Publications

contributions of the response regulators ssk1p and skn7p in the pseudohyphal development, stress adaptation, and drug sensitivity of the opportunistic yeast candida lusitaniae.	we recently characterized the histidine kinase receptor genes of candida lusitaniae. for the present study, we have further investigated the role of ssk1 and skn7, encoding response regulators. the results of functional analysis of mutants indicated that ssk1p is involved in osmotolerance and pseudohyphal development, whereas skn7p appears crucial for oxidative stress adaptation.	2008	18408054
identification of yeast and bacteria involved in the mezcal fermentation of agave salmiana.	aims: to identify the yeast and bacteria present in the mezcal fermentation from agave salmiana. methods and results: the restriction and sequence analysis of the amplified region, between 18s and 28s rdna and 16s rdna genes, were used for the identification of yeast and bacteria, respectively. eleven different micro-organisms were identified in the mezcal fermentation. three of them were the following yeast: clavispora lusitaniae, pichia fermentans and kluyveromyces marxianus. the bacteria foun ...	2008	18489025
characterization of cellobiose fermentations to ethanol by yeasts.	twenty-two different yeasts were screened for their ability to ferment both glucose and cellobiose. the fermentation characteristics of candida lusitaniae (nrrl y-5394) and c. wickerhamii (nrrl y-2563) were selected for further study because their initial rate of ethanol production from cellobiose was faster than the other test cultures. c. lusitaniae produced 44 g/l ethanol from 90 g/l cellobiose after 5-7 days. when higher carbohydrate concentrations were employed, fermentation ceased when the ...	1983	18548669
[the fungicidal activity and paradoxical effect of caspofungin against yeast. influence of culture medium and incubation time].	the effect of culture medium and incubation time on the fungicidal activity of caspofungin and on the frequency of paradoxical effect has been studied in 144 blood culture isolates (42, candida tropicalis, 32 candida glabrata, 21 candida krusei, 20 candida parapsilosis, 11 candida guilliermondii, 4 candida famata, 3 candida lusitaniae, 3 blastoschizomyces capitatus, 2 saccharomyces cerevisiae, 2 yarrowia lipolytica, 1 candida inconspicua, 1 candida lambica, 1 candida sake y 1 pichia ohmeri). the ...	2007	18563217
evidence of recent interkingdom horizontal gene transfer between bacteria and candida parapsilosis.	to date very few incidences of interdomain gene transfer into fungi have been identified. here, we used the emerging genome sequences of candida albicans wo-1, candida tropicalis, candida parapsilosis, clavispora lusitaniae, pichia guilliermondii, and lodderomyces elongisporus to identify recent interdomain hgt events. we refer to these as ctg species because they translate the ctg codon as serine rather than leucine, and share a recent common ancestor.	2008	18577206
identification of yeasts isolated from commercial shell eggs stored at refrigerated temperatures.	yeasts and molds can grow on or in eggs, causing spoilage. washed and unwashed eggs (treatments) were collected aseptically on three separate days (replications) from a commercial processing facility and stored for 10 weeks at 4 degrees c. ten eggs from each treatment were sampled weekly (110 eggs per treatment per replication). yeasts and molds were enumerated from external shell rinses by plating onto acidified potato dextrose agar. yeast colonies were picked randomly and stored for subsequent ...	2008	18592756
debaryomyces hansenii (candida famata), a rare human fungal pathogen often misidentified as pichia guilliermondii (candida guilliermondii).	debaryomyces hansenii is a hemiascomycetous yeast commonly found in natural substrates and in various types of cheese. pichia guilliermondii is widely distributed in nature and is a common constituent of the normal human microflora. both species have been described in human infections but are extremely difficult to differentiate phenotypically. thus, frequent errors in identification occur. the 62 clinical and environmental isolates sent between 2000 and 2007 to the french national reference cen ...	2008	18701668
microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge.	the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ...	2008	18838186
insight into the role of hog pathway components ssk2p, pbs2p, and hog1p in the opportunistic yeast candida lusitaniae.	in the present study, we have investigated the role of ssk2, pbs2, and hog1, encoding modules of the high-osmolarity-glycerol mitogen-activated protein kinase pathway in candida lusitaniae. functional analysis of mutants indicated that ssk2p, pbs2p, and hog1p are involved in osmotolerance, drug sensitivity, and heavy metal tolerance but not in oxidant stress adaptation.	2008	18952902
[in vitro amphotericin b susceptibility of korean bloodstream yeast isolates assessed by the clsi broth microdilution method, etest, and minimum fungicidal concentration test].	although amphotericin b (amb) has a wide spectrum of activity that encompasses the majority of yeast isolates, there have been recent reports suggesting that some yeast isolates exhibit decreased susceptibility to amb. however, in vitro amb susceptibility of yeast species isolates from blood cultures in korea has not been fully surveyed.	2008	18971615
role of sho1p adaptor in the pseudohyphal development, drugs sensitivity, osmotolerance and oxidant stress adaptation in the opportunistic yeast candida lusitaniae.	in yeast, external signals such as high osmolarity or oxidant conditions activate the high osmolarity glycerol (hog) mitogen-activated protein kinase (mapk) cascade pathway, which consists of two upstream branches, i.e. sho1p and sln1p and common downstream elements, including the pbs2p mapk kinase and the hog1p mapk. we recently showed that the candida lusitaniae sln1 gene, potentially encoding a histidine kinase receptor, is crucial for oxidative stress adaptation when the fungus grows as budd ...	2008	19061190
combination of different molecular mechanisms leading to fluconazole resistance in a candida lusitaniae clinical isolate.	we report on the underlying molecular mechanisms likely responsible for the high-level fluconazole resistance in a candida lusitaniae clinical isolate. fluconazole resistance correlated with overexpression of erg11 and of several efflux pump genes, in particular, the orthologs of the candida albicans mdr1, pdr16, cdr1, cdr2, and yor1.	2009	19070454
epidemiology and antifungal susceptibility of bloodstream candida isolates in quebec: report on 453 cases between 2003 and 2005.	between may 2003 and april 2005, a population-based surveillance of candida bloodstream infections was conducted in quebec. a total of 453 episodes of candidemia (464 yeast isolates) from 54 participating hospitals were studied.	2008	19145263
