Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| overexpression of skp2 in carcinoma of the cervix does not correlate inversely with p27 expression. | s-phase kinase associated protein 2 (skp2) is a member of the f-box family of substrate recognition subunits of scf-ubiquitin ligase complexes and controls progression from g(1)-s-phase by targeting cell cycle regulators such as p21 and p27. its locus is at 5p13, a region of frequent amplification in several cancers including carcinoma of the cervix (cacx). overexpression of skp2 has been observed in many cancers of an advanced stage. we examine the expression of skp2 in 42 invasive cacx and its ... | 2003 | 12704665 |
| stability of archived liquid-based cervical cytologic specimens. | exfoliated cervical cell specimens collected in preservcyt, a methanol-based medium used in thinprep liquid-based cytology, have been archived in epidemiologic studies. however, long-term dna stability and cytologic stability of these biospecimens have not been evaluated. | 2003 | 12704688 |
| determination of features indicating progression in atypical squamous cells with undetermined significance: human papillomavirus typing and dna ploidy analysis from liquid-based cytologic samples. | the bethesda system of cervical cytologic findings introduced the term ascus (atypical squamous cells of undetermined significance) to cover the broad zone separating normal cytomorphology from definitive squamous intraepithelial lesions (sils). the management of patients with ascus is particularly problematic as approximately 10% of ascus patients develop sil and 1 per 1000 develop cervical carcinoma. | 2003 | 12704691 |
| dual role of tumor suppressor p53 in regulation of dna replication and oncogene e6-promoter activity of epidermodysplasia verruciformis-associated human papillomavirus type 8. | human papillomavirus 8 (hpv8) is a representative of epidermodysplasia verruciformis (ev)-associated viruses. transient assays in the human skin keratinocyte cell line rts3b have shown that its replication depends in trans on expression of the viral proteins e1 and e2, similarly to other hpvs. using deletion mutants and cloned subfragments of the noncoding region (ncr) of hpv8 we identified a 65-bp sequence in the 3' part of the ncr to be necessary and sufficient to support replication in cis. t ... | 2003 | 12706078 |
| mutant p53 can delay growth arrest and loss of cdk2 activity in senescing human fibroblasts without reducing p21(waf1) expression. | functional wild-type p53 is required for human diploid fibroblasts (hdf) to enter an irreversible growth arrest known as replicative senescence. experimentally, abrogation of p53 function by expression of human papillomavirus type 16 e6 or disruption of a key downstream effector p21 by homologous recombination both extended hdf life span. however, although sufficient to extend life span, p21 down-regulation is not necessary, because expression of a dominant-negative mutant p53 (143(ala)) extends ... | 2003 | 12706118 |
| human papillomavirus in virgins and behaviour at risk. | human papillomavirus (hpv) infection is one of the most common sexual transmitted diseases (stds). we compared two groups of virgins with genital hpv lesions to evaluate the behaviour at risk in the transmission of hpv infection. partners were also examined. hpv lesions were detected in 88 virgins, who have never had sexual intercourse. this can be due to vertical transmission, fomities and skin-to-skin contact. many other hypothesis can be proposed to explain hpv genital infection, however, fur ... | 2003 | 12706855 |
| [risk factors for cervico-uterine cancer associated to hpv: p53 codon 72 polymorphism in women attending hospital care]. | in codon 72 of the p53 antioncogene there are two alleles, arginine and proline; the arg/arg genotype has recently been identified as a risk factor for developing of cervicouterine cancer (cuca) associated to human papillomavirus (hvp) infection. the aim of this work was to determine in a sample of women the frequency of proline-arginine alleles and genotypes of p53 codon 72. the study was conducted in a sample of inpatient women at the hospital. p53 codon 72 alleles were determined in genomic a ... | 2003 | 12708345 |
| hpv oligonucleotide microarray-based detection of hpv genotypes in cervical neoplastic lesions. | in this study we examined the use of a new-human papillomavirus (hpv) detection method, the hpv oligonucleotide microarray system (biomedlab co., korea), which we compared with the well-established hpv dna detection system (hybrid capture ii; hc-ii, digene co.). this new method prompted us to develop a new hpv genotyping technique, using the oligonucleotide microarray, to detect the generic and type-specific sequence of hpv types. in particular, we undertook the evaluation of the clinical effica ... | 2003 | 12713982 |
| induction of tumor-specific cytotoxicity in tumor infiltrating lymphocytes by hpv16 and hpv18 e7-pulsed autologous dendritic cells in patients with cancer of the uterine cervix. | to evaluate the potential of autologous dendritic cells (dc) pulsed with hpv16 and hpv18 e7 oncoprotein in restoring tumor-specific cytotoxicity in populations of tumor infiltrating lymphocytes (til) for adoptive immunotherapy of cervical cancer patients. | 2003 | 12713991 |
| prevalence of and risk factors for viral infections among human immunodeficiency virus (hiv)-infected and high-risk hiv-uninfected women. | viruses that can persist in the host are of special concern in immunocompromised populations. among 871 human immunodeficiency virus (hiv)-infected and 439 high-risk hiv-uninfected women, seroprevalences of cytomegalovirus, hepatitis b virus, hepatitis c virus, and herpes simplex virus types 1 and 2 and prevalence of human papillomavirus dna in cervicovaginal lavage fluids were all >50% and were 2-30 times higher than prevalences in the general population. prevalences were highest among hiv-infe ... | 2003 | 12717619 |
| the prevalence of human papillomavirus in cervical cancer in iran. | background: the human papiloma virus (hpv), which is sexually transmitted, and most commonly causes genital warts, has been linked to cervical intraepithelial neoplasia and invasive carcinoma. of ninety plus types of hpv, hpv-16 is the most prevalent in cervical cancer, followed by hpv-18, and hpv-33. as hpv's implication has not been assessed in the middle east the main focus of this retrospective study was to determine the prevalence of hpv -16,18, and 33 in cases of cervical cancer from iran. ... | 2002 | 12718611 |
| [frequency of hpv infection of the uterine cervix among perimenopausal women in wielkopolska region]. | human papillomavirus (hpv) is one of the main causes of cervical neoplasia. there is now consistent evidence that human papillomavirus (hpv) has a causal role in the etiology of cervical cancer and that sexual habits and reproductive/hormonal factors are associated with the risk of invasive cervical cancer. in our investigation we estimated the frequency of hpv infection in uterine cervix in women gathered in perimenopausal and postmenopausal groups. | 2002 | 12722378 |
