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[lysogenicity and lysosensitivity of strains of salmonella cholerae-suis and salmonella typhi-suis]. 19695398929
[salmonella choleraesuis as the cause of paranephritic abscess]. 19705438751
the chemotherapy of experimental salmonella choleraesuis infetion in mice. 19705452463
porcine salmonellosis. ii. production of the generalized shwartzman reaction by intravenous injections of disintegrated cells of salmonella cholerae-suis. 19705472124
pyogenic meningitis caused by salmonella cholerae-suis var. kunzendorf. 19705506192
[salmonella cholerae suis var. america as a cause of sepsis with joint involvement]. 19705535378
the sensitivity of salmonella choleraesuis to sodium sulphadiazine. 19715575484
[infection of the ankle-joint by salmonella cholerae suis var. kunzendorf]. 19675585454
[some data concerning salmonellosis in poland in the postwar period, with special reference to salmonella cholerae suis]. 19685669052
[experience obtained from the use of formol-adsorbat vaccines against salmonella cholerae-suis in pig stocks]. 19685692747
[bone complication caused by salmonella cholerae suis var. kunzendorf in an 18-month-old boy]. 19685701269
[bacterial endocarditis caused by salmonella cholerae suis, kunzendorf type, with fatal outcome]. 19685735144
2-amino-5-(1-methyl-5-nitro-2-imidazolyl)-1,3,4-thiadiazole, a new antimicrobial agent. v. therapy of an experimental salmonella choleraesuis infection iswine. 19685735401
[exudative pericarditis caused by salmonella cholerae suis]. 19685749000
association of increased recipient ability for r factors and reduced virulence among variants of salmonella choleraesuis var. kunzendorf. 19695773040
salmonella choleraesuis infections in man: a report of 19 cases and a critical literature review. 19695782191
immunoglobulin development in sera of pigs injected with killed suspensions of salmonella choleraesuis. 19695813746
mycotic aneurysm of the abdominal aorta: a case due to salmonella choleraesuis var. kunzendorf. 19695818697
[osteomyelitis in infants, caused by salmonella cholerae suis]. 19655880205
experimental vaccination of pigs with avirulent rough strains of salmonella choleraesuis. 19655892483
[salmonella cholerae suis resistance to furazolidone and some antibiotics]. 19655896141
[severe case of septicemia caused by salmonella cholerae suis var.kunzendorf]. 19665913447
[demonstration of salmonella cholerae suis in swine from pig farms in relation to bacteriologic meat inspection]. 19665973886
[cephalothin in sepsis caused by salmonella cholerae suis]. 19665994694
[contribution on salmonella cholerae suis infections]. 19666009687
observations on experimental oral infection with salmonella dublin in calves and salmonella choleraesuis in pigs. 19676029752
trial with salmonella cholerae-suis vaccine for pigs. 19676067660
[from the casuistry of salmonellosis (salmonella choleraesuis)]. 19676068568
septicaemia due to salmonella choleraesuis. 19676072132
pneumonia due to salmonella choleraesuis in infants in papua new guinea. 19836136739
the antigenic relationship between candida and salmonella o:6(2), o:7 antigens.the immune sera for candida albicans, c. guilliermondii and c. tropicalis agglutinated salmonella cholerae-suis (o:6(2),7). the immune serum for s. cholerae-suis agglutinated c. albicans, c. guilliermondii and c. tropicalis. absorption and agglutination cross tests demonstrated common antigenic factor between above mentioned yeasts and salmonella o:6(2), o:7 antigens.19806158816
the o antigenic relationships amongst dekkera, hanseniaspora, escherichia coli o:75, salmonella cholerae-suis and salmonella aberdeen.the immune sera for dekkera bruxellensis, d. intermedia and hanseniaspora osmophila agglutinated salmonella cholerae-suis (o:62,7) and vice versa. the immune serum for h. osmophila also agglutinated e. coli (o:75), s. aberdeen (o:11) and vice versa. cross agglutination and absorption tests demonstrated common antigenic factor(s) between above mentioned yeasts and salmonella o:62, o:7 antigens. h. osmophila also showed a common antigenic factor(s) with e. coli (o:75) and s. aberdeen (o:11).19806162296
salmonella choleraesuis endocarditis. 19846589489
sensitivity of smooth salmonella cholerae-suis var kunzendorf to killing by porcine polymorphonuclear neutrophils and its relation to mouse virulence.twenty-two selected strains of salmonella cholerae-suis var kunzendorf were evaluated for their in vitro susceptibility to the actions of porcine peripheral blood polymorphonuclear neutrophils (pmns) and/or antibody (ab). strains were divided into 2 groups based on their previously reported sensitivity or resistance to antibody-complement (ab-c) lysis. strains resistant to ab-c lysis resisted killing by pmns to a greater extent than strains susceptible to ab-c lysis. in the absence of pmns, dilu ...19836612987
mycotic aortic aneurysm. a complication of campylobacter fetus septicemia.the first surviving case, to our knowledge, of a campylobacter fetus mycotic aortic aneurysm is reported. bacteremia and an ileofemoral thrombophlebitis preceded the development of the infected aneurysm, reconfirming the vascular tropism of this organism. the clinical similarity with infections caused by salmonella choleraesuis is illustrated by this case. the full recovery of our patient attests to the efficacy of extralanatomic bypass combined with long-term antibiotic therapy in the treatment ...19836687525