candida lusitaniae fungemia in cancer patients: risk factors for amphotericin b failure and outcome.	candida lusitaniae, a candida species frequently resistant to amphotericin b (amb), is a rare cause of candidemia. the clinical significance of this in vitro resistant phenotype, the risk factors for, and the clinical presentation of c. lusitaniae fungemia in comparison with those of candida albicans have not been completely characterized. we reviewed 13 consecutive cases of c. lusitaniae fungemia in cancer patients and compared them with 41 consecutive cases of c. albicans fungemia (1990-2004). ...	2008	19180749
nonsense and missense mutations in fcy2 and fcy1 genes are responsible for flucytosine resistance and flucytosine-fluconazole cross-resistance in clinical isolates of candida lusitaniae.	the aim of this work was to elucidate the molecular mechanisms of flucytosine (5fc) resistance and 5fc/fluconazole (flc) cross-resistance in 11 genetically and epidemiologically unrelated clinical isolates of candida lusitaniae. we first showed that the levels of transcription of the fcy2 gene encoding purine-cytosine permease (pcp) in the isolates were similar to that in the wild-type strain, 6936. nucleotide sequencing of the fcy2 alleles revealed that 5fc and 5fc/flc resistance could be corre ...	2009	19414575
phylogeny and evolution of the aspartyl protease family from clinically relevant candida species.	aspartyl proteases are a class of enzymes that include the yeast aspartyl proteases and secreted aspartyl protease (sap) superfamilies. several sap superfamily members have been demonstrated or suggested as virulence factors in opportunistic pathogens of the genus candida. candida albicans, candida tropicalis, candida dubliniensis and candida parapsilosis harbour 10, four, eight and three sap genes, respectively. in this work, genome mining and phylogenetic analyses revealed the presence of new ...	2009	19547880
candidaemia with uncommon candida species: predisposing factors, outcome, antifungal susceptibility, and implications for management.	the risk factors for and clinical features of bloodstream infection with uncommon candida spp. (species other than c. albicans, c. glabrata, c. parapsilosis, c. tropicals and c. krusei) are incompletely defined. to identify clinical variables associated with these species that might guide management, 57 cases of candidaemia resulting from uncommon candida spp. were analysed in comparison with 517 episodes of candida albicans candidaemia (2001-2004). infection with uncommon candida spp. (5.3% of ...	2009	19614718
candida albicans hyphal formation and virulence assessed using a caenorhabditis elegans infection model.	candida albicans colonizes the human gastrointestinal tract and can cause life-threatening systemic infection in susceptible hosts. we study here c. albicans virulence determinants using the nematode caenorhabditis elegans in a pathogenesis system that models candidiasis. the yeast form of c. albicans is ingested into the c. elegans digestive tract. in liquid media, the yeast cells then undergo morphological change to form hyphae, which results in aggressive tissue destruction and death of the n ...	2009	19666778
prosthetic valve endocarditis caused by candida lusitaniae, an uncommon pathogen: a case report.	candida lusitaniae was originally described as a human pathogen in 1979 and typically affects immunocompromised patients.	2009	19830215
determination of echinocandin mics for candida species in less than 8 hours: comparison of the rapid susceptibility assay with the clinical and laboratory standards institute's broth microdilution assay.	the echinocandins prevent fungal cell wall synthesis by inhibiting beta-1,3-glucan synthesis, a significant glucose-consuming process. previous studies suggested that echinocandin inhibitory activity is evident within 1 h of exposure. we hypothesized that a susceptibility assay based on glucose consumption may provide clinically useful mics rapidly. the rapid susceptibility assay (rsa), which provides mics in less than 8 h, was compared with the standard broth microdilution susceptibility assay ...	2009	19846655
molecular identification of yeasts associated with traditional egyptian dairy products.	this study aimed to examine the diversity and ecology of yeasts associated with traditional egyptian dairy products employing molecular techniques in yeast identification. a total of 120 samples of fresh and stored domiati cheese, kariesh cheese, and "matared" cream were collected from local markets and examined. forty yeast isolates were cultured from these samples and identified using the restriction-fragment length polymorphism (rflps) of 5.8s-its rdna region and sequencing of the domains d1 ...	2009	19895478
clavispora lusitaniae and chaetomium atrobrunneum as rare agents of cutaneous infection.	we describe the first case of cutaneous infection caused by chaetomium atrobrunneum and clavispora lusitaniae in a one-and-a-half-year-old boy with acute and severe inflammation around his left eyelid. he presented to our outpatient center with a 6-day history and previously ineffective antibacterial therapy. scanning electron microscopy (sem) revealed hyphae and spores were on the surface of the crusty exudates and also penetrated into it, and the microbiology study further showed their charact ...	2010	20020214
development and evaluation of a real-time quantitative pcr assay for detection and enumeration of yeasts of public health interest in dairy products.	yeast contamination is a problem in the food industry as a cause of spoilage. moreover, various species of yeasts are known to be capable of causing opportunistic infections in humans. we have developed a real-time quantitative pcr (qpcr) assay to directly detect and quantify nine emerging opportunistic yeast species (candida albicans, candida glabrata, candida parapsilosis, candida tropicalis, clavispora lusitaniae, filobasidiella neoformans, issatchenkia orientalis, trichosporon asahii, and tr ...	2010	20223545
in vitro susceptibility to seven antifungal agents of candida lusitaniae isolates from an italian university hospital.		2010	20227998
in vitro activity of amphotericin b against candida lusitaniae clinical isolates.		2010	20227999
multidrug resistant yeasts in synanthropic wild birds.	the aim of this study was to investigate the presence of multidrug resistant yeasts in the faeces of synanthropic wild birds from the bangsar suburb of kuala lumpur.	2010	20307325
[susceptibility to fluconazole and voriconazole of candida species isolated from intensive care units patients in medellin, colombia (2001-2007)].	disseminated candidiasis is caused by different candida species and mainly affects immunocompromised patients and those hospitalized in intensive care units (icu).	2010	20450982
