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characterization of the highly efficient sucrose isomerase from pantoea dispersa uq68j and cloning of the sucrose isomerase gene.sucrose isomerase (si) genes from pantoea dispersa uq68j, klebsiella planticola uq14s, and erwinia rhapontici wac2928 were cloned and expressed in escherichia coli. the predicted products of the uq14s and wac2928 genes were similar to known sis. the uq68j si differed substantially, and it showed the highest isomaltulose-producing efficiency in e. coli cells. the purified recombinant wac2928 si was unstable, whereas purified uq68j and uq14s sis were very stable. uq68j si activity was optimal at p ...200515746363
a novel transcriptional autoregulatory loop enhances expression of the pantoea stewartii subsp. stewartii hrp type iii secretion system.the hrp type iii secretion regulon of pantoea stewartii is regulated by a cascade involving the hrpx/hrpy two-component system, the hrps enhancer-binding protein and the hrpl alternate sigma factor. hrpxy is both constitutive and autoregulated; hrpy controls hrps; and hrps activates hrpl. these regulatory genes are arranged in the order hrpl, hrpxy and hrps and constitute three operons. this study describes a novel autoregulatory loop involving hrps. genetic experiments using a chromosomal hrps- ...200515751134
[interaction of pantoea agglomerans with the agent of basal bacteriosis of wheat].interactions between the agent of basal bacteriosis pseudomonas syringae pv. atrofaciens and epiphyte pantoea agglomerans have been studied in the laboratory and field conditions. it is shown the p. agglomerans affects the infectious process caused by p. s. pv. atrofaciens that leads to intensification of symptoms of the disease development. the epiphyte cells accumulation predominates under mutual artificial infection in the infected tissue. the higher competitive ability of the epiphyte p. agg ...200515765881
the cell density-dependent expression of stewartan exopolysaccharide in pantoea stewartii ssp. stewartii is a function of esar-mediated repression of the rcsa gene.the luxr-type quorum-sensing transcription factor esar functions as a repressor of exopolysaccharide (eps) synthesis in the phytopathogenic bacterium pantoea stewartii ssp. stewartii. the cell density-dependent expression of eps is critical for stewart's wilt disease development. strains deficient in the synthesis of a diffusible acyl-homoserine lactone inducer remain repressed for eps synthesis and are consequently avirulent. in contrast, disruption of the esar gene leads to hypermucoidy and at ...200515773989
the t-dna oncogene a4-orf8 from agrobacterium rhizogenes a4 induces abnormal growth in tobacco.the related orf8 and iaam t-dna genes from agrobacterium are each composed of two distinct parts. the 5' parts (called norf8 or niaam) encode a 200-amino-acid (aa) sequence with homology to various t-dna oncoproteins such as rolb, rolc, and 6b. the 3' parts (corf8 or ciaam) encode a 550-aa sequence with homology to iaam proteins from pseudomonas and pantoea spp. whereas iaam genes encode flavin adenine dinucleotide (fad)-dependent tryptophan 2-monooxygenases that catalyze the synthesis of indole ...200515782634
effect of plants and filter materials on bacteria removal in pilot-scale constructed wetlands.due to the lack of testing units or appropriate experimental approaches, only little is known about the removal of bacteria in constructed wetlands. however, improved performance in terms of water sanitation requires a detailed understanding of the ongoing processes. therefore, we analyzed the microbial diversity and the survival of enterobacteriaceae in six pilot-scale constructed wetland systems treating domestic wastewater: two vertical sand filters, two vertical expanded clay filters and two ...200515862336
identification of non-mutans streptococci organisms in dental plaques recovering on mitis-salivarius bacitracin agar medium.the objective of this study was to both isolate and identify non-mutans streptococci organisms (non-mso) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (msb) plates. the dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on msb. the bacteria growing on the msb plates were then identified with biochemical tests, as well as with 16s rdna cloning and sequencing techniques. our data indicated that bacteria from 30 subjects had b ...200515880098
polymicrobial peritonitis including pantoea agglomerans from teething on a catheter. 200515954524
pa 20, a semi-selective medium for isolation and enumeration of pantoea ananatis.a semi-selective medium, pa 20, was developed for the isolation and enumeration of pantoea ananatis from plant material, specifically from onion seed. the medium has a ph of 8.0 and contains nh4h2po4, k2hpo4, magnesium sulphate, nacl, d (+) arabitol, crystal violet, bromothymol blue and thallium nitrate. all p. ananatis strains from a variety of hosts produced characteristic yellow colonies in 6-7 days at 25 degrees c. plating efficiencies on pa 20 in comparison to nutrient agar ranged from 92 t ...200615979177
osmotically induced trehalose and glycine betaine accumulation improves tolerance to desiccation, survival and efficacy of the postharvest biocontrol agent pantoea agglomerans eps125.the application of the biocontrol agent pantoea agglomerans eps125 to unwounded fruits was practically ineffective for control of postharvest blue mould caused by penicillium expansum when the treatment and subsequent wounding and pathogen inoculation were separated by periods of unfavourable conditions. this was due to a rapid decrease in viability of the alocthonous introduced biocontrol agent in the intact peel surface. a system for osmoadaptation of the biocontrol agent was developed by comb ...200516002241
aggregates of resident bacteria facilitate survival of immigrant bacteria on leaf surfaces.the fate of immigrant bacterial cells on leaves under stressful conditions was determined as a function of the anatomical features and the local spatial density of resident cells at their landing site. pantoea agglomerans 299r was established on bean leaves and the survival of immigrant cells of pseudomonas fluorescens a506 and pseudomonas syringae b728a, as well as p. agglomerans itself, was determined by epifluorescence microscopy following subsequent exposure of plants to desiccation stress. ...200516003469
analysis of promoters recognized by hrpl, an alternative sigma-factor protein from pantoea agglomerans pv. gypsophilae.hrpl, an alternative sigma factor, activates the transcription of the hrp regulon by its binding to a common "hrp box" promoter. based on computational techniques, the hrp box previously was defined as a consensus bipartite cis element, 5'-ggaacc-n(15-16)-ccacnna-3'. the present report combines a quantitative in vivo assay for measuring hrp promoter activity with site-specific mutagenesis to analyze the effect of consensus and nonconsensus nucleotides on promoter activity. the analysis was carri ...200516042009
