Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| border disease virus isolated from a bovine fetus. | 2009 | 19395715 | |
| detection of border disease virus in fetuses, stillbirths, and newborn lambs from natural and experimental infections. | the purpose of the present study was to evaluate the use of enzyme-linked immunosorbent assay (elisa) antigen detection in blood or fetal fluids and reverse transcription polymerase chain reaction (rt-pcr) amplification in tissues for routine laboratory diagnosis of border disease virus (bdv) infection. samples from 67 fetuses, 6 stillbirths, and 11 lambs from 25 commercial flocks with suspicion of bdv abortion and 3 fetuses, 7 stillbirths, and 15 lambs obtained from an experimental infection wi ... | 2009 | 19407084 |
| antigenic differentiation of classical swine fever viruses in china by monoclonal antibodies. | the phylogenetic diversity of classical swine fever virus (csfv) in china has been extensively studied previously, with the report of the classification of chinese csfvs into four subgroups within two of the established genotypes, but the antigenic differences amongst chinese csf viruses still remain unknown. to address this issue, 21 csfv field strains isolated in china between 1996 and 2006 were grown in cell culture and characterized in comparison with two chinese reference strains: a virulen ... | 2009 | 19428750 |
| seroprevalence of antibodies against pestiviruses in small ruminants in the netherlands. | in this study, a serological survey was performed to determine the prevalence of pestivirus (bovine viral diarrhea virus (bvdv) and border disease virus (bdv)) infected small ruminants herds in the netherlands. after random selection of sheep farms, a sample size was determined to detect a 5% herd prevalence. 13 out of 29 farms were tested seropositive using an elisa which detects antibodies directed against the non structural protein 3 (ns3) of pestiviruses. this resulted in a seroprevalence fo ... | 2009 | 19480144 |
| first report of bvdv circulation in sheep in argentina. | pestiviruses are capable of infecting a wide range of animals within the order artyodactila. currently, the genus pestivirus includes bovine viral diarrhea virus 1 (bvdv-1) and 2 (bvdv-2), border disease virus (bdv), and classical swine fever virus (csfv). bvdv-1, bvdv-2 and bdv are able to cross species barrier to infect a wide range of hosts, whereas csfv is restricted to domestic pigs and wild boars. in argentina, 70% of cattle are seropositive to bvdv. although there were some serological st ... | 2009 | 19501923 |
| a short target real-time rt-pcr assay for detection of pestiviruses infecting cattle. | a rapid single step real-time duplex taqman rt-pcr was developed for detection of bovine viral diarrhoea virus (bvdv)-1, bvdv-2 and border disease virus (bdv). based on alignment of available and newly generated partial 5'-utr nucleotide sequences, one forward and two reverse primers were designed, which amplify a 104bp pcr product. two taqman probes labelled with different fluorochromes were designed to detect bvdv-1/bvdv-2 and bdvs, respectively. the assay was able to detect a selection of str ... | 2009 | 19523981 |
| mucosal lesions in a sheep infected with the border disease virus (bdv). | a 28-week-old sheep was presented at the animal hospital because of chronic emaciation, anemia and slight diarrhea. due to poor general condition and bad prognosis the animal was euthanized and submitted for postmortem investigation. multiple erosions and ulcerations were found in the dorsal region of the tongue, the pharynx, the hard palate, in the esophagus and the ruminal pillars. histologically, these lesions consisted of necrosuppurative inflammation. the animal was tested positive for pest ... | 2009 | 19653163 |
| influence of ns5a protein of classical swine fever virus (csfv) on csfv internal ribosome entry site-dependent translation. | an internal ribosome entry site (ires) present in the 5' untranslated region (utr) promotes translation of classical swine fever virus (csfv) genomes. using an in vitro system with monocistronic reporter rna containing the csfv 5'utr, this study found that csfv ns5a decreased csfv ires-mediated translation in a dose-dependent manner. deletion analysis showed that the region responsible for repressing csfv ires activity might cover aa 390-414, located in the c-terminal half of csfv ns5a. triple a ... | 2009 | 19710255 |
| antigenic and genetic characterisation of border disease viruses isolated from uk cattle. | available empirical data on the natural occurrence of ruminant pestiviruses has shown that in cattle, bovine viral diarrhoea virus (bvdv) is nearly exclusively found, whereas both border disease virus (bdv) and bvdv can be isolated from sheep. during routine genetic typing of pestivirus rna from uk cattle diagnosed as bvdv positive between 2006 and 2008, five samples that were classified as bdv positive yielded positive virus isolates in cell cultures. the samples originated from animals that ha ... | 2010 | 19781869 |
| generation of recombinant pestiviruses using a full-genome amplification strategy. | complete genome amplification of viral rna provides a new tool for the generation of modified viruses. we have recently reported a full-genome amplification strategy for recovery of pestiviruses (rasmussen et al., 2008). a full-length cdna amplicon corresponding to the border disease virus-gifhorn genome was generated by long rt-pcr and then rna transcripts derived from this amplicon were used to rescue infectious virus. here, we have now used this full-genome amplification strategy for efficien ... | 2010 | 19836906 |
| a novel combination of taqman rt-pcr and a suspension microarray assay for the detection and species identification of pestiviruses. | the genus pestivirus contains four recognized species: classical swine fever virus, border disease virus, bovine viral diarrhoea virus types 1 and 2. all are economically important and globally distributed but classical swine fever is the most serious, concerning losses and control measures. it affects both domestic pigs and wild boars. outbreaks of this disease in domestic pigs call for the most serious measures of disease control, including a stamping out policy in europe. since all the member ... | 2010 | 19854591 |
| bovine viral diarrhea virus infections in heterologous species. | infections with bovine viral diarrhea virus (bvdv) are not limited to cattle, but may be detected in various species in the mammalian order artiodactyla. despite epidemiological evidence of bvdv infections in species other than cattle, current knowledge regarding the impact of bvdv on heterologous species is incomplete. in heterologous hosts, bvdv infections with clinical signs analogous to those in cattle have been described and include disease of multiple organ systems, most notably the reprod ... | 2010 | 19883524 |
| detection and quantification of pestivirus in experimentally infected pregnant ewes and their progeny. | border disease virus (bdv) causes important reproductive losses, and eradication strategies focus on the identification and removal of persistently infected animals arising after in uterine infection. bdv infection dynamics were studied in 13 ewes experimentally infected with bdv-4 genotype at 3 phases of pregnancy [days 108 (group a), 76 (group b) and 55 (group c)] by quantification of viral rna in blood collected on days -1 to parturition using quantitative real-time rt-pcr (qrt-pcr). viral rn ... | 2009 | 19891772 |
