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[bcmf4 gene, encoding a leucine-rich repeat protein, plays a role in male fertility in chinese cabbage-pak-choi].the bcmf4 (brassica campestris male fertility 4) gene was previously isolated from the fertile b line of chinese cabbage-pak-choi (brassica campestris ssp. chinensis var. communis, syn. b. rapa ssp. chinensis var. communis). in the present paper, based on the cdna sequence of bcmf4, primers were designed and used to amplify two fragments from the cdna of flower buds of chinese cabbage-pak-choi. two produced fragments were introduced separately into binary vector pbi121 in antisense and sense ori ...200617098713
molecular and functional characterization of a cyclophilin with antifungal activity from chinese cabbage.an antifungal protein that inhibits the growth of filamentous fungal pathogens was isolated from chinese cabbage (brassica campestris l. ssp. pekinensis) by affinity chromatography on affi-gel blue gel and ion exchange chromatography on cm-sepharose. the n-terminal amino acid sequence of the protein was highly homologous to that of plant cyclophilins and consequently the protein was denoted as c-cyp. to understand the antifungal activity of c-cyp, we isolated a cdna encoding its gene from a chin ...200717194440
bcmf11, a putative pollen-specific non-coding rna from brassica campestris ssp. chinensis.a full-length cdna, bcmf11, has been cloned from chinese cabbage (brassica campestris l. ssp. chinensis makino) using rapid amplification of the cdna ends (race) based on a pollen-specific cdna fragment (dn237921). the bcmf11 cdna has a total length of 828bp with poly (a) tail. analysis of the sequence demonstrated that bcmf11 is a novel non-coding rna which has no prominent open reading frame (orf) or coding capacity. no significant similarities were observed between bcmf11 and previously publi ...200717207554
comparative studies on s-glycoproteins purified from different s-genotypes in self-incompatible brassica species ii. immunological specificities.antisera were prepared by immunization of apparently purified s-glycoproteins; one from an s allele of brassica campestris and two from s alleles of b. oleracea. each antiserum was reactive not only with the homologous s-glycoprotein but also with the heterologous ones, i.e. with the s-glycoproteins of the other s alleles of the same locus. in double diffusion tests, a spur against the heterologous s-glycoproteins suggested heterogeneity of the glycoproteins. the heterogeneity appears to involve ...198217246075
design, synthesis, and quantitative structure-activity relationship study of herbicidal analogues of pyrazolo[5,1-d][1,2,3,5]tetrazin-4(3h)ones.a series of pyrazolo[5,1-d][1,2,3,5]tetrazin-4(3h)one derivatives were designed, synthesized, and evaluated for their herbicidal activities where some of these compounds provided >80% control of brassica campestris at 10 microg/ml. quantitative structure-activity relationship studies were performed on these compounds using physicochemical parameters (electronic, verloop, or hydrophobic) as independent parameters and herbicidal activity as a dependent parameter, where herbicidal activity correlat ...200717300154
pedobacter suwonensis sp. nov., isolated from the rhizosphere of chinese cabbage (brassica campestris).a mesophilic bacterium, strain 15-52(t), was isolated from the rhizosphere of chinese cabbage (brassica campestris). on the basis of phenotypic and genotypic characteristics, the bacterium was identified as representing a novel species belonging to the genus pedobacter. the strain is non-flagellated, non-spore-forming and grows at temperatures in the range 1-37 degrees c. physiological tests of the strain showed the presence of oxidase, catalase, protease (gelatin and casein hydrolysis), beta-gl ...200717329772
effects of soil properties on heavy metal accumulation in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) in pearl river delta, china.this study was conducted to investigate the effects of soil properties on the heavy metal accumulation in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) at the field scale. the concentrations of cadmium (cd), mercury (hg), and chromium (cr) in topsoil and vegetable samples from nanhai district of foshan city in the pearl river delta (prd) were analyzed. the results showed that 56.5% of the soil samples exceeded the grade ii of the chinese soil environme ...200717365337
bcmf13, a new reproductive organ-specific gene from brassica rapa. ssp. chinensis, affects pollen development.a transcript-derived fragment (genbank accession number dn237920.1) accumulated in the wild-type flower buds of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) was isolated and further investigated. the full length dna and cdna of the fragment were cloned by rapid amplification of cdna ends. the gene, bcmf13, encodes a protein of 73 amino acids and is interrupted by an intron of 106 bp (genbank accession number ef158459). southern blot analysis reveale ...200817387632
characterization of 1-aminocyclopropane-1-carboxylate (acc) deaminase containing methylobacterium oryzae and interactions with auxins and acc regulation of ethylene in canola (brassica campestris).the possible interaction of the plant hormones auxin and ethylene and the role of 1-aminocyclopropane-1-carboxylate (acc) deaminase containing bacteria on ethylene production in canola (brassica campestris) in the presence of inhibitory concentrations of growth regulators were investigated. the effects of auxin (indole-3-acetic acid and 2,4-dichlorophenoxy acetic acid), auxin transport inhibitor 2-(p-chlorophenoxy)-2-methylpropionic acid, ethylene precursor 1-aminocyclopropane-1-carboxylate and ...200717541630
[molecular cloning and characterization of bcmybogu, a novel member of the myb family involved in ogucms in brassica campestris ssp. chinensis].in the attempt to elucidate the molecular mechanism of cms. ogura cytoplasmic male sterile (ogucms) lines were obtained in chinese cabbage after interspecific hybridization between brassica. napus l. ogucms and b. campestris ssp. chinensis followed by recurrent backcross with b. campestris ssp. chinensis as the pollen donor. the cms lines were significantly characterized by the whitish anther and indehiscence of anther. the tapetal hypertrophy with excess vacuola-tion was the first observed defe ...200717548334
purification and characterization of an antifungal protein, c-fkbp, from chinese cabbage.an antifungal protein was isolated from chinese cabbage (brassica campestris l. ssp. pekinensis) by buffer-soluble extraction and two chromatographic procedures. the results of matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry revealed that the isolated chinese cabbage protein was identical to human fk506-binding protein (fkbp). a cdna encoding fkbp was isolated from a chinese cabbage leaf cdna library and named c-fkbp. the open reading frame of the gene en ...200717550265
sequence variation of chalcone synthase gene in a spontaneous white-flower mutant of chinese cabbage-pak-choi.a spontaneous white-flower mutant of chinese cabbage-pak-choi (brassica campestris ssp. chinenesis, syn. b. rapa ssp. chinenesis) was found in our test fields, and all the plant characters except flower color were identical with wild type ones. we hypothesized that a mutational event had occurred in the gene coding for chalcone synthase (chs), the key enzyme of flavonoid biosynthesis pathway. two genes, later designated bcchs and bcchs-wf, were isolated from wild type and mutant chinese cabbage- ...200817605003