fluorescent amplified fragment length polymorphism (faflp) based molecular epidemiology of hospital infections in a tertiary care setting in hyderabad, india.bacterial isolates from respiratory and urinary tract infections in an indian hospital setting were genotyped using faflp analysis. the 77 different isolates analyzed belonged to five genera namely escherichia, staphylococcus, pseudomonas, enterobacter and pantoea. before carrying out faflp analysis all the isolates were subjected to16s-23s ribosomal rna-based species identification. cluster analysis of faflp profiles of 77 isolates generated five groups corresponding to five bacterial genera th ...200616054876
accumulation of the compatible solutes, glycine-betaine and ectoine, in osmotic stress adaptation and heat shock cross-protection in the biocontrol agent pantoea agglomerans cpa-2.the effect of modifying the water activity (a(w)) of pantoea agglomerans growth medium with the ionic solute nacl on water stress resistance, heat-shock survival and intracellular accumulation of the compatible solutes glycine-betaine and ectoine were determined.200516108915
spatial organization of dual-species bacterial aggregates on leaf surfaces.the spatial organization of cells within bacterial aggregates on leaf surfaces was determined for pair-wise mixtures of three different bacterial species commonly found on leaves, pseudomonas syringae, pantoea agglomerans, and pseudomonas fluorescens. cells were coinoculated onto bean plants and allowed to grow under moist conditions, and the resulting aggregates were examined in situ by epifluorescence microscopy. each bacterial strain could be localized because it expressed either the green or ...200516151141
phylogeny of the enterobacteriaceae based on genes encoding elongation factor tu and f-atpase beta-subunit.the phylogeny of enterobacterial species commonly found in clinical samples was analysed by comparing partial sequences of their elongation factor tu gene (tuf) and of their f-atpase beta-subunit gene (atpd). an 884 bp fragment for tuf and an 884 or 871 bp fragment for atpd were sequenced for 96 strains representing 78 species from 31 enterobacterial genera. the atpd sequence analysis exhibited an indel specific to pantoea and tatumella species, showing, for the first time, a tight phylogenetic ...200516166704
solubilization of insoluble inorganic phosphates by a novel salt- and ph-tolerant pantoea agglomerans r-42 isolated from soybean rhizosphere.to develop environment-friendly biofertilizer solubilizing insoluble phosphates, salt- and ph-tolerant, insoluble inorganic phosphate-solubilizing bacterium was isolated from soybean rhizosphere. on the basis of its physiological characteristics and vitek analysis, this bacterium was identified as pantoea agglomerans. the optimal medium composition and cultural conditions for the solubilization of insoluble phosphate by p. agglomerans r-42 were 3% (w/v) of glucose, 0.1% (w/v) of nh4no3, 0.02% (w ...200616171676
bacterial species specificity in prou osmoinducibility and nptii and lacz expression.reporter gene-based transcriptional fusions are increasingly being used to address questions in microbial ecology, with constitutively expressed fusions enabling microbe tracking and inducible fusions reporting the presence of environmental signals. to more readily apply this technology to a variety of bacterial species, we examined species specificity in the expression of three promoters of interest. a comparison of two potentially constitutive promoters, each fused to the reporter gene gfp, sh ...200416179797
endophytic bacteria in coffea arabica l.eighty-seven culturable endophytic bacterial isolates in 19 genera were obtained from coffee plants collected in colombia (n = 67), hawaii (n = 17), and mexico (n = 3). both gram positive and gram negative bacteria were isolated, with a greater percentage (68%) being gram negative. tissues yielding bacterial endophytes included adult plant leaves, various parts of the berry (e.g., crown, pulp, peduncle and seed), and leaves, stems, and roots of seedlings. some of the bacteria also occurred as ep ...200516187260
effects of yeasts and bacteria on the levels of folates in rye sourdoughs.fermentation of rye dough is often accompanied with an increase in folate content. in this study, three sourdough yeasts, candida milleri cbs 8195, saccharomyces cerevisiae ts 146, and torulaspora delbrueckii ts 207; a control, baker's yeast s. cerevisiae alko 743; and four lactobacillus spp., l. acidophilus tsb 262, l. brevis tsb 307, l. plantarum tsb 304, and l. sanfranciscensis tsb 299 originally isolated from rye sourdough were examined for their abilities to produce or consume folates. the ...200616213050
an outbreak of pantoea spp. in a neonatal intensive care unit secondary to contaminated parenteral nutrition.contaminated parenteral nutrition (pn) is an important source of infection in neonates. many organisms have been reported to cause contamination that results in outbreaks in intensive care units. the objective of this study was to investigate an outbreak caused by pantoea spp., which contaminates pn, in a neonatal intensive care unit (nicu). this was a descriptive study of an outbreak of sepsis in an nicu of a tertiary teaching hospital in malaysia. pantoea spp. infection was detected in eight p ...200516213372
the effects of long-term occupational exposure to dust from herbs.herbs are a heterogeneous group of many species with several thousand plants, which are used in large quantities in the pharmaceutical and food industries. the aim of the study was to analyse the health effects of long-term occupational exposure to dust from herbs.200616217676
purification and properties of an ice-nucleating protein from an ice-nucleating bacterium, pantoea ananatis kuin-3.an ice-nucleating protein (inp) from the extracellular ice-nucleating matter (eim) of pantoea ananatis (erwinia uredovora) kuin-3 was purified and characterized. the eim produced by the strain kuin-3 was purified by ultrafiltration, sucrose density-gradient ultracentrifugation and gel filtration. the inp was purified using of column chromatography on hydroxyapatite and superdex 200 in the nondenaturing detergent of 0.1% (w/v) triton x-100. the purified inp was composed of one subunit of 117 kda ...200316233385
rapid, specific and quantitative assays for the detection of the endophytic bacterium methylobacterium mesophilicum in plants.xylella fastidiosa is a xylem-limited bacterium that causes citrus variegated chlorosis disease in sweet orange. there is evidence that x. fastidiosa interacts with endophytic bacteria present in the xylem of sweet orange, and that these interactions, particularly with methylobacterium mesophilicum, may affect disease progress. however, these interactions cannot be evaluated in detail until efficient methods for detection and enumeration of these bacteria in planta are developed. we have previou ...200616266765
identification of anrf gene, a homology of admm of andrimid biosynthetic gene cluster related to the antagonistic activity of enterobacter cloacae b8.to identify the gene (s) related to the antagonistic activity of enterobacter cloacae b8 and to elucidate its antagonistic mechanism.200516273642
