Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| failure of atovaquone/proguanil to prevent plasmodium ovale malaria in traveler returning from cameroon. | we report a case of a patient returning from cameroon who developed plasmodium ovale malaria, despite atovaquone/proguanil (ap, malarone) prophylaxis, which is widely used for the prevention of chloroquine-resistant malaria. ap is indeed active only on schizont blood forms of p. ovale but not against liver-stage hypnozoites and does not realize effective prophylaxis against delayed onset of p. ovale malaria. hence, this case illustrates the risk of failure with malarone for the prophylaxis of p. ... | 2008 | 18486067 |
| an 8-year survey on the occurrence of imported malaria in a nonendemic area by microscopy and molecular assays. | our study aimed to describe the occurrence of imported malaria in a nonendemic area (parma, italy) during the period 2000 to 2007, comparing the data obtained by microscopy and molecular assays targeting plasmodial 18s subunit rrna gene. the prevalence of imported malaria in parma was 21.8% by microscopy and 22.7% by polymerase chain reaction (pcr). plasmodium falciparum accounted for 81.1% of the cases, followed by plasmodium ovale (8.8%), plasmodium vivax (3.8%), and plasmodium malariae (1.9%) ... | 2008 | 18501548 |
| malaria chemoprophylaxis: strategies for risk groups. | the risk of malaria for travelers varies from region to region and depends on the intensity of transmission, the duration of the stay in the area of endemicity, the style of travel, and the efficacy of preventive measures. the decision to recommend chemoprophylaxis to travelers to areas with a low risk of malarial infection is especially difficult because the risk of infection must be balanced with the risk of experiencing side effects. if the risk of side effects by far exceeds the risk of infe ... | 2008 | 18625682 |
| identification of differentially expressed genes after acute exposure to bulaquine (cdri 80/53) in mice liver. | therapeutic agents derived from 8-aminoquinoline possess potent activity against hepatic stages of plasmodia. bulaquine (cdri 80/53), an enamine analogue of primaquine and a relatively new derivative of 8-aminioquinoline, synthesized at the central drug research institute, lucknow, india, has shown promising activity against hypnozoites of plasmodium vivax and plasmodium ovale. moreover, it has been found to be three to four times safer than primaquine in pre-clinical studies. in this study, glo ... | 2008 | 18684221 |
| malaria in refugees from tanzania--king county, washington, 2007. | recent immigrants and refugees constitute a substantial proportion of malaria cases in the united states, accounting for nearly one in 10 imported malaria cases involving persons with known resident status in 2006. this report describes three cases of plasmodium falciparum malaria and two cases of plasmodium ovale malaria that occurred during june 27-october 15, 2007 in king county, washington. the infections were diagnosed in burundian refugees who had recently arrived in the united states from ... | 2008 | 18701876 |
| [malaria diagnosis: thickdrop and bloodsmear examination, and rapid test]. | 2008 | 18711803 | |
| failure to detect plasmodium vivax in west and central africa by pcr species typing. | plasmodium vivax is estimated to affect 75 million people annually. it is reportedly absent, however, from west and central africa due to the high prevalence of the duffy negative phenotype in the indigenous populations. despite this, non-african travellers consistently return to their own countries with p. vivax malaria after visiting this region. an attempt was made, therefore, to detect the presence of p. vivax parasites in blood samples collected from the indigenous populations of west and c ... | 2008 | 18783630 |
| acute respiratory distress syndrome in a case of plasmodium ovale malaria. | acute respiratory distress syndrome is a well-known complication in plasmodium falciparum infection. it is less frequently described in plasmodium vivax, and only one case is reported in plasmodium ovale. here we present the second description of this pulmonary complication in a p. ovale acute infection. | 2008 | 18784231 |
| pcr-based detection of plasmodium in anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods. | detection of the four malaria-causing plasmodium species (plasmodium falciparum, plasmodium vivax, plasmodium ovale and plasmodium malariae) within their mosquito hosts is an essential component of vector control programmes. several pcr protocols have been developed for this purpose. many of these methods, while sensitive, require multiple pcr reactions to detect and discriminate all four plasmodium species. in this study a new high-throughput assay was developed and compared with three previous ... | 2008 | 18793416 |
| evaluation and optimization of membrane feeding compared to direct feeding as an assay for infectivity. | malaria parasite infectivity to mosquitoes has been measured in a variety of ways and setting, includind direct feeds of and/or membrane feeding blood collected from randomly selected or gametocytemic volunteers. anopheles gambiae s.l is the main vector responsible of plasmodium falciparum transmission in bancoumana and represents about 90% of the laboratory findings, whereas plasmodium malariae and plasmodium ovale together represent only 10%. | 2008 | 19055715 |
| prevalence of plasmodium spp. in malaria asymptomatic african migrants assessed by nucleic acid sequence based amplification. | malaria is one of the most important infectious diseases in the world. although most cases are found distributed in the tropical regions of africa, asia, central and south americas, there is in europe a significant increase in the number of imported cases in non-endemic countries, in particular due to the higher mobility in today's society. | 2009 | 19138412 |
| high sensitivity detection of plasmodium species reveals positive correlations between infections of different species, shifts in age distribution and reduced local variation in papua new guinea. | when diagnosed by standard light microscopy (lm), malaria prevalence can vary significantly between sites, even at local scale, and mixed species infections are consistently less common than expect in areas co-endemic for plasmodium falciparum, plasmodium vivax and plasmodium malariae. the development of a high-throughput molecular species diagnostic assay now enables routine pcr-based surveillance of malaria infections in large field and intervention studies, and improves resolution of species ... | 2009 | 19284594 |