| [definitive role of polar residue clusters in b-dna major groove recognition by protein factors]. | the 3d structural data for a number of protein-dna complexes were used to analyze the regions of specific contact with the major groove of b-dna double helix. the set included seven nonhomologous complexes featuring 12 dna-binding domains of transcription factors and regulatory factors. the protein domains differed in structure, contained different motifs in the binding region, and broadly varied in chain length, from 30 to 200 residues. protein-dna interaction was assessed as hydrogen bonding b ... | 2003 | 12723474 |
| infection with the oncogenic human papillomavirus type 59 alters protein components of the cornified cell envelope. | infection of the genital tract with human papillomaviruses (hpvs) leads to proliferative and dysplastic epithelial lesions. the mechanisms used by the virus to escape the infected keratinocyte are not well understood. infection of keratinocytes with hpv does not cause lysis, the mechanism used by many viruses to release newly formed virions. for hpv 11, a type associated with a low risk of neoplastic disease, the cornified cell envelope (cce) of infected keratinocytes is thin and fragile, and tr ... | 2003 | 12726726 |
| anal dysplasia in men who have sex with men. | risk factors for anal cancer include anal intercourse and infection with multiple strains of human papillomavirus, the causative agent of anal precancerous dysplasia. several recent studies have shown that hiv-seropositive gay men are at greater risk for anal dysplastic lesions than seronegative gay men. moreover, the risk for detection and progression of dysplastic lesions grows as the cd4+ cell count declines. a surgeon with a practice that includes gay men referred for anorectal disease prese ... | 1999 | 12728907 |
| inhibition of human papillomavirus dna replication by small molecule antagonists of the e1-e2 protein interaction. | human papillomavirus (hpv) dna replication is initiated by recruitment of the e1 helicase by the e2 protein to the viral origin. screening of our corporate compound collection with an assay measuring the cooperative binding of e1 and e2 to the origin identified a class of small molecule inhibitors of the protein interaction between e1 and e2. isothermal titration calorimetry and changes in protein fluorescence showed that the inhibitors bind to the transactivation domain of e2, the region that i ... | 2003 | 12730224 |
| meta-analysis of social inequality and the risk of cervical cancer. | previous studies of the relationship between socio-economic status and cervical cancer have been mainly based on record linkage of routine data, such as cancer registry incidence rates and regional measures of social class based on census data. these routine data are liable to substantial misclassification with respect to socio-economic status. previous reports are also primarily from developed countries, whereas the major burden of cervical cancer is in developing countries. we have therefore p ... | 2003 | 12740919 |
| establishment and characterization of rat progenitor hair cell lines. | cochlear progenitor hair cell lines are useful for studies of cellular specification, gene expression features, and signal transduction involved in the development of hair cells. to obtain embryonic and postnatal cochlear progenitor hair cell lines, we immortalized primary cultures of sensorineural epithelial cells from otocysts on embryonic day 12 (e12) and explants of the organ of corti tissues on postnatal day 5 (p5). primary cultures and explants were then transduced by the e6/e7 genes of hu ... | 2003 | 12742237 |
| activation of adenovirus early promoters and lytic phase in differentiated strata of organotypic cultures of human keratinocytes. | human oncolytic adenoviruses have been used in clinical trials targeting cancers of epithelial origin. to gain a better understanding of the infectious cycle of adenovirus in normal human squamous tissues, we examined the viral infection process in organotypic cultures of primary human keratinocytes. we show that for the infection to occur, wounding of the epithelium is required. in addition, infection appears to initiate at the basal or parabasal cells that express the high-affinity coxsackievi ... | 2003 | 12743310 |
| classification of human papillomavirus. | 2003 | 12748326 | |
| routine genotyping of human papillomavirus samples in denmark. | in order to examine a sensitive unbiased consensus pcr with routine sequencing for hpv typing, we analysed danish male and female patients suspected of having an hpv infection. we used the well-characterised nested pcr setting with my09/my11 and gp5+/gp6+ primers, followed by routine cycle sequencing. of 1283 clinical samples from female patients based on suspected hpv infection, we found 379 (29%) negatives and 894 (70%) positives. samples containing >5000 hpv copies/ml were genotyped by sequen ... | 2003 | 12752219 |
| multiple human papillomavirus dna identified in verruciform xanthoma by nested polymerase chain reaction with degenerate consensus primers. | 2003 | 12753178 | |
| human papillomaviruses type 16+ and 18+ cervical carcinoma cells are sensitive to staurosporine-mediated apoptosis. | we have recently shown that staurosporine (st) can trigger apoptosis of caski and hela cervical tumor cells from g2/m checkpoint, though the mechanism remains elusive. in this study, we reported that st induced the inhibition of e6 and e7 viral oncogene and mdm2 expression, while it led to increased levels of p53, which was transiently located to mitochondria. additionally, the proteins of the p53-regulated genes, p21(waf1) and bax, were increased with a similar time, while bcl-2 and bcl-x(l) ex ... | 2003 | 12753950 |
| [genotyping of human papillomavirus genotyes in cervical scrapes by line probe assay]. | to pursue the further development of a practical hpv genotyping system applicable for mass screening. | 1999 | 12759942 |
| [study on the association between squamous cell carcinoma of larynx and hpv subgene by pcr]. | to measure the human papilloma virus(hpv) hpv-e1 in squamous cell carcinoma of larynx. | 1999 | 12764861 |
| brca1 associates with human papillomavirus type 18 e2 and stimulates e2-dependent transcription. | brca1 is a breast and ovarian cancer-related tumor suppressor and has a role in transcriptional activation. we show here that brca1 enhances human papillomavirus type 18 (hpv-18) e2-dependent transcription in vivo. using biochemical approaches, we discovered that brca1 interacts with the carboxyl-terminus of hpv-18 e2 protein in vivo and in vitro. point mutations in the c-terminus make brca1 defective in transcriptional activation in e2-dependent transcription. this finding suggests that the c-t ... | 2003 | 12767931 |
