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cytotoxic and multidrug resistance reversal activity of a vegetable, 'anastasia red', a variety of sweet pepper.the vegetable, anastasia red, capsicum annuum l. var. angulosum mill. (solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ods) column chromatography. the biological activities of extracts and fractions were determined. these extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (hsc-2, hsg) than against normal human gin ...200312722138
trpv1 activation and induction of nociceptive response by a non-pungent capsaicin-like compound, capsiate.capsiate is a capsaicin-like ingredient of a non-pungent cultivar of red pepper, ch-19 sweet. to elucidate the mechanisms underlying the non-pungency of capsiate, we investigated whether capsiate activates the cloned capsaicin receptor, trpv1 (vr1). in patch-clamp experiments, capsiate was found to activate trpv1 expressed transiently in hek293 cells with a similar potency as capsaicin. capsiate induced nociceptive responses in mice when injected subcutaneously into their hindpaws with a similar ...200312726827
short-term control of capsaicin on blood and oxidative stress of rats in vivo.capsaicin (8-methyl-n-vanillyl-6-nonenamide), a pungent component found in red pepper can induce body heat and possibly enhance blood flow as well as increase energy expenditure, and prevent oxidative stress. male wistar rats were divided into vehicle, 1 mg/kg body weight capsaicin and 3 mg/kg body weight capsaicin groups. samples were taken from the animals on day 1 of i.p. treatment with capsaicin and on 3 consecutive days of i.p. treatment with capsaicin. our investigation demonstrated that b ...200312748978
red pepper attenuates cholesteryl ester transfer protein activity and atherosclerosis in cholesterol-fed rabbits.the current study was conducted to examine the effect of red pepper supplementation on cholesteryl ester transfer protein (cetp) activity, along with its anti-atherosclerotic effect in cholesterol-fed rabbits.200312763278
safety assessment for genetically modified sweet pepper and tomato.the coat protein (cp) gene of cucumber mosaic virus (cmv) was cloned from a chinese cmv isolate, the camv promoter and nos terminator added and the gene construct was transformed into both sweet pepper and tomato plants to confer resistance to cmv. safety assessments of these genetically modified (gm) plants were conducted. it was found that these two gm products showed no genotoxicity either in vitro or in vivo by the micronucleus test, sperm aberration test and ames test. animal feeding studie ...200312767699
ferredoxin from sweet pepper (capsicum annuum l.) intensifying harpin(pss)-mediated hypersensitive response shows an enhanced production of active oxygen species (aos).the hypersensitive response (hr) is a form of cell death associated with plant resistance to pathogen infection. harpin(pss), an elicitor from the bacterium pseudomonas syringae pv. syringae, induces a hr in non-host plants. previously, we reported an amphipathic protein from sweet pepper interfering with harpin(pss)-mediated hr. in this report, we isolated and characterized a cdna clone encoded that amphipathic protein from sweet pepper. this protein is designated as pflp (plant ferredoxin-like ...200312777051
the sweet pepper ferredoxin-like protein (pflp) conferred resistance against soft rot disease in oncidium orchid.genetic engineering to date has not been used to introduce disease resistance genes into the orchid gene pool. the ferredoxin-like protein gene originally isolated from sweet pepper is thought to function as a natural defense against infection due to its antimicrobial properties. hence it was reasoned that introduction of this gene might produce oncidium plants resistant to erwinia carotovora, the causal agent for the soft rot disease. an expression vector containing sweet pepper ferredoxin-like ...200312779121
binary transformation systems based on 'shooter' mutants of agrobacterium tumefaciens: a simple, efficient and universal gene transfer technology that permits marker gene elimination.a simple transformation procedure with a positive selection scheme using the expression of the isopentenyl transferase ( ipt) gene of transfer dna (t-dna) 'shooter' mutants of agrobacterium tumefaciens was elaborated. after comparing several 'shooter' mutants we found that particular strains frequently produced phenotypically normal shoots after co-culturing with tobacco leaf explants. shoots selected for normal phenotype showed apical dominance and could be rooted with the same efficiency as no ...200312789522
migration and enrichment of arsenic in the rock-soil-crop plant system in areas covered with black shale, korea.the okchon black shale, which is part of the guryongsan formation or the changri formation of cambro-ordovician age in korea provides a typical example of natural geological materials enriched with potentially toxic elements such as u, v, mo, as, se, cd, and zn. in this study, the dukpyung and the chubu areas were selected to investigate the migration and enrichment of as and other toxic elements in soils and crop plants in areas covered with black shale. rock and soil samples digested in 4-acid ...200312806106
incorporation of carotenoids from paprika oleoresin into human chylomicrons.the intake of a carotenoid-rich diet is epidemiologically related to a lower risk for different chronic disorders like cardiovascular disease, some types of cancer or age-related macular degeneration. red pepper (capsicum annuum l.) and its dietary products contain a variety of carotenoids, which may contribute to the carotenoid pattern of human blood and tissues. the objective of the present study was to assess the availability of carotenoids from paprika oleoresin, including zeaxanthin, beta-c ...200312828795
involvement of reactive oxygen species in capsaicinoid-induced apoptosis in transformed cells.some varieties of sweet pepper accumulate non-pungent isosters of capsaicin, a type of compounds exemplified by capsiate. the only structural difference between capsaicin and capsiate is the link between the vanillyl and the acyl moieties, via an amide bond in the former and via an ester bond in the latter. by flow cytometry analyses we have determined that nor-dihydrocapsiate, a simplified analogue of capsiate, is a pro-oxidant compound that induces apoptosis in the jurkat tumor cell line. the ...200312868488
[preliminary protocol for systemic administration of capsaicin for the treatment of the burning mouth syndrome].burning mouth syndrome could be considered an expression of atypical facial pain. the recent neuropathological findings in bms may suggest the need for alternative therapies. this paper aims to evaluate if systemic capsaicin could be used for bms the- rapy.200312874531