[pleural empyema caused by salmonella cholerae suis in a patient with breast cancer in the stage of metastatic dissemination].a pleural localisation of salmonella cholerae suis infection is described in a patient with breast carcinoma. the evolution of neoplastic disease seems to be more slow after salmonellosis. on the basis of this case the most important mechanisms between immunity and tumors are discussed.19846728272
introduction and reisolation of selected gram-negative bacteria from fermented edible wastes.a lactobacillus fermentation process, using edible food wastes, was tested for its ability to eliminate selected bacterial pathogens. this fermentation process converts food wastes into a feed ingredient for animal consumption. six gram-negative bacterial pathogens of potential zoonotic importance were tested. these experimental organisms were: salmonella enteritidis serovar typhimurium, s enteritidis serovar anatum, s cholerae-suis, yersinia enterocolitica, y pseudotuberculosis, and pasteurella ...19816794391
sensitivity of smooth salmonella cholerae-suis var kunzendorf field strains to antibody complement under various conditions.seventy-two field strains of smooth salmonella cholerae-suis var kunzendorf were obtained from outbreaks of swine parathyroid. the field strains were examined for susceptibility to killing by antibody-complement (ab-c) after they were exposed to tris-edta, lysozyme, or saline solution. considerable differences were found in susceptibility to killing by ab-c in the strain population. some strains were highly sensitive to ab-c under all experimental conditions, whereas others became sensitive only ...19826808877
[advantages of using semi-solid agar medium for the isolation of salmonellae].a method of salmonellae rapid isolation on semi-solid agar medium in glass u-shaped tubes was tested and the results were compared with those obtained by using the selective multiplication medium after rappaport and kauffmann medium with sub-cultures on agars with brilliant green and sodium desoxycholate. this method was verified by employing the collection strains of selected serotypes of salmonellae, e. coli collection strains and microorganisms of the genus proteus. the described method was u ...19826812264
salmonella infections of the abdominal aorta.endovascular infection of the atherosclerotic aorta is a substantial risk in patients older than 50 years of age who have bacteremia due to salmonella. until recently, the resultant salmonella aortitis was uniformly fatal. two patients who were cured are described, and the characteristics of 10 other reported long-term survivors are given. this recent improvement in prognosis may have been related to (1) earlier diagnosis, (2) improved surgical management (axillofemoral bypass graft), and/or (3) ...19836844803
salmonella choleraesuis encephalopathy. 19806932598
antibacterial antibodies in swine colostrum.antibacterial antibody activities were examined in 138 samples of swine colostrum by o-agglutinin test with serotypes of escherichia coli (o-6, 0-45, 0-81, 0-83, 0-115, 0-138, o-139, and o-126), salmonella typhimurium, and salmonella cholerae suis. the mean agglutinin titer of e coli o-83 was the highest; e coli o-115 was next highest. the mean agglutinin titer of s cholerae suis was lowest; s typhimurium was next to lowest. correlation coefficients among these o-agglutinin titers were between 0 ...19806989301
pyridoquinoxaline n-oxides. 2. synthesis and antibacterial activity of tricyclic lactams.a series of novel 3,4-dihydropyrido[3,4-b]quinoxalin-1(2h)-one 5,10-dioxides was synthesized using an intramolecular amidation reaction. the lactams were screened in vitro and in vivo against salmonella choleraesuis, pasteurella multocida, and escherichia coli. an n-methyl analogue was the most potent member of this series, with antibacterial activity comparable to that of the commercially important quinoxaline 1,4-dioxide carbadox.19827108904
[various biochemical properties of salmonella strains isolated from animal food products].studied were a total of 488 salmonella strains isolated from food products and washings from productional surfaces for the presence of variants, with the use of tests of producing gas from glucose and hydrogen sulfide and the coincidence of the two properties. it was found that 7 species only had biochemical variants. most frequent were the variants with s. cholerae suis. biochemical variants were found in 24.79 per cent of the typed salmonellae, these of the s. cholerae suis species being 19.26 ...19827135819
salmonella thyroiditis, apathetic thyrotoxicosis, and follicular carcinoma in a laotian woman.a 49-year-old laotian woman presented with apathetic hyperthyroidism and suppurative thyroiditis from salmonella cholerae-suis occurring in a multinodular gland with a large right-sided mass. apathetic hyperthyroidism in this age group is uncommon and salmonella thyroiditis has been infrequently described. surgical removal of the mass which had effectively concentrated radioactive iodine cured the hyperthyroidism and permitted the remaining suppressed normal tissue to regain function. pathologic ...19827139541