| [the study of human papilloma viruses infection in juvenile pharynlaryngeal papillomatosis by pcr and dot blot hybridization]. | to study the relationship between infection of human papillomavirus and juvenile pharynlaryngeal papilloma. | 2000 | 12768707 |
| inclusion of hpv testing in routine cervical cancer screening for women above 29 years in germany: results for 8466 patients. | in a prospective cohort study 8466 women attending routine cervical cancer screening were recruited. colposcopy was performed on women with any degree of atypia on cytology and/or a positive high-risk human papillomavirus (hpv)-dna test (hc2; hybrid capture 2((c))), and for a randomly selected sample of 3.4% women with negative findings on both. quality control included reviews of cytology, histology, colposcopy images and retesting of samples with polymerase chain reaction. test diagnostic perf ... | 2003 | 12771924 |
| cervical dysplasia: early intervention. | cervical cancer is the second-most common cancer in young women and is one of the most common causes of cancer deaths among women, particularly in minorities and in impoverished countries. cervical dysplasia, a premalignant lesion that can progress to cervical cancer, is caused primarily by a sexually transmitted infection with an oncogenic strain of the human papillomavirus (hpv). not all women with the virus develop cervical dysplasia or cervical cancer. it has been postulated there are multip ... | 2003 | 12777161 |
| folate, vitamin b12, and homocysteine status. findings of no relation between human papillomavirus persistence and cervical dysplasia. | human papillomavirus (hpv) infections are the cause of most, if not all, cervical cancers. women consistently positive for oncogenic type hpv infections have a greater risk of developing cervical dysplasia compared with women transiently infected. hpv infection alone appears to be insufficient to produce disease, suggesting that other cofactors may be needed. folate, vitamin b12, and homocysteine, through their role in dna methylation, may be involved in cervical neoplasia. | 2003 | 12781848 |
| rna interference for treating cancers caused by viral infection. | the newly discovered phenomenon of rna interference (rnai) offers the dual facility of selective viral gene silencing coupled with ease of tailoring to meet genetic variation within the viral genome. such promise identifies rnai as an exciting new approach to treat viral-induced diseases, including viral-induced cancers (e.g. cervical carcinoma, hepatocellular carcinomas and haematopoietic and lymphoid malignancies). cervical cancer is the second most common cancer in women worldwide and is caus ... | 2003 | 12783614 |
| lichen sclerosus is frequently present in penile squamous cell carcinomas but is not always associated with oncogenic human papillomavirus. | penile squamous cell carcinoma (scc) may occur on pre-existing lesions of lichen sclerosus (ls). however, the prevalence of histological changes of ls in penile scc is not well established. moreover, mucosal oncogenic human papillomaviruses (hpvs) are sometimes detected in penile scc, but have not been systematically sought in ls-associated penile scc. | 2003 | 12786823 |
| genital carriage of human papilloma virus (hpv) dna in prepubertal girls with and without vulval disease. | human papilloma virus (hpv) can reach a child's anogenital area by vertical transmission or by close contact, which can be either sexual or nonsexual. our objective was to compare hpv in prepubertal girls with and without lichen sclerosus (ls). we compared the frequencies and types of hpv in girls with ls with those in children with non-ls vulval disease (vulval swab and urine) and in children with no known vulval disease (urine only). hpv dna was detected using a nested polymerase chain reactio ... | 2003 | 12787264 |
| cervical human papillomavirus infection in tunisian women. | it is well established that certain types of human papillomavirus (hpv) are the sexually transmitted agents etiologically linked to cervical cancer. sexual habits have been shown to be a major determining factor for hpv infection. a large study was carried out to investigate the prevalence and risk factors associated with cervical infection with hpv in tunisian women. | 2003 | 12789471 |
| oncoprotein expression of e6 and e7 does not prevent 5-fluorouracil (5fu) mediated g1/s arrest and apoptosis in 5fu resistant carcinoma cell lines. | 5-fluorouracil (5fu) exposure can lead to both g1/s arrest and apoptosis induction which are dependent of p53 induction. the human papilloma virus oncoproteins (hpv), e6 and e7, inactivate respectively p53 and rb. p53 degradation by e6 protein, leads to lack of g1/s arrest after genotoxic stress. overexpression of e7 protein prevents p53-induced g1/s arrest following dna damage. however, few studies have described 5fu effect and efficacy on cancer cell lines presenting hpv 18 positive status. kb ... | 2003 | 12792779 |
| human papillomavirus and cervical cancer: not just a sexually transmitted disease. | the human papillomavirus (hpv) is the cause of virtually all cancers of the cervix, the fourth most common cancer in women in the united states. hpv is sexually transmitted, and the lifetime risk of contracting the virus is estimated to be 75%-90%. new methods of detecting hpv infection and cellular changes (dysplasia) caused by hpv can greatly reduce the mortality associated with this virus. more than 100 types of hpv exist and may be classified as low-, intermediate-, or high-risk in terms of ... | 2003 | 12793333 |
| enhanced mucosal and systemic immune responses following intravaginal immunization with human papillomavirus 16 l1 virus-like particle vaccine in thermosensitive mucoadhesive delivery systems. | to develop more potent and convenient mucosal human papillomavirus (hpv) vaccines, we tested the effect of thermosensitive mucoadhesive vaginal vaccine delivery systems on the local and systemic antibody responses to hpv 16 l1 virus-like particles (vlp). hpv 16 l1 vlp expressed from recombinant baculovirus-infected sf21 insect cells were delivered in phosphate-buffered saline (pbs) or thermosensitive mucoadhesive delivery systems, composed of poloxamers (pol) and varying amounts of polyethylene ... | 2003 | 12794729 |
| interplay between human papillomaviruses and dendritic cells. | the design of the human papillomavirus (hpv) infection cycle is tightly fitted to the differentiation program of its natural host, the keratinocyte. this has important consequences for the role of antigen-presenting cells in the priming of antiviral immunity. the confinement of hpv infection to epithelia puts the epithelial dendritic cell, the langerhans cell (lc), in charge of the induction of t cell-dependent immunity. because hpv-infected keratinocytes cannot reach the regional lymphoid organ ... | 2003 | 12797450 |