a steroid fraction of chloroform extract from bee pollen of brassica campestris induces apoptosis in human prostate cancer pc-3 cells.bee pollen of brassica campestris l. is widely used in china as a natural food supplement and an herbal medicine in strengthening the body's resistance against diseases including cancer. the present study was carried out to investigate the effect of a steroid fraction of chloroform extract from bee pollen of brassica campestris l. on human cancer cell viability. our studies show that among nine cancer cell lines of different origin (pc-3, lncap, mcf-7, hela, bel-7402, bcg-823, kb, a549 and ho891 ...200717639562
characterization of a novel gene, bcmf7, that is expressed preferentially in pollen of brassica campestris l. ssp. chinensis makino.pollen formation is important for plant sexual reproduction. to identify the genes that are involved in pollen formation, we performed the genome-wide transcriptional profiling in the flower buds of both male meiotic cytokinesis (mmc) mutant and its wild-type plants of brassica campestris l. ssp. chinensis, syn. b. rapa l. ssp. chinensis. cdna-amplified fragment length polymorphism (cdna-aflp) analysis showed that the mmc mutation resulted in changes in expression of a variety of genes. bcmf7, a ...200717653671
effectiveness and stability of heterologous proteins expressed in plants by turnip mosaic virus vector at five different insertion sites.the n-terminal (nt) regions of particular protein-coding sequences are generally used for in-frame insertion of heterologous open reading frames (orfs) in potyviral vectors for protein expression in plants. an infectious cdna clone of turnip mosaic virus (tumv) isolate yc5 was engineered at the generally used nt regions of hc-pro and cp, and other possibly permissive sites to investigate their effectiveness to express the gfp (jellyfish green fluorescent protein) and der p 5 (allergen from the d ...200717689817
first isolation of an antifungal lipid transfer peptide from seeds of a brassica species.an antifungal peptide with a molecular mass of 9412 and an n-terminal sequence exhibiting notable homology to those of lipid transfer proteins was isolated from seeds of the vegetable brassica campestris. the purification protocol entailed ion exchange chromatography on q-sepharose, affinity chromatography on affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (fplc) on mono s, and gel filtration by fplc on a superdex peptide column. the antifungal peptide was ad ...200717692430
decreased toxicity to terrestrial plants associated with a mixture of methyl tert-butyl ether and its metabolite tert-butyl alcohol.the influence of the main fuel oxygenate methyl tert-butyl ether (mtbe) and its key metabolite, tert-butyl alcohol (tba), on the growth of a plant seedling was studied separately and in combination. the test plants were mung bean (phaseolus radiatus), cucumber (cucumis sativus), wheat (triticum aestivum), sorghum (sorghum bicolor), kale (brassica alboglabra), chinese cabbage (brassica campestris), and sweet corn (zea mays). the growth of all the plants was adversely affected by tba and mtbe. the ...200717702346
lipid transfer proteins from brassica campestris and mung bean surpass mung bean chitinase in exploitability.antifungal peptides with a molecular mass of 9 kda and an n-terminal sequence demonstrating remarkable similarity to those of nonspecific lipid transfer proteins (nsltps) were isolated from seeds of the vegetable brassica campestris and the mung bean. the purified peptides exerted an inhibitory action on mycelial growth in various fungal species. the antifungal activity of brassica and mung bean nsltps were thermostable, ph-stable, and stable after treatment with pepsin and trypsin. in contrast, ...200717726719
brassica campestris l.: floral induction by one long day.a strain of brassica campestris l. responds to a single photoinductive cycle 4 days after sowing. extending the photoperiod from 8 to 22 or 24 hours, with incandescent light of 538-lux intensity, induced inflorescence in 90 percent of the plants. inflorescence development was visible on dissection 5 or 6 days after photoinduction. floral induction increased with duration and intensity of the supplementary light.196617737594
characterization of a putative pollen-specific arabinogalactan protein gene, bcmf8, from brassica campestris ssp. chinensis.the bcmf8 (brassica campestris male fertility 8) gene, possessing the features of 'classical' arabinogalactan protein (agp) was isolated from brassica campestris l. ssp. chinensis, makino syn. b. rapa l. ssp. chinensis. this gene was highly abundant in the fertile flower buds but silenced in the sterile ones of genic male sterile a/b line ('zubajh97-01a/b') in b. campestris. expression patterns analysis suggested bcmf8 was a pollen-specific gene, whose transcript started to be expressed at the u ...200817786584
ozone affects gas exchange, growth and reproductive development in brassica campestris (wisconsin fast plants).exposure to ozone (o(3)) may affect vegetative and reproductive development, although the consequences for yield depend on the effectiveness of the compensatory processes induced. this study examined the impact on reproductive development of exposing brassica campestris (wisconsin fast plants) to ozone during vegetative growth. plants were exposed to 70 ppb ozone for 2 d during late vegetative growth or 10 d spanning most of the vegetative phase. effects on gas exchange, vegetative growth, repro ...200717803646
identification of flavonoids and hydroxycinnamic acids in pak choi varieties (brassica campestris l. ssp. chinensis var. communis) by hplc-esi-msn and nmr and their quantification by hplc-dad.twenty-eight polyphenols (11 flavonoid derivatives and 17 hydroxycinnamic acid derivatives) were detected in different cultivars of the chinese cabbage pak choi ( brassica campestris l. ssp. chinensis var. communis) by hplc-dad-esi-ms(n). kaempferol was found to be the major flavonoid in pak choi, glycosylated and acylated with different compounds. smaller amounts of isorhamnetin were also detected. a structural determination was carried out by (1)h and (13)c nmr spectroscopy for the main compou ...200717848079
coprinellus curtus (hitoyo-take) prevents diseases of vegetables caused by pathogenic fungi.a strain of coprinellus curtus (designated gm-21), a basidiomycete that suppressed bottom-rot disease of chinese cabbage, 'pak-choi' (brassica campestris), caused by the pathogen rhizoctonia solani pak-choi 2 was isolated. the mechanism of plant disease suppression was discovered to be hyphal interference, a combative fungal interaction between strain gm-21 and the pathogen. the antifungal spectrum of strain gm-21 was shown to include r. solani and fusarium sp., i.e. strain gm-21 showed disease- ...200717850327
enhanced tolerance to sulfur dioxide and salt stress of transgenic chinese cabbage plants expressing both superoxide dismutase and catalase in chloroplasts.to explore the possibility of overcoming the highly phytotoxic effect of so(2) and salt stress, we introduced the maize cu/znsod and/or cat genes into chloroplasts of chinese cabbage (brassica campestris l. ssp. pekinensis cv. tropical pride) (referred to as sod, cat and sod+cat plants). sod+cat plants showed enhanced tolerance to 400 ppb so(2), and visible damage was one-sixth that of wild-type (ck) plants. in addition, when sod+cat plants were exposed to a high salt treatment of 200 mm nacl fo ...200717851086
identification and preliminary analysis of a new pcp promoter from brassica rapa ssp. chinensis.the promoter of brassica campestris male fertile 5 (bcmf5), a pollen coat protein member, class a (pcp-a) gene family, was isolated from brassica rapa l. ssp. chinensis makino (chinese cabbage-pak-choi) by thermal asymmetric interlaced polymerase chain reaction (tail-pcr). sequence analysis suggested that the 605-bp promoter of bcmf5 appears to be a pollen promoter. in an attempt to confirm the promoter activity of bcmf5 promoter, -609 to +3 bp and -377 to +3 bp fragments of the upstream sequenc ...200817851779