porcine proliferative enteritis: experimentally induced disease in cesarean-derived colostrum-deprived pigs.the hypotheses that porcine proliferative enteritis is an infectious disease and that campylobacter sputorum subsp mucosalis (csm) is involved in the development of this disease were experimentally tested. three experiments were conducted with 10-week-old, cesarean-derived colostrum-deprived pigs. of 22 pigs given homogenized mucosal scrapings (crude inocula) intragastrically, 15 had gross and/or microscopic lesions of proliferative enteritis. of 10 pigs inoculated with cultures of both csm and ...19827149408
[salmonella cholerae-suis infections]. 19827162804
observations on the occurrence of salmonella cholerae-suis and other salmonellas in two herds of feeder pigs.modified brilliant green agar (bga), muller-kauffmann tetrathionate, rappaport's and selenite f broths were compared for their efficiency in isolating salmonellas from pigs and their excreta. it was concluded that bga and rappaport's broth were the media of choice. where searches were made for salmonella cholerae-suis alone, the use of a trehalose mcconkey agar provided a rapid method of differentiating s. cholerae-suis, which does not ferment trehalose, from the majority of other salmonellas, w ...19817240737
cephamycin c treatment of induced swine salmonellosis.weanling pigs in groups of 12 were infected orally with salmonella choleraesuis and were treated intramuscularly with doses of cephamycin c ranging from 12.5 to 337.5 mg twice daily for 10 days beginning 1 day postinoculation. pigs in two other infected groups either received 300 mg of tetracycline orally on a similar schedule or served as nonmedicated controls. optimal responses to cephamycin c were achieved at a twice daily dose of 112.5 mg. with this regimen, the febrile response was signific ...19817247378
pig neutrophil adherence in experimentally induced salmonellosis.in vitro neutrophil adherence was characterized in healthy newly weaned pigs following experimentally induced infection with salmonella choleraesuis, a typhimurium, and s anatum. the adherence of pig neutrophils to nylon fiber columns was shown to be reproducible on a day-to-day basis. neutrophil adherence was significantly increased in a group of pigs at 6, 30, and 48 hours after s choleraesuis was inoculated (orally). neutrophil adherence was not significantly altered in groups of pigs inocula ...19817271046
[inflammation of the hip joint and femur head caused by salmonella cholerae suis]. 19817313138
[reproduction of salmonellosis in swine by aerosol infection].four 36-day-old pigs were infected through aerosol with salmonella cholerae suis var. america, and one pig was injected with same pathogen intravenously as a control, all animals being treated in a chamber. the temperature and the other clinical signs were followed up. rectal samples and hemocultures were investigated for salmonella organisms. on the 4th, 8h, 14th, and 25th day a pig was killed for bacteriologic and histopathologic investigations of the viscera. as early as the 48th hour there w ...19817324376
salmonella choleraesuis septicemia in calf. 19817340933
increased susceptibility of lead-exposed swine to salmonella choleraesuis var kunzendorf.the effect of oral subclinical lead exposure on the susceptibility of swine to salmonella choleraesuis var kunzendorf was studied in two experiments. in experiment 1, 18 pigs were exposed to lead acetate and 18 pigs were exposed to sodium acetate for 1, 2, or 3 weeks and then challenge exposed intraperitoneally. in experiment 2, 8 pigs were exposed to lead chloride for 2 weeks, 8 pigs were exposed to lead chloride for 1 week, and 8 pigs were exposed to sodium chloride for 2 weeks. these pigs wer ...19807406264
[suisaloral "dessau"--a salmonella cholerae suis live vaccine for oral, parenteral and combined applications].an account is given of all immune preparations which had been available by summer 1976 and their uses in public disease control, with reference being made to the epizootiological role and detrimental effects of salmonella cholerae suis (s. c.-s.). the need for using live salmonella strains to test their immunogenic properties is derived from a thorough assessment of inactivated s.-c.-s.-vaccine, salmoporc "dessau", which had been used in the past. salmonellae are pathogens of zoo-anthroponoses b ...19807416899
[diagnosis of some yeasts in metschnikowia genus with the aid of salmonella cholerae-suis o agglutinating serum (author's transl)].in this paper a common antigenic factor among salmonella choleraesuis 0 antigen and standard metschnikowia bicuspidata var. bicuspidata and m. pulcherrima strains is shown. this common factor was not present in m. bicuspidata var. australis, m. bicuspidata var. california, m. krissii and m. reukaufii strains. m. bicuspidata var. chathamia and m. zobellii showed agglutination in the previous experiments. according to these results, the use of s. choleraesuis 0:6,7 agglutinating serum for slide an ...19807464579