| pestivirus infection in sheep and goats in west austria. | blood samples from 3112 sheep (185 flocks) and 1196 goats (163 flocks) from the western region of austria were tested for pestivirus-specific rna. in this area, communal alpine pasturing of sheep, cattle and goats is an important part of farming. the prevalence of sheep persistently-infected (pi) with pestivirus was 0.32% (10 animals) and the pi animals originated from five flocks (2.7% of those investigated). in goats, only one pi animal (0.08%) was detected. sequence analysis of the 5'-end unt ... | 2010 | 20042353 |
| prevalence and antigenic differences observed between bovine viral diarrhea virus subgenotypes isolated from cattle in australia and feedlots in the southwestern united states. | bovine viral diarrhea virus (bvdv) is divided into 2 different species within the pestivirus genus, bvdv type 1 (bvdv-1) and bvdv type 2 (bvdv-2). further phylogenetic analysis has revealed subgenotype groupings within the 2 types. thus far, 12 bvdv-1 subgenotypes (a-l) and 2 bvdv-2 subgenotypes (a and b) have been identified. the purpose of the current study was to determine the prevalence of bvdv subgenotypes in the united states and australia and to determine if there are detectable antigenic ... | 2010 | 20224075 |
| genetic variation of classical swine fever virus based on palindromic nucleotide substitutions, a genetic marker in the 5' untranslated region of rna. | forty-three strains of classical swine fever (hog cholera) virus (csfv) from outbreaks in pigs in europe, asia and america, two strains from commercial csfv modified live vaccines and a strain isolated from a diseased lamb from spain were subjected to analyses of nucleotide sequence variations in the 5' terminal region of the genome. these isolates were divided into three clusters, namely: csfv-1, csfv-2, and csfv-3, based on palindromic nucleotide substitutions in the 5' untranslated region (ut ... | 2008 | 20405435 |
| characteristics of the 5' untranslated region of wisent (bison bonasus) and reindeer (rangifer tarandus) pestivirus isolates. | the 5'-untranslated region (5'-utr) of the pestivirus strains isolated from wisent and reindeer suffering from severe diarrhoea in the duisburg zoo in germany were amplified by reverse transcription-polymerase chain reaction and sequenced for comparison with those of other pestiviruses from cattle, sheep, goats and swine. phylogenetic trees constructed from the primary nucleotide sequences of these strains demonstrated that the 5'-utr of both the wisent and reindeer isolates were identical and s ... | 2006 | 20429058 |
| experimental infection of pigs with border disease virus isolated from pyrenean chamois (rupicapra pyrenaica). | between 2001 and 2007, several outbreaks of disease associated with border disease virus (bdv) infection were reported in the central pyrenees (northeast spain) and were associated with a major reduction in chamois (rupicapra pyrenaica) populations. at the same time, wild boars (sus scrofa) from the same area were found to be seropositive to this pestivirus, without showing clinical signs. the present study examines the susceptibility of domestic swine and the course of the infection with a bdv ... | 2010 | 20453208 |
| neuropathologic study of border disease virus in naturally infected fetal and neonatal small ruminants and its association with apoptosis. | the present study describes the pathologic changes and cellular apoptosis in the central nervous system (cns) of fetal and neonatal small ruminants infected with border disease virus (bdv), as demonstrated by immunohistochemistry and in situ hybridization. abortions of ewes and goats were observed, as were births of lambs and kids with poor survival rates and nervous signs. lesions included cerebellar hypoplasia, porencephaly, hydranencephaly, and nonsuppurative meningoencephalomyelitis with hyp ... | 2011 | 20460448 |
| border disease virus seroprevalence correlates to antibodies in bulk-tank milk and reproductive performance of dairy sheep flocks. | there is a great need to establish effective tools to control border disease virus (bdv) in european dairy sheep flocks. hence, our main aim was to investigate the accuracy of analyzing anti-bdv antibodies in bulk-tank milk (btm) in detecting the real bdv seroprevalence in dairy sheep flocks. furthermore, the relevance of bdv to reproductive performance of dairy sheep flocks prompted us to search for the association between bdv seroprevalence and reproductive parameters. for these purposes, 34 f ... | 2010 | 20494152 |
| complete genomic sequence of a border disease virus isolated from pyrenean chamois. | this report describes the full-length genome sequence of the pestivirus strain h2121 which was recently isolated from pyrenean chamois and typed as border disease virus (bdv) genotype 4. comparison with full-length genomic sequences of the approved pestivirus species bovine viral diarrhea virus-1 (bvdv-1), bvdv-2, bdv, and classical swine fever virus, the tentative species represented by strain giraffe-1, as well as the atypical pestivirus strain th/04_khonkaen confirmed that the chamois pestivi ... | 2010 | 20638945 |
| haematology and serum chemistry of pyrenean chamois (rupicapra pyrenaica) naturally infected with a border disease virus. | in 2005 and 2006 an outbreak of disease associated with border disease virus (bdv) infection caused high mortality in the pyrenean chamois (rupicapra pyrenaica) in the catalan pyrenees (ne spain). the aim of this study was to determine values for different haematological and serum biochemical analytes in 32 free-ranging pyrenean chamois affected by the disease and to compare them with those obtained from healthy chamois. in the affected chamois red blood cell counts, haemoglobin concentrations, ... | 2011 | 20656309 |
| genetic and antigenic typing of border disease virus (bdv) isolates from italy reveals the existence of a novel bdv group. | border disease virus belongs to the pestivirus genus, within the family flaviviridae. genetic analysis of pestiviruses isolated from sheep in continental europe have led to the proposal that bdv isolates are segregated into at least seven clusters. in 2005 the molecular analysis of an italian caprine bdv strain provided evidence for the presence of an atypical pestivirus, which may represent the first member of a putative novel pestivirus sub-group. to further build on this study, ovine pestivir ... | 2011 | 20656426 |
| epidemiology of pestivirus infection in wild ungulates of the french south alps. | inter-species transmission is often incriminated in the epidemiology of pestivirus diseases. the purpose of this study was to investigate the prevalence of pestivirus in some mountain wild ungulates and to determine their role in pestivirus transmission, as mountain pastures are a place where cohabitations between wild and domestic ungulates are particularly high. between 2003 and 2007, a longitudinal epidemiological study was carried out on hunted ungulates in the french hautes-alpes department ... | 2011 | 20709472 |