| correlates of il-10 and il-12 concentrations in cervical secretions. | interindividual variations in host immune responses to hpv infection are thought to be important determinants of viral persistence and progression to cervical intraepithelial neoplasia and cancer. however, few studies have measured local immune markers at the site of infection (e.g., the cervical mucosa). we sought to determine biologic correlates of il-10 and il-12 concentrations in cervical secretions. cervical secretions were passively collected using a weckcel sponge from 247 women participa ... | 2003 | 12797539 |
| morphologic and biologic studies on ten cases of verrucous carcinoma of the vulva supporting the theory of a discrete clinico-pathologic entity. | ten cases of verrucous carcinoma (vc) of the vulva diagnosed from january 1989 to december 1996 were studied. patient age ranged from 50 to 83 years. the following examinations were performed on buffered formalin-fixed material: 1). in situ dna hybridization, probes hpv 6/11, 16/18, 31/35/51; and 2). a series of immunohistochemical stainings to demonstrate wild and mutant types of the p53 protein, cytokeratin expression and pattern distribution (ae1 and ae3), and proliferating pattern (mib 1). i ... | 2003 | 12801263 |
| p53 loss of function enhances genomic instability and accelerates clonal evolution of murine myeloid progenitors expressing the p(210)bcr-abl tyrosine kinase. | the p210 bcr-abl fusion protein has a key role in the pathogenesis of chronic myeloid leukemia (cml). however, its influence on disease progression to blast crisis is marginal and mostly due to its effect of impairing the genomic stability of clonal myeloid progenitors through pathways still largely unknown. | 2003 | 12801837 |
| non-hiv antivirals - a review of the recent patent literature. | this review covers the non-hiv antiviral patent literature from december 2001 to april 2002. most of the patent applications describe new compounds for the treatment of hepatitis c virus (hcv) by inhibition of the ns3 serine protease. several examples of both nucleoside and non-nucleoside inhibitors of the hcv polymerase ns5b have been reported. hepatitis b virus (hbv) therapy continues to be dominated by nucleoside analogs, but several non-nucleoside hbv polymerase inhibitors have also been rep ... | 2002 | 12802698 |
| attitudes about human papillomavirus vaccine in young women. | human papillomavirus (hpv) vaccines are under investigation, but little is known about attitudes regarding vaccination. the aims of this study were to identify attitudes about and intention to receive an hpv vaccine in young women using a theory-based model. young women (n=52, mean age 25 years, range 18-30 years, 35% black/non-hispanic) completed a survey assessing knowledge, attitudes about hpv vaccination, and risk behaviours. associations between attitudes and intention to receive the vaccin ... | 2003 | 12803935 |
| heterologous boosting increases immunogenicity of chimeric papillomavirus virus-like particle vaccines. | chimeric human papillomavirus virus-like particles (hpv cvlps), containing the hpv16 non-structural protein e7, are potent vaccines for inducing antigen-specific protective immunity against hpv-transformed tumors in animal models. previous data demonstrated that the effectiveness of cytotoxic t lymphocyte (ctl) induction after repetitive vaccination with the same cvlp, and thus vaccine efficacy, is limited by the presence of neutralizing antibodies induced after the first application. here, we d ... | 2003 | 12804851 |
| transcriptional regulation of the telomerase htert gene as a target for cellular and viral oncogenic mechanisms. | malignant transformation from mortal, normal cells to immortal, cancer cells is generally associated with activation of telomerase and subsequent telomere maintenance. a major mechanism to regulate telomerase activity in human cells is transcriptional control of the telomerase catalytic subunit gene, human telomerase reverse transcriptase (htert). several transcription factors, including oncogene products (e.g. c-myc) and tumor suppressor gene products (e.g. wt1 and p53), are able to control hte ... | 2003 | 12807729 |
| chapter 4: genital tract infections, cervical inflammation, and antioxidant nutrients--assessing their roles as human papillomavirus cofactors. | cervical infections by approximately 15 human papillomavirus (hpv) types are the necessary cause of cervical cancer and its immediate precursor lesions. however, oncogenic hpv infections are usually benign and usually resolve within 1-2 years. a few of these infections persist and progress to cervical precancer and cancer. a number of cervical factors, such as infection by sexually transmitted pathogens other than hpv, cervical inflammation, and antioxidant nutrients, may influence the natural h ... | 2003 | 12807942 |
| chapter 7: human papillomavirus and cancer of the upper aerodigestive tract. | we discuss current evidence of the role of human papillomavirus (hpv) in some cancers of the upper aerodigestive tract, including the oral cavity, the pharynx, and the larynx. cancers of the oral cavity and the pharynx are associated mainly with tobacco and alcohol exposure, but there is evidence from case series, from case-control studies, and from cohort studies that hpv plays a role in a fraction of these cancers, particularly cancer in the oropharynx and tonsil. the hpv type most commonly as ... | 2003 | 12807945 |
| chapter 10: cervical cancer screening using visualization techniques. | there is a resurgence of interest in the use of visual techniques to identify cervical intraepithelial neoplasia (cin). these visual techniques can be divided into two general categories. one is the simple visual screening method, such as direct visual inspection (dvi), during which the cervix is visualized with either the naked eye or a low-power magnification device after the application of a solution of 3% to 5% acetic acid that is used as a chemical contrast agent to highlight regions of cin ... | 2003 | 12807948 |
| chapter 13: primary screening of cervical cancer with human papillomavirus tests. | despite its history of success in cancer screening, pap cytology has important limitations, particularly its high false-negative rate, which carries important public health implications. since the mid-1990s, there has been substantial interest in the use of human papillomavirus (hpv) dna testing in cervical cancer screening under the premise that the testing of cervical cells for the causative agent of cervical cancer could have acceptable screening performance, while being more reproducible in ... | 2003 | 12807951 |
| analysis of pregnancy and other factors on detection of human papilloma virus (hpv) infection using weighted estimating equations for follow-up data. | generalized estimating equations have been well established to draw inference for the marginal mean from follow-up data. many studies suffer from missing data that may result in biased parameter estimates if the data are not missing completely at random. robins and co-workers proposed using weighted estimating equations (wee) in estimating the mean structure if drop-out occurs missing at random. we illustrate the differences between the wee and the commonly applied available case analysis in a s ... | 2003 | 12820285 |
