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synthesis and bioactivity study of 2-acylamino-substituted n'-benzylbenzohydrazide derivatives.the discovery of new safe and effective pesticides is one of the main means of providing eco-friendly agricultural agents for modern crop protection. to identify new biological molecules based of the anthranilic diamide skeleton of the novel pesticide chlorantraniliprole, which acts on the ryanodine receptor and functional groups in acyl hydrazine insect growth regulators, more than 40 new compounds of 2-acylamino-substituted n'-benzylbenzohydrazide derivatives were designed and synthesized. the ...201223088717
development of transgenic cryia(c) + gna cotton plants via pollen tube pathway method confers resistance to helicoverpa armigera and aphis gossypii glover.elite cotton cultivar sumian16 was transformed with p7rpsbk-mgna-nptii containing bt (cryia(c)), galanthus nivalis agglutinin (gna) resistance genes and selectable marker nptii gene via the pollen tube pathway method and two fertile transgenic bt + gna plants were obtained in the present study. the integration and expression of the bt and gna genes were confirmed by molecular biology techniques and insect bioassays. insect bioassays showed that the transformed plants were highly toxic to bollwor ...201323143495
expression of small rna in aphis gossypii and its potential role in the resistance interaction with melon.the regulatory role of small rnas (srnas) in various biological processes is an active area of investigation; however, there has been limited information available on the role of srnas in plant-insect interactions. this study was designed to identify srnas in cotton-melon aphid (aphis gossypii) during the vat-mediated resistance interaction with melon (cucumis melo).201223173035
spatio-temporal dynamics of viruses are differentially affected by parasitoids depending on the mode of transmission.relationships between agents in multitrophic systems are complex and very specific. insect-transmitted plant viruses are completely dependent on the behaviour and distribution patterns of their vectors. the presence of natural enemies may directly affect aphid behaviour and spread of plant viruses, as the escape response of aphids might cause a potential risk for virus dispersal. the spatio-temporal dynamics of cucumber mosaic virus (cmv) and cucurbit aphid-borne yellows virus (cabyv), transmitt ...201223202516
evidence of superclones in australian cotton aphid aphis gossypii glover (aphididae: hemiptera).aphis gossypii is an important pest of cotton that has developed resistance to many chemicals used for its control. any lack of understanding of its genetic structure, resistance status and host plant specialisation hampers effective management.201323292942
outcomes of co-infection by two potyviruses: implications for the evolution of manipulative strategies.recent studies have documented effects of plant viruses on host plants that appear to enhance transmission by insect vectors. but, almost no empirical work has explored the implications of such apparent manipulation for interactions among co-infecting pathogens. we examined single and mixed infections of two potyviruses, watermelon mosaic virus (wmv) and zucchini yellow mosaic virus (zymv), that frequently co-occur in cucurbitaceae populations and share the same aphid vectors. we found that zymv ...201323407835
pest trade-offs in technology: reduced damage by caterpillars in bt cotton benefits aphids.the rapid adoption of genetically engineered (ge) plants that express insecticidal cry proteins derived from bacillus thuringiensis (bt) has raised concerns about their potential impact on non-target organisms. this includes the possibility that non-target herbivores develop into pests. although studies have now reported increased populations of non-target herbivores in bt cotton, the underlying mechanisms are not fully understood. we propose that lack of herbivore-induced secondary metabolites ...201323486438
comparative transcriptome analysis of gossypium hirsutum l. in response to sap sucking insects: aphid and whitefly.cotton (gossypium hirsutum l.) is a major fiber crop that is grown worldwide; it faces extensive damage from sap-sucking insects, including aphids and whiteflies. genome-wide transcriptome analysis was performed to understand the molecular details of interaction between gossypium hirsutum l. and sap-sucking pests, namely aphis gossypii (aphid) and bemisia tabacci (whiteflies). roche's gs-titanium was used to sequence transcriptomes of cotton infested with aphids and whiteflies for 2 h and 24 h.201323577705
special plant species determines diet breadth of phytophagous insects: a study on host plant expansion of the host-specialized aphis gossypii glover.host specialization is a ubiquitous character of phytophagous insects. the polyphagous population is usually composed of some subpopulations that can use only a few closely related plants. cotton-melon aphids, aphis gossypii glover exhibited strong host specialization, and the cotton- and cucurbits-specialized biotypes had been clearly identified. however, the experimental work that addressed the roles of plant species in determining diet breadth of phytophagous insects is rare. in the present s ...201323580128
agroinoculation of a full-length cdna clone of cotton leafroll dwarf virus (clrdv) results in systemic infection in cotton and the model plant nicotiana benthamiana.cotton blue disease is the most important viral disease of cotton in the southern part of south america. its etiological agent, cotton leafroll dwarf virus (clrdv), is specifically transmitted to host plants by the aphid vector (aphis gossypii) and any attempt to perform mechanical inoculations of this virus into its host has failed. this limitation has held back the study of this virus and the disease it causes. in this study, a full-length cdna of clrdv was constructed and expressed in vivo un ...201323623981
formation of stylet sheaths in āere (in air) from eight species of phytophagous hemipterans from six families (suborders: auchenorrhyncha and sternorrhyncha).stylet sheath formation is a common feature among phytophagous hemipterans. these sheaths are considered essential to promote a successful feeding event. stylet sheath compositions are largely unknown and their mode of solidification remains to be elucidated. this report demonstrates the formation and solidification of in āere (in air) produced stylet sheaths by six hemipteran families: diaphorina citri (psyllidae, asian citrus psyllid), aphis nerii (aphididae, oleander/milkweed aphid), toxopter ...201323638086
synergistic manipulations of plant and insect defences.it has been demonstrated previously that cis-jasmone acts as an elicitor of plant defence mechanism(s) by inducing secondary metabolism. it has also been demonstrated that temporal synergism can result in hypersensitive insect pests due to the inhibition of metabolic enzymes.201423653417
genetic diversity and potential vectors and reservoirs of cucurbit aphid-borne yellows virus in southeastern spain.the genetic variability of a cucurbit aphid-borne yellows virus (cabyv) (genus polerovirus, family luteoviridae) population was evaluated by determining the nucleotide sequences of two genomic regions of cabyv isolates collected in open-field melon and squash crops during three consecutive years in murcia (southeastern spain). a phylogenetic analysis showed the existence of two major clades. the sequences did not cluster according to host, year, or locality of collection, and nucleotide similari ...201323802870
