Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| fusarium infections in immunocompromised patients: case reports and literature review. | five cases are reported of fusarium infection in patients with aplasia following chemotherapy of leukemia. the clinical signs, diagnosis and course of the infection during treatment are outlined and discussed in conjunction with the characteristics of other cases already reported in the literature. sixty-three cases of fusarium infection have been reported in immunocompromised patients, 44 cases since 1985. these included patients with hematological malignancies (58 cases), especially acute leuk ... | 1994 | 8013489 |
| pseudothionin-st1, a potato peptide active against potato pathogens. | a 5-kda polypeptide, pseudothionin solanum tuberosum 1 (pth-st1), which was active against clavibacter michiganensis subspecies sepedonicus, a bacterial pathogen of potatoes, has been purified from the buffer-insoluble fraction of potato tubers by salt extraction and hpcl. pth-st1 was also active against other potato pathogens tested (pseudomonas solanacearum and fusarium solani). the n-terminal amino acid sequence of this peptide was identical (except for a n/h substitution at position 2) to th ... | 1994 | 8033886 |
| purification and characterization of fus si3596*, a 65 kd allergen of fusarium solani. | a component of fusarium solani (f. solani), identified as the major allergen, fus si3596* was purified to homogeneity from culture filtrate (cf) by means of anion-exchange column chromatography, gel filtration and fplc. the homogeneity of fus si3596* was assessed by ief, page, sds-page (non-reducing), immunoblot and hplc. fus si3596* was isolated as a glycoprotein of mw 65 kd and pi 3.6. the ige elisa-inhibition assay after periodate treatment of the fraction showed a lower ige binding capacity ... | 1994 | 8035781 |
| endogenous endophthalmitis due to fusarium: case report and review. | endogenous fungal endophthalmitis is being increasingly recognized in susceptible individuals. we report a case of endogenous endophthalmitis due to fusarium solani that occurred as the sole clinical manifestation of fungal disease in an immunocompromised host. four previously reported cases of endogenous fusarial endophthalmitis are also reviewed. two of these patients had no underlying disease and presented with isolated endophthalmitis, while two other patients had acute leukemia and presente ... | 1994 | 8038313 |
| cutinase gene disruption in fusarium solani f sp pisi decreases its virulence on pea. | fusarium solani f sp pisi (nectria haematococca) isolate 77-2-3 with one cutinase gene produced 10 to 20% of the cutinase produced by isolate t-8 that has multiple cutinase genes, whereas cutinase gene-disrupted mutant 77-102 of isolate 77-2-3 did not produce cutinase. on the surface of pea stem segments, lesion formation was most frequent and most severe with t-8, less frequent and less severe with 77-2-3, and much less frequent and much milder with the gene-disrupted mutant. microscopic examin ... | 1994 | 8069105 |
| in vitro sensitivity of medically significant fusarium species to various antimycotics. | sixteen isolates belonging to fusarium chlamydosporum (n = 4), fusarium equiseti (n = 1), fusarium moniliforme (n = 2), fusarium oxysporum (n = 3), fusarium proliferatum (n = 1), and fusarium solani (n = 5) were tested against amphotericin b, 5-fluorocytosine, fluconazole, itraconazole, ketoconazole, jai-amphotericin b (water-soluble compound), hamycin and amphotericin b combined with 5-fluorocytosine, using antibiotic medium m3, high-resolution broth (ph 7.1), sabouraud's dextrose, and yeast-ni ... | 1994 | 8082411 |
| studies on fusarium solani. cross-reactivity among fusarium species. | allergenic cross-reactivity among three fusarium species (f. solani, f. equiseti, and f. moniliforme) was determined by crossed immunoelectrophoresis (cie), elisa inhibition, and immunoprint-inhibition analysis. inhibiting immunoprint was used to assess the cross-reactivity of individual allergenic proteins in culture filtrate (cf), separated on sds-page. dose-response inhibition of band 14 kda in immunoprints of f. solani cf by antigens of f. solani cf, f. equiseti cf, and f. moniliforme cf dem ... | 1994 | 8092429 |
| a novel pathogen- and wound-inducible tobacco (nicotiana tabacum) protein with antifungal activity. | a novel pathogen- and wound-inducible antifungal protein of 20 kd was purified from tobacco (nicotiana tabacum) samsun nn leaves inoculated with tobacco mosaic virus (tmv). the protein, designated cbp20, was purified by chitin-affinity chromatography and gel filtration. in vitro assays demonstrated that cbp20 exhibits antifungal activity toward trichoderma viride and fusarium solani by causing lysis of the germ tubes and/or growth inhibition. in addition it was shown that cbp20 acts synergistica ... | 1994 | 8115541 |
| identification of regulatory elements in the cutinase promoter from fusarium solani f. sp. pisi (nectria haematococca). | the cutinase gene from fusarium solani f. sp. pisi (nectria haematococca) is induced upon contact with the plant cuticular polymer, cutin, by the unique hydroxy fatty acid monomers released by cutinase carried by virulent strains of the fungus, and this gene is also catabolite-repressed by glucose. functional elements of the cutinase promoter were studied in vivo by transforming f. solani pisi with fusions of 5'-flanking regions of the cutinase gene and the gene encoding chloramphenicol acetyltr ... | 1994 | 8132657 |
| antifungal activities of crude extracts of mitracarpus villosus (rubiaceae). | extracts of mitracarpus villosus leaves and inflorescences were investigated individually for in vitro antifungal activities by agar-diffusion and tube-dilution techniques. ethanolic extracts produced definite antifungal activities against trichophyton rubrum, microsporum gypseum, candida albicans, aspergillus niger and fusarium solani. the aqueous extracts and the glycerol vehicle control did not inhibit any of the fungi tested. the zones of inhibition produced by the ethanol extracts ranged fr ... | 1993 | 8133654 |
| fusarium solani: immunochemical characterization of allergens. | allergenic components of the fungus fusarium solani were isolated using (nh4)2so4 precipitation and ion-exchange column chromatography. the allergenicity of fractions was studied by enzyme-linked immunosorbent assay and radioallergosorbent test inhibition techniques. proteins of culture filtrate (cf), mycelium (my), and spore (sp) extracts of f. solani were characterized by isoelectrofocusing, sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and ige-specific immunoblotting. cf antigen ... | 1994 | 8199461 |
| three cases of fatal infection due to fusarium solani in patients with cancer. | 1993 | 8286647 | |
| [problems found in the isolation of mycelial fungi (fusarium solani) from blood culture (bacter nr-860)]. | f. solani fungemia is unusual. patients at risk are immunosuppressed, have underlying malignancy or severe debilitating diseases. | 1993 | 8305557 |
