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[microbiological composition of dental plaque using sprague dawley rats as an experimental model].in this study, the microbiological composition of the dental plaque in 12 male sprague-dawley rats was determined. analysis using the light microscope showed the presence of nine colonies which suggested the presence of cocci, (6) diplococci (1) and rods. (2) five of the bacteria were gram positive and three were gram negative. the morphological characteristic suggested the presence of actinomyces in the case of gram positive rods; fusobacterium in the case of gram negative rods; neisseria and v ...19902131736
[morpho-functional characteristics of bacterial species of major importance in endodontic infections].most of the bacteria involved in the endodontic infection can be considered as "highly specific", for they appear to be almost irrelevant in the large field of the other human disease from infection. namely the most important anaerobia responsible for endodontic infection are: bacteroides, fusobacterium, peptostreptococcus, and veillonella. the group of bacteroides is composed by several species with different metabolic and genetic characteristics. they are gram-, non-motile, non-spore-forming r ...19902133812
[immunobiological activities of veillonella parvula isolated from infected root canals].the primary etiological agent of all periapical lesions has long been considered to be bacterial. furthermore, bacteria from infected root canals are potential antigens capable of initiating immunological reactions in periapical tissues. the purpose of this study, therefore, was clarify the immunological potentials of veillonella parvula (v. parvula), which was frequently isolated from root canals with periapical lesion. immunobiological activities of v. parvula sonic extracts were investigated ...19902134271
characterization of the tet m determinants in urogenital and respiratory bacteria.tetracycline-resistant fusobacterium nucleatum, haemophilus ducreyi, mycoplasma hominis, peptostreptococcus spp., ureaplasma urealyticum, and veillonella parvula had dna sequences which showed homology throughout the length of the tet m transposon, tn916. in contrast, gardnerella vaginalis, commensal neisseria spp., and the 25.2-megadalton plasmid family lacked the complete transposon.19902159259
effects of fluoride on the microbial ecology of dental plaque.in order to survive in a community such as dental plaque, bacteria must be able to resist changes in the environment. these changes may be the result of differences in the host or the activities of the bacteria within the community. bacteria which cannot resist these environmental pressures may cease metabolism but survive, or be eliminated from the community. fluoride is only one of numerous environmental pressures which impact on oral bacterial communities, yet its beneficial effect on caries ...19902179326
the microbiology of periapical granulomas.of the 16 periapical granulomas studied, 14 (88%) yielded a positive growth when homogenized and cultured. the concentration of colony-forming units per milliliter of the suspension ranged from 10(1.3) to 10(4.0) (mean 10(2.2)). a total of 47 isolates comprising 26 (55%) facultative anaerobes and 21 (45%) strict anaerobes were obtained. the organisms most commonly cultured were veillonella species (15%), streptococcus milleri (11%), streptococcus sanguis (11%), actinomyces naeslundii (11%), prop ...19902183126
microbiology of root surface caries in humans.studies on the microbiology of root surface caries between 1970 and 1975 placed emphasis on gram-positive pleomorphic filamentous rods, particularly actinomyces viscosus and actinomyces naeslundii. both of these species had been shown to produce root surface caries in experimental animals. since this time, studies have placed more emphasis on streptococcus mutans, and s. mutans and lactobacillus are significant in prediction of root surface caries risk in patients. subsequent studies confirmed a ...19902186069
a multicentre study to evaluate the effect of sulbactam/ampicillin combination on anaerobic micro-organisms.ampicillin combined with the beta-lactamase inhibitor sulbactam was compared with ampicillin alone, cefoxitin and metronidazole against 569 clinical strains of anaerobic organisms. the strains included 289 species of bacteroides, 160 strains of clostridium and 120 strains of various species of streptococcus/peptostreptococus, fusobacterium, veillonella, eubacterium, bifidobacterium, actinomyces and propionibacterium. sulbactam/ampicillin was as effective as cefoxitin and metronidazole against al ...19902193834
recolonization of the subgingival microflora after scaling and root planing in human periodontitis.the purpose of this study was to evaluate the recolonization patterns of the subgingival microflora of adult periodontitis patients after a single session of scaling and root planing. in each of eight patients, three clinically diseased sites were investigated microbiologically by darkfield microscopy and cultural analysis. after initial clinical and microbiological parameters were determined, each subject received a single session of scaling and root planing but no oral hygiene instructions. cl ...19902213468
an intra-oral appliance study of the plaque microflora associated with early enamel demineralization.an intra-oral appliance model was used to investigate the composition of the plaque microflora associated with early enamel demineralization. enamel sections, with exposed windows, were mounted on lower removable appliances, and the devices were worn by volunteers for three-week periods under three experimental conditions. these were: (1) "normal" plaque conditions, (2) extra-oral sucrose applications nine times daily, and (3) inoculation of each volunteer's own mutans streptococci onto the test ...19902229608
changes in subgingival microbiota during puberty. a 4-year longitudinal study.it was the purpose of the present investigation to monitor the composition of the subgingival microbiota at selected sites in individuals passing through puberty and to correlate observed changes with the development of pubertal maturation. between the ages of 11 and 14 years, pubertal and skeletal maturation was monitored annually in 22 boys and 20 girls. during this time, subgingival microbial samples were taken every 4th to 5th month (10 times in 4 years) mesially of the upper first molars. h ...19902262580