serologic studies of experimentally induced salmonella choleraesuis var kunzendorf infection in pigs.two indirect elisa containing outer membrane protein (omp) and lipopolysaccharide (lps) antigens from a field isolate of salmonella choleraesuis var kunzendorf were developed and evaluated in experimentally infected and uninfected control pigs. experimentally induced infection with s choleraesuis was successfully established in 10 pigs by oral inoculation with 10(8) organisms, and 3 pigs died of clinical salmonellosis at postinoculation (pi) weeks 1, 2, and 4. swab specimens from tonsils, nostri ...19957486393
comparison of mics of ceftiofur and other antimicrobial agents against bacterial pathogens of swine from the united states, canada, and denmark.the mics of ceftiofur and other antimicrobial agents, tested for comparison, for 515 bacterial isolates of pigs from the united states, canada, and denmark with various diseases were compared. the organisms tested included actinobacillus pleuropneumoniae, escherichia coli, pasteurella multocida, salmonella choleraesuis, salmonella typhimurium, streptococcus suis, streptococcus dysgalactiae subsp. equisimilis, streptococcus equi subsp. equi, and streptococcus equi subsp. zooepidemicus. in additio ...19957494042
characterization of the rfc region of shigella flexneri.the o antigen of the shigella flexneri lipopolysaccharide (lps) is an important virulence determinant and immunogen. we have isolated s. flexneri mutants which produce a semi-rough lps by using an o-antigen-specific phage, sf6c. western immunoblotting was used to show that the lps produced by the semi-rough mutants contained only one o-antigen repeat unit. thus, the mutants are deficient in production of the o-antigen polymerase and were termed rfc mutants. complementation experiments were used ...19947507920
a plasmid-encoded rfbo:54 gene cluster is required for biosynthesis of the o:54 antigen in salmonella enterica serovar borreze.previous studies demonstrated that the presence of a 7-8 kb plasmid is correlated with expression of the lipopolysaccharide (lps) o:54 antigen in several salmonella enterica serovars. in this study, a 6.7 kb plasmid from a field isolate of s. enterica serovar borreze was shown to encode enzymes responsible for the synthesis of the o:54 polysaccharide. curing the plasmid results in simultaneous loss of smooth o-polysaccharide-substituted lps molecules and o:54 serotype. sds-page analysis of other ...19947512186
cloning and analysis of duplicated rfbm and rfbk genes involved in the formation of gdp-mannose in escherichia coli o9:k30 and participation of rfb genes in the synthesis of the group i k30 capsular polysaccharide.the rfbo9 gene cluster, which is responsible for the synthesis of the lipopolysaccharide o9 antigen, was cloned from escherichia coli o9:k30. the gnd gene, encoding 6-phosphogluconate dehydrogenase, was identified adjacent to the rfbo9 cluster, and by dna sequence analysis the gene order gnd-rfbm-rfbk was established. this order differs from that described for other members of the family enterobacteriaceae. nucleotide sequence analysis was used to identify the rfbk and rfbm genes, encoding phosp ...19947515042
genetic analysis of the o-specific lipopolysaccharide biosynthesis region (rfb) of escherichia coli k-12 w3110: identification of genes that confer group 6 specificity to shigella flexneri serotypes y and 4a.we recently reported a novel genetic locus located in the sbcb-his region of the chromosomal map of escherichia coli k-12 which directs the expression of group 6-positive phenotype in shigella flexneri lipopolysaccharide, presumably due to the transfer of o-acetyl groups onto rhamnose residues of the s. flexneri o-specific polysaccharide (z. yao, h. liu, and m. a. valvano, j. bacteriol. 174:7500-7508, 1992). in this study, we identified the genetic region encoding group 6 specificity as part of ...19947517390
involvement of the galactosyl-1-phosphate transferase encoded by the salmonella enterica rfbp gene in o-antigen subunit processing.rfbt of salmonella enterica lt2 was previously thought, together with rfal, to be involved in the ligation of polymerized o antigen to core-lipid a, and three mutants were known. we report the mapping of the mutations to rfbp, the galactosyl-1-phosphate transferase gene, which is now shown to encode a bifunctional protein. the mutations which have the former rfbt phenotype are referred to as rfbp(t). we also show that rfbp(t) mutants are not blocked in the ligation step as previously believed bu ...19947517393
usefulness of molecular genetic markers in the typing of salmonella enterica serovar enteritidis causing a food-borne outbreak.a combination of serotyping-phagetyping and three molecular genetic markers (plasmid analysis, chromosomic dna restriction pattern and ribosomal rna gene restriction pattern or ribotyping) was used in the typing of salmonella enterica causing a food-borne outbreak. the isolates analysed, 29 from stools and eight from foods, belonging to serovar enteritidis-phagetype a, carried a 36-mda plasmid, showed a similar dna restriction pattern and the same ribopattern. these data indicate that only one s ...19947521196