| non-ulcerative fungal keratitis diagnosed by posterior lamellar biopsy. | we report a case of fusarium solani keratitis which highlights the difficulties often associated with management of fungal corneal infections. this case demonstrates several unusual and interesting features: the occurrence of deep fungal pathology after superficial injury, the difficulties encountered in attempting to isolate and identify the causative organism, and the protracted course taken by an organism often noted to be rapidly destructive. these features necessitated an individual approac ... | 1993 | 8333933 |
| fungal pathogens in etiology of septic shock in neutropenic patients with cancer (short communication). | during the 3 years from 1989 to 1991, we evaluated the etiology of septic shock cases and infection-associated mortality. a total number of 38 patients was included in the study, according to the criteria for septic shock (ss), (intensive care medicine society, 1989). in 1989, p. aeruginosa and enterobacteriaceae among the pathogens prevailed. in 1990 and 1991, s. aureus, enterococci and fungi were most frequent. from 8 patients with ss in 1990, the shock was due to candida albicans in 1 and to ... | 1993 | 8353326 |
| lung injury by furanoterpenoids isolated from fusarium solani infected sweet potato, ipomea batatas. | intraperitoneal administration (1 mg/kg body/wt./day for 21 days) of crude extract of furanoterpenoids, isolated from f. solani damaged i. batatas caused pulmonary oedema in albino rats. the elevated broncho alveolar lavage (bal) angiotensin converting enzyme (ace), lactate dehydrogenase (ldh) and protein levels indicated lung damage. the estimation of pulmonary extracellular surfactant phospholipids showed an alteration in various phospholipid fractions. | 1993 | 8359843 |
| purification and antipathogenic activity of lipid transfer proteins (ltps) from the leaves of arabidopsis and spinach. | two homogeneous proteins active in vitro against the bacterial pathogen clavibacter michiganensis subsp. sepedonicus were obtained from a crude cell-wall preparation from the leaves of columbia wild-type arabidopsis. the n-terminal amino acid sequences of these proteins allowed their identification as lipid transfer proteins (ltp-a1, ltp-a2); the ltp1-a1 sequence was identical to that deduced from a previously described cdna (embl m80566) and ltp-a2 was quite divergent (44% identical positions). ... | 1993 | 8405465 |
| lipid transfer proteins (nsltps) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. | four homogeneous proteins (cw18, cw20, cw21, cw22) were isolated from etiolated barley leaves by extraction of the insoluble pellet from a tris-hcl (ph 7.5) homogenate with 1.5 m licl and fractionation by reverse-phase high-performance liquid chromatography. all 4 proteins inhibited growth of the pathogen clavibacter michiganensis subsp. sepedonicus (ec50s = 1-3 x 10(-7) m) and had closely related n-terminal amino acid sequences. the complete amino acid sequences of proteins cw18 and cw21 were d ... | 1993 | 8420795 |
| catheter-related fusarium solani fungemia and pulmonary infection in a patient with leukemia in remission. | members of the genus fusarium are ubiquitous fungi uncommonly associated with infection. disseminated fusariosis typically occurs in neutropenic hosts and carries a high mortality rate. we present a case of disseminated fusariosis that is unique in terms of its relative lack of symptomatology despite documentation of fungemia and pulmonary nodules. a summary of the literature is also presented. | 1993 | 8448293 |
| fusarium solani cutaneous infection in a neutropenic patient. | a 47-year-old woman with acute myeloblastic leukemia developed disseminated papules and pustules after chemotherapy. a skin biopsy specimen revealed fungal vasculitis and grew fusarium solani. the patient died despite treatment with amphotericin b. the isolate was resistant to amphotericin b, imidazole and 5-fluocytosine. fusarium should be recognized as a potential cause of deep fungal infection in immunocompromised patients. | 1993 | 8453155 |
| engineering cysteine mutants to obtain crystallographic phases with a cutinase from fusarium solani pisi. | cutinases are extracellular enzymes involved in the disruption of cutine, an insoluble polyester which covers the surface of plants. they belong to a class of serine esterases that are able to hydrolyse fatty acid esters and emulsified triglycerides as efficiently as lipases, but without displaying interfacial activation. classical crystallographic methods for obtaining heavy-atom derivatives failed, so the cutinase structure has been solved exclusively by the multiple isomorphous replacement me ... | 1993 | 8475042 |
| cloning of a novel constitutively expressed pectate lyase gene pelb from fusarium solani f. sp. pisi (nectria haematococca, mating type vi) and characterization of the gene product expressed in pichia pastoris. | since plant-pathogenic fungi must penetrate through pectinaceous layers of the host cell wall, pectin-degrading enzymes are thought to be important for pathogenesis. antibodies prepared against a pectin-inducible pectate lyase (pectate lyase a [pla]) produced by a phytopathogenic fungus, fusarium solani f. sp. pisi (nectria haematococca, mating type vi), was previously found to protect the host from infection. the gene (pela) and its cdna were cloned and sequenced. here we report the isolation o ... | 1995 | 8522511 |
| an exoantigen test for the rapid identification of medically significant fusarium species. | the accurate identification of fusarium species can take 2-3 weeks. preliminary exoantigen studies indicate that a mature culture suspected of being a fusarium species may be immunologically identified 48 h after receipt. exoantigen extracts of 10-day-old slant cultures of fusarium chlamydosporum, fusarium moniliforme (= fusarium verticilloides), fusarium oxysporum, fusarium proliferatum and fusarium solani and partially purified reference homologous and heterologous shake culture extracts (6-we ... | 1995 | 8544080 |
| contribution of cutinase serine 42 side chain to the stabilization of the oxyanion transition state. | cutinase from the fungus fusarium solani pisi is a lipolytic enzyme able to hydrolyze both aggregated and soluble substrates. it therefore provides a powerful tool for probing the mechanisms underlying lipid hydrolysis. lipolytic enzymes have a catalytic machinery similar to those present in serine proteinases. it is characterized by the triad ser, his, and asp (glu) residues, by an oxyanion binding site that stabilizes the transition state via hydrogen bonds with two main chain amide groups, an ... | 1996 | 8555209 |
| recurrence of fusarium solani abscess formation in an otherwise healthy patient. | fusarium spp. are usually considered opportunistic fungi in humans. a case of fusarium solani abscess formation of the foot in an immunocompetent patient in whom recurrence occurred even after intravenous amphotericin b treatment is presented here. | 1995 | 8557391 |