a short-term nacl exposure increases the na(+) conductance of outward-rectified cation currents in the pith cells of sweet pepper.the regulatory role of pith cells in the stem in na(+) recirculation in sweet pepper was investigated by evaluating the transport characteristics of the plasma membrane of this cell type and comparison with those of root cells. ion conductivity and na(+) permeability of the plasma membranes of protoplasts of both cell types were studied with the patch-clamp technique in the whole-cell configuration, before and after addition of nacl to the bath medium. protoplasts of both pith and root cells sho ...200312893309
influences of gamma-irradiation and storage on the carotenoids of sun-dried and dehydrated paprika.the effects of drying methods, gamma-irradiation, and storage on the carotenoids (capsanthin, capsorubin, zeaxanthin, capsolutein, violaxanthin, beta-carotene, and beta-cryptoxanthin) of paprika were investigated. sun-dried and dehydrated paprika samples were irradiated in a (60)co gamma-irradiator at five doses (0, 2.5, 5.0, 7.5, and 10 kgy) in polyethylene bags and stored for 10 months at ambient temperature. individual carotenoid analyses were carried out on the paprika and fresh red pepper d ...200312903955
raman spectra of carotenoids in natural products.resonance raman spectra of naturally occurring carotenoids have been obtained from nautilus, periwinkle (littorina littorea) and clam shells under 514.5 nm excitation and these spectra are compared with the resonance raman spectra obtained in situ from tomatoes, carrots, red peppers and saffron. the tomatoes, carrots and red peppers gave rise to resonance raman spectra exhibiting a nu1 band at ca. 1520 cm(-1), in keeping with its assignment to carotenoids with ca. nine conjugated carbon-carbon d ...200312909134
upregulation of uncoupling proteins by oral administration of capsiate, a nonpungent capsaicin analog.capsiate is a nonpungent capsaicin analog, a recently identified principle of the nonpungent red pepper cultivar ch-19 sweet. in the present study, we report that 2-wk treatment of capsiate increased metabolic rate and promoted fat oxidation at rest, suggesting that capsiate may prevent obesity. to explain these effects, at least in part, we examined uncoupling proteins (ucps) and thyroid hormones. ucps and thyroid hormones play important roles in energy expenditure, the maintenance of body weig ...200312959953
origin, evolution, and metabolic role of a novel glycolytic gapdh enzyme recruited by land plant plastids.nad-specific glyceraldehyde-3-phosphate dehydrogenase (gapdh) is a cytosolic marker enzyme of eukaryotes (gapc; ec 1.2.1.12). land plants possess an additional nadp+-dependent enzyme (ec 1.2.1.13) within their chloroplasts which is composed of two subunits, gapa and gapb. another plastid gapdh enzyme (gapcp) was recently discovered in gymnosperms and ferns. this novel gapcp is closely related to cytosolic gapc and displays glycolytic nad+ cosubstrate specificity. here we show that this new gene ...200312962302
[eosinophilogenic and toxic action of red pepper in the guinea pig]. 195313116572
[red pepper, a too little used therapeutic agent for anorexia, liver congestion and various vascular disorders (hemorrhoids, varices)]. 201513413615
effect of red pepper and mustard infusions on the reflex phase of gastric secretion. 196114039394
[studies on the influence of diets and lipotropic substances upon the various organs and metabolic changes in rabbits on long term feeding with red pepper. i. histopathologic changes of the liver and spleen]. 196314083289
[studies on the influence of diets and lipotropic substances upon the various organs and metabolic changes in rabbits on long-term feeding with red pepper. ii. histopathologic changes of the various organs except the liver and spleen]. 196314086901
[spices, pimentos and mustards]. 196414268964
antioxidant systems and their relationship with the response of pepper fruits to storage at 20 degrees c.fresh peppers (capsicum annuum l., variety california) in their green and red ripe stages were stored at 20 degrees c for 7 and 19 days to determine the effects of storage on whole fruit antioxidant capacity (taa) and ascorbate (asc) content, as well as on some antioxidant enzyme activities, such as catalase (cat), superoxide dismutase (sod), and those of the asc-glutathione cycle. at least one mn-sod, two fe-sods, and three cuzn-sods were detected in the fruit extract after native polyacrylamid ...200314518958
calcium restores prepenetration morphogenesis abolished by polyamines in colletotrichum gloeosporioides infecting red pepper.colletotrichum gloeosporioides forms a specialized infection structure, an appressorium, to infect its host, red pepper. polyamines (putrescine, spermidine, and spermine) as well as s-adenosyl methionine inhibitor, methylglyoxal-bis-guanyl hydrazone (mgbg), impaired conidial germination and appressorium formation of c. gloeosporioides. curtailment of cell differentiation by polyamines and mgbg was more evident in conidial germination than in appressorium development. exogenous addition of calciu ...200314592714
peroxisomes from pepper fruits (capsicum annuum l.): purification, characterisation and antioxidant activity.pepper is a vegetable of importance in human nutrition. currently, one of the most interesting properties of natural products is their antioxidant content. in this work, the purification and characterisation of peroxisomes from fruits of a higher plant was carried out, and their antioxidative enzymatic and non-enzymatic content was investigated. green and red pepper fruits (capsicum annuum l., type lamuyo) were used in this study. the analysis by electron microscopy showed that peroxisomes from ...200314717445
atmospheric pressure photo-ionization liquid chromatography/mass spectrometric determination of aflatoxins in food.a comparison between electrospray ionization (esi) and the recent introduced atmospheric pressure photo-ionization (appi) technique is described for the liquid chromatography/mass spectrometric (lc/ms) determination of aflatoxin b1, b2, g1 and g2 in food. for the optimization of appi, several appi ion source parameters were examined. furthermore, the chemical noise and signal suppression of esi and appi by sample matrix interference were investigated. the results indicated that appi has the low ...200414744683
changes in the carotenoid metabolism of capsicum fruits during application of modelized slow drying process for paprika production.a temperature profile simulating the traditional slow drying process of red pepper fruits, which is conducted in la vera region (spain) for paprika production, was developed. carotenoid and ascorbic acid content, as well as moisture of fruits, were monitored during the slow drying process designed. data obtained suggested that the evolution of carotenoid concentration, the main quality trait for paprika, directly depend on the physical conditions imposed. during the drying process, three differe ...200414759142
effects of capsaicin on the sensorimotor function of the proximal stomach in humans.capsaicin-sensitive afferents have been implicated in the control of visceral sensorimotor functions.200414871281