molecular, genetic, and topological characterization of o-antigen chain length regulation in shigella flexneri.the rfb region of shigella flexneri encodes the proteins required to synthesize the o-antigen component of its cell surface lipopolysaccharides (lps). we have previously reported that a region adjacent to rfb was involved in regulating the length distribution of the o-antigen polysaccharide chains (d. f. macpherson et al., mol. microbiol. 5:1491-1499, 1991). the gene responsible has been identified in escherichia coli o75 (called rol [r. a. batchelor et al., j. bacteriol. 173:5699-5704, 1991]) a ...19957532168
transferases of o-antigen biosynthesis in salmonella enterica: dideoxyhexosyltransferases of groups b and c2 and acetyltransferase of group c2.the o antigen is a polymer of oligosaccharide units. o antigens differ in their sugar composition and glycosidic linkages, and genes responsible for o-antigen-specific biosynthesis are grouped in the rfb gene cluster. in this study, we identified two abequosyltransferase genes and an acetyltransferase gene in salmonella enterica groups b and c2 by in vitro assay and identified paratosyl-, tyvelosyl-, and abequosyltransferase genes from s. enterica groups a and d and yersinia pseudotuberculosis s ...19957541787
lateral transfer of rfb genes: a mobilizable cole1-type plasmid carries the rfbo:54 (o:54 antigen biosynthesis) gene cluster from salmonella enterica serovar borreze.plasmid pwq799 is a 6.9-kb plasmid isolated from salmonella enterica serovar borreze. our previous studies have shown that the plasmid contains a functional biosynthetic gene cluster for the expression of the o:54 lipopolysaccharide o-antigen of this serovar. the minimal replicon functions of pwq799 have been defined, and a comparison with nucleotide and protein databases revealed this replicon to be virtually identical to cole1. this is the first report of involvement of cole1-related plasmids ...19957545154
effects of in vivo administration of anti-il-10 monoclonal antibody on the host defence mechanism against murine salmonella infection.interleukin-10 (il-10) is a cytokine that regulates various macrophage functions. to elucidate the involvement of endogenous il-10 in the early stage of murine salmonellosis, we examined the effect of anti-il-10 monoclonal antibody (mab) administration on the host defence mechanism against salmonella choleraesuis infection. the in vivo administration of anti-il-10 mab significantly enhanced host resistance at the early stage of salmonella infection, as assessed by bacterial growth in the periton ...19957558125
hard surface carrier test as a quantitative test of disinfection: a collaborative study.the hard surface carrier test (hsct) recently was proposed as a qualitative test for disinfectant efficacy. a collaborative study of hsct led to a suggested performance standard of < or = 2 or 3 positive carriers out of 60 tested. subsequently, it was discovered that hsct can be used as a quantitative test, because the hsct protocol requires measurement of inoculum level on some carriers. the data allow estimation of the log10 reduction in number of active bacteria. producers, consumers, and pol ...19957580324
sequence and analysis of the o antigen gene (rfb) cluster of escherichia coli o111.the o antigens found in salmonella enterica (se) and escherichia coli (ec) show a great deal of diversity, and only three structures are known to be common to both genera. two of them contain the 3,6-dideoxyheoxse colitose, not found in other serogroups of the two species. the first of these is common to ec o111 and se o:35 (sv adelaide); the other is found in both ec o55 and se o:50 (sv greenside). the genes specific for the synthesis of o antigen are generally located in the rfb gene cluster a ...19957590310
transcriptional regulation and promoter sequence of the spvr gene of virulence plasmid pkdsc50 in salmonella choleraesuis serovar choleraesuis.the transcript of the spvr gene on the virulence plasmid, pkdsc50, of salmonella choleraesuis serovar choleraesuis was detected for the first time by northern blot analysis, and the transcriptional regulation of the spvr gene was investigated. the transcription of the spvr was negatively regulated by spva and spvb, and enhanced at stationary phase under control of a sigma factor rpos (sigma 38). the spvr transcript was 2.4 kilonucleotides in salmonella cells, and deduced to encode spvr and spva, ...19957607404
[study of 2 food poisoning outbreaks in mora (toledo) with the same source of infection].the occurrence of an outbreak needs an immediate investigation, because on it depends the necessary measures to control the spread of the epidemic, and it is also the way of knowing the causal factor. we present the results of the investigation of two food poisoning outbreaks (outbreak a and outbreak b) occurred in mora (toledo). both outbreaks were reported at the same time, the source of infection was the same and the total number of exposed people was 620.19947618037
relationship between evolutionary rate and cellular location among the inv/spa invasion proteins of salmonella enterica.for 21 strains of salmonella enterica, nucleotide sequences were obtained for three invasion genes, spao, spap, and spaq, of the chromosomal inv/spa complex, the products of which form a protein export system required for entry of the bacteria into nonphagocytic host cells. these genes are present in all eight subspecies of the salmonellae, and homologues occur in a variety of other bacteria, including the enteric pathogens shigella and yersinia, in which they are plasmid borne. evolutionary div ...19957638176
