceae, addition of avibactam significantly enhanced the level of activity of these antimicrobials. mi ... | 2015 | 25748553 |
| how fast-growing bacteria robustly tune their ribosome concentration to approximate growth-rate maximization. | maximization of growth rate is an important fitness strategy for bacteria. bacteria can achieve this by expressing proteins at optimal concentrations, such that resources are not wasted. this is exemplified for escherichia coli by the increase of its ribosomal protein-fraction with growth rate, which precisely matches the increased protein synthesis demand. these findings and others have led to the hypothesis that e. coli aims to maximize its growth rate in environments that support growth. howe ... | 2015 | 25754869 |
| predominance of kpc-3 in a survey for carbapenemase-producing enterobacteriaceae in portugal. | among the 2,105 enterobacteriaceae tested in a survey done in portugal, 165 were nonsusceptible to carbapenems, from which 35 (26 klebsiella pneumoniae, 3 escherichia coli, 2 enterobacter aerogenes, and 3 enterobacter cloacae isolates and 1 klebsiella oxytoca isolate) were confirmed to be carbapenemase producers by the presence of 30 tn4401d-blakpc-3, 4 inti3-blages-5, and one inti1-blavim-2 gene, alone or in combination with other bla genes. the dissemination of blakpc-3 gene carried by an incf ... | 2015 | 25779587 |
| separation and confirmation of nine enterobacteriaceae strains that carry the blandm-1 gene. | the aim of the present study was to confirm the existence of carbapenem-resistant enterobacteriaceae carrying the blandm-1 gene in clinics in hainan province, china. collected clinical bacterial isolates that were enterobacteriaceae strains suspected of producing carbapenemase were used as experimental strains. drug resistance to imipenem, meropenem and other antibacterial agents was tested. imipenem/imipenem inhibitor (ip/ipi) e-testing was conducted to identify the bacterial strains that produ ... | 2015 | 25780416 |
| transcriptional regulation, metal binding properties and structure of pden1597, an unusual zinc transport protein from paracoccus denitrificans. | atp-binding cassette (abc) transporters of the cluster 9 family are ubiquitous among bacteria and essential for acquiring zn(2+) and mn(2+) from the environment or, in the case of pathogens, from the host. these rely on a substrate-binding protein (sbp) to coordinate the relevant metal with high affinity and specificity and subsequently release it to a membrane permease for translocation into the cytoplasm. although a number of cluster 9 sbp structures have been determined, the structural attrib ... | 2015 | 25787075 |
| 2-alkyl(aryl)-quinazolin-4(3h)-thiones, 2-r-(quinazolin-4(3h)-ylthio)carboxylic acids and amides: synthesis, molecular docking, antimicrobial and anticancer properties. | in this study, a series of novel 2-alkyl(aryl)-quinazolin-4(3h)-thiones, 2-r-(quinazolin-4(3h)-ylthio)carboxylic acids and amides were synthesized and evaluated for antimicrobial and anticancer activities. their structure was confirmed by elemental analysis and spectral data (ft-ir, lc-ms, (1)h-nmr). antimicrobial activity was tested in vitro against staphylococcus aureus, enterococcus faecalis, enterobacter aerogenes, pseudomonas aeruginosa, escherichia coli, klebsiella pneumonia, candida albic ... | 2016 | 25831026 |
| quantifying the effect of hand wash duration, soap use, ground beef debris, and drying methods on the removal of enterobacter aerogenes on hands. | hand washing is recognized as a crucial step in preventing foodborne disease transmission by mitigating crosscontamination among hands, surfaces, and foods. this research was undertaken to establish the importance of several keys factors (soap, soil, time, and drying method) in reducing microorganisms during hand washing. a nonpathogenic nalidixic acid-resistant enterobacter aerogenes surrogate for salmonella was used to assess the efficacy of using soap or no soap for 5 or 20 s on hands with or ... | 2015 | 25836392 |