| [false-negative result with the rapid diagnostic test in plasmodium ovale infection]. | 2009 | 19386395 | |
| towards high-throughput molecular detection of plasmodium: new approaches and molecular markers. | several strategies are currently deployed in many countries in the tropics to strengthen malaria control toward malaria elimination. to measure the impact of any intervention, there is a need to detect malaria properly. mostly, decisions still rely on microscopy diagnosis. but sensitive diagnosis tools enabling to deal with a large number of samples are needed. the molecular detection approach offers a much higher sensitivity, and the flexibility to be automated and upgraded. | 2009 | 19402894 |
| pathological rupture of the spleen in malaria: analysis of 55 cases (1958-2008). | splenic rupture during acute malaria is rare but underreported. because splenic rupture occurs mostly in non-immune adults, ongoing malaria elimination efforts may paradoxically increase the proportion of plasmodium-infected patients suffering from this life-threatening complication. the pathogenesis and optimal patient management are still debated. | 2009 | 19411041 |
| test characteristics of two rapid antigen detection tests (sd fk50 and sd fk60) for the diagnosis of malaria in returned travellers. | two malaria rapid diagnostic tests were evaluated in a travel clinic setting: the sd fk50 malaria ag plasmodium falciparum test (a two-band test) and the sd fk60 malaria ag p. falciparum/pan test (a three-band test). | 2009 | 19416497 |
| preliminary study of malaria incidence in nouakchott, mauritania. | malaria is one of the main motives for outpatient consultation and hospitalization in mauritania. however, its incidence remains unclear because of diagnostic problems and insufficient epidemiological data. | 2009 | 19416545 |
| chimpanzee malaria parasites related to plasmodium ovale in africa. | since the 1970's, the diversity of plasmodium parasites in african great apes has been neglected. surprisingly, p. reichenowi, a chimpanzee parasite, is the only such parasite to have been molecularly characterized. this parasite is closely phylogenetically related to p. falciparum, the principal cause of the greatest malaria burden in humans. studies of malaria parasites from anthropoid primates may provide relevant phylogenetic information, improving our understanding of the origin and evoluti ... | 2009 | 19436742 |
| evaluation of the sd fk70 malaria ag plasmodium vivax rapid diagnostic test in a non-endemic setting. | for clinical and epidemiological reasons, it is interesting to diagnose non-falciparum malaria to the species level. this retrospective study assessed the performance of the sd bioline malaria antigen pv test (fk70), a two-band immunochromatographic test detecting plasmodium vivax-specific lactate dehydrogenase, on samples of international travellers in a non-endemic setting. | 2009 | 19519915 |
| alpha-substituted norstatines as the transition-state mimic in inhibitors of multiple digestive vacuole malaria aspartic proteases. | the impact of moving the p1 side-chain from the beta-position to the alpha-position in norstatine-containing plasmepsin inhibitors was investigated, generating two new classes of tertiary alcohol-comprising alpha-benzylnorstatines and alpha-phenylnorstatines. twelve alpha-substituted norstatines were designed, synthesized and evaluated for their inhibitory potencies against plasmepsin ii and the plasmepsin iv orthologues (pm4) present in the digestive vacuole of all four plasmodium species causi ... | 2009 | 19635672 |
| implementation of a novel pcr based method for detecting malaria parasites from naturally infected mosquitoes in papua new guinea. | detection of plasmodium species in mosquitoes is important for designing vector control studies. however, most of the pcr-based detection methods show some potential limitations. the objective of this study was to introduce an effective pcr-based method for detecting plasmodium vivax and plasmodium falciparum from the field-caught mosquitoes of papua new guinea. | 2009 | 19646275 |
| longitudinal survey of malaria morbidity over 10 years in saharevo (madagascar): further lessons for strengthening malaria control. | madagascar has been known for having bio-geo-ecological diversity which is reflected by a complex malaria epidemiology ranging from hyperendemic to malaria-free areas. malaria-related attacks and infection are frequently recorded both in children and adults living in areas of low malaria transmission. to integrate this variability in the national malaria control policy, extensive epidemiological studies are required to up-date previous records and adjust strategies. | 2009 | 19660116 |
| value of liu's stain in rapid diagnosis of plasmodium ovale infection. | 2009 | 19751654 | |
| [malaria in croatia in the period between 1987 to 2006]. | a retrospective study about the incidence of malaria in croatia in the period from 1987 to 2006 based on the official data of the croatian national institute of public health. in this period there were 201 cases of malaria registered in croatia. the majority (79.6%) were imported from africa, a significantly lower number from asia (17%), and several cases from south america or from unknown locations. one case ended in death. the causative agents determined are plasmodium falciparum (64.7%), plas ... | 2009 | 19769279 |
| [imported malaria in northern central morocco, 1997-2007]. | after the elimination of autochthonous malaria in morocco in 2004, control of imported malaria, based on epidemiological monitoring of the parasite and vector control, has become a priority. this retrospective study concerns imported malaria cases identified by optical microscopy at the regional epidemiological diagnostic laboratory of the regional health department of fes from 1997 through 2007. the results obtained showed that 56 of 68 (82%) samples examined were positive. women accounted for ... | 2009 | 19801352 |