| mixed intranasal infection caused by fusarium solani and a zygomycete in a leukaemic patient. | we report a case of mixed intranasal infection caused by fusarium solani and a zygomycete, with probable sinus and brain involvement. the patient had chronic myelogenous leukaemia and was treated with chemotherapy. diagnosis of the infection was established by direct examination of the intranasal eschar and histopathological study of an excisional biopsy. only f. solani was isolated from both specimens. difficulties in diagnosis and treatment of these infections are discussed. | 1995 | 8559190 |
| identification of elements in the pda1 promoter of nectria haematococca necessary for a high level of transcription in vitro. | expression of the pda1 gene in the ascomycete nectria haematococca mpvi (anamorph: fusarium solani) is induced by exposure of mycelium to pisatin, an isoflavonoid phytoalexin produced by its host plant, garden pea. the pda1 gene encodes a cytochrome p-450 monooxygenase which detoxifies pisatin. regulatory elements controlling transcription from the pda1 promoter were identified using a homologous nectria in vitro transcription system through analysis of 5' deletions, specific oligonucleotide com ... | 1996 | 8569685 |
| tandem mass spectrometry and nuclear magnetic resonance spectroscopy studies of candida bombicola sophorolipids and product formed on hydrolysis by cutinase. | natural mixtures of sophorolipids produced by the yeast candida bombicola have been analyzed by fast atom bombardment (fab)-ms and collision-induced dissociation (cid)-ms. some pure components have been analysed by two-dimensional nmr spectroscopy. the presence of acidic, lactonic, and o-acetylated forms and the position of double bonds in the fatty acid part of these glycolipids can be easily inferred from positive and negative ion fab-mass spectra. details about position of o-acetylation can b ... | 1995 | 8585609 |
| fusarium solani fungal infection of the lateral line canal system in captive scalloped hammerhead sharks (sphyrna lewini) in hawaii. | two of five scalloped hammerhead sharks (sphyrna lewini) captured may 1987 in hawaii (usa) developed granulomatous exudative mycotic dermatitis localized in the lateral line canal system. the lesion initially was noted in the cephalic canals, but over a period of months extended into the lateral canal. fusarium solani and vibrio spp. were isolated from the canal exudate of both sharks. bacterial colonies were not observed in the canal walls or surrounding tissues. fusarium solani infection resul ... | 1995 | 8592393 |
| fusarium polycaprolactone depolymerase is cutinase. | polycaprolactone (pcl), a synthetic polyester, is degraded by a variety of microorganisms, including some phytopathogens. many phytopathogens secrete cutinase, a serine hydrolase that degrades cutin, the structural polymer of the plant cuticle. we compared wild-type strains and a cutinase-negative gene replacement mutant strain of fusarium solani f. sp. pisi (d. j. stahl and w. schäfer, plant cell 4:621-629, 1992) and a wild-type strain of fusarium moniliforme to show that fusarium cutinase is a ... | 1996 | 8593048 |
| studies on shared antigenic/allergenic components among fungi. | fungal allergens have been found to be one of the most prevalent aeroallergens in india. knowledge of shared/unique components among different fungi is necessary for proper diagnosis and treatment of patients allergic to fungi. in the present study, crude extracts (ce) of 11 common fungi (alternaria alternata, aspergillus flavus, asp. fumigatus, asp. niger, asp. tamarii, asp. versicolor, cladosporium herbarum, curvularia lunata, mucor hiemalis, penicillium citrinum, and fusarium solani) were cha ... | 1995 | 8607563 |
| fusarium solani dnase is a signal for increasing expression of nonhost disease resistance response genes, hypersensitivity, and pisatin production. | the inoculation of pea endocarp tissue with the bean pathogen fusarium solani f. sp. phaseoli results in a non-host resistance response causing a complete cessation of fungal growth within 6 to 8 h. in addition to previously reported elicitation by chitosan, we now report that components of this response are also induced by a dnase released from this fungus. a single band of protein corresponding with dnase activity elicits phytoalexin production and the accumulation of rna homologous with the p ... | 1995 | 8664496 |
| oxidative stress in rat liver and lung induced by furanoterpenoids isolated from fusarium solani infected sweet potatoes. | a crude extract containing some toxic furanoterpenoids was isolated from f. solani infected sweet potatoes. chronic administration of the crude extract to male albino rats at a dosage of 1 mg/kg body weight/day for 21 days brought about a sharp increase in the thiobarbituric acid reactive substances and a depression of glutathione levels in the lung and liver homogenates. the antioxidant defense system was affected as evident from a significant fall in the activities of the enzymes, superoxide d ... | 1996 | 8698409 |
| cloning and characterization of mycovirus double-stranded rna from the plant pathogenic fungus, fusarium solani f. sp. robiniae. | a mycovirus (named fusov) from the phytopathogenic fungus, fusarium solani f. sp. robiniae suf704, has two kinds of double-stranded (ds) rna genomes, designated m1 and m2. the cdnas were constructed from fusov genomic dsrnas. the sequences of m1 and m2 cdnas comprised 1645 and 1445bp, respectively. sequence analysis showed that each dsrna had a single long open reading frame (orf) on only one of the strands. m1 orf encodes a 519-amino acid residue polypeptide with a predicted molecular mass of 6 ... | 1996 | 8704307 |
| proteolytic processing of class iv chitinase in the compatible interaction of bean roots with fusarium solani. | three chitinase isoenzymes, pvchie, pvchif, and pvchig (molecular masses 29, 28, 27 kd, respectively), were purified from bean (phaseolus vulgaris l. cv saxa) roots infected with the fungal pathogen fusarium solani f. sp. phaseoli, and their amino acid sequence was partially determined. all sequences from all three isoenzymes exactly matched deduced amino acid sequences of the bean class iv chitinase pvchi4, formerly called pr4. the n terminus of pvchif mapped to the hinge region, and the n term ... | 1996 | 8756497 |
| susceptibility of clinical isolates of fusarium to antifungal drugs. | the inhibitory activities of amphotericin b, fluconazole, itraconazole, miconazole, ketoconazole and terbinafine against nine isolates from clinically apparent infections of fusarium solani, four isolates of fusarium moniliforme and 10 isolates of fusarium oxysporum were determined with an agar diffusion method (neosensitabs) and an agar dilution method. the inhibition zones obtained with antifungal neosensitabs need very careful interpretation. we did not find a good correlation between the aga ... | 1996 | 8786755 |
