Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [application of rapid pcr to authenticate medicinal snakes]. | to obtained an accurate, rapid and efficient method for authenticate medicinal snakes listed in chinese pharmacopoeia (zaocysd humnades, bungarus multicinctus, agkistrodon acutus), a rapid pcr method for authenticate snakes and its adulterants was established based on the classic molecular authentication methods. dna was extracted by alkaline lysis and the specific primers were amplified by two-steps pcr amplification method. the denatured and annealing temperature and cycle numbers were optimiz ... | 2014 | 25612419 |
| a novel direct factor xa inhibitory peptide with anti-platelet aggregation activity from agkistrodon acutus venom hydrolysates. | snake venom is a natural substance that contains numerous bioactive proteins and peptides, nearly all of which have been identified over the last several decades. in this study, we subjected snake venom to enzymatic hydrolysis to identify previously unreported bioactive peptides. the novel peptide ach-11 with the sequence ltfprivfvlg was identified with both fxa inhibition and anti-platelet aggregation activities. ach-11 inhibited the catalytic function of fxa towards its substrate s-2222 via a ... | 2015 | 26035670 |
| envenoming by crotalid snake chinese moccasin agkistrodon acutus bite - a case report. | although the bites caused by snakes from former agkistrodon family in the areas of occurrence are not rare and even have certain epidemiologic importance, in case of envenoming by deinagkistrodon acutus the clinical studies and case reports are very sporadic. this case report describes the envenoming of a private snake breeder bitten by young chinese moccasin deinagkistrodon acutus to the thumb of his left hand. he sought for a medical help immediately after snakebite. patient presented with a l ... | 2015 | 26093669 |
| balancing the expression and production of a heterodimeric protein: recombinant agkisacutacin as a novel antithrombotic drug candidate. | agkisacucetin extracted from the venom of agkistrodon acutus has been demonstrated to be a promising antithrombotic drug candidate in clinical studies due to its function as a novel platelet membrane glycoprotein (gp) ib inhibitor. agkisacucetin is a heterodimeric protein composed of α- and β-subunits with seven disulphide bonds. both subunits form inactive homodimeric products, which cause difficulties for recombinant production. in this study, agkisacucetin α- and β-subunits were inserted sequ ... | 2015 | 26144864 |
| design, synthesis of novel tryptophan derivatives for antiplatelet aggregation activity based on tripeptide penw (pglu-asn-trp). | penw, a three mer peptide derived from agkistrodon acutus guenther venom, has been found to be an antagonist of the gpiib/iiia receptor and shows antiplatelet aggregation activity. based on penw and a gpiib/iiia inhibitor tirofiban, a series of tryptophan derivatives were designed, synthesized and evaluated for their antiplatelet aggregation activity induced by adp. the most potent compound 87 was also tested for the bleeding time and antithrombotic activity in vivo in comparison with tirofiban. ... | 2015 | 26298494 |
| a novel fibrinogenase from agkistrodon acutus venom protects against lps-induced endotoxemia via regulating nf-κb pathway. | endotoxins including lipopolysaccharide (lps) could cause endotoxemia which often results in excessive inflammation, organ dysfunction, sepsis, disseminated intravascular coagulation (dic) or even death. previously, a novel fibrinogenase (fii) showed protective effects on lps-induced dic via activating protein c and suppressing inflammatory cytokines. | 2015 | 26369367 |
| antibodies against venom of the snake deinagkistrodon acutus. | snake venom protein from deinagkistrodon acutus (da protein), one of the major venomous species in taiwan, causes hemorrhagic symptoms that can lead to death. although horse-derived antivenin is a major treatment, relatively strong and detrimental side effects are seen occasionally. in our study, yolk immunoglobulin (igy) was purified from eggs, and da protein was recognized using western blotting and an enzyme-linked immunosorbent assay (elisa), similar to therapeutic horse antivenin. the elisa ... | 2015 | 26475102 |