reliability of diagnostic techniques for erwinia amylovora, the causative agent of fire blight disease.a total of 20 putative strains of erwinia amylovora originating from 11 samples of host plants with symptoms of fire blight were analyzed in detail using commercial polyclonal antibodies in immunochemical tests. fourteen strains reacted negatively in all tests; 6 strains reacted positively with a polyclonal antibody for pta-elisa (plate-trapped antigen-enzyme linked immunosorbent assay) at a concentration corresponding to a620 = 0.1, while at a620 readings of 0.01 and 0.001 the results were nega ...200516295660
characterization of microbial contamination in united states air force aviation fuel tanks.bacteria and fungi, isolated from united states air force (usaf) aviation fuel samples, were identified by gas chromatograph fatty acid methyl ester (gc-fame) profiling and 16s or 18s rrna gene sequencing. thirty-six samples from 11 geographically separated usaf bases were collected. at each base, an above-ground storage tank, a refueling truck, and an aircraft wing tank were sampled at the lowest sample point, or sump, to investigate microbial diversity and dispersion within the fuel distributi ...200616328508
diversity of carotenoid synthesis gene clusters from environmental enterobacteriaceae strains.eight enterobacteriaceae strains that produce zeaxanthin and derivatives of this compound were isolated from a variety of environmental samples. phylogenetic analysis showed that these strains grouped with different clusters of erwinia type strains. four strains representing the phylogenetic diversity were chosen for further characterization, which revealed their genetic diversity as well as their biochemical diversity. the carotenoid synthesis gene clusters cloned from the four strains had thre ...200516332796
engineering of solvent-tolerant pseudomonas putida s12 for bioproduction of phenol from glucose.efficient bioconversion of glucose to phenol via the central metabolite tyrosine was achieved in the solvent-tolerant strain pseudomonas putida s12. the tpl gene from pantoea agglomerans, encoding tyrosine phenol lyase, was introduced into p. putida s12 to enable phenol production. tyrosine availability was a bottleneck for efficient production. the production host was optimized by overexpressing the arof-1 gene, which codes for the first enzyme in the tyrosine biosynthetic pathway, and by rando ...200516332806
coexistence among epiphytic bacterial populations mediated through nutritional resource partitioning.the levels of coexistence between pseudomonas syringae and various nonpathogenic epiphytic species in the phyllosphere of beans (phaseolus vulgaris) were assessed by using replacement series. the epiphytic species pseudomonas fluorescens, pantoea agglomerans, stenotrophomonas maltophilia, and methylobacterium organophilum were all capable of exhibiting higher levels of coexistence with p. syringae than was observed with a near-isogenic p. syringae strain pair. the ecological similarity of the ep ...199416349462
specificity of acyl-homoserine lactone synthases examined by mass spectrometry.many gram-negative bacteria produce a specific set of n-acyl-l-homoserine-lactone (ahl) signaling molecules for the purpose of quorum sensing, which is a means of regulating coordinated gene expression in a cell-density-dependent manner. ahls are produced from acylated acyl-carrier protein (acyl-acp) and s-adenosyl-l-methionine by the ahl synthase enzyme. the appearance of specific ahls is due in large part to the intrinsic specificity of the enzyme for subsets of acyl-acp substrates. structural ...200616385066
cloning and expression of a xylitol-4-dehydrogenase gene from pantoea ananatis.the pantoea ananatis atcc 43072 mutant strain is capable of growing with xylitol as the sole carbon source. the xylitol-4-dehydrogenase (xdh) catalyzing the oxidation of xylitol to l-xylulose was isolated from the cell extract of this strain. the n-terminal amino acid sequence of the purified protein was determined, and an oligonucleotide deduced from this peptide sequence was used to isolate the xylitol-4-dehydrogenase gene (xdh) from a p. ananatis gene library. nucleotide sequence analysis rev ...200616391066
responses of the anaerobic bacterial community to addition of organic c in chromium(vi)- and iron(iii)-amended microcosms.chromium (vi) is toxic to microorganisms and can inhibit the biodegradation of organic pollutants in contaminated soils. we used microcosms amended with either glucose or protein (to drive bacterial community change) and fe(iii) (to stimulate iron-reducing bacteria) to study the effect of various concentrations of cr(vi) on anaerobic bacterial communities. microcosms were destructively sampled based on microbial activity (measured as evolution of co2) and analyzed for the following: (i) dominant ...200616391100
health effects of exposure to herb dust in valerian growing farmers.the aim of the present study was to determine the health status of farmers cultivating valerian (valeriana officinalis l.) and occupationally exposed to dust from this plant. a group of 75 valerian growing farmers were examined. as a reference group, 50 urban dwellers, not exposed to any kind of organic dust were examined. all people were interviewed for the presence of work-related symptoms and subjected to physical and spirometric examinations. skin prick tests were conducted with 4 microbial ...200516457481
exposure to airborne microorganisms, dust and endotoxin during processing of peppermint and chamomile herbs on farms.the aim of this study was to determine the levels of microorganisms, dust and endotoxin in the air during processing of peppermint (mentha piperita) and chamomile (matricaria recutita) by herb farmers, and to examine the species composition of airborne microflora. air samples were collected on glass fibre filters by use of personal samplers on 13 farms owned by herb cultivating farmers, located in lublin province (eastern poland). the concentrations of total viable microorganisms (bacteria + fun ...200516457486
immunostimulative effects of repeated inhalation exposure to microvesicle-bound endotoxin of pantoea agglomerans.rabbits exposed repeatedly to aerosols of endotoxin-containing microvesicles (ecmv) of the outer membrane of the pantoea agglomerans strain isolated from airborne grain dust showed a large increase in the concentration of circulating cytokines: total interferon (ifn), interleukin-1 alpha (il-1 alpha), and tumor necrosis factor alpha (tnf alpha). the increase was significantly higher compared to animals exposed to control saline (p < 0.001). aerosol exposure to ecmv also induced the formation of ...200516457487
comparative anatomy of gall development on gypsophila paniculata induced by bacteria with different mechanisms of pathogenicity.galls induced on gypsophila paniculata by pantoea agglomerans pv. gypsophilae (pag) and agrobacterium tumefaciens (at), bacteria with different mechanisms of pathogenicity, were compared morphologically and anatomically. the pathogenicity of pag is dependent on the presence of an indigenous plasmid that harbors hrp gene cluster, genes encoding hop virulence proteins and biosynthetic genes for auxin (iaa) and cytokinins (cks), whereas that of at involves host transformation. the pag-induced gall ...200616477460