| epidemiological study of pestiviruses in south american camelids in switzerland. | in the context of the ongoing eradication campaign for bovine viral diarrhea virus (bvdv) in cattle in switzerland, the role of south american camelids (sac) as a possible virus reservoir needed to be evaluated. | 2010 | 20726946 |
| border disease virus shedding and detection in naturally infected pyrenean chamois (rupicapra pyrenaica). | pyrenean chamois (rupicapra pyrenaica) populations of the central and eastern pyrenees have been affected by severe outbreaks associated with border disease virus (bdv) since 2001. eight pyrenean chamois (7 males and 1 female) from 1 to 8 years of age with clinical signs consistent with bdv infection were studied. at necropsy, whole blood, tissue samples (skin, brain, prescapular lymph node, thyroid gland, lung, liver, spleen, kidney, small intestine, bone marrow, and testicle), urine, and nasal ... | 2010 | 20807933 |
| retrospective study of pestivirus infection in pyrenean chamois (rupicapra pyrenaica) and other ungulates in the pyrenees (ne spain). | in 2001 a new pestivirus (family flaviviridae) was associated with an outbreak of a previously unreported disease in pyrenean chamois (rupicapra pyrenaica) in the pyrenees (ne spain). molecular characterization assigned this virus to the border disease virus (bdv) cluster, bdv-4 genotype. a retrospective study was performed in archived sera and spleen of 74 pyrenean chamois and in archived sera of 28 mouflon (ovis ammon), 56 red deer (cervus elaphus), 43 roe deer (capreolus capreolus) and 29 fal ... | 2010 | 20961710 |
| comparison of hematological and biochemical parameters in sheep naturally and persistently infected with a border disease virus. | in this study, we investigated the changes occurring in the activities of determining the biochemical and hematological parameters in persistently infected sheep with border disease virus (bdv) and control sheep. while cholesterol, aspartate aminotransferase, lactate dehydrogenase, high-density lipoprotein, and glucose parameters were found to be statistically different between control and bdv positive groups (p<0.01), total protein, alkaline phosphotase, creatine kinase, amylase, glucose, and h ... | 2010 | 21104124 |
| experimental infection of lambs with border disease virus isolated from a pyrenean chamois. | 2010 | 21257444 | |
| isolation and identification of a bovine viral diarrhea virus from sika deer in china. | bovine viral diarrhea virus (bvdv) infections continue to cause significantly losses in the deer population. better isolation and identification of bvdv from sika deer may contribute significantly to the development of prophylactic therapeutic, and diagnostic reagents as well as help in prevention and control of bvdv. however, isolation and identification of bvdv from sika deer is seldom reported in literature. in this study, we collected some samples according to clinical sign of bvdv to isolat ... | 2011 | 21352530 |
| a serological investigation of pestiviruses in sheep in eastern border of turkey. | all pestiviruses are important veterinary pathogens causing economic losses in cattle, sheep, and pigs. in this study, blood samples randomly collected from 465 sheep were analysed for the presence of antibodies to pestiviruses (bovine viral diarrhea virus, border disease virus) using an enzyme-linked immunosorbent assay in the province of van and their towns. the seroprevalance were estimated as 75.9% and 60.0-82.5% in the sampled animals and sampled towns, respectively. the results revealed th ... | 2011 | 21461731 |
| species characterization in the genus pestivirus according to palindromic nucleotide substitutions in the 5'-untranslated region. | the palindromic nucleotide substitutions (pns) at the three variable loci (v1, v2 and v3) in the 5'-untranslated region (utr) of the pestivirus genome have been considered for taxonomical segregation of the species, through the evaluation of 534 strains. on the basis of qualitative and quantitative secondary structure characteristics, species have been identified within the genus, determining genetic distances between species isolates, clarifying borderline and multirelated sequences, and charac ... | 2011 | 21514324 |
| experimental infection with chamois border disease virus causes long-lasting viraemia and disease in pyrenean chamois (rupicapra pyrenaica). | since 2001, severe outbreaks of disease associated with border disease virus (bdv) infection have been reported in the pyrenean chamois. the disease is characterized by variable degrees of cachexia, alopecia and neurological manifestations prior to death. the aim of this study was to investigate this disease under experimental conditions. to assess viral virulence, humoral immune response, dissemination and probable routes of transmission, seven chamois (five seronegative and two seropositive to ... | 2011 | 21775580 |
| epidemiological survey of border disease virus among sheep from northern districts of japan. | the first epidemiological survey of border disease virus (bdv) was undertaken in small ruminants in japan. ovine sera, collected from the northern prefectures of hokkaido, aomori and iwate, were examined for the presence of antibodies against bdv using the neutralization peroxidase-linked antibody test. twenty-nine (17.6%) of one hundred and sixty-five samples were seropositive for bdv. results were specific, excluding cross-reactions with bovine viral diarrhea virus (bvdv). only one sample (0.6 ... | 2011 | 21778667 |
| molecular detection of pestiviruses in aborted foetuses from provinces in northern turkey. | all pestiviruses are important veterinary pathogens causing economic losses in cattle, sheep and pigs. besides the important economical losses, pestiviruses may compromise the normal immune response to other pathogens and increase the severity of other infections in sheep. in this study, aborted foetuses (cattle and sheep) in either coastal or inland black sea region of turkey were surveyed for the presence of rna from pestiviruses (bovine viral diarrhoea virus (bvdv), border disease virus (bdv) ... | 2011 | 21842445 |
| a survey of antibodies to pestivirus in sheep in the republic of ireland. | : sera from 1,448 adult ewes in 91 flocks, representing all 26 counties in the republic of ireland, were examined for pestivirus antibodies using a commercially available elisa which detected igg1 antibody to border disease virus. eighty-one sheep (5.6%) in 42 flocks (46.0%) were antibody-positive. within infected flocks, the mean seroprevalence level was 11.4% with a range of 6.3% to 30.0%. the highest antibody prevalence was detected in sheep from central lowland counties of ireland. comparati ... | 2004 | 21851662 |
| Neurological diseases of ruminant livestock in Australia. IV: viral infections. | Most viral infections that affect the central nervous system of ruminants are exotic to Australia. As such, this review focuses on viruses of importance in Australian ruminants, including Akabane virus and the ruminant pestiviruses, bovine viral diarrhoea virus and border disease virus, as well as bluetongue virus. Each virus is discussed in terms of pathogenesis, clinical signs and diagnosis. | 2011 | 21864304 |