| antiplatelet aggregation and antithrombosis efficiency of peptides in the snake venom of deinagkistrodon acutus: isolation, identification, and evaluation. | two peptides of pt-a (glu-asn-trp 429 da) and pt-b (glu-gln-trp 443 da) were isolated from venom liquor of deinagkistrodon acutus. their antiplatelet aggregation effects were evaluated with platelet-rich human plasma in vitro; the respective ic50 of pt-a and pt-b was 66 μm and 203 μm. both peptides exhibited protection effects on adp-induced paralysis in mice. after adp administration, the paralysis time of different concentration of pt-a and pt-b lasted as the following: 80 mg/kg pt-b (152.8 ± ... | 2015 | 26483843 |
| homogeneous fluorescent specific pcr for the authentication of medicinal snakes using cationic conjugated polymers. | a label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid pcr technique with water-soluble cationic conjugated polyelectrolytes (ccps). three medicinal snake materials (deinagkistrodon acutus, zaocys dhumnades and bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a ccp-based assay in a high-t ... | 2015 | 26537289 |
| [effect of aavc-i on the migration of human umbilical vein endothelial cells]. | to investigate the effect of component i from agkistrodon acutus venomon (aavc-i) the migration of human umbilical vein endothelial cells (huvecs), and to elucidate the possible anti-angiogenic mechanism of aavc-i. | 2015 | 26827530 |
| bioinformatics-based design of novel antigenic b-cell linear epitopes of deinagkistrodon acutus venom. | with the help of bioinformatics analysis, we wished to develop a novel antivenom against the deinagkistrodon (d.) acutus snake venom using b-cell linear epitopes of three primary toxins (serine protease, metalloprotease, and phospholipase a2). | 2016 | 26957285 |
| purification and immunoprotection evaluation of aahiv from complex venom metalloproteinases of deinagkistrodon acutus. | the aim of this study was to investigate the immunoprotective effects of aahiv in mice. after purification, a 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was performed. bicinchoninic acid was used to determine the molecular weight and concentration of aahiv. aahiv, venom complex (vc), and phosphate buffered saline (pbs) were subsequently used to immunize the mice three times, and the blood was sampled 1 week after the third immunization to determine the serum immunog ... | 2016 | 27111246 |
| screening, tandem expression and immune activity appraisal of deinagkistrodon acutus (pit viper) venom mimotopes from a phage display 12-mer peptide library. | to design a specific polyclonal antibody against deinagkistrodon acutus venom (da-pab) by immunizating new zealand white rabbits. | 2016 | 27421965 |
| agkistrodon acutus-purified protein c activator protects human umbilical vein endothelial cells against h2o2-induced apoptosis. | recent studies show that the agkistrodon acutus (viperidae) (syn. deinagkistrodon acutus) protein c activator (pca) treats acute myocardial infarction and ischaemia-reperfusion animal models effectively, while the underlying mechanism remains unknown. | 2016 | 27572701 |
| isolation and biochemical characterization of a gamma-type phospholipase a2inhibitor from macropisthodon rudis snake serum. | a novel phospholipasea2(pla2) inhibitory protein (pli) was purified from the serum of macropisthodon rudis, a non-venomous snake mainly found in southern china. the molecular mass of the purified pli was 160 kda as determined by superdex 200hr; however, the pli protein had only one subunit of 25.4 kda as determined by 12% sds-page, indicating an oligomeric protein. pli cdna obtained by pcr from the liver of macropisthodon rudis, revealed 549 bps coding for a mature protein of 183 amino acid resi ... | 2016 | 27641751 |
| evolutionary trajectories of snake genes and genomes revealed by comparative analyses of five-pacer viper. | snakes have numerous features distinctive from other tetrapods and a rich history of genome evolution that is still obscure. here, we report the high-quality genome of the five-pacer viper, deinagkistrodon acutus, and comparative analyses with other representative snake and lizard genomes. we map the evolutionary trajectories of transposable elements (tes), developmental genes and sex chromosomes onto the snake phylogeny. tes exhibit dynamic lineage-specific expansion, and many viper tes show br ... | 2016 | 27708285 |
| the impact of hemocoagulase for improvement of coagulation and reduction of bleeding in fracture-related hip hemiarthroplasty geriatric patients: a prospective, single-blinded, randomized, controlled study. | uncontrolled bleeding is associated with poor outcomes and mortality in geriatric patients undergoing hemiarthroplasty. hemocoagulase agkistrodon is a hemocoagulative, anti-hemorrhagic enzyme complex from deinagkistrodon acutus snake venom. this study aimed to investigate the efficacy of hemocoagulase agkistrodon on coagulation and bleeding outcomes in fracture-related hemiarthroplasty. | 2017 | 28238301 |