| comparison of three real-time pcr methods with blood smears and rapid diagnostic test in plasmodium sp. infection. | in cases of malaria, rapid and accurate diagnosis of plasmodium sp. is essential. in this study three different quantitative, real-time pcr methods were compared with routine methods used for malaria diagnosis. a comparative study was conducted prospectively in the laboratories of montpellier and nîmes university hospitals. the methods used for routine diagnostic malaria testing consisted of microscopic examination of giemsa-stained blood smears and rapid diagnostic tests. three quantitative rea ... | 2010 | 19840032 |
| evaluation of rapid diagnostic tests for malaria case management in gabon. | a laboratory-confirmed diagnosis is the basis of malaria case management. rapid diagnostic tests (rdts) create new opportunities for improved care in endemic areas. diagnostic performance of optimal-it and acon was assessed in comparison with microscopy at 2 sites in gabon. between february 2008 and january 2009, 2125 febrile children under 11 years old were diagnosed using microscopy and rdts. plasmodial infection was detected more frequently using acon (27%) and optimal-it (27%) compared to mi ... | 2010 | 19846265 |
| kir3dl1/s1 genotypes and kir2ds4 allelic variants in the ab kir genotypes are associated with plasmodium-positive individuals in malaria infection. | the importance of innate immunity in malaria has been suggested for early protection from maturation and multiplication of plasmodium parasites injected via infected mosquitoes. in this study, the killer cell immunoglobulin-like receptor (kir) genes in innate immunity were investigated for an association with malaria in the comparison between plasmodium-positive and plasmodium-negative melanesian individuals in the solomon islands, one of the most hyperendemic malaria regions in the world. the h ... | 2010 | 19859704 |
| test characteristics of the sd fk80 plasmodium falciparum/plasmodium vivax malaria rapid diagnostic test in a non-endemic setting. | the sd fk80 p.f/p.v malaria antigen rapid test (standard diagnostics, korea) (fk80) is a three-band malaria rapid diagnostic test detecting plasmodium falciparum histidine-rich protein-2 (hrp-2) and plasmodium vivax-specific lactate dehydrogenase (pv-pldh). the present study assessed its performance in a non-endemic setting. | 2009 | 19930609 |
| evaluation of the palutop+4 malaria rapid diagnostic test in a non-endemic setting. | palutop+4 (all. diag, strasbourg, france), a four-band malaria rapid diagnostic test (malaria rdt) targeting the histidine-rich protein 2 (hrp-2), plasmodium vivax-specific parasite lactate dehydrogenase (pv-pldh) and pan plasmodium-specific pldh (pan-pldh) was evaluated in a non-endemic setting on stored whole blood samples from international travellers suspected of malaria. | 2009 | 20003378 |
| [epidemiological baseline situation before the construction of a small dam in five villages of bouaké, central côte-d'ivoire]. | abstract from june 2007 to june 2008, entomological, malacological and parasitological investigations were carried out in five villages in close proximity to a small dam of raf-fierkro in bouaké, central côte-d'ivoire. the objective of the study was to identify vectors and intermediate host snails of parasitic diseases related to water, and to assess the prevalence of malaria, schistosomiasis and soil-transmitted helminthiasis before dam construction. mosquitoes were caught by human landing catc ... | 2010 | 20084484 |
| computational analysis of binding between malarial dihydrofolate reductases and anti-folates. | plasmodium falciparum readily develops resistance to the anti-folates pyrimethamine and proguanil via a characteristic set of mutations in the dihydrofolate reductase (pfdhfr) gene that leads to reduced competitive drug binding at the enzyme's active site. analogous mutations can be found in the dhfr gene in isolates of plasmodium vivax (pvdhfr) although anti-folates have not been widely used for the treatment of this infection. here the interactions between dhfr inhibitors and modelled structur ... | 2010 | 20193090 |
| [not available]. | 1947 | 20270434 | |
| multiplex real-time pcr for the diagnosis of malaria: correlation with microscopy. | malaria is generally diagnosed by microscopy and rapid antigen testing. molecular methods become more widely used. in the present study, the contribution of a quantitative multiplex malaria pcr was investigated. we assessed: (i) the agreement between pcr-based identification and microscopy and (ii) the correlation between the parasite load as determined by quantitative pcr and by microscopy. for 83 patients positive by microscopy for plasmodium spp., the first edta-blood sample was tested by mul ... | 2011 | 20298268 |
| [imported malaria in tunisia: consequences on the risk of resurgence of the disease]. | although malaria has been eradicated in tunisia since 1979, the disease is still a health issue due to the persistence of mosquitoes and coexistence with a potential parasite reservoir in the form of imported cases. from 1999 to 2006, 98 cases of imported malaria were diagnosed at the pasteur institute in tunis where nearly 30% of national cases are recorded. tunisians accounted for 24.5% of these cases versus 75.5% involving foreigners. the occurrence rate has steadily increased in volunteer wo ... | 2010 | 20337112 |
| two nonrecombining sympatric forms of the human malaria parasite plasmodium ovale occur globally. | malaria in humans is caused by apicomplexan parasites belonging to 5 species of the genus plasmodium. infections with plasmodium ovale are widely distributed but rarely investigated, and the resulting burden of disease is not known. dimorphism in defined genes has led to p. ovale parasites being divided into classic and variant types. we hypothesized that these dimorphs represent distinct parasite species. | 2010 | 20380562 |
| human malaria parasites: are we ready for a new species? | 2010 | 20380563 | |
| sub-microscopic malaria cases and mixed malaria infection in a remote area of high malaria endemicity in rattanakiri province, cambodia: implication for malaria elimination. | malaria microscopy and rapid diagnostic tests are insensitive for very low-density parasitaemia. this insensitivity may lead to missed asymptomatic sub-microscopic parasitaemia, a potential reservoir for infection. similarly, mixed infections and interactions between plasmodium species may be missed. the objectives were first to develop a rapid and sensitive pcr-based diagnostic method to detect low parasitaemia and mixed infections, and then to investigate the epidemiological importance of sub- ... | 2010 | 20409349 |
