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comparative transcriptome analysis between low- and high-cadmium-accumulating genotypes of pakchoi (brassica chinensis l.) in response to cadmium stress.to reduce cadmium (cd) pollution of food chains, screening and breeding of low-cd-accumulating cultivars are the focus of much study. two previously identified genotypes, a low-cd-accumulating genotype (lajk) and a high-cd-accumulating genotype (hajs) of pakchoi (brassica chinesis l.), were stressed by cd (12.5 μm) for 0 h (t0), 3 h (t3) and 24 h (t24). by comparative transcriptome analysis for root tissue, 3005 and 4343 differentially expressed genes (degs) were identified in lajk at t3 (vs t0) ...201627228483
improved discrimination for brassica vegetables treated with agricultural fertilizers using a combined chemometric approach.multielement and stable isotope (δ(13)c, δ(15)n, δ(2)h, δ(18)o, (207)pb/(206)pb, and (208)pb/(206)pb) analyses were combined to provide a new chemometric approach to improve the discrimination between organic and conventional brassica vegetable production. different combinations of organic and conventional fertilizer treatments were used to demonstrate this authentication approach using brassica chinensis planted in experimental test pots. stable isotope analyses (δ(15)n and δ(13)c) of b. chinen ...027355562
effects of dissolved organic matter on uptake and translocation of lead in brassica chinensis and potential health risk of pb.dissolved organic matter (dom) can affect the bioavailability of heavy metals in soil, especially in soils used for vegetable production, where intensive organic fertilization is applied. the present study examined the effects of dom derived from commercial organic fertilizers (cof), cow manure (com) and chicken manure (chm), on uptake and translocation of lead (pb) in brassica chinensis in a pot experiment. the results indicate that dom derived from chm (domchm) significantly increased pb conce ...201627399753
effects of an iron-silicon material, a synthetic zeolite and an alkaline clay on vegetable uptake of as and cd from a polluted agricultural soil and proposed remediation mechanisms.economic and highly effective methods of in situ remediation of cd and as polluted farmland in mining areas are urgently needed. pot experiments with brassica chinensis l. were carried out to determine the effects of three soil amendments [a novel iron-silicon material (ism), a synthetic zeolite (sz) and an alkaline clay (ac)] on vegetable uptake of as and cd. sem-eds and xrd analyses were used to investigate the remediation mechanisms involved. amendment with ism significantly reduced the conce ...201727530933
accumulation of cr, cd, pb, cu, and zn by plants in tanning sludge storage sites: opportunities for contamination bioindication and phytoremediation.tanning sludge enriched with high concentrations of cr and other metals has adverse effects on the environment. plants growing in the metalliferous soils may have the ability to cope with high metal concentrations. this study focuses on potentials of using native plants for bioindication and/or phytoremediation of cr-contaminated sites. in the study, we characterized plants and soils from six tanning sludge storage sites. soil in these sites exhibited toxic levels of cr (averaged 16,492 mg kg(-1 ...201627552994
slow-release nitrogen fertilizers can improve yield and reduce cd concentration in pakchoi (brassica chinensis l.) grown in cd-contaminated soil.cadmium (cd) pollution in vegetable crops has become a serious problem in recent years. owing to the limited availability of arable land resources, large areas of cd-contaminated lands are inevitably being used for the production of vegetables, posing great risks to human health via the food chain. however, strategies to improve yield and reduce cd concentration in crops grown in contaminated soils are being developed. in the present study, using pot experiments, we investigated the effects of t ...027677996
characterization of bcmf23a and bcmf23b, two putative pectin methylesterase genes related to pollen development in brassica campestris ssp. chinensis.two homologous genes, brassica campestris male fertility 23a (bcmf23a) and brassica campestris male fertility 23b (bcmf23b), encoding putative pectin methylesterases (pmes) were isolated from brassica campestris ssp. chinensis (syn. brassica rapa ssp. chinensis). these two genes sharing high sequence identity with each other were highly expressed in the fertile flower buds but silenced in the sterile ones of genic male sterile line system ('bcajh97-01a/b'). results of rt-pcr and in situ hybridiz ...201727844189