bacterial plasminogen receptors: in vitro evidence for a role in degradation of the mammalian extracellular matrix.the potential of bacterium-bound plasmin to degrade mammalian extracellular matrix and to enhance bacterial penetration through basement membrane was assessed with the adherent strain sh401-1 of salmonella enterica serovar typhimurium. typhimurium sh401-1 was able to bind plasminogen and to enhance the tissue-type plasminogen activator-mediated activation of the single-chain plasminogen to the two-chain plasmin. the end product, the enzymatically active, bacterium-bound plasmin activity, was als ...19957642304
mice deficient in gamma delta t cells are resistant to lethal infection with salmonella choleraesuis. 19957642318
endogenous interleukin 10 prevents apoptosis in macrophages during salmonella infection.to elucidate the biological roles of endogenous interleukin 10 (il-10) in macrophage responses during bacterial infection, we examined in vitro effects of neutralizing il-10 by anti-il-10 monoclonal antibodies (mab) on apoptosis of the peritoneal macrophages following salmonella choleraesuis infection. marked increments of tnf-alpha production were observed in the culture supernatant later than 6 h after in vitro culture with anti-il-10 mab. these macrophages succumbed to apoptosis at this stage ...19957646518
phage types of salmonella enterica ssp. enterica serovar typhimurium isolated from production animals and humans in denmark.s. typhimurium is one of the 2 most common salmonella serotypes causing human salmonellosis in denmark. in order to illustrate the significance of different production animals as a source of infection, 1461 isolates were characterized by phage typing. the isolates originated from human patients and from cattle, pigs and poultry. by phage typing the isolates could be separated in 35 different phage types. five types (10, 12, 66, 110 and 135) predominated and comprised 78.8% of the isolates. in hu ...19947676916
identification, expression, and dna sequence of the gdp-mannose biosynthesis genes encoded by the o7 rfb gene cluster of strain vw187 (escherichia coli o7:k1).the o7-specific lipopolysaccharide (lps) in strains of escherichia coli consists of a repeating unit made of galactose, mannose, rhamnose, 4-acetamido-2,6-dideoxyglucose, and n-acetylglucosamine. we have recently cloned and characterized genetically the o7-specific lps biosynthesis region (rfbeco7) of the e. coli o7:k1 strain vw187 (c. l. marolda, j. welsh, l. dafoe, and m. a. valvano, j. bacteriol. 172:3590-3599, 1990). in this study, we localized the gnd gene encoding gluconate-6-phosphate deh ...19937677991
repeat unit polysaccharides of bacteria: a model for polymerization resembling that of ribosomes and fatty acid synthetase, with a novel mechanism for determining chain length.we report the identification and sequence from escherichia coli and salmonella enterica strains of the cld gene, encoding the chain-length determinant (cld) which confers a modal distribution of chain length on the o-antigen component of lipopolysaccharide (lps). the distribution of chain lengths in the absence of this gene fits a model in which as the chain is extended there is a constant probability of 0.165 of transfer of growing chain to lps core, with termination of chain extension. the dat ...19937682279
glycosyl transferases of o-antigen biosynthesis in salmonella enterica: identification and characterization of transferase genes of groups b, c2, and e1.in salmonella enterica, there is a great variety of o antigens, each consisting of a short oligosaccharide (the repeating unit) repeated many times. the o antigens differ in their sugar composition and glycosidic linkages. the genetic determinants of the o antigen are located in an rfb gene cluster, and some, including those of s. enterica o serogroups b, c2, and e1, have been cloned and sequenced. in this study of the glycosyltransferases which form the glycosidic linkages, we identify and char ...19937684736
variation of the rfb gene clusters in salmonella enterica.in order to explore the genetic variation of o antigens of salmonella enterica, we surveyed 164 strains (132 serovars) belonging to 45 serogroups, using 25 mostly single-gene rfb dna probes for colony hybridization. the results revealed that strains within a serogroup have very similar or identical rfb genes. at least three of the four rhamnose genes were detected in all 17 serogroups reported to contain rhamnose, and one or more were detected in three others. the likelihood of being detected de ...19937687601
safety, efficacy, and duration of immunity induced in swine by use of an avirulent live salmonella choleraesuis-containing vaccine.an avirulent live salmonella choleraesuis culture (sc-54) was evaluated for use as an effective vaccine in preventing salmonellosis caused by s. choleraesuis in pigs. eighty-two pigs, 3 to 4 weeks old, were randomly assigned to 1 of 2 treatment groups, which were designated as either vaccinates or controls. after vaccination, all pigs were examined for fecal shedding of s choleraesuis, rectal temperature, and 10 clinical variables. significant difference was not detected between vaccinated and n ...19957695147