| amphotericin b lipid complex treatment of a leukemic child with disseminated fusarium solani infection. | we report a case of fusarium solani infection in a 2 1/2-year-old girl with acute lymphoblastic leukemia and severe neutropenia. the infection, initially limited to her sinuses, became disseminated, as evidenced by the development of a characteristic cutaneous lesion on her left leg after a cumulative dose of amphotericin b of 18.3 mg/kg. amphotericin b lipid complex (ablc), 5 mg/kg/day, in conjunction with repeated surgical debridement of the sinuses and correction of neutropenia with granulocy ... | 1996 | 8804544 |
| identification of a novel peld gene expressed uniquely in planta by fusarium solani f. sp. pisi (nectria haematococca, mating type vi) and characterization of its protein product as an endo-pectate lyase. | antibodies prepared against a pectin-inducible pectate lyase (pla) produced by a phytopathogenic fungus fusarium solani f. sp. pisi (nectria haematococca, mating type vi) were previously found to protect the host from infection. the gene (pela) and two of its homologs were cloned and sequenced. here we report the isolation of a new pectate lyase gene, peld, from a genomic library of f. solani pisi. a 1.5-kb dna fragment containing peld and its flanking regions was sequenced. the nucleotide seque ... | 1996 | 8806739 |
| analysis of determinants of binding and transcriptional activation of the pisatin-responsive dna binding factor of nectria haematococca. | pisatin is a fungistatic isoflavonoid produced by garden pea. field isolates of the ascomycete nectria haematococca mating population vi (anamorph: fusarium solani) that are highly virulent on pea have been found to possess the pda1 gene encoding a pisatin detoxifying activity. expression of pda1 is specifically and highly induced by exposure of mycelia to pisatin. a pisatin-responsive dna-binding activity has previously been identified with properties suggestive of a transcriptional regulator o ... | 1996 | 8850087 |
| immobilization of a recombinant cutinase by entrapment and by covalent binding. kinetic and stability studies. | fusarium solani pisi recombinant cutinase, immobilized by entrapment in calcium alginate and by covalent binding on porous silica, was used to catalyze the hydrolysis of tricaprylin. the influence of relevant parameters on the catalytic activity such as ph, temperature, and the substrate concentration were studied. cutinase immobilized by entrapment presented a michaelis-menten kinetics for tricaprylin concentrations up to 200 mm. at higher concentrations of substrate, inhibition was observed. f ... | 1996 | 8933716 |
| kinetic studies of fusarium solani cutinase in a gas/solid system. | 1996 | 8958113 | |
| the effect of pre- and pro-sequences and multicopy integration on heterologous expression of the fusarium solani pisi cutinase gene in aspergillus awamori. | a synthetic derivative of the cutinase cdna of fusarium solani pisi was expressed in aspergillus awamori using the a. awamori endoxylanase ii (exla) promoter and terminator. the influence of the origin of the pre-sequence and the presence of a pro-sequence on the efficiency of extracellular cutinase production was analysed in single-copy transformants containing an expression cassette integrated at the pyrg locus. transformants containing a construct encoding a direct, inframe fusion of the xyla ... | 1996 | 8987467 |
| dynamics of fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants. | in characterizing mutants and covalently inhibited complexes of fusarium solani cutinase, which is a 197-residue lipolytic enzyme, 34 variant structures, crystallizing in 8 different crystal forms, have been determined, mostly at high resolution. taking advantage of this considerable body of information, a structural comparative analysis was carried out to investigate the dynamics of cutinase. surface loops were identified as the major flexible protein regions, particularly those forming the act ... | 1996 | 8990497 |
| effect of gamma radiation on the survival of fungal and actinomycetal florae contaminating medicinal plants. | this study evaluates the effect of gamma radiation on the viability of fungi and actinomycetes that contaminate medicinal plants. the relationship between the total lipids of some fungi and actinomycetes and their sensitivity to gamma radiation is also investigated. the date reveal that the viable counts of these florae decrease approximately exponentially with the radiation dose, the effective dose for the elimination of these microorganisms being about 5 kgy for all the medicinal plants under ... | 1997 | 9022214 |
| crystal structure of cutinase covalently inhibited by a triglyceride analogue. | cutinase from fusarium solani is a lipolytic enzyme that hydrolyses triglycerides efficiently. all the inhibited forms of lipolytic enzymes described so far are based on the use of small organophosphate and organophosphonate inhibitors, which bear little resemblance to a natural triglyceride substrate. in this article we describe the crystal structure of cutinase covalently inhibited by (r)-1,2-dibutyl-carbamoylglycero-3-o-p-nitrophenylbutyl-phos phonate, a triglyceride analogue mimicking the fi ... | 1997 | 9041628 |
| in-vitro antifungal susceptibility of clinical and environmental fusarium spp. strains. | the mics of amphotericin b, miconazole, ketoconazole, flucytosine, itraconazole and fluconazole for 19 isolates of fusarium oxysporum, 16 fusarium solani, seven fusarium verticilliodes, four fusarium proliferatum, four fusarium dimerum, three fusarium equiseti, and one each of the following species: fusarium graminearum, fusarium chlamydosporum, fusarium semitectum, fusarium avenaceum and fusarium subglutinans were determined by a broth microdilution method. thirty-eight of these isolates were o ... | 1997 | 9069536 |
| fusarium solani fungemia in a bone marrow transplant recipient. | disseminated fusarium solani infection, with its distinctive skin lesions, is an emerging cause of mortality in bone marrow transplant recipients worldwide. however, it has never been reported before in taiwan. we report a 21-year-old man with disseminated fusariosis who developed fever, myalgia and generalized erythematous papules on day 8 after undergoing allogeneic bone marrow transplantation for severe aplastic anemia. histopathology of the skin lesion revealed mycotic emboli. cultures of bo ... | 1997 | 9071840 |
| effect of disruption of a cutinase gene (cuta) on virulence and tissue specificity of fusarium solani f. sp. cucurbitae race 2 toward cucurbita maxima and c. moschata. | a 3.9-kb genomic dna fragment from the cucurbit pathogen fusarium solani f. sp. cucurbitae race 2 was cloned. sequence analysis revealed an open reading frame of 690 nucleotides interrupted by a single 51-bp intron. the nucleotide and predicted amino acid sequences showed 92 and 98% identity, respectively, to those of the cuta gene of the pea pathogen f. solani f. sp. pisi. a gene replacement vector was constructed and used to generate cuta- mutants that were detected with a polymerase chain rea ... | 1997 | 9100380 |