analysis of gene evolution and metabolic pathways using the candida gene order browser.	candida species are the most common cause of opportunistic fungal infection worldwide. recent sequencing efforts have provided a wealth of candida genomic data. we have developed the candida gene order browser (cgob), an online tool that aids comparative syntenic analyses of candida species. cgob incorporates all available candida clade genome sequences including two candida albicans isolates (sc5314 and wo-1) and 8 closely related species (candida dubliniensis, candida tropicalis, candida parap ...	2010	20459735
application of an oligonucleotide microarray-based nano-amplification technique for the detection of fungal pathogens.	the traditional techniques for diagnosis of invasive fungal infections in the clinical microbiology laboratory need improvement. these techniques are prone to delay results due to their time-consuming process, or result in misidentification of the fungus due to low sensitivity or low specificity. the aim of this study was to develop a method for the rapid detection and identification of fungal pathogens.	2010	20618094
chromosomal g + c content evolution in yeasts: systematic interspecies differences, and gc-poor troughs at centromeres.	the g + c content at synonymous codon positions (gc3s) in genes varies along chromosomes in most eukaryotes. in saccharomyces cerevisiae, regions of high gc3s are correlated with recombination hot spots, probably due to biased gene conversion. here we examined how gc3s differs among groups of related yeast species in the saccharomyces and candida clades. the chromosomal locations of gc3s peaks and troughs are conserved among four saccharomyces species, but we find that there have been highly con ...	2010	20693156
study of microbial contamination and dosing accuracy of oral dispensers.	the optimal administration of liquid medications requires accurate dose delivery. this is particularly important in the treatment of infants and children, as well as elderly people, who are more sensitive to dosage errors. previous studies revealed significant dosage inaccuracies with measuring spoons. oral syringes are therefore generally recommended instead. there is no data on the efficacy of standard cleaning techniques, and consequently on the degree of microbial contamination associated wi ...	2010	20831530
[mycological analysis of clinical materials of patients receiving total parenteral nutrition].	the most frequent etiological factors of fungal infections in patients receiving total parenteral nutrition (tpn) belong to candida genus of the yeastlike fungi. in the tpn patients the several infectious complications can develop: venous catheter infection, catheter candidemia (fungemia), fungal endocarditis or fungal ophtalmitis. the capability of hydrolytic (proteolytic, lipolytic) enzymes secretion as well as biofilm formation on artificial surfaces are the most important factors of fungal s ...	2010	20873489
a two-step cloning-free pcr-based method for the deletion of genes in the opportunistic pathogenic yeast candida lusitaniae.	we describe a new cloning-free strategy to delete genes in the opportunistic pathogenic yeast candida lusitaniae. we first constructed two ura3δ strains in c. lusitaniae for their use in transformation experiments. one was deleted for the entire ura3 coding sequence; the other possessed a partial deletion within the coding region, which was used to determine the minimum amount of homology required for efficient homologous recombination by double crossing-over of a linear dna fragment restoring u ...	2010	21204058
candida non-albicans versus candida albicans fungemia in the non-neonatal pediatric population.	non-albicans candida (nac) species have been implicated as major pathogens in patients with hospital-acquired candidemia. few studies have investigated the impact of nac fungemia among pediatric patients outside of the neonatal age group.	2011	21372750
[scientific evidence supporting the use of micafungin in the treatment of invasive candidiasis].	micafungin is a semisynthetic lipopeptide developed from coleophoma empetri, which blocks the synthesis of ß-1,3-d-glucan, an essential component of the fungal wall, though non-competitive inhibition of ß-1,3-d-glucan synthetase. micafungin is a dose-dependent candidacidal agent with excellent in vitro efficacy against most candida spp. including species resistant to amphotericin b, such as candida lusitaniae, several azoles, such as c. glabrata or c. krusei, and isolates not susceptible to othe ...	2011	21420572
a two-step cloning-free pcr-based method for the deletion of genes in the opportunistic pathogenic yeast candida lusitaniae.	we describe a new cloning-free strategy to delete genes in the opportunistic pathogenic yeast candida lusitaniae. we first constructed two ura3 ? strains in c. lusitaniae for their use in transformation experiments. one was deleted for the entire ura3 coding sequence; the other possessed a partial deletion within the coding region, which was used to determine the minimum amount of homology required for efficient homologous recombination by double crossing-over of a linear dna fragment restoring ...	2011	21456057
in vitro antifungal susceptibilities of candida isolates from patients with invasive candidiasis in kuala lumpur hospital, malaysia.	the in vitro antifungal susceptibilities of 159 clinical isolates of candida species from patients with invasive candidiasis in kuala lumpur hospital, malaysia, were determined against amphotericin b, fluconazole, voriconazole, itraconazole and caspofungin. the most common species were candida albicans (71 isolates), candida parapsilosis (42 isolates), candida tropicalis (27 isolates) and candida glabrata (12 isolates). the susceptibility tests were carried out using an e-test. the mic breakpoin ...	2011	21459913
development of echinocandin resistance in clavispora lusitaniae during caspofungin treatment.	clavispora lusitaniae is an opportunistic human pathogen responsible for 0.6 to 2% of candidemia. this species is intrinsically susceptible to echinocandins. nevertheless, in this study, development of echinocandin resistance in c. lusitaniae isolates was observed during caspofungin treatment. this resistance resulted from missense mutation in the echinocandin target fks1 gene.	2011	21490186
extensive dna-binding specificity divergence of a conserved transcription regulator.	the dna sequence recognized by a transcription regulator can be conserved across large evolutionary distances. for example, it is known that many homologous regulators in yeasts and mammals can recognize the same (or closely related) dna sequences. in contrast to this paradigm, we describe a case in which the dna-binding specificity of a transcription regulator has changed so extensively (and over a much smaller evolutionary distance) that its cis-regulatory sequence appears unrelated in differe ...	2011	21498688