chemical analyses, local shwartzman reactivity, and body weight-decreasing activity of aqueous-phenol extracts of mycoplasma salivarium cells. biological activities of mycoplasma salivarium.aqueous-phenol extracts of mycoplasma salivarium atcc 23064 cells (apm) showed demonstrable differences from lipopolysaccharides (lpss) of veillonella rodentium atcc 17743. these were as follows: smaller amounts of amino sugars and an absence of 2-keto-3-deoxyoctonate; local shwartzman reactivity and body weight-decreasing activity, even though the activities were rather weak compared with those of lpss. therefore, phenol-water extractive components of mycoplasma salivarium might be of pathogeni ...19902264725
obligately anaerobic bacterial species isolated from foot-rot lesions in goats.lesions showing clinical signs of foot-rot from 120 goats were cultured on six selective media during october 1987 to november 1988. a total of 582 strictly anaerobic microorganisms belonging to 50 different species was isolated and identified. the anaerobes most frequently isolated belonged to the following genera: bacteroides (80%), peptostreptococcus (63.6%), megasphaera (40%), fusobacterium (29.2%), clostridium (22.5%), propionibacterium (12.5%), eubacterium (11.7%) and leptotrichia (10.8%). ...19902271912
endocarditis caused by veillonella dispar. 19902273279
bacteremia due to anaerobic bacteria in newborns.awareness of the role of anaerobic bacteria in neonatal bacteremia has increased in recent years. the incidence of recovery of anaerobes in neonatal bacteremia varies, according to different studies, between 1.8% and 12.5%. of the 178 cases reported in the literature, 73 were due to bacteroides species (69 were the bacteroides fragilis group), 57 clostridium species (mostly clostridium perfringens), 35 peptostreptococci, 5 propionibacterium acnes, 3 veillonella species, 3 fusobacterium species, ...19902277280
microbial patterns in pooled subgingival plaque samples from young adults with advanced marginal periodontitis.different bacterial profiles of subgingival plaque have been described for different clinical categories of marginal periodontitis. in the present investigation the subgingival microflora was studied in young adults with advanced marginal periodontitis. from 12 patients pooled subgingival plaque samples from 5 advanced stages of diseased sites were examined by direct differential phase-contrast microscopy and by cultivation on enriched and selective media. the proportions of the following genera ...19902293348
salivary levels of mutans streptococci, lactobacillus, candida, and veillonella species in a group of scottish adolescents.salivary levels of mutans streptococci, lactobacillus, candida, and veillonella species were investigated on three occasions at annual intervals in a group of 372 scottish adolescents. counts of the micro-organisms studied were logarithmically distributed, with candida spp. being isolated from approximately half the subjects. counts of lactobacilli, mutans streptococci, and candida were significantly intercorrelated, while veillonella were associated consistently with mutans streptococci alone. ...19902297975
colonization resistance of defined bacterial plaques to streptococcus mutans implantation on teeth in a model mouth.we investigated the ability of streptococcus mutans c67-1 to colonize simple bacterial plaques and the effects of age and stability of the pre-formed plaque on colonization resistance. mixed-plaques of actinomyces viscosus wvu627, 'streptococcus mitior' lpa-1, and veillonella dispar omz193 were grown on tooth segments, mounted back to back for simulation of approximal sites in a model mouth for 66 h. s. mutans c67-1 was either included in the original inoculum or super-inoculated onto the develo ...19902307751
growth stimulation of treponema denticola by periodontal microorganisms.previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of treponema denticola. t. denticola depends on bacterial interactions for its growth in serum. aim of the present study was to identify specific microorganisms involved in the growth stimulation of t. denticola. to this end, strains isolated from previous plaque enrichment cultures were tested for growth stimulation in co-cultures with t. denticola. in addition, growth of t. den ...19902321929
susceptibility and resistance of plaque bacteria to minocycline.the in vitro susceptibility of 55 strains of subgingival plaque bacteria to minocycline was determined. a concentration of 1 microgram/ml minocycline was found to inhibit 85% of the strains tested and the mic ranged from 0.03 to 32 micrograms/ml. for 71% of the strains tested the mbc was at least 4 times greater than the corresponding mic, suggesting a bacteriostatic activity for minocycline. a concentration of 20 mg/ml of magnesium ions was capable of neutralizing 8 micrograms/ml of minocycline ...19902324922
surface-associated properties of streptococcus milleri group strains and their potential relation to pathogenesis.thirty strains from the streptococcus milleri (anginosus) group (smg) obtained from various sources were tested for a range of characters that could be associated with pathogenicity and the results were compared with those for type strains of s. sanguis, s. mutans and s. pyogenes. the smg strains were heterogeneous in all tests. most (18) belonged to one of the lancefield groups with group f predominating. adherence of strains isolated from abscesses to buccal epithelial cells was greater than t ...19902325115
relationship of bacteriologic characteristics to semen indices in men attending an infertility clinic.bacteria can be isolated from most seminal fluid samples, but the significance of these microorganisms is uncertain because most men lack symptoms associated with bacterial infection of the reproductive tract. we obtained semen samples from 37 men attending a special infertility clinic and assessed the relationship between seminal fluid microorganisms and seminal fluid analysis including sperm motility, morphology, and concentration; the numbers of polymorphonuclear leukocytes and other white bl ...19902325963
genetic basis of tetracycline resistance in urogenital bacteria.the distributions of the nucleotide sequences related to the tetracycline resistance determinants tet k, tet l, tet m, and tet o were studied by dot blot hybridization with randomly chosen clinical urogenital tract isolates of viridans group streptococci, streptococcus agalactiae, enterococcus faecalis, gardnerella vaginalis, lactobacillus spp., fusobacterium nucleatum, peptostreptococcus spp., and veillonella parvula. among the peptostreptococcus spp., 79% of the isolates hybridized with one (6 ...19902327774