over-expression of mir158 causes pollen abortion in brassica campestris ssp. chinensis.we identified and cloned the two precursors of mir158 and its target gene in brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. further study revealed that over-expression of mir158 caused reduced pollen varbility, which was caused by the degradation of pollen contents from the binucleate microspore stage. these results first suggest the role of mir158 in pollen development of brassica campestris ssp. chinensis. micrornas (mirnas) play crucial role ...201727909970
effect of kelp waste extracts on the growth and development of pakchoi (brassica chinensis l.).to explore the effects of kelp waste extracts (kwe) on the growth and development of brassia chinensis l., germination and greenhouse experiments were carried out under different concentrations of kwe. the results showed that a higher germination percentage (95%), associated with high germination index (8.70), germination energy (71.67%) and seedling vigor index (734.67), was obtained under a lower kwe concentration (2%) compared with the control. the radicle length (4.97 cm), fresh weight (0.32 ...201627934911
hexavalent chromium stress enhances the uptake of nitrate but reduces the uptake of ammonium and glycine in pak choi (brassica chinensis l.).chromium (cr) pollution affects plant growth and biochemical processes, so, the relative uptake of glycine, nitrate, and ammonium by pak choi (brassica chinensis) seedlings in treatments with 0mgl-1 and 10mgl-1 cr (vi) were detected by substrate-specific 15n-labelling in a sterile environment. the short-term uptake of 15n-labelled sources and 15n-enriched amino acids were detected by gas chromatography mass spectrometry to explore the mechanism by which cr stress affects glycine uptake and metab ...201728189780
effects of glucose on the uptake and metabolism of glycine in pakchoi (brassica chinensis l.) exposed to various nitrogen sources.plants can absorb amino acids as a nitrogen (n) source, and glucose is an important part of root rhizodeposition and the soil sugar pool, which participates in the regulation of plant growth and uptake. in pakchoi, the effect of glucose concentration on the glycine n uptake from a nutrient mixture composed of glycine, ammonium, and nitrate, or from a single n solution of glycine alone was studied using specific substrate (15)n-labeling and (15)n-gas chromatography mass spectrometry.028253854
transcriptomic profiling of two pak choi varieties with contrasting anthocyanin contents provides an insight into structural and regulatory genes in anthocyanin biosynthetic pathway.the accumulation of anthocyanin in horticultural crops not only improves their stress tolerances but also their nutritional values. many key regulatory and structural genes in anthocyanin biosynthesis have been identified in model plants, but limited information is available for non-model plant species featured with colored leaves. in this study, two pak choi varieties with green or purple leaves were selected to analyze the anthocyanin biosynthesis through rna-seq.201728399809
composite of pah-degrading endophytic bacteria reduces contamination and health risks caused by pahs in vegetables.vegetables accumulate polycyclic aromatic hydrocarbons (pahs) at high concentrations when grown in contaminated sites. inoculation with pah-degrading endophytic bacteria (ebpah) has been recognized as one of the most promising ways to remove pahs from plant bodies; however, the performance of single endophytic bacteria is generally limited. this investigation used a composite of eight ebpah to reduce the contamination and health risk posed by 16 epa priority pahs in vegetables including chinese ...028448936
elevation of secondary metabolites synthesis in brassica campestris ssp. chinensis l. via exogenous inoculation of piriformospora indica with appropriate fertilizer.this work evaluated the impact of exogenous soil inoculation of beneficial fungal strain piriformospora indica on phytochemical changes and the related genes expression of chinese cabbage (brassica campestris ssp. chinensis l.) by greenhouse pot experiments. high performance liquid chromatography (hplc) affirmed that among the different combinations of fungal and organic fertilizer treatments, the phenolic acids and flavonoids were considerably enriched in organic fertilizer and fungi (op) follo ...201728493970