posaconazole in human serum: a greater pharmacodynamic effect than predicted by the non-protein-bound serum concentration.	it is generally accepted that only the unbound fraction of a drug is pharmacologically active. posaconazole is an antifungal agent with a protein binding of 98-99%. taking into account the degree of protein binding, plasma levels in patients and mic-levels of susceptible strains; it can be assumed that the free concentration of posaconazole sometimes will be too low to exert the expected antifungal effect. the aim was therefore to test the activity of posaconazole in serum in comparison with tha ...	2011	21502622
enzymatic activity of yeasts isolated from the inflamed mammary secretion in dairy cows.	the aim of the study was to evaluate enzymatic activities of yeasts isolated from inflammatory mammary secretion. the yeasts isolated from cows with clinical and sub-clinical mastitis (134 strains) included: candida krusei (62 strains), candida kefyr (48 strains), candida lusitaniae (17 strains) and candida famata (7 strains). the api zym system was used containing substrates to assess 19 hydrolytic enzymes. substantial differences in the number and activity of hydrolyses were demonstrated in in ...	2011	21528713
epidemiology and microbiology of nosocomial pediatric candidemia at a northern indian tertiary care hospital.	the availability and aggressive use of chemotherapeutic and immunosuppressive agents as well as broad-spectrum antibacterial agents have created a large population of patients who are at increased risk of acquiring infections with fungal organisms, especially candida species. present work was undertaken to study the epidemiology and microbiology of candidemia and candida colonization in hospitalized children. a total of 323 suspected cases of septicemia were enrolled, of which blood culture from ...	2011	21533904
in vitro antifungal activity of phenothiazines and their combination with amphotericin b against different candida species.	candidiosis is a mycosis that is currently increasingly affecting the population in consequence of its frequency and the severity of its complications, especially among immunocompromised hosts. in this work, the in vitro anticandidal activities of two phenothiazines (ptzs), chlorpromazine (cpz) and trifluoperazine (tfp), and their combinations with amphotericin b (amb) were tested against 12 different candida strains representing 12 species (candida albicans, candida glabrata, candida guillermon ...	2011	21605196
yeast communities associated with artisanal mezcal fermentations from agave salmiana.	the aims of this work were to characterize the fermentation process of mezcal from san luis potosi, m+®xico and identify the yeasts present in the fermentation using molecular culture-dependent methods (rflp of the 5.8s-its and sequencing of the d1/d2 domain) and also by using a culture-independent method (dgge). the alcoholic fermentations of two separate musts obtained from agave salmiana were analyzed. sugar, ethanol and major volatile compounds concentrations were higher in the first ferment ...	2011	21681584
microbial inactivation in cloudy apple juice by multi-frequency dynashock power ultrasound.	the study determined the efficacy of dynashock wave power ultrasound as an alternative processing technique for apple juice against a number of pathogenic and spoilage microorganisms. the effects of several implicit, intrinsic and extrinsic properties on the dynashock wave inactivation of the microorganisms were also investigated. results showed that acid adaptation increased the resistance of escherichia coli o157:h7 and salmonella spp. but decreased that of listeria monocytogenes. spoilage yea ...	2011	21802974
diversity of bacteria and yeast in the naturally fermented cotton seed and rice beverage produced by brazilian amerindians.	microorganisms associated with the fermentation of cotton seed and rice were studied using a combination of culture-dependent and -independent methods. samples of the cotton seed and rice beverage were collected every 8-áh during the fermentation process for analysis of the microbiota present over 48-áh. the lactic acid bacteria (lab) population reached values of approximately 8.0-álog-ácfu/ml. a total of 162 bacteria and 81 yeast isolates were identified using polyphasic methods. lab (lactobaci ...	2011	21839389
placental and fetal findings in intrauterine candida lusitaniae infection following in vitro fertilization and embryo transfer.	abstract intrauterine infection with non-albicans candida species is rare but can be catastrophic to the fetus. a subset of intrauterine infections with non-albicans candida species have occurred in women who have undergone in vitro fertilization and embryo transfer (ivf-et). we report a case of a 33 year-old healthy woman, pregnant with triplets by in-vitro fertilization, who experienced preterm premature rupture of membranes of fetus a at 16 weeks gestation and subsequently developed oligohy ...	2011	21864122
evaluation of luminex xtag fungal analyte-specific reagents for rapid identification of clinically relevant fungi.	invasive fungal infections (ifi) remain a serious threat to immunocompromised hosts. current diagnostic methods, including fungal culture and antigen detection, are slow and often lack specificity. rapid diagnostic tools with increased sensitivity and specificity could improve the care of patients with ifi. recently, luminex molecular diagnostics (toronto, canada) developed 23 analyte-specific reagents (asrs) for the detection of the most common clinically relevant fungi. this study's objective ...	2011	21880976
usefulness of mcrapd for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by candida lusitaniae.	a case report of ventriculoperitoneal shunt infection caused by candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. seven isolates recovered subsequently from the cerebrospinal fluid were studied retrospectively. to confirm identity, isolates were typed using pulsed-field gel electrophoresis and melting curve of random amplified polymorphic dna (mcrapd). further, the ability t ...	2011	21894550
defining pheromone-receptor signaling in candida albicans and related asexual candida species.	candida albicans is an important human fungal pathogen in which sexual reproduction is under the control of the novel white-opaque switch. opaque cells are the mating-competent form, whereas white cells do not mate but can still respond to pheromones, resulting in biofilm formation. in this study, we first define the domains of the α-pheromone receptor ste2 that are necessary for signaling in both white and opaque forms. both cell states require the ic loop 3 (ic3) and the c-terminal tail of ste ...	2011	21998194
candida lusitaniae discitis after discogram in an immunocompetent patient.	discitis or epidural abscess after discogram is a rare but known complication. it is more commonly bacterial; however, fungal discitis has been previously reported in immunocompromised patients. the management of fungal discitis in immunocompetent patients is rarely presented or addressed in the literature.	2011	22005083