| an efficient ribitol-specific dehydrogenase from enterobacter aerogenes. | an nad(+)-dependent ribitol dehydrogenase from enterobacter aerogenes kctc 2190 (eardh) was cloned and successfully expressed in escherichia coli. the complete 729-bp gene was amplified, cloned, expressed, and subsequently purified in an active soluble form using nickel affinity chromatography. the enzyme had an optimal ph and temperature of 11.0 and 45°c, respectively. among various polyols, eardh exhibited activity only toward ribitol, with km, vmax, and kcat/km values of 10.3mm, 185umg(-1), a ... | 2015 | 25837508 |
| comparison of validated polymerase chain reaction and culture isolation for the routine detection of acanthamoeba from ocular samples. | acanthamoeba keratitis should be definitively diagnosed for appropriate therapy. our institution has validated polymerase chain reaction (pcr) as a routine diagnostic test to detect acanthamoeba dna from ocular samples. we compared pcr with culture isolation for detecting acanthamoeba from ocular samples. | 2015 | 25839345 |
| draft genome sequence of enterobacter aerogenes, a dde-degrading and plant growth-promoting strain isolated from cucurbita pepo. | we report here the draft genome of enterobacter aerogenes, a gram-negative bacterium of the enterobacteriaceae isolated from cucurbita pepo root tissue. this bacterium shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (dde)-degrading potential and plant growth-promoting capacity. an analysis of its 4.5-mb draft genome will enhance the understanding of dde degradation pathways and phytoremediation applications for dde-contaminated soils. | 2015 | 25883299 |
| repurposing salicylanilide anthelmintic drugs to combat drug resistant staphylococcus aureus. | staphylococcus aureus is a gram-positive bacterium that has become the leading cause of hospital acquired infections in the us. repurposing food and drug administration (fda) approved drugs for antimicrobial therapy involves lower risks and costs compared to de novo development of novel antimicrobial agents. in this study, we examined the antimicrobial properties of two commercially available anthelmintic drugs. the fda approved drug niclosamide and the veterinary drug oxyclozanide displayed str ... | 2015 | 25897961 |
| casque infection, resolution, and subsequent repneumatization in a trumpeter hornbill (bycanistes buccinator). | 2015 | 25909990 | |
| bacterial community structure in treated sewage sludge with mesophilic and thermophilic anaerobic digestion. | stabilized sewage sludge is applied to agricultural fields and farmland due to its high organic matter content. the aim of this study was to investigate the effects of two types of sludge stabilization, mesophilic anaerobic digestion (mad) and thermophilic anaerobic digestion (tad), on bacterial communities in sludge, including the presence of pathogenic microorganisms. bacterial community structure and phylogenetic diversity were analyzed in four sewage sludge samples from the czech republic. a ... | 2015 | 25921720 |
| ndm-1 encoded by a pndm-bj01-like plasmid p3sp-ndm in clinical enterobacter aerogenes. | a carbapenem-nonsusceptible enterobacter aerogenes strain named 3-sp was isolated from a human case of pneumonia in a chinese teaching hospital. ndm-1 carbapenemase is produced by a pndm-bj01-like conjugative plasmid designated p3sp-ndm to account for carbapenem resistance of 3-sp. p3sp-ndm was fully sequenced and compared with all publically available pndm-bj01-like plasmids. the genetic differences between p3sp-ndm and pndm-bj01 include only 18 single nucleotide polymorphisms, a 1 bp deletion ... | 2015 | 25926823 |
| further modification of the modified hodge test for detecting metallo-β-lactamase-producing carbapenem-resistant enterobacteriaceae. | the modified hodge test (mht) was designed to detect carbapenemase-producing enterobacteriaceae (cpe). this study evaluated variables to improve the performance of mht. | 2015 | 25932437 |
| molecular epidemiology of kpc-2-producing enterobacteriaceae (non-klebsiella pneumoniae) isolated from brazil. | in brazil, since 2009, there has been an ever increasing widespread of the bla(kpc-2) gene, mainly in klebsiella pneumoniae. this study aims to assess the molecular epidemiology and genetic background of this gene in enterobacteriaceae (non-k. pneumoniae) species from 9 brazilian states between 2009 and 2011. three hundred eighty-seven isolates were analyzed exhibiting nonsusceptibility to carbapenems, in which the bla(kpc-2) gene was detected in 21.4%. by disk diffusion and e-test, these isolat ... | 2015 | 25935630 |