| deinagkistrodon acutusenvenomation: a report of three cases. | deinagkistrodon acutusenvenomation is associated with severe hematological and wound complications but is rarely described. | 2017 | 28344596 |
| preparation and neutralization efficacy of igy antibodies raised againstdeinagkistrodon acutusvenom. | the five-paced pit viper (deinagkistrodon acutus), endemic to china and northern vietnam, is responsible for most snakebites in the chinese territory. antivenom produced from horses is the main treatment for snakebites, but it may cause numerous clinical side effects and have other disadvantages involved in their production such as the welfare of animals. the present study was conducted aiming to develop an alternative antibody (igy) from the egg yolk of leghorn chickens immunized with snake ven ... | 2017 | 28396683 |
| [effect of agkistrodon acutus venom pca on huvec activity]. | to investigate the effects of agkistrodon acutus venom protein c activator(pca) on ultrastructure of human umbilical vein endothelial cells(huvec), and the levels of tissue factor(tf), vascular von willebrand factor (vwf) and endothelin-1 secreted by huvec and to clarify the anti-thrombotic mechanism of pca. | 2017 | 28446313 |
| dacin, one metalloproteinase from deinagkistrodon acutus venom inhibiting contraction of mouse ileum muscle. | mice were bitten by five-pace vipers (deinagkistrodon acutus), and then envenomed. it was well-known that the snake venom mainly disturbed the blood homeostasis of the envenomed victims. ocassionally, we found that the venom of d. acutus could inhibit the contraction tension of mouse ileum, so in this study we aimed to identify the active component inhibiting the contraction tension of mouse ileum in the snake venom. | 2017 | 28701157 |
| investigation of the inhibitory potential of phospholipase a2inhibitor gamma from sinonatrix annularis to snake envenomation. | sapliγ is a novel gamma phospholipase a2inhibitor (pli) recently isolated from sinonatrix annularis, a chinese endemic non-venomous snake. to explore the neutralization effects of sapliγ in snakebite envenomation, a dose equivalent to ld50of deinagkistrodon acutus, agkistrodon halys and naja atra venom with/without sapliγ was inoculated into the gastrocnemius muscle of female kunming mice. the ability of sapliγ to inhibit myonecrosis and systemic toxicity were evaluated through investigations of ... | 2017 | 28746861 |
| cloning, expression, purification and bioactivity evaluation of a thrombin-like enzyme from deinagkistrodon acutus venom gland library. | to identify a new member of serine proteases from deinagkistrodon acutus via phage display technique and appraise its biocatalytic activities. | 2018 | 28936710 |
| ulnar artery pseudoaneurysm and compartment syndrome formation after snake bite to the left forearm. | to report the outcome of a patient who developed pseudoaneurysms and compartment syndrome after a deinagkistrodon acutus snakebite. | 2017 | 29124975 |
| increased efficacy of antivenom combined with hyperbaric oxygen ondeinagkistrodon acutusenvenomation in adult rats. | snakebites are a neglected threat to global human health with a high morbidity rate. the present study explored the efficacy of antivenom with hyperbaric oxygen (hbo) intervention on snakebites, which could provide the experimental basis for clinical adjuvant therapy. | 2018 | 29363648 |
| three-dimensional computed tomography reconstructive diagnosis of snakebite-induced cerebral infarction. | despite common injury caused by snakebite, snakebite-induced ischemic stroke is rare. we reported on a patient who incurred a large cerebral infarction after being bitten by a deinagkistrodon acutus, one of the most poisonous snakes in the southwestern of china. applying 3d computed tomography (ct) of head combined with cerebral angiography examinations showed a large cerebral infarction, hernia in the right brain, developmental abnormalities of the right middle cerebral artery and cerebral arte ... | 2018 | 29480239 |
| venom proteomics and antivenom neutralization for the chinese eastern russell's viper, daboia siamensis from guangxi and taiwan. | the eastern russell's viper (daboia siamensis) causes primarily hemotoxic envenomation. applying shotgun proteomic approach, the present study unveiled the protein complexity and geographical variation of eastern d. siamensis venoms originated from guangxi and taiwan. the snake venoms from the two geographical locales shared comparable expression of major proteins notwithstanding variability in their toxin proteoforms. more than 90% of total venom proteins belong to the toxin families of kunitz- ... | 2018 | 29867131 |