| dual infection of fetal and neonatal small ruminants with border disease virus and peste des petits ruminants virus (pprv): neuronal tropism of pprv as a novel finding. | dual infection of 26 fetal and neonatal small ruminants with border disease virus (bdv) and peste des petits ruminants virus (pprv) is reported. the animals included five aborted lamb fetuses, 19 neonatal lambs and two neonatal kids from flocks in regions of the black sea and the aegean region. bdv and pprv antigens were detected immunohistochemically in the brain, oral mucosa, intestine and lung of infected animals. reverse transcriptase-polymerase chain reaction was used to demonstrate pprv an ... | 2011 | 21899857 |
| development, optimization, and validation of a classical swine fever virus real-time reverse transcription polymerase chain reaction assay. | classical swine fever (csf) is an economically devastating disease of pigs. instrumental to the control of csf is a well-characterized assay that can deliver a rapid, accurate diagnosis prior to the onset of clinical signs. a real-time fluorogenic-probe hydrolysis (taqman) reverse transcription polymerase chain reaction (rt-pcr) for csf was developed by the united states department of agriculture (usda) at the plum island animal disease center (csf piadc assay) and evaluated for analytical and d ... | 2011 | 21908362 |
| Pestivirus and alphaherpesvirus infections in Swedish reindeer (Rangifer tarandus tarandus L.). | Herding semi-domesticated reindeer has economic and social value for Sami people in the northern territories of Fennoscandia. However, with the intensification of reindeer husbandry, interspecies transmission of pathogens between reindeer and domestic animals may become a problem, especially for countries such as Sweden, Norway, and Finland where pestivirus and alphaherpesvirus have been eradicated in domestic ruminants. This study, which included 1158 Swedish reindeer, showed relatively high pr ... | 2011 | 22078277 |
| genetic variation of border disease virus species strains. | the 5´-untranslated region of pestivirus strains isolated from domestic and wild animals were analysed to determine their taxonomic status according to nucleotide changes in the secondary genomic structure using the palindromic nucleotide substitutions (pns) method. a total of 131 isolates out of 536 pestivirus strains evaluated, were clustered as border disease virus (bdv) species. the bdv strains were further divided into at least 8 genotypes or subspecies. thirty-two isolates from small rumin ... | 2011 | 22194225 |
| epizootic of ovine congenital malformations associated with schmallenberg virus infection. | epizootic outbreaks of congenital malformations in sheep are rare and have, to the best of our knowledge, never been reported before in europe. this paper describes relevant preliminary findings from the first epizootic outbreak of ovine congenital malformations in the netherlands. between 25 november and 20 december 2011, congenital malformations in newborn lambs on sheep farms throughout the country were reported to the animal health service in deventer. subsequently, small ruminant veterinary ... | 2012 | 22393844 |
| comparative [corrected] codon usage between the three main viruses in pestivirus genus and their natural susceptible livestock. | classical swine fever virus, bovine viral diarrhea virus (bvdv), and border disease virus can cause serious livestock diseases. the relative synonymous codon usage value, the "effective number of codons" (enc), the ratio of k(s) value to k(a) value and the principle component analysis were employed to analyze the genetic characteristics of open reading frame (orf) and the four genes (the n(pro), erns, e1, e2 genes) of the three viruses and the relationship of codon usage pattern between each vir ... | 2012 | 22395914 |
| polymorphisms at mhc class ii drb1 exon 2 locus in pyrenean chamois (rupicapra pyrenaica pyrenaica). | chamois (rupicapra spp.) are mountain ungulates from southern and central europe and the near east. a newly reported border disease virus (bdv) has affected the easternmost populations of pyrenean chamois, leading to a dramatic population decrease that may drive to genetic variability loss. the major histocompatibility complex (mhc) is a sensitive marker for genetic variation of populations: polymorphism on the mhc genes is affected both by pathogens and population dynamics and it is ecologicall ... | 2012 | 22425496 |
| the first case of a bull persistently infected with border disease virus in new zealand. | poor reproductive performance was observed in 62 dairy heifers, with a pregnancy rate of 23% following 57 days mating with one 3-year-old and two 2-year old belted galloway bulls that were sourced from separate sheep and beef farms. | 2012 | 22550971 |
| production and characterization of monoclonal antibodies against a local isolate of classical swine fever virus. | monoclonal antibodies (mabs) against a classical swine fever virus (csfv; subgenogroup 1:1) isolate from assam, india were produced and characterized. four fusions of myeloma cells (sp2/0ag) were made with spleenocytes of 8-10 weeks old balb/c mice immunized with the viral antigen. several hybridoma clones secreting antibodies to the virus were obtained after four fusions, but five hybridoma clones secreting antibody specific to the virus could be stabilized. all the mabs belong to the igg2a iso ... | 2012 | 22822524 |
| short communication: performance of a blocking antibody elisa bulk-tank milk test for detection of dairy sheep flocks exposed to border disease virus. | the aim of this study was to investigate the test characteristics of a blocking antibody elisa applied to bulk-tank milk (btm) samples for the detection of dairy sheep flocks positive for antibodies to border disease virus. in 161 flocks recruited in 2009 and 2010, the antibody inhibition percentage (aip) in btm was compared with the prevalence estimate of antibody-positive ewes obtained from an age-representative sample of 45 milking ewes. a strong negative exponential relationship (r(2)=0.89) ... | 2012 | 22939791 |
| efficacy of an antiviral compound to inhibit replication of multiple pestivirus species. | pestiviruses are economically important pathogens of livestock. an aromatic cationic compound (db772) has previously been shown to inhibit bovine viral diarrhea virus (bvdv) type 1 in vitro at concentrations lacking cytotoxic side effects. the aim of this study was to determine the scope of antiviral activity of db772 among diverse pestiviruses. isolates of bvdv 2, border disease virus (bdv), hobi virus, pronghorn virus and bungowannah virus were tested for in vitro susceptibility to db772 by in ... | 2012 | 22985628 |
| surveillance of border disease in wild ungulates and an outbreak in pyrenean chamois (rupicapra pyrenaica pyrenaica) in andorra. | the principality of andorra is surrounded by areas in which pyrenean chamois (rupicapra pyrenaica pyrenaica) populations were severely affected by infection with border disease virus (bdv) which caused disease outbreaks between 2001 and 2009. nevertheless, the andorran chamois populations were not affected during this period. in light of the severe impact of bdv on several of the neighboring pyrenean chamois populations, we monitored local andorran populations in an effort to detect pestivirus a ... | 2012 | 23060503 |