proanthocyanidin-rich extracts from cranberry fruit ( vaccinium macrocarpon ait.) selectively inhibit the growth of human pathogenic fungi candida spp. and cryptococcus neoformans.	cranberry ( vaccinium macrocarpon ) has been shown in clinical studies to reduce infections caused by escherichia coli and other bacteria, and proanthocyanidins are believed to play a role. the ability of cranberry to inhibit the growth of opportunistic human fungal pathogens that cause oral, skin, respiratory, and systemic infections has not been well-studied. fractions from whole cranberry fruit were screened for inhibition of five candida species and cryptococcus neoformans , a causative ...	2011	22066866
identification and characterization of a novel legume-like lectin cdna sequence from the red marine algae gracilaria fisheri.	a legume-type lectin (l-lectin) gene of the red algae gracilaria fisheri (gfl) was cloned by rapid amplification of cdna ends (race). the full-length cdna of gfl was 1714 bp and contained a 1542 bp open reading frame encoding 513 amino acids with a predicted molecular mass of 56.5 kda. analysis of the putative amino acid sequence with ncbi-blast revealed a high homology (30-68%) with legume-type lectins (l-lectin) from griffithsia japonica, clavispora lusitaniae, acyrthosiphon pisum, tetraodon n ...	2011	22116281
ocular involvement in hospitalized patients with candidemia: analysis at a boston tertiary care center.	to study the prevalence, microbial profile, and risk factors for ocular involvement in patients with candidemia.	2012	22409562
determination of the effect of nd:yag laser irradiation through dentinal tubules on several oral pathogens.	the effects of nd:yag laser irradiations at different power settings on several oral pathogens were evaluated. a total of 252 dentin samples were divided into seven groups consisting of 36 dentin specimens each. in each group, 9 of the 36 specimens were used as controls, thereby including a control in every group. the remaining 27 specimens were divided into three subgroups consisting of nine specimens according to different nd:yag laser settings (1.5, 1.8, and 2 w). each group was inoculated on ...	2013	22767323
yeasts from colombian kumis as source of peptides with angiotensin i converting enzyme (ace) inhibitory activity in milk.	this study investigated the possibility of using yeast strains in fermented milks to obtain products with high angiotensin i-converting enzyme (ace) inhibitory activity and low bitter taste. ninety-three yeast strains isolated from colombian kumis in different geographic regions were molecularly identified, and their milk fermentation performances were determined. molecular identification evidenced that galactomyces geotrichum, pichia kudriavzevii, clavispora lusitaniae and candida tropicalis, w ...	2012	22938834
calcineurin is required for pseudohyphal growth, virulence, and drug resistance in candida lusitaniae.	candida lusitaniae is an emerging fungal pathogen that infects immunocompromised patients including hiv/aids, cancer, and neonatal pediatric patients. though less prevalent than other candida species, c. lusitaniae is unique in its ability to develop resistance to amphotericin b. we investigated the role of the calcium-activated protein phosphatase calcineurin in several virulence attributes of c. lusitaniae including pseudohyphal growth, serum survival, and growth at 37°c. we found that calcine ...	2012	22952924
the dominant microbial community associated with fermentation of obushera (sorghum and millet beverages) determined by culture-dependent and culture-independent methods.	obushera includes four fermented cereal beverages from uganda namely: obutoko, enturire, ekitiribita and obuteire, whose microbial diversity has not hitherto been fully investigated. knowledge of the microbial diversity and dynamics in these products is crucial for understanding their safety and development of appropriate starter cultures for controlled industrial processing. culture-dependent and culture-independent techniques including denaturating gradient gel electrophoresis (dgge) and mixed ...	2012	23141639
rapid and accurate identification of isolates of candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca).	rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. we evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca) as an identification tool for distinguishing between 16 candida spp., i.e. candida albicans, candida bracarensis, candida dubliniensis, candida famata, candida glabrata, candida guilliermondii, candida inconspicua, candida k ...	2013	23142490
[yeast colonization of urinary catheters and the significance of biofilm formation].	urinary catheters are colonized by a wide range of microorganisms, including numerous yeasts. the catheters are usually colonized by more microbial species forming a community - multispecies biofilm. catheter colonization usually does not affect the patient's clinical status in any significant way. on the other hand, the biofilm can become a source of endogenous infection and its presence can affect functionality of the catheter and formation of urinary stones. material a	2012	23172023
examination of the in vitro fungicidal activity of echinocandins against candida lusitaniae by time-killing methods.	candida lusitaniae fungaemia, although infrequent (1%), is more common in immunocompromised patients than candida albicans. although infections produced by candida spp. are therapeutic targets for treatment with echinocandins, little information is available regarding their killing kinetics against c. lusitaniae. the objectives of this study were to determine the killing kinetics of anidulafungin, micafungin and caspofungin against four blood isolates of c. lusitaniae by time-kill methodology.	2013	23228935
oral candida species in head and neck cancer patients treated by radiotherapy.	this paper aimed to identify and quantify candida on head and neck irradiated patients with two comparative elderly populations.	2013	23260345
the diversity and antifungal susceptibility of the yeasts isolated from coconut water and reconstituted fruit juices in brazil.	the aims of this study were to characterise the yeasts present in the reconstituted fruit juices and coconut water extracted with "coconut machines", both collected from commercial outlets in a brazilian city, and to investigate the antifungal resistance of isolates from these beverages that were able to grow at 37°c. the yeast population counts in the coconut water samples ranged from 1.7 to >6.5logcfu/ml, and in the reconstituted fruit juices, the counts ranged from 1.5 to >5.5logcfu/ml. aureo ...	2012	23290225
[identification of three candida africana strains in senegal].	the frequency of candidiasis has increased dramatically in recent years. candida albicans is the most common species. however, other species which are pathogenic and resistant to usual antifungal agents beginning to emerge. these include candida dubliniensis and candida africana, which share morphological similarities with candida albicans. thus, it is of interest to correctly identify the fungal isolates.	2012	23518168