phage conversion in salmonella enterica serotype enteritidis: implications for epidemiology.a model system for the study of phage conversion of salmonella enterica serotype enteritidis is reported. temperate phages 1,2,3 and 6 from the phage typing scheme were used to convert several individually recognized phage types into others. phage type 4 was converted to pt8, pt6a to pt4, pt6a to pt7, pt13 to pt13a and pt15 to pt11; some new phage lysis patterns were also detected. this model was used to examine the relationships between phage types within a previously defined clonal lineage, se ...19957705486
diversity and molecular variation among plasmids in salmonella enterica serotype dublin based on restriction enzyme fragmentation pattern analysis.molecular variation within and between plasmids of salmonella enterica serotype dublin was analysed. such variation has been demonstrated in the serotype-specific plasmids (ssp's) of typhimurium and enteritidis. the two aims of this study were to determine the plasmid diversity in a host-adapted serotype and also the incidence of molecular variation in the ssp among strains of dublin using restriction endonuclease fragmentation pattern (refp) analysis with pst1, sma1 and ecorv. sixty-five strain ...19957705487
new extended-spectrum tem-type beta-lactamase from salmonella enterica subsp. enterica isolated in a nosocomial outbreak.a new extended-spectrum beta-lactamase was detected in a lactose-positive salmonella enterica subsp. enterica strain that caused a nosocomial outbreak involving eight patients in a pediatric cardiology unit. this strain showed high levels of resistance to ceftazidime and aztreonam and relatively low levels of resistance to cefotaxime and ceftriaxone. resistance was associated with a conjugative plasmid of 59 kb, which encoded a new beta-lactamase with an isoelectric point of 5.9 that strongly hy ...19957726515
use of ribotyping, is200 typing and plasmid analysis for the identification of salmonella enterica subsp. enterica serovar typhimurium vaccine strain zoosaloral h and its differentiation from wild type strains of the same serovar.fifty salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) isolates obtained from one vaccinated and three non-vaccinated poultry flocks as well as the commercially available vaccine strain zoosaloral h and a s. typhimurium reference strain were characterized genotypically to differentiate between s. typhimurium live vaccine strain zoosaloral h and wild type strains of the same serovar. ribotyping revealed five different patterns one of which exclusively occurred in the vacci ...19947727890
genotypic and phenotypic analysis of salmonella strains associated with an outbreak of equine neonatal salmonellosis.isolates of salmonella choleraesuis serotype ohio (s. ohio) recovered during an outbreak of equine neonatal salmonellosis on a thoroughbred farm were compared with isolates of the same serotype from various animal, feed and environmental sources. biochemical profiles, antimicrobial susceptibility patterns, phage susceptibility, plasmid profiles, restriction endonuclease analysis and ribotyping were used to compare relatedness of the strains. a total of 46 outbreak and non-outbreak associated iso ...19957740753
septicemic salmonellosis in two llamas.at necropsy, salmonella choleraesuis var kunzendorf was recovered from the lungs of a 6-year-old female llama that died within a few days of onset of illness. the llama had had fence-line contact with 40 sows at a farm. salmonella typhimurium was isolated from a blood sample of a 6-day-old male cria that also died after a short illness.19957744668
nucleotide sequence of iaga and iagb genes involved in invasion of hela cells by salmonella enterica subsp. enterica ser. typhi. 19957754225
epidemiological evaluation by pcr ribotyping of sporadic and outbreak-associated strains of salmonella enterica serotype typhimurium.salmonella enterica serotype typhimurium has a very large diffusion worldwide within human and non-human hosts. the simultaneous circulation in the same geographical areas of many bacterial clones requires the use of reliable, reproducible and highly discriminatory typing techniques for epidemiological studies. molecular biological methods, such as plasmid profile analysis, restriction endonuclease digestion of plasmid and chromosomal dna and hybridization-based procedures have proven to be usef ...19957754232
[characteristics of biological properties of salmonella choleraesuis and salmonella typhisuis serovars]. 19957757368
pulsed-field gel electrophoretic identification of salmonella enterica serovar typhimurium live vaccine strain zoosaloral h.salmonella enterica subsp. enterica serovar typhimurium (salm. typhimurium) live vaccine strain zoosaloral h was characterized by pulsed-field gel electrophoresis (pfge). each of the two suitable restriction enzymes, xbai and spei, produced a unique restriction fragment pattern for this live vaccine strain which was not shared by field isolates of the same serovar. the characteristic fragment pattern proved to be stable during a 22 month observation period and was also not altered after animal p ...19947765707