| detection and typing of hpv genotypes in various cervical lesions by hpv oligonucleotide microarray. | this study was conducted to evaluate a clinical efficacy of human papillomavirus (hpv) oligonucleotide microarray (biomedlab co., seoul, south korea) for the detection of hpvs in various cervical lesions. | 2003 | 12821341 |
| iron withdrawal strategies fail to prevent the growth of siha-induced tumors in mice. | cervical carcinoma is a human papillomavirus (hpv)-associated cancer for which treatment options still mainly rely on surgical procedures, with or without adjuvant radiotherapy and chemotherapy. we have previously shown that the chemically unrelated iron chelators desferrioxamine and deferiprone inhibit the growth and induce the apoptosis of hpv-positive cervical carcinoma cell lines, suggesting that iron chelators may represent a potential therapeutic approach for the treatment of cervical carc ... | 2003 | 12821347 |
| second primary cancers after cervical intraepithelial neoplasia iii and invasive cervical cancer in southeast england. | multiple primary cancers may arise in an individual because they share a common environmental risk factor (such as smoking); genetic predisposition or immunodeficiency may predispose to both cancers, or treatment for one cancer may cause a second cancer. the objective of this analysis was to identify which, if any, cancers occur more often than would be expected in a cohort of women diagnosed with cervical intraepithelial neoplasia iii (cin iii) and in women with invasive cervical cancer. | 2003 | 12821353 |
| hpv e7 expression in skeletal muscle cells distinguishes initiation of the postmitotic state from its maintenance. | the e7 oncogene is an essential tool used by papillomaviruses to interfere with the cell cycle and cellular differentiation. we investigated the effects of e7 expression on both cellular functions in skeletal muscle cells, a terminally differentiating system. when expressed in myoblasts, e7 impaired differentiation only partially, but allowed continuation of dna synthesis during and after differentiation. surprisingly, e7 expression in terminally differentiated myotubes could not reactivate dna ... | 2003 | 12821937 |
| [the role of papilloma virus in development of cervix precancer and cancer (a literature review)]. | there are now enough data confirming the causal relationship between the papilloma-virus infection and the lesion of the neck of uterus. virtually all dysplasias contain the human papilloma virus either as an infection virion or as episome-type or integrated dna. the chronic papilloma-virus infection of high risk is an indispensable precondition for creating a precancerous and malignant phenotypes. however, considering the human papilloma-virus infection to be the key risk factor of precancerosi ... | 2003 | 12822310 |
| skin and subcutaneous infections. | cutaneous infections most commonly are caused by staphylococcus aureus and gabhs. life-threatening complications can occur and are preventable with early diagnosis and specific therapy. dermatophytoses frequently are seen in children and immune-compromised adults with poor hygiene. sporotrichosis and nontuberculous mycobacteria are ubiquitous and can cause indolent granulomatous lesions. vesicular and fleshy lesions are manifestations of cutaneous viral infections due to hsv, varicella-zoster vi ... | 2003 | 12825248 |
| environmental exposure and lung cancer among nonsmokers: an example of taiwanese female lung cancer. | lung cancer is the leading cause of cancer death worldwide and in taiwan. cigarette smoking is considered to be the most important risk factor, since about 90% of lung cancer can be related to cigarette smoking. despite the recent decrease of cigarette smoking, lung cancer is still the leading cause of cancer death in the united states. in taiwan, only around 50% of lung cancer incidence could be associated with cigarette smoking, particularly less than 10% of taiwanese women are smokers. thus, ... | 2003 | 12826030 |
| vl position 34 is a key determinant for the engineering of stable antibodies with fast dissociation rates. | predictive engineering of antibodies exhibiting fast kinetic properties could provide reagents for biotechnological applications such as continuous monitoring of compounds or affinity chromatography. based on covariance analysis of murine germline antibody variable domains, we selected position l34 (kabat numbering) for mutational studies. this position is located at the vl/vh interface, at the base of the paratope but with limited antigen contacts, thus making it an attractive position for mild ... | 2003 | 12826730 |
| human papillomavirus dna in oral squamous cell carcinomas and normal oral mucosa. | to elucidate the putative etiologic role of human papillomaviruses (hpv) in oral carcinogenesis, a comparative study was carried out on 62 tissue specimens of oral squamous cell carcinoma (oscc) and on 62 specimens of histologically normal oral mucosa obtained from the individuals who matched the subjects with oscc in age, gender, localization of obtained tissue specimens, drinking and smoking habits. internal control amplification showed that amplifiable dna was recovered from 59/62 and 61/62 t ... | 2003 | 12828338 |
| mucosal igg and iga responses to human papillomavirus type 16 capsid proteins in hpv16-infected women without visible pathology. | human papillomavirus type 16 (hpv16) may infect the cervical epithelium without producing pathological changes for a long time. to investigate if mucosal antibodies are induced in hpv16-infected women without visible pathology, cervical mucus from hpv16-infected patients with and without evident pathology, along with mucus from uninfected women were analyzed for the presence of mucosal igg and secretory iga (siga) antibodies to hpv16 capsid proteins by elisa. siga and igg antibodies were found i ... | 2003 | 12828867 |
| successful treatment of bowenoid papulosis in a 9-year-old girl with vertically acquired human immunodeficiency virus. | a 9-year-old black girl with vertically acquired human immunodeficiency virus (hiv) and no history of condyloma acuminata presented with a 4-year history of enlarging and spreading dark brown flat papules in the perineum. some of the lesions were confluent and extended from the clitoris to the labia majora and posteriorly to the buttocks and perianal region. a biopsy of one of the lesions showed bowenoid features. our patient had a normal pap smear, but vaginal and cervical biopsy specimens reve ... | 2003 | 12837910 |
| chlamydia trachomatis, herpes simplex virus 2, and human t-cell lymphotrophic virus type 1 are not associated with grade of cervical neoplasia in jamaican colposcopy patients. | a few recent studies have suggested that other sexually transmitted infections may increase the likelihood of a human papillomavirus (hpv) infection progressing to high-grade cervical neoplasia and cancer. | 2003 | 12838087 |