species-specific and drug-specific differences in susceptibility of candida biofilms to echinocandins: characterization of less common bloodstream isolates.	candida species other than candida albicans are increasingly recognized as causes of biofilm-associated infections. this is a comprehensive study that compared the in vitro activities of all three echinocandins against biofilms formed by different common and infrequently identified candida isolates. we determined the activities of anidulafungin (anid), caspofungin (cas), and micafungin (mfg) against planktonic cells and biofilms of bloodstream isolates of c. albicans (15 strains), candida paraps ...	2013	23529739
yeasts from autochthonal cheese starters: technological and functional properties.	the aim of this work was to identify 20 yeasts isolated from autochthonal cheese starters and evaluate their technological and functional properties.	2013	23600736
determination of yeast diversity in ogi, mawè, gowé and tchoukoutou by using culture-dependent and -independent methods.	the maize based ogi and mawè and the sorghum based gowé and tchoukoutou are traditional, spontaneously fermented products widely consumed by the population of benin (west africa). yeast occurrence in the products, as sold on local markets at different locations, was studied using a combination of culture-dependent and independent methods. number of yeasts is varied from 3.75 log10 colony forming units (cfu)/g for ogi to 5.60 log10 cfu/g for tchoukoutou. isolated yeasts (236) were identified base ...	2013	23727651
activity of four antimicrobial cocktails for tissue allograft decontamination against bacteria and candida spp. of known susceptibility at different temperatures.	several antimicrobial cocktail solutions of differing composition and concentrations are widely used to decontaminate viable banked tissue allografts at different temperatures and times of exposure. we compared the efficiency of four cocktails comprising nine antimicrobials to kill suspensions of a panel of 27 strains of 13 bacterial species, and 3 candida spp. at 4, 22 and 37 °c for 24 h. all but one bacterial strains were susceptible to one or more of the agents tested individually at concentr ...	2014	23765096
evaluation of the microbial community, acidity and proximate composition of akamu, a fermented maize food.	akamu is a lactic acid-fermented cereal-based food that constitutes a major infant complementary food in most west african countries. the identities of lab populations from dgge analysis and conventionally isolated lab and yeasts from traditionally fermented akamu were confirmed by pcr sequencing analysis. the relationships between ph, acidity and lactic acid levels and proximate composition of the akamu samples were investigated.	2014	23765582
survey of mycotic mastitis in dairy cows from heilongjiang province, china.	a survey of the prevalence rate, pathogenic subspecies, and risk factors of mycotic mastitis in dairy cows from heilongjiang province, china, was conducted. milk samples from 412 cows with chronic mastitis were collected and cultured on 8 % sheep blood agar, macconkey agar, and sabouraud agar with chloramphenicol. counting of the morphologically distinct colonies was performed, as well as the isolation and identification of organisms through phenotypical and physiological criteria. four hundred ...	2013	23813119
comparison of the vitek 2 yeast susceptibility system with clsi microdilution for antifungal susceptibility testing of fluconazole and voriconazole against candida spp., using new clinical breakpoints and epidemiological cutoff values.	a commercially available, fully automated yeast susceptibility test system (vitek 2; biomérieux, marcy d'etoile, france) was compared in 3 different laboratories with the clinical and laboratory standards institute (clsi) reference microdilution (bmd) method by testing 2 quality control strains, 10 reproducibility strains, and 425 isolates of candida spp. against fluconazole and voriconazole. reference clsi bmd mic endpoints and vitek 2 mic endpoints were read after 24 hours and 9.1-27.1 hours i ...	2013	23870164
an in vitro study on the anti-adherence effect of brucea javanica and piper betle extracts towards oral candida.	the adherence of candida to mucosal surfaces is the initial step for successful invasive process of the oral cavity. the study aimed to investigate the effect of two plant extracts on the non-specific and specific bindings of oral candida.	2013	23915676
host-pathogen interaction and signaling molecule secretion are modified in the dpp3 knockout mutant of candida lusitaniae.	candida lusitaniae is an emerging opportunistic yeast and an attractive model to discover new virulence factors in candida species by reverse genetics. our goal was to create a dpp3δ knockout mutant and to characterize the effects of this gene inactivation on yeast in vitro and in vivo interaction with the host. the secretion of two signaling molecules in candida species, phenethyl alcohol (pea) and tyrosol, but not of farnesol was surprisingly altered in the dpp3δ knockout mutant. no and reacti ...	2014	24191303
the deacetylase sir2 from the yeast clavispora lusitaniae lacks the evolutionarily conserved capacity to generate subtelomeric heterochromatin.	deacetylases of the sir2 or sirtuin family are thought to regulate life cycle progression and life span in response to nutrient availability. this family has undergone successive rounds of duplication and diversification, enabling the enzymes to perform a wide variety of biological functions. two evolutionarily conserved functions of yeast sir2 proteins are the generation of repressive chromatin in subtelomeric domains and the suppression of unbalanced recombination within the tandem rdna array. ...	2013	24204326
antimicrobial activity of nisin, thymol, carvacrol and cymene against growth of candida lusitaniae.	yeasts are tolerant to acid ph values, are able to grow in anaerobic media and have minimum nutrition requirements. these capabilities enable them to survive and even grow in foods prepared from acid fruits or vegetables. among yeasts, candida is one of the genus most frequently isolated from fruit juices. bacteriocins and essential oils from spices and aromatic herbs are an alternative to preservatives and other technological treatments and have the advantage that their natural origins do not l ...	2015	24293188
antifungal susceptibility and growth inhibitory response of oral candida species to brucea javanica linn. extract.	candida species have been associated with the emergence of resistant strains towards selected antifungal agents. plant products have been used traditionally as alternative medicine to ease candidal infections. the present study was undertaken to investigate the antifungal susceptibility patterns and growth inhibiting effect of brucea javanica seeds extract against candida species.	2013	24305010