inheritance of resistance to meloidogyne incognita race 2 in the hot pepper cultivar carolina cayenne (capsicum annuum l.).root-knot nematodes of the genus meloidogyne are important pathogens affecting vegetable crop production in brazil and worldwide. the pepper species capsicum annuum includes both hot and sweet peppers; very little emphasis has been placed on breeding sweet peppers for nematode resistance. we report on the inheritance of resistance to meloidogyne incognita (kofoid & white) chitwood race 2 in the hot pepper cultivar carolina cayenne. the hot pepper cv. carolina cayenne was used as seed parent and ...200214963835
[molecular aflp-marking of genotypes of pepper (capsicum annuum) cultivars].the results of aflp study of 14 capsicum annuum cultivars are presented. notwithstanding the known low genomic variation of large-fruited sweet pepper, aflp analysis proved to be suitable for detecting polymorphism and genotyping pepper cultivars. nine primer pairs were selected to allow identification of the cultivars under study. among-cultivar polymorphism detectable with these primers was estimated at 16.5%. a characteristic aflp pattern was obtained for each cultivar. several cultivar-speci ...200314964824
specific synthesis of 5,5'-dicapsaicin by cell suspension cultures of capsicum annuum var. annuum (chili jalapeño chigol) and their soluble and nacl-extracted cell wall protein fractions.hplc-uv, (1)h nmr, (13)c nmr, and (1)h-(1)h cosy analyses revealed that exogenous capsaicin was specifically converted into 5,5'-dicapsaicin by both cell suspension cultures of capsicum annuum var. annuum (chili jalapeño chigol) and their soluble and nacl-extracted cell wall protein fractions under oxidative conditions. in cell suspension cultures 5,5'-dicapsaicin was found only in biomass of capsaicin-fed cultures. this compound has not been detected before either in fresh fruits or in in vitro ...200414969559
effects of sesamin and capsaicin on the mrna expressions of delta6 and delta5 desaturases in rat primary cultured hepatocytes.sesamin, a lignan in sesame seeds and sesame seed oil, and capsaicin, the pungent principle of hot red pepper, affect lipid metabolism. sesamin specifically inhibits delta5 desaturase activity in the mortierella alpina fungus and rat liver microsomes, however, the effects of sesamin and capsaicin on mrna expressions of delta6 and delta5 desaturases are not still clear. in this study, we investigated the effects of sesamin and capsaicin on the desaturation indexes of delta6 [(gamma-linolenic acid ...200314974737
flavonoids in vegetable foods commonly consumed in brazil and estimated ingestion by the brazilian population.the objective of this work was to quantify the flavonoids present in foods most commonly consumed by the brazilian population. the predominant flavonoids found in largest abundance in all of the analyzed vegetables were glycosides of quercetin. in lettuce, a small amount of luteolin was also detected. in sweet pepper, quercetin and luteolin were both present. white onion [48-56 mg/100 g of fresh weight (fw), expressed as aglycon], red onion (40-100 mg/100 g of fw), red lettuce (67-67.2 mg/100 g ...200414995109
acaricidal activity of plant essential oils against dermanyssus gallinae (acari: dermanyssidae).the acaricial activity of 56 plant essential oils against poultry house-collected adult dermanyssus gallinae de geer was examined using direct contact and fumigation methods. in a filter paper contact bioassay, 100% mortality at 0.07 mgcm-2 was observed in bay, cade, cinnamon, clove bud, coriander, horseradish, lime dis 5f, mustard, pennyroyal, pimento berry, spearmint, thyme red and thyme white oils, whereas the mortality of these oils was significantly decreased at 0.02 mgcm-2. in fumigation t ...200415063940
qualitative and quantitative variation among volatile profiles induced by tetranychus urticae feeding on plants from various families.many plant species are known to emit herbivore-induced volatiles in response to herbivory. the spider mite tetranychus urticae koch is a generalist that can feed on several hundreds of host plant species. volatiles emitted by t. urticae-infested plants of 11 species were compared: soybean (glycine max), golden chain (laburnum anagyroides), black locust (robinia pseudo-acacia), cowpea (vigna unguiculata), tobacco (nicotiana tabacum), eggplant (solanum melalonga), thorn apple (datura stramonium), ...200415074658
growth promotion of red pepper plug seedlings and the production of gibberellins by bacillus cereus, bacillus macroides and bacillus pumilus.the growth of red pepper plug seedlings was promoted by bacillus cereus mj-1, b. macroides cj-29, and b. pumilus cj-69 isolated from the rhizosphere. gibberellins (gas), a well-known plant growth-promoting hormone, were detected in the culture broth of their rhizobacteria. among the gas, the contents of ga1, ga3, ga4, and ga7, physiologically active gas, were comparatively higher than those of others, suggesting that the growth promoting effect was originated from the gas. this isthe first repor ...200415127789
effect of aphid-infested plants on the host location and learning behaviour of the parasitoid aphelinus abdominalis.aphelinus abdominalis dalman (hymenoptera: aphelinidae) is a generalist parasitoid of cereal (e.g. sitobion avenae) and greenhouse aphids (e.g. macrosiphum euphorbiae). it differs from aphidius spp. (hymenoptera: braconidae) by its robust constitution, longevity and its habit to use aphids not only as hosts for its progeny but also as prey (host feeding). in addition, a. abdominalis does not evoke aphidius-characteristic defence responses by attacked aphids as this wasp approaches its hosts very ...200315149107
efficacy of salts against fungal diseases in glasshouse crops.the constant input of chemical fungicides against foliar diseases in glasshouse crops has instigated research on alternative control substances. various salts have been mentioned in the literature and we have tested several bicarbonates, sulphates and phosphates against powdery mildew fungi in cucumber, tomato, sweet pepper and rose and against bremia lactucae in lettuce. the experiments were run according to eppo guidelines for efficacy in glasshouses of applied plant research (ppo). plants wer ...200315151280
maximum tolerable dose of red pepper decreases fat intake independently of spicy sensation in the mouth.dietary red pepper suppresses energy intake and modifies macronutrient intake. we have investigated whether a stimulus in the mouth and the sensation of spiciness are necessary for red pepper-induced changes in energy and macronutrient intake in human volunteers. in a preliminary test, sixteen japanese male volunteers tasted samples of a soup with graded doses of red pepper in order to define a moderate and a maximum tolerable (strong) dose of red pepper. on the day of the experiment, a standard ...200415182402