effect of calcium bentonite on zn and cu mobility and their accumulation in vegetable growth in soil amended with compost during consecutive planting.in this study, greenhouse pot experiment was carried out to investigate the cu and zn mobility and their accumulations in pakchoi (brassica chinensis l.) and chinese cabbage (brassica rapachinensis l.) growth in ca-bentonite-pig manure co-compost (cbc)-soil system during three consecutive planting. five composts (0, 2.5, 5, 7.5, and 10% ca-bentonite-pig manure co-composts) amended soil with 5% (w/w) application rate and compared to control without any additive. the results showed that the additi ...201728523619
pulsed light-emitting diodes for a higher phytochemical level in microgreens.a novel research of pulsed light-emitting diode (led) lighting versus continuous lighting was conducted by analyzing phytochemical levels in microgreens. red pak choi (brassica rapa var. chinensis), mustard (brassica juncea l.), and tatsoi (brassica rapa var. rosularis) were grown indoors under hps lamps supplemented with monochromatic (455, 470, 505, 590, and 627 nm) leds [total photosynthetic photon flux density (ppfd) of 200 ± 10 μmol m-2 s-1, for 16 h day-1]. for pulsed light treatments, the ...201728618783
field crops (ipomoea aquatica forsk. and brassica chinensis l.) for phytoremediation of cadmium and nitrate co-contaminated soils via rotation with sedum alfredii hance.phytoremediation coupled with crop rotation (pcc) is a feasible strategy for remediation of contaminated soil without interrupting crop production. the objective of this study was to develop a pcc technology system for greenhouse fields co-contaminated with cd and nitrate using hyperaccumulator sedum alfredii. in this system, endophytic bacterium m002 inoculation, co2 fertilization, and fermentation residue were continuously applied to improve the growth of s. alfredii, and low-accumulator ipomo ...201728669090
improvement of pig manure compost lignocellulose degradation, organic matter humification and compost quality with medical stone.the present study aimed to investigate the effect of different concentrations (0%, 2.5%, 5.0%, 7.5% and 10.0%) of medical stone (ms) on the lignocellulose degradation and organic matter humification during pig manure (pm) composting. the results indicated that the addition of ms drastically promoted the organic carbon and lignin degradation. compared to the control, the decomposition rate of hemicellulose and cellulose was increased by 9.64-27.08% and 2.11-12.07% in ms added treatments. meanwhil ...201728711806
abscisic acid treatment alleviates cadmium toxicity in purple flowering stalk (brassica campestris l. ssp. chinensis var. purpurea hort.) seedlings.the aim of this research was to investigate how exogenous abscisic acid (aba) alleviates cadmium (cd) toxicity in purple flowering stalk (brassica campestris l. ssp. chinensis) and evaluate whether it could be a potential choice for phytoremediation. purple flowering stalk seedlings were cultivated in a hydroponic system with cd at various concentrations (0-100 μmol l-1) as controls and cd plus aba as the treatment in the growth media. the soluble proteins, chlorophyll contents and the activity ...201728755629
isolation and characterization of a phosphorus-solubilizing bacterium from rhizosphere soils and its colonization of chinese cabbage (brassica campestris ssp. chinensis).phosphate-solubilizing bacteria (psb) can promote the dissolution of insoluble phosphorus (p) in soil, enhancing the availability of soluble p. thus, their application can reduce the consumption of fertilizer and aid in sustainable agricultural development. from the rhizosphere of chinese cabbage plants grown in yangling, we isolated a strain of psb (yl6) with a strong ability to dissolve p and showed that this strain promoted the growth of these plants under field conditions. however, systemati ...201728798725
the influence of particle size and feedstock of biochar on the accumulation of cd, zn, pb, and as by brassica chinensis l.biochar produced from rice straw (rc) and maize stalk (mc) was amended to the heavy metal-contaminated soil to investigate the effects of different biochar feedstock and particle size (fine, moderate, coarse) on the accumulation of cd, zn, pb, and as in brassica chinensis l. (chinese cabbage). the concentrations of cd, zn, and pb in shoot were decreased by up to 57, 75, and 63%, respectively, after biochar addition (4%). only mc decreased as concentration in b. chinensis l. shoots by up to 61%. ...201728801768