[genetic model analysis of leaf-weight traits in non-heading chinese cabbage (brassica campestris ssp. chinensis makino)].the mixed major-gene plus polygene inheritance model was used to analyze the genetics of leafblade-weight and petiole-weight trait in sixqiu017 of non-heading chinese cabbage. the results showed that the leafblade-weight trait was controlled by one pair of negative complete dominant major genes plus additive-dominant polygene (d-4). the additive effect in the major gene was 1.8991, the dominant effect was -1.8991; the additive effect in the polygene was -1.2934, and the dominant effect was 1.793 ...200717855268
characterization and functional analysis of a novel pcp gene bcmf5 from chinese cabbage (brassica campestris l. ssp. chinensis makino).brassica campestris male fertile 5 (bcmf5), a novel member of the pollen coat protein class a (pcp-a) gene family, was identified from brassica campestris l. ssp. chinensis makino (chinese cabbage-pak-choi). temporal and spatial expression analysis showed that bcmf5 is a late-expressed pcp gene related to the process of determining pollen fertility. functional analysis by hairpin rna (hprna)-mediated rna interference also showed that the expression of bcmf5 is inhibited, which resulted in the lo ...200817945379
the ecology of bacillus thuringiensis on the phylloplane: colonization from soil, plasmid transfer, and interaction with larvae of pieris brassicae.seedlings of clover (triflorium hybridum) were colonized by bacillus thuringiensis when spores and seeds were co-inoculated into soil. both a strain isolated in the vegetative form from the phylloplane of clover, 2810-s-4, and a laboratory strain, hd-1, were able to colonize clover to a density of about 1000 cfu/g leaf when seeds were sown in sterile soil and to a density of about 300 cfu/g leaf in nonsterile soil. a strain lacking the characteristic insecticidal crystal proteins produced a simi ...200817973155
removal of carotene-like colored compounds by liquid-liquid extraction during polycyclic aromatic hydrocarbons analysis of plant tissue.plants contain a wide variety of chemicals, some of which may have similar chromatographic behavior to polycyclic aromatic hydrocarbons (pahs). during solid phase extraction (spe) with si-gel for instance, the co-elution of carotene-like colored compounds with pahs has been observed. in this paper, liquid-liquid extraction was applied for the separation and subsequent analysis of pahs from plant extracts. pahs containing 2-6 rings, which include naphthalene, phenanthrene, pyrene, benzo[a]pyrene ...200818001818
characterization of a male sterile related gene bcmf15 from brassica campestris ssp. chinensis.data from cdna-aflp analysis based on the genome-wide transcriptional profiling on the flower buds of the male meiotic cytokinesis (mmc) mutant and its wild-type of brassica campestris l. ssp. chinensis makino, syn. b. rapa l. ssp. chinensis, indicated that mutation of the mmc gene resulted in changes in expression of a variety of genes. a transcript-derived fragment specifically accumulated in the wild-type flower buds was isolated, and the corresponding full-length cdna and dna was subsequentl ...200918034318
[study on the spectrum response of brassica campestris l leaf to the zinc pollution].in the present paper, the spectrum response of brassica campestris l leaf to the stress of heavy metal zinc pollution was studied in three spectral rangess of the red edge position (680-740 nm), the visible spectrum (460-680 nm) and the near infrared spectrum (750-1000 nm). the results indicate that the zn content in cabbage leaves increases and the chlorophyll level reduces with the increase in zn concentration in soil. with the zn content of brassica campestris l leaves increasing, the leaf sp ...200718051532
impact of fermentation on phenolic compounds in leaves of pak choi (brassica campestris l. ssp. chinensis var. communis) and chinese leaf mustard (brassica juncea coss).four different cultivars of chinese brassica vegetables (two pak choi cultivars and two chinese leaf mustard cultivars) were fermented according to a traditional chinese method called pickling. the plant material was investigated before and after the fermentation procedure to determine the qualitative and quantitative changes in its polyphenols. a detailed description of the identified phenolic compounds of leaf mustard by hplc-esi-ms(n) is presented here for the first time, including hydroxycin ...200818078315
pyrimidinyl-substituted amides and thioureas: syntheses, crystal structure and herbicidal activities.the high herbicidal activities of [1,2,4]triazolo[1,5-c]pyrimidine and 2h-1,2,4-thiadiazolo[2,3-a]pyrimidine derivatives suggested the development of new fused heterocyclic compounds for application as herbicides.200818080290
composition of the phyllospheric microbial populations on vegetable plants with different glucosinolate and carotenoid compositions.the plant phyllosphere is intensely colonized by a complex and highly diverse microbial population and shows pronounced plant-species-specific differences. the mechanisms and influencing factors determining whether and in which density microorganisms colonize plant phyllosphere tissues are not yet fully understood. one of the key influencing factors is thought to be phytochemical concentration and composition. therefore, correlations between various concentrations of individual glucosinolates an ...200818183453
effects of oyster shell on soil chemical and biological properties and cabbage productivity as a liming materials.oyster shell, a byproduct of shellfish-farming in korea and containing a high amount of caco(3), has a high potential to be used as a liming material in agriculture. however, the agricultural utilization of oyster shell is limited due to its high concentration nacl. the oyster-shell meal collected had a low concentration of water soluble nacl (mean 2.7 g kg(-1)), which might be a result of stacking the material for 6 months in the open field. it has a very similar liming potential with calcium c ...200818294833
combined effect of ultraviolet-b radiation and cadmium contamination on nutrient uptake and photosynthetic pigments in brassica campestris l. seedlings.environmental and industrial pollution along with increase in ground level uv-b radiation, because of stratospheric ozone depletion, present multiple stresses, which may affect crop photosynthesis and productivity. the present study was undertaken to see interactive effects of heavy metal contamination (cd(2+)) and uv-b exposure on essential nutrient (ca(2+), mg(2+), k(+)) uptake, biomass, and chlorophyll content in mustard (brassica campestris l.) seedlings. plants grown in 0.5, 1.0, 2.5, and 5 ...200818348293
functional analysis of a pollen-expressed polygalacturonase gene bcmf6 in chinese cabbage (brassica campestris l. ssp. chinensis makino).in our earlier work, a pollen-expressed polygalacturonase gene bcmf6 was isolated from floral bud of chinese cabbage (brassica campestris l. ssp. chinensis makino) by cdna-amplified fragment length polymorphism (cdna-aflp) transcript profiling and rapid amplification of cdna ends (race). to unravel the biological function of bcmf6 gene, the antisense fragment from the bcmf6 gene with a9 promoter and camv35s promoter was transferred into flowering chinese cabbage (b. campestris ssp. chinensis var ...200818415101