in vitro activity of meropenem (sm-7338), imipenem, and five other antibiotics against anaerobic clinical isolates.the in vitro susceptibility of 513 recent anaerobic clinical isolates was evaluated against meropenem (sm-7338), a new carbapenem, and six other antibiotics. virtually all gram-positive and gram-negative anaerobic bacteria tested were susceptible to meropenem (defined as mics less than or equal to 8 micrograms/ml) with 99.8% of the isolates inhibited by less than or equal to 4 micrograms/ml. the activity of meropenem was comparable to imipenem for most clinical isolates. minor differences were o ...19902331851
microflora associated with experimental root surface caries in humans.this study describes the microflora from actively progressing root surface caries lesions, in which mineral loss had been determined by quantitative microradiography. the caries lesions were produced experimentally in root surface specimens from human molars inserted in lower partial dentures carried for 3 months by six elderly individuals. a total of 780 bacterial isolates were identified from 13 plaque samples, collected with a punch technique, and six dentin samples. the composition of the mi ...19902341169
caries prevalence and microbiological and salivary caries activity tests in scottish adolescents.salivary and microbiological caries activity tests were investigated on three occasions in a group of 372 scottish adolescents. counts of lactobacilli, mutans streptococci, and candida were consistently and significantly associated with caries prevalence, as either ds or dmfs score, and buffering capacity was consistently inversely related to dmfs score. however, veillonella counts and salivary flow rate were not correlated with caries prevalence. significant improvements in the associations wer ...19902350947
[comparative antibacterial activity of cefpodoxime, cefuroxime and cefaclor against strict anaerobic bacteria].the in vitro activity of a new cephalosporin (cpx) was assessed against 260 strains of anaerobic bacteria. a comparison was done with two other oral cephalosporins cefaclor (cfa) and cefuroxime (cxm). mics were determined by an agar-dilution method on wilkins chalgren agar. the three antibiotics had little activity against bacteroïdes fragilis group. cefpodoxime was more active than the two other cephalosporins against fusobacterium and bacteroïdes spp. at 4 mg/l concentration the percentages of ...19902367150
an investigation into the mechanism of protection by local passive immunization with monoclonal antibodies against streptococcus mutans.local oral passive immunization with streptococcus mutans-specific monoclonal antibody (mab) (guy's 13) prevented recolonization by indigenous s. mutans in human volunteers who had first been treated with a conventional antibacterial agent (chlorhexidine). the f(ab')2 fragment of the mab was as protective as the intact immunoglobulin g, but the fab fragment of the molecule failed to prevent recolonization of s. mutans. in subjects receiving the mab fab fragment, s. mutans levels in dental plaque ...19902401569
ruthenium red staining reveals surface fibrils and a layer external to the cell wall in streptococcus salivarius hb and adhesion deficient mutants.ruthenium red staining revealed both the long and short classes of cell surface fibril in thin sections of streptococcus salivarius hb, indicating that the fibrils contained polyanionic polymers, probably polysaccharides. also visible was a 16.2 +/- 2.2 nm thick ruthenium red staining layer (rrl) outside the 16.7 +/- 2.2 nm thick cell wall. the fibrils could not be seen after conventional glutaraldehyde and osmium fixation. the rrl was protease resistant and was not involved in septum formation. ...19882479709
[isolation of anaerobes during a 30-month observation at a hospital microbiology laboratory].the authors evaluate retrospectively the results obtained from the research of anaerobial bacteria on 1313 samples received at the microbiology laboratory of the "ospedale civile di ivrea" over a period of 31 months (6/1/86-12/31/88). from this evaluation, high percentages of detection of anaerobic bacteria are emerging in the following infections: appendiculare abscesses (60%), intestinal operations (71%), wounds (57%), tubovarian abscesses (100%), as well as thoracic empyema (50%). also releva ...19892490397
veillonellae: personal observation of a fatal bacteremia. 19892491305
bacterial meningitis caused by veillonella parvula.a three-year-old girl injured her right eyelid with a toothbrush. the wound was sutured. swelling of the eyelid, high fever and vomiting developed in spite of oral antibiotics for seven days. the findings of the cerebrospinal fluid (csf) were white blood cells (wbc) 26,368/mm3 (90% polymorphs), protein 127 mg/dl, and sugar 0 mg/dl. although gram negative organisms were seen on the smear, aerobic culture was sterile. later culture of csf on admission grew anaerobic bacteria: veillonella parvula. ...19892515742
purification and characterization of bacteriophage receptor on veillonella rodentium cells. phage-receptor on veillonella.veillonellophage n2 adsorbed to polysaccharides (pss) on veillonella rodentium atcc 17743 cell wall, and the bacteriophage receptor contained only glucosamine. d(+)-glucosamine hydrochloride (sigma) also adsorbed the veillonellophage n2. these results therefore indicate that the receptor to the veillonellophage n2 is cell wall pss. the pss of the host cells as receptor have been characterized. glucosamine accounted for approximately 100% of the weight of the pss. the pss which were partially res ...19892589854
the microflora associated with the development of initial enamel decalcification below orthodontic bands in vivo in children living in a fluoridated-water area.thirty-four caries-free teeth destined for orthodontic extraction were banded to provide a protected area for the accumulation of plaque. the teeth were extracted at one, two, four, eight, and 14 days after being banded, and samples of the flora below the band were analyzed for the presence of streptococcus sanguis, streptococcus 'mitior', 'mutans streptococci', actinomyces viscosus, actinomyces naeslundii, actinomyces odontolyticus, lactobacillus species, and veillonella. after plaque sampling, ...19892600252
metabolism of gentiopicroside (gentiopicrin) by human intestinal bacteria.as a part of our studies on the metabolism of crude drug components by intestinal bacteria, gentiopicroside (a secoiridoid glucoside isolated from gentiana lutea), was anaerobically incubated with various defined strains of human intestinal bacteria. many species had ability to transform it to a series of metabolites. among them, veillonella parvula ss parvula produced five metabolites, which were identified as erythrocentaurin, gentiopicral, 5-hydroxymethylisochroman-1-one,5-hydroxymethylisochr ...19892605687