| effects of hemocoagulase agkistrodon on the coagulation factors and its procoagulant activities. | hemocoagulase agkistrodon (hca), a thrombin-like enzyme (tle) from the venom of the chinese moccasin snake (deinagkistrodon acutus), has been used in clinical practice as a hemostatic compound. the aim of this study was to further investigate the pharmacological properties of hca. | 2018 | 29872262 |
| proteomic characterization of six taiwanese snake venoms: identification of species-specific proteins and development of a siscapa-mrm assay for cobra venom factors. | deinagkistrodon acutus, trimeresurus stejnegeri, protobothrops mucrosquamatus, daboia russelii siamensis, bungarus multicinctus and naja atra are the six medically important venomous snake species in taiwan. in this study, we characterized and compared their venom protein profiles using proteomic approaches. the major snake venom proteins were identified by gelc-ms/ms and the total venom proteome was characterized by in-solution digestion coupled with lc-ms/ms. a total of 27-52 proteins, categor ... | 2018 | 29929037 |
| anti-deinagkistrodon acutus venom properties of ethanolic root extract from cynanchum paniculatum (bunge) kitag and its gc-ms analysis. | cynanchum paniculatum (bunge) kitag known as a popular chinese herbal medicine has been used for a long time to treat a wide variety of diseases including snakebites. however, there is scarce information on the antiophidian potential of this plant. | 2018 | 30033377 |
| inactivation of venom pla₂ alleviates myonecrosis and facilitates muscle regeneration in envenomed mice: a time course observation. | snake venom is a complex cocktail of toxins which induces a series of clinical and pathophysiological manifestations in victims, including severe local tissue damage and systemic alterations. deinagkistrodon acutus (d. acutus) ranks among the "big four" life-threatening venomous species in china, whose venom possesses strong myotoxicity and hematotoxicity that often lead to permanent disability or muscle atrophy. varespladib, an inhibitor of mammalian phospholipase a₂ (pla₂), has been recently r ... | 2018 | 30065214 |
| purification and characterization of a novel antiplatelet peptide from deinagkistrodon acutus venom. | animal venoms are considered as one of the most important sources for drug development. deinagkistrodon acutus is famous for its toxicity to the human hematological system and envenomed patients develop a coagulation disorder with the symptoms of hemorrhage and microthrombi formation. the purpose of this study was to separate antiplatelet peptides from d. acutus venom using a combination of an ultrafiltration technique and reversed-phase high performance liquid chromatography (hplc), which was g ... | 2018 | 30115892 |
| differential heme-mediated modulation of deinagkistrodon, dispholidus, protobothrops and pseudonaja hemotoxic venom activity in human plasma. | envenomation by vipers with hemotoxic enzymes continues to be a worldwide source of morbidity and mortality. the present work examined the effects of exposure of venom enzymes to carbon monoxide and o-phenylhydroxylamine, agents that modulate the biometal heme, by forming carboxyheme and metheme, respectively. four venoms obtained from medically important, diverse snake venom found in africa, asia and australia were analyzed. the species that had venom tested in human plasma with thrombelastogra ... | 2018 | 30132273 |
| a fatal diarrhoea outbreak in farm-raised deinagkistrodon acutus in china is newly linked to potentially zoonotic aeromonas hydrophila. | deinagkistrodon acutus is a venomous pit viper commonly used in traditional chinese medicine; farming these snakes has become a major industry. in 2017, an outbreak of fatal diarrhoea among farm-raised d. acutus in hunan province caused the deaths of 5,600 snakes within 3 weeks. we isolated a brand-new sequence type of aeromonas hydrophila (st516) from lesions and confirmed that this bacterium was the causal agent of the outbreak. snakes infected with the bacterium in the laboratory showed simil ... | 2019 | 30222905 |
| snake venom proteome and immuno-profiling of the hundred-pace viper, deinagkistrodon acutus, in taiwan. | deinagkistrodon acutus, also known as the hundred-pace viper or chinese moccasin, is a clinically significant venomous snake in taiwan. to address the lack of knowledge on the venom proteome of d. acutus, the venom composition was studied by a bottom-up proteomic approach combining reverse phase high-performance liquid chromatography, sds-page, and lc-ms/ms analysis. the immunoreactivity and cross-reactivity of taiwanese freeze-dried d. acutus antivenom (da-av) and hemorrhagic antivenom (fh-av) ... | 2019 | 30268686 |