| risk of cancer in persons with aids in italy, 1985-1998. | a record linkage was carried out between the italian registry of aids and 19 cancer registries (crs), which covered 23% of the italian population, to estimate the overall cancer burden among persons with hiv or aids (pwha) in italy, according to various characteristics. observed and expected numbers of cancer and standardised incidence ratios (sirs) were assessed until 1998 in 12 104 pwha aged 15-69 years, for a total of 60 421 person-years. significantly increased sirs were observed for kaposi' ... | 2003 | 12838307 |
| centrosomes, genomic instability, and cervical carcinogenesis. | high-risk human papillomavirus (hpv)-associated carcinogenesis of the uterine cervix is a particularly useful model to study basic mechanisms of genomic instability in cancer. cervical carcinogenesis is associated with the expression of two high-risk hpv-encoded oncoproteins, e6 and e7. aneuploidy, the most frequent form of genomic instability in human carcinomas, develops as early as in nonmalignant cervical precursor lesions. in addition, cervical neoplasia is frequently associated with abnorm ... | 2003 | 12839094 |
| immortalization of normal human embryonic fibroblasts by introduction of either the human papillomavirus type 16 e6 or e7 gene alone. | the ability of the human papillomavirus type 16 (hpv-16) e6 or e7 gene to induce immortalization of normal human embryonic fibroblast whe-7 cells was examined. whe-7 cells at 9 population doublings (pd) were infected with retrovirus vectors encoding either hpv-16 e6 or e7 alone or both e6 and e7 (e6/e7). one of 4 isolated clones carrying e6 alone became immortal and is currently at >445 pd. four of 4 isolated clones carrying e7 alone escaped from crisis and are currently at >330 pd. three of 5 i ... | 2003 | 12845665 |
| effect of plasma micronutrients on clearance of oncogenic human papillomavirus (hpv) infection (united states). | data have suggested that higher levels of nutrients are protective against hpv persistence and cervical neoplasia. this study assessed the role of circulating nutrients on clearance of oncogenic hpv infections. | 2003 | 12846362 |
| cytogenetic and molecular genetic changes in malignant transformation of immortalized esophageal epithelial cells. | the purpose of this study was to evaluate the extent to which the expression of p53, c-myc, bcl-2, ras genes and chromosomes, along with activity of htert, impacts on the malignant transformation of immortalized esophageal epithelial cells. the shee cell line was established from an embryonic esophageal epithelial cell induced by transduction of e6e7 genes of human papillomavirus type 18 (hpv18e6e7). in cells of the 85th passage (shee85), the malignant transformation of shee was confirmed by mor ... | 2003 | 12851721 |
| plantar wart treatment with combination imiquimod and salicylic acid pads. | treatment of plantar warts is often difficult and may be painful, often employing destructive treatment modalities. we report the successful treatment of a patient with a large plantar wart using imiquimod 5% cream under occlusion with a 40% salicylic acid pad. this combination treatment modality likely allows successful delivery of imiquimod through the thick skin on the plantar surface. once penetrated, an anti-viral state is created by upregulating specific cytokines to eradicate the human pa ... | 2003 | 12852385 |
| use of sam2 biotin capture membrane in microarrayed compound screening (muarcs) format for nucleic acid polymerization assays. | microarrayed compound screening format (muarcs) is a novel high-throughput screening technology that uses agarose matrices to integrate various biochemical or biological reagents in the assay. to evaluate the feasibility of using the muarcs technology for nucleic acid polymerization assays, the authors developed hiv reverse transcription (rt) and e1-dependent human papillomavirus (hpv) replication assays in this format. hiv rt is an rna-dependent dna polymerase, whereas hpv e1 is a dna helicase. ... | 2003 | 12857381 |
| detection of human papillomavirus in urine and cervical swabs from patients with invasive cervical cancer. | despite the high prevalence of both human papillomavirus (hpv) infections and cervical cancer among zimbabwean women, the ability to test for hpv infection of the uterine cervix is limited by a lack of an easy sample collection method that does not require gynecological examination. the presence of hpvs in urine and cervical swab samples collected from 43 women who presented with invasive cervical cancer was investigated. hpv detection was done by means of degenerate primers in a nested polymera ... | 2003 | 12858416 |
| human papillomavirus type 16 and immune status in human immunodeficiency virus-seropositive women. | human papillomavirus (hpv) type 16 is etiologically associated with approximately half of all cervical cancers. it is important, therefore, to determine the characteristics that distinguish hpv16 from other hpv types. a preliminary result based on cross-sectional baseline data in the women's interagency human immunodeficiency virus (hiv) study (wihs) suggested that the prevalence of hpv16 might have a weaker association with immune status in hiv-seropositive women than that of other hpv types. t ... | 2003 | 12865452 |
| preferential nuclear localization of the human papillomavirus type 16 e6 oncoprotein in cervical carcinoma cells. | the e6 protein of the high-risk human papillomavirus type 16 (hpv-16) is involved in the tumorigenesis of human cervical cells by targeting numerous cellular proteins. we characterized new anti-e6 monoclonal antibodies and used them for precise localization of the e6 oncoprotein within carcinoma cells. overexpressed e6 protein was predominantly detected in the nucleus of transiently transfected hacat cells. while mostly localized at the periphery of condensed chromatin, e6 was also associated wi ... | 2003 | 12867640 |
| prevalence of human papillomavirus infection in rural villages of the bolivian amazon. | cervical cancer constitutes a major health problem in developing countries like bolivia. the roles of certain genotypes of human papillomaviruses (hpvs) in the pathogenesis of cervical cancer is well established. the prevalence of hpv infection among sexually active women varies greatly. information regarding hpv infection in bolivia is very much scarce, specially in regions like the amazonian lowland. we studied 135 healthy women living in four rural localities of the bolivian amazon. presence ... | 2003 | 12870061 |
| findings to date from the ascus-lsil triage study (alts). | controversy exists in the united states regarding the proper evaluation and management of low-grade squamous intraepithelial lesion (lsil) and equivocal (atypical squamous cells of undetermined significance [ascus, now asc-us]) cervical cytologic interpretations. to address this issue, the national cancer institute initiated the ascus-lsil triage study (alts). alts is a multicenter, randomized clinical trial designed to evaluate 3 alternative methods of management, namely, immediate colposcopy, ... | 2003 | 12873166 |