| biohydrogen and bioethanol production from biodiesel-based glycerol by enterobacter aerogenes in a continuous stir tank reactor. | crude glycerol from the biodiesel manufacturing process is being produced in increasing quantities due to the expanding number of biodiesel plants. it has been previously shown that, in batch mode, semi-anaerobic fermentation of crude glycerol by enterobacter aerogenes can produce biohydrogen and bioethanol simultaneously. the present study demonstrated the possible scaling-up of this process from small batches performed in small bottles to a 3.6-l continuous stir tank reactor (cstr). fresh feed ... | 2015 | 25970750 |
| clonal dissemination of oxa-370-producing klebsiella pneumoniae in rio de janeiro, brazil. | enzymes of the oxa-48 family have become some of the most important beta-lactamases in the world. a new oxa-48 variant (oxa-370) was first described for an enterobacter hormaechei strain isolated in rio grande do sul (southern region of brazil) in 2013. here we report detection of the blaoxa-370 gene in 24 isolates belonging to three enterobacteriaceae species (22 klebsiella pneumoniae isolates, 1 enterobacter cloacae isolate, and 1 enterobacter aerogenes isolate) collected from five hospitals i ... | 2015 | 25987619 |
| antimicrobial, antitumor and brine shrimp lethality assay of ranunculus arvensis l. extracts. | to investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of methanol extract (me), water extract (we), acetone extract (ae), chloroform extract (ce), methanol-water extract (mwe), methanol-acetone extract (mae), methanol-chloroform extract (mce) of ranunculus arvensis (l.). antitumor activity was evaluated with agrobacterium tumefaciens (at10) induced potato disc assay. cytotoxicity was evaluated with brine shrimp lethality assay. antibacterial a ... | 2015 | 26004705 |
| [emphysematous cystitis: report of one case]. | emphysematous cystitis is found in diabetic patients and in individuals with urinary stasis and immunosuppression. we report a 58-year-old male with hypertension, type 2 diabetes on insulin treatment and central nervous system vasculitis on immunosuppressive therapy. he was admitted with weight loss and gait instability. a pet-ct showed a circumscribed image of air in the bladder contour without involving the upper urinary tract, suggesting emphysematous cystitis. re-interrogated, the patient re ... | 2015 | 26005827 |
| proteolytic and antimicrobial activity of lactic acid bacteria grown in goat milk. | we examined 62 strains and 21 trade starter cultures from the collection of lb bulgaricum plc for proteolytic and antimicrobial activity of lactic acid bacteria (lab) grown in goat milk. the aim of this study was to investigate the fermentation of caseins, α-lactalbumin and β-lactoglobulin by lab, using the o-phthaldialdehyde (opa) spectrophotometric assay and sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). the proteolysis targeted mainly caseins, especially β-casein. whey ... | 2014 | 26019593 |
| molecular characteristics of extended-spectrum cephalosporin-resistant enterobacteriaceae from humans in the community. | to investigate the molecular characteristics of extended-spectrum cephalosporin (esc)-resistant enterobacteriaceae collected during a cross-sectional study examining the prevalence and risk factors for faecal carriage of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in humans living in areas with high or low broiler density. | 2015 | 26029910 |
| antimicrobial and cytotoxic activities from jatropha dioica roots. | the antimicrobial and cytotoxic activities of organic extracts obtained from roots of the medicinal plant jatropha dioica (euphorbiaceae) were investigated. in order to evaluate their antimicrobial activity, the organic extracts were tested against clinical isolates of the human pathogens bacillus cereus, escherichia coli, salmonella typhi, staphylococcus aureus, enterobacter aerogenes, enterobacter cloacae, salmonella typhimurium, cryptococcus neoformans, candida albicans, candida parapsilosis ... | 2014 | 26031013 |