in vitro pharmacodynamics and in vivo efficacy of fluconazole, amphotericin b and caspofungin in a murine infection by candida lusitaniae.	the in vitro activities of fluconazole (flc), amphotericin b (amb) and caspofungin (csp) were evaluated against three isolates of candida lusitaniae using time-kill curves. amb showed in vitro fungicidal activity, whilst flc and csp exerted mainly strain-dependent fungistatic activity. the in vivo efficacies of the three drugs were evaluated in a murine model of disseminated infection. the doses administered were flc 50 mg/kg/day, amb 0.8 mg/kg/day and csp 5 mg/kg/day. all three drugs were able ...	2014	24360922
molecular identification and antifungal susceptibility of yeast isolates causing fungemia collected in a population-based study in spain in 2010 and 2011.	we report the molecular identifications and antifungal susceptibilities of the isolates causing fungemia collected in the candipop population-based study conducted in 29 spanish hospitals. a total of 781 isolates (from 767 patients, 14 of them having mixed fungemia) were collected. the species found most frequently were candida albicans (44.6%), candida parapsilosis (24.5%), candida glabrata (13.2%), candida tropicalis (7.6%), candida krusei (1.9%), candida guilliermondii (1.7%), and candida lus ...	2014	24366741
convergent evolution of a fused sexual cycle promotes the haploid lifestyle.	sexual reproduction is restricted to eukaryotic species and involves the fusion of haploid gametes to form a diploid cell that subsequently undergoes meiosis to generate recombinant haploid forms. this process has been extensively studied in the unicellular yeast saccharomyces cerevisiae, which exhibits separate regulatory control over mating and meiosis. here we address the mechanism of sexual reproduction in the related hemiascomycete species candida lusitaniae. we demonstrate that, in contras ...	2014	24390351
yeasts and hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil.	the aim of this work was to study the yeast populations and the main hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. forty-two water buffalo mozzarella samples were purchased from retail outlets in belo horizonte. in addition, five samples of consecutive starter cultures, curd before acidification, acidified curd and mozzarella were collected at an industry in the city of oliveira. only three of the five water samples analyz ...	2013	24516436
an ene reductase from clavispora lusitaniae for asymmetric reduction of activated alkenes.	a putative ene reductase gene from clavispora lusitaniae was heterologously overexpressed in escherichia coli, and the encoded protein (cler) was purified and characterized for its biocatalytic properties. this nadph-dependent flavoprotein was identified with reduction activities toward a diverse range of activated alkenes including conjugated enones, enals, maleimide derivative and α,β-unsaturated carboxylic esters. the purified cler exhibited a relatively high activity of 7.3 u mg(prot)⁻¹ for ...	2014	24564901
the development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis.	accurate, rapid and economical fungal species identification has been a major aim in mycology. in this study, our goal was to examine the feasibility of a high-resolution melting curve analysis (hrma) of internal transcribed regions its1 and its2 in ribosomal dna (rdna) for a rapid, simple and inexpensive differentiation of eight clinically relevant candida species (candida albicans, candida glabrata, candida parapsilosis, candida krusei, candida tropicalis, candida guilliermondii, candida dubli ...	2014	24628973
acute limb ischemia due to candida lusitaniae aortic valve endocarditis.	we present a case of a 35-year-old male with history of intravenous heroin use, who presented with acute limb ischemia and was found to have candida lusitaniae endocarditis. after an urgent intervention to salvage the right lower extremity, successful outcome of his infection was achieved by a combined surgical and antifungal treatment.	2014	24751340
long-term follow up of complete bladder exstrophy repair. a case report.	bladder exstrophy is a congenital anomaly which is not always successfully managed by surgery. major goals of surgical intervention in such cases are preservation of normal renal function, development of adequate bladder function and urinary continence and avoidance of future urinary tract infections. we present 5-year data on a patient who underwent complete repair of the bladder exstrophy.	2014	24812925
deletion of the uracil permease gene confers cross-resistance to 5-fluorouracil and azoles in candida lusitaniae and highlights antagonistic interaction between fluorinated nucleotides and fluconazole.	we characterized two additional membrane transporters (fur4p and dal4p) of the nucleobase cation symporter 1 (ncs1) family involved in the uptake transport of pyrimidines and related molecules in the opportunistic pathogenic yeast candida lusitaniae. simple and multiple null mutants were constructed by gene deletion and genetic crosses. the function of each transporter was characterized by supplementation experiments, and the kinetic parameters of the uptake transport of uracil were measured usi ...	2014	24867971
caspofungin at catheter lock concentrations eradicates mature biofilms of candida lusitaniae and candida guilliermondii.	the antibiofilm activities of caspofungin, anidulafungin, micafungin, and liposomal amphotericin b were studied against candida lusitaniae, candida guilliermondii, and a candida albicans control strain. while anidulafungin and micafungin (0.007 to 2,048 mg/liter) showed reduced activity against biofilms of both test species, caspofungin displayed concentration-dependent antibiofilm activity, reaching complete and persistent eradication at concentrations achievable during lock therapy (512 to 2,0 ...	2014	24890585
uncommon opportunistic yeast bloodstream infections from qatar.	eleven uncommon yeast species that are associated with high mortality rates irrespective of antifungal therapy were isolated from 17/187 (201 episodes) pediatric and elderly patients with fungemia from qatar. the samples were taken over a 6-year period (january 2004-december 2010). isolated species included kluyveromyces marxianus, lodderomyces elongisporus, lindnera fabianii, candida dubliniensis, meyerozyma guilliermondii, candida intermedia, pichia kudriavzevii, yarrowia lipolytica, clavispor ...	2014	24934803
isavuconazole and nine comparator antifungal susceptibility profiles for common and uncommon candida species collected in 2012: application of new clsi clinical breakpoints and epidemiological cutoff values.	the in vitro activity of isavuconazole and nine antifungal comparator agents was assessed using reference broth microdilution methods against 1,421 common and uncommon species of candida from a 2012 global survey. isolates were identified using chromagar, biochemical methods and sequencing of its and/or 28s regions. candida spp. were classified as either susceptible or resistant and as wild type (wt) or non-wt using clsi clinical breakpoints or epidemiological cutoff values, respectively, for th ...	2014	24952015