cotton aphid (heteroptera: aphididae) susceptibility to commercial and experimental insecticides in the southern united states.cotton aphid, aphis gossypii glover, has a history of developing resistance to novel insecticides. a program is needed to monitor cotton aphid susceptibility to new insecticides. concentration-mortality bioassays were conducted from 2008 to 2011 to monitor the susceptibility of cotton aphids from fields across the midsouthern united states to thiamethoxam and sulfoxaflor. flonicamid was included in 2010 and 2011. bioassays followed the procedures described by the insecticide resistance action co ...201323865211
biology and potentiality in biological control of micraspis discolor (fabricius) (coleoptera: coccinellidae).hosts influence on growth duration and abundance of coccinellid predators. micraspis discolor (fabricius) (coleoptera: coccinellidae) has been well known as both carnivorous and pollinivorous. work was carried out as a series of laboratory-based influence of host species aphis gossypii (clover) (hemiptera: aphididae) and green rice leafhopper, nephotettix sp. (hemiptera: cicadellidae) on m. discolors development and effect of rice growth stage on m. discolor adult preference. for field study, po ...201223885421
host races of the cotton aphid, aphis gossypii, in asexual populations from wild plants of taro and brinjal.worldwide, several studies have shown that adaptation to different host plants in phytophagous insects can promote speciation. the cotton aphid, aphis gossypii glover (homoptera: aphididae: aphidini), is a highly polyphagous species, but its populations increase by parthenogenetic reproduction alone in indian subcontinent. this study showed that genotypes living in wild plants of taro, colocasia esculenta var. esculenta (l.) schott (alismatales: araceae), and brinjal, solanum torvum swartz (sola ...201323895554
responses of an aphidophagous ladybird beetle, anegleis cardoni, to varying densities of aphis gossypii.laboratory experiments were conducted to determine the functional and numerical responses of fourth instar larvae, adult male, and adult female ladybird beetles, anegleis cardoni weise (coleoptera: coccinellidae), to different densities of aphids, aphis gossypii glover (hemiptera: aphididae), on the bottle gourd, lagenaria vulgaris seringe (cucurbitales: cucurbitaceae). the results revealed a density dependent, type ii functional response of a. cardoni. prey consumption increased curvilinearly w ...201323901912
field evaluation of bt cotton crop impact on nontarget pests: cotton aphid and boll weevil.bt cotton plants expressing cry1ac protein have high specificity for the control of lepidopteran larvae. however, studies conducted in several countries have shown these plants have a differential impact on nontarget herbivores. the aim of this study was to compare the colonization rates and population abundance of the cotton aphid, aphis gossypii glover (hemiptera: aphididae) and the boll weevil, anthonomus grandis boheman (coleoptera: curculionidae), in plots of bt (nuopal) and non-bt cotton ( ...201323949719
potential cotton aphid, aphis gossypii, population suppression by arthropod predators in upland cotton.the cotton aphid, aphis gossypii glover, predation rate of convergent lady beetle, hippodamia convergens guerin-meneville, was determined by assigning a single predator randomly to each of four prey density treatments in the laboratory. prey densities included 25, 50, 100, and 200 aphids per petri dish arena. predation response was recorded at 1, 4, 8, 16, 24, and 48 h after assigning predators to their prey treatments. rate of consumption increased through time, with all 25 aphids consumed duri ...201323956125
seasonal phenology and species composition of the aphid fauna in a northern crop production area.the species diversity of aphids and seasonal timing of their flight activity can have significant impacts on crop production, as aphid species differ in their ability to transmit plant viruses and flight timing affects virus epidemiology. the aim of the study was to characterise the species composition and phenology of aphid fauna in finland in one of the northernmost intensive crop production areas of the world (latitude 64°).201323967149
[effects of temperature and density on the mortality and reproduction of cotton aphid aphis gossypii].a laboratory cotton leaf disc experiment was conducted to study the effects of different temperature (32, 34, 36, 38, and 40 degrees c) and density (5, 25, 50, and 75 individuals per dish) on the mortality and reproduction of aphis gossypii. with the increase of temperature, density, and culture duration, the cumulative mortality of a. gossypii presented an increasing trend. the parameters estimated by complementary log-log (cll) model showed that the beta value decreased with the increase of de ...201324015547
[effects of host plants on the life table parameters of experimental populations of aphis gossypii].a comparative study was conducted on the life table parameters of aphis gossypii reared on five host plant species at (25 +/- 1) degrees c in laboratory. there existed significant differences in the durations of various developmental stages, adult longevity, mean offspring number per day, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time, and population doubling time for the a. gossypii populations reared on the host plants. for the aphids on lagena ...201324015567
population dynamics of three aphid species (hemiptera: aphididae) on four ipomoea spp. infected or noninfected with sweetpotato potyviruses.three aphid species, aphis gossypii glover and myzus persicae (sulzer) (efficient sweetpotato potyvirus vectors) and rhopalosiphum padi (l.) (an inefficient vector), are commonly found in sweet potato, ipomoea batatas (l.), in louisiana. field-grown sweet potatoes are naturally infected with several potyviruses: sweet potato feathery mottle virus, sweet potato virus g, and sweet potato virus 2. thus, these aphids commonly encounter virus-infected hosts. what is not known is how each of these aph ...201324020267
monitoring of aphid flight activities in seed potato crops in serbia.aphid flight activities in seed potato fields have been studied by the yellow water traps. it is a good method for monitoring aphids as vectors of viruses, but this study also showed it is a suitable method for insect-diversity research. during the four-year studies, over 11.500 specimens were collected and a total of 107 different taxa of aphids were identified. the most abundant species were polyphagous species, such as: acyrthosiphon pisum (haris), aphis fabae scopoli, aphis gossypii gloveran ...201324039529
aphids (hemiptera, aphididae) on ornamental plants in greenhouses in bulgaria.investigations on the species composition and host range of aphids on ornamental greenhouse plants in bulgaria was conducted over a period of five years, from 2008 to 2012. twenty greenhouses, growing ornamentals for landscaping, plant collections and other purposes were observed. they were located in the regions of sofia, plovdiv, smolyan, pavlikeni, varna and burgas. the total number of collected aphid samples was 279. their composition included 33 aphid species and one subspecies from 13 gene ...201324039530
identification and expression profiling of odorant binding proteins and chemosensory proteins between two wingless morphs and a winged morph of the cotton aphid aphis gossypii glover.insects interact with their environment and respond to the changes in host plant conditions using semiochemicals. such ecological interactions are facilitated by the olfactory sensilla and the use of olfactory recognition proteins. the cotton aphid aphis gossypii can change its phenotype in response to ecological conditions. they reproduce mainly as wingless asexual morphs but develop wings to find mates or new plant hosts under the influence of environmental factors such as temperature, plant n ...201324073197