protective effect of the cruciferous vegetable mustard leaf (brassica campestris) against in vivo chromosomal damage and oxidative stress induced by gamma-radiation and genotoxic chemicals.we evaluated the possible protective effect of the popular indian cruciferous vegetable mustard leaf (brassica campestris) against chromosomal damage and oxidative stress induced by gamma-radiation, cyclophosphamide (cph) and urethane (ure), in mice. in vivo bone marrow micronucleus test was performed to assess chromosomal damage, and oxidative stress was monitored by estimating the changes in lipid peroxidation and the status of glutathione (gsh) as well as redox cycle antioxidants. pretreatmen ...200818418865
transcriptional differences between the male-sterile mutant bcms and wild-type brassica campestris ssp. chinensis reveal genes related to pollen development.a novel male-sterile mutant which lacks mature pollen, brassisa campestris male sterile (bcms), was identified in brassica campestris l. ssp. chinensis makino (syn. b. rapa ssp. chinensis). genetic analysis revealed that bcms was controlled by a single recessive mutation locus. genome-wide transcriptional profiling was performed on the flower buds of both the bcms mutant and the wild-type from which it originated, and profiling analysis indicated that there were numerous changes in gene expressi ...200818426481
meiotic studies on a brassica campestris-alboglabra monosomic addition line and derived b. campestris primary trisomics.diakinesis chromosomes were studied in pollen mother cells of brassica campestris (2n = 20, genome aa), b. alboglabra (2n = 18, genome cc), a b. campestris-alboglabra monosomic addition line (aa + 1 chromosome from the c genome), and four derived b. campestris primary trisomics. the nucleolar chromosomes of b. campestris were distinguishable by their morphology at diakinesis. the alien c-genome chromosome in the addition line paired preferentially with the nucleolar chromosome of the a genome. v ...199418470102
mitotic karyotypes of brassica campestris and brassica alboglabra and identification of the b. alboglabra chromosome in an addition line.a brassica campestris-alboglabra monosomic addition line (genome: aa + one chromosome from the c genome, 2n = 21) harbours the brassica alboglabra (cc, 2n = 18) chromosome with the gene for erucic acid. in order to identify this chromosome, we have studied the mitotic prometaphase chromosomes of brassica campestris (aa, 2n = 20), b. alboglabra, and the monosomic addition line. more pronounced differential staining and size differences of chromosomes were observed in b. campestris than in b. albo ...199518470170
erratum: mitotic karyotypes of brassica campestris and brassica alboglabra and identification of the b. alboglabra chromosome in an addition line. 199518470209
ethnoveterinary practices for the treatment of parasitic diseases in livestock in cholistan desert (pakistan).this study was conducted to document the ethnoveterinary medicinal (evm) practices for the treatment of different parasitic diseases of livestock in cholistan desert, pakistan.200818524514
quadratic form: a robust metric for quantitative comparison of flow cytometric histograms.comparison of fluorescence distributions is a fundamental part of the analysis of flow cytometric data. this approach is applied to detect differences between control and test sample and thus analyze a biological response. comparison of standard test samples over time provides an estimate of instrument stability for quality control. however, application of statistical methods of distribution comparison in flow cytometry is difficult owing to instrument noise and the complex shape of intensity di ...200818561196
morphological and functional characterization of bcmf13 in the antisense-silenced plants of brassica campestris ssp. chinensis var. parachinensis.the gene brassica campestris male fertility 13 (bcmf13, genbank accession number ef158459) was isolated as a reproductive organ-specific gene from chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis). it is exclusively expressed in stage four and five flower buds of fertile lines and is most strongly expressed in stamens. here, we report a functional characterization of this bcmf13 gene in the antisense-silenced plants. the inflorescence of the bcmf13 mutan ...200918592396
accumulation and distribution of toxic metals in wheat (triticum aestivum l.) and indian mustard (brassica campestris l.) irrigated with distillery and tannery effluents.in the present study, accumulation and distribution of toxic metals (cu, cd, cr, zn, fe, ni, mn, and pb) and their biochemical effect on wheat and mustard plants irrigated with mixed distillery and tannery effluents are reported. analyses of effluents and soil samples have shown high metal content than the permissible limit except pb. further, analyses of plant samples have indicated the maximum accumulation of fe (340 mg kg(-1) in wheat root and 560 mg kg(-1) in mustard leaves) followed by mn a ...200918650002
[effects of silicon on flowering chinese cabbage's anthracnose occurence, flower stalk formation, and silicon uptake and accumulation].different concentrations of silicon (si) were applied to flowering chinese cabbbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) to study their effects on the flowering chinese cabbage's anthracnose occurrence, flower stalk formation, and si uptake and accumulation. the results indicated that si could obviously control the occurrence of anthracnose, and the effect was genotype-dependant. the plants of susceptible cultivar applied with 2.5 mmol l(-1) si and those of resistant c ...200818655585
an account of the botanical anthelmintics used in traditional veterinary practices in sahiwal district of punjab, pakistan.the present study was aimed at documentation of botanical anthelmintics used in the traditional veterinary practices in sahiwal district of punjab, pakistan.200818657602
phytoextraction of zinc, copper, nickel and lead from a contaminated soil by different species of brassica.in a pot culture experiment, five different species of brassica (brassica juncea, brassica campestris, brassica carinata, brassica napus, and brassica nigra) were grown for screening possible accumulators of heavy metals, viz. zn, cu, ni, and pb. the plants were grown to maturity in a soil irrigated with sewage effluents for more than two decades in west delhi, india. the soil analysis showed enhanced accumulation of zn, cu, ni, and pb in this sewage-irrigated soil. among all species, b. carinat ...201318709932
[the pta29-barnase chimeric gene transformation of brassica campestris l. subsp. chinensis makino var. parachinensis mediated by agrobacterium].in order to induce male sterility of brassica campestris l. subsp. chinensis makino var. parachinensis, we introduced the chimeric pta29-barnase gene into it by agrobacteriumtume faciens transformation. we obtained the transgenic plants, and determined them by pcr, southern blotting and rt-pcr analysis. results indicated that the rnase (barnase) gene had been transferred into genome of plant, and its expression level was different among transformation plants. all transgenic plants were male ster ...200818724712
effects of bt transgenic chinese cabbage on the herbivore mamestra brassicae (lepidoptera: noctuidae) and its parasitoid microplitis mediator (hymenoptera: braconidae).we investigated the effects of a diamondback moth-resistant chinese cabbage (brassica campestris subsp napus variety pekinensis makino), expressing the insecticidal protein cryla(c) toxin derived from bacillus thuringiensis, on the nontarget herbivore mamestra brassicae (l.) (lepidoptera: noctuidae) and its parasitoid wasp microplitis mediator (haliday) (hymenoptera: braconidae). a decreased survival rate at neonate stage was observed in m. brassicae when reared on bt cabbage, although overall d ...200818767720