biosynthesis of novel carotenoid families based on unnatural carbon backbones: a model for diversification of natural product pathways.we show that the c40 carotenoid desaturase crti from pantoea ananatis (erwinia uredovora) is capable of desaturating unnaturally long c45 and c50 carotenoid backbones in recombinant e. coli. desaturation step number in these pathways is not very specific, and at least ten new c45 and c50 carotenoids were synthesized. we also present evidence for a novel asymmetric c40 backbone formed by the condensation of farnesyl diphosphate (c15pp) with farnesylgeranyl diphosphate (c25pp), and the subsequent ...200616478677
microbial quality of commercial 'golden delicious' apples throughout production and shelf-life in lleida (catalonia, spain).'golden delicious' apples taken from different points throughout the production chain and shelf-life were analysed for enterobacteriaceae, thermotolerant coliforms, e. coli, e. coli o157:h7 and salmonella spp. a total of 36 samples picked up in the orchard, 36 after storage and handling in the packinghouses and 144 from different retail stores were analysed using standardised techniques. enterobacteriaceae counts were very variable. pantoea spp. was the main genera isolated. the percentage of sa ...200616488042
a chromogenic plating medium for the isolation and identification of enterobacter sakazakii from foods, food ingredients, and environmental sources.a chromogenic agar, r&f enterobacter sakazakii chromogenic plating medium (espm), was developed for isolating presumptive colonies of e. sakazakii from foods and environmental sources. espm contains two chromogenic substrates (5-bromo-4-chloro-3-indoxyl-alpha-d-glucopyranoside and 5-bromo-4-chloro-3-indoxyl-beta-d-cellobioside), three sugars (sorbitol, d-arabitol, and adonitol), a ph indicator, and inhibitors (bile salts, vancomycin, and cefsulodin), which all contribute to its selectivity and d ...200616496571
the efficacy of electrolysed oxidising water for inactivating spoilage microorganisms in process water and on minimally processed vegetables.the efficacy of electrolysed oxidising water (eow) for inactivating spoilage microorganisms in process water and on minimally processed vegetables was investigated. the direct effect of eow on three important spoilage bacteria namely; pseudomonas fluorescens, pantoea agglomerans or rahnella aquatilis was determined by inoculating tap water or "artificial process water" with approximately 8 log cfu/ml pure culture and electrolysing the resultant solutions. the three bacteria were each reduced to ...200616504328
pantoea agglomerans sepsis after rotavirus gastroenteritis. 200616511401
the phytopathogen dickeya dadantii (erwinia chrysanthemi 3937) is a pathogen of the pea aphid.dickeya dadantii (erwinia chrysanthemi) is a phytopathogenic bacterium causing soft rot diseases on many crops. the sequencing of its genome identified four genes encoding homologues of the cyt family of insecticidal toxins from bacillus thuringiensis, which are not present in the close relative pectobacterium carotovorum subsp. atrosepticum. the pathogenicity of d. dadantii was tested on the pea aphid acyrthosiphon pisum, and the bacterium was shown to be highly virulent for this insect, either ...200616517643
isolation of enterobacter sakazakii from stable flies, stomoxys calcitrans l. (diptera: muscidae).enterobacter sakazakii is an opportunistic foodborne pathogen that causes meningitis, enterocolitis, and sepsis, primarily in immunocompromised infants. previously, it was suggested that stable flies, stomoxys calcitrans, were a vector or reservoir of this pathogen. in our study, by means of a culturing approach combined with 16s rdna pcr-restriction fragment length polymorphism genotyping and sequencing, we screened 928 individual stable flies collected in kansas and florida. two stable flies ( ...200616541702
structure and protective effect of exopolysaccharide from p. agglomerans strain kfs-9 against uv radiation.the water-soluble exopolysaccharide (wseps) from pantoea agglomerans strain kfs-9 isolated from mangrove forest was prepared by removing bacterial cell from the fermentation liquid following by concentration and cold ethanol precipitation of the supernatant. the polysaccharide material was purified by gel permeation chromatography on a sephacryl s-300hr column and characterized using chemical and spectral methods. the results show that wseps is protein-bound polysaccharide, and it is composed of ...200716580187
quorum-sensing regulation governs bacterial adhesion, biofilm development, and host colonization in pantoea stewartii subspecies stewartii.the phytopathogenic bacterium pantoea stewartii subsp. stewartii synthesizes stewartan exo/capsular polysaccharide (eps) in a cell density-dependent manner governed by the esai/esar quorum-sensing (qs) system. this study analyzes biofilm development and host colonization of the wt and qs regulatory mutant strains of p. stewartii. first, we show that the cell density-dependent synthesis of stewartan eps, governed by the esai/esar qs system, is required for proper bacterial adhesion and developmen ...200616585516
spent growth medium of pantoea agglomerans primes wheat suspension cells for augmented accumulation of hydrogen peroxide and enhanced peroxidase activity upon elicitation.induced disease resistance in plants is based on multiple mechanisms, including cell "priming", i.e. an enhancement of the capacity to mobilize cellular defense responses upon pathogen attack. potent inducers of priming are, for example, salicylic acid, synthetic compounds such as a benzothiadiazole, and certain rhizosphere bacteria. while priming is well characterized for a number of dicot plants, only few cases of priming are documented in monocots. here, we report that the spent growth medium ...200616596409
characterization of bacterial beta-carotene 3,3'-hydroxylases, crtz, and p450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in escherichia coli.beta-carotene hydroxylase (crtz) is one of rate-limiting enzymes for astaxanthin production. a complementation analysis was conducted using escherichia coli transformants to compare the catalytic efficiency of bacterial crtz from brevundimonas sp. sd212, paracoccus sp. pc1 (formerly known as alcaligenes sp. pc-1), paracoccus sp. n81106 (agrobacterium aurantiacum), pantoea ananatis (erwinia uredovora 20d3), marine bacterium p99-3, and p450 monooxygenase (cyp175a1) from thermus thermophilus hb27. ...200616614859
rice endophyte pantoea agglomerans ys19 promotes host plant growth and affects allocations of host photosynthates.the aims of the study were to identify the effects of rice endophyte pantoea agglomerans ys19 on host plant growth and allocations of photosynthates.200616629994
the clinical picture of neonatal infection with pantoea species.pantoea infections are uncommon in humans. most reports have involved adults or children after thorn injuries. there are only a few reports of systemic infections with pantoea. this is the first report of the clinical picture of systemic pantoea spp. infection in neonates as observed during an outbreak in a neonatal intensive care unit caused by infected parenteral nutrition solutions. even though detected early, the infections had a fulminant course, causing septicemic shock and respiratory fai ...200616632913