| malaria infection and human evolution. | during the evolution of the genus homo, with regard to the species habilis, erectus and sapiens, malaria has played a key biological role in influencing human development. the plasmodia causing malaria have evolved in two ways, in biological and phylogenetic terms: plasmodium vivax, plasmodium malariae and plasmodium ovale appear to have either coevolved with human mankind, or encountered human species during the most ancient phases of homo evolution; on the other hand, plasmodium falciparum has ... | 2010 | 20424529 |
| african apes as reservoirs of plasmodium falciparum and the origin and diversification of the laverania subgenus. | we investigated two mitochondrial genes (cytb and cox1), one plastid gene (tufa), and one nuclear gene (ldh) in blood samples from 12 chimpanzees and two gorillas from cameroon and one lemur from madagascar. one gorilla sample is related to plasmodium falciparum, thus confirming the recently reported presence in gorillas of this parasite. the second gorilla sample is more similar to the recently defined plasmodium gaboni than to the p. falciparum-plasmodium reichenowi clade, but distinct from bo ... | 2010 | 20498054 |
| isolation and characterization of the msp1 genes from plasmodium malariae and plasmodium ovale. | the merozoite surface protein 1 (msp1) is the principal surface antigen of the blood stage form of the plasmodium parasite. antibodies recognizing msp1 are frequently detected following plasmodium infection, making this protein a significant component of malaria vaccines and diagnostic tests. although the msp1 gene sequence has been reported for plasmodium falciparum and plasmodium vivax, this gene has not been identified for the other two major human-infectious species, plasmodium malariae and ... | 2010 | 20519591 |
| evaluation of a rapid diagnostic test (carestart malaria hrp-2/pldh (pf/pan) combo test) for the diagnosis of malaria in a reference setting. | malaria rapid diagnostic tests (rdts) are widely used for diagnosing malaria. the present retrospective study evaluated the carestart malaria hrp-2/pldh (pf/pan) combo test targeting the plasmodium falciparum specific antigen histidine-rich protein (hrp-2) and the pan-plasmodium antigen lactate dehydrogenase (pldh) in a reference setting. | 2010 | 20565816 |
| indigenous plasmodium ovale malaria in bangladesh. | in spite of the high prevalence of malaria in southeastern bangladesh, there remains a significant shortage of information regarding the presence of three of five human malaria parasites: plasmodium ovale, p. malariae, and p. knowlesi. the presence of p. ovale and p. knowlesi has previously never been reported from bangladesh. we used a genus- and species-specific nested polymerase chain reaction, targeting highly conserved regions of the small subunit ribosomal rna (ssu rrna) gene, to investiga ... | 2010 | 20595481 |
| malaria: origin of the term "hypnozoite" | the term "hypnozoite" is derived from the greek words hypnos (sleep) and zoon (animal). hypnozoites are dormant forms in the life cycles of certain parasitic protozoa that belong to the phylum apicomplexa (sporozoa) and are best known for their probable association with latency and relapse in human malarial infections caused by plasmodium ovale and p. vivax. consequently, the hypnozoite is of great biological and medical significance. this, in turn, makes the origin of the name "hypnozoite" a su ... | 2010 | 20665090 |
| detection of plasmodium falciparum, p. vivax, p. ovale, and p. malariae merozoite surface protein 1-p19 antibodies in human malaria patients and experimentally infected nonhuman primates. | approximately 3.2 billion people live in areas where malaria is endemic, and who estimates that 350 to 500 million malaria cases occur each year worldwide. this high prevalence, and the high frequency of international travel, creates significant risk for the exportation of malaria to countries where malaria is not endemic and for the introduction of malaria organisms into the blood supply. since all four human infectious plasmodium species have been transmitted by blood transfusion, we sought to ... | 2010 | 20702658 |
| validation of a four-primer real-time pcr as a diagnostic tool for single and mixed plasmodium infections. | clin microbiol infect abstract: although microscopy remains the reference standard for malaria diagnosis, molecular tools are attracting increasing interest. to improve the detection of mixed infections, we developed a four-primer real-time pcr with four plasmodium species-specific forward primers, based on the pan-primer design with universal plasmodium primers as described previously. after validation for analytical sensitivity, specificity and reproducibility, the four-primer pcr was evaluate ... | 2010 | 20718798 |
| a case of malaria due to plasmodium ovale stephens 1922. | 1933 | 20777666 | |
| plasmodium ovale infection in africa. | 1937 | 20780443 | |
| persistence of plasmodium ovale. | 1945 | 20785964 | |
| pre-erythrocytic stages of human malaria: plasmodium ovale. | 1954 | 20788195 | |
| what is the evidence for the existence of plasmodium ovale hypnozoites? | relapsing human malaria is regarded to be caused by plasmodium vivax and plasmodium ovale. p. vivax relapses originate from dormant liver stages: "hypnozoites". also, p. ovale, a species considered as closely related to p. vivax, is in analogy assumed to display hypnozoites. a close biologic relationship is, however, not supported by molecular genetic studies. therefore, original literature published since the description of p. ovale in 1922 was systematically screened for the demonstration of h ... | 2010 | 20922429 |
| plasmodium ovale infection in malaysia: first imported case. | plasmodium ovale infection is rarely reported in malaysia. this is the first imported case of p. ovale infection in malaysia which was initially misdiagnosed as plasmodium vivax. | 2010 | 20929588 |