bioaccessibility and human exposure assessment of cadmium and arsenic in pakchoi genotypes grown in co-contaminated soils.in many countries cadmium (cd) and arsenic (as) commonly coexist in soils contaminated by mining activities, and can easily enter the human body via consumption of leafy vegetables, like the popularly consumed pakchoi (brassica chinensis l.), causing major health concerns. in the present study, bioaccessibility and human exposure of cd and as were assessed in twenty genotypes of pakchoi cultured at two different levels of co-contamination to identify low health risk genotypes. the bioaccessibili ...201728850097
development of est-ssr markers in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) based on de novo transcriptomic assemblies.flowering chinese cabbage is one of the most important vegetable crops in southern china. genetic improvement of various agronomic traits in this crop is underway to meet high market demand in the region, but the progress is hampered by limited number of molecular markers available in this crop. this study aimed to develop est-ssr markers from transcriptome sequences generated by next-generation sequencing. rna-seq of eight cabbage samples identified 48,975 unigenes. of these unigenes, 23,267 we ...201728902884
sulfur protects pakchoi (brassica chinensis l.) seedlings against cadmium stress by regulating ascorbate-glutathione metabolism.cadmium (cd) pollution in food chains pose a potential health risk for humans. sulfur (s) is a significant macronutrient that plays a significant role in the regulation of plant responses to diverse biotic and abiotic stresses. however, no information is currently available about the impact of s application on ascorbate-glutathione metabolism (asa-gsh cycle) of pakchoi plants under cd stress. the two previously identified genotypes, namely, aikangqing (a cd-tolerant cultivar) and qibaoqing (a cd ...201728933771
comparing the influence of selenite (se4+) and selenate (se6+) on the inhibition of the mercury (hg) phytotoxicity to pak choi.selenite (se (iv)) and selenate (se (iv)) have recently been demonstrated to be equally effective in inhibiting mercury (hg) phytotoxicity to plants. this assertion is still unclear. in this study, we aimed to explore the potential effects of se species (se4+ and se6+) on the inhibition of the mercury (hg) bioavailability to pak choi in dry land. pot experiments with exposure to different dosages of mercuric chloride (hgcl2) and selenite (na2seo3) or selenate (na2seo4) were treated. to compare t ...201828968942
effects of split applications of nitrogen fertilizers on the cd level and nutritional quality of chinese cabbage.cadmium (cd) contamination in soil is an increasingly serious problem. management of plant nutrients has been proposed as a potentially promising strategy for minimizing cd accumulation in crops grown in contaminated soil. this study investigated the effects of split applications of nitrogen (n) fertilizers on the cd concentration in chinese cabbage (brassica chinensis l.) plants grown in cd-contaminated soil. compared with single applications, split applications of ammonium or urea resulted in ...201728990380
comparison of dgt with traditional extraction methods for assessing arsenic bioavailability to brassica chinensis in different soils.several predictive models and methods have been used for heavy metals bioavailability, but there is no universally accepted approach in evaluating the bioavailability of arsenic (as) in soil. the technique of diffusive gradients in thin-films (dgt) is a promising tool, but there is a considerable debate with respect to its suitability. the dgt method was compared with other traditional chemical extractions techniques (soil solution, nahco3, nh4cl, hcl, and total as method) for estimating as bioa ...201829032263
comparison of dgt with traditional methods for assessing cadmium bioavailability to brassica chinensis in different soils.there is no universally accepted method for evaluating cadmium (cd) bioavailability in soil. the diffusive gradient in thin films (dgt) technique is a promising tool, but there is considerable debate about its suitability. the ability of this technique to estimate cd bioavailability in soils was compared with the abilities of other traditional chemical extraction techniques (soil solution, ethylene diamine tetraacetic acid (edta), acetic acid (hac), calcium chloride (cacl2), and pseudo-total cd ...201729079727