dominance of lysobacter sp. in the rhizosphere of two coastal sand dune plant species, calystegia soldanella and elymus mollis.bacterial diversity in the rhizosphere of beach morning glory (calystegia soldanella) and wild rye (elymus mollis), two of the major plant species inhabiting the coastal sane dune in tae-an, korea, was studied by the analysis of community 16s rrna gene clones. the amplified rdna restriction analysis (ardra) of the clones using haeiii exhibited significant differences in the community composition between the two plant species as well as regional differences, but also identified a specific ardra p ...200616652206
pantoea peritonitis in a patient receiving chronic ambulatory peritoneal dialysis.pantoea agglomerans is usually the most common organism transmitted through plant thorn injuries. this report is of a female patient maintained on chronic ambulatory peritoneal dialysis (capd) who developed peritonitis attributed to p. agglomerans. peritonitis is an uncommon complication of p. agglomerans and there is no previous report of peritonitis associated with this organism in a capd patient. the source of infection was thought to be due to rose-thorn injury. antibiotic therapy with cefta ...200616669968
production of quorum-sensing-related signal molecules by epiphytic bacteria inhabiting wheat heads.the production of quorum-sensing-related signal molecules (qsrms) among culturable bacteria comprising the community on wheat heads was investigated. the taxonomic position of 186 bacterial isolates obtained from ten heads was inferred based on 16s rrna gene sequences, and their qsrm production was determined using two bioreporter strains of n-acylhomoserine lactones. approximately 33% of isolates produced qsrms, though the proportion of qsrm-producing isolates on a wheat head was significantly ...200616699565
confirmation of e. coli among other thermotolerant coliform bacteria in paper mill effluents, wood chips screening rejects and paper sludges.paper sludges are solid wastes material generated from the paper production, which have been characterized for their chemical contents. some are rich in wood fiber and are a good carbon source, for example the primary and de-inking paper sludges. others are made rich in nitrogen and phosphorus by pressing the activated sludge, resulting from the biological water treatments, with the primary sludge, yielding the combined paper sludge. still, in the absence of sanitary effluents very few studies h ...200616714043
characterization of a collection of enterobacter sakazakii isolates from environmental and food sources.enterobacter sakazakii has emerged as a rare cause of neonatal meningitis, septicemia and enterocolitis. contaminated infant milk formula (imf) has been identified as one infection route. a small number of clinical outbreaks have been epidemiologically linked to imf contaminated post-pasteurization during manufacture and/or mishandled when reconstituted. currently no agreed standardized typing protocol has been developed to trace e. sakazakii. the objectives of this study were to apply biochemic ...200616730386
characterization of fe(iii)-reducing enrichment cultures and isolation of fe(iii)-reducing bacteria from the savannah river site, south carolina.the savannah river site, south carolina (srs), has been subjected to heavy metal and radionuclide contamination. dissimilatory fe(iii)-reducing bacteria, by reducing insoluble fe(iii) to soluble fe(ii), may enhance contaminant mobility through subsurface environments. in order to investigate populations of the indigenous iron-reducing microbes from the srs, duplicate enrichment cultures were initiated using a 10% inoculum of 7 sediment/soil samples, and serial dilutions were made into wolfe's mi ...200616730954
[an outbreak of fatal pantoea infections in newborn infants, caused by contaminated infusion solutions]. 200616736824
anopheles gambiae (diptera: culicidae) oviposition in response to agarose media and cultured bacterial volatiles.anopheles gambiae s.s. giles accepted a range of agarose gels, varying from 0.5 to 8% (wt:vol), for oviposition; laid more eggs on 0.5% agarose gels than on moist filter paper or on drier agarose; and laid equal numbers of eggs on 0.5% agarose gels and distilled water. larvae hatched on agarose gel substrates and crawled onto the surface of moist agarose gels, but they tended only to burst the egg cap and remain within the egg case on drier gels. a mixture of cultured bacteria, originating from ...200616739407
the rcsa promoter of pantoea stewartii subsp. stewartii features a low-level constitutive promoter and an esar quorum-sensing-regulated promoter.the upstream region of the pantoea stewartii rcsa gene features two promoters, one for constitutive basal-level expression and a second autoregulated promoter for induced expression. the esar quorum-sensing repressor binds to a site centered between the two promoters, blocking transcription elongation from the regulated promoter under noninducing conditions.200616740966
characterisation of prototype nurmi cultures using culture-based microbiological techniques and pcr-dgge.undefined nurmi-type cultures (ntcs) have been used successfully to prevent salmonella colonisation in poultry for decades. such cultures are derived from the caecal contents of specific-pathogen-free birds and are administered via drinking water or spray application onto eggs in the hatchery. these cultures consist of many non-culturable and obligately anaerobic bacteria. due to their undefined nature it is difficult to obtain approval from regulatory agencies to use these preparations as direc ...200616814892
molecular characterization of pantoea stewartii subsp. stewartii hrpy, a conserved response regulator of the hrp type iii secretion system, and its interaction with the hrps promoter.pantoea stewartii subsp. stewartii is a bacterial pathogen of corn. its pathogenicity depends on the translocation of effector proteins into host cells by the hrp type iii secretion system. we previously showed by genetic analysis that the hrpx sensor kinase and the hrpy response regulator are at the head of a complex cascade of regulators controlling hrp/hrc secretion and wts effector genes. this cascade also includes the hrps response regulator and the hrpl alternative sigma factor. these regu ...200616816181
volatile metabolite production of spoilage micro-organisms on a mixed-lettuce agar during storage at 7 degrees c in air and low oxygen atmosphere.this paper describes the volatile metabolite production of spoilage bacteria (pantoea agglomerans and rahnella aquatilis) and spoilage yeasts (pichia fermentans and cryptococcus laurentii), previously isolated from mixed lettuce, on a simulation medium of shredded mixed lettuce (mixed-lettuce agar) both under air conditions and modified atmosphere (ma)-conditions at 7 degrees c. these latter conditions simulated the equilibrium modified atmosphere packaging, which is used to extend the shelf-lif ...200616860421