| in vivo and in vitro efficacy of chloroquine against plasmodium malariae and p. ovale in papua, indonesia. | reports of potential drug-resistant strains of plasmodium malariae in western indonesia raise concerns that chloroquine resistance may be emerging in p. malariae and p. ovale. in order to assess this, in vivo and in vitro efficacy studies were conducted in patients with monoinfection in papua, indonesia. consecutive patients with uncomplicated malaria due to p. ovale or p. malariae were enrolled in a prospective clinical trial, provided with supervised chloroquine treatment, and followed for 28 ... | 2010 | 20937779 |
| splenic infarction during plasmodium ovale acute malaria: first case reported. | the splenic complications of acute malaria include two different prognostic and treatment entities: splenic infarction and splenic rupture. this is the first case of splenic infarction during an acute malaria due to plasmodium ovale in a 34-year-old man. as in the majority other described cases of splenic infarction, the course was spontaneously favourable, suggesting that this complication was relatively benign compared to splenic rupture, which is life-threatening and usually necessitating sur ... | 2010 | 20955610 |
| transfusion-related plasmodium ovale malaria complicated by acute respiratory distress syndrome (ards) in a non-endemic country. | 46year old female presented with a one week history of high grade fever, chills, cough, and severe nausea. the patient had been admitted a month earlier with severe lower gastrointestinal bleeding from hemorrhoids necessitating transfusion of 7 units of packed red blood cells. initial work-up was unremarkable. because of persistent symptoms, the patient was admitted 2 days later. malaria smear was positive. due to the severity of her symptoms, she was managed as falciparum malaria and was starte ... | 2010 | 20971212 |
| five-minute giemsa stain for rapid detection of malaria parasites in blood smears. | the giemsa stain is used as the gold standard for the diagnosis of malaria on blood smears. the classical staining procedure requires between 30 and 45 min. we modified the giemsa stain and reduced the staining time to 5 min without any loss of quality. | 2010 | 21088023 |
| a taqman real-time pcr assay for the detection and quantitation of plasmodium knowlesi. | the misdiagnosis of plasmodium knowlesi by microscopy has prompted a re-evaluation of the geographic distribution, prevalence and pathogenesis of this species using molecular diagnostic tools. in this report, a specific probe for p. knowlesi, that can be used in a previously described taqman real-time pcr assay for detection of plasmodium spp., and plasmodium falciparum, plasmodium vivax, plasmodium malariae and plasmodium ovale, was designed and validated against clinical samples. | 2010 | 21114872 |
| the challenge of diagnosing plasmodium ovale malaria in travellers: report of six clustered cases in french soldiers returning from west africa. | plasmodium ovale is responsible for 5% of imported malaria in french travellers. the clinical and biological features of six clustered cases of p. ovale malaria in an army unit of 62 french soldiers returning from the ivory coast are reported. | 2010 | 21143962 |
| high-throughput molecular diagnosis of circumsporozoite variants vk210 and vk247 detects complex plasmodium vivax infections in malaria endemic populations in papua new guinea. | malaria is endemic in lowland and coastal regions of papua new guinea (png), and is caused by plasmodium falciparum, plasmodium vivax, plasmodium malariae and plasmodium ovale. infection by p. vivax is attributed to distinct strains, vk210 and vk247, which differ in the sequence of the circumsporozoite protein (pvcsp). here, based upon sequence polymorphisms in pvcsp, we developed a post-pcr ligation detection reaction-fluorescent microsphere assay (ldr-fma) to distinguish these p. vivax strains ... | 2010 | 21147267 |
| malaria transmission in two rural communities in the forest zone of ghana. | malaria transmission was assessed in two rural communities, kona and afamanaso in sekyere south district, ashanti region, in the forest zone of ghana to provide baseline data for ongoing clinical studies and the evaluation of the effect of interventions. altogether, 3,479 anopheles gambiae and 1,157 anopheles funestus were caught by human landing catches. sporozoite rates determined by either microscopy of salivary glands or enzyme-linked immunosorbent assay (elisa) for plasmodium falciparum in ... | 2010 | 21153839 |
| giemsa-stained thick blood films as a source of dna for plasmodium species-specific real-time pcr. | this study describes the use of thick blood films (tbf) as specimens for dna amplification with the plasmodium species-specific real-time pcr that was recently validated on whole blood samples. | 2010 | 21176207 |
| [plasmodium ovale malaria severity]. | 2010 | 21194860 | |
| evaluation of the rapid diagnostic test sdfk40 (pf-pldh/pan-pldh) for the diagnosis of malaria in a non-endemic setting. | the present study evaluated the sd bioline malaria ag 05fk40 (sdfk40), a three-band rdt detecting plasmodium falciparum-specific parasite lactate dehydrogenase (pf-pldh) and pan plasmodium-specific pldh (pan-pldh), in a reference setting. | 2011 | 21226920 |
| variant plasmodium ovale isolated from a patient infected in ghana. | recent data have found that plasmodium ovale can be separated in two distinct species: classic and variant p. ovale based on multilocus typing of different genes. this study presents a p. ovale isolate from a patient infected in ghana together with an analysis of the small subunit rna, cytochrome b, cytochrome c oxidase i, cysteine protease and lactate dehydrogenase genes, which show that the sample is a variant p. ovale and identical or highly similar to variant p. ovale isolated from humans in ... | 2011 | 21255451 |
| plasmodium ovale curtisi and plasmodium ovale wallikeri circulate simultaneously in african communities. | it has been proposed that ovale malaria in humans is caused by two closely related but distinct species of malaria parasite, plasmodium ovale curtisi and plasmodium ovale wallikeri. it was recently shown that these two parasite types are sympatric at the country level. however, it remains possible that localised geographic, temporal or ecological barriers exist within endemic countries which prevent recombination between the genomes of the two species. here, using conventional and real-time quan ... | 2011 | 21315074 |