dietary sources of vitamin c, vitamin e and specific carotenoids in spain.a cross-sectional study was conducted within the spanish cohort of the european prospective investigation in cancer and nutrition to assess the principal food sources of vitamin c, vitamin e, alpha-carotene, beta-carotene, lycopene, lutein, beta-cryptoxanthin and zeaxanthin in an adult spanish population. the study included 41446 healthy volunteers (25812 women and 15634 men), aged 29-69 years, from three spanish regions in the north (asturias, navarra and guipúzcoa) and two in the south (murcia ...200415182404
the aflatoxin contamination of some pepper types sold in turkey.in the present study, 44 red-scaled pepper and 26 red powder pepper samples obtained from various spice retailers in erzurum, and 20 isot (red pepper produced in sanliurfa) were investigated for aflatoxin contamination using thin layer chromatography. aflatoxin (b + g) was found in 8 red pepper samples (18.2%), and 3 red powder peppers (10.7%), and 1 isot sample (5%). the aflatoxin amount ranged from 1.1 to 97.5 ppb in all samples investigated. the highest amount of aflatoxin was found in red-sc ...200415183993
characterization and quantitation of antioxidant constituents of sweet pepper (capsicum annuum l.).sweet peppers (capsicum annuum l.) cv. vergasa have been studied at four maturity stages (immature green, green, immature red, and red). the individual phenolics (hydroxycinnamic acids and flavonoids), vitamin c (ascorbic acid and dehydroascorbic acid), and individual carotenoids were characterized and quantified. five hydroxycinnamic derivatives and 23 flavonoids were characterized and quantified from the pericarp of sweet pepper by high-performance liquid chromatography-diode array detection-e ...200415186108
involvement of nadph oxidase-mediated generation of reactive oxygen species in the apototic cell death by capsaicin in hepg2 human hepatoma cells.although capsaicin (8-methyl-n-vanillyl-6-nonenamide), a pungent ingredient in a variety of red peppers of the genus capsicum, has been shown to induce apoptotic cell death in many cancer cells, the exact mechanism of this action of capsaicin is not completely understood. in this study, we investigated the possible mediation of the nadph oxidase-modulated production of reactive oxygen species (ros) in the apoptotic mechanism of capsaicin in hepg2 human hepatoblastoma cells. capsaicin induced apo ...200415190937
content of toxic and essential metals in canned mussels commonly consumed in tenerife, canary islands, spain.concentrations of three toxic heavy metals (hg, pb, cd) and six essential heavy metals (fe, zn, mn, cr, cu, ni) were determined in mussel conserves (mytilus galloprovincialis, bivalvia, mollusca) consumed habitually by individuals in tenerife (canary islands, spain). a total of 600 samples were analyzed, corresponding to six different commercial brands and four different processing types: pickled sauce (mixture of olive oil, vinegar, red pepper, laurel, and salt), coquille st. jacques sauce (coq ...200415270515
flower and fruit abortion in sweet pepper in relation to source and sink strength.source strength (assimilate supply) and sink strength (assimilate demand) of the plant were varied in different ways to investigate to what extent flower/fruit abortion in sweet pepper (capsicum annuum l.) is determined by the availability of assimilates. source strength was varied by changing the light level, plant density, and leaf pruning. sink strength was varied by changing the temperature and the number and position of earlier formed fruits. shading as well as heating for short periods sho ...200415333643
effect of red pepper capsicum annuum var. conoides and garlic allium sativum on plasma lipid levels and cecal microflora in mice fed beef tallow.antihyperlipidemia or hypocholesterolaemic and antibacterial activities of red hot pepper and garlic are well known. to determine the effect of the dietary spices ingested to suppress blood lipids on the intestinal condition, we examined plasma lipid levels and cecal microflora in mice that were fed diets containing 19% (w/w) beef tallow and 2% red pepper capsicum annuum var. conoides 'takanotume' (rp) or garlic allium sativum 'white' (gp) for 4-weeks. plasma triacylglyceride level was suppresse ...200415354321
liquid chromatographic determination of novel aminithiazolecarboxamide fungicide residues in soil and crops using online solid-phase extraction.a simple and convenient liquid chromatographic method has been developed and applied to the analysis of the novel aminothiazolecarboxamide fungicide, ethaboxam, in soil and crops. after the isolation and concentration of analyte from soil and crops, clean-up and separation of sample solutions are performed by high-performance liquid chromatography with online solid-phase extraction. good linearity (r2 > 0.9995), recovery [for soil, 95.3-98.4%, and crops (grape, red pepper), 92.9-95.9%], and repe ...200415355576
antimicrobial and antioxidant effects of milk protein-based film containing essential oils for the preservation of whole beef muscle.milk protein-based edible films containing 1.0% (w/v) oregano, 1.0% (w/v) pimento, or 1.0% oregano-pimento (1:1) essential oils mix were applied on beef muscle slices to control the growth of pathogenic bacteria and increase the shelf life during storage at 4 degrees c. meat and film were periodically tested during 7 days for microbial and biochemical analysis. the lipid oxidation potential of meat was evaluated by the determination of thiobarbituric reactive substances (tbars). the availability ...200415373399
method validation of resistive heating--gas chromatography with flame photometric detection for the rapid screening of organophosphorus pesticides in fruit and vegetables.a rapid method for the screening of organophosphorus (op) pesticides in fruit and vegetables is reported. sample extracts were analysed using resistive heating-gas chromatography (rh-gc) with flame photometric detection (fpd). a carbofrit insert in the gc liner allowed injection of crude extracts onto the gc system. separation of up to 20 pesticides was achieved in 4.3 min with excellent retention time stability. signal-to-noise ratios of 5:1 or better were obtained for the majority of the pesti ...200415387192
quantitative inheritance of fruit size in red pepper (capsium frutescens l.). 195015414933
capsaicin-induced apoptosis of h-ras-transformed human breast epithelial cells is rac-dependent via ros generation.many studies have focused on the anticarcinogenic, antimutagenic or chemopreventive activities of capsaicin (trans-8-methyl-n-vanillyl-6-nonenamide) which is a major pungent ingredient in red pepper. we have previously shown that capsaicin selectively induces apoptosis in h-ras-transformed mcf10a human breast epithelial cells but not in their normal cell counterparts (int. j. cancer, 103, 475-482, 2003). in this study, we investigated the possible roles of reactive oxygen species (ros) and rac1 ...200415460446