| disseminated fusariosis. | a 7-year-old boy with t cell acute lymphoblastic leukemia developed disseminated hyalohyphomycosis due to fusarium solani. the clinical features included fever, severe myalgia, documented fungemia with f. solani, an ecthyma gangrenosum-like lesion next to a peripheral venous catheter, and disseminated pustules. severe neutropenia due to chemotherapy was the most relevant risk factor. histopathologic study of the ecthyma gangrenosum-like lesion, as well as pustular lesions, revealed epidermal nec ... | 1996 | 9122067 |
| cutaneous presentation of fusarium solani infection in a bone marrow transplant recipient. | 1996 | 9135599 | |
| cloning of cutinase transcription factor 1, a transactivating protein containing cys6zn2 binuclear cluster dna-binding motif. | hydroxy fatty acids from plant cutin were shown previously to induce the expression of the cutinase gene via a palindromic sequence located at -159 base pairs of the cutinase gene in fusarium solani f. sp. pisi (nectria hematococca mating type vi). of the two overlapping palindromes in this sequence, palindrome 2 was found to be essential for the inducibility of cutinase by hydroxy fatty acids. screening of a phage expression library with the concatenated palindrome 2 as probe detected a distinc ... | 1997 | 9139694 |
| invasive fusarium infections: a retrospective survey of 31 cases. the french 'groupe d'etudes des mycoses opportunistes' gemo. | a retrospective study was conducted in france to investigate fusarium infections which are now recognized as emerging opportunistic infections. the clinical and mycological findings for 31 cases diagnosed between 1984 and 1993 by members of the french groupe d'etudes des mycoses opportunistes were analysed. all suffered from haematological disease, most often acute leucaemia (n = 19). twenty-two had received cytostatic chemotherapy and ten had undergone bone marrow transplantation. prolonged apl ... | 1997 | 9147270 |
| atomic resolution (1.0 a) crystal structure of fusarium solani cutinase: stereochemical analysis. | x-ray data have been recorded to 1.0 a resolution from a crystal of fusarium solani cutinase using synchrotron radiation and an imaging-plate scanner. the anisotropic treatment of thermal motion led to a fivefold increase in accuracy and to a considerable quality improvement in the electron density maps with respect to an intermediate isotropic model. the final model has an r-factor of 9.4%, with a mean coordinate error of 0.021 a, as estimated from inversion of the least-squares matrix. the ava ... | 1997 | 9175860 |
| polyhexamethylene biguanide (phmb) in the treatment of experimental fusarium keratomycosis. | we wanted to determine whether topical polyhexamethylene biguanide (phmb) 0.02% was effective in the treatment of experimental fusarium keratomycosis in rabbits. | 1997 | 9220243 |
| fungaemia due to fusarium spp. in cancer patients. | five cases of fungaemia due to fusarium spp. in cancer patients are described. two were breakthrough cases, despite ongoing therapy with amphotericin b. three were caused by fusarium solani, one by f. oxysporum and one by f. dimerum. four patients died, three of them despite therapy with amphotericin b for between 5-37 days. we describe only the second reported case of f. dimerum fungaemia. since 1972, 93 cases of systemic infection with fusarium spp. have been described: 43 had positive blood c ... | 1997 | 9253703 |
| in vitro antifungal activity of pneumocandin l-743,872 against a variety of clinically important molds. | the in vitro activity of the new antifungal drug pneumocandin l-743,872 against 55 isolates of clinically important molds was examined by an adapted macrobroth dilution method for yeasts. pneumocandin l-743,872 exhibited in vitro antifungal activity against alternaria sp., aspergillus flavus, aspergillus fumigatus, curvularia lunata, exophiala jeanselmei, fonsecaea pedrosoi, paecilomyces variotii, and scedosporium apiospermum. the drug appeared to lack significant in vitro inhibitory activity ag ... | 1997 | 9257774 |
| immunohistologic identification of aspergillus spp. and other hyaline fungi by using polyclonal fluorescent antibodies. | isolation and identification of pathogenic aspergillus and fusarium spp. from clinical materials provide the most accurate means for establishing a diagnosis of infections by these molds. such efforts, however, are not always successful. histologic diagnosis also has its limitations. in vivo the hyphae of aspergillus and fusarium spp. are very similar and their in situ manifestations are not pathognomonic. to improve the histologic diagnosis of infections by aspergillus and fusarium species, we ... | 1997 | 9276388 |
| [resolution of a disseminated infection by fusarium solani in a patient with acute promyelocytic leukemia and granulocytopenia secondary to chemotherapy by the combined use of g-csf and liposomal amphotericin b]. | 1997 | 9289532 | |
| cloning and expression of cdna encoding a mitogen-activated protein kinase from a phytopathogenic filamentous fungus. | we have cloned a mitogen-activated protein kinase (mapk) designated fusarium solani f. sp. pisi mitogen-activated protein kinase (fsmapk) from the phytopathogenic filamentous fungus f. solani f. sp. pisi t8 strain. a single open reading frame (orf) of 1068 bp encoding a polypeptide of 355 amino acids (aa) with a predicted molecular mass of 41,194 da was found in the cloned 1583-bp cdna insert. fsmapk is highly homologous to spk1 of fission yeast, fus3 of budding yeast, mserk1 of alfalfa, sur-1 o ... | 1997 | 9305760 |
| scanning slit confocal microscopy of fungal keratitis. | in vivo scanning slit confocal microscopy was performed in a patient with fusarium solani keratitis. morphologically distinctive abundant filamentous structures were observed intrastromally. confocal microscopy of the culture plate growing f solani from the patient's corneal scraping revealed filaments morphologically similar to the filaments observed in vivo. after 1 week of medical therapy, subsequent confocal microscopy showed an increased load of filaments, supporting the decision to perform ... | 1997 | 9366681 |
| primary pulmonary involvement of fusarium solani in a lung transplant recipient. | a case of cavitary lung disease caused by fusarium solani in a lung transplant recipient is presented. a mechanism for development of this infection is proposed. lipid complex amphotericin b (abelcet) was effective in eradicating this infection. to our knowledge, invasive lung disease caused by the fusarium species has not been previously reported in a solid organ transplant recipient. | 1997 | 9377931 |
| 1h, 13c, and 15n resonance assignments of fusarium solani pisi cutinase and preliminary features of the structure in solution. | essentially complete (96%) sequence-specific assignments were made for the backbone and side-chain 1h, 13c, and 15n resonances of fusarium solani pisi cutinase, produced as a 214-residue heterologous protein in escherichia coli, using heteronuclear nmr techniques. three structural features were noticed during the assignment. (1) the secondary structure in solution corresponds mostly with the structure from x-ray diffraction, suggesting that both structures are globally similar. (2) the hn of ala ... | 1997 | 9385640 |