| [plasmodium ovale infection: description of 16 cases and a review]. | information on imported malaria caused by plasmodium ovale parasite is scarce. | 2011 | 21342729 |
| rapid diagnostic tests as a source of dna for plasmodium species-specific real-time pcr. | this study describes the use of malaria rapid diagnostic tests (rdts) as a source of dna for plasmodium species-specific real-time pcr. | 2011 | 21435256 |
| continuing intense malaria transmission in northern uganda. | abstract. recent reports of reductions in malaria transmission in several african countries have resulted in optimism that malaria can be eliminated in parts of africa where it is currently endemic. it is not known whether these trends are global or whether they are also present in areas where political instability has hindered effective malaria control. we determined malaria parasite carriage and age-dependent antibody responses to plasmodium falciparum antigens in cross-sectional surveys in ap ... | 2011 | 21540398 |
| [acute pericarditis associated with plasmodium ovale malaria]. | the purpose of this report is to describe a rare case of benign acute pericarditis associated with recurrent plasmodium ovale malaria. it was observed in a 33-year-old soldier who was stationed in djibouti after serving several previous stints in west africa. a favorable clinical outcome was achieved using chloroquin (30 mg/kg on 6 days) in association with nsaid followed by salicylates for one month. re-examination at one year showed no recurrence. this case shows that plasmodium ovale malaria ... | 2011 | 21585100 |
| rodent blood-stage plasmodium survive in dendritic cells that infect naive mice. | plasmodium spp. parasites cause malaria in 300 to 500 million individuals each year. disease occurs during the blood-stage of the parasite's life cycle, where the parasite is thought to replicate exclusively within erythrocytes. infected individuals can also suffer relapses after several years, from plasmodium vivax and plasmodium ovale surviving in hepatocytes. plasmodium falciparum and plasmodium malariae can also persist after the original bout of infection has apparently cleared in the blood ... | 2011 | 21690346 |
| detection of high levels of mutations involved in anti-malarial drug resistance in plasmodium falciparum and plasmodium vivax at a rural hospital in southern ethiopia. | abstract: background: in ethiopia, malaria is caused by plasmodium falciparum and plasmodium vivax, and anti-malarial drug resistance is the most pressing problem confronting control of the disease. since co-infection by both species of parasite is common and sulphadoxine-pyrimethamine (sp) has been intensively used, resistance to these drugs has appeared in both p. falciparum and p. vivax populations. this study was conducted to assess the prevalence of anti-malarial drug resistance in p. falci ... | 2011 | 21810256 |
| [A history of malaria in modern Korea 1876-1945]. | Although it is not certain when malaria began to appear in Korea, malaria is believed to have been an endemic disease from ancient times. It was Dr. H. N. Allen (1858-1932) who made the first description and diagnosis of malaria in terms of Western medicine. In his first year report (1885) of Korean Government Hospital he mentioned malaria as the most prevalent disease. Very effective anti-malarial drug quinine was imported and it made great contribution in treating malaria. After Japan had anne ... | 2011 | 21894070 |
| malaria rapid diagnostic tests in elimination settings--can they find the last parasite? | rapid diagnostic tests (rdts) for malaria have improved the availability of parasite-based diagnosis throughout the malaria-endemic world. accurate malaria diagnosis is essential for malaria case management, surveillance, and elimination. rdts are inexpensive, simple to perform, and provide results in 15-20 min. despite high sensitivity and specificity for plasmodium falciparum infections, rdts have several limitations that may reduce their utility in low-transmission settings: they do not relia ... | 2011 | 21910780 |
| Evaluation of the Clearview® Malaria pLDH Malaria Rapid Diagnostic Test in a non-endemic setting. | Malaria Rapid Diagnostic Tests (RDTs) are widely used to diagnose malaria. The present study evaluated a new RDT, the Clearview® Malaria pLDH test targeting the pan-Plasmodium antigen lactate dehydrogenase (pLDH). | 2011 | 21951996 |
| Diagnosis and therapy for hospitalized imported malaria in adults in Italy. | The diagnosis and treatment of malaria in non-endemic countries presents a continuing challenge. | 2011 | 22017713 |
| Prevalence of Malaria among Patients Attending Public Health Facilities in Maputo City, Mozambique. | Abstract. We conducted a health facility-based survey to estimate the prevalence of malaria among febrile patients at health facilities (HFs) in Maputo City. Patients answered a questionnaire on malaria risk factors and underwent malaria testing. A malaria case was defined as a positive result for malaria by microscopy in a patient with fever or history of fever in the previous 24 hours. Among 706 patients with complete information, 111 (15.7%) cases were identified: 105 were positive for Plasmo ... | 2011 | 22144434 |
| cloning and heterologous expression of plasmodium ovale dihydrofolate reductase-thymidylate synthase gene. | plasmodial bifunctional dihydrofolate reductase-thymidylate synthase (dhfr-ts) is a validated antimalarial drug target. in this study, expression of the putative dhfr-ts of plasmodium ovale rescued the dhfr chemical knockout and a ts null bacterial strain, demonstrating its dhfr and ts catalytic functions. podhfr-ts was expressed in escherichia coli bl21 (de3) and affinity purified by methotrexate sepharose column. biochemical and enzyme kinetics characterizations indicated that podhfr-ts is sim ... | 2012 | 22234170 |
| human plasmodium knowlesi infection in ranong province, southwestern border of thailand. | plasmodium knowlesi, a simian malaria parasite, has been reported in humans in many southeast asian countries. in thailand, most of the limited numbers of cases reported so far were from areas near neighbouring countries, including myanmar. | 2012 | 22313518 |