| development and evaluation of indirect enzyme-linked immunosorbent assay for a screening test to detect antibodies against classical swine fever virus. | an indirect enzyme-linked immunosorbent assay (elisa) was developed for a screening test to detect antibodies against classical swine fever virus (csfv). viral glycoproteins, which were purified from swine kidney cells infected with csfv ald/a76 strain by the immunoaffinity purification using monoclonal antibody against e2 protein, were adsorbed on a microtiter plate as the antigen for the antibody detection. each antibody titer of serum sample was expressed as a sample per positive value calcul ... | 2012 | 23094583 |
| two different epidemiological scenarios of border disease in the populations of pyrenean chamois (rupicapra p. pyrenaica) after the first disease outbreaks. | since 2001 several outbreaks of a new disease associated with border disease virus (bdv) infection have caused important declines in pyrenean chamois (rupicapra pyrenaica) populations in the pyrenees. the goal of this study was to analyze the post-outbreak bdv epidemiology in the first two areas affected by disease with the aim to establish if the infection has become endemic. we also investigated if bdv infected wild and domestic ruminants sharing habitat with chamois. unexpectedly, we found di ... | 2012 | 23251417 |
| experimental infection of pregnant pyrenean chamois (rupicapra pyrenaica) with border disease virus subtype 4. | border disease virus (bdv) causes high mortality in pyrenean chamois (rupicapra pyrenaica) on the french and spanish sides of the pyrenees mountains. we investigated the pathology induced by bdv in pregnant chamois via experimental infection. three females were inoculated during the second third of pregnancy with a bdv-4 subgroup strain isolated from a wild pyrenean chamois during an acute epizootic. a fourth pregnant chamois and one nonpregnant ewe were kept as negative controls. animals were m ... | 2013 | 23307372 |
| hobi-like viruses: an emerging group of pestiviruses. | the genus pestivirus is composed of 4 important pathogens of livestock: bovine viral diarrhea virus 1 and 2 (bvdv-1 and bvdv-2), classical swine fever virus (csfv), and border disease virus of sheep (bdv). bvdv are major pathogens of cattle, and infection results in significant economic loss worldwide. a new putative pestivirus species, tentatively called "hobi-like," "bvdv-3," or "atypical pestiviruses," was first identified in europe in fetal bovine serum (fbs) imported from brazil. hobi-like ... | 2013 | 23345268 |
| infection of cattle with border disease virus by sheep on communal alpine pastures. | the purpose of this study was to investigate whether sheep grazing communal alpine pastures with cattle can transmit border disease virus (bdv) to cattle. a total of 1170 sheep and 923 cattle were tested for bdv using rt-pcr (sheep) and for pestivirus antibodies using an elisa (cattle), respectively, before being moved to one of 4 pastures (a, b, c and d). eight sheep from pasture c were viraemic. 396 of 923 cattle examined before the pasture season were seronegative. the latter were re-examined ... | 2013 | 23385070 |
| detection of border disease virus (bdv) in goat herds suffering diarrhea in eastern china. | border disease virus (bdv) is an important pathogen in sheep and goat production. neither epidemiological investigation nor any reports of bdv infection was available in china. during jan to apr, 2012, several herd goats in anhui and jiangsu provinces in eastern china suffered unremitting diarrhea, with morbidity and mortality of about 28-37% and 10-15%, respectively. in the present study, sera and tissue samples from diseased goats of four farms were taken for bdv detection, isolation and ident ... | 2013 | 23497341 |
| genetic characterization of a border disease virus isolate originating from slovakia. | in this study, a major part of genome of the pestivirus isolate 297 from slovakia, comprising the 7195 nt-long 5΄-utr-ns3 region was sequenced and analyzed. conserved cleavage sites between individual viral proteins of this region were determined and the number of amino acids of respective proteins was estimated as follows: 168 for npro, 100 for c, 227 for erns, 195 for e1, 373 for e2, 70 for p7, 453 for ns2, and 683 for ns3. based on sequence and phylogenetic analysis of 5΄-utr, npro, and e2 th ... | 2013 | 23530820 |
| identification and characterization of mimotopes of classical swine fever virus e2 glycoprotein using specific anti-e2 monoclonal antibodies. | classical swine fever virus (csfv) shares high nucleic acid and amino acid sequence homology with the other members of the pestivirus genus, namely bovine viral diarrhea virus and border disease virus. all three viruses are able to infect swine and generate cross reactive antibodies, which is problematic during differential diagnosis for classical swine fever (csf). toward the development of a new generation of csf specific diagnostic tools, monoclonal antibodies specific for csfv were mapped us ... | 2013 | 23583685 |
| investigation of border disease and bovine virus diarrhoea in sheep from 76 mixed cattle and sheep farms in eastern switzerland. | the purpose of this study was to examine the occurrence of sheep persistently infected with border disease virus (bdv) on 76 mixed cattle and sheep farms and whether seroconversion to bdv infection occurred in cattle of these farms. seroprevalence of bdv and bovine viral disease virus (bvdv) infection in sheep was also investigated. quantitative rt-pcr for pestivirus detection and an elisa to detect pestivirus antibodies were used in 2'384 and 2'291 ovine blood samples, respectively. another 27 ... | 2013 | 23644292 |
| numerical taxonomy of the genus pestivirus: new software for genotyping based on the palindromic nucleotide substitutions method. | the genus pestivirus from the family flaviviridae is represented by four established species; bovine viral diarrhea virus 1 (bvdv-1); bovine viral diarrhea virus 2 (bvdv-2); border disease virus (bdv); and classical swine fever virus (csfv); as well a tentative species from a giraffe. the palindromic nucleotide substitutions (pns) in the 5' untranslated region (utr) of pestivirus rna has been described as a new, simple and practical method for genotyping. new software is described, also named pn ... | 2013 | 23684846 |
| in vitro neutralization of hobi-like viruses by antibodies in serum of cattle immunized with inactivated or modified live vaccines of bovine viral diarrhea viruses 1 and 2. | hobi-like viruses are an emerging species of pestiviruses with genetic and antigenic similarities to bovine viral diarrhea viruses 1 and 2 (bvdv1 and bvdv2). vaccines for hobi-like viruses are not yet available. however, both modified live virus (mlv) and killed virus (kv) vaccines against bvdv are widely used worldwide. this study evaluated the cross reactive antibody response against hobi-like pestiviruses in sera of cattle immunized with bvdv1 and bvdv2 vaccines. groups "kv" and "mlv", with 2 ... | 2013 | 23764273 |