elevated co2 levels enhance the uptake and metabolism of organic nitrogen.the effects of elevated co2 (eco2 ) on the relative uptake of inorganic and organic nitrogen (n) are unclear. the uptake of different n sources by pak choi (brassica chinensis l.) seedlings supplied with a mixture of nitrate, glycine and ammonium was studied using 15 n-labelling under ambient co2 (aco2 ) (350 ppm) or eco2 (650 ppm) conditions. 15 n-labelled short-term uptake and 15 n-gas chromatography mass spectrometry (gc-ms) were applied to measure the effects of eco2 on glycine uptake and me ...201729080266
plant growth, antibiotic uptake, and prevalence of antibiotic resistance in an endophytic system of pakchoi under antibiotic exposure.antibiotic contamination in agroecosystems may cause serious problems, such as the proliferation of various antibiotic resistant bacteria and the spreading of antibiotic resistance genes (args) in the environment or even to human beings. however, it is unclear whether environmental antibiotics, antibiotic resistant bacteria, and args can directly enter into, or occur in, the endophytic systems of plants exposed to pollutants. in this study, a hydroponic experiment exposing pakchoi (brassica chin ...201729099753
dynamics of multiple elements in fast decomposing vegetable residues.litter decomposition regulates the cycling of nutrients and toxicants but is poorly studied in farmlands. to understand the unavoidable in-situ decomposition process, we quantified the dynamics of c, h, n, as, ca, cd, cr, cu, fe, hg, k, mg, mn, na, ni, pb, and zn during a 180-d decomposition study in leafy lettuce (lactuca sativa var. longifoliaf) and rape (brassica chinensis) residues in a wastewater-irrigated farmland in northwestern china. different from most studied natural ecosystems, the m ...201829100695
effects of biochar on phytotoxicity and translocation of polybrominated diphenyl ethers in ni/fe bimetallic nanoparticle-treated soil.in this study, soil culture experiments were conducted to explore the effects of biochar-supported ni/fe nanoparticles on the accumulation and translocation of polybrominated diphenyl ethers (pbdes) in soil-plant system and its phytotoxicity to brassica chinensis. compared with those in bde209 contaminated soils (s 1) and ni/fe nanoparticle-treated soil (s 3), the plant biomass, root, and shoot lengths in biochar-supported ni/fe nanoparticle-treated soil (s 4) were increased by 23 mg, 1.35 cm, a ...201829128943
subcellular distribution and biotransformation of phenanthrene in pakchoi after inoculation with endophytic pseudomonas sp. as probed using hrms coupled with isotope-labeling.contamination of food-crops with polycyclic aromatic hydrocarbons (pahs) poses a grave concern to food safety, especially when pahs are internalized. we have demonstrated in our previous study that inoculation of crop with a phenanthrene-degrading endophytic pseudomonas sp. ph6-gfp could overcome this problem. here, the subcellular distribution and biotransformation mechanism of phenanthrene in pakchoi (brassica chinensis l.) seedlings with inoculation of ph6-gfp were further investigated both i ...201729150254
identification and fine mapping of a stay-green gene (brnye1) in pakchoi (brassica campestris l. ssp. chinensis).using bulked segregant analysis combined with next-generation sequencing, we delimited the brnye1 gene responsible for the stay-green trait of nye in pakchoi. sequence analysis identified bra019346 as the candidate gene. "stay-green" refers to a plant trait whereby leaves remain green during senescence. this trait is useful in the cultivation of pakchoi (brassica campestris l. ssp. chinensis), which is marketed as a green leaf product. this study aimed to identify the gene responsible for the st ...201729209732
transcriptomic analysis of pak choi under acute ozone exposure revealed regulatory mechanism against ozone stress.ground-level ozone (o3) is one of the major air pollutants, which cause oxidative injury to plants. the physiological and biochemical mechanisms underlying the responses of plants to o3 stress have been well investigated. however, there are limited reports about the molecular basis of plant responses to o3. in this study, a comparative transcriptomic analysis of pak choi (brassica campestris ssp. chinensis) exposed to different o3 concentrations was conducted for the first time.201729216819
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