chinese cabbage (brassica campestris l.) does not improve glucose tolerance, serum insulin, or blood lipid profiles in a rat model of type-2 diabetes.the present study was conducted to investigate the effects of a low (0.5%) and a high (2.0%) dietary dose of freeze-dried chinese cabbage (cc) (brassica campestris l.) powder in a type-2 diabetes (t2d) model of rats. five-week-old male sprague-dawley rats were fed a high fat (hf)-containing diet for 2 wk then randomly divided into 4 groups of 8 animals, namely: normal control (nc), diabetic control (dbc), chinese cabbage low (ccl, 0.5%), and chinese cabbage high (cch, 2.0%) groups. diabetes was ...200819021803
the polygalacturonase gene bcmf2 from brassica campestris is associated with intine development.brassica campestris male fertility 2 (bcmf2) is a putative polygalacturonase (pg) gene previously isolated from the flower bud of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis). this gene was found to be expressed specifically in tapetum and pollen after the tetrad stage of anther development. antisense rna technology was used to study the function of bcmf2 in chinese cabbage. scanning and transmission electron microscopy revealed that there were defo ...200819039102
evaluation of physiological, growth and yield responses of a tropical oil crop (brassica campestris l. var. kranti) under ambient ozone pollution at varying npk levels.a field study was conducted to evaluate the impact of ambient ozone on mustard (brassica campestris l. var. kranti) plants grown under recommended and 1.5 times recommended npk doses at a rural site of india using filtered (fcs) and non-filtered open top chambers (nfcs). ambient mean o(3) concentration varied from 41.65 to 54.2ppb during the experiment. plants growing in fcs showed higher photosynthetic rate at both npk levels, but higher stomatal conductance only at recommended npk. there were ...200919070410
the lcb2 subunit of the sphingolip biosynthesis enzyme serine palmitoyltransferase can function as an attenuator of the hypersensitive response and bax-induced cell death.previous results showed that expression of the gene encoding the long-chain base2 (lcb(2)) subunit of serine palmitoyltransferase (spt), designated bclcb(2), from nonheading chinese cabbage (brassica campestris ssp. chinensis) was up-regulated during hypersensitive cell death (hcd) induced by the phytophthora boehmeriae elicitor pb90. overexpression of bclcb(2) in nicotiana tabacum leaves suppressed the hcd normally initiated by elicitors and pb90-triggered h(2)o(2) accumulation. bclcb(2) also f ...200919076721
[toxicological effects of omethoate on leaf photosystem ii of cole].after spraying different concentrations of two brands pesticide omethoate on cole (brassica campestris l.) leaves, the leaf chlorophyll a fluorescence transients were measured by a plant efficiency analyzer (pea), and the toxicological effects and rudimental dynamic courses of omethoate on the leaf photosystem ii (ps ii) were investigated by jip-test. the results showed that after spraying omethoate except at the concentration of 0.50% , the maximal efficiency of photochemistry (f(v)/f(m)) did n ...200819123372
selective separation of peptides contained in a rapeseed (brassica campestris l.) protein hydrolysate using uf/nf membranes.the ability of a charged uf membrane to fractionate the small peptides found in a rapeseed protein enzymatic hydrolysate, according to their charge characteristics, was investigated. the complexity of such a hydrolysate has required the setting up of technological alternatives to isolate the small peptides, to obtain a more efficient separation among the numerous peptide species. a preliminary step consisted of precipitation followed by filtration with a 3000 g/mol molecular weight cutoff (mwco) ...200619127728
molecular cloning and characterization of a novel pollen predominantly membrane protein gene bcmf12 from brassica campestris ssp. chinensis.a novel membrane protein gene, bcmf12, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino) using rapid amplification of the cdna ends based on a pollen-specific cdna fragment (dn237936). the cdna was 1,155 bp in length with an open reading frame of 894 bp capable of encoding a putative polypeptide of 297 amino acids with an estimated molecular mass of 34.6 kda and a predicted isoelectric point of 9.6. comparative and bioinformatics analyses revealed that bcmf12 showe ...200919169847
aromatase inhibitory fatty acid derivatives from the pollen of brassica campestris l. var. oleifera dc.two new fatty acid derivatives, 9z,12z,15z-octadecatrienoic acid sorbitol ester (1) and (10,11,12)-trihydroxy-(7z,14z)-heptadecadienoic acid (2), were isolated from the pollen of brassica campestris l. var. oleifera dc., along with the four known fatty acid derivatives, 9z,12z,15z-octadecatrienamide, n-(2-hydroxyethyl) (3), hexadecanoic acid sorbitol ester (4), 15,16-dihydroxy-9z,12z-octadecadienoic acid (5), and 9z,12z,15z-octadecatrienoic acid 2,3-dihydroxypropyl ester (6). their structures we ...200919219725
effect of bacillus thuringiensis naturally colonising brassica campestris var. chinensis leaves on neonate larvae of pieris brassicae.the feeding of neonate larvae of pieris brassicae (order lepidoptera) on leaves of brassica plants that had been colonised by bacillus thuringiensis resulted in the death of 35% of the population within 72h. the bacteria multiplied in the cadavers, resulting in an increase of about 50-fold compared to the living insects. surviving insects showed no ill effects during the time of the study. there was negligible multiplication of b. thuringiensis in the frass.200919232351
reducing plant uptake of pahs by cationic surfactant-enhanced soil retention.reducing the transfer of contaminants from soils to plants is a promising approach to produce safe agricultural products grown on contaminated soils. in this study, 0-400 mg/kg cetyltrimethylammonium bromide (ctmab) and dodecylpyridinium bromide (ddpb) were separately utilized to enhance the sorption of pahs onto soils, thereby reducing the transfer of pahs from soil to soil solution and subsequently to plants. concentrations of phenanthrene and pyrene in vegetables grown in contaminated soils t ...200919232798
over-expression of putative transcriptional coactivator kelp interferes with tomato mosaic virus cell-to-cell movement.tomato mosaic virus (tomv) encodes a movement protein (mp) that is necessary for virus cell-to-cell movement. we have demonstrated previously that kelp, a putative transcriptional coactivator of arabidopsis thaliana, and its orthologue from brassica campestris can bind to tomv mp in vitro. in this study, we examined the effects of the transient over-expression of kelp on tomv infection and the intracellular localization of mp in nicotiana benthamiana, an experimental host of the virus. in co-bom ...200919236566
screening brassica species for glucosinolate content.glucosinolates (gsls), a group of compounds found in brassica plants, are toxic to some soil-borne plant pathogens because of the toxicity of their hydrolysis products, isothiocyanates. other phytochemicals found in brassica plants, such as phenols and ascorbic acid, may compliment the activity of gsls. a survey of brassica accessions from the national germplasm repository was conducted to identify potential cover crops that could be soil-incorporated for use as biofumigants. ten brassica access ...200919280485