| haemostatic bioactivity of novel schizonepetae spica carbonisata-derived carbon dots via platelet counts elevation. | schizonepetae spica carbonisata (ssc) has pronounced haemostatic effects for hundreds of years and has been acknowledged in the 2015 pharmacopoeia of the people's republic of china (pprc) as a haemostatic charcoal drug. however, after years of efforts, the underlying mechanism and the material basis is still less defined. in this research, we developed a novel cds derived from ssc (ssc-cds) with an average diameter of 1.29-6.87 nm and a quantum yield of 6.31%. ssc was prepared using a modified p ... | 2018 | 30431371 |
| agkistrodon ameliorates pain response and prevents cartilage degradation in monosodium iodoacetate-induced osteoarthritic rats by inhibiting chondrocyte hypertrophy and apoptosis. | osteoarthritis (oa), characterized by joint pain and cartilage degradation, is the most common form of joint disease worldwide but with no satisfactory therapy available. the ethanol extract of agkistrodon acutus (eaa) has been widely used as a traditional chinese medicine (tcm) for the treatment of arthralgia and inflammatory diseases, but there is no report regarding its efficacy on oa to date. here, we determined the effects of eaa on the pain behavior and cartilage degradation in vivo and cl ... | 2019 | 30529425 |
| clinical and laboratory features distinguishing between deinagkistrodon acutus and daboia siamensis envenomation. | there are 6 species of venomous snakes in taiwan. two of them, deinagkistrodon acutus (d. acutus) and daboia siamensis (d. siamensis), can cause significant coagulopathy. however, a significant proportion of patients with snakebites cannot identify the correct snake species after envenomation, which hampers the application of antivenom. hence, the differential diagnosis between the two snakebites by clinical presentations is important. this study aims to compare their clinical and laboratory fea ... | 2018 | 30607144 |
| hemostatic and hepatoprotective bioactivity of junci medulla carbonisata-derived carbon dots. | to explore the hemostatic and hepatoprotective bioactivity of junci medulla carbonisata-derived carbon dots (jmc-cds). | 2019 | 30698498 |
| basal but divergent: clinical implications of differential coagulotoxicity in a clade of asian vipers. | envenomations by asian pitvipers can induce multiple clinical complications resulting from coagulopathic and neuropathic effects. while intense research has been undertaken for some species, functional coagulopathic effects have been neglected. as these species' venoms affect the blood coagulation cascade we investigated their effects upon the human clotting cascade using venoms of species from the azemiops, calloselasma, deinagkistrodon and hypnale genera. calloselasma rhodostoma, deinagkistrod ... | 2019 | 30930232 |
| comparative analysis and characterization of the gut microbiota of four farmed snakes from southern china. | the gut microbiota plays an important role in host immunity and metabolic homeostasis. although analyses of gut microbiotas have been used to assess host health and foster disease prevention and treatment, no comparative comprehensive study, assessing gut microbiotas among several species of farmed snake, is yet available. in this study, we characterized and compared the gut microbiotas of four species of farmed snakes (naja atra, ptyas mucosa, elaphe carinata, and deinagkistrodon acutus) using ... | 2019 | 30956901 |
| functional recombinant single-chain variable fragment antibody against agkistrodon acutus venom. | agkistrodon acutus bites are conventionally treated with animal-derived antivenom, the use of which is limited due to allergic reactions and serum sickness. thus in the present study, the genes of humanized antibodies produced in response to a. acutus venom were extracted from lymphocytes from patients bitten by a. acutus. a single-chain variable fragment (scfv) library against venom was constructed using a t7 phage display system. scfv genes that exhibited high affinity to venom were selected b ... | 2019 | 30988762 |