an in vitro method to study the adherence of oral bacteria to hela cells.exfoliated buccal epithelial cells have been widely used in microbial adherence studies, but present a number of problems due to their variable nature and to contamination with indigenous bacteria. an adherence assay was developed using hela cell monolayers which were washed with buffer, or treated with saliva or serum to mimic buccal or crevicular epithelial cells, respectively. a total of eighteen strains of oral bacteria tested showed a low affinity for untreated hela cells, but most strains ...19892607993
the predominant cultivable microbiota of crevicular epithelial cells.the purpose of the present investigation was to compare the composition of the predominant cultivable microbiota associated with gingival crevicular epithelial cells with that of the unattached microbiota recovered from the same site. samples were taken from 2 diseased sites from 8 periodontal patients, by scraping the epithelial lining of the pocket with a curette. the epithelial cells were separated from the unattached subgingival bacteria by centrifugation in a reduced 50% percoll density gra ...19892628861
a microbiological study of early caries of approximal surfaces in schoolchildren.a cross-sectional epidemiological study has been undertaken to relate the bacterial composition of approximal dental plaque with the earliest stages of caries development in schoolchildren. small samples of plaque were removed from multiple sites around the contact areas of 42 premolars extracted for orthodontic reasons from 29 schoolchildren (mean age = 13.5 yr). caries diagnosis was based on polarized light microscopy and contact microradiography of thin sections cut through the sample sites. ...19892632599
expression of the surface properties of the fibrillar streptococcus salivarius hb and its adhesion deficient mutants grown in continuous culture under glucose limitation.streptococcus salivarius hb and four adhesion deficient mutants, hb-7, hb-v5, hb-v51 and hb-b, were grown in continuous culture in a defined medium under glucose limitation over a range of growth rates from 0.1 to 1.1 h-1. the ability to coaggregate with veillonella parvula v1 cells and the ability to adhere to buccal epithelial cells did not alter with increasing growth rate. cell surface hydrophobicity decreased markedly with increasing growth rate for the non-fibrillar non-adhesive mutant hb- ...19892632665
[comparative study of the antimicrobial effect of various cavity liners used in conservative dentistry].we have compared the microbiological activity of the following cavity liners: life, dycal ii, calcipulpe, pure calcium hydroxide and cavitec; against five different bacterial strains: veillonella parvula, bacteroides fragilis, peptococcus s.p., staphylococcus aureus, and streptococcus beta hemolytic: the results demonstrate the higher antimicrobial activity of the manufactured cavity liners with calcium hydroxide base in comparison with the pure calcium hydroxide.19892638022
detection of immunodominant antigens of periodontopathic bacteria in human periodontal disease.sonicated whole cell extracts and outer membrane proteins (omp) from bacteroides gingivalis and veillonella parvula were analysed by the immunoblot technique using sera from 103 patients with various forms of periodontal disease and from 31 control subjects. b. gingivalis sonicate contained 12 major bands (75-14 kda) of which the 46, 27 and 14 kda antigens reacted more frequently with sera from adult and young adult patients with severe periodontitis compared with sera from controls and mild per ...19892639300
cytotoxicity against various cell lines of lipopolysaccharides purified from bacteroides, fusobacterium, and veillonella isolated from infected root canals.the cytotoxicity against two mesenchymal cell lines, l-929 and wi-38, and two epithelial cell lines, kb and hela s-3, of lipopolysaccharide (lps) purified from strains of bacteroides gingivalis, fusobacterium nucleatum, and veillonella parvula isolated from infected root canals was investigated. the inhibition of cell growth by these lps's was considerable for mesenchymal cell lines, but mild for epithelial cell lines. the cytotoxic effect of f. nucleatum lps was the greatest and that of b. ging ...19892639950
subgingival microflora, dental and periodontal conditions in patients with hereditary fructose intolerance.hereditary fructose intolerance (hfi) is a rare autosomally recessive disease which leads to severe hypoglycemia. the fructose-free diet of these patients apparently influences dental health. half of the patients are free of caries, but there are no reports on their periodontal condition nor on the composition of their subgingival microflora. therefore 18 patient with hfi were examined for the following parameters: radiographic bone loss, caries rate, gingival bleeding, occurrence of calculus, p ...19892704977
a cervicofacial infection due to veillonella parvula in a patient with myxoedema. 19892713184
'a cervicofacial infection due to veillonella parvula in a patient with myxoedema'. 19892751904
bacterial flora in chronic tonsillitis.a study of aerobic and anaerobic bacterial flora in chronic tonsillitis revealed a total of 59 isolates. aerobic flora from the surface swab was found in 80%, from the core of the tonsil in 92% and from both the surface and the core in 68% of the cases. there were 30 aerobic isolates from the surface and 29 isolates from the core. mixed flora were present from the surface and the core in 10 and 7 cases, respectively. pathogens were seen in 36% of the cases from the surface and in 40% from the co ...19892759695
colony counts and characterization of bacteria adherent to the rumen wall and desquamated epithelial cells in conventional young lambs.characterization and enumeration of the adherent epimural community of the rumen wall of young, conventionally reared lambs were carried out from 2 to 21 days after birth. three hundred strains were isolated by anaerobic procedures from three sites: dorsal, ventral, and caudal sacs, and from the sloughed epithelial cells. the population of epimural bacteria was very dense from the first days of the lamb's life. this population increased slightly with age. during the first week the counts were si ...19892766120