| preliminary mapping of non-conserved epitopes on envelope glycoprotein e2 of bovine viral diarrhea virus type 1 and 2. | bovine viral diarrhea virus (bvdv) belongs together with classical swine fever virus (csfv) and border disease virus (bdv) to the genus pestivirus in the flaviviridae family. bvdv has been subdivided into two different species, bvdv1 and bvdv2 based on phylogenetic analysis. subsequent characterization of both strains revealed major antigenic differences. because the envelope glycoprotein e2 is the most immunodominant protein for all pestiviruses, the present study focused on epitope mapping by ... | 2013 | 23838147 |
| approved and experimental countermeasures against pestiviral diseases: bovine viral diarrhea, classical swine fever and border disease. | the pestiviruses, bovine viral diarrhea virus (bvdv), classical swine fever (csfv) and border disease virus, are important livestock pathogens in many countries, but current vaccines do not completely prevent the spread of infection. control of pestiviral diseases is especially difficult due to the constant viremia and viral shedding of persistently infected (pi) animals, which must be identified and eliminated to prevent disease transmission. existing vaccines are limited by the delay between v ... | 2013 | 23928259 |
| ostertagiosis causes illthrift and death in older cattle. | ostertagiosis diagnosed in a six-year-old suckler cow. congenital ear deformities seen in several ayrshire-cross calves. border disease virus infection associated with numerous neonatal lamb deaths. coccidiosis, pcv-2 and hepatosis dietetica implicated in piglet deaths. spinal aspergillosis suspected in pheasant poults with lameness and paralysis. these are among matters discussed in the disease surveillance report for july from sac consulting: veterinary services (sac c vs). | 2013 | 24097882 |
| natural border disease virus infection in feedlot lambs. | 2014 | 24106248 | |
| genome sequence of border disease virus strain jsls12-01, isolated from sheep in china. | border disease virus (bdv) is a recognized virus in the genus pestivirus and causes border disease (bd) in sheep and goats. here, a novel bdv strain, jsls12-01, was identified from sheep in jiangsu province, china. the complete coding sequence (cds) was finished, which provides a better understanding of the molecular evolution of bdv isolates. | 2013 | 24201189 |
| serosurvey on schmallenberg virus and selected ovine reproductive pathogens in culled ewes from southern spain. | after the first case of schmallenberg virus (sbv) was reported in southern spain (march 2012), a retrospective serological study was carried out in extensive sheep flocks from nearby areas to assess the history of exposure to sbv and other selected ovine reproductive pathogens (chlamydophila abortus, coxiella burnetii, border disease virus 'bdv', toxoplasma gondii and neospora caninum). secondly, the presence of antibodies was investigated in meat juice samples against selected pathogens to vali ... | 2014 | 24219149 |
| identification of a porcine pestivirus as a border disease virus from naturally infected pigs in spain. | 2014 | 24285010 | |
| sheep persistently infected with border disease readily transmit virus to calves seronegative to bvd virus. | bovine viral diarrhea- and border disease viruses of sheep belong to the highly diverse genus pestivirus of the flaviviridae. ruminant pestiviruses may infect a wide range of domestic and wild cloven-hooved mammals (artiodactyla). due to its economic importance, programs to eradicate bovine viral diarrhea are a high priority in the cattle industry. by contrast, border disease is not a target of eradication, although the border disease virus is known to be capable of also infecting cattle. in thi ... | 2014 | 24315041 |
| n pro of bungowannah virus exhibits the same antagonistic function in the ifn induction pathway than that of other classical pestiviruses. | bungowannah virus is the most divergent atypical pestivirus that had been detected up to now, and does not fit into any of the four approved species: bovine viral diarrhea virus type 1 (bvdv-1) and type 2 (bvdv-2), classical swine fever virus (csfv) and border disease virus (bdv). however, the presence of n(pro) and e(rns) coding regions, which are unique to pestiviruses, provides clear evidence of a pestivirus. nevertheless, the amino acid identity of bungowannah virus n(pro) and bvdv-1 n(pro) ... | 2014 | 24398226 |
| detection of border disease virus (bdv) genotype 3 in italian goat herds. | in january 2011, cases of abortion, stillbirth and weak live kids were reported in two goat herds in northern italy. samples from 18 kids found dead, 12 fetuses, and two stillborn kids were analyzed for pestivirus antigen using an elisa kit and a border disease virus (bdv)-specific rt-pcr. positive results were obtained in six kids and one fetus. phylogenetic analysis based on 225 bp of the 5'utr fragment of the bdv genome from positive samples showed that the goats were infected with bdv genoty ... | 2014 | 24418604 |
| complete genome sequence of border disease virus genotype 3 strain gifhorn. | the complete genome sequence of the genotype 3 border disease virus strain gifhorn has been determined; this strain was originally isolated from pigs. this represents the consensus sequence for the virus used to produce the bacterial artificial chromosome (bac) cdna clone pbelogif3, which yields a virus that is severely attenuated in cell culture. | 2014 | 24435861 |
| border disease virus: time to take more notice? | 2014 | 24443466 | |
| pathogens of zoonotic and biological importance in roe deer (capreolus capreolus): seroprevalence in an agro-system population in france. | antibody prevalence for several infectious and parasitic diseases in a population of roe deer (capreolus capreolus) inhabiting a mixed agricultural landscape (south of france) has been analyzed. serological analyses with elisa in 245 animals captured from 2008 to 2012 has been performed. we found a high prevalence of toxoplasma gondii (46.4%), chlamydophila abortus (17.27%) and coxiella burnetii (11.26%) compared to other studies in europe. seroprevalence varied strongly among years for t. gondi ... | 2014 | 24576494 |
| identification and epidemiology of a rare hobi-like pestivirus strain in bangladesh. | the genus pestivirus of the family flaviviridae consists of four recognized species: bovine viral diarrhoea virus 1 (bvdv-1), bovine viral diarrhoea virus 2 (bvdv-2), classical swine fever virus and border disease virus. a new putative pestivirus species tentatively named as either 'hobi-like pestivirus' or bvdv-3 has recently been identified in brazil, italy and thailand. despite reports of serological evidence of bvdv in bangladesh, the types of the virus circulating in cattle have not been id ... | 2014 | 24650238 |
| clinical presentation resembling mucosal disease associated with 'hobi'-like pestivirus in a field outbreak. | the genus pestivirus of the family flaviviridae consists of four recognized species: bovine viral diarrhoea virus 1 (bvdv-1), bovine viral diarrhoea virus 2 (bvdv-2), classical swine fever virus (csfv) and border disease virus (bdv). recently, atypical pestiviruses ('hobi'-like pestiviruses) were identified in batches of contaminated foetal calf serum and in naturally infected cattle with and without clinical symptoms. here, we describe the first report of a mucosal disease-like clinical present ... | 2016 | 24735072 |