| pectate lyase peli of erwinia chrysanthemi 3937 belongs to a new family. | erwinia chrysanthemi 3937 secretes five major isoenzymes of pectate lyases encoded by the pel4, pelb, pelc, peld, and pele genes and a set of secondary pectate lyases, two of which, pell and pelz, have been already identified. we cloned the peli gene, encoding a ninth pectate lyase of e. chrysanthemi 3937. the peli reading frame is 1,035 bases long, corresponding to a protein of 344 amino acids including a typical amino-terminal signal sequence of 19 amino acids. the purified mature peli protein ... | 1997 | 9393696 |
| cutaneous hyalohyphomycosis caused by fusarium solani in a loggerhead sea turtle (caretta caretta l.). | fusarium solani was reported as the agent of a cutaneous infection in an injured sea turtle collected in the mediterranean sea. the turtle was treated with both a topical 10% solution of iodine in alcohol and ketoconazole. the source of the causal agent was traced to the sand in the tank in which the turtle was maintained. the strain was only sensitive in vitro to amphotericin b and was resistant to 5-fluorocytosine, fluconazole, itraconazole, and ketoconazole. | 1997 | 9399554 |
| treatment of fungal corneal ulcers with amphotericin b ointment. | to report two patients with severe fungal corneal ulcers who were treated successfully with topical amphotericin b ointment. | 1997 | 9402830 |
| in vitro activity of the new triazole voriconazole (uk-109,496) against opportunistic filamentous and dimorphic fungi and common and emerging yeast pathogens. | the in vitro antifungal activity of a new triazole derivative, voriconazole, was compared with those of itraconazole and amphotericin b against 67 isolates of aspergillus flavus, aspergillus fumigatus, bipolaris spp., fusarium oxysporum, fusarium solani, pseudallescheria boydii, rhizopus arrhizus, blastomyces dermatitidis, histoplasma capsulatum, and sporothrix schenckii. the in vitro activities of voriconazole were also compared with those of amphotericin b, fluconazole, and itraconazole agains ... | 1998 | 9431946 |
| molecular cloning of tvdao1, a gene encoding a d-amino acid oxidase from trigonopsis variabilis and its expression in saccharomyces cerevisiae and kluyveromyces lactis. | the dao1 gene of trigonopsis variabilis encoding a d-amino acid oxidase (ec 1.4.3.3) was isolated from genomic clones selected for their specific hybridization to synthetic oligodeoxyribonucleotide probes based on regions of the enzyme that have been conserved through evolution. the nucleotide sequence of the gene predicts a protein with similarities to human, pig, rabbit, mouse and fusarium solani d-amino acid oxidases. the open reading frame of the t. variabilis dao1 gene was interrupted by an ... | 1997 | 9434346 |
| a unique case of cryptococcus laurentii keratitis spread by a rigid gas permeable contact lens in a patient with onychomycosis. | we report the first case of fungal keratitis caused by cryptococcus laurentii, a nonneoformans species. | 1998 | 9436889 |
| exploring hydrophobic sites in proteins with xenon or krypton. | x-ray diffraction is used to study the binding of xenon and krypton to a variety of crystallised proteins: porcine pancreatic elastase; subtilisin carlsberg from bacillus licheniformis; cutinase from fusarium solani; collagenase from hypoderma lineatum; hen egg lysozyme, the lipoamide dehydrogenase domain from the outer membrane protein p64k from neisseria meningitidis; urate-oxidase from aspergillus flavus, mosquitocidal delta-endotoxin cytb from bacillus thuringiensis and the ligand-binding do ... | 1998 | 9443341 |
| fusarium solani infection in a patient with acute myelogenous leukemia--a case report. | multiple necrotizing skin lesions due to fusarium solani in an elderly man with acute myelogenous leukemia is described. | 1997 | 9444872 |
| allergic fungal sinusitis in the southeastern usa: involvement of a new agent epicoccum nigrum ehrenb. ex schlecht. 1824. | alternaria alternata, aspergillus spp., bipolaris spicifera, curvularia lunata, epicoccum nigrum and fusarium solani were isolated repeatedly from groups of patients among 96 diagnosed with allergic fungal sinusitis (afs). epicoccum nigrum was obtained consistently from four patients, one of whom yielded mycelial masses consistent in morphology with e. nigrum. fifteen of the predominant fungi recovered from air samples from selected patients' residences included the same species isolated from th ... | 1997 | 9467107 |
| two-dimensional nmr experiments for the assignment of aromatic side chains in 13c-labeled proteins | as aromatic residues very often are part of the hydrophobic core of proteins, the unambiguous assignment of the aromatic proton resonances is essential for an accurate and precise structure determination. instead of transferring 1hbeta coherence to the aromatic protons via 13cgamma like in a number of published methods, in our new experiments the 13cgamma resonances are first correlated with the 1hbeta chemical shifts in one experiment and then with the aromatic proton resonances in four other e ... | 1998 | 9469899 |
| delayed-onset fungal keratitis after endophthalmitis. | this study aimed to present two patients with delayed-onset, endogenous fungal keratitis after treatment for fungal endophthalmitis after cataract surgery. | 1998 | 9479284 |
| structural characterization of neutral glycosphingolipids from fusarium species. | glycosphingolipids were extracted from hyphae of fusarium solani and from an unnamed fusarium species, and were purified by silica and iatrobead column chromatography. their structures were determined by compositional analysis, nuclear magnetic resonance spectroscopy, gas chromatography/mass spectrometry and by fast atom bombardment mass spectrometry of the native and peracetylated materials, which defined their sugar, long-chain base and fatty acid compositions. the locations of the double bond ... | 1998 | 9507119 |
| detection of fungemia from blood cultures using the bactec 9240 instrument. | from january 1995 to november 1996, 14,623 resin-containing blood culture vials were tested with the bactec fluorescent series instrument. a total of 1560 microorganisms were recovered. 48 of the microorganisms were fungi. we could demonstrate the ability of the bactec 9240 to detect cases of fungemia with candida species and moulds such as aspergillus fumigatus (4 cases) and fusarium solani (1 case). | 1998 | 9532264 |
| polymerase chain reaction and restriction fragment length polymorphism mediated detection and speciation of candida spp causing intraocular infection. | to determine the usefulness of polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) analysis in the identification and speciation of candida spp that causes ocular infection. | 1998 | 9579465 |