| identification and characterization of a novel incompatibility group x3 plasmid carrying bla ndm-1 in enterobacteriaceae isolates with epidemiological links to multiple geographical areas in china. | the new delhi metallo-β-lactamase (ndm-1) is one of the most important resistance traits in enterobacteriaceae. we characterized nine bla ndm-1 producing enterobacteriaceae recovered from seven patients who have recently travelled or been treated in india (n=1) or mainland china (n=6) during december 2010-may 2012. all the china-linked patients had no links to the indian subcontinent. the bla ndm-1 carrying plasmids belonged to the novel incx3 (∼50 kb, in seven isolates including two escherichia ... | 2012 | 26038408 |
| enterobacter aerogenes and enterobacter cloacae; versatile bacterial pathogens confronting antibiotic treatment. | enterobacter aerogenes and e. cloacae have been reported as important opportunistic and multiresistant bacterial pathogens for humans during the last three decades in hospital wards. these gram-negative bacteria have been largely described during several outbreaks of hospital-acquired infections in europe and particularly in france. the dissemination of enterobacter sp. is associated with the presence of redundant regulatory cascades that efficiently control the membrane permeability ensuring th ... | 2015 | 26042091 |
| activities of selected medicinal plants against multi-drug resistant gram-negative bacteria in cameroon. | medicinal plants are used worldwide for several human ailments including bacterial infections. the present work was designed to assess the in vitro antibacterial activities of some cameroonian medicinal plants including entada abyssinica, entada africana, pentaclethra macrophylla, allexis cauliflora, anthocleista leibrechtsiana, carapa procera, carica papaya and persea americana against gram-negative bacteria expressing multidrug resistant (mdr) phenotypes. | 2014 | 26060474 |
| impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in enterobacter aerogenes. | succinate is an important c4 building block chemical, and its production via fermentative processes in bacteria has many practical applications in the biotechnology field. one of the major goals of optimizing the bacterium-based succinate production process is to lower the culture ph from the current neutral conditions, as this would reduce total production costs. in our previous studies, we selected enterobacter aerogenes, a rapid glucose assimilator at ph 5.0, in order to construct a metabolic ... | 2015 | 26063229 |
| toxicity of chlortetracycline and its metal complexes to model microorganisms in wastewater sludge. | complexation of antibiotics with metals is a well-known phenomenon. wastewater treatment plants contain metals and antibiotics, thus it is essential to know the effect of these complexes on toxicity towards microorganisms, typically present in secondary treatment processes. in this study, stability constants and toxicity of chlortetracycline (ctc) and metal (ca, mg, cu and cr) complexes were investigated. the calculated stability constants of ctc-metal complexes followed the order: mg-ctc>ca-ctc ... | 2015 | 26119381 |
| antibacterial activity of combination of synthetic and biopolymer non-woven structures. | fibrous structures and synthetic polymer blends offer potential usages in making biomedical devices, textiles used in medical practices, food packaging, tissue engineering, environmental applications and biomedical arena. these products are also excellent candidates for building scaffolds to grow stem cells for implantation, to make tissue engineering grafts, to make stents to open up blood vessels caused by atherosclerosis or narrowed by blood clots, for drug delivery systems for micro- to nano ... | 2015 | 26124061 |
| distribution and gene mutation of enteric flora carrying β-glucuronidase among patients with colorectal cancer. | to explore the difference of distribution in intestinal flora among colorectal cancer patients and healthy controls and investigate characteristics and changes of sequences in beta-glucuronidase (β-glucuronidase, β-g). | 2015 | 26131106 |