coaggregation of fusobacterium nucleatum, selenomonas flueggei, selenomonas infelix, selenomonas noxia, and selenomonas sputigena with strains from 11 genera of oral bacteria.twenty-eight strains of fusobacterium nucleatum and 41 selenomonas strains, including s. sputigena (24 strains), s. flueggei (10 strains), s. infelix (5 strains), and s. noxia (2 strains), were tested for their ability to coaggregate with each other and with 49 other strains of oral bacteria representing actinobacillus, actinomyces, bacteroides, capnocytophaga, gemella, peptostreptococcus, porphyromonas, propionibacterium, rothia, streptococcus, and veillonella species. selenomonads coaggregated ...19892777378
effect of periodontal status of rotary electric toothbrushes vs. manual toothbrushes during periodontal maintenance. ii. microbiological results.we compared the use of a rotary electric toothbrush with conventional toothbrushing for their effectiveness in modulating the subgingival microbiota. forty subjects who had received periodontal treatment for moderate to advanced periodontitis and were on 3-month periodontal maintenance were divided into two equal groups matched for age and sex. one group used only the rotary tooth cleaner and the other group used conventional toothbrushing, dental floss, and toothpicks for 12 months. subgingival ...19892778609
bilateral abscessed orchiepididymitis associated with sepsis caused by veillonella parvula and clostridium perfringens: case report and review of the literature.veillonella species is a gram-negative coccus which is part of the anaerobic normal flora in the oral cavity, small intestine, upper respiratory tract, vagina, and urinary tract. the role that this organism plays in infection is not well known, and it is generally associated with other bacteria. we present a case of bilateral abscessed orchiepididymitis associated with septicemia due to veillonella parvula and, later, to clostridium perfringens, with the development of severe renal insufficiency ...19872887584
a comparison of the adhesion, coaggregation and cell-surface hydrophobicity properties of fibrillar and fimbriate strains of streptococcus salivarius.fibrillar and fimbriate strains of streptococcus salivarius were compared for their ability to adhere to buccal epithelial cells and saliva-coated hydroxyapatite beads, and for their ability to coaggregate with veillonella strains. the fibrillar lancefield group k strains adhered statistically significantly better to both buccal epithelial cells and saliva-coated hydroxyapatite beads than the fimbriate strains, which lacked the lancefield group k antigen. after 1 h the fibrillar strains coaggreg ...19872895798
the bacteriology of bronchiectasis in hong kong investigated by protected catheter brush and bronchoalveolar lavage.bacteria often colonize the lower respiratory tract of patients with bronchiectasis. although the role of these bacteria in the pathogenesis of the disease is uncertain, their accurate identification is important for epidemiologic and treatment purposes. therefore, the aims of this study were: (1) to identify these bacteria in patients with bronchiectasis without cystic fibrosis using the protected catheter brush (pcb) in order to avoid oropharyngeal contamination, and (2) to compare the results ...19892912333
coaggregation of black-pigmented bacteroides species with other oral bacteria.coaggregation of bacteroides gingivalis and other black-pigmented bacteroides with several oral bacteria was studied with "reagent" strains specially prepared by methods that have been described previously. b. gingivalis coaggregated with veillonella, capnocytophaga and actinomyces spp., but not with any streptococcus spp. coaggregation of b. gingivalis with other bacteria was inhibited and reversed by lactose. of the asaccharolytic black-pigmented bacteroides, only b. gingivalis demonstrated an ...19892913312
the carboxyltransferase activity of the sodium-ion-translocating methylmalonyl-coa decarboxylase of veillonella alcalescens.methylmalonyl-coa decarboxylase of veillonella alcalescens catalyzed the isotopic exchange between methylmalonyl-coa and [1-14c]propionyl-coa or between malonyl-coa and [1-14c]acetyl-coa. the exchange was independent of sodium ions and was abolished by avidin. the enzyme also catalyzed the carboxyl transfer reaction from methylmalonyl-coa to acetyl-coa yielding propionyl-coa and malonyl-coa, and vice versa. the beta subunit was dissociated from methylmalonyl-coa decarboxylase by prolonged washin ...19892920730
microbial changes associated with advancing periodontitis in str/n mice. 19852933502
serological investigation of various forms of inflammatory periodontitis. 19862942664
selective suppression of the anaerobic oropharyngeal microflora with local metronidazole.anaerobic microorganisms of the normal oropharyngeal flora have been shown to be the main pathogens in orofacial infections of odontogenic origin. reduction of the density of anaerobes in the oral cavity as a prophylactic measure before oral surgery may be a rational way to reduce the frequency of post-operative infections. this report describes a local antibiotic regimen that can reduce anaerobic pathogens in the oral cavity. over a period of 7 days, 10 healthy individuals applied locally 1.5-2 ...19872948544
bacterial antigens induce collagenase and prostaglandin e2 synthesis in human gingival fibroblasts through a primary effect on circulating mononuclear cells.our previous work suggests that one mechanism through which connective tissue breakdown might occur in periodontal diseases is the production of metalloproteinases, including collagenase, by gingival fibroblasts. in this study we investigated whether highly purified preparations of lipopolysaccharide (lps) and lipoteichoic acid (lta) from a number of putative periodontal pathogens could induce monolayer cultures of human gingival fibroblasts to synthesize collagenase and prostaglandin e2. using ...19873040590
[in vitro activity of an ofloxacin-metronidazole combination against anaerobic bacteria. kinetics of the action of metronidazole against bacteroides fragilis].the activity of metronidazole combined with ofloxacin was investigated by the checkerboard method in liquid medium against 60 obligate anaerobes. the bacteriostatic effect of the combination was assessed by calculating the fic index. two metronidazole resistant strains of propionibacterium acnes (mic greater than 32 mg/l) were inhibited by 0.125 mg/l of this former antibiotic in presence of an ofloxacin concentration equal to half the value of the mic. on the 58 other anaerobic strains, the comb ...19883043345