the mitochondrial genome of the russian wheat aphid diuraphis noxia: large repetitive sequences between trne and trnf in aphids.to characterize aphid mitochondrial genome (mitogenome) features, we sequenced the complete mitogenome of the russian wheat aphid, diuraphis noxia. the 15,784-bp mitogenome with a high a+t content (84.76%) and strong c skew (-0.26) was arranged in the same gene order as that of the ancestral insect. unlike typical insect mitogenomes, d. noxia possessed a large tandem repeat region (644 bp) located between trne and trnf. sequencing partial mitogenome of the cotton aphid (aphis gossypii) further c ...201424095774
a genetic map of melon (cucumis melo l.) with rflp, rapd, isozyme, disease resistance and morphological markers.one hundred and ten markers were analysed for linkage in 218 f2 plants derived from two divergent cultivars ('védrantais' and 'songwhan charmi') of cucumis melo (l.). thirty-four rflps, 64 rapds, one isozyme, four disease resistance markers and one morphological marker were used to construct a genetic map spanning 14 linkage groups covering 1390 cm of the melon genome. rapd and rflp markers detected similar polymorphism levels. rflps were largely due to base substitutions rather than insertion/d ...199624162199
insect predators and control of aphis gossypii comparing to certain insecticides under caged-cotton plants conditions.numerous beneficial arthropods prey on aphid populations in cotton fields. field experiments were conducted at kafr el-sheikh governorate, egypt in seasons of 2010 and 2011 to examine the impact of naturally occurring insect predators on cotton aphid, aphis gossypii glover (homoptera: aphididae) comparing to the impact of three insecticides; i.e., diafenthiuron, imidacloprid and thiamethoxam at their recommended rates using caged-plants technique. the toxicity of these insecticides to insect pre ...201324175433
synthesis and biological evaluation of nitromethylene neonicotinoids based on the enhanced conjugation.the neonicotinoids with a nitroconjugated system had excellent bioactivity, which could rival imidacloprid, and has been previously reported. however, the photodegradation and hydrolysis of this series of neonicotinoids was very quick according to our further investigation, which cannot be developed as a pesticide further. the approach to further enhance the conjugation was tried not only to increase the bioactivities but also to improve the stability in water and in the sun. a substituted pheny ...201324180522
plant virus-induced changes in aphid population development and temporal fluctuations in plant nutrients.cucurbita pepo plants were infected with zucchini yellow mosaic virus or maintained noninfected.aphis gossypii, which transmits the virus, lived longer and produced more offspring on infected than on noninfected plants. on infected plants, the intrinsic rate of natural increase fora. gossypii increased with time after inoculation. in a similar experiment, concentrations of phloem sap nutrients, including free amino acids, total protein, and sugars from infected and noninfected plants were compar ...199424242121
plant resistance to aphid feeding: behavioral, physiological, genetic and molecular cues regulate aphid host selection and feeding.aphids damage major world food and fiber crops through direct feeding and transmission of plant viruses. fortunately, the development of many aphid-resistant crop plants has provided both ecological and economic benefits to food production. plant characters governing aphid host selection often dictate eventual plant resistance or susceptibility to aphid herbivory, and these phenotypic characters have been successfully used to map aphid resistance genes. aphid resistance is often inherited as a d ...201424282145
a non-persistently transmitted-virus induces a pull-push strategy in its aphid vector to optimize transmission and spread.plant viruses are known to modify the behaviour of their insect vectors, both directly and indirectly, generally adapting to each type of virus-vector relationship in a way that enhances transmission efficiency. here, we report results of three different studies showing how a virus transmitted in a non-persistent (np) manner (cucumber mosaic virus; cmv, cucumovirus) can induce changes in its host plant, cucumber (cucumis sativus cv. marumba) that modifies the behaviour of its aphid vector (aphis ...201424373951
ecological adaption analysis of the cotton aphid (aphis gossypii) in different phenotypes by transcriptome comparison.the cotton aphid, aphis gossypii glover, is a destructive insect pest worldwide; it directly or indirectly damages (virus transmission) 300 species of host plants. knowledge of their ecologically adaptive mechanisms at the molecular level may provide an essential and urgent method to effectively control this pest. however, no transcriptome information is available for the cotton aphid and sequence data are scarce. therefore, we obtained transcriptome data.201324376660
identification of two acetylcholinesterases in pardosa pseudoannulata and the sensitivity to insecticides.pardosa pseudoannulata is an important predatory enemy against insect pests, such as rice planthoppers and leafhoppers. in order to understand the insecticide selectivity between p. pseudoannulata and insect pests, two acetylcholinesterase genes, pp-ace1 and pp-ace2, were cloned from this natural enemy. the putative proteins encoded by pp-ace1 and pp-ace2 showed high similarities to insect ache1 (63% to liposcelis entomophila ache1) and ache2 (36% to culex quinquefasciatus ache2) with specific f ...201424463359
cotton photosynthesis-related psak1 protein is involved in plant response to aphid attack.it is believed that hundreds of genes, including photosynthesis-related genes, are typically involved in plant response to aphid feeding. up to now, however, it is little known on the relationship between the photosynthesis-related genes and plant response to herbivores. in this study, we identified a cotton photosynthesis-related gene (ghpsak1) which belongs to psi-psak family and encodes a putative protein of 162 amino acids. rt-pcr analysis revealed that ghpsak1 transcripts in leaves were inc ...201424469731
indirect multi-trophic interactions mediated by induced plant resistance: impact of caterpillar feeding on aphid parasitoids.cotton produces insecticidal terpenoids that are induced by tissue-feeding herbivores. damage by heliothis virescens caterpillars increases the terpenoid content, which reduces the abundance of aphids. this effect is not evident in bt-transgenic cotton, which is resistant to h. virescens. we determined whether induction of terpenoids by caterpillars influences the host quality of aphis gossypii for the parasitoid lysiphlebus testaceipes and whether this interaction is influenced by bt cotton. th ...201424522627
how does the host-specialized aphid deal with food deficiency?aphis gossypii glover shows obvious host specialization, with cucurbit- and cotton-specialized biotypes or host races in many regions. because its annual natal host crops senesce earlier the cucurbit-specialized biotype may suffer food deficiency. the method this biotype uses to overcome this challenge is still poorly understood. in order to understand the potential of the cucurbit-specialized biotype aphids in host shift and usage, the performance of this biotype on cotton (gossypium hirsutum), ...201424526656