| first isolation of border disease virus in japan is from a pig farm with no ruminants. | the first isolation of border disease virus (bdv) in japan was from a pig farm of the farrow-to-finishing type that kept no small ruminants or cattle. the infection was detected in the course of sero-surveillance for classical swine fever virus (csfv) in japan. the infected pigs had no clinical symptoms of csfv or other disease; nevertheless, a high sero-positive rate of 58.5% was identified. a persistently infected pig with the bdv was found and suspected to be the cause of sero-prevalence in t ... | 2014 | 24735918 |
| molecular characterization of border disease virus strain aveyron. | for the pestivirus border disease virus (bdv) at least seven major genotypes have been described (bdv-1-bdv-7). so far, complete genomic sequences have been reported for four bdv genotypes (bdv-1-bdv-4). in this study we report the entire genomic sequence of the noncytopathogenic (ncp) bdv-5 reference strain aveyron. the viral genome encompasses 12,284 nucleotides (nt) and contains one large open reading frame (11,700 nt) flanked by a 370 nt long 5'-untranslated region (utr) and a 214 nt long 3' ... | 2014 | 24742950 |
| molecular, biological, and antigenic characterization of a border disease virus isolated from a pig during classical swine fever surveillance in japan. | in the current study, molecular, biological, and antigenic analyses were performed to characterize border disease virus (bdv) strain fnk2012-1 isolated from a pig in 2012 in japan. the complete genome comprises 12,327 nucleotides (nt), including a large open reading frame of 11,685 nt. phylogenetic analysis revealed that fnk2012-1 was clustered into bdv genotype 1 with ovine strains. fnk2012-1 grew in porcine, bovine, and ovine primary cells and cell lines, but grew better in bovine and ovine ce ... | 2014 | 25027496 |
| neospora caninum infection as a cause of reproductive failure in a sheep flock. | neospora caninum has been detected only sporadically in cases of ovine abortion, and it has therefore traditionally been considered as an unimportant parasite in small ruminants. this study was carried out with the aim of identifying the pathogen causing serious reproductive problems on a commercial sheep farm. sera from all rams and ewes tested negative for antibodies against border disease virus, schmallenberg virus and coxiella burnetii, and infections by these agents were therefore ruled out ... | 2014 | 25158756 |
| pestiviruses. | pestiviruses cause economically important diseases among domestic ruminants and pigs, but they may also infect a wide spectrum of wild species of even-toed ungulates (artiodactyla). bovine viral diarrhea virus (bvdv) and border disease virus of sheep infect their hosts either transiently or persistently. cellular and humoral immunotolerance to the infecting strain is a unique feature of persistent infection (pi) by ruminant pestiviruses. persistence, caused by transplacental infection early in f ... | 2014 | 25384138 |
| pathogenicity of border disease virus fnk2012-1 strain isolated from a pig in the natural host, sheep. | a first isolation of border disease virus (bdv) in japan was from a pig on a farm without keeping any ruminants. our previous study showed that this bdv, termed the fnk2012-1 strain, replicated inefficiently in swine-derived cells compared with those of ruminant origin. pigs inoculated with this virus showed neither clinical symptoms nor viremia. in this study, we evaluated the pathogenicity of the fnk2012-1 strain in sheep, its natural host. the inoculated sheep showed clinical symptoms and tra ... | 2015 | 25391535 |
| genetic characterization of border disease virus (bdv) isolates from small ruminants in italy. | border disease virus (bdv) belongs to the pestivirus genus in the family flaviviridae. genetic analyses of pestiviruses that have been isolated from sheep and goat have led to the proposal that bdv isolates can be phylogenetically segregated into at least seven clusters, subtypes bdv-1 to bdv-7. in order to investigate the genetic heterogeneity of small ruminant pestivirus isolates in italy, a selection of 5'-utr sequences from isolates that were collected from clinical specimens between 2002 an ... | 2015 | 25636968 |
| insulated isothermal reverse transcriptase pcr (iirt-pcr) for rapid and sensitive detection of classical swine fever virus. | classical swine fever (csf) is an oie-listed disease that can have a severe impact on the swine industry. user-friendly, sensitive, rapid diagnostic tests that utilize low-cost field-deployable instruments for csf diagnosis can be useful for disease surveillance and outbreak monitoring. in this study, we describe validation of a new probe-based insulated isothermal reverse transcriptase pcr (iirt-pcr) assay for rapid detection of classical swine fever virus (csfv) on a compact, user-friendly dev ... | 2016 | 25644051 |
| erratum to: genetic characterization of border disease virus (bdv) isolates from small ruminants in italy. | 2015 | 25792189 | |
| increased expressions of adamts-13, neuronal nitric oxide synthase, and neurofilament correlate with severity of neuropathology in border disease virus-infected small ruminants. | border disease (bd), caused by pestivirus from the family flaviviridae, leads to serious reproductive losses and brain anomalies such as hydranencephaly and cerebellar hypoplasia in aborted fetuses and neonatal lambs. in this report it is aimed to investigate the expression of neuronal nitric oxide synthase (nnos), a disintegrin and metalloprotease with thrombospondin type i repeats-13 (adamts-13), and neurofilament (nf) in the brain tissue in small ruminants infected with border disease virus ( ... | 2015 | 25799514 |
| short communication: transmission of border disease virus to seronegative cows inseminated with infected semen. | the goal of this study was to investigate the transmissibility of border disease (bd) virus to seronegative cows via artificial insemination with cryopreserved semen from a bull persistently infected with bd virus. five pestivirus naive cows were inseminated with bd virus-infected semen. blood was collected for detection of pestivirus antibody by means of an elisa on day 0 (day of insemination) and then every 7 days until day 56, at which time a serum neutralisation test (snt) for differentiatio ... | 2015 | 25863814 |
| enos and inos trigger apoptosis in the brains of sheep and goats naturally infected with the border disease virus. | in this study, apoptotic and anti-apoptotic mechanisms and if present, which pathway to trigger the apoptosis in the brains of border disease virus (bdv) infected lambs (n=10) and goat kids (n=5) were investigated. briefly, apoptotic (caspase 3, caspase 9) and anti-apoptotic markers (bcl-2), cytokine response (tnf-α, inf-γ), reactive gliosis and myelin loss were examined. enos, inos, caspase 9, caspase 3 and gfap expressions were higher in bdv infected tissues compared to control animals (6 kids ... | 2015 | 25882134 |
| transmission of border disease virus from a persistently infected calf to seronegative heifers in early pregnancy. | this study describes the transmission of border disease virus (bdv) from a persistently infected calf to seronegative heifers in early pregnancy, resulting in persistently infected fetuses. on day 50 of pregnancy (= day 0 of the infection phase), six heifers were co-housed in a free stall with a bull calf persistently infected with bdv (pi bvd) for 60 days. the heifers underwent daily clinical examination, and blood samples were collected regularly for detection of pestiviral rna and anti-pestiv ... | 2015 | 25889936 |