impact of sewage irrigation on speciation of nickel in soils and its accumulation in crops of industrial towns of punjab.analysis of soil samples collected from sewage and tube well irrigated soils of ludhiana, amritsar jalandhar and mandi gobindgarh, revealed that diethylene triamine pentaacetic acid extractable nickel ( dtpa-ni) was found to be higher in sewage fed soils. sewage irrigation increased soil dtpa-ni content by 3.04 times over the tube well irrigated soils. the content of dtpa-ni showed decreasing trend with depth. hydrogen concentration (ph) was negatively and significantly correlated with dtpa-ni n ...200819295085
isolation and characterization of juncin, an antifungal protein from seeds of japanese takana (brassica juncea var. integrifolia).an 18.9 kda antifungal protein designated juncin was isolated from seeds of the japanese takana (brassica juncea var. integrifolia). the purification protocol employed comprised anion-exchange chromatography on q-sepharose, affinity chromatography on affi-gel blue gel, cation exchange chromatography on sp-sepharose, and gel filtration on superdex 75. juncin was adsorbed on affi-gel blue gel and sp-sepharose but unadsorbed on q-sepharose. the protein exhibited antifungal activity toward the phyto ...200919354248
using matrix solid-phase microextraction (matrix-spme) to estimate bioavailability of ddts in soil to both earthworm and vegetables.this study was conducted to find an appropriate approach for the assessment of bioavailability of ddts in soil to both earthworm and vegetables. four chemical approaches--soxhlet extraction with n-hexane, n-butanol agitation extraction, water agitation extraction, and matrix solid-phase microextraction (matrix-spme)--were used to assess the relationships between the extractability of 1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene (p,p'-dde), 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) e ...201019418090
dielectric spectroscopy of plant protoplasts.the relative permittivity and conductivity of the mesophyll protoplasts isolated from brassica campestris leaves and tulipa gesneriana petals were measured over a frequency range from 1khz to 500 mhz.these protoplasts showed a broad dielectric dispersion, which was composed of three subdispersions, termed beta(1)-, beta(2)-, and beta(3)-dispersion in increasing order of frequency.the three subdispersions were assigned to the maxwell-wagner dispersion caused by charging processes at the interface ...199219431864
residue analysis of acephate and its metabolite methamidophos in open field and greenhouse pakchoi (brassica campestris l.) by gas chromatography-tandem mass spectrometry.to analyze the dynamic degradation and final residues of acephate and its metabolite methamidophos, field-experiments with pakchoi (brassica campestris l.) in open field and greenhouse were carried out in beijing, china in 2004 and 2005. the degradation dynamics and final residues were determined by gas chromatography (gc) equipped with a pulsed flame photometric detector and gc coupled to mass spectrometry (ms)/ms after acephate was applied on open field and green house pakchoi (b. campestris l ...201019496002
eco-toxic effects of sulfadiazine sodium, sulfamonomethoxine sodium and enrofloxacin on wheat, chinese cabbage and tomato.investigation of the toxic effects of three veterinary drugs [sulfadiazine sodium (sds), sulfamonomethoxine sodium (smms), and enrofloxacin (eflx)] on seed germination, root elongation and shoot elongation of wheat (triticum aestivum l.), chinese cabbage (brassica campestris l.) and tomato (cyphomandra betacea) was carried out. significant linear relationships between the root and shoot elongation and the concentration of veterinary drugs addition were observed. the effects of the three veterina ...200919554446
interaction between plants and bacteria: glucosinolates and phyllospheric colonization of cruciferous vegetables by enterobacter radicincitans dsm 16656.for determining interactive plant-bacterial effects between glucosinolates and phyllospheric colonization by a plant growth-promoting strain, enterobacter radicincitans dsm 16656, in cruciferous vegetables, the extent of bacterial colonization was assessed in 5 cruciferous vegetables (brassica juncea, brassica campestris, brassica oleracea var. capitata, brassica rapa var. alboglabra, nasturtium officinale) using a species-specific taqman probe and quantitative real-time pcr. colonization abilit ...200919556746
campesin, a thermostable antifungal peptide with highly potent antipathogenic activities.an 9.4-kda antifungal peptide designated as campesin was isolated from seeds of the cabbage brassica campestris. the isolation procedure involved affinity chromatography on affi-gel blue gel, ion exchange chromatography on q-sepharose and mono s, and gel filtration on superdex 75 and superdex peptide. the peptide was adsorbed on the first three chromatographic media. it exerted an inhibitory action on mycelial growth including fusarium oxysporum and mycosphaerella arachidicola, with an ic(50) of ...200919664563
the construction of a genetic linkage map of non-heading chinese cabbage (brassica campestris ssp. chinensis makino).non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is one of the most important vegetables in eastern china. a genetic linkage map was constructed using 127 doubled haploid (dh) lines, and the dh population was derived from a commercial hybrid "hanxiao" (lines sw-13 x l-118). out of the 614 polymorphic markers, 43.49% were not assigned to any of the linkage groups(lgs). chi-square tests showed that 42.67% markers were distorted from expected mendelian segregation ratios, and ...200919683673
bcmf9, a novel polygalacturonase gene, is required for both brassica campestris intine and exine formation.the polygalacturonase (pg) gene family has been found to be enriched in pollen of several species; however, little is currently known about the function of the pg gene in pollen development. to investigate the exact role that the pg gene has played in pollen development and about this family in general, one putative pg gene, brassica campestris male fertility 9 (bcmf9), was isolated from chinese cabbage (brassica campestris ssp. chinensis, syn. b. rapa ssp. chinensis) and characterized.200919815569
differential expression of salt tolerance related genes in brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee.we examined salt tolerance responsive genes in pak-choi under salt stress and analyze their potential function. the mrna differential display was used to screen the transcript derived fragments (tdfs) related to salinity tolerance in tolerant and moderately tolerant pak-choi germplasm. seventy-eight primer combinations generated 101 differential cdna fragments, which were divided into 10 expression types. seven cdna sequences (genbank accession nos. dq006915~dq006921) obtained and sequenced were ...200919882760
ethanolic extracts of brassica campestris spp. rapa roots prevent high-fat diet-induced obesity via beta(3)-adrenergic regulation of white adipocyte lipolytic activity.the influence of ethanolic extracts of brassica campestris spp. rapa roots (ebr) on obesity was examined in imprinting control region (icr) mice fed a high-fat diet (hfd) and in 3t3-l1 adipocytes. the icr mice used were divided into regular diet, hfd, ebr (50 mg/kg/day ebr administered orally), and orlistat (10 mg/kg/day orlistat administered orally) groups. the molecular mechanism of the anti-obesity effect of ebr was investigated in 3t3-l1 adipocytes as well as in hfd-fed icr mice. in the obes ...201020132043