a type-iii insect geranylgeranyl diphosphate synthase with a novel catalytic property.geranylgeranyl diphosphate synthase (ggpp synthase or ggpps) is among the short-chain prenyltransferases, catalyzing the formation of the acyclic precursor ggpp for the biosynthesis of a variety of isoprenoids. ggppss have been extensively studied in plants, eubacteria, archaebacteria, yeast and mammals, but up to now information about an insect ggpps is still scarce. here we cloned the cdna encoding an insect ggpps from the cotton aphid (designated as agggpps). agggpps had an open reading frame ...201424527738
spore density and viability of entomopathogenic fungal isolates from indonesia, and their virulence against aphis gossypii glover (homoptera: aphididae).the focus of this study was on quantifying fitness attributes, such as spore density and viability, and determining the virulence level against aphid (aphis gossypii) nymphs of isolates from the fungal species beauveria bassiana and metarhizium anisopliae. the fungal isolates were obtained from several insects, including plutella xylostella, hypothenemus hampei, bronstispa longissima, a. gossypii, tenebrio molitor, and leptocorisa acuta, that were collected from indonesian islands, such as sumat ...201024575186
why the aphid aphis spiraecola is more abundant on clementine tree than aphis gossypii?aphis spiraecola and aphis gossypii cause harmful damages on clementine tree orchards. weekly surveys measured the abundance of aphids (larvae, winged and wingless adults) as well as of auxiliary insects and parameters of energy metabolism. correlatively, soluble carbohydrates, total free amino acids, free proline and condensed tannins were quantified in control and infested leaves. both aphid species showed parallel temporal variations, but a. spiraecola was consistently more abundant regardles ...201424581807
pathogenicity of conidia-based preparations of entomopathogenic fungi against the greenhouse pest aphids myzus persicae, aphis gossypii, and aulacorthum solani (hemiptera: aphididae).seeking new isolates of entomopathogenic fungi with greater virulence against greenhouse aphid pests than those currently registered in north america for control of these insects, single-dose screening assays of 44 selected fungal isolates and 4 commercially available strains were conducted against first-instar nymphs of myzus persicae and aphis gossypii. the assays identified a number of beauveria and metarhizium isolates with virulence equal to or greater than that of the commercial strains ag ...201424583227
quantification of the pirimicarb resistance allele frequency in pooled cotton aphid (aphis gossypii glover) samples by taqman snp genotyping assay.pesticide resistance monitoring is a crucial part to achieving sustainable integrated pest management (ipm) in agricultural production systems. monitoring of resistance in arthropod populations is initially performed by bioassay, a method that detects a phenotypic response to pesticides. molecular diagnostic assays, offering speed and cost improvements, can be developed when the causative mutation for resistance has been identified. however, improvements to throughput are limited as genotyping m ...201424614533
gene expression profiling in winged and wingless cotton aphids, aphis gossypii (hemiptera: aphididae).while trade-offs between flight capability and reproduction is a common phenomenon in wing dimorphic insects, the molecular basis is largely unknown. in this study, we examined the transcriptomic differences between winged and wingless morphs of cotton aphids, aphis gossypii, using a tag-based digital gene expression (dge) approach. ultra high-throughput illumina sequencing generated 5.30 and 5.39 million raw tags, respectively, from winged and wingless a. gossypii dge libraries. we identified 1 ...201424644424
isolation, characterization, and insecticidal activity of an endophyte of drunken horse grass, achnatherum inebrians.abstract endophytic microorganisms reside within plant tissues and have often been found to promote plant growth. in this study, endophytic microorganisms were isolated from the roots, stems, leaves, and seeds of healthy drunken horse grass, achnatherum inebrians (hance) keng (poales: poaceae), through the use of a grinding separation method and identified by a dual approach of morphological and physiological observation and 16s rrna gene-based (for bacteria) and internal transcribed sequence-ba ...201324784492
complete mitochondrial genome of aphis gossypii glover (hemiptera: aphididae).the complete mitochondrial genome of the cotton-melon aphid, aphis gossypii glover, was sequenced using a combination of high-throughput sequencing, traditional pcr amplification, and sanger sequencing. the genome is 15,869 bp in length, and contains 37 typical coding genes, one non-coding at-rich region, and a repeat region found exclusively in aphids. the base composition of the genome is a (45.4%), t (38.3%), c (10.4%), and g (5.9%). all protein coding genes start with a typical atn initiatio ...201624865902
evidence of horizontal transfer of non-autonomous lep1 helitrons facilitated by host-parasite interactions.horizontal transfer (ht) of transposable elements has been recognized to be a major force driving genomic variation and biological innovation of eukaryotic organisms. however, the mechanisms of ht in eukaryotes remain poorly appreciated. the non-autonomous helitron family, lep1, has been found to be widespread in lepidopteran species, and showed little interspecific sequence similarity of acquired sequences at 3' end, which makes lep1 a good candidate for the study of ht. in this study, we descr ...201424874102
comparative transcriptional analysis of asexual and sexual morphs reveals possible mechanisms in reproductive polyphenism of the cotton aphid.aphids, the destructive insect pests in the agriculture, horticulture and forestry, are capable of reproducing asexually and sexually upon environmental change. however, the molecular basis of aphid reproductive mode switch remains an enigma. here we report a comparative analysis of differential gene expression profiling among parthenogenetic females, gynoparae and sexual females of the cotton aphid aphis gossypii, using the rna-seq approach with next-generation sequencing platforms, followed by ...201424915491
molecular characterization of the aphis gossypii olfactory receptor gene families.the cotton aphid, aphis gossypii glover, is a polyphagous pest that inflicts great damage to cotton yields worldwide. antennal olfaction, which is extremely important for insect survival, mediates key behaviors such as host preference, mate choice, and oviposition site selection. in insects, odor detection is mediated by odorant receptors (ors) and ionotropic receptors (irs), which ensure the specificity of the olfactory sensory neuron responses. in this study, our aim is to identify chemosensor ...201424971460
the entomopathogenic fungal endophytes purpureocillium lilacinum (formerly paecilomyces lilacinus) and beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions.the effects of two entomopathogenic fungal endophytes, beauveria bassiana and purpureocillium lilacinum (formerly paecilomyces lilacinus), were assessed on the reproduction of cotton aphid, aphis gossypii glover (homoptera:aphididae), through in planta feeding trials. in replicate greenhouse and field trials, cotton plants (gossypium hirsutum) were inoculated as seed treatments with two concentrations of b. bassiana or p. lilacinum conidia. positive colonization of cotton by the endophytes was c ...201425093505
oral delivery mediated rna interference of a carboxylesterase gene results in reduced resistance to organophosphorus insecticides in the cotton aphid, aphis gossypii glover.rna interference (rnai) is an effective tool to examine the function of individual genes. carboxylesterases (care, ec 3.1.1.1) are known to play significant roles in the metabolism of xenobiotic compounds in many insect species. previous studies in our laboratory found that care expression was up-regulated in aphis gossypii (glover) (hemiptera: aphididae) adults of both omethoate and malathion resistant strains, indicating the potential involvement of care in organophosphorus (op) insecticide re ...201425140535