| human papillomavirus reporting: minimizing patient and laboratory risk. | risk management efforts in the cytology laboratory must address the gap between what can be achieved with medical history's most effective cancer screening test, the papanicolaou (pap) test, and even higher entrenched public expectations. data from the atypical squamous cells of undetermined significance (ascus)/low-grade squamous intraepithelial lesion triage study (alts) now provide level i clinical evidence from a large, randomized, controlled, multicenter clinical trial that reflex human pap ... | 2003 | 12873169 |
| successfully integrating human papillomavirus testing into your practice. | integrating human papillomavirus (hpv) testing into the practice of cervical cancer screening is a continuous process that begins and ends with education. an understanding of the basic science and clinical relevance are critical to proper implementation. one must have knowledge of one's own laboratory statistics, clinician awareness, and market forces before the final steps are taken. additional logistical issues are often laboratory specific or state specific, but are addressed here. | 2003 | 12873172 |
| long-term follow-up of patients treated with recombinant human interferon gamma for cervical intraepithelial neoplasia. | the immediate results of interferon gamma (ifn-gamma) treatment in the management of cervical intraepithelial neoplasia (cin) have been described. however, little is known of the long-term results of this conservative treatment and we aimed to assess them. | 2003 | 12873779 |
| quantitative rt-pcr assay for hpv infection in cultured cells. | early events in the life cycle of the human papillomaviruses (hpv) have been difficult to investigate due to both the scarcity of authentic hpv virions and limitations in assays to detect and quantify nonpermissive infections in monolayer cell culture. we have developed a quantitative reverse transcription-pcr (qrt-pcr) assay for the e1( wedge )e4 transcript of hpv-11. this assay is both sensitive, and capable of differentiating between infections caused by a wide range of virus input. the qrt-p ... | 2003 | 12880929 |
| e6/e7 genes of human papilloma virus type 18 induced immortalization of human fetal esophageal epithelium. | to study the role played by human papilloma virus (hpv) in carcinogenesis, immortalized esophageal epithelial cells were induced by e6 and e7 genes of hpv type 18 and the biological behavior was studied. human fetal esophageal epithelial cells were transfected with recombined hpv18e6e7aav and were cultured and passaged in medium m199. in both the 10th passage (shee10) and the 31st passage (shee31), their proliferative rates by flow cytometry and their abilities to grow and form colonies in soft ... | 2003 | 12883719 |
| topical vidarabine or 5-fluorouracil treatment against persistent hpv in genital (pre)cancerous lesions. | in the present study, effectiveness of topical vidarabine or subsequent 5-fluorouracil (5-fu) administration was examined against persistent genital human papillomavirus (hpv) infection after local surgery. thirty patients underwent local eradication treatment of uterine cervical intra-epithelial neoplasia (cin) and stage ia1 uterine cervical cancers. hpv typing was performed by pcr-rflp analysis. hpv infection was detected pre-operatively in 29 of 30 patients. of these, hpv was still present in ... | 2003 | 12883720 |
| oral immunogenicity of human papillomavirus-like particles expressed in potato. | human papillomavirus-like particles (hpv vlps) have shown considerable promise as a parenteral vaccine for the prevention of cervical cancer and its precursor lesions. parenteral vaccines are expensive to produce and deliver, however, and therefore are not optimal for use in resource-poor settings, where most cervical hpv disease occurs. transgenic plants expressing recombinant vaccine immunogens offer an attractive and potentially inexpensive alternative to vaccination by injection. for example ... | 2003 | 12885889 |
| association between high-risk human papillomavirus dna load and precursor lesions of cervical cancer in mexican women. | our objective was to determine the association between viral load of high risk human papilloma virus (hpv) using the hybrid capture ii (hc ii) system and cervical intraepithelial neoplasia (cin) lesion stage. | 2003 | 12893192 |
| human papillomavirus infection in men who have sex with men participating in a dutch gay-cohort study. | to develop strategies for prevention and early treatment of human papillomavirus (hpv) anal and penile cancer, a better understanding of related sexual behavior risk factors is needed. | 2003 | 12897686 |
| [status of screening tests for cervical cancer and its precancerous lesion]. | several screening methods for cervical cancer and its precancerous lesion are reviewed. cervical cancer screening using visual inspection, colposcopy, oncogenic human papillomavirus dna testing, liquid-based monolayers and automated pap smear screening instruments are all potentially valuable when used alone or in combination. newly developed techniques provide an opportunity to extend practical cervical cancer screening to large population in limited resource areas, and help do more cost-effect ... | 2001 | 12901114 |
| expression of midkine in benign, premalignant and malignant vulvar tumors. | to clarify the role of midkine (mk) in vulvar carcinogenesis though examination of its expression in vulvar lesions including vulvar condyloma acuminata (vca), vulvar intraepithelial neoplasia (vin) and vulvar squamous cell carcinomas (vscc), and to analyze the relationship between mk expression and human papilloma virus (hpv) infection. | 2002 | 12901536 |
| evolutionary relationships among seventeen human papillomavirus genotypes. | the dna and the protein sequences of the six major open reading frames (orfs) of 17 human papillomavirus (hpv) genotypes have been carefully aligned. conserved primary and probable secondary structural features of the proteins have been identified. from the analysis of variations in the aligned positions, the most consistent evolutionary relationships have been reconstructed for each gene and for the genomes as a whole. the l1 gene encoding the major capsid protein and the e1 gene encoding a rep ... | 1999 | 12901616 |
| [clinical and experimental studies on condyloma acuminata]. | condyloma acuminata(ca) is one of sexually transmitted diseases(std) caused by human papillomavirus(hpv). we have done systemic study on the ca in recent years. analyzing the morbidity of ca in our std clinics showed that ca was in the first or second place of std. tissue specimens from ca were detected hpv dna by dot blot hybridization and pcr. the result showed pcr was more sensitive than dot blot hybridization. hpv dna were detected by pcr in tissue specimens of cervical squamous carcinoma, a ... | 2000 | 12903436 |