| ameliorative effects of snake (deinagkistrodon acutus) oil and its main fatty acids against uvb-induced skin photodamage in mice. | the effects of topically administered snake (deinagkistrodon acutus) oil and its main fatty acid components on skin photodamage were explored. epidermal thickness, mice body weight, antioxidant enzymes (superoxide dismutase, glutathione peroxidase, catalase), inflammatory cytokines (tumor necrosis factor alpha and interleukin-6), skin histology, collagen content, and metalloproteinase-1 indicators were analyzed. the results show that topical application of both snake oil and its main fatty acids ... | 2019 | 31247385 |
| isolation of a novel metalloproteinase from agkistrodon venom and its antithrombotic activity analysis. | snake venom contains large amounts of active proteins and peptides. in this study, a novel snake protein, metalloproteinase sp, was successfully isolated from the venom of agkistrodonacutus by multi-gel chromatography. the isolated protein exhibits anti-platelet aggregation activity. animal experiments showed that it exhibited defibration, anticoagulation, and antithrombotic effects and contributes to improved blood rheology and antiplatelet aggregation. in vivo experiments demonstrated that it ... | 2019 | 31438579 |
| protective effects of carbon dots derived from phellodendri chinensis cortex carbonisata against deinagkistrodon acutus venom-induced acute kidney injury. | as an emerging nanomaterial, carbon dots (cds) have been the focus of tremendous attention for biomedical applications. however, little information is available on their bioactivity of inhibiting acute kidney injury (aki) induced by snake venom. | 2019 | 31845094 |
| [transcriptome analysis of venom gland and identification of functional genes for snake venom protein in agkistrodon acutus]. | agkistrodon acutus is a traditional chinese herb medicine which has immunological regulation,anti-tumor,anti-inflammatory and analgesic effects,which is mainly used for the treatment of rheumatoid arthritis,ankylosing spondylitis,sjogren's syndrome and tumors. in order to excavate more important functional genes from a. acutus,the transcriptome of the venom gland was sequenced by the illumina hi seq 4000,and 32 862 unigenes were assembled. among them,26 589 unigenes were mapped to least one publ ... | 2019 | 31872588 |
| aavc-i promotes apoptosis of human oral squamous cell carcinoma through the mitochondrial pathway. | this study aimed to explore the role and possible mechanism of component i from agkistrodon acutus venom (aavc-i) in promoting the apoptosis of oral squamous cell carcinoma (oscc). hn4 cells (a human oscc cell line) were randomly divided into four experimental groups: low aavc-i (2.5 μg/ml) group, medium aavc-i (5 μg/ml) group and high aavc-i (10 μg/ml) group, as well as control group (aavc-i, 0 μg/ml). avvc-i was dissolved in rpmi-1640 medium and added to the culture wells at different concentr ... | 2019 | 31933792 |
| antivenom neutralization of coagulopathic snake venom toxins assessed by bioactivity profiling using nanofractionation analytics. | venomous snakebite is one of the world's most lethal neglected tropical diseases. animal-derived antivenoms are the only standardized specific therapies currently available for treating snakebite envenoming, but due to venom variation, often this treatment is not effective in counteracting all clinical symptoms caused by the multitude of injected toxins. in this study, the coagulopathic toxicities of venoms from the medically relevant snake species bothropsasper, calloselasmarhodostoma, deinagki ... | 2020 | 31963329 |
| radial artery aneurysm formation and spontaneous rupture after snake bite to the right forearm. | to report the outcome of a patient who developed aneurysm after a deinagkistrodon acutus snakebite. | 2020 | 32360670 |
| multiplex ligation-dependent probe amplification for simultaneous identification of bungarus multicinctus and its common adulterants in a single assay. | bungarus multicinctus, an important traditional chinese medicine, possesses remarkable medicinal activities, while lots of adulterants from other species were misused as b. multicinctus for its large demand and resource starvation. in order to accurately identify b. multicinctus and its common adulterants such as sinonatrix annularis, xenochrophis flavipunctatus, deinagkistrodon acutus, and naja atra, a simultaneous identification method was designed with multiplex ligation-dependent probe ampli ... | 2020 | 32372964 |