identification of top-down forces regulating cotton aphid population growth in transgenic bt cotton in central china.the cotton aphid aphis gossypii glover is the main aphid pest in cotton fields in the yangtze river valley cotton-planting zone (yrz) in central china. various natural enemies may attack the cotton aphid in bt cotton fields but no studies have identified potential specific top-down forces that could help manage this pest in the yrz in china. in order to identify possibilities for managing the cotton aphid, we monitored cotton aphid population dynamics and identified the effect of natural enemies ...201425170907
the effect of chrysoperla carnea (neuroptera: chrysopidae) and adalia bipunctata (coleoptera: coccinellidae) on the spread of cucumber mosaic virus (cmv) by aphis gossypii (hemiptera: aphididae).the effects of two aphidophagous predators, the larvae of chrysoperla carnea and adults of adalia bipunctata, on the spread of cucumber mosaic virus (cmv) transmitted in a non-persistent manner by the cotton aphid aphis gossypii were studied under semi-field conditions. natural enemies and aphids were released inside insect-proof cages (1 m × 1 m × 1 m) with a central cmv-infected cucumber plant surrounded by 48 healthy cucumber seedlings, and the spatiotemporal dynamics of the virus and vector ...201525208589
aphid honeydew quality as a food source for parasitoids is maintained in bt cotton.bt-transgenic cotton has proven to be highly efficient in controlling key lepidopteran pests. one concern with the deployment of bt cotton varieties is the potential proliferation of non-target pests. we previously showed that bt cotton contained lower concentrations of insecticidal terpenoids as a result of reduced caterpillar damage, which benefited the aphid aphis gossypii. it is thus important that non-target herbivores are under biological control in bt cotton fields. the induction or lack ...201425226521
the vat locus encodes for a cc-nbs-lrr protein that confers resistance to aphis gossypii infestation and a. gossypii-mediated virus resistance.aphis gossypii is a polyphagous sucking aphid and a vector for many viruses. in cucumis melo, a dominant locus, vat, confers a high level of resistance to aphis gossypii infestation and to viruses transmitted by this vector. to investigate the mechanism underlying this double resistance, we first genetically dissected the vat locus. we delimited the double resistance to a single gene that encodes for a coiled-coil-nucleotide-binding-site-leucine-rich repeat (cc-nbs-lrr) protein type. to validate ...201425283874
control of insect vectors and plant viruses in protected crops by novel pyrethroid-treated nets.long-lasting insecticide-treated nets (llitns) constitute a novel alternative that combines physical and chemical tactics to prevent insect access and the spread of insect-transmitted plant viruses in protected enclosures. this approach is based on a slow-release insecticide-treated net with large hole sizes that allow improved ventilation of greenhouses. the efficacy of a wide range of llitns was tested under laboratory conditions against myzus persicae, aphis gossypii and bemisia tabaci. two n ...201525404196
a cryptic species of aphis gossypii (hemiptera: aphididae) complex revealed by genetic divergence and different host plant association.three cryptic species, aphis gossypii, aphis glycines, and aphis rhamnicola sp. nov., are recognized as sharing buckthorn plant, rhamnus spp. as primary hosts. these aphid species have morphological similarities; however, there are significant genetic differences between the three cryptic species. based on the high level of genetic divergence and the different secondary host association, we described a new species, aphis rhamnicola sp. nov., for apterous and alate vivipara, fundatrix, ovipara, a ...201525413997
molecular screening of insecticides with sigma glutathione s-transferases (gst) in cotton aphid aphis gossypii using docking.glutathione s-transferases (gsts) are one of the major families of detoxifying enzymes that detoxifies different chemical compounds including insecticides in different insect species. among the gst subclasses, sigma gsts are found to be the most abundant and conserved among different insect orders. these gsts are found to play an important role in lipid peroxidation as well as detoxification. cotton aphid, aphis gossypii is the most damaging sucking pest with a wide range of hosts and vector of ...201425512683
transcriptomic comparison of thiamethoxam-resistance adaptation in resistant and susceptible strains of aphis gossypii glover.a thiamethoxam-resistant strain of cotton aphid (thr) strain displayed a 19.35-fold greater resistance to thiamethoxam compared to a susceptible cotton aphid (ss) strain. solexa sequencing technology was used to investigate differentially expressed genes (degs) in cotton aphids in the context of thiamethoxam resistance. a total of 22,569,311 and 21,317,732 clean reads were obtained from the thr and ss transcriptomes, respectively, and assembled into 35,222 non-redundant (nr) consensus sequences. ...201525528611
molecular and morphological differentiation between aphis gossypii glover (hemiptera, aphididae) and related species, with particular reference to the north american midwest.the cotton aphid, aphis gossypii, is one of the most biologically diverse species of aphids; a polyphagous species in a family where most are host specialists. it is economically important and belongs to a group of closely related species that has challenged aphid taxonomy. the research presented here seeks to clarify the taxonomic relationships and status of species within the aphid gossypii group in the north american midwest. sequences of the mitochondrial cytochrome oxidase 1 (coi), nuclear ...201425561853
effects of lysiphlebia japonica (ashmead) on cotton-melon aphid aphis gossypii glover lipid synthesis.the cotton-melon aphid, aphis gossypii glover, is a major insect pest worldwide. the wasp lysiphlebia japonica (ashmead) is the predominant parasitoid of cotton-melon aphids in north china. parasitization has been reported to affect host lipids in several systems, but the lipid synthesis-related genes and transcription changes in the cotton-melon aphid-parasitoid interaction are not clear. in this study, 36 lipid synthesis-related genes were cloned and their transcription changes in parasitized ...201525702953
proteomics-based identification and analysis proteins associated with spirotetramat tolerance in aphis gossypii glover.spirotetramat were now widely used for control insecticides resistant aphids since 2011 in china. in order to elucidate the possible resistance mechanism, a laboratory selected resistant strain (sr) of cotton aphid was established with a 578.93-fold and 14.91-fold resistance ratio to spirotetramat for adult aphids and nymph, respectively, as compared with the susceptible strain (ss). in this study, a comparative proteomic analysis between sr and ss strains were conducted aims to better understan ...201525868820
spatial and temporal spread of citrus tristeza virus and its aphid vectors in the north western area of morocco.first report of citrus tristeza virus (ctv,closterovirus) in morocco datesback to 1961 in collections of citrus varieties. an exhaustive survey of citrus in the north of the country in 2009 revealed that ctv was spread all over the citrus production area. we attempted to evaluate the relative contribution of different aphid species in the spread of ctv disease in a citrus reticulata orchard at the loukkous region during 2 years (2012 and 2013). the overall ctv incidence estimated in the experime ...201625884375