trpv1 and the gut: from a tasty receptor for a painful vanilloid to a key player in hyperalgesia.capsaicin, the pungent ingredient in red pepper, has been used since ancient times as a spice, despite the burning sensation associated with its intake. more than 50 years ago, nikolaus jancso discovered that capsaicin can selectively stimulate nociceptive primary afferent neurons. the ensuing research established that the neuropharmacological properties of capsaicin are due to its activation of the transient receptor potential ion channel of the vanilloid type 1 (trpv1). expressed by primary af ...200415464036
residues and half-lives of pyrethrins on field-grown pepper and tomato.the dried flower heads of tanacetum cinerariifolium trev. (family: compositae) contain insecticidal compounds collectively called "pyrethrins." pyrethrins are the subject of intense interest for use in crop protection because their toxicological properties permit control of certain insect species at application rates as low as 5-10 g ai acre(-1). seedlings of sweet pepper, capsicum annuum l. cv. bell boy hybrid and tomato, lycopersicon esculentum mill. cv. mountain spring f1 hybrid were planted ...200415473632
renal lesions in streptozotocin-induced diabetic rats maintained on onion and capsaicin containing diets.onion (allium cepa) powder and capsaicin, the pungent principle of red pepper (capsicum annum) were added in the amounts of 3 g% and 15 mg%, respectively, to the diet of streptozotocin-induced diabetic wistar rats for 8 weeks. the presence of renal lesions was assessed by the extent and quality of proteinuria and by the leaching of renal tubular enzymes into the urine. renal integrity was assessed by measuring the activities in the kidney tissue of several key enzymes of carbohydrate metabolism ...199915539326
pharmacological effects of fermented red pepper.the pharmacological effects were investigated of fermented red pepper (hf-s), which consisted of 14.7% carbohydrate, 1.5% lipid, 4.9% protein, 0.3% ash, 78.2% moisture content, with 0.15% capsaicin and 0.06% dihydrocapsaicin. oral administration of 0.25 ml hf-s for 3 weeks produced significant changes of the perirenal fat pad weight compared with the hf-control group, suggesting a suppressive effect on lipid accumulation and a significant decrease in the risk of arteriosclerosis. the hf-s (0.25 ...200415597334
sensory and gastrointestinal satiety effects of capsaicin on food intake.decreased appetite and increased energy expenditure after oral consumption of red pepper has been shown.200515611784
suppression of the nuclear factor-kappab activation pathway by spice-derived phytochemicals: reasoning for seasoning.the activation of nuclear transcription factor kappab has now been linked with a variety of inflammatory diseases, including cancer, atherosclerosis, myocardial infarction, diabetes, allergy, asthma, arthritis, crohn's disease, multiple sclerosis, alzheimer's disease, osteoporosis, psoriasis, septic shock, and aids. extensive research in the last few years has shown that the pathway that activates this transcription factor can be interrupted by phytochemicals derived from spices such as turmeric ...200415659827
production of an anti-fungal substance for biological control of phytophthora capsici causing phytophthora blight in red-peppers by streptomyces halstedii.the culture broth of streptomyces halstedii aj-7 suppressed the growth of phytophthora capsici, which causes phytophthora blight in red-peppers, with less than 1% survival of the pathogen after 12 h of treatment. the low molecular fraction (< or = 10 kda) of the culture broth retained anti-fungal activity against p. capsici after being held at 100 degrees c for 6 h.200515717130
residual behaviour of profenofos on some field-grown vegetables and its removal using various washing solutions and household processing.profenofos (selecron 72% ec), was sprayed on field-grown pepper and eggplant at the recommended rate of 1.28 kg a,i/ha. fruit samples were collected at 1 h to 14 days after application and analysed to determine the content and dissipation rate of profenofos. the effect of different washing solutions and some household processing on the removal of such residues from treated vegetables were also investigated. profenofos residues were quantified by using gas chromatography. the results showed that ...200515721202
antioxidant activity of the main phenolic compounds isolated from hot pepper fruit (capsicum annuum l).four cultivars (bronowicka ostra, cyklon, tornado, and tajfun) of pepper fruit capsicum annuum l. were studied for phenolics contents and antioxidant activity. two fractions of phenolics, flavonoids (with phenolic acids) and capsaicinoids, were isolated from the pericarp of pepper fruit at two growth stages (green and red) and were studied for their antioxidant capacity. both fractions from red fruits had higher activities than those from green fruits. a comparison of the capsaicinoid fraction w ...200515740069
determination of the equilibrium, kinetic and thermodynamic parameters of adsorption of copper(ii) ions onto seeds of capsicum annuum.adsorption of copper ions onto capsicum annuum (red pepper) seeds was investigated with the variation in the parameters of ph, contact time, adsorbent and copper(ii) concentrations and temperature. the nature of the possible adsorbent and metal ion interactions was examined by the ftir technique. the copper(ii) adsorption equilibrium was attained within 60 min. adsorption of copper(ii) ions onto c. annuum seeds followed by the langmuir, freundlich and dubinin-radushkevich (d-r) isotherm models. ...200515990228
safety of capsaicin cough challenge testing.capsaicin, the pungent extract of red peppers, has achieved widespread use in clinical research because it induces cough in a dose-dependent and reproducible manner. although > 2 decades of experience has led investigators to consider capsaicin cough challenge testing a safe diagnostic modality, this issue has not been specifically addressed in the literature.200516002935
effects of flavorings, storage conditions, and storage time on survival of staphylococcus aureus in sürk cheese.the objectives of this study were to determine the cumulative effects of flavorings (chili pepper, thyme, mint, cumin, nutmeg, allspice, clove, cinnamon, black pepper, salt, and hot red pepper paste), storage conditions, and storage time on the survival of staphylococcus aureus in sürk cheese and to monitor the associated chemical changes. sürk cheese, a traditional turkish cheese, was produced by heating diluted nonfat yogurt and adding flavorings to the resultant acid-heat curd. the cheese was ...200516013393
the effects of ripening stage and processing systems on vitamin c content in sweet peppers (capsicum annuum l.).morrón pepper of 'fresno de la vega' (capsicum annuum l.) is a big sweet variety cultivated in the province of león (northwestern spain). changes in vitamin c content of this variety of pepper as a function of ripeness, storage and different preservation systems were studied. the ascorbic acid content increases in peppers as they ripen. for green mature, breaker and red peppers values of 107.3+/-1.84, 129.6+/-3.11 and 154.3+/-7.56 mg/100 g edible portion were found. the vitamin c content for gre ...200516019314