| characterization of one sheep border disease virus in china. | border disease virus (bdv) causes border disease (bd) affecting mainly sheep and goats worldwide. bdv in goat herds suffering diarrhea was recently reported in china, however, infection in sheep was undetermined. here, bdv infections of sheep herds in jiangsu, china were screened; a bdv strain was isolated and identified from the sheep flocks in china. the genomic characteristics and pathogenesis of this new isolate were studied. | 2015 | 25890073 |
| emerging pestiviruses infecting domestic and wildlife hosts. | until the early 1990 s there were just three recognized species in the pestivirus genus, bovine viral diarrhea virus (bvdv), border disease virus (bdv) and classical swine fever virus (csfv). subsequently bvdv were divided into two different species, bvdv1 and bvdv2 and four additional putative pestivirus species have been identified, based on phylogenetic analysis. the four putative pestivirus specices, listed in chronological order of published reports, are giraffe (isolated from one of severa ... | 2015 | 26050572 |
| the two sides of border disease in pyrenean chamois (rupicapra pyrenaica): silent persistence and population collapse. | in 2001, border disease virus (bdv) was identified as the cause of a previously unreported disease in pyrenean chamois (rupicapra pyrenaica) in spain. since then, the disease has caused a dramatic decrease, and in some cases collapse, of chamois populations and has expanded to nearly the entire distribution area in the pyrenees. chamois bdv was characterized as bdv-4 genotype and experimental studies confirmed that it was the primary agent of the disease. the infection has become endemic in the ... | 2015 | 26050575 |
| the molecular biology of pestiviruses. | pestiviruses are among the economically most important pathogens of livestock. the biology of these viruses is characterized by unique and interesting features that are both crucial for their success as pathogens and challenging from a scientific point of view. elucidation of these features at the molecular level has made striking progress during recent years. the analyses revealed that major aspects of pestivirus biology show significant similarity to the biology of human hepatitis c virus (hcv ... | 2015 | 26111586 |
| chinese border disease virus strain jsls12-01 infects piglets and down-regulates the antibody responses of classical swine fever virus c strain vaccination. | during 2012 and 2013, several border disease virus (bdv) strains were identified from chinese goat and sheep herds. at the same time, pigs from the same areas were found to be seropositive to bdv by elisa, without showing clinical signs (unpublished data). to examine the susceptibility of pigs to the chinese bdv strains, bdv isolate jsls12-01, isolated from naturally infected sheep, was used to infect pigs. antibody responses, viremia, clinical signs and pathological changes of the infected anim ... | 2015 | 26117151 |
| ligand-responsive rna mechanical switches. | ligand-responsive rna mechanical switches represent a new class of simple switching modules that adopt well-defined ligand-free and bound conformational states, distinguishing them from metabolite-sensing riboswitches. initially discovered in the internal ribosome entry site (ires) of hepatitis c virus (hcv), these rna switch motifs were found in the genome of diverse other viruses. although large variations are seen in sequence and local secondary structure of the switches, their function in vi ... | 2015 | 26158858 |
| antigenic and molecular characterisation of border disease virus associated with high mortality in lambs in spain. | border disease virus (bdv) causes congenital disorders in sheep and results in severe, but underestimated, economic losses worldwide. however, information about bdv strains affecting several ruminants worldwide is scarce. therefore, antigenic and genetic classification of isolates from different geographical regions is important to enhance the knowledge of the epidemiology of bdv. | 2015 | 26392884 |
| genetic diversity of brazilian bovine pestiviruses detected between 1995 and 2014. | pestivirus infections in ruminants result in significant economic losses worldwide. the aetiological agents are three species from the genus pestivirus, family flaviviridae, including bovine viral diarrhoea virus type 1 (bvdv-1), bvdv-2, border disease virus (bdv), and an atypical pestivirus named hobi-like pestivirus. in this study, eighty-nine pestivirus isolates that were collected in brazil between 1995 and 2014 and that originated from either cattle, fetal bovine serum (fbs) or as cell cult ... | 2017 | 26415862 |
| pestivirus infection in reindeer (rangifer tarandus). | reindeer species (rangifer tarandus, linnaeus, 1758) includes wild and semi-domesticated ruminants belonging to capreaolinae subfamily of cervidae family reared in eurasia (reindeer subspecies) and north america (caribou subspecies). herding of reindeer has a great historical, socio-economic and ecological importance, especially to indigenous ethnic minorities. infectious disease threats may therefore impact not solely the animal population driving it to further extinction and irreversible alter ... | 2015 | 26579094 |
| a new genotype of border disease virus with implications for molecular diagnostics. | border disease virus (bdv) is a (+) single-stranded rna pestivirus affecting mainly sheep and goats worldwide. genetic typing of bdv has led to the identification of at least seven major genotypes. this study reports the detection of a bdv strain from a goat in northwestern italy during routine investigations. sequence analysis revealed mutations in the 5'-utr of the virus with implications for bdv molecular diagnostics. moreover, subsequent phylogenetic analysis based on the combined 5'-utr and ... | 2016 | 26597186 |
| border disease virus: an exceptional driver of chamois populations among other threats. | though it is accepted that emerging infectious diseases are a threat to planet biodiversity, little information exists about their role as drivers of species extinction. populations are also affected by natural catastrophes and other pathogens, making it difficult to estimate the particular impact of emerging infectious diseases. border disease virus genogroup 4 (bdv-4) caused a previously unreported decrease in populations of pyrenean chamois (rupicapra pyrenaica pyrenaica) in spain. using a po ... | 2015 | 26733943 |
| identification and molecular characterization of border disease virus (bdv) from sheep in india. | pestiviruses isolated from sheep and goats in india thus far have been bovine viral diarrhoea virus 1 (bvdv-1) or bvdv-2. during routine genetic typing of pestiviruses in the years 2009-10, border disease virus (bdv) was detected in eight indian sheep of a flock showing clinical signs of bd by real time rt-pcr. all the samples yielded positive virus isolates in cell culture but were found negative by a bvdv antigen elisa. a representative bdv isolate was characterized at genetic and antigenic le ... | 2016 | 26851587 |