a polygalacturonase inhibitory protein gene (bcmf19) expressed during pollen development in chinese cabbage-pak-choi.the level of polygalacturonase inhibitory protein (pgip) genes involved in pollen development remains unclear. characterization of the different pgip genes that are expressed in pollen is necessary in understanding the similarities and differences of functions between the members of this gene family, as well as the underlying mechanism of pollen development. a gene-encoding putative pgip, bcmf19 was successfully cloned on a cdna-amplified fragment length polymorphism fragment after it was found ...201120373034
diffusion and accumulation in cultivated vegetable plants of di-(2-ethylhexyl) phthalate (dehp) from a plastic production factory.di-(2-ethylhexyl) phthalate (dehp) concentrations in the atmosphere and in four vegetable crops including brassica chinensis l. (bok choy), brassica campestris l. (field mustard), vigna unguiculata walp. (cowpea), and solanum melongena l. (eggplant) cultivated on land surrounding a plastic production factory were determined. the air dehp concentrations (means) at the sites 0.2, 0.4, 0.8, and 1.6 km away from the plastic production building were about 9.4-12.8, 5.8-9.6, 1.6-5.0, and 0.04-0.27 mic ...201020496185
a novel method for constructing pathogen-regulated small rna cdna library.pathogen-responsive endogenous small non-coding rnas regulate gene expression in relation to plant immune responses by serving as rna silencing machinery. decay caused by the bacterium, erwinia carotovora subsp. carotovora (ecc), often leads to soft rot disease in the plant brassica campestris l. ssp. pekinensis (bcp). to discover endogenous small rna species in bcp in response to ecc infection, we developed a highly efficient approach for cloning pathogen-regulated small rnas. a group of degene ...201020515661
role of ca2+ on growth of brassica campestris l. and b. juncea (l.) czern & coss under na+ stress.root and shoot growth of brassica campestris l. and b. juncea increased significantly (p < 0.01) with enhanced ca(2+) treatment along with 60 mm nacl in the root medium. the maximum fresh mass of shoot and root in b. juncea was recorded at 10 mm ca(2+) concentration. the relative growth rate of shoot of both species reached its maximum at 8 mm of ca(2+) concentration. average rate of ca(2+) intake (i(ca)) was higher in b. juncea than b. campestris. in b. juncea, the average transport of ca(2+) t ...201020590985
pollenopyrroside a and b, novel pyrrole ketohexoside derivatives from bee-collected brassica campestris pollen.pollenopyrroside a and pollenopyrroside b, novel pyrrole ketohexoside derivatives, were isolated from the extract of bee-collected brassica campestris pollen. their structures were elucidated by spectroscopic analysis (uv, ir, ms, nmr and x-ray) and chemical evidence. pollenopyrrosides a and b represent a novel carbon skeleton with a six-six and five-six member dioxaspirocycle bearing nitrogen atom, respectively.201020606352
soil and vegetables enrichment with heavy metals from geological sources in gilgit, northern pakistan.this study was conducted to investigate the concentrations of heavy metals in soil and vegetables, and human health risks through ingestion of contaminated vegetables. soil and vegetable samples were collected from different locations in gilgit, northern pakistan, and analyzed for cd, cu, ni, pb and zn. plant transfer factors (ptf), daily intake of metals (dim) and health risk index (hri) were also calculated. the concentrations of cd, cu and zn exceeded their respective permissible limits in so ...201020810165
responses of mikania micrantha, an invasive weed to elevated co₂: induction of β-caryophyllene synthase, changes in emission capability and allelopathic potential of β-caryophyllene.to better understand the effect of predicted elevated levels of carbon dioxide (co₂) on an invasive weed mikania micrantha, we constructed a suppressive subtractive hybridization (ssh) library from the leaves of m. micrantha exposed to co₂ at 350 and 750 ppm for 6 d, and isolated a novel gene named β-caryophyllene synthase. β-caryophyllene synthase catalyses the conversion of farnesyl diphosphate to β-caryophyllene, a volatile sesquiterpene with allelopathic potential. real-time pcr analysis rev ...201020821346
toxicities of fipronil enantiomers to the honeybee apis mellifera l. and enantiomeric compositions of fipronil in honey plant flowers.fipronil is a chiral phenylpyrazole insecticide that is effective for control of a wide range of agricultural and domestic pests at low application rates. wide application of fipronil also causes poisoning of some nontarget insects, such as honeybees. in the present study, toxicities of fipronil enantiomers and racemate to the honeybee apis mellifera l. were determined to examine whether using formulations of single or enriched fipronil enantiomer is a possible option to reduce risks to bees. co ...201020821427
isolation and characterization of a novel bcmf14 gene from brassica campestris ssp. chinensis.a putative ralf (rapid alkalinization factor)-like gene (genbank accession number ef523517), named bcmf14, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) by rapid amplification of cdna ends (race) based on a cdna-aflp differential fragment exclusively expressed in fertile line. semi-quantitative reverse transcription-polymerase chain reaction (rt-pcr) discovered that bcmf14 was prominently expressed in stage four and five flower buds ...201120857218
interaction of 8-hydroxyquinoline with soil environment mediates its ecological function.allelopathic functions of plant-released chemicals are often studied through growth bioassays assuming that these chemicals will directly impact plant growth. this overlooks the role of soil factors in mediating allelopathic activities of chemicals, particularly non-volatiles. here we examined the allelopathic potential of 8-hydroxyquinoline (hq), a chemical reported to be exuded from the roots of centaurea diffusa.201020877629
production of well-matured compost from night-soil sludge by an extremely short period of thermophilic composting.the effect of various operational conditions on the decomposition of organic material during the composting of night-soil treatment sludge was quantitatively examined. the optimum composting conditions were found to be a temperature of ca. 60°c and an initial ph value of 8. rapid decomposition of organic matter ceased by the sixth day of composting under these optimum conditions, and the final value of the cumulative emission of carbon (e(c)), which represents the degree of organic matter decomp ...201121134735
leaching behaviour of pendimethalin causes toxicity towards different cultivars of brassica juncea and brassica campestris in sandy loam soil.an experiment was conducted at the farm of zonal adaptive research station, uttar banga krishi viswavidhyalaya, pundibari, cooch behar, west bengal to evaluate the effect of pendimethalin on the yield, weed density and phytotoxicity in different varieties of rai (brassica juncea) and yellow sarson (b. campestris var. yellow sarson) under higher soil moisture regime in terai region of west bengal. pre-emergence application of pendimethalin at higher dose i.e. 1.0 kg/ha recorded higher plant morta ...200921217862
pedobacter rhizosphaerae sp. nov. and pedobacter soli sp. nov., isolated from the rhizosphere soil of chinese cabbage (brassica campestris).two bacterial strains 01-96(t) and 15-51(t) isolated from the rhizosphere soil of chinese cabbage (brassica campestris), were characterized using a polyphasic approach. the 16s rrna gene sequence analysis showed that strain 01-96(t) was phylogenetically related to pedobacter suwonensis 15-52(t) and pedobacter roseus 25b(t) (96.7 % and 96.7 % sequence similarity, respectively), and strain 15-51(t) was closely related to pedobacter borealis tfb(t) and pedobacter suwonensis 15-52(t) (97.2 % and 97. ...201121257686