| 'not another uti ... '. | malaria includes a global disease burden with approximately 300-500 million cases worldwide annually. varied symptomatology creates a diagnostic challenge. this is a case report of a stroke rehabilitation facility resident who developed plasmodium ovale infection, several months post-exposure. physicians should maintain a broad list of differential diagnoses, thinking beyond the range of common diagnoses. | 2012 | 22417982 |
| plasmodium knowlesi: from malaysia, a novel health care threat. | epidemic foci of plasmodium knowlesi malaria have been identified during the past ten years in malaysia, in particular in the states of sarawak and sabah (malaysia borneo), and in the pahang region (peninsular malaysia). based on a review of the available recent international literature, the authors underline the importance of molecular biology examinations, polymerase chain reactions (pcr), performed with primers specific for p. knowlesi, since the current microscopic examination (haemoscope) m ... | 2012 | 22475654 |
| prospective evaluation of artemether-lumefantrine for the treatment of non-falciparum and mixed-species malaria in gabon. | the recommendation of artemisinin combination therapy (act) as first-line treatment for uncomplicated falciparum malaria is supported by a plethora of high quality clinical trials. however, their recommendation for the treatment of mixed-species malaria and the large-scale use for the treatment of non-falciparum malaria in endemic regions is based on anecdotal rather than systematic clinical evidence. | 2012 | 22515681 |
| host immune response in returning travellers infected with malaria. | clinical observations suggest that canadian-born (cb) travellers are prone to more severe malaria, characterized by higher parasite density in the blood, and severe symptoms, such as cerebral malaria and renal failure, than foreign-born travellers (fb) from areas of malaria endemicity. it was hypothesized that host cytokine and chemokine responses differ significantly in cb versus fb patients returning with malaria, contributing to the courses of severity. a more detailed understanding of the pr ... | 2012 | 22554058 |
| plasmodium ovale in bangladesh: genetic diversity and the first known evidence of the sympatric distribution of plasmodium ovale curtisi and plasmodium ovale wallikeri in southern asia. | in spite of the high prevalence of malaria in bangladesh and other southern asian countries, there remains a substantial shortage of knowledge about the less common human malaria parasites. recent studies indicate that plasmodium ovale is made up of two species, namely plasmodium ovale wallikeri and plasmodium ovale curtisi. genus- and species-specific nested pcr analyses of the ssrrna gene was used to detect p. ovale infections among 2,246 diagnostic samples. plasmodium ovale infections were fu ... | 2012 | 22633951 |
| cloning, overexpression, purification and characterization of plasmodium knowlesi lactate dehydrogenase. | plasmodial lactate dehydrogenase, key enzyme of anaerobic glycolysis, has been shown to be a potential immunodiagnostic marker as well as a novel target for chemotherapy. we have cloned, overexpressed and immunochemically characterized the recombinant lactate dehydrogenase of plasmodium knowlesi, the fifth human malaria parasite. the p. knowlesi lactate dehydrogenase (pkldh) gene was pcr amplified and 0.9 kb pcr product was cloned into pgem-t easy vector. sequencing and blast analysis revealed o ... | 2012 | 22683723 |
| evaluation of the rapid diagnostic test carestart pldh malaria (pf-pldh/pan-pldh) for the diagnosis of malaria in a reference setting. | the present study evaluated carestart pldh malaria, a three-band rapid diagnostic test detecting plasmodium falciparum-specific parasite lactate dehydrogenase (pf-pldh) and pan plasmodium-specific pldh (pan-pldh) in a reference setting. | 2012 | 22704733 |
| the first case of plasmodium ovale malaria from bangladesh. | the authors report the first indigenous case of plasmodium ovale infection from bangladesh. the diagnosis was confirmed by pcr and sequence analysis. the patient had neither been outside of the country nor ever received blood transfusions. the authors concluded that there was evidence for a local transmission of p ovale malaria in bangladesh. p ovale malaria should therefore always be considered a potential differential diagnosis in the indigenous population as well as travellers and migrants re ... | 2010 | 22778371 |
| cyp450 phenotyping and accurate mass identification of metabolites of the 8-aminoquinoline, anti-malarial drug primaquine. | the 8-aminoquinoline (8aq) drug primaquine (pq) is currently the only approved drug effective against the persistent liver stage of the hypnozoite forming strains plasmodium vivax and plasmodium ovale as well as stage v gametocytes of plasmodium falciparum. to date, several groups have investigated the toxicity observed in the 8aq class, however, exact mechanisms and/or metabolic species responsible for pq's haemotoxic and anti-malarial properties are not fully understood. | 2012 | 22856549 |
| real-time pcr assay for discrimination of plasmodium ovale curtisi and plasmodium ovale wallikeri in the ivory coast and in the comoros islands. | plasmodium ovale is one of the five malaria species infecting humans. recent data have shown that the name of this neglected species masks two distinct genotypes also called curtisi and wallikeri. some authors show that these species could be sympatric. these two subspecies are not differentiated by microscopy techniques and malaria rapid diagnostic tests. this diagnostic defect is the result of low parasitaemia, antigenic polymorphism and absence of antibodies performance and requires the use o ... | 2012 | 22947418 |
| new treatment policy of malaria as a part of malaria control program in indonesia. | malaria control program is one of the oldest program in the ministry of health (moh) republic of indonesia. started with effort to eradicate malaria in 1959 through malaria eradication command well known as kopem (komando pembasmian malaria) then it evolves to malaria control program, roll back malaria program, and the current malaria elimination program. in terms of diagnostic and treatment, the policy has formulated by strictly follow evidence-based principles as well as technical guided from ... | 2012 | 22983085 |