digestive fungal flora in asymptomatic subjects in bobo-dioulasso, burkina faso.	to identify candida species in asymptomatic subjects in bobo-dioulasso (burkina faso) by the matrix-assisted laser desorption ionization-time of flight mass spectrometry.	2014	25183337
eukaryote culturomics of the gut reveals new species.	the repertoire of microeukaryotes in the human gut has been poorly explored, mainly in individuals living in northern hemisphere countries. we further explored this repertoire using pcr-sequencing and culture in seven individuals living in four tropical countries. a total of 41 microeukaryotes including 38 different fungal species and three protists were detected. four fungal species, davidiella tassiana, davidiella sp., corticiaceae sp., and penicillium sp., were uniquely detected by culture; 2 ...	2014	25210972
candida and other yeasts of clinical importance in aseer region, southern saudi arabia. presentation of isolates from the routine laboratory setting.	to isolate, identify, and determine the prevalence of candida and other yeasts of clinical importance in aseer region, saudi arabia.	2014	25316465
[trend of susceptibility testing of clinical isolates of candida species from aseptic samples in hospitals in saitama prefecture].	we investigated the susceptibility of candida species from clinical aseptic samples, including blood, at some hospitals in saitama prefecture. candida spp. detected from aseptic samples in the 6 institutes in saitama prefecture from november 2007 to july 2011 were studied. the number of isolates was 85, which are 43 (50.6%) of candida albicans, 24 (28.2%) of candida parapsilosis, 5 (5.9%) of candida glabrata, 5 (5.9%) of candida tropicalis, 4 (4.7%) of candida guilliermondii, 2 (2.4%) of candida ...	2014	25420317
phenotypic switching of candida guilliermondii is associated with pseudohyphae formation and antifungal resistance.	switching between two cell types in fungi is called phenotypic switching, and it is commonly observed in pathogenic yeast. candida lusitaniae undergoes antifungal resistance-associated phenotypic switching and results in three colony colors: light brown, brown and dark brown. in this study, we included c. lusitaniae as control. this study had two objectives. first, we wanted to evaluate whether also a prevalent human pathogen c. guilliermondii can undergo phenotypic switching. second, our aim wa ...	2015	25481846
a fox2-dependent fatty acid ß-oxidation pathway coexists both in peroxisomes and mitochondria of the ascomycete yeast candida lusitaniae.	it is generally admitted that the ascomycete yeasts of the subphylum saccharomycotina possess a single fatty acid ß-oxidation pathway located exclusively in peroxisomes, and that they lost mitochondrial ß-oxidation early during evolution. in this work, we showed that mutants of the opportunistic pathogenic yeast candida lusitaniae which lack the multifunctional enzyme fox2p, a key enzyme of the ß-oxidation pathway, were still able to grow on fatty acids as the sole carbon source, suggesting that ...	2014	25486052
[trend of susceptibility testing of clinical isolates of candida species from aseptic samples in hospitals in saitama prefecture].	we investigated the susceptibility of candida species from clinical aseptic samples, including blood, at some hospitals in saitama prefecture. candida spp. detected from aseptic samples in the 6 institutes in saitama prefecture from november 2007 to july 2011 were studied. the number of isolates was 85, which are 43 (50.6%) of candida albicans, 24 (28.2%) of candida parapsilosis, 5 (5.9%) of candida glabrata, 5 (5.9%) of candida tropicalis, 4 (4.7%) of candida guilliermondii, 2 (2.4%) of candida ...	2014	25507301
genetic diversity of clavispora lusitaniae isolated from agave fourcroydes lem, as revealed by dna fingerprinting.	this study characterized clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (agave fourcroydes) spirit produced in yucatan, mexico using a molecular technique. sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. nine different versions of the divergent d1/d2 domain of the large-subunit ribosomal dna sequence were identified among the c. lusitaniae strains. the grea ...	2015	25557477
[molecular identification of candida lusitaniae in lower respiratory tract infection].	candida lusitaniae is a yeast that has emerged as a low frequency nosocomial pathogen in deep infections. although it usually shows in vitro susceptibility to all antifungal agents, in vivo resistance to amphotericin b has been observed in several clinical cases. therefore, its early identification in the course of therapy is important. we report the isolation of c. lusitaniae as an etiologic agent of a lower respiratory tract infection in a male patient. urine and sputum cultures were negative ...	2015	25576413
identification of some yeasts by fatty acid profiles.	178 yeasts isolated from various foods were identified using id32 c strip; saccharomyces cerevisiae (47), rhodotorula mucilaginosa (19), candida kefyr (33), candida krusei (37), candida lusitaniae (28), cndida lipolytica (14). the yeasts were harvested, saponified, derivatized, and extracted, and fatty acid analysis was performed by gas chromatography mass spectrometry. the yeast lipids contained decanoic acid (c10:0), luaric acid (c12:0), myristoleic acid (c14:1), myristic acid (c14:0), pentade ...	2014	25804068
genetic diversity of medically important and emerging candida species causing invasive infection.	genetic variation in the ribosomal dna (rdna) internal transcribed spacer (its) region has been studied among fungi. however, the numbers of its sequence polymorphisms in the various candida species and their associations with sources of invasive fungal infections remain poorly investigated. here, we characterized the intraspecific and interspecific its diversity of candida spp. strains collected from patients with bloodstream or oroesophageal candidiasis.	2015	25887032
antifungal susceptibility profiles of bloodstream yeast isolates by sensititre yeastone over nine years at a large italian teaching hospital.	sensititre yeastone (syo) is an affordable alternative to the clinical and laboratory standards institute (clsi) reference method for antifungal susceptibility testing. in this study, the mics of yeast isolates from 1,214 bloodstream infection episodes, generated by syo during hospital laboratory activity (january 2005 to december 2013), were reanalyzed using current clsi clinical breakpoints/epidemiological cutoff values to assign susceptibility (or the wild-type [wt] phenotype) to systemic ant ...	2015	25896705