| packing forces in nine crystal forms of cutinase. | during the characterization of mutants and covalently inhibited complexes of fusarium solani cutinase, nine different crystal forms have been obtained so far. protein mutants with a different surface charge distribution form new intermolecular salt bridges or long-range electrostatic interactions that are accompanied by a change in the crystal packing. the whole protein surface is involved in the packing contacts and the hydrophobicities of the protein surfaces in mutual contact turned out to be ... | 1998 | 9593202 |
| diagnosis of fusarium keratitis in an animal model using the polymerase chain reaction. | the purpose of this study was apply the polymerase chain reaction (pcr) to develop a sensitive, specific, and rapid test to diagnose fusarium keratitis. fusarium is the most common cause of fungal corneal infection in some parts of the world. it is often difficult to establish that a keratitis is due to fungal infection. | 1998 | 9602631 |
| disseminated fusarium solani infection with endocarditis in a lung transplant recipient. | eleven days after double lung transplantation for cystic fibrosis, an 18-year-old patient developed a disseminated fusarium solani infection with tricuspid valve endocarditis. this infection occurred under fluconazole and immunosuppressive therapy with cyclosporin, prednisone and azathioprine, with a normal leucocyte count. liposomal amphotericin b allowed blood culture negativation. the patient died from a bacterial septic shock. | 1998 | 9610136 |
| keratin degradation by scytalidium species and fusarium solani. | the keratin degradation capability of some non-dermatophytic filamentous fungi, viz scytalidium dimidiatum types a and b, s. hyalinum, s. japonicum and fusarium solani, was investigated using nail clippings and keratin powder as substrates. representative isolates of the dermatophytes trichophyton rubrum and t. mentagrophytes were also tested. the two morphological types of s. dimidiatum were almost equally effective in the degradation of substrates, 42-43% nail clippings and 24-26% keratin. scy ... | 1998 | 9610139 |
| rapid extraction of genomic dna from medically important yeasts and filamentous fungi by high-speed cell disruption. | current methods of dna extraction from different fungal pathogens are often time-consuming and require the use of toxic chemicals. dna isolation from some fungal organisms is difficult due to cell walls or capsules that are not readily susceptible to lysis. we therefore investigated a new and rapid dna isolation method using high-speed cell disruption (hscd) incorporating chaotropic reagents and lysing matrices in comparison to standard phenol-chloroform (pc) extraction protocols for isolation o ... | 1998 | 9620390 |
| bacterial-fungal biofilms in flowing water photo-processing tanks. | a complex seven species model community, including bacteria and fungi, was selected from organisms isolated from the walls of an industrial flowing water system. growth rates of the species were determined in single and mixed batch culture growth. the rates were found to be significantly higher in mixed culture for pseudomonas alcaligenes and flavobacterium indologenes and higher in single culture for xanthomonas maltophilia, rhodotorula glutinis and fusarium solani, whereas no significant diffe ... | 1998 | 9633659 |
| expression and secretion of defined cutinase variants by aspergillus awamori. | several cutinase variants derived by molecular modelling and site-directed mutagenesis of a cutinase gene from fusarium solani pisi are poorly secreted by saccharomyces cerevisiae. the majority of these variants are successfully produced by the filamentous fungus aspergillus awamori. however, the l51s and t179y mutations caused reductions in the levels of extracellular production of two cutinase variants by a. awamori. metabolic labelling studies were performed to analyze the bottleneck in enzym ... | 1998 | 9687432 |
| comparison of in vitro activities of the new triazole sch56592 and the echinocandins mk-0991 (l-743,872) and ly303366 against opportunistic filamentous and dimorphic fungi and yeasts. | the in vitro antifungal activities of sch56592, mk-0991, and ly303366 against 83 isolates of acremonium strictum, aspergillus flavus, aspergillus fumigatus, aspergillus terreus, bipolaris spp., blastomyces dermatitidis, cladophialophora bantiana, fusarium oxysporum, fusarium solani, histoplasma capsulatum, phialophora spp., pseudallescheria boydii, rhizopus arrhizus, scedosporium prolificans, and sporothrix schenckii were compared. the in vitro activities of these agents against 104 isolates of ... | 1998 | 9738049 |
| structure-in vitro activity relationships of pentamidine analogues and dication-substituted bis-benzimidazoles as new antifungal agents. | twenty analogues of pentamidine, 7 primary metabolites of pentamidine, and 30 dicationic substituted bis-benzimidazoles were screened for their inhibitory and fungicidal activities against candida albicans and cryptococcus neoformans. a majority of the compounds had mics at which 80% of the strains were inhibited (mic80s) comparable to those of amphotericin b and fluconazole. unlike fluconazole, many of these compounds were found to have potent fungicidal activity. the most potent compound again ... | 1998 | 9756747 |
| in vitro antifungal activities of a series of dication-substituted carbazoles, furans, and benzimidazoles. | aromatic dicationic compounds possess antimicrobial activity against a wide range of eucaryotic pathogens, and in the present study an examination of the structures-functions of a series of compounds against fungi was performed. sixty-seven dicationic molecules were screened for their inhibitory and fungicidal activities against candida albicans and cryptococcus neoformans. the mics of a large number of compounds were comparable to those of the standard antifungal drugs amphotericin b and flucon ... | 1998 | 9756748 |
| identification of functional and unfolding motions of cutinase as obtained from molecular dynamics computer simulations. | the implementation of cutinase from fusarium solani pisi as a fat-stain removing ingredient in laundry washing is hampered by its unfolding in the presence of anionic surfactants. in this work we present molecular dynamics (md) computer simulations on cutinase and analysis procedures to distinguish the movements related to its functional behavior (e.g., substrate binding) from those related to the unfolding of the enzyme. two kinds of md-simulations were performed: a simulation mimicking the the ... | 1998 | 9779792 |
| [fusarium solani infection in a hematology unit. study of 2 cases and 2 isolations]. | 1998 | 9810555 | |