| management of imported malaria in europe. | in this position paper, the european society for clinical microbiology and infectious diseases, study group on clinical parasitology, summarizes main issues regarding the management of imported malaria cases. malaria is a rare diagnosis in europe, but it is a medical emergency. a travel history is the key to suspecting malaria and is mandatory in patients with fever. there are no specific clinical signs or symptoms of malaria although fever is seen in almost all non-immune patients. migrants fro ... | 2012 | 22985344 |
| two techniques for simultaneous identification of plasmodium ovale curtisi and plasmodium ovale wallikeri by use of the small-subunit rrna gene. | the primers traditionally used to detect plasmodium ovale infections are known for not binding all p. ovale parasites within the small-subunit rrna gene when used alone. we describe a simple, cost- and time-efficient multiplex nested pcr and a nested pcr using a novel set of primers for the simultaneous detection of p. ovale curtisi and p. ovale wallikeri. | 2012 | 23015675 |
| hexaplex pcr detection system for identification of five human plasmodium species with an internal control. | malaria remains one of the major killers of humankind and persists to threaten the lives of more than one-third of the world's population. given that human malaria can now be caused by five species of plasmodium, i.e., plasmodium falciparum, plasmodium vivax, plasmodium malariae, plasmodium ovale, and the recently included plasmodium knowlesi, there is a critical need not only to augment global health efforts in malaria control but also, more importantly, to develop a rapid, accurate, species-se ... | 2012 | 23035191 |
| [the vivax malaria causative agent plasmodium ovale: the present global area, intraspecies polymorphism, importation to the russian federation (1992-2011)]. | the global area for plasmodium ovale is small as compared with that for other species of human malaria pathogens. it has expanded in asian areas and remained as before in the african ones. in the past 20 years, there have been 2129 malaria cases imported from far abroad to russia, including 84 (4%) cases of vivax malaria (p. ovale). the patients were most foreign citizens: 70 from 20 african countries and 7 from two countries of oceania, such as papua new guinea and indonesia. the other 7 patien ... | 2012 | 23088152 |
| a new real-time pcr for the detection of plasmodium ovale wallikeri. | it has been proposed that ovale malaria in humans is caused by two closely related but distinct species of malaria parasites: p. ovale curtisi and p. ovale wallikeri. we have extended and optimized a real-time pcr assay targeting the parasite's small subunit ribosomal rna (ssrrna) gene to detect both these species. when the assay was applied to 31 archival blood samples from patients diagnosed with p. ovale, it was found that the infection in 20 was due to p. ovale curtisi and in the remaining 1 ... | 2012 | 23110165 |
| discordance in drug resistance-associated mutation patterns in marker genes of plasmodium falciparum and plasmodium knowlesi during coinfections. | human plasmodium knowlesi infections have been reported from several south-east asian countries, excluding india, but its drug susceptibility profile in mixed-infection cases remains unknown. | 2013 | 23292346 |
| self-diagnosis of malaria by travelers and expatriates: assessment of malaria rapid diagnostic tests available on the internet. | in the past malaria rapid diagnostic tests (rdts) for self-diagnosis by travelers were considered suboptimal due to poor performance. nowadays rdts for self-diagnosis are marketed and available through the internet. the present study assessed rdt products marketed for self-diagnosis for diagnostic accuracy and quality of labeling, content and instructions for use (ifu). | 2013 | 23301027 |
| a novel, single-amplification pcr targeting mitochondrial genome highly sensitive and specific in diagnosing malaria among returned travellers in bergen, norway. | nested pcr is a commonly used technique in diagnosis of malaria owing to its high sensitivity and specificity. however, it is time-consuming, open to considerable risk of contamination and has low cost-efficiency. using amplification targets presented in multiple copies, such as rrna 18s, or mitochondrial targets with an even higher copy number, might increase sensitivity. | 2013 | 23336125 |
| malaria rapid diagnostic tests in travel medicine. | malaria is a serious condition in the non-immune traveller, and prognosis depends on timely diagnosis. although microscopy remains the cornerstone of diagnosis, malaria rapid diagnostic tests (rdts) are increasingly used in non-endemic settings. they are easy to use, provide results rapidly and require no specific training and equipment. reported sensitivities vary between different rdt products but are generally good for plasmodium falciparum, with rdts detecting the p. falciparum antigen histi ... | 2013 | 23373854 |
| plasmodium species occurrence, temporal distribution and interaction in a child-aged population in rural burkina faso. | malaria can be caused by five plasmodium species. due to their higher prevalence, much of the research concentrates on plasmodium falciparum and plasmodium vivax. in burkina faso, where p. falciparum co-exists with plasmodium malariae and plasmodium ovale, there is not much data about the prevalence of the latter two species across human population. moreover, interactions between co-infecting plasmodium species are not documented. the aim of the current research is to determine species-specific ... | 2013 | 23421809 |
| malaria rapid diagnostic tests in endemic settings. | malaria rapid diagnostic tests (rdts) are instrument-free tests that provide results within 20 min and can be used by community health workers. rdts detect antigens produced by the plasmodium parasite such as plasmodium falciparum histidine-rich protein-2 (pfhpr2) and plasmodium lactate dehydrogenase (pldh). the accuracy of rdts for the diagnosis of uncomplicated p. falciparum infection is equal or superior to routine microscopy (but inferior to expert microscopy). sensitivity for plasmodium viv ... | 2013 | 23438048 |