| evolutionary interpretations of nicotinic acetylcholine receptor targeting venom effects by a clade of asian viperidae snakes. | ecological variability among closely related species provides an opportunity for evolutionary comparative studies. therefore, to investigate the origin and evolution of neurotoxicity in asian viperid snakes, we tested the venoms of azemiops feae, calloselasma rhodostoma, deinagkistrodon acutus, tropidolaeums subannulatus, and t. wagleri for their relative specificity and potency upon the amphibian, lizard, bird, rodent, and human α-1 (neuromuscular) nicotinic acetylcholine receptors. we utilised ... | 2020 | 32394055 |
| purification and characterization of protein c activator from agkistrodon acutus venom. | protein c (pc) plays an important role in the balance of coagulation and anticoagulation. thus, the detection of pc activity is diagnostically significant for patients with cardiovascular diseases. presently, the key methods to detect pc activity are the chromogenic assay and activated partial thromboplastin time (aptt) test. protac used in the chromogenic assay is isolated from agkistrodon contortrix venom as protein c activator (pca). however, the use of the chromogenic assay is limited becaus ... | 2020 | 32478599 |
| varespladib inhibits the phospholipase a2 and coagulopathic activities of venom components from hemotoxic snakes. | phospholipase a2 (pla2) enzymes are important toxins found in many snake venoms, and they can exhibit a variety of toxic activities including causing hemolysis and/or anticoagulation. in this study, the inhibiting effects of the small molecule pla2 inhibitor varespladib on snake venom pla2s was investigated by nanofractionation analytics, which combined chromatography, mass spectrometry (ms), and bioassays. the venoms of the medically important snake species bothrops asper, calloselasma rhodosto ... | 2020 | 32560391 |
| targeted identification of c-type lectins in snake venom by 2de and western blot. | c-type lectins (ctl) and ctl-like proteins (snaclecs) are important toxins found in snake venom which can disrupt hemostasis by binding platelet membrane glycoproteins. traditional identification of these toxins usually relies on an "activity-directed fractionation" approach which is very arduous. here, we report a new method for rapid screening of these proteins in snake venom. | 2020 | 32598989 |
| an examination of the neutralization of in vitro toxicity of chinese cobra (naja atra) venom by different antivenoms. | the chinese cobra (naja atra) is an elapid snake of major medical importance in southern china. we describe the in vitro neurotoxic, myotoxic, and cytotoxic effects of n. atra venom, as well as examining the efficacy of three chinese monovalent antivenoms (n. atra antivenom, gloydius brevicaudus antivenom and deinagkistrodon acutus antivenom) and an australian polyvalent snake antivenom. in the chick biventer cervicis nerve-muscle preparation, n. atra venom (1-10 µg/ml) abolished indirect twitch ... | 2020 | 32992934 |
| oxidative stress and antioxidant defense in detoxification systems of snake venom-induced toxicity. | snakebites remain a major life-threatening event worldwide. it is still difficult to make a positive identification of snake species by clinicians in both western medicine and chinese medicine. the main reason for this is a shortage of diagnostic biomarkers and lack of knowledge about pathways of venom-induced toxicity. in traditional chinese medicine, snakebites are considered to be treated with wind, fire, and wind-fire toxin, but additional studies are required. | 2020 | 33117427 |
| neutralising effects of small molecule toxin inhibitors on nanofractionated coagulopathic crotalinae snake venoms. | repurposing small molecule drugs and drug candidates is considered as a promising approach to revolutionise the treatment of snakebite envenoming. in this study, we investigated the inhibiting effects of the small molecules varespladib (nonspecific phospholipase a2 inhibitor), marimastat (broad spectrum matrix metalloprotease inhibitor) and dimercaprol (metal ion chelator) against coagulopathic toxins found in crotalinae (pit vipers) snake venoms. venoms from bothrops asper, bothrops jararaca, c ... | 2020 | 33163338 |
| structure of the platelet glycoprotein ib receptor in complex with a novel antithrombotic agent agkisacucetin. | agkisacucetin is a snake c-type lectin-like protein (snaclec) isolated from the venom of deinagkistrodon acutus (formerly agkistrodon acutus), which is a novel anti-thrombotic drug candidate currently in phase 2 clinical trials. agkisacucetin specifically recognizes the platelet receptor glycoprotein ib α chain (gpibα) to block gpib and von willebrand factor (vwf) interaction. in this study, we solved the crystal structure of gpibα n-terminal domain (residue 1-305) in complex with agkisacucetin ... | 2020 | 33181828 |