| biological activities of morus celtidifolia leaf extracts. | the aims of this research were to examine the antibacterial, cytotoxic and antiradical/antioxidant activities of the organic extracts obtained from the leaves of the medicinal plant morus celtidifolia (family: moraceae). to evaluate its antimicrobial properties, m. celtidifolia was tested against the bacteria of medical importance: bacillus subtilis, staphyloccocus aureus, enterococcus faecalis, escherichia coli, enterobacter cloacae and enterobacter aerogenes. cytotoxic activity was assessed by ... | 2015 | 26142508 |
| biohydrogen production by co-fermentation of crude glycerol and apple pomace hydrolysate using co-culture of enterobacter aerogenes and clostridium butyricum. | co-substrate utilization of various wastes with complementary characteristics can provide a complete medium for higher hydrogen production. this study evaluated potential of apple pomace hydrolysate (aph) co-fermented with crude glycerol (cg) for increased h2 production and decreased by-products formation. the central composite design (ccd) along with response surface methodology (rsm) was used as tool for optimization and 15 g/l of cg, 5 g/l of aph and 15% (v/v) inoculum were found to be optimu ... | 2015 | 26142996 |
| isolation, development and identification of salt-tolerant bacterial consortium from crude-oil-contaminated soil for degradation of di-azo dye reactive blue 220. | the objective of this study was development and characterization of a halophilic bacterial consortium for rapid decolorization and degradation of a wide range of dyes and their mixtures. the 16s rrna gene analysis of developed halophilic consortium vn.1 showed that the bacterial consortium contained six bacterial strains, which were identified as pseudomonas fluorescens hm480360, enterobacter aerogenes hm480361, shewanella sp. hm589853, arthrobacter nicotianae hm480363, bacillus beijingensis hm4 ... | 2015 | 26177415 |
| disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in enterobacter aerogenes. | hydrogen production by enterobacter aerogenes from glucose was enhanced by deleting the targeted ldha and adh genes responsible for two nadh-consuming pathways which consume most nadh generated from glycolysis. compared with the wild-type, the hydrogen yield of iam1183-δldha increased 1.5 fold. metabolic flux analysis showed both iam1183-δldha and iam1183-δadh exhibited significant changes in flux, including enhanced flux towards the hydrogen generation. the lactate production of iam1183-δldha s ... | 2015 | 26188552 |
| binding to histo-blood group antigen-expressing bacteria protects human norovirus from acute heat stress. | this study aims to investigate if histo-blood group antigen (hbga) expressing bacteria have any protective role on human norovirus (nov) from acute heat stress. eleven bacterial strains were included, belonging to escherichia coli, enterobacter cloacae, enterobacter aerogenes, clostridium difficile, bifidobacterium adolescentis, and b. longum. hbga expression of the bacteria as well as binding of human nov virus-like particles (vlps, gi.1, and gii.4 strains) to the bacteria were detected by flow ... | 2015 | 26191052 |
| prevalence and characterization of high histamine-producing bacteria in gulf of mexico fish species. | recent developments in detection and enumeration of histamine-producing bacteria (hpb) have created powerful molecular-based tools to better understand the presence of spoilage bacteria and conditions, resulting in increased risk of scombrotoxin fish poisoning. we examined 235 scombrotoxin-forming fish from the gulf of mexico for the presence of high hpb. photobacterium damselae subsp. damselae was the most prevalent hpb (49%), followed by morganella morganii (14%), enterobacter aerogenes (4%), ... | 2015 | 26197285 |
| novel glucose-1-phosphatase with high phytase activity and unusual metal ion activation from soil bacterium pantoea sp. strain 3.5.1. | phosphorus is an important macronutrient, but its availability in soil is limited. many soil microorganisms improve the bioavailability of phosphate by releasing it from various organic compounds, including phytate. to investigate the diversity of phytate-hydrolyzing bacteria in soil, we sampled soils of various ecological habitats, including forest, private homesteads, large agricultural complexes, and urban landscapes. bacterial isolate pantoea sp. strain 3.5.1 with the highest level of phytas ... | 2015 | 26209662 |