inhibition of α-glucosidase activity by n-deoxynojirimycin analogs in several insect phloem sap feeders.secondary metabolites and synthetic iminosugars that structurally resemble monosaccharides are potent inhibitors of α-glucosidase activity. the enzyme is core in cleaving sucrose in phloem feeding insects and it also plays a crucial role of reducing osmotic stress via the formation of oligosaccharides. inhibition of hydrolysis by iminosugars should result in nutritional deficiencies and/or disruption of normal osmoregulation. deoxynojirimycin (dnj) and 2 n-alkylated analogs [n-butyl dnj (nb-dnj) ...201625900765
biocompatible colloidal dispersions as potential formulations of natural pyrethrins: a structural and efficacy study.biocompatible colloidal dispersions of the micro- and nanoemulsion type based on lemon oil terpenes, polysorbates, water, and glycerol were used for the formulation of pyrethrins, botanical insecticides derived from the white pyrethrum daisy, tanacetum cinerariifolium. the proposed formulation is based on pyrethrin-containing water-in-oil (w/o) microemulsions that could be diluted in one step with an aqueous phase to obtain kinetically stable oil-in-water (o/w) nanoemulsions. structural characte ...201525946579
identification of differentially expressed genes related to aphid resistance in cucumber (cucumis sativus l.).cucumber, a very important vegetable crop worldwide, is easily damaged by pests. aphids (aphis gossypii glover) are among the most serious pests in cucumber production and often cause severe loss of yield and make fruit quality get worse. identifying genes that render cucumbers resistant to aphid-induced damage and breeding aphid-resistant cucumber varieties have become the most promising control strategies. in this study, a illumina genome analyzer platform was applied to monitor changes in gen ...201525959296
carboxylesterase-mediated insecticide resistance: quantitative increase induces broader metabolic resistance than qualitative change.carboxylesterases are mainly involved in the mediation of metabolic resistance of many insects to organophosphate (op) insecticides. carboxylesterases underwent two divergent evolutionary events: (1) quantitative mechanism characterized by the overproduction of carboxylesterase protein; and (2) qualitative mechanism caused by changes in enzymatic properties because of mutation from glycine/alanine to aspartate at the 151 site (g/a151d) or from tryptophan to leucine at the 271 site (w271l), follo ...201526047115
resistance to cucurbit aphid-borne yellows virus in melon accession tgr-1551.the genetic control of resistance to cucurbit aphid-borne yellows virus (cabyv; genus polerovirus, family luteoviridae) in the tgr-1551 melon accession was studied through agroinoculation of a genetic family obtained from the cross between this accession and the susceptible spanish cultivar 'bola de oro'. segregation analyses were consistent with the hypothesis that one dominant gene and at least two more modifier genes confer resistance; one of these additional genes is likely present in the su ...201526075973
interspecific associations between cycloneda sanguinea and two aphid species (aphis gossypii and hyadaphis foeniculi) in sole-crop and fennel-cotton intercropping systems.aphids cause significant damage to crop plants. studies regarding predator-prey relationships in fennel (foeniculum vulgare mill.) and cotton (gossypium hirsutum l.) crops are important for understanding essential ecological interactions in the context of intercropping and for establishing pest management programs for aphids. this study evaluated the association among hyadaphis foeniculi (passerini) (hemiptera: aphididae), aphis gossypii glover (hemiptera: aphididae) and cycloneda sanguinea (l.) ...201526241862
differential life history trait associations of aphids with nonpersistent viruses in cucurbits.the diversity of vectors and fleeting nature of virus acquisition and transmission renders nonpersistent viruses a challenge to manage. we assessed the importance of noncolonizing versus colonizing vectors with a 2-yr survey of aphids and nonpersistent viruses on commercial pumpkin farms. we quantified aphid alightment using pan traps, while testing leaf samples with multiplex rt-pcr targeting cucumber mosaic virus (cmv), zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), and pa ...201526313961
impacts of transgenic poplar-cotton agro-ecosystems upon target pests and non-target insects under field conditions.poplar-cotton agro-ecosystems are the main agricultural planting modes of cotton fields in china. with increasing acres devoted to transgenic insect-resistant poplar and transgenic insect-resistant cotton, studies examining the effects of transgenic plants on target and non-target insects become increasingly important. we systematically surveyed populations of both target pests and non-target insects for 4 different combinations of poplar-cotton eco-systems over 3 years. transgenic bt cotton str ...201526345739
rna interference of odorant-binding protein 2 (obp2) of the cotton aphid, aphis gossypii (glover), resulted in altered electrophysiological responses.aphis gossypii (glover) (hemiptera: aphididae) is a highly invasive pest that feeds primarily on phloem resulting in severe economic loss to growers. a. gossypii has cosmopolitan distribution with broad host range, polyphenism, parthenogenetic mode of reproduction, vectoring abilities, and host alteration which has profound influence on its management. odorant-binding proteins (obps) in insects are involved in olfaction, playing a key role in orienting the insect for feeding or oviposition. rece ...201626432291
mirna-mediated auxin signalling repression during vat-mediated aphid resistance in cucumis melo.resistance to aphis gossypii in melon is attributed to the presence of the single dominant r gene virus aphid transmission (vat), which is biologically expressed as antibiosis, antixenosis and tolerance. however, the mechanism of resistance is poorly understood at the molecular level. aphid-induced transcriptional changes, including differentially expressed mirna profiles that correspond to resistance interaction have been reported in melon. the potential regulatory roles of mirnas in vat-mediat ...201626437210
spirotetramat resistance adaption analysis of aphis gossypii glover by transcriptomic survey.a resistant strain of the cotton aphid (sr) developed 441.26-fold and 11.97-fold resistance to spirotetramat for adult aphids and nymphs, respectively, compared with the susceptible (ss) strain. solexa sequencing technology was employed to identify differentially expressed genes (degs) in the spirotetramat-resistant cotton aphid. respective totals of 22,430,522 and 21,317,732 clean reads were obtained from sr and ss cdna libraries and assembled into 35,222 non-redundant (nr) consensus sequences. ...201526453233
intraguild predation responses in two aphidophagous coccinellids identify differences among juvenile stages and aphid densities.(1) intraguild predation (igp) can occur among aphidophagous predators thus reducing their effectiveness in controlling crop pests. among ladybirds, coccinella septempunctata l. and hippodamia variegata goeze are the most effective predators upon aphis gossypii glov., which is an economically important pest of melon. understanding their likelihood to engage in reciprocal predation is a key point for conservation of biological control. here, we aim to investigate, under laboratory conditions, the ...201426462953