effect of temperature and host plant leaf morphology on the efficacy of two entomopathogenic biocontrol agents of thrips palmi (thysanoptera: thripidae).the efficacy of two entomopathogenic biocontrol agents, steinernema feltiae (filipjev) and verticillium lecanii (zimmerman) viégas (reclassified now as lecanicillium muscarium (petch) zare & gams), against thrips palmi karny was investigated. assessments of the effect of temperature on the efficacy of s. feltiae indicated that higher mortality of t. palmi was recorded at 20 degrees c compared to either 15 or 25 degrees c, whereas significantly higher t. palmi mortality followed application of l. ...200516048680
inhibitory effects of glycolipids fraction from spinach on mammalian dna polymerase activity and human cancer cell proliferation.we succeeded in purifying the fraction containing the major glycolipids in monogalactosyl diacylglycerol, digalactosyl diacylglycerol and sulfoquinovosyl diacylglycerol (sqdg) from dried vegetables. this glycolipids fraction was an inhibitor of dna polymerase alpha (pol alpha) in vitro and also the proliferation of human cancer cells. in this study, eight common vegetables were investigated in terms of the glycolipids fraction, the amounts of major glycolipids, mammalian dna polymerase inhibitor ...200516081275
[cdna cloning and expression of a cytosolic small heat shock protein gene (cahsp18) from capsicum annuum].full length 779 bp cytosolic clsss i shsp cdna was cloned from heat-shocked sweet pepper leaves using a pcr approach with degenerate primers designed from conserved motifs found in a number of plant cytosolic clsss i shsp genes, named cahsp18. its accession number is ay284925. cahsp18 was high identified with nicotiana tabacum (fig.1). a multi-gene family was found in sweet pepper genomic dna by southern-blot analysis (fig.3). northern blot revealed that the expression of cahsp18 in sweet pepper ...200516121013
characterization of carotenoids and carotenoid esters in red pepper pods (capsicum annuum l.) by high-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry.carotenoids and carotenoid esters were extracted from red pepper pods (capsicum annuum l.) without saponification. among the 42 compounds detected, 4 non-esterified, 11 mono- and 17 diesters were characterized based on their retention times, uv/vis spectra and their fragmentation patterns in collision-induced dissociation experiments in atmospheric pressure chemical ionization mass spectrometry (apci-ms). positive and negative ion mode measurements were used for the characterization of major and ...200516124038
gene stacking in phalaenopsis orchid enhances dual tolerance to pathogen attack.cymbidium mosaic virus (cymmv) and erwinia carotovora have been reported to cause severe damage to orchid plants. to enhance the resistance of orchids to both viral and bacterial phytopathogens, gene stacking was applied on phalaenopsis orchid by double transformation. plbs originally transformed with cymmv coat protein cdna (cp) were then re-transformed with sweet pepper ferredoxin-like protein cdna (pflp) by agrobacterium tumefaciens, to enable expression of dual (viral and bacterial) disease ...200516145836
bioactivities of anastasia black (russian sweet pepper).anastasia black (russian sweet pepper) of capsicum annuum l. var. angulosum mill. (solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of twenty-three fractions by silica gel or octadecylsilane (ods; c18) column chromatography. these extracts and fractions were investigated for their cytotoxicity, anti-human immunodeficiency virus (hiv), anti-helicobacter pylori (h. pylori), urease inhibition and multidrug r ...200516158935
activity of some aminoglycoside antibiotics against true fungi, phytophthora and pythium species.to investigate the in vitro antifungal and antioomycete activities of some aminoglycosides against true fungi and phytophthora and pythium species and to evaluate the potential of the antibiotics against phytophthora late blight on plants.200516162234
aflatoxin and ochratoxin a content of spices in hungary.in october and november 2004, 91 spice samples (70 ground red pepper, six black pepper, five white pepper, five spice mix and five chilli samples), the majority of which originated from commercial outlets, were analysed for aflatoxins b1, b2, g1 and g2 (afb1, afb2, afg1, afg2) and ochratoxin a (ota) content by high-performance liquid chromatography (hplc) after immunoaffinity column clean-up. eighteen of the 70 ground red pepper samples contained afb1, seven of them in a concentration exceeding ...200516192072
simultaneous determination of sudan dyes and carotenoids in red pepper and tomato products by hplc.to simultaneously detect sudan dyes and carotenoids in red pepper and tomato products, high-performance liquid chromatography (hplc) methods with photodiode-array detection are developed and validated. the methods include the use of end-capped and nonend-capped adsorbents with a gradient elution system starting with water containing methanol. water content of 9% in the starting mobile phase is found to be necessary to ensure sufficient separation of sudan dyes and to avoid overlapping or interfe ...200516212791
identification of a male-produced aggregation pheromone in the western flower thrips frankliniella occidentalis.two major components have been detected in the headspace volatiles of adult male frankliniella occidentalis (pergande) (thysanoptera: thripidae) that are not present in the headspace volatiles of adult females. the compounds were identified as (r)-lavandulyl acetate and neryl (s)-2-methylbutanoate by comparison with synthetic standards using gas chromatography (gc), gc mass spectrometry (ms), and chiral gc. field trials were conducted with synthetic compounds in naturally infested crops of sweet ...200516222777
ovipositional deterrent in the sweet pepper, capsicum annuum, at the mature stage against liriomyza trifolii (burgess).liriomyza trifolii (burgess), the american serpentine leafminer fly, is well known as a serious pest throughout the world. this insect attack over 21 different plant families including solanaceae plants. the mature sweet pepper, capsicum annuum (solanaceae), however, shows resistance to this leafminer fly. this resistance is based on the ovipositional deterrent in the sweet pepper leaf against the fly species. based on bioassay-guided fractionation, luteolin 7-o-beta-d-apiofuranosyl-(1-->2)-beta ...200516244431
apoptosis induction by dohevanil, a dha substitutive analog of capsaicin, in mcf-7 cells.capsaicin (8-methyl-n-vanillyl-6-nonenamide), a major pungent ingredient in a variety of red peppers of the genus capsicum, is a type of vanilloid. it has been shown to induce apoptosis in many cell types. the effects of vanilloids on apoptosis induction are thought to be correlated with the length and degree of the unsaturation of the fatty acyl chains. in this study, we compared the effect of capsaicin and its docosahexaenoic acid (dha, c22:6) analog (we named as dohevanil) on human breast can ...200616260002