| alleviation of carbon catabolite repression in enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3-butanediol production. | due to its cost-effectiveness and rich sugar composition, sugarcane molasses is considered to be a promising carbon source for biorefinery. however, the sugar mixture in sugarcane molasses is not consumed as efficiently as glucose in microbial fermentation due to complex interactions among their utilizing pathways, such as carbon catabolite repression (ccr). in this study, 2,3-butanediol-producing enterobacter aerogenes was engineered to alleviate ccr and improve sugar utilization by modulating ... | 2015 | 26236395 |
| facile green fabrication of nanostructure zno plates, bullets, flower, prismatic tip, closed pine cone: their antibacterial, antioxidant, photoluminescent and photocatalytic properties. | green synthesis of multifunctional zinc oxide nanoparticles (nps) with a variety of morphologies were achieved by low temperature solution combustion route employing neem (azadirachta indica) extract as fuel. the nanoparticles were characterized by pxrd, ftir, xps, raman and uv-visible spectroscopic studies. the morphologies were studied by sem and tem analysis. the nps were subjected for photoluminescence, photocatalytic, antibacterial and antioxidant activity studies. pxrd pattern confirmed th ... | 2016 | 26241826 |
| prevalence of bovine subclinical mastitis, its etiology and diagnosis of antibiotic resistance of dairy farms in four municipalities of a tropical region of mexico. | a region-wide survey was conducted in the tropical area of tierra caliente, state of guerrero, mexico to estimate the prevalence of subclinical bovine mastitis (scm), distribution of mastitis pathogens, and in vitro antimicrobial susceptibility of different mastitis pathogens in dairy farms. in total, 1036 quarter milk samples were obtained from 259 cows at 87 different dairy farms. collected quarter milk samples were submitted for california mastitis test (cmt), bacteriological examination, and ... | 2015 | 26255183 |
| synthesis of novel sulfonamide analogs containing sulfamerazine/sulfaguanidine and their biological activities. | sulfamerazine and sulfaguanidine are clenched with p-nitrobenzoyl chloride and the products obtained are reduced to naxs in ethanol-water. novel sulfonamides (6a-g and 9a-g) were synthesized by the reaction of these reduced products (4 and 8) with various sulfonyl chlorides (5a-g). the structures of these compounds were characterized using spectroscopic analysis (ir, (1)h-nmr, (13)c-nmr and hrms) technique. antimicrobial activity of sulfonamides (3, 4, 7, 8, 6a-g and 9a-g) was evaluated by the a ... | 2016 | 26327456 |
| antimicrobial triterpenes from the stem bark of crossopteryx febrifuga. | phytochemical investigation of the stem bark extract of crossopteryx febrifuga resulted in the isolation of epimeric mixtures of 3β-urs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-urs-12,20(30)-diene-27,28-dioic acid (1), as well as: 3β-d-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid and 18-epi-3β-d-glucopyranosylurs-12,20(30)-diene-27,28-dioic acid (2), together with some known compounds such as the monoglyceride of palmitic acid, as well as β-sitosterol and its glucoside. the structure ... | 2015 | 26352201 |
| in vivo evolution of bacterial resistance in two cases of enterobacter aerogenes infections during treatment with imipenem. | infections caused by multidrug resistant (mdr) bacteria are a major concern worldwide. changes in membrane permeability, including decreased influx and/or increased efflux of antibiotics, are known as key contributors of bacterial mdr. therefore, it is of critical importance to understand molecular mechanisms that link membrane permeability to mdr in order to design new antimicrobial strategies. in this work, we describe genotype-phenotype correlations in enterobacter aerogenes, a clinically pro ... | 2015 | 26398358 |