economic threshold for cotton aphid (hemiptera: aphididae) on cotton in the southwestern united states.cotton aphid, aphis gossypii glover (hemiptera: aphididae), is a common pest of cotton throughout much of the world. in the united states, insecticide applications targeting cotton aphid in cotton are common in the mid-south, texas, and california. cotton aphid population dynamics data were collected from eight insecticide efficacy trials conducted in lubbock, tx, over a 4-yr period. among the field populations in the nontreated plots, the instantaneous rate of population growth averaged 0.56 ±  ...201526470321
evaluation of the synergistic effect between ethyl formate and phospine for control of aphis gossypii (homoptera: aphididae).cotton aphid, aphis gossypii glover, is known as a quarantine pest that is hard to control with short periods of fumigation with phosphine (ph(3)) or low concentrations of ethyl formate. moreover, low-temperature fumigation with ethyl formate can lead to phototoxic damage of some perishable commodities. therefore, a laboratory study was conducted to evaluate the synergistic effect of mixing ethyl formate and ph(3) for the treatment of adults and nymphs of a. gossypii. combined toxicity was obser ...201626476554
functional response of aphidoletes aphidimyza rondani (diptera: cecidomyiidae) to aphis gossypii glover (hemiptera: aphididae): effects of vermicompost and host plant cultivar.interactions between natural enemies and herbivores may be affected by application of fertilizers and different cultivars. we investigated the functional response of the predatory gall midge, aphidoletes aphidimyza rondani (diptera: cecidomyiidae) larvae to the nymphs of the melon aphid, aphis gossypii glover (hemiptera: aphididae), reared on two commonly grown cucumber cultivars in iran (khasib and karim) treated with different vermicompost/soil ratios (0:100, 10:90, 20:80, and 30:70%). based o ...201626563403
host range specificity of scymnus camptodromus (coleoptera: coccinellidae), a predator of hemlock woolly adelgid (hemiptera: adelgidae).scymnus (neopullus) camptodromus yu and liu (coleoptera: coccinellidae) was brought to the united states from china as a potential biological control agent for hemlock woolly adelgid (adelges tsugae annand) (hemiptera: adelgidae). scymnus camptodromus phenology is closely synchronized with that of a. tsugae and has several characteristics of a promising biological control agent. as a prerequisite to field release, s. camptodromus was evaluated for potential nontarget impacts. in host range studi ...201626612409
frequency detection of imidacloprid resistance allele in aphis gossypii field populations by real-time pcr amplification of specific-allele (rtpasa).the aphis gossypii glover (hemiptera: aphididae) is one of the most serious pests worldwide, and imidacloprid has been widely used to control this insect pest. just like other classes of insecticides, the resistance to imidacloprid has been found in a. gossypii. an amino acid mutation (r81t) in the nicotinic acetylcholine receptor (nachr) beta1 subunit was detected in the imidacloprid-resistant a. gossypii collected from langfang (lf) and dezhou (dz) cities. to estimate the r81t mutation frequen ...201526615144
diet-delivered dsrnas for juvenile hormone-binding protein and vacuolar atpase-h implied their potential in the management of the melon aphid (hemiptera: aphididae).rna interference is a sequence-specific gene silencing mechanism mediated by double-stranded rna (dsrna), which has been harnessed as a useful tool in devising novel insect pest management strategies for various pests such as melon aphid, aphis gossypii (glover). in the current study, we cloned and sequenced juvenile hormone-binding protein (jhbp) and vacuolar atpase subunit h (v-atpase-h) from a. gossypii. we also showed the effectiveness of diet-mediated delivery of dsrna for jhbp and v-atpase ...201626645766
biological and molecular characterization of a korean isolate of cucurbit aphid-borne yellows virus infecting cucumis species in korea.surveys of yellowing viruses in plastic tunnels and in open field crops of melon (cucumis melo cultivar catalupo), oriental melon (c. melo cultivar oriental melon), and cucumber (c. sativus) were carried out in two melon-growing areas in 2014, korea. severe yellowing symptoms on older leaves of melon and chlorotic spots on younger leaves of melon were observed in the plastic tunnels. the symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thi ...201526673519
identification of aphis gossypii glover (hemiptera: aphididae) biotypes from different host plants in north china.the cotton-melon aphid, aphis gossypii glover (hemiptera: aphididae), is a polyphagous species with a worldwide distribution and a variety of biotypes. north china is a traditional agricultural area with abundant winter and summer hosts of a. gossypii. while the life cycles of a. gossypii on different plants have been well studied, those of the biotypes of north china are still unclear.201626735973
an attractant of the aphidophagous gall midge aphidoletes aphidimyza from honeydew of aphis gossypii.many natural enemies of insects use honeydew as a volatile cue to locate hosts or prey, as an oviposition stimulant, and as an arrestant for foraging. the aphidophagous gall midge aphidoletes aphidimyza (rondani) (diptera: cecidomyiidae) has predacious larval stages and can be used to control aphid populations, especially in greenhouses. previous studies have shown that the honeydew, excreted by the aphid myzus persicae, attracts a. aphidimyza, but the crucial attractants have not been identifie ...201626757909
over-expression of cyp6a2 is associated with spirotetramat resistance and cross-resistance in the resistant strain of aphis gossypii glover.a laboratory-selected spirotetramat-resistant strain (sr) of cotton aphid developed 579-fold and 15-fold resistance to spirotetramat in adult aphids and 3rd instar nymphs, respectively, compared with a susceptible strain (ss) [26]. the sr strain developed high-level cross-resistance to alpha-cypermethrin and bifenthrin and very low or no cross-resistance to the other tested insecticides. synergist piperonyl butoxide (pbo) dramatically increased the toxicity of spirotetramat and alpha-cypermethri ...201626778436
multiple mutations on the second acetylcholinesterase gene associated with dimethoate resistance in the melon aphid, aphis gossypii (hemiptera: aphididae).the melon aphid, aphis gossypii glover (hemiptera: aphididae), is an important cosmopolitan and extremely polyphagous species capable of causing direct and indirect damage to various crops. insecticide resistance in melon aphids is of particular concern. to determine the basis of resistance, organophosphate (op)-resistant strains of a. gossypii were obtained by continuous selection with dimethoate in the laboratory, and resistance mechanisms were investigated along with susceptible strains. thre ...201626797869
establishment of polyethylene-glycol-mediated protoplast transformation for lecanicillium lecanii and development of virulence-enhanced strains against aphis gossypii.lecanicillium lecanii has been developed as a biopesticide and used in biological control of several agricultural insects. to improve fungal virulence, an optimised polyethylene glycol (peg)-mediated protoplast transformation system was established for l. lecanii. pr1a-like cuticle-degrading protease gene (cdep1) from beauveria bassiana was transferred into l. lecanii, and its resulting activity against aphis gossypii was assessed.201626800336