the sixth and seventh cholera pandemics are due to independent clones separately derived from environmental, nontoxigenic, non-o1 vibrio cholerae.the dna sequences of the asd genes from 45 isolates of vibrio cholerae (19 clinical o1 isolates, 2 environmental nontoxigenic o1 isolates, and 24 isolates with different non-o1 antigens) were determined. no differences were found within either sixth- or seventh-pandemic isolates; however, variation was found between the two forms and among the non-o1 isolates. o139 isolates had sequences identical to those of seventh-pandemic isolates. phylogenetic trees with vibrio mimicus as the outgroup sugge ...19957768818
immersion disinfection of irreversible hydrocolloid impressions. part 1: microbiology.this study evaluated the effectiveness of four disinfectants for irreversible hydrocolloid impressions. impressions were made of a sterile metal model of the maxillary arch that had been contaminated with one of the following bacteria: staphylococcus aureus, salmonella choleraesuis, pseudomonas aeruginosa, mycobacterium bovis, or bacillus subtilis. the impressions were cultured before and after immersion in one of the following disinfectants: lodofive, omc ii, 0.525% sodium hypochlorite, or alci ...19947802909
molecular evolution in the gnd locus of salmonella enterica.the gnd gene, the structural gene for 6-phosphogluconate dehydrogenase, was sequenced and analyzed in 34 isolates from different serovars of the seven subspecies of salmonella enterica to provide comparative information on the evolution in this gene, which has been studied extensively in escherichia coli. the gene tree obtained by the neighbor-joining method in general gave separate branches for each subspecies, with the few exceptions readily explained by recombination. there is evidence of rec ...19947815922
evidence for effect of random genetic drift on g+c content after lateral transfer of fucose pathway genes to escherichia coli k-12.the cps cluster of escherichia coli k-12 comprises genes involved in synthesis of capsular polysaccharide colanic acid. part of the e. coli k-12 cps region has been cloned and sequenced and compared to its salmonella enterica lt2 counterpart. the cps genes from the two organisms are homologous; in the case of the lt2 genes, with g+c content of 0.61 and codons characteristic of high g+c species, it seems clear that they have been acquired relatively recently by lateral transfer from a high g+c sp ...19947815923
development of a conjoint phage typing & biotyping schema for salmonella enterica serovar senftenberg (s. senftenberg) & the correlation of biotypes with phage types.a total of 287 strains of s. senftenberg received from various parts of india during 1969 to 1992 were phage typed using six lysogenic phages. the typability was 90.3 per cent and 14 different phage types could be defined excluding a small group of untypable strains. a biotyping scheme was developed utilising six characters and 13 biotypes could be defined. stern's glycerol medium proved to be the best discriminatory medium. diversity indeces of phage typing and biotyping schemes were 0.868 and ...19947829162
[tetracycline-resistant plasmid of salmonella enteritidis isolated in kumamoto city].we isolated salmonella choleraesuis subsp. choleraesuis serovar. enteritidis (s. enteritidis) from a mass of cases which broke out in september, 1991 in kumamoto city. the isolates were shown to hold plasmids on which a tetracycline (tc)-resistant gene was located. the plasmid, about 9 kb in size, was capable of expressing the gene in escherichia coli, unstable in s. enteritidis and escherichia coli. thus in order to investigate the creeping prevalence of the tc-resistant plasmid in kumamoto cit ...19947829903
[presentation of salmonellosis in the tandil district, argentina].from january 1990 to july 1992 a prospective study on the salmonellosis form of presentation, reservoirs and transmission in tandil was carried out. forty strains of salmonella enterica were isolated with the following distribution: 28 humans (17 children and 11 adults), 6 in foods (1 in raw milk, 3 in well water, 1 in home made mayonnaise and 1 in chicken salad) and 6 in animals (1 in cow, 2 in chicken and 2 in calves). salmonella enteritidis was the most frequent isolate. disease was evident o ...19947838978
the distribution of serotype-specific plasmids among different subgroups of strains of salmonella enterica serotype enteritidis: characterization of molecular variants by restriction enzyme fragmentation patterns.four hundred and thirty-four isolates of salmonella enterica serotype enteritidis were studied. they were grouped into five subsets defined by either the collection criteria or the parameter which formed the basis for subsequent analysis. seventy-seven per cent harboured the serotype-specific plasmid (ssp). in 55% of the isolates this was the sole plasmid. molecular variation in the ssp was detected in 17 (5%) of the isolates on the basis of restriction enzyme fragmentation pattern (refp) analys ...19957867741
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