bcmf20, a putative pollen-specific transcription factor from brassica campestris ssp. chinensis.the arabidopsis transcription factor (at1g26610) was shown to be down-regulated in male sterile lines compared to their maintainer line in chinese cabbage (brassica campestris ssp. chinensis) in our previous study. the bcmf20 gene, homologue of the at1g26610 gene was isolated from chinese cabbage. it encodes a putative c2h2 zinc finger protein. the spatial and temporal expression patterns examined by qrt-pcr and in situ hybridization, indicated bcmf20 is specifically expressed in the developing ...201121327825
1,8-cineole inhibits both proliferation and elongation of by-2 cultured tobacco cells.volatile monoterpenes such as 1,8-cineole inhibit the growth of brassica campestris seedlings in a dose-dependent manner, and the growth-inhibitory effects are more severe for roots than hypocotyls. the preferential inhibition of root growth may be explained if the compounds inhibit cell proliferation more severely than cell elongation because root growth requires both elongation and proliferation of the constituent cells, whereas hypocotyl growth depends exclusively on elongation of existing ce ...201121344180
growth promotion of chinese cabbage and arabidopsis by piriformospora indica is not stimulated by mycelium-synthesized auxin.piriformospora indica, an endophytic fungus of the order sebacinales, interacts with the roots of a large variety of plant species. we compared the interaction of this fungus with chinese cabbage (brassica campestris subsp. chinensis) and arabidopsis seedlings. the development of shoots and roots of chinese cabbage seedlings was strongly promoted by p. indica and the fresh weight of the seedlings increased approximately twofold. the strong stimulation of root hair development resulted in a bushy ...201121375386
chemical fractionations and bioavailability of cadmium and zinc to cole (brassica campestris l.) grown in the multi-metals contaminated oasis soil, northwest of china.a pot experiment was conducted to study the relationship between distribution of cadmium (cd) and zinc (zn) and their availability. to cole (brassica campestris l.) grown in the multi-metal contaminated oasis soil in northwest of china. the results showed that cd and zn in the unpolluted oasis soil was mainly found in the residual fractionation, however, with increasing contents of cd and zn in the oasis soil, the distribution of cd and zn changed significantly. the growth of cole could be promo ...201121517001
synthesis, structure and biological activity of novel 1,2,4-triazole mannich bases containing a substituted benzylpiperazine moiety.a series of novel mannich bases with trifluoromethyl-1,2,4-triazole and substituted benzylpiperazine moieties were synthesized. their structures were confirmed by ir, (1) h nmr and elemental analysis. the single crystal structure of compound 4r was also determined. the preliminary bioassays showed that most of the lead compounds had low herbicidal activity against brassica campestris, echinochloa crusgalli, and kari enzyme. however, most of them exhibited significant fungicidal activity at the d ...201121521489
[effects of exogenous dimethylarsinic acid on brassica campestris growth and soil arsenic bioavailability].a pot experiment was conducted to study the effects of exogenous dimethylarsinic acid (dma) on the growth of brassica campestris and the bioavailability of soil arsenic (as). with the increasing concentration of applied dma, the emergence rate and biomass of b. campestris increased at low concentration dma, but decreased at high concentration dma. when the dma concentration reached 90 mg x kg(-1), the emergence rate and biomass of b. campestris in the second cropping decreased by 9.5% and 57.0%, ...201121608259
roles of xanthophylls and exogenous aba in protection against nacl-induced photodamage in rice (oryza sativa l) and cabbage (brassica campestris).changes in actual efficiency of ps ii photochemistry, non-photochemical quenching (npq), content of xanthophylls and kinetics of de-epoxidation were studied in aba-fed and non-aba-fed leaves of rice and cabbage under nacl stress. salt stress induced more progressive decrease in actual efficiency of ps ii photochemistry (фps ii), higher reduction state of ps ii, and a small significant increase in npq in nacl-sensitive rice plants as compared with nacl-tolerant cabbage plants, whereas exogenously ...201121642236
analysis of the four cox2 genes found in turnip (brassica campestris, brassicaceae) mitochondria.in turnip mitochondria the cox2 gene is partially contained within a 2-kb (kilobase) direct repeat that is the site of homologous recombination events that generate subgenomic molecules. because of its partial presence within the direct repeat, the turnip cox2 gene exists in four genomic contexts, two on the master chromosome and one on each of the subgenomic circles. in order to gain information on the expression of plant mitochondrial genes that exist in multiple genomic contexts and on plant ...199821684900
characterization of alginase and elicitor-active oligosaccharides from gracilibacillus a7 in alleviating salt stress for brassica campestris l.alginase was purified from gracilibacillus a7 and evaluated for its ability to produce elicitor-active oligosaccharides. the optimum conditions for the alginase reaction are as follows: temperature, 40 -¦c; ph, 8.0; alginate content, 0.3-0.7%; and the presence of na(+) and mg(2+) metal ions. the degree of polymerization (dp) decreased as the reaction time of the alginase progressed, achieving values of 5.4 and 3.3 after 240 and 300 min, respectively. the relative root length (rrl) of the brassic ...201121696216
lead (pb)-inhibited radicle emergence in brassica campestris involves alterations in starch-metabolizing enzymes.lead (pb) is a toxic heavy metal released into the natural environment and known to cause oxidative damage and alter antioxidant mechanism in plants. however, not much is known about the interference of pb with the biochemical processes and carbohydrate metabolism during seed germination. we, therefore, investigated the effect of pb (50-500 μm) upon biochemical alterations in germinating seeds (at 24-h stage) of brassica campestris l. pb treatment significantly enhanced protein and carbohydrate ...201121735112
swede midge (diptera: cecidomyiidae), ten years of invasion of crucifer crops in north america.the swede midge, contarinia nasturtii kieffer (diptera: cecidomyiidae), a common insect pest in europe, is a newly invasive pest in north america that constitutes a major threat to cruciferous vegetable and field crops. since its first identification in ontario, canada, in 2000, it has rapidly spread to 65 counties in the provinces of ontario and quebec and has recently been found in canola (one of two cultivars of rapeseed, brassica napus l. and brassica campestris l.) in the central prairie re ...201121735885
A novel cold-inducible gene from Pak-choi (Brassica campestris ssp. chinensis), BcWRKY46, enhances the cold, salt and dehydration stress tolerance in transgenic tobacco.WRKY TFs belong to one of the largest families of transcriptional regulators in plants and form integral parts of signaling webs that modulate many plant processes. BcWRKY46, a cDNA clone encoding a polypeptide of 284 amino acids and exhibited the structural features of group III of WRKY protein family, was isolated from the cold-treated leaves of Pak-choi (Brassica campestris ssp. chinensis Makino, syn. B. rapa ssp. chinensis) using the cDNA-AFLP technique. Expression of this gene was induced q ...201121938429
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