nbs-lrr-mediated resistance triggered by aphids: viruses do not adapt; aphids adapt via different mechanisms.aphids are serious pest on crops. by probing with their stylets, they interact with the plant, they vector viruses and when they reach the phloem they start a continuous ingestion. many plant resistances to aphids have been identified, several have been deployed. however, some resistances breaking down have been observed. in the melon, a gene that confers resistance to aphids has been deployed in some melon-producing areas, and aphid colony development on vat-carrying plants has been observed in ...201626801763
bt proteins cry1ah and cry2ab do not affect cotton aphid aphis gossypii and ladybeetle propylea japonica.plant varieties expressing the bt (bacillus thuringiensis) insecticidal proteins cry1ah and cry2ab have potential commercialization prospects in china. however, their potential effects on non-target arthropods (ntas) remain uncharacterized. the cotton aphid aphis gossypii is a worldwide pest that damages various important crops. the ladybeetle propylea japonica is a common and abundant natural enemy in many cropping systems in east asia. in the present study, the effects of cry1ah and cry2ab pro ...201626829252
host plant determines the population size of an obligate symbiont (buchnera aphidicola) in aphids.buchnera aphidicolais an obligate endosymbiont that provides aphids with several essential nutrients. though much is known about aphid-buchnera interactions, the effect of the host plant on buchnera population size remains unclear. here we used quantitative pcr (qpcr) techniques to explore the effects of the host plant on buchnera densities in the cotton-melon aphid, aphis gossypii buchneratiters were significantly higher in populations that had been reared on cucumber for over 10 years than in ...201626850304
effects of spirotetramat treatments on fecundity and carboxylesterase expression of aphis gossypii glover.spirotetramat is a novel tetramic acid-based insecticide, belonging to keto-enol pesticide family, with a novel mode of action; it interferes with lipid biosynthesis. its insecticide activity against various agricultural pest insects have been demonstrated (e.g. on myzus persicae, bemisia tabaci and tetranychus urticae). however, information available is currently limited on the efficacy of spirotetramat on the cotton aphid, aphis gossypii, a key cotton pest worldwide. we assessed the spirotetra ...201626898726
rapid assessment of insect fauna based on local knowledge: comparing ecological and ethnobiological methods.the rapid assessment of biodiversity making use of surveys of local knowledge has been successful for different biological taxa. however, there are no reports on the testing of such tools for sampling insect fauna. the present study aimed to evaluate the efficiency of different ethnobiological techniques for rapid sampling of insect fauna.201626932264
the distribution and host shifts of cotton-melon aphids in northern china.aphis gossypii glover (hemiptera: aphididae) is a serious pest of cotton in northern china. a microsatellite analysis was used to characterize the genetic structure of a. gossypii populations from different geographic, host plant, and seasonal populations in 2014. among 906 individuals, 507 multilocus genotypes were identified, with genotypic richness values of 0.07-1.00 for the populations. we observed moderate levels of genetic differentiation among geographic populations (fst = 0.103; 95% con ...201627002824
cytochrome p450 cyp6da2 regulated by cap 'n'collar isoform c (cncc) is associated with gossypol tolerance in aphis gossypii glover.cotton plants accumulate phytotoxins, such as gossypol and related sesquiterpene aldehydes, to resist insect herbivores. the survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of cytochrome p450. gossypol has an antibiotic effect on aphis gossypii, and as the concentrations of gossypol were increased in the present study, the mortality of cotton aphids increased from 4 to 28%. the fecundity of the cotton aphids exposed t ...201627005728
effects of single and dual species herbivory on the behavioural responses of three thrips species to cotton seedlings.this study investigated the olfactory responses of three thrips species [frankliniella schultzei trybom, f. occidentalis pergrande and thrips tabaci lindeman (thysanoptera: thripidae)] to cotton seedlings [gossypium hirsutum l. (malvales: malvaceae)] simultaneously damaged by different combinations of herbivores. cotton seedlings were damaged by foliar feeding tetranychus urticae koch (trombidiforms: tetranychidae), helicoverpa armigera hübner (lepidoptera: noctuidae), aphis gossypii glover (hem ...201627029603
gc×gc-tofms analysis of essential oils composition from leaves, twigs and seeds of cinnamomum camphora l. presl and their insecticidal and repellent activities.interest in essential oils with pesticidal activity against insects and pests is growing. in this study, essential oils from different parts (leaves, twigs and seeds) of cinnamomum camphora l. presl were investigated for their chemical composition, and insecticidal and repellent activities against the cotton aphid. the essential oils, obtained by hydrodistillation, were analyzed by gc×gc-tofms. a total of 96 components were identified in the essential oils and the main constituents found in the ...201627043503
characterization of the natural enemy community attacking cotton aphid in the bt cotton ecosystem in northern china.planting bt cotton in china since 1997 has led to important changes in the natural enemy communities occurring in cotton, however their specific effect on suppressing the cotton aphids (being notorious in conventional cotton ecosystem) has not been fully documented yet. we observed strong evidence for top-down control of the aphid population, e.g. the control efficiency of natural enemies on cotton aphid increased significantly in open field cages compared to exclusion cages, accounted for 60.2, ...201627075171
bacterial communities of the cotton aphid aphis gossypii associated with bt cotton in northern china.aphids are infected with a wide variety of endosymbionts that can confer ecologically relevant traits. however, the bacterial communities of most aphid species are still poorly characterized. this study investigated the bacterial diversity of the cotton aphid aphis gossypii associated with bt cotton in northern china by targeting the v4 region of the 16s rdna using the illumina miseq platform. our sequencing data revealed that bacterial communities of a. gossypii were generally dominated by the ...201627079679
a genetically novel, narrow-host-range isolate of cucumber mosaic virus (cmv) from rosemary.an isolate of cucumber mosaic virus (cmv), designated cmv-rom, was isolated from rosemary (rosmarinus officinalis) plants in several locations near avignon, france. laboratory studies showed that, unlike typical cmv isolates, cmv-rom has a particularly narrow host range. it could be transmitted by aphids aphis gossypii and myzus persicae, but with low efficacy compared to a typical cmv isolate. phylogenetic analysis of the nucleotide sequences of the cmv-rom genomic rnas shows that this isolate ...201627138549
lethal, sublethal and transgenerational effects of the novel chiral neonicotinoid pesticide cycloxaprid on demographic and behavioral traits of aphis gossypii (hemiptera: aphididae).aphis gossypii glover (hemiptera: aphididae) is a key pest in cotton crops, notably owing to its increasing resistance to commonly used pesticides. such resistance prompts for the development of integrated pest management (ipm) programs that include novel pesticides being effective against the aphid. in the present study, we assessed lethal and sublethal effects of cycloxaprid, a novel chiral neonicotinoid pesticide developed in china, on a. gossypii. the lethal concentration at 50% (lc50 ) valu ...201627168374
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