| cutinase binding and activity at the triolein-water interface monitored by oil drop tensiometry. | changes of the oil-water interfacial tension resulting from binding of fusarium solani pisi cutinase and subsequent lipid hydrolysis were investigated using the oil drop technique. an elisa was developed to determine the amount of cutinase bound to the triolein-water interface after biotinylation of the enzyme. cutinase irreversibly adsorbs to a maximum value of about 2 mg/m2. a minimal specific activity of 110 mumol/min/mg was calculated for cutinase acting on a single oil droplet, which is clo ... | 1998 | 9853365 |
| protein engineering the surface of enzymes. | the protein surface is the interface through which a protein senses the external world. its composition of charged, polar and hydrophobic residues is crucial for the stability and activity of the protein. the charge state of seven of the twenty naturally occurring amino acids is ph dependent. a total of 95% of all titratable residues are located on the surface of soluble proteins. in evolutionary related families of proteins such residues are particularly prone to substitutions, insertions and d ... | 1998 | 9866858 |
| antisporulating and respiration-inhibitory effects of essential oils on filamentous fungi. | sporulation of four species of filamentous fungi, namely aspergillus fumigatus, fusarium solani, penicillium expansum and rhizopus oryzae, was suppressed by gaseous contact with citron, lavender and thyme oils and, to a lesser extent, by that of perilla and tea tree oils. lemongrass and cinnamon bark oils were scarcely active. the antisporulating effect of the essential oils was not observed when they were applied as a solution, indicating that their vapours were the active form. moreover, expos ... | 1998 | 9916464 |
| soil fungi of some low-altitude desert cotton fields and ability of their extracts to inhibit aspergillus flavus. | soil is presumed to be a major source of inoculum for aspergillus flavus which contaminates cottonseed and produces the potent carcinogen, aflatoxin. little is known about the mycoflora of the low desert soils of cotton fields where aflatoxin is a chronic problem. in this study, soils from cotton fields in southwestern arizona and south-eastern california were assayed for filamentous fungi. forty-two taxa, predominantly in the genera aspergillus, penicillium and fusarium, were isolated. to deter ... | 1998 | 9926421 |
| cloning and characterization of pl1 encoding an in planta-secreted pectate lyase of fusarium oxysporum. | a pectate lyase (pl1) from the tomato vascular wilt pathogen fusarium oxysporum f.sp. lycopersici was previously characterized, and evidence was obtained for its production in planta. the gene encoding pl1 was isolated from a genomic library of f. oxysporum f. sp. lycopersici. pl1 encodes a 240 amino-acid polypeptide with one putative n-glycosylation site and a 15 amino-acid n-terminal signal peptide. pl1 showed 89%, 67%, 55% and 56% identity with the products of the fusarium solani f.sp. pisi p ... | 1999 | 10022947 |
| treatment of murine fusariosis with sch 56592. | doses of 10 to 100 mg of the azole antifungal agent sch 5692/kg of body weight/day were studied in immunocompetent mice as therapy for systemic infection by fusarium solani. treatment was begun 1 h after intravenous infection and continued daily for 4 or 13 doses. prolongation of survival and organ clearance were dependent on both the dose and the duration of sch 56592 therapy, with the best results seen at 50 and 100 mg/kg/day. the results at the highest doses of sch 56592 used (50 or 100 mg/kg ... | 1999 | 10049271 |
| in-vitro activity of voriconazole, itraconazole and amphotericin b against filamentous fungi. | the in-vitro fungistatic and fungicidal activities of voriconazole were compared with those of itraconazole and amphotericin b. mics for 110 isolates belonging to 11 species of filamentous fungi were determined by a broth microdilution adaptation of the method recommended by the national committee for clinical laboratory standards. minimum lethal concentrations (mlcs) of the three antifungal agents were also determined. the mic ranges of the three compounds were comparable for aspergillus flavus ... | 1998 | 10052897 |
| comparison of interferon-gamma, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor for priming leukocyte-mediated hyphal damage of opportunistic fungal pathogens. | proinflammatory cytokines have been proposed as adjunctive therapeutic agents to enhance the host immune response during infections caused by opportunistic fungi. the study compared the differential in vitro priming effects of interferon-gamma (ifn-gamma), granulocyte colony-stimulating factor (g-csf), and granulocyte-macrophage colony-stimulating factor (gm-csf) on hyphal damage of opportunistic fungi mediated by isolated neutrophils (polymorphonuclear leukocytes, pmnl) and buffy coat cells (po ... | 1999 | 10068606 |
| comparative antimicrobial efficacy of multi-purpose lens care solutions using the fda's revised guidance document for industry: stand-alone primary criteria. | we evaluated six single-bottle, multi-purpose lens care solutions and a two component lens care system for disinfection efficacy according to the stand-alone primary criteria within the recently published u.s. food and drug administration (fda) guidelines. | 1999 | 10073638 |
| endogenous endophthalmitis by fusarium solani: an animal experimental model. | an experimental model of endophthalmitis by fusarium solani in immunocompetent mice that could be useful for evaluating the efficacy of different treatments and the pathogenicity of the fungus in ocular structures was established. five clinical isolates of f. solani were injected into the lateral tail vein of groups of 20 mice, in order to produce systemic infection with ocular infection. inocula of 5 x 10(6) conidia per mouse were used. the eyes of the animals that died were enucleated for hist ... | 1998 | 10075492 |
| modulation of neutrophil-mediated activity against the pseudohyphal form of candida albicans by granulocyte colony-stimulating factor (g-csf) administered in vivo. | renewed interest in neutrophil transfusions has emerged with the development and clinical use of granulocyte colony-stimulating factor (g-csf). g-csf not only increases neutrophil (polymorphonuclear leukocyte, pmnl) production but also modulates various physiological properties of pmnl. the effects of g-csf on pmnl-mediated fungicidal activity were evaluated by administration of g-csf (300 micrograms/day subcutaneously) to 5 healthy volunteers for 6 days. g-csf significantly enhanced pmnl-mediat ... | 1999 | 10191242 |
| interfacial binding of cutinase rather than its catalytic activity determines the steady state interfacial tension during oil drop lipid hydrolysis. | hydrolysis of triglycerides by cutinase from fusarium solani pisi causes in oil drop tensiometer experiments a decrease of the interfacial tension. a series of cutinase variants with amino acid substitutions at its molecular surface yielded different values of the steady state interfacial tension. this tension value poorly correlated with the specific activity as such nor with the total activity (defined as the specific activity multiplied by the amount of enzyme bound) of the cutinase variants. ... | 1999 | 10192932 |