exopolysaccharides of pantoea agglomerans have different priming and eliciting activities in suspension-cultured cells of monocots and dicots.induced disease resistance of plants is often associated with an enhanced capacity to activate cellular defense responses to pathogen attack, named the "primed" state of the plant. exopolysaccharides of pantoea agglomerans have recently been reported as the first priming active component of bacterial origin in wheat cells. we now show that pantoea exopolysaccharides also prime rice cells for better elicitation of a rapid oxidative burst. in contrast, in tobacco and parsley cell cultures pantoea ...200616860795
diverse endophytic bacteria isolated from a leguminous tree conzattia multiflora grown in mexico.conzattia multiflora is a leguminous tree present only in mexico and guatemala. there is no record about its symbiotic or pathogenic microbes. in this study, we found that numerous bacteria with 10(4)-10(6) individuals per gram of fresh epidermis were distributed in the tissue of this plant. all the bacteria isolated from the conzattia epidermis were gram-negative, facultative anaerobic rods and formed yellow or colorless colonies. they were identified as endophytes by inoculation tests. some of ...200616862424
antimicrobial activity of vanillin against spoilage microorganisms in stored fresh-cut mangoes.the antimicrobial activity of vanillin against four bacteria (pantoea agglomerans, aeromonas enteropelogenes, micrococcus lylae, and sphingobacterium spiritovorun), four fungi (alternaria sp., aspergillus sp., penicillium sp., and fusarium sp.), and three unidentified yeasts isolated from spoiling fresh-cut mango slices was verified in laboratory media adjusted to ph 5.0. mics of vanillin against the fungi (12.5 to 13.3 mm), bacteria (10 to 13.3 mm), and yeasts (5.0 to 6.7 mm) indicated that all ...200616865911
nature of bacteria found on some wards in sultan qaboos university hospital, oman.this study aims to determine what objects lying in the hospital environment or brought in from outside contribute to the introduction of bacteria associated with nosocomial infections. one hundred swab specimens collected from children's toys, sinks, door handles, telephone handsets and flowers brought into the hospital were plated on different culture media. colonial growth on the media was purified and identified subsequently using standard bacteriological methods. of the 100 samples cultured, ...200616871995
domain switching and host recognition.the collective function of secreted pathogen effector molecules is to enhance the virulence and avirulence activity of the pathogen during the infection of its host. while the activity of a majority of pathogen effectors is unknown, several classes of effector molecules have been well characterized. among these include proteins which function to modulate host defences either through proteolysis, post-translational modifications, or by directly manipulating the host transcriptional machinery that ...200616879410
the type iii effectors hsvg and hsvb of gall-forming pantoea agglomerans determine host specificity and function as transcriptional activators.pantoea agglomerans pv. gypsophilae (pag) elicits galls on gypsophila and a hypersensitive response on beet, whereas p. agglomerans pv. betae (pab) induces galls on both beet and gypsophila. the pathogenicity of both pathovars is dependent on the presence of a plasmid harbouring type iii secretion system (ttss) components and effectors. the hsvg ttss effectors of pag (hsvg-pag) and pab (hsvg-pab) determine the host specificity of both pathovars on gypsophila. here we describe a novel hsvg homolo ...200616879413
structural elucidation and biological activity of acyl-homoserine lactones from the phytopathogen pantoea ananatis serrano 1928.in gram-negative bacteria, the acyl-homoserine lactones (acyl-hsls) are the main signaling substances employed in cell-to-cell communication systems. this paper describes the chemical characterization of acyl-hsls produced by the worldwide-spread phytopathogen pantoea ananatis (serrano 1928) by using gas chromatography-electron impact mass spectrometry. the absolute configuration of the major identified substance, (s)-(--)-n-hexanoyl-hsl, was determined with gas chromatography-flame ionization d ...200616900431
a biosynthetic gene cluster for the acetyl-coa carboxylase inhibitor andrimid.increasing bacterial resistance to antibiotics with conventional targets has focused attention on antibiotics with unconventional targets. one promising candidate, the acetyl-coa carboxylase (acc) inhibitor andrimid, is a potent, broad-spectrum antibiotic with high selectivity for prokaryotic acc. here, we report the use of a dna-based approach to clone the andrimid biosynthetic gene cluster from pantoea agglomerans, yielding a cosmid that confers robust andrimid production on escherichia coli. ...200616910643
[bacteremia caused by pantoea agglomerans]. 200616913075
survival of cephalosporin-resistant enterobacteriaceae on fingers.five strains of enterobacter species (n=4) and pantoea species (n=1) resistant to third-generation cephalosporins and isolated from clusters of 3-25 premature infants and small children and 5 strains (4 enterobacter strains and 1 pantoea strain) with the same resistance pattern that were isolated from 1 premature infant or small child each were inoculated on the fingertips of 10 volunteer study participants to test whether survival on fingertips is correlated with horizontal transmission. althou ...200616941326
effectiveness of two-sided uv-c treatments in inhibiting natural microflora and extending the shelf-life of minimally processed 'red oak leaf' lettuce.the use of uv-c radiation treatments to inhibit the microbial growth and extend the shelf-life of minimally processed 'red oak leaf' lettuce was investigated. initially, uv-c resistance of 20 bacterial strains from different genera often associated with fresh produce (enterobacter, erwinia, escherichia, leuconostoc, pantoea, pseudomonas, rahnela, salmonella, serratia and yersinia) were tested in vitro. most of the bacterial strains were inhibited with the minimum dose (30 j m(-2)). erwinia carot ...200616943010
pseudooutbreak of pantoea species bacteremia associated with contaminated cotton pledgets.a total of 22 isolates of pantoea strains, unusual causative agents of clinical infection, was isolated from blood cultures from 9 patients and 1 ear swab from 1 of the patients within a period of 1 month in a tertiary-care hospital. pseudooutbreak was suspected because specimens were collected from a limited number of places and the patients did not show consistent signs or symptoms of bacterial sepsis. enterobacterial repetitive intergenic consensus (eric) polymerase chain reaction (pcr) and p ...200616945691
improving low water activity and desiccation tolerance of the biocontrol agent pantoea agglomerans cpa-2 by osmotic treatments.to study the improvement of tolerance to low water activity (aw) and desiccation during spray drying in pantoea agglomerans cells subjected to mild osmotic stress during growth.200616968304
evaluation of the bd phoenix automated microbiology system for identification and antimicrobial susceptibility testing of enterobacteriaceae.we evaluated the accuracy of the bd phoenix system for the identification (id) and antimicrobial susceptibility testing (ast) of 251 isolates of the family enterobacteriaceae representing 31 species. organisms were inoculated onto the phoenix panel according to the manufacturer's instructions. the results from conventional biochemical tests were used for the reference method for id. agar dilution, performed according to the clsi guidelines, was the reference ast method. essential and categorical ...200617021074