| [perianal bowen's disease: a case report and review of the literature]. | perianal bowen's disease is a uncommon, slow growing, intraepidermal squamous-cell carcinoma (carcinoma in situ) of the anal region and may be a precursor to squamous carcinoma of the anus. it is associated with cervical and vulvar intraepithelial neoplasia and have human papillomavirus as a common cause. both sexes and all races are affected, with the highest prevalence in patients aged 20 to 45 years. the symptoms of anal bowen's disease are unspecific and the clinical findings are uncharacter ... | 2003 | 12903534 |
| characterization of the transmembrane channel-like (tmc) gene family: functional clues from hearing loss and epidermodysplasia verruciformis. | mutations of tmc1 cause deafness in humans and mice. tmc1 and a related gene, tmc2, are the founding members of a novel gene family. here we describe six additional tmc paralogs (tmc3 to tmc8) in humans and mice, as well as homologs in other species. cdnas spanning the full length of the predicted open reading frames of the mammalian genes were cloned and sequenced. all are strongly predicted to encode proteins with 6 to 10 transmembrane domains and a novel conserved 120-amino-acid sequence that ... | 2003 | 12906855 |
| characterization of human papillomavirus infection, p53 and ki-67 expression in cervix cancer of mozambican women. | in this study, we aimed at evaluating the distribution of hpv types and the expression of p53 and ki-67 in cervix carcinomas of mozambican women. fourty-seven invasive carcinomas, 10 cin iii, and 10 normal cervix were studied. p53 and ki-67 expression was examined immunohistochemically. hpv infection and hpv types were detected by pcr (gp5+/bio-gp6+) and enzyme-immunoassay, respectively. expression of p53 and ki-67 and detection of hpv were as follows: normal cervix--0%, 10%, and 0%, respectivel ... | 2003 | 12908520 |
| obesity as a potential risk factor for adenocarcinomas and squamous cell carcinomas of the uterine cervix. | hormonal factors may play a more prominent role in cervical adenocarcinoma than squamous cell carcinoma. the authors evaluated whether obesity, which can influence hormone levels, was associated with adenocarcinoma and squamous cell carcinoma. | 2003 | 12910527 |
| serologic evidence of human papillomavirus 16 and 18 infections and risk of prostate cancer. | human papillomavirus (hpv) subtypes 16 and 18 are sexually transmitted and have been associated with an increased incidence of several anogenital tumors. although previous epidemiological studies have suggested that sexual behaviors such as an early age at first intercourse and larger numbers of sexual partners are also related to an increased risk of prostate cancer, seroepidemiological studies of these infectious agents in relation to prostate cancer have produced differing results. to further ... | 2003 | 12917208 |
| detection of human papillomavirus type 57 in the tissue of a plantar epidermoid cyst. | plantar epidermoid cysts with human papillomavirus (hpv) infection are not rare in japan. most of them show the cytopathic effect of hpv type 60 - homogeneous intracytoplasmic inclusion bodies. our case presented a plantar epidermoid cyst with vacuolation around the granular cell layer without any features of hpv type 60. in situ hybridization and polymerase chain reaction followed by dna sequencing analysis revealed an association with hpv type 57. | 2003 | 12920371 |
| human papillomavirus infections and oral tumors. | in the past 20 years, there has been an increasing interest in human papillomaviruses (hpv) because of their potential role in the pathogenesis of malignant tumors. in 1983, we published the first evidence that hpv might be involved in oral squamous cell carcinomas. the identification of morphological similarities between oral and cervical mucosa lead us to this original proposal. in a recent meta-analysis, hpv was indeed confirmed as an independent risk factor for oral carcinoma. to date, total ... | 2003 | 12920585 |
| [non-replicating recombinant vaccinia virus expressing hpv16 e6 and e7 proteins elicits anti-tumor immunity in mice]. | to investigate the anti-tumor immunity of the non-replicating recombinant vaccinia virus expressing hpv16 e6 and e7 proteins. | 2003 | 12921560 |
| comparison of the cd8+ t cell responses and antitumor effects generated by dna vaccine administered through gene gun, biojector, and syringe. | dna vaccines have emerged as an attractive approach for antigen-specific cancer immunotherapy. we have previously linked mycobacterium tuberculosis heat shock protein 70 (hsp70) to human papillomavirus type 16 (hpv-16) e7 in the context of a dna vaccine. vaccination with dna encoding e7/hsp70 has generated a dramatic increase of e7-specific cd8+ t cell precursors and a strong antitumor effect against e7-expressing tumor (tc-1) in vaccinated mice. the success of our strategy has led to two phases ... | 2003 | 12922140 |
| human papillomavirus and risk factors for cervical cancer in chennai, india: a case-control study. | to evaluate the role of human papillomavirus (hpv) and other risk factors in the aetiology of invasive cervical carcinoma (icc), we conducted a hospital-based case-control study in chennai, southern india. a total of 205 icc cases (including 12 adenocarcinomas) and 213 frequency age-matched control women were included. hpv dna in cervical cells was evaluated by means of a polymerase chain-reaction assay. odds ratios (ors) and corresponding 95% confidence intervals (cis) were computed by means of ... | 2003 | 12925967 |
| atypical squamous cells of undetermined significance in liquid-based cytologic specimens: results of reflex human papillomavirus testing and histologic follow-up in routine practice with comparison of interpretive and probabilistic reporting methods. | human papillomavirus (hpv) dna testing for high-risk types after papanicolaou (pap) smear interpretations of atypical squamous cells of undetermined significance (ascus) is a sensitive method for identifying women who harbor underlying high-grade squamous intraepithelial lesions (hsil). to the authors' knowledge, the application of hpv testing to ascus smears in routine practice with comparison of probabilistic and interpretive models of cytologic reporting has not been reported. | 2003 | 12925979 |
| is the human papillomavirus a mutual aetiological agent in oral and cervical squamous cell carcinoma? | oncogenic hpv-types are the most important risk factor in cervical ssc and have also been implicated in the aetiology of ossc. this evidence of infection at different anatomical sites suggests systemic susceptibility that implies that different expressions of disease should more or less correlate over long periods of time. | 2003 | 12926099 |
| human papillomavirus and dna ploidy in tonsillar cancer--correlation to prognosis. | human papillomavirus (hpv) is frequently observed in tonsillar cancer. here, tonsillar tumors were examined to see whether dna content varied depending on hpv status, and influenced clinical outcome. | 2003 | 12926119 |