| mutational and computational evidence that a nickel-transfer tunnel in ured is used for activation of klebsiella aerogenes urease. | nickel-containing urease from klebsiella aerogenes requires four accessory proteins for proper active site metalation. the metallochaperone uree delivers nickel to ureg, a gtpase that forms a ured/uref/ureg complex, which binds to urease apoprotein via ured. prior in silico analysis of the homologous, structurally characterized ureh/uref/ureg complex from helicobacter pylori identified a water tunnel originating at a likely nickel-binding motif in ureg, passing through uref, and exiting ureh, su ... | 2015 | 26401965 |
| composition, in vitro cytotoxic, and antimicrobial activities of the flower essential oil of diospyros discolor from taiwan. | this study investigated the chemical composition, in vitro cytotoxic, and antimicrobial activities of the essential oil isolated from the flower of diospyros discolor from taiwan. the essential oil was isolated using hydrodistillation in a clevenger-type apparatus, and characterized by gc-fid and gc-ms. twenty-eight compounds were identified, representing 100% of the oil. the main components identified were (2z,6e)-farnesol (35.0%), α-cadinol (10.9%), (e)-nerolidol (6.9%), α-humulene (6.0%), τ-c ... | 2015 | 26411038 |
| astaxanthin from psychrotrophic sphingomonas faeni exhibits antagonism against food-spoilage bacteria at low temperatures. | food production and processing industry holds a perpetual relationship with microorganisms and their by-products. in the present study, we aimed to identify beneficial cold-adapted bacteria devoid of any food spoilage properties and study their antagonism against common food-borne pathogens at low temperature conditions. ten isolates were obtained on selective isolation at 5 °c, which were spread across genera pseudomonas, sphingomonas, psychrobacter, leuconostoc, rhodococcus, and arthrobacter. ... | 2015 | 26411893 |
| pan-genomic analysis to redefine species and subspecies based on quantum discontinuous variation: the klebsiella paradigm. | various methods are currently used to define species and are based on the phylogenetic marker 16s ribosomal rna gene sequence, dna-dna hybridization and dna gc content. however, these are restricted genetic tools and showed significant limitations. | 2015 | 26420254 |
| purification and antibiofilm activity of ahl-lactonase from endophytic enterobacter aerogenes vt66. | the opportunistic pathogen pseudomonas aeruginosa uses biofilm lifestyle to resist antibiotic treatment. in our study, endophytic bacterium enterobacter aerogenes vt66 quenched the n-acyl homoserine lactone (ahl) molecules produced by p. aeruginosa pao1. the quorum quenching activity was attributed to the presence of ahl-lactonase. the ahl-lactonase was purified using column chromatography and purified ahl-lactonase was applied for the control of biofilm formation in p. aeruginosa pao1. the resu ... | 2015 | 26432367 |
| activity of temocillin, mecillinam, ceftazidime, and ceftazidime/avibactam against carbapenem-non-susceptible enterobacteriaceae without carbapenemase production. | treatment options for multidrug-resistant gram-negative infections are scarce and therefore alternatives with a narrow spectrum or new agents are sought. antimicrobial susceptibility to temocillin, mecillinam, ceftazidime, and ceftazidime/avibactam was determined using etest and disk diffusion according to european committee on antimicrobial susceptibility testing (eucast) methodology. a total of 77 carbapenem-nonsusceptible enterobacteriaceae were studied, including klebsiella pneumoniae (26%), ... | 2015 | 26433746 |
| epidemiology of carbapenem-resistant enterobacteriaceae in 7 us communities, 2012-2013. | carbapenem-resistant enterobacteriaceae (cre) are increasingly reported worldwide as a cause of infections with high-mortality rates. assessment of the us epidemiology of cre is needed to inform national prevention efforts. | 2015 | 26436831 |
| catabolism of amino acids and related compounds. | this review considers the pathways for the degradation of amino acids and a few related compounds (agmatine, putrescine, ornithine, and aminobutyrate), along with their functions and regulation. nitrogen limitation and an acidic environment are two physiological cues that regulate expression of several amino acid catabolic genes. the review considers escherichia coli, salmonella enterica serovar typhimurium, and klebsiella species. the latter is included because the pathways in klebsiella specie ... | 2005 | 26443507 |