wtse, an avre-family effector protein from pantoea stewartii subsp. stewartii, causes disease-associated cell death in corn and requires a chaperone protein for stability.the pathogenicity of pantoea stewartii subsp. stewartii to sweet corn and maize requires a hrp type iii secretion system. in this study, we genetically and functionally characterized a disease-specific (dsp) effector locus, composed of wtse and wtsf, that is adjacent to the hrp gene cluster. wtse, a member of the avre family of effector proteins, was essential for pathogenesis on corn and was complemented by dspa/e from erwinia amylovora. an intact c-terminus of wtse, which contained a putative ...200617022173
nitrogen-fixing chemo-organotrophic bacteria isolated from cyanobacteria-deprived lichens and their ability to solubilize phosphate and to release amino acids and phytohormones.cyanobacteria-deprived lichens of the species canoparmelia caroliniana, canoparmelia crozalsiana, canoparmelia texana, parmotrema sancti-angeli and parmotrema tinctorum were screened for the presence of chemo-organotrophic nitrogen-fixing bacteria.200617040231
identification of genetic markers to distinguish the virulent and avirulent subspecies of pantoea stewartii by comparative proteomics and genetic analysis.pantoea stewartii subsp. stewartii (pnss), the causal agent of stewart's bacterial wilt and leaf blight of maize and sweet corn, is one of the quarantine pathogens in many countries and regions. in contrast, p. stewartii subsp. indologenes (pnsi), the closely related subspecies of pnss, is avirulent on these plants. in this study, the protein expression profiles of these two subspecies were compared using two-dimensional gel electrophoresis analysis. twenty-one unique protein spots consistently ...200717086414
the survival of pathogens in soil treated with wastewater sludge and in potatoes grown in such soil.the prevalence of pathogens on potatoes (solanum tuberosum) grown in soil amended with a pathogen rich wastewater sludge was investigated. bacteria of the family enterobacteriaceae are important pathogens causing intestinal and systemic illness of humans and other animals. type b sludge was used. sludges investigated are the high metal and the low metal sludges. microorganisms in the sludge-amended soil were using culture-based technique. salmonella and e. coli were observed in tested soil sampl ...200617087382
autoinducer-2 of the fire blight pathogen erwinia amylovora and other plant-associated bacteria.autoinducers are important for cellular communication of bacteria. the luxs gene has a central role in the synthesis of autoinducer-2 (ai-2). the gene was identified in a shotgun library of erwinia amylovora and primers designed for pcr amplification from bacterial dna. supernatants of several erwinia amylovora strains were assayed for ai-2 activity with a vibrio harveyi mutant and were positive. many other plant-associated bacteria also showed ai-2 activity such as erwinia pyrifoliae and erwini ...200717092294
activities and survival of endophytic bacteria in white clover (trifolium repens l.).in this study, the genera, abundance, and activities of endophytic bacteria in field-grown white clover (trifolium repens) and the fate of introduced antibiotic-tolerant bacteria in white clover tissues were investigated. pseudomonas, pantoea, and corynebacterium were the most frequently isolated endophytic bacteria genera, whereas xanthomonas, microbacterium, and cellulomonas occurred less frequently. the average bacterial populations in stolons and roots were approximately 100,000 colony-formi ...200617110977
engineering the lycopene synthetic pathway in e. coli by comparison of the carotenoid genes of pantoea agglomerans and pantoea ananatis.the lycopene synthetic pathway was engineered in escherichia coli using the carotenoid genes (crte, crtb, and crti) of pantoea agglomerans and pantoea ananatis. e. coli harboring the p. agglomerans crt genes produced 27 mg/l of lycopene in 2yt medium without isopropyl-beta-d: -thiogalactopyranoside (iptg) induction, which was twofold higher than that produced by e. coli harboring the p. ananatis crt genes (12 mg/l lycopene) with 0.1 mm iptg induction. the crt genes of p. agglomerans proved bette ...200717115209
bacteria associated with orchid roots and microbial production of auxin.associative bacteria of terrestrial (paphiopedilum appletonianum) and epiphytic (pholidota articulata) tropical orchids were investigated. microbial community of epiphytic plant differed from that of the terrestrial one. streptomyces, bacillus, pseudomonas, burkholderia, erwinia and nocardia strains populated paphiopedilum roots, whereas pseudomonas, flavobacterium, stenotrophomonas, pantoea, chryseobacterium, bacillus, agrobacterium, erwinia, burkholderia and paracoccus strains colonized pholid ...200717140781
role of pantoea agglomerans in opportunistic bacterial seed and boll rot of cotton (gossypium hirsutum) grown in the field.to investigate the aetiology of seed and boll rot of cotton grown in south carolina (sc).200717184328
enterobacteriaceae in dehydrated powdered infant formula manufactured in indonesia and malaysia.to determine the occurrence of salmonella and shigella in infant formula from southeast asia, 74 packages of dehydrated powdered infant follow-on formula (recommended age, > 4 months) from five different manufacturers, four from indonesia and one from malaysia, were analyzed. none of the 25-g test portions yielded salmonella or shigella. however, further identification of colonies growing on selective media used for salmonella and shigella detection revealed the frequent occurrence of several ot ...200617186672
investigations into the in vitro antimicrobial activity and mode of action of the phenazine antibiotic d-alanylgriseoluteic acid.d-alanylgriseoluteic acid (aga) is a potent antimicrobial phenazine compound produced by pantoea agglomerans (erwinia herbicola) eh1087. susceptibility tests against a range of microbes indicated that aga had a broad spectrum of antimicrobial activity and was particularly active against gram-positive pathogens. comparison of the in vitro efficacy of aga with eight other antibiotics against 119 clinical isolates of streptococcus pneumoniae demonstrated that all were inhibited by low concentration ...200717189100
metabolic engineering of carotenoid biosynthesis in escherichia coli by ordered gene assembly in bacillus subtilis.we attempted to optimize the production of zeaxanthin in escherichia coli by reordering five biosynthetic genes in the natural carotenoid cluster of pantoea ananatis. newly designed operons for zeaxanthin production were constructed by the ordered gene assembly in bacillus subtilis (ogab) method, which can assemble multiple genes in one step using an intrinsic b. subtilis plasmid transformation system. the highest level of production of zeaxanthin in e. coli (820 microg/g [dry weight]) was obser ...200717194842