expression of the hypersensitive response-assisting protein in arabidopsis results in harpin-dependent hypersensitive cell death in response to erwinia carotovora.active defense mechanisms of plants against pathogens often include a rapid plant cell death known as the hypersensitive cell death (hcd). hypersensitive response-assisting protein (hrap) isolated from sweet pepper intensifies the harpin(pss)-mediated hcd. here we demonstrate that constitutive expression of the hrap gene in arabidopsis results in an enhanced disease resistance towards soft rot pathogen, e. carotovora subsp. carotovora. this resistance was due to the induction of hcd since differ ...200516270229
effect of a mixture of red pepper (capsicum frutescens) and an amino-acid on gastric acid secretion.the effects of a mixture of red pepper suspension and alpha-alanine (an amino-acid) on gastric acid secretion in 33 duodenal ulcer and 18 non-duodenal ulcer patients were studied. this mixture caused an increase of gastric acid in the two groups of patients, more so in the duodenal ulcer group (0.025 > p > 0.010). compared with the results of previous studies the administration of alpha-alanine in red pepper suspension produced less gastric acid than the fresh red pepper suspension alone.197616295058
ovipositional deterrent on mature stage of sweet pepper, capsicum annuum, against liriomyza trifolii (burgess).liriomyza trifolii (burgess), the american serpentine leafminer fly, is a well-known serious pest in the world. this insect species attacks plants of more than 21 families including solanaceae plants. a sweet pepper, capsicum annuum (solanaceae), on mature stage, however, shows resistance to this leafminer fly. this resistance is based on the ovipositional deterrent in the sweet pepper leaf against the fly species. based on the bioassay guided fractionation, phytol [(2e)-3,7,11,15-tetramethyl-2- ...200916320617
impact of food disinfection on beneficial biothiol contents in vegetables.in this work we investigated the impact of food disinfection on the beneficial biothiol contents in a suite of vegetables consumed daily, including spinach, green bean, asparagus, cucumber, and red pepper. four disinfection technologies commonly studied and/or used in food processing and preservation, including hydrogen peroxide, free chlorine, and gaseous- and aqueous-phase ozone, were examined with common dosages and contact times. results indicate that the common disinfection technologies may ...200516332139
gibberellins-producing rhizobacteria increase endogenous gibberellins content and promote growth of red peppers.the growth of red pepper plants was enhanced by treatment with the rhizobacterium, bacillus cereus mj-1. red pepper shoots showed a 1.38-fold increase in fresh weight (fw) and roots showed a 1.28-fold fw gain. this plant growth-promoting rhizobacterium (pgpr) has been reported to produce gibberellins (gas). other gas-producing rhizobacteria, bacillus macroides cj-29 and bacillus pumilus cj-69, also enhanced the fw of the plants. they were less effective than b. cereus mj-1, though. the endogenou ...200516410767
capsaicin-induced cell death in a human gastric adenocarcinoma cell line.capsaicin, a pungent ingredient found in red pepper, has long been used in spices, food additives, and drugs. cell death induced by the binding of capsaicin was examined in a human gastric adenocarcinoma cell line (ags cells).200516419151
hydrolysis of zeaxanthin esters by carboxyl ester lipase during digestion facilitates micellarization and uptake of the xanthophyll by caco-2 human intestinal cells.zeaxanthin (zea) and lutein are the only dietary carotenoids that accumulate in the macular region of the retina and lens. it was proposed that these carotenoids protect these tissues against photooxidative damage. few plant foods are enriched in zea, and information about the bioavailability of zea from these foods and its accumulation in ocular tissues is limited. the amounts of free zea and its mono- and diesters were measured for several plant foods that have relatively high concentrations o ...200616484529
overexpression of cahsp26 in transgenic tobacco alleviates photoinhibition of psii and psi during chilling stress under low irradiance.a sweet pepper cdna clone, cahsp26 encoding the chloroplast (cp)-localized small heat shock protein (shsp), was isolated and characterized with regard to its sequence, response to various temperatures and function in transgenic tobacco plants. the deduced amino acid sequence contained three highly conserved regions, showing high identities with other plant shsps. expression of the cahsp26 gene showed that the mrna accumulation of cahsp26 was induced by heat stress. higher transcript levels were ...200716513207
capsaicin, a component of red peppers, inhibits the growth of androgen-independent, p53 mutant prostate cancer cells.capsaicin is the major pungent ingredient in red peppers. here, we report that it has a profound antiproliferative effect on prostate cancer cells, inducing the apoptosis of both androgen receptor (ar)-positive (lncap) and -negative (pc-3, du-145) prostate cancer cell lines associated with an increase of p53, p21, and bax. capsaicin down-regulated the expression of not only prostate-specific antigen (psa) but also ar. promoter assays showed that capsaicin inhibited the ability of dihydrotestoste ...200616540674
dehydro-alpha-lapachone isolated from catalpa ovata stems: activity against plant pathogenic fungi.the methanol extract of stems of catalpa ovata g don exhibits potent in vivo antifungal activity against magnaporthe grisea (hebert) barr (rice blast) on rice plants, botrytis cinerea pers ex fr (tomato grey mould) and phytophthora infestans (mont) de bary (tomato late blight) on tomato plants, puccinia recondita rob ex desm (wheat leaf rust) on wheat plants and blumeria graminis (dc) speer f. sp. hordei marchal (barley powdery mildew) on barley plants. an antifungal substance was isolated and i ...200616550502
organic acids, sugars, and l-tryptophane in exudates of vegetables growing on stonewool and their effects on activities of rhizosphere bacteria.the influence of stonewool substrate on the exudation of the major soluble carbon nutrients and of the auxin precursor tryptophane for pseudomonas biocontrol agents was studied. to this end, the composition of the organic acids and sugars, as well that of tryptophane, of axenically collected exudates of seed, seedlings, and roots of tomato, cucumber, and sweet pepper was determined. the major results were as follows. i) the total amount of organic acid is much higher than that of total sugar. ii ...200616570655
antiobesity effect of kochujang (korean fermented red pepper paste) extract in 3t3-l1 adipocytes.kochujang (korean fermented red pepper paste) is a mixture of fermented soybeans, wheat, and red pepper powder. kochujang has been reported to reduce body fat gain and lipid levels of adipose tissues and serum in rats. we studied the inhibitory effect of kochujang on lipid accumulation and investigated the molecular mechanism of the action in 3t3-l1 adipocytes by measuring the expression levels of adipocyte-specific genes by real-time reverse transcription-polymerase chain reaction. when 3t3-l1 ...200616579723