phage-receptor on the cell wall of veillonella rodentium.veillonellophage n2 prevented from adsorbing to veillonella rodentium atcc 17743 cells treated with polymyxin b, and also to lipopolysaccharides (lpss) of the host cells treated with antibiotics. therefore, these results indicate that receptor to phage n2 is cell wall lpss. the lpss of v. rodentium atcc 17743 cells as receptor were characterized. lipid a and total carbohydrate accounted for approximately 40% of the weight of the lipopolysaccharide complex. heptose and 2-keto-3-deoxyoctonate were ...19883048203
[subgingival anaerobic flora in periodontal disease in adults]. 19883050435
comparison of the antimicrobial capability of an abrasive paste and chemical-soak denture cleaners.the objective of this research was to compare the ability of the two most popular methods for denture cleaning to remove plaque microorganisms from dentures. dentu-creme abrasive denture paste and efferdent alkaline peroxide denture-cleanser soak were selected for study. two trials were completed in which these materials were used alone and in combination along with a no-treatment control to determine the level of recoverable plaque bacteria from removable dentures. plaque was allowed to accumul ...19883054068
anaerobic infections in the head and neck region.anaerobic bacteria form the predominant flora of the oral cavity, outnumbering facultative organisms by 10-1,000: 1. the type of anaerobic bacteria and their concentration depend on the anatomical site and the degree of anaerobiosis in the different sites in the mouth. three groups of anaerobic bacteria inhabit the oral cavity; the strict anaerobes, the moderate anaerobes, and the microaerophilic group of organisms. the majority of anaerobic bacterial infections occurring in the region of the mo ...19883074469
production and degradation of formate by veillonella dispar atcc 17745.under strictly anaerobic conditions, the resting cells of v. dispar atcc 17745 produced formate as well as acetate and propionate from pyruvate or from lactate. pyruvate formate-lyase activity was found when the activity was assayed under strictly anaerobic conditions. under aerobic conditions, however, the resting cells did not produce formate from pyruvate or from lactate, though the cells actively metabolized pyruvate or lactate (mainly to acetate). this was ascribed to pyruvate formate-lyase ...19863086409
the effect of lowering the ph on the composition and metabolism of a community of nine oral bacteria grown in a chemostat.nine oral bacteria, associated with both healthy and diseased sites in the mouth, were grown at d = 0.05 h-1 (mean generation time 13.9 h) in a glucose-limited chemostat. after an initial period of steady-state growth at ph 7.0, ph control was discontinued. the ph then decreased until it stabilized at ph 4.1 after 9 d (16 generations), while the eh rose from -165 mv to +160 mv. the lowering in ph resulted in the composition and metabolism of the flora being altered and in increased bacterial agg ...19863095488
l-serine enhances the anaerobic lactate metabolism of veillonella dispar atcc 17745.under anaerobic conditions, the rate of metabolism of lactate by starved resting cells of veillonella dispar atcc 17745 was very low. because pyruvate was metabolized well by the starved cells, oxidation of lactate to pyruvate, which is the first step of the lactate metabolism, must have been limited in the cells. in the starved cells, the levels of the metabolic intermediates, oxalacetate or fumarate, of which reductions to malate or to succinate could be coupled with lactate oxidation to pyruv ...19873114345
nitrite accumulation during anaerobic nitrate reduction by binary suspensions of bacteria isolated from the achlorhydric stomach.binary suspensions of bacteria isolated from the gastric juice of achlorhydric patients were used to determine conditions which favour nitrite accumulation during nitrate reduction. suspensions of veillonella parvula and haemophilus parainfluenzae accumulated nitrite during nitrate reduction in the absence of nitrite-reducing neisseria subflava or streptococcus sanguis. the maximum concentration of nitrite that transiently accumulated decreased predictably as the ratio of nitrite-removing bacter ...19873117970
rapid isolation of dna from actinomyces.dna could not be quickly extracted from members of the genus actinomyces by the usual methods of lysis. treatment of 7 different actinomyces cells with lysozyme and achromopeptidase, both 5 mg/g wet cells, for 2 h, followed by sds (0.2%), proteinase k (5 mg/g wet cells) and edta (lmm) for 1 h, lysed the cells. the yield obtained in one day was 337 micrograms per 200 mg of bacterial cells. the treatment was also found to work effectively on strains belonging to veillonella, staphylococcus, fusoba ...19873125848
production of volatile fatty acids as a result of bacterial interactions in the cecum of gnotobiotic rats and chickens fed a lactose-containing diet.volatile fatty acid (vfa) productions from lactose and lactate by a clostridium butyricum and a veillonella alcalescens strain, alone or in combination with a lactobacillus acidophilus strain, were determined both in vitro in culture media and in vivo in the ceca of gnotobiotic animals. gnotobiotic rats, which possess intestinal lactase, and chickens, which are devoid of it, were used. both animal species were fed a diet containing 4% lactose. the in vitro results showed that the c. butyricum st ...19883148988
bacterial involvement in denture-induced stomatitis.denture and mucosal plaque samples were collected from eight full-denture wearers of whom four suffered from denture-induced stomatitis (dis). cultures were made, and a proportional identification to species level was carried out of bacteria and yeasts. an inventory was made of the predominant flora. results showed that the predominant microflora of both groups, both on dentures and the denture-bearing mucosa, consisted mainly of gram-positive bacteria. differences in the proportions of cocci we ...19883166009
coaggregation properties of human oral veillonella spp.: relationship to colonization site and oral ecology.the primary habitats of oral veillonellae are the tongue, dental plaque, and the buccal mucosa. isolates were obtained from each habitat and tested for coaggregation with a battery of other oral bacterial strains. all 59 tongue isolates tested for coaggregation were veillonella atypica or veillonella dispar. all but one of them coaggregated with strains of streptococcus salivarius, a predominant inhabitant of the tongue surface but not subgingival dental plaque. these tongue isolates were unable ...19883178207