legionella and other gram-negative bacteria in potable water from various rural and urban sources.a total of 107 potable water samples were collected from various rural and urban sources located in the lublin region (eastern poland). 54 samples from rural sources comprised 32 samples of untreated well water and 22 samples of treated (chlorinated) tap water from rural dwellings distributed by the municipal water supply system (mwss). 53 samples of treated water from urban sources were supplied by the city of lublin mwss. they comprised: 11 samples of tap water from offices and shops, 8 sample ...200617196009
biosurfactant production by antarctic facultative anaerobe pantoea sp. during growth on hydrocarbons.the facultative anaerobe pantoea sp. strain a-13, isolated from ornithogenic soil of dewart island (frazier islands), antarctica, produced glycolipid biosurfactants when grown on n-paraffins or kerosene as the sole source of carbon and energy. hemolysis of erythrocytes, growth inhibition of bacillus subtilis, and thin-layer chromatography studies have suggested that the secreted glycolipids are rhamnolipids. glycolipids produced by kerosene-grown cells decreased the surface tension at the air-wa ...200717211540
characterization of the bacterial microflora of the tympanic cavity of eastern box turtles with and without aural abscesses.aerobic bacterial cultures of the tympanic cavity of the middle ear were performed in eight eastern box turtles (terrapene carolina carolina) with aural abscesses and 15 eastern box turtles without aural abscesses (controls) that were admitted to the wildlife center of virginia, virginia, usa during 2003. twenty-two bacterial isolates were identified from 17 turtles including 10 gram-negative and 12 gram-positive bacteria. ten of 15 control animals had bacterial growth, resulting in identificati ...200617255456
acyl-homoserine lactones produced by pantoea sp. isolated from the "maize white spot" foliar disease.the "maize white spot" foliar disease is a problem of increasing importance to brazilian maize crops. a bacterium isolated from water-soaked lesions from infected maize leaves was pathogenic in biological assays in vivo. it was identified as a gram-negative, nonsporulating, facultative anaerobic bacterium, belonging to the genus pantoea. chemical study of the extracts from bacterial cultivation media allowed the identification of (s)-(-)-n-butanoyl-homoserine lactone and trace amounts of n-hexan ...200717256964
applications of lipopolysaccharide derived from pantoea agglomerans (ip-pa1) for health care based on macrophage network theory.innate immunity is a universal prophylactic system which all multi-cellular animals possess. macrophages are the cells that play the central role in the innate immune system. in 1991, we discovered a substance in a water extract of wheat flour that activated macrophages after oral or intradermal administration. the active substance was lipopolysaccharide (lps), which is derived from the cell walls of pantoea agglomerans, gram-negative bacteria that grows symbiotically with wheat. we named the su ...200617270712
a faflp system for the improved identification of plant-pathogenic and plant-associated species of the genus pantoea.the majority of pantoea species are either plant-pathogenic or plant-associated and cause a wide variety of symptoms on a range of hosts. identification of pantoea species is difficult due to minor differences in phenotypic characteristics between them and related enterobacteriaceae. fluorescent amplified fragment length polymorphism (faflp) analysis was investigated for use as a rapid, molecular-based identification technique to the species level of the genus pantoea. following analysis of the ...200717321713
seizure associated with total parenteral nutrition-related hypermanganesemia.the trace element manganese is usually supplied when total parenteral nutrition is used. however, long-term parenteral administration of manganese, which bypasses the normal regulatory mechanism, may cause hypermanganesemia. manganese poisoning presents clinically with parkinsonian-like symptoms and psychological changes. seizures are a rare presentation of this disease. this report describes a 10-year-old female who had received total parenteral nutrition for 3 months because of short bowel syn ...200717352953
culturable leaf-associated bacteria on tomato plants and their potential as biological control agents.culturable leaf-associated bacteria inhabiting a plant have been considered as promising biological control agent (bca) candidates because they can survive on the plant. we investigated the relationship between bacterial groups of culturable leaf-associated bacteria on greenhouse- and field-grown tomato leaves and their antifungal activities against tomato diseases in vitro and in vivo. in addition, the isolated bacteria were analyzed for n-acyl-homoserine lactone (ahl) and indole-3-acetic acid ...200717356949
culturable bacteria present in the fluid of the hooded-pitcher plant sarracenia minor based on 16s rdna gene sequence data.the culturable microbial community within the pitcher fluid of 93 sarracenia minor carnivorous plants was examined over a 2-year study. many aspects of the plant/bacterial/insect interaction within the pitcher fluid are minimally understood because the bacterial taxa present in these pitchers have not been identified. thirteen isolates were characterized by 16s rdna sequencing and subsequent phylogenetic analysis. the proteobacteria were the most abundant taxa and included representatives from s ...200717380356
rice endophyte pantoea agglomerans ys19 forms multicellular symplasmata via cell aggregation.pantoea agglomerans is characterized by the formation of multicellular symplasmata. one unanswered question regarding this bacterium is how these structures are formed. in this study, the rice diazotrophic endophyte p. agglomerans ys19 was selected for exploration of this theme. ys19 was labeled with green fluorescent protein and the resulting recombinant ys19::gfp was observed to grow only slightly more slowly (a decrease of 5.5%) than the wild-type strain, and to show high gfp label stability ...200717391364
pantoea agglomerans liver abscess presenting with a painful thigh.this case highlights that an acute myositis leading to rhabdomyolysis may occur as a rare complication of hepatic abscess and shows the benefit of early recognition of this possible association. a 70-year-old man presented with fever and lower limb myalgia, with laboratory evidence of acute renal failure secondary to rhabdomyolysis. blood cultures revealed pantoea agglomerans, which led to identification of a hepatic abscess on computed tomography scan. supportive care together with antibiotics ...200717413296
[pantoea agglomerans--pathogen of elytrigia repens and arrhenatherum elatius diseases].strains of pantoea agglomerans were isolated from the samples with symptoms of bacterial damage of various organs of elytrigia repens and arrhenatherum elatius. the isolated bacteria are aggressive to different extent for a host-plant and a number of cultivated plants. some problems of evolution of this bacterial species parasitism are discussed.200717427410
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