the effect of cooking method upon the titratable acidity of a popular vegetarian dish--scope for reducing its erosive potential?the purpose of this in vitro study was to determine the effect of cooking method on the erosive potential of ratatouille. two cooking methods, stewing and oven roasting, were applied to standardised ingredients taken from the same fruits and vegetables. the resultant dishes were liquidised and diluted with 100 mls of distilled water. five 25 ml samples of each group were titrated to ph 7.0 against 0.1 molar sodium hydroxide. in order to ascertain the relative contribution of each ingredient each ...200616599095
capsaicin and gastric ulcers.in recent years, infection of the stomach with the organism helicobacter pylori has been found to be the main cause of gastric ulcers, one of the common ailments afflicting humans. excessive acid secretion in the stomach, reduction in gastric mucosal blood flow, constant intake of non-steroid anti-inflammatory drugs (nsaids), ethanol, smoking, stress etc. are also considered responsible for ulcer formation. the prevalent notion among sections of population in this country and perhaps in others i ...200616621751
effect of different thai traditional processing of various hot chili peppers on urethane-induced somatic mutation and recombination in drosophila melanogaster: assessment of the role of glutathione transferase activity.four different thai traditional chili peppers, namely bird pepper (capsicum frutescens), red chili spur peppers (capsicum annuum), green bell peppers and sweet pepper (c. annuum) were investigated for their antimutagenic properties. each chili was prepared in three formulations commonly used for chili food processing; raw paste (chili ground in water), pickled in vinegar or stir-fried in palm oil. each sample was tested for its antimutagenic effect against urethane by using the somatic mutation ...200616624474
function of rhizodermal transfer cells in the fe stress response mechanism of capsicum annuum l.a variety of red pepper (capsicum annuum l., cv yaglik) responds to fe deficiency stress with simultaneously enhanced h(+) extrusion, reduction of ferric ions and synthesis of malic and citric acid in a swollen subapical root zone densely covered with root hairs. it is demonstrated that these stress responses temporally coincide with the development of rhizodermal and hypodermal transfer cells in this root zone. during stress response the transfer cells show a marked autofluorescence which could ...198616665060
regulation of carbon partitioning in source and sink leaf parts in sweet pepper (capsicum annuum l.) plants : role of fructose 2,6-bisphosphate.area expansion rate, partitioning of photosynthetically fixed carbon, and levels of fructose 2,6-bisphosphate (fru-2,6-p(2)) were determined in individual parts of developing leaves of sweet pepper (capsicum annuum l.). the base was rapidly expanding and allocated less carbon to sucrose synthesis in comparison to the leaf tip, where expansion had almost stopped. the change in leaf expansion rate and carbon partitioning happened gradually. during day time levels of fru-2,6-p(2) were consistently ...199016667515
short-term vegetable intake by young children classified by 6-n-propylthoiuracil bitter-taste phenotype.genetic variation in sensitivity to the bitterness of 6-n-propylthiouracil (prop) is thought to play a role in the acceptance and rejection of bitter-tasting vegetables by young children.200616825702
[an analysis of sweet pepper seeds with space flight mutagenesis by ftir].the first-time application of ftir to analyzing and comparing two sweet pepper seeds lines of space mutation and two sweet pepper seeds lines bred in system approaches as well as their spectra is presented. the ir spectrum of sweet pepper seeds lines is mainly composed of the band of adsorption of protein and that of carbohydrates. the absorbance of the two sweet pepper seeds lines of space mutation at 2854, 1652, 1542 and 1160-1062 cm(-1) was enhanced. the peak near 2854 cm(-1) is attributable ...200616830749
identification of a novel cys2/his2-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco.in a previous work, we identified a cys(2)/his(2)-type zinc-finger transcription repressor, (zft1), that functions in a spermine-mediated signal transduction pathway in tobacco plants. database search disclosed the presence of another cys(2)/his(2)-type zinc-finger protein zfp1 (accession number aac06243) in tobacco plants. in this work, we characterized zfp1 and investigated whether this protein is also involved in a spm-signaling pathway. this factor showed the highest identity to petunia zpt2 ...200716882456
effect of an acute intraluminal administration of capsaicin on oesophageal motor pattern in gord patients with ineffective oesophageal motility.ineffective oesophageal motility (iom) is a functional disorder affecting about 50% of gastro-oesophageal reflux disease (gord) patients. this disease in a severe form limits the clearing ability of the oesophagus and is considered one of the predictive factors for poorer gord resolution. capsaicin, the active compound of red pepper, exerts a prokinetic effect on oesophageal motility in healthy subjects by increasing the amplitude of body waves, even if no evidence exists on its possible role in ...200616918727
capsaicinol: synthesis by allylic oxidation and its effect on trpv1-expressing cells and adrenaline secretion in rats.capsaicinol is an ingredient of hot red pepper. in this study, we developed a novel method for capsaicinol synthesis and examined capsaicinol's physiological effects on capsaicin receptor (trpv1)-related actions. allylic oxidation of capsaicin by palladium acetate (pd(oac)(2)) resulted in the formation of (+/-)-capsaicinol acetate at a 7.2% yield in a single step. the effectiveness of (+/-)-capsaicinol in trpv1 activation (ec(50)=1.1 microm) was found to be weaker than that of capsaicin (ec(50)= ...200616926503
capsaicin induced cell cycle arrest and apoptosis in human esophagus epidermoid carcinoma ce 81t/vgh cells through the elevation of intracellular reactive oxygen species and ca2+ productions and caspase-3 activation.capsaicin (n-vanillyl-8-methyl-1-nonenamide) is found in pungent fruits, especially in red pepper. many studies have focused on the anticarcinogenic, antimutagenic or chemopreventive activities of capsaicin. however, the effects of capsaicin on human esophagus epidermoid carcinoma cells have never been investigated. in this study, we investigated the effects of capsaicin on esophagus epidermoid carcinoma cells in vitro and further examined the molecular mechanisms of capsaicin-induced apoptosis ...200616942782
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