tetracycline resistance and tetm in oral anaerobic bacteria and neisseria perflava-n. sicca.tetracycline-resistant organisms isolated from six patients with periodontal disease included bacteroides spp., eubacterium spp., fusobacterium nucleatum, neisseria perflava-n. sicca, peptostreptococcus anaerobius, veillonella parvula, and facultative streptococci. all but the bacteroides spp. and eubacterium spp. hybridized with the tetm determinant. an additional 417 bacterial strains were screened, and 4% of both the oral streptococci and the fusobacterium spp. hybridized with the tetm probe.19883190205
sextuplibacterial endocarditis: a new world's record? 19883201306
fermentation products, amino acid utilization, maintenance energies and growth yields for the fibrillar streptococcus salivarius hb and a non-fibrillar mutant hb-b grown in continuous culture under glucose limitation.the fibrillar strain streptococcus salivarius hb and a non-fibrillar mutant, strain hb-b, were grown in a defined medium under glucose limitation in a chemostat. fermentation balances were produced for both strains in batch culture and at growth rates between 0.1/h and 1.1/h. in batch culture both strains fermented glucose to lactate, but in continuous culture glucose was fermented to formate, acetate and ethanol with increasing amounts of lactate as the growth rate was increased. lactate never ...19883204071
monobacterial and mixed bacterial plaques of streptococcus mutans and veillonella alcalescens in an artificial mouth: development, metabolism, and effect on human dental enamel.to gain greater understanding of the role of streptococcus mutans and veillonella in the caries process, studies of both aerobically and anaerobically grown plaques of s. mutans c67-1 and v. alcalescens v-1 on human enamel slabs were carried out in an artificial mouth. plaque development, acid production, and demineralization were measured. early plaque development of monobacterial and mixed bacterial plaques started from randomly adhering cells on day 1 to confluent multilayered microcolonies o ...19883214847
the presence of cytochromes in oral anaerobic bacteria, especially in bacteroides oralis. 19883255150
the long-term effects of daily rinsing with stannous fluoride or sodium fluoride on bacteria in dental plaque. 19883268790
[serotypes and antibiotic susceptibility patterns of veillonella spp. strains isolated from oral cavity of an individual]. 19883271905
the predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases.subgingival plaque samples were taken from active and inactive lesions in 33 subjects exhibiting active destructive periodontal diseases. active diseased sites were those which showed a significant loss of attachment within a 2-month interval as computed by the "tolerance method". the predominant cultivable species from 100 active sites were compared with those found in 150 inactive sites of comparable pocket depth and attachment level loss. among the 33 subjects, w. recta, b. intermedius, f. nu ...19883292595
inhibitory and cidal antimicrobial actions of electrically generated silver ions.one promising alternative to antibiotics in the treatment of localized infections is the generation of antimicrobial silver ions by the use of low intensity direct current from a pure silver anode implanted at the site of an infection. this study investigates the in vitro bacteriostatic and bactericidal properties of this system on a variety of organisms.19873305838
uv red fluorescence of veillonella spp.a total of 34 clinical isolates and 7 type strains of veillonella spp. were tested for their ability to fluoresce on various culture media. fluorescence was medium dependent and varied among the species. scanning absorption spectrophotometry of culture extracts showed that the absorption spectrum of the fluorescent pigment is typical of a metal-free porphyrin.19883343333
[sensitivity to an amoxicillin-clavulanic acid combination, of deep tonsillar flora isolated in chronic tonsillitis].deep tonsillar flora were identified in tonsils removed from 48 patients, children and adults, with chronic tonsillitis and recurrent sore throats or obstructive hypertrophy. most specimens (38/48) cultured multiple germs (2 to 5 different species) with aerobic and anaerobic gram+ and gram-forms, some being beta-lactamase producers. of the total of 135 strains isolated, 104 were aerobic and 31 anaerobic. the species of aerobic germs most frequently isolated were: apart from alpha-hemolytic strep ...19883369805
the synergistic effect of cefotaxime and desacetylcefotaxime against clinical isolates of anaerobic bacteria.the synergistic interaction of cefotaxime and desacetylcefotaxime against 187 clinically significant anaerobic organisms was investigated. fusobacterium nucleatum, actinomyces odontolyticus, propionibacteria, lactobacilli, peptostreptococci, streptococcus intermedius and veillonella were sensitive to cefotaxime. both eubacterium lentum and streptococcus morbillorum were resistant. the susceptibility of the clostridia varied from 0.125 to greater than 256 mg/l; only 20% of species demonstrated sy ...19883396488
microbial flora of surface versus core tonsillar cultures in recurrent tonsillitis in children.surface and 'core' tonsillar specimens were collected from 60 children, between 2 and 14 years of age, suffering from recurrent tonsillitis, in order to establish the reliability of surface tonsillar swabbing technique and to define the most frequently involved aerobic and anaerobic microorganisms. from the qualitative point of view, the same mixed aerobic and anaerobic flora were obtained in both samples thus demonstrating the reliability of the surface swabbing technique. from the quantitative ...19883397235
microbiological and clinical effects of chlorhexidine digluconate and hydrogen peroxide mouthrinses on developing plaque and gingivitis.while the ability of chlorhexidine (chx) to prevent plaque formation and inhibit the development of gingivitis has been well documented in the literature, the therapeutic value of hydrogen peroxide (h2o2) in preventing gingivitis is in dispute. the purpose of this study was to compare the clinical and microbiological effects of an established therapeutic agent, such as chlorhexidine with that of h2o2 in the experimental gingivitis model. following a period of stringent oral hygiene, 32 subjects ...19883422246
interactions between rumen amylolytic and lactate-utilizing bacteria in growth on starch.the growth and metabolism of the rumen amylolytic bacteria streptococcus bovis, butyrivibrio fibrisolvens and bacteroides ruminicola, growing in pure cultures and co-cultures with the rumen lactilytic bacteria megasphaera elsdenii and veillonella alcalescens were followed. the interaction of amylolytic bacteria with v. alcalescens represents a simple food chain. the interaction with m. elsdenii is more complex, since there is a simultaneous competition for products of the starch degradation.19873429358
immune suppression induced by gram-negative organisms in patients with chronic inflammatory periodontal disease. 19863460762
[indirect immunohistochemical test of the endotoxin of veillonella alcalescens and of peptidoglycans in the root cementum of periodontally diseased teeth]. 19863461943
a bacteriological study of rampant caries in children.we undertook a microbiological study, in children, of dental plaque from sound surfaces or associated with rampant caries, both black-colored and unstained. improved selective media allowed for the enumeration of bacteria belonging to specific genera or species present in plaque samples. a nearly similar bacterial distribution was found in both types of rampant caries. aciduric flora, streptococcus mutans, veillonella, and lactobacillus predominated in plaque over the lesions, whereas extracellu ...19873476538
phenytoin and 5-(p-hydroxyphenyl)-5-phenylhydantoin do not alter the effects of bacterial and amplified plaque extracts on cultures of fibroblasts from normal and overgrown gingivae.local irritation of gingival tissue by plaque is among the factors which affect development of gingival overgrowth in patients undergoing chronic phenytoin (pht) therapy. variability in the cytotoxicity of plaque components or of plaque substances plus pht and/or its metabolites toward gingival fibroblasts may relate to whether gingival overgrowth forms in a particular patient. fibroblasts from healthy and overgrown gingivae were incubated with (a) pht and its major human metabolite, 5-(p-hydrox ...19873476609
arylaminopeptidase activities of oral bacteria.protease and peptidase enzymes are thought to play a role in the virulence of many oral organisms, especially those associated with periodontal diseases. in order to evaluate the peptidases of periodontopathogens, we compared the arylaminopeptidase activities of bacteroides gingivalis with those of other oral and non-oral bacteria. sixty-three bacterial strains representing the prominent cultivable organisms in human periodontal pockets were tested, including representatives of the black-pigment ...19863478393
serum igg antibodies to lipopolysaccharides in various forms of periodontal disease in man.serum igg antibody titres to lipopolysaccharides (lps) from two strains of actinobacillus actinomycetemcomitans were significantly elevated in juvenile periodontitis compared with other types of periodontal disease and with controls (p less than 0.05). the highest antibody titres to bacteroides gingivalis lps were in juvenile periodontitis, but this difference was significant only against the control group (p less than 0.01). in adult mild periodontitis there were higher antibody levels to lps f ...19863479058
penicillin resistance in the subgingival microbiota associated with adult periodontitis.in this investigation, the penicillin-resistant and beta-lactamase-producing subgingival microbiota associated with adult periodontitis was identified, and the impact of a recent exposure to penicillin on the recovery of resistant organisms from this microbiota was assessed. subjects with adult periodontitis were examined clinically and microbiologically. twenty-one subjects had a documented history of penicillin therapy within the previous 6 months whereas an additional 21 subjects had no histo ...19863486882
[cellular immunity in periodontal disease]. 19873502633
[nitrite reductase in soluble cytoplasmic fraction and particulate membrane-bound fraction from veillonella]. 19873505784
gingivitis.gingivitis is caused by substances derived from microbial plaque accumulating at or near the gingival sulcus; all other suspected local and systemic etiologic factors either enhance plaque accumulation or retention, or enhance the susceptibility of the gingival tissue to microbial attack. microbial species specifically associated with gingival health include streptococcus sanguis 1, s. d-7, and fusobacterium naviforme. bacteria involved in the etiology of gingivitis include specific species of s ...19863522644
effect of beta-lactam prodrugs on human intestinal microflora.the ampicillin prodrugs bacampicillin, pivampicillin, and talampicillin, the mecillinam prodrug pivmecillinam and the sulbactam prodrug sulbactam pivoxil all have a greatly improved oral availability compared to the parent drug. they show no antibacterial activity themselves until transformed into active drugs after absorption. this double advantage makes them less likely to influence the intestinal microbial ecosystem. ampicillin has been reported to cause marked changes in the colon microflora ...19863547627
putrescine and cadaverine are constituents of peptidoglycan in veillonella alcalescens and veillonella parvula.veillonella alcalescens atcc 17745, a strictly anaerobic, gram-negative small coccus, requires putrescine or cadaverine for growth (m. b. ritchey, and e. a. delwiche, j. bacteriol. 124:1213-1219, 1975). both putrescine and cadaverine were demonstrated to be incorporated exclusively into the peptidoglycan layer of v. alcalescens atcc 17745. v. parvula gai 0574 also proved to contain putrescine as a component of peptidoglycan. the primary chemical structure of the peptidoglycan common to the two v ...19873584075
heparinase production by anaerobic bacteria.the production of heparinase by a wide range of anaerobic bacteria isolated from clinical specimens was investigated. none of the 29 strains of bacteroides fragilis produced heparinase. of 62 other bacteroides tested, only two of four strains of b ovatus, two of three strains of b thetaiotaomicron, and two of four strains of b uniformis were heparinase producers. none of the 48 strains of fusobacteria or seven strains of veillonella produced heparinase. the anaerobic cocci (19 peptococci and sev ...19873584480
stereochemistry of the methylmalonyl-coa decarboxylation reaction.the steric course of the decarboxylation of (s)-methylmalonyl-coa to propionyl-coa, catalyzed by the biotin-dependent sodium pump methylmalonyl-coa decarboxylase of veillonella alcalescens was determined. the decarboxylation of (s)-methylmalonyl-coa in 3h2o yielded (r)-[2-3h]propionyl-coa; and the decarboxylation of (s)-[2-3h]methylmalonyl-coa in h2o produced (s)-[2-3h]propionyl-coa. the results demonstrate retention of configuration during the decarboxylation reaction. the substrate